Kenn Gerdes

Summary

Affiliation: University of Southern Denmark
Country: Denmark

Publications

  1. ncbi request reprint Prokaryotic toxin-antitoxin stress response loci
    Kenn Gerdes
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    Nat Rev Microbiol 3:371-82. 2005
  2. ncbi request reprint Bacterial mitotic machineries
    Kenn Gerdes
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, DK 5230 Odense M, Denmark
    Cell 116:359-66. 2004
  3. ncbi request reprint Overproduction of the Lon protease triggers inhibition of translation in Escherichia coli: involvement of the yefM-yoeB toxin-antitoxin system
    Susanne K Christensen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    Mol Microbiol 51:1705-17. 2004
  4. ncbi request reprint Toxin-antitoxin loci as stress-response-elements: ChpAK/MazF and ChpBK cleave translated RNAs and are counteracted by tmRNA
    Susanne K Christensen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    J Mol Biol 332:809-19. 2003
  5. doi request reprint Novel coiled-coil cell division factor ZapB stimulates Z ring assembly and cell division
    Gitte Ebersbach
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    Mol Microbiol 68:720-35. 2008
  6. ncbi request reprint Delayed-relaxed response explained by hyperactivation of RelE
    Susanne K Christensen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    Mol Microbiol 53:587-97. 2004
  7. pmc RelB and RelE of Escherichia coli form a tight complex that represses transcription via the ribbon-helix-helix motif in RelB
    Martin Overgaard
    Department of Biochemistry and Molecular Biology, University of Southern Denmark Odense, Campusvej 55, 5230 Odense M, Denmark
    J Mol Biol 394:183-96. 2009
  8. ncbi request reprint Bacterial DNA segregation by the actin-like MreB protein
    Thomas Kruse
    Department of Biochemistry and Molecular Biology, Campusvej 55, University of Southern Denmark, DK 5230 Odense M, Denmark
    Trends Cell Biol 15:343-5. 2005
  9. doi request reprint Messenger RNA interferase RelE controls relBE transcription by conditional cooperativity
    Martin Overgaard
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    Mol Microbiol 69:841-57. 2008
  10. ncbi request reprint The morphogenetic MreBCD proteins of Escherichia coli form an essential membrane-bound complex
    Thomas Kruse
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    Mol Microbiol 55:78-89. 2005

Collaborators

Detail Information

Publications37

  1. ncbi request reprint Prokaryotic toxin-antitoxin stress response loci
    Kenn Gerdes
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    Nat Rev Microbiol 3:371-82. 2005
    ..It has been proposed that toxin-antitoxin loci function in bacterial programmed cell death, but evidence now indicates that these loci provide a control mechanism that helps free-living prokaryotes cope with nutritional stress...
  2. ncbi request reprint Bacterial mitotic machineries
    Kenn Gerdes
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, DK 5230 Odense M, Denmark
    Cell 116:359-66. 2004
    ..Evidence from three different laboratories indicate that the morphogenetic MreB protein may be involved in segregation of the bacterial chromosome...
  3. ncbi request reprint Overproduction of the Lon protease triggers inhibition of translation in Escherichia coli: involvement of the yefM-yoeB toxin-antitoxin system
    Susanne K Christensen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    Mol Microbiol 51:1705-17. 2004
    ....
  4. ncbi request reprint Toxin-antitoxin loci as stress-response-elements: ChpAK/MazF and ChpBK cleave translated RNAs and are counteracted by tmRNA
    Susanne K Christensen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    J Mol Biol 332:809-19. 2003
    ..Thus, ChpAK and ChpBK inhibit translation by a mechanism very similar to that of E.coli RelE. On the basis of these results, we propose a model that integrates TA loci into general prokaryotic stress physiology...
  5. doi request reprint Novel coiled-coil cell division factor ZapB stimulates Z ring assembly and cell division
    Gitte Ebersbach
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    Mol Microbiol 68:720-35. 2008
    ..In vitro, ZapB self-assembled into long filaments and bundles. These results raise the possibility that ZapB stimulates Z ring formation directly via its capacity to self-assemble into larger structures...
  6. ncbi request reprint Delayed-relaxed response explained by hyperactivation of RelE
    Susanne K Christensen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    Mol Microbiol 53:587-97. 2004
    ..Restoration of the prestarvation level of ppGpp, in turn, allows for the resumption of stable RNA synthesis seen during the delayed-relaxed response...
  7. pmc RelB and RelE of Escherichia coli form a tight complex that represses transcription via the ribbon-helix-helix motif in RelB
    Martin Overgaard
    Department of Biochemistry and Molecular Biology, University of Southern Denmark Odense, Campusvej 55, 5230 Odense M, Denmark
    J Mol Biol 394:183-96. 2009
    ..Lon degraded RelB in vitro and degradation was inhibited by RelE, consistent with the proposal that RelE protects RelB from proteolysis by Lon in vivo...
  8. ncbi request reprint Bacterial DNA segregation by the actin-like MreB protein
    Thomas Kruse
    Department of Biochemistry and Molecular Biology, Campusvej 55, University of Southern Denmark, DK 5230 Odense M, Denmark
    Trends Cell Biol 15:343-5. 2005
    ..In particular, a recent study by Gitai et al. demonstrates that MreB specifically binds to and segregates the replication origin of the bacterial chromosome...
  9. doi request reprint Messenger RNA interferase RelE controls relBE transcription by conditional cooperativity
    Martin Overgaard
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    Mol Microbiol 69:841-57. 2008
    ..A mutational analysis of the operator sites showed that RelE in excess counteracted cooperative binding of the RelB(2)*RelE complexes to the operator sites. Thus, RelE controls relBE transcription by conditional cooperativity...
  10. ncbi request reprint The morphogenetic MreBCD proteins of Escherichia coli form an essential membrane-bound complex
    Thomas Kruse
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    Mol Microbiol 55:78-89. 2005
    ..Based on these and other observations we propose a model in which the membrane-associated MreBCD complex directs longitudinal cell wall synthesis in a process essential to maintain cell morphology...
  11. pmc Actin homolog MreB and RNA polymerase interact and are both required for chromosome segregation in Escherichia coli
    Thomas Kruse
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, DK 5230 Odense M, Denmark
    Genes Dev 20:113-24. 2006
    ..Thus, our results raise the possibility that the MreB-RNAP interaction is functionally important for chromosome segregation...
  12. ncbi request reprint Bacterial mitosis: ParM of plasmid R1 moves plasmid DNA by an actin-like insertional polymerization mechanism
    Jakob Møller-Jensen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    Mol Cell 12:1477-87. 2003
    ..coli appears to be mechanistically analogous to the actin-based motility operating in eukaryotic cells. In addition, we find evidence suggesting that plasmid pairing is required for ParM polymerization...
  13. ncbi request reprint Centromere pairing by a plasmid-encoded type I ParB protein
    Simon Ringgaard
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark
    J Biol Chem 282:28216-25. 2007
    ..These observations suggest that centromere pairing is an important intermediate step in plasmid partitioning mediated by the common type I loci...
  14. ncbi request reprint Regulatory cross-talk in the double par locus of plasmid pB171
    Simon Ringgaard
    Department of Biochemistry and Molecular Biology, Campusvej 55, University of Southern Denmark, DK 5230 Odense M, Denmark
    J Biol Chem 282:3134-45. 2007
    ..Integration host factor (IHF) was identified as a novel factor involved in par2-mediated plasmid partitioning...
  15. ncbi request reprint RelE toxins from bacteria and Archaea cleave mRNAs on translating ribosomes, which are rescued by tmRNA
    Susanne K Christensen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    Mol Microbiol 48:1389-400. 2003
    ..RelE proteins from Gram-positive Bacteria and Archaea cleaved tmRNA with a pattern similar to that of E. coli RelE, suggesting that the function and target of RelE may be conserved across the prokaryotic domains...
  16. ncbi request reprint The bacterial toxin RelE displays codon-specific cleavage of mRNAs in the ribosomal A site
    Kim Pedersen
    Department of Biochemistry and Molecular Biology, OU, University of Southern Denmark, Campusvej 55, DK 5230 Odense, Denmark
    Cell 112:131-40. 2003
    ..We suggest that inhibition of protein synthesis by RelE is reversed with the help of tmRNA, and that RelE plays a regulatory role in bacteria during adaptation to poor growth conditions...
  17. pmc Plasmid segregation: spatial awareness at the molecular level
    Jakob Møller-Jensen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    J Cell Biol 179:813-5. 2007
    ..Despite its relative simplicity, the plasmid partition spindle shares characteristics with the mitotic machinery of eukaryotic cells...
  18. ncbi request reprint Bacterial mitosis: partitioning protein ParA oscillates in spiral-shaped structures and positions plasmids at mid-cell
    Gitte Ebersbach
    Department of Biochemistry and Molecular Biology, Campusvej 55, DK 5230 Odense M, University of Southern Denmark, Denmark
    Mol Microbiol 52:385-98. 2004
    ..Therefore, our results suggest that ParA spirals position plasmids at the middle of the bacterial nucleoid and subsequently separate them into daughter cells...
  19. ncbi request reprint Regular cellular distribution of plasmids by oscillating and filament-forming ParA ATPase of plasmid pB171
    Gitte Ebersbach
    Department of Biochemistry and Molecular Biology, Campusvej 55, DK 5230 Odense M, University of Southern Denmark, Denmark
    Mol Microbiol 61:1428-42. 2006
    ..The model functions without the involvement of partition-specific host cell receptors and is thus consistent with the striking observation that partition loci can function in heterologous host organisms...
  20. ncbi request reprint Partition-associated incompatibility caused by random assortment of pure plasmid clusters
    Gitte Ebersbach
    Department of Biochemistry and Molecular Biology, Campusvej 55, DK 5230 Odense M, University of Southern Denmark, Denmark
    Mol Microbiol 56:1430-40. 2005
    ..The strength of the incompatibility was correlated with the capability of the plasmids to compete for the mid-cell position...
  21. pmc Dysfunctional MreB inhibits chromosome segregation in Escherichia coli
    Thomas Kruse
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, DK 5230 Odense M, Denmark
    EMBO J 22:5283-92. 2003
    ..The chromosomal terminus regions appeared cohered in both MreB-depleted cells and in cells overexpressing mutant forms of MreB. Our observations indicate that MreB filaments participate in directional chromosome movement and segregation...
  22. ncbi request reprint Two higBA loci in the Vibrio cholerae superintegron encode mRNA cleaving enzymes and can stabilize plasmids
    Mikkel Christensen-Dalsgaard
    Department of Biochemistry and Molecular Biology, Campusvej 55, DK 5230 Odense M, University of Southern Denmark, Denmark
    Mol Microbiol 62:397-411. 2006
    ..coli, raising the possibility that the loci contribute to maintain genetic stability of the V. cholerae superintegron. Based on these results we discuss the possible biological functions of the TA loci of V. cholerae...
  23. ncbi request reprint Plasmid segregation mechanisms
    Gitte Ebersbach
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    Annu Rev Genet 39:453-79. 2005
    ..The model is consistent with the observed movement and localization patterns of plasmid foci and does not require the involvement of plasmid-specific host-encoded factors...
  24. pmc Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes
    Deo Prakash Pandey
    Department of Biochemistry and Molecular Biology, University of Southern Denmark DK 5230 Odense M, Denmark
    Nucleic Acids Res 33:966-76. 2005
    ....
  25. ncbi request reprint Rapid induction and reversal of a bacteriostatic condition by controlled expression of toxins and antitoxins
    Kim Pedersen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    Mol Microbiol 45:501-10. 2002
    ..These results preclude that RelE and ChpAK mediate cell killing during the conditions used here. In vivo and in vitro analyses of a mutant RelE protein supported that inhibition of colony formation was due to inhibition of translation...
  26. pmc Structural analysis of the ParR/parC plasmid partition complex
    Jakob Møller-Jensen
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO J 26:4413-22. 2007
    ..This structure holds implications for how ParM polymerization drives active DNA transport during plasmid partition...
  27. pmc Spot 42 RNA mediates discoordinate expression of the E. coli galactose operon
    Thorleif Møller
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    Genes Dev 16:1696-706. 2002
    ..Our results reveal an interesting mechanism by which the expression of a promoter distal gene in an operon can be modulated and underline the importance of antisense control in bacterial gene regulation...
  28. pmc Prokaryotic DNA segregation by an actin-like filament
    Jakob Møller-Jensen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    EMBO J 21:3119-27. 2002
    ..Hence, we provide evidence for a simple prokaryotic analogue of the eukaryotic mitotic spindle apparatus...
  29. ncbi request reprint Clustering versus random segregation of plasmids lacking a partitioning function: a plasmid paradox?
    Kurt Nordström
    Department of Cell and Molecular Biology, Biomedical Center, Uppsala University, P O Box 596, Uppsala S 751 24, Sweden
    Plasmid 50:95-101. 2003
    ..In contrast, the two daughters of a Par+ plasmid are directed in opposite directions by the plasmid-encoded partition system, thereby assuring that each daughter cell receives the plasmid...
  30. ncbi request reprint RNA antitoxins
    Kenn Gerdes
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle NE2 4HH, UK
    Curr Opin Microbiol 10:117-24. 2007
    ..Thus, it is possible that an ancestral antitoxin gene evolved into the present toxin gene (symE) whose translation is repressed by an RNA antitoxin (SymR)...
  31. pmc Competitive inhibition of natural antisense Sok-RNA interactions activates Hok-mediated cell killing in Escherichia coli
    Omid R Faridani
    Department of Cell and Molecular Biology, Karolinska Institutet, Berzelius vag 35, 171 77, Stockholm, Sweden
    Nucleic Acids Res 34:5915-22. 2006
    ..Therefore, it is possible to activate suicide in bacteria by targeting antitoxins...
  32. ncbi request reprint Microbiology. Dynamic instability of a bacterial engine
    Jakob Møller-Jensen
    MRC Laboratory of Molecular Biology, Cambridge CB2 2QH, UK
    Science 306:987-9. 2004
  33. ncbi request reprint Structural and thermodynamic characterization of the Escherichia coli RelBE toxin-antitoxin system: indication for a functional role of differential stability
    Izhack Cherny
    Department of Molecular Microbiology and Biotechnology, George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    Biochemistry 46:12152-63. 2007
    ..coli is similar to ParD and CcdA antitoxins in both fold and thermodynamic properties. The differential folding state of the proteins is discussed in the context of their physiological activities...
  34. pmc F-actin-like filaments formed by plasmid segregation protein ParM
    Fusinita van den Ent
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    EMBO J 21:6935-43. 2002
    ..Thus, there is now evidence for cytoskeletal structures, formed by actin-like filaments that are involved in plasmid partitioning in E.coli...
  35. pmc Centromere parC of plasmid R1 is curved
    Christian Hoischen
    Institute for Molecular Biotechnology e V, Beutenbergstr 11, D 07745 Jena, Germany
    Nucleic Acids Res 32:5907-15. 2004
    ..Since the centromere sequence of an E.coli plasmid as well as eukaryotic centromere sequences show DNA curvature, we speculate that curvature might be a general property of centromeres...
  36. ncbi request reprint The chromosomal relBE2 toxin-antitoxin locus of Streptococcus pneumoniae: characterization and use of a bioluminescence resonance energy transfer assay to detect toxin-antitoxin interaction
    Concha Nieto
    Centro de Investigaciones Biologicas, CSIC, Ramiro de Maeztu, 9, E 28040 Madrid, Spain
    Mol Microbiol 59:1280-96. 2006
    ..This technique has shown to be amenable to a high-throughput screening (HTS), opening new avenues in the search of molecules with potential antibacterial activity able to inhibit TA interactions...
  37. ncbi request reprint A mechanism for ParB-dependent waves of ParA, a protein related to DNA segregation during cell division in prokaryotes
    Axel Hunding
    Chemistry Laboratory III, Department of Chemistry C116, H C Ørsted Institute, University of Copenhagen, Universitetsparken 5, DK 2100, Copenhagen, Denmark
    J Mol Biol 329:35-43. 2003
    ..coli and to microtubule degradation in mitosis. The study points to an ancestral role for the presented pattern types in generating bipolarity in prokaryotes and eukaryotes...