M Gajhede

Summary

Affiliation: University of Copenhagen
Country: Denmark

Publications

  1. ncbi request reprint Plant peroxidases: substrate complexes with mechanistic implications
    M Gajhede
    Protein Structure Group, Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK2100, Denmark
    Biochem Soc Trans 29:91-98. 2001
  2. ncbi request reprint Crystal structure of horseradish peroxidase C at 2.15 A resolution
    M Gajhede
    Department of Chemistry, University of Copenhagen, Denmark
    Nat Struct Biol 4:1032-8. 1997
  3. ncbi request reprint Amylosucrase, a glucan-synthesizing enzyme from the alpha-amylase family
    L K Skov
    Protein Structure Group, Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark
    J Biol Chem 276:25273-8. 2001
  4. pmc Structure of soybean seed coat peroxidase: a plant peroxidase with unusual stability and haem-apoprotein interactions
    A Henriksen
    Protein Structure Group, Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK 2100, Copenhagen, Denmark
    Protein Sci 10:108-15. 2001
  5. ncbi request reprint The structures of the horseradish peroxidase C-ferulic acid complex and the ternary complex with cyanide suggest how peroxidases oxidize small phenolic substrates
    A Henriksen
    Protein Structure Group, Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK 2100 Kobenhavn O, Denmark
    J Biol Chem 274:35005-11. 1999
  6. ncbi request reprint Major venom allergen of yellow jackets, Ves v 5: structural characterization of a pathogenesis-related protein superfamily
    A Henriksen
    Protein Structure Group, Department of Chemistry, University of Copenhagen, Copenhagen, Denmark
    Proteins 45:438-48. 2001
  7. ncbi request reprint Crystal structures of amylosucrase from Neisseria polysaccharea in complex with D-glucose and the active site mutant Glu328Gln in complex with the natural substrate sucrose
    O Mirza
    Protein Structure Group, Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark
    Biochemistry 40:9032-9. 2001
  8. ncbi request reprint Structure of barley grain peroxidase refined at 1.9-A resolution. A plant peroxidase reversibly inactivated at neutral pH
    A Henriksen
    Department of Physical Chemistry, University of Copenhagen, København O, Denmark
    J Biol Chem 273:2241-8. 1998
  9. pmc Dimerization effect of sucrose octasulfate on rat FGF1
    N Kulahin
    Protein Laboratory, Department of Neuroscience and Pharmacology, University of Copenhagen, Copenhagen, Denmark
    Acta Crystallogr Sect F Struct Biol Cryst Commun 64:448-52. 2008
  10. ncbi request reprint Pancreatic spasmolytic polypeptide: first three-dimensional structure of a member of the mammalian trefoil family of peptides
    M Gajhede
    Department of Chemistry, H C Orsted Institute, University of Copenhagen, Denmark
    Structure 1:253-62. 1993

Collaborators

Detail Information

Publications14

  1. ncbi request reprint Plant peroxidases: substrate complexes with mechanistic implications
    M Gajhede
    Protein Structure Group, Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK2100, Denmark
    Biochem Soc Trans 29:91-98. 2001
    ..A general mechanism of peroxidase substrate oxidation (compound I-->compound II and compound II-->resting state) can be proposed on the basis of the complexes and a large body of biochemical evidence...
  2. ncbi request reprint Crystal structure of horseradish peroxidase C at 2.15 A resolution
    M Gajhede
    Department of Chemistry, University of Copenhagen, Denmark
    Nat Struct Biol 4:1032-8. 1997
    ..HRPC is unique in having a ring of three peripheral Phe residues, 142, 68 and 179. These guard the entrance to the exposed haem edge. We predict that this aromatic region is important for the ability of HRPC to bind aromatic substrates...
  3. ncbi request reprint Amylosucrase, a glucan-synthesizing enzyme from the alpha-amylase family
    L K Skov
    Protein Structure Group, Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark
    J Biol Chem 276:25273-8. 2001
    ....
  4. pmc Structure of soybean seed coat peroxidase: a plant peroxidase with unusual stability and haem-apoprotein interactions
    A Henriksen
    Protein Structure Group, Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK 2100, Copenhagen, Denmark
    Protein Sci 10:108-15. 2001
    ..A contact between haem C8 vinyl and the sulphur atom of Met37 is observed in the SBP structure. This interaction might affect the stability of the haem group by stabilisation/delocalisation of the porphyrin pi-cation of compound I...
  5. ncbi request reprint The structures of the horseradish peroxidase C-ferulic acid complex and the ternary complex with cyanide suggest how peroxidases oxidize small phenolic substrates
    A Henriksen
    Protein Structure Group, Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK 2100 Kobenhavn O, Denmark
    J Biol Chem 274:35005-11. 1999
    ..Based on the observed hydrogen bonding network and previous spectroscopic and kinetic work, a general mechanism of peroxidase substrate oxidation is proposed...
  6. ncbi request reprint Major venom allergen of yellow jackets, Ves v 5: structural characterization of a pathogenesis-related protein superfamily
    A Henriksen
    Protein Structure Group, Department of Chemistry, University of Copenhagen, Copenhagen, Denmark
    Proteins 45:438-48. 2001
    ..The structure of Ves v 5 allows a detailed analysis of the epitopes that may participate in antigenic cross-reactivity, findings that are useful for the development of a vaccine for treatment of insect allergy...
  7. ncbi request reprint Crystal structures of amylosucrase from Neisseria polysaccharea in complex with D-glucose and the active site mutant Glu328Gln in complex with the natural substrate sucrose
    O Mirza
    Protein Structure Group, Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark
    Biochemistry 40:9032-9. 2001
    ..Both Asp394 and Arg446 are located in an insert connecting beta-strand 7 and alpha-helix 7 that is much longer in amylosucrase compared to other enzymes from the alpha-amylase family (family 13 of the glycoside hydrolases)...
  8. ncbi request reprint Structure of barley grain peroxidase refined at 1.9-A resolution. A plant peroxidase reversibly inactivated at neutral pH
    A Henriksen
    Department of Physical Chemistry, University of Copenhagen, København O, Denmark
    J Biol Chem 273:2241-8. 1998
    ..This is the first time that structural rearrangements linked to active site chemistry have been observed by crystallography in the peroxidase domain distal to heme...
  9. pmc Dimerization effect of sucrose octasulfate on rat FGF1
    N Kulahin
    Protein Laboratory, Department of Neuroscience and Pharmacology, University of Copenhagen, Copenhagen, Denmark
    Acta Crystallogr Sect F Struct Biol Cryst Commun 64:448-52. 2008
    ..An arginine at position 116 is a consensus residue in mammalian FGF molecules; however, it is a serine in rat FGF1. This difference may be important for SOS-mediated FGF1 dimerization in rat...
  10. ncbi request reprint Pancreatic spasmolytic polypeptide: first three-dimensional structure of a member of the mammalian trefoil family of peptides
    M Gajhede
    Department of Chemistry, H C Orsted Institute, University of Copenhagen, Denmark
    Structure 1:253-62. 1993
    ..The trefoil peptides are a rapidly growing family of peptides, mainly found in the gastrointestinal tract. There is circumstantial evidence that they stabilize the mucus layer, and may affect the rate of healing of the mucosal epithelium...
  11. ncbi request reprint A flexible loop at the dimer interface is a part of the active site of the adjacent monomer of Escherichia coli orotate phosphoribosyltransferase
    A Henriksen
    Center for Crystallographic Studies, University of Copenhagen, København, Denmark
    Biochemistry 35:3803-9. 1996
    ..coli OPRTase are found in the substrate binding regions: the 5'-phosphate binding region (residues 120-131), the binding region for the orotate part of OMP (residues 25-27), and the pyrophosphate binding region (residues 71-73)...
  12. ncbi request reprint Cyclic lipoundecapeptide amphisin from Pseudomonas sp. strain DSS73
    D Sørensen
    Marine Chemistry Section, Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK 2100 Copenhagen, Denmark
    Acta Crystallogr C 57:1123-4. 2001
    ..The structure of amphisin is mainly helical (3(10)-helix), with the cyclic peptide wrapping around a hydrogen-bonded water molecule. This lipopeptide is amphiphilic and has biosurfactant and antifungal properties...
  13. pmc Role of CD3 gamma in T cell receptor assembly
    J Dietrich
    Institute of Medical Microbiology and Immunology, University of Copenhagen, Panum Institute, Denmark
    J Cell Biol 132:299-310. 1996
    ..Furthermore, the study indicated that, in contrast to CD3 gamma, glycosylation of CD3 delta is required for TCR assembly and expression...
  14. ncbi request reprint Pancreatic spasmolytic polypeptide: crystallization, circular dichroism analysis, and preliminary X-ray diffraction studies
    M Gajhede
    Department of Physical Chemistry, H C Orsted Institute, University of Copenhagen, Denmark
    Proteins 13:364-8. 1992
    ..With this limitation, the secondary structure analysis predicts 15% alpha-helix, between 10 and 20% antiparallel beta-strand, 10% parallel beta-strand, 15% turn, and 25 to 40% of other structures...