Ditlev E Brodersen

Summary

Affiliation: University of Aarhus
Country: Denmark

Publications

  1. pmc Saccharomyces cerevisiae Ngl3p is an active 3'-5' exonuclease with a specificity towards poly-A RNA reminiscent of cellular deadenylases
    Ane Feddersen
    Department of Molecular Biology and Genetics, Centre for mRNP Biogenesis and Metabolism, Aarhus University, Gustav Wieds Vej 10c and C F Møllers Allé 130, DK 8000 Aarhus C, Denmark
    Nucleic Acids Res 40:837-46. 2012
  2. pmc Crystal structure of the VapBC toxin-antitoxin complex from Shigella flexneri reveals a hetero-octameric DNA-binding assembly
    Christian Dienemann
    Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10C, DK 8000 Aarhus C, Denmark
    J Mol Biol 414:713-22. 2011
  3. pmc The crystal structure of the intact E. coli RelBE toxin-antitoxin complex provides the structural basis for conditional cooperativity
    Andreas Bøggild
    Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology and Genetics, Aarhus University, DK 8000 Aarhus C, Denmark
    Structure 20:1641-8. 2012
  4. pmc The 1.4-A crystal structure of the S. pombe Pop2p deadenylase subunit unveils the configuration of an active enzyme
    Anette Thyssen Jonstrup
    Centre for Structural Biology, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK 8000 Arhus C, Denmark
    Nucleic Acids Res 35:3153-64. 2007
  5. pmc Mimer: an automated spreadsheet-based crystallization screening system
    Ditlev Egeskov Brodersen
    Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10C, DK 8000 Aarhus C, Denmark
    Acta Crystallogr Sect F Struct Biol Cryst Commun 69:815-20. 2013
  6. pmc The structural basis for mRNA recognition and cleavage by the ribosome-dependent endonuclease RelE
    Cajetan Neubauer
    MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
    Cell 139:1084-95. 2009
  7. ncbi The social life of ribosomal proteins
    Ditlev E Brodersen
    Centre for Structural Biology, Department of Molecular Biology, University of Aarhus, Denmark
    FEBS J 272:2098-108. 2005
  8. pmc Structure of the nuclear exosome component Rrp6p reveals an interplay between the active site and the HRDC domain
    Søren F Midtgaard
    Centre for Structural Biology, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK 8000 Aarhus C, Denmark
    Proc Natl Acad Sci U S A 103:11898-903. 2006
  9. ncbi Cyanobacteria contain a structural homologue of the Hfq protein with altered RNA-binding properties
    Andreas Bøggild
    Centre for mRNP Biogenesis and Metabolism, University of Aarhus, Denmark
    FEBS J 276:3904-15. 2009
  10. pmc Exonucleolysis is required for nuclear mRNA quality control in yeast THO mutants
    Jannie Assenholt
    Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, The Faculty of Science, Aarhus University, DK 8000 Aarhus C, Denmark
    RNA 14:2305-13. 2008

Collaborators

  • Torben Heick Jensen
  • Kenn Gerdes
  • Lori A Passmore
  • Manfred Schmid
  • Poul Valentin-Hansen
  • Poul Nissen
  • VENKATRAMAN RAMAKRISHNAN
  • Andreas Bøggild
  • Kasper R Andersen
  • Emil Dedic
  • Ane Feddersen
  • Christian Dienemann
  • Jannie Assenholt
  • Anette Thyssen Jonstrup
  • Jesper Buchhave Poulsen
  • Kehan Xu
  • Kristoffer S Winther
  • Søren Lykke-Andersen
  • Cajetan Neubauer
  • Jesper S Nielsen
  • Ann C Kelley
  • Christine M Dunham
  • Lan B Van
  • Søren F Midtgaard
  • Sabine Petry
  • Thomas Boesen
  • Emil Dandanell Agerschou
  • Lee Edward Sanderson
  • Patricia Cob-Cantal
  • Nicholas Sofos
  • Lan Bich Van
  • Esben G Poulsen
  • Ashley D Easter
  • Martin Overgaard
  • Yong Gui Gao
  • Jendrik Hentschel
  • John Mouaikel
  • Domenico Libri
  • Christian B F Andersen
  • Gorm Nielsen
  • Anders Boysen
  • Frank V Murphy
  • Maria Selmer
  • Michael J Tarry

Detail Information

Publications18

  1. pmc Saccharomyces cerevisiae Ngl3p is an active 3'-5' exonuclease with a specificity towards poly-A RNA reminiscent of cellular deadenylases
    Ane Feddersen
    Department of Molecular Biology and Genetics, Centre for mRNP Biogenesis and Metabolism, Aarhus University, Gustav Wieds Vej 10c and C F Møllers Allé 130, DK 8000 Aarhus C, Denmark
    Nucleic Acids Res 40:837-46. 2012
    ..The results suggest that Ngl3p is naturally involved in processing of poly-adenylated RNA and provide insights into the mechanistic variations observed among the redundant set of EEP enzymes found in yeast and higher eukaryotes...
  2. pmc Crystal structure of the VapBC toxin-antitoxin complex from Shigella flexneri reveals a hetero-octameric DNA-binding assembly
    Christian Dienemann
    Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10C, DK 8000 Aarhus C, Denmark
    J Mol Biol 414:713-22. 2011
    ..The structure gives new insights into the inhibition of VapC toxins by VapB and provides the molecular basis for understanding transcriptional regulation through VapB dimerization...
  3. pmc The crystal structure of the intact E. coli RelBE toxin-antitoxin complex provides the structural basis for conditional cooperativity
    Andreas Bøggild
    Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology and Genetics, Aarhus University, DK 8000 Aarhus C, Denmark
    Structure 20:1641-8. 2012
    ..The results thus provide a firm basis for understanding the model of conditional cooperativity at the molecular level...
  4. pmc The 1.4-A crystal structure of the S. pombe Pop2p deadenylase subunit unveils the configuration of an active enzyme
    Anette Thyssen Jonstrup
    Centre for Structural Biology, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK 8000 Arhus C, Denmark
    Nucleic Acids Res 35:3153-64. 2007
    ....
  5. pmc Mimer: an automated spreadsheet-based crystallization screening system
    Ditlev Egeskov Brodersen
    Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10C, DK 8000 Aarhus C, Denmark
    Acta Crystallogr Sect F Struct Biol Cryst Commun 69:815-20. 2013
    ..Automated dispensing of the resulting crystallization screens is achieved using a generic and relatively inexpensive liquid handler. ..
  6. pmc The structural basis for mRNA recognition and cleavage by the ribosome-dependent endonuclease RelE
    Cajetan Neubauer
    MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
    Cell 139:1084-95. 2009
    ..These structures provide detailed insight into the translational regulation on the bacterial ribosome by mRNA cleavage...
  7. ncbi The social life of ribosomal proteins
    Ditlev E Brodersen
    Centre for Structural Biology, Department of Molecular Biology, University of Aarhus, Denmark
    FEBS J 272:2098-108. 2005
    ..In this review we highlight some of the many known and important functions of ribosomal proteins, both during translation on the ribosome and in a wider context...
  8. pmc Structure of the nuclear exosome component Rrp6p reveals an interplay between the active site and the HRDC domain
    Søren F Midtgaard
    Centre for Structural Biology, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK 8000 Aarhus C, Denmark
    Proc Natl Acad Sci U S A 103:11898-903. 2006
    ..Finally, in vivo mutational studies show the importance of the domain contacts for the processing function of Rrp6p and highlight fundamental differences between the protein and its prokaryotic RNase D counterparts...
  9. ncbi Cyanobacteria contain a structural homologue of the Hfq protein with altered RNA-binding properties
    Andreas Bøggild
    Centre for mRNP Biogenesis and Metabolism, University of Aarhus, Denmark
    FEBS J 276:3904-15. 2009
    ..The results have implications for our understanding of the evolution of the Sm fold and the Hfq proteins in the bacterial kingdom in general...
  10. pmc Exonucleolysis is required for nuclear mRNA quality control in yeast THO mutants
    Jannie Assenholt
    Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, The Faculty of Science, Aarhus University, DK 8000 Aarhus C, Denmark
    RNA 14:2305-13. 2008
    ..Our data show that exonucleolytic attack by the nuclear exosome is needed both for provoking mRNP QC and for its ensuing elimination of faulty RNA...
  11. doi Origins and activities of the eukaryotic exosome
    Søren Lykke-Andersen
    Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, C F Møllers Allé 1130, University of Aarhus, DK 8000 Aarhus C, Denmark
    J Cell Sci 122:1487-94. 2009
    ....
  12. pmc Protein expression, crystallization and preliminary X-ray crystallographic analysis of the isolated Shigella flexneri VapC toxin
    Kehan Xu
    Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10C, DK 8000 Aarhus C, Denmark
    Acta Crystallogr Sect F Struct Biol Cryst Commun 69:762-5. 2013
    ..1, c = 52.5 Å, α = β = 90, γ = 120°. The Matthews coefficient is 2.46 Å(3) Da(-1), suggesting two molecules per asymmetric unit and corresponding to a solvent content of 50.0%...
  13. ncbi Take the "A" tail--quality control of ribosomal and transfer RNA
    Kasper R Andersen
    Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK 8000 Aarhus C, Denmark
    Biochim Biophys Acta 1779:532-7. 2008
    ..Nonetheless, our knowledge about the controlled degradation of both stable and labile RNAs is now converging into a unified picture that points to the poly(A) tail as a key discriminator of RNA quality in both bacteria and eukaryotes...
  14. ncbi Structural Characterization of the Saccharomyces cerevisiae THO Complex by Small-Angle X-Ray Scattering
    Jesper Buchhave Poulsen
    Centre for mRNP Biogenesis and Metabolism, Aarhus University, Aarhus, Denmark
    PLoS ONE 9:e103470. 2014
    ..Our model reveals not only the position of each protein in the THO complex relative to each other, but also shows that the pentamer is likely somewhat larger than what was observed by electron microscopy. ..
  15. ncbi Shape can be seductive
    Ditlev E Brodersen
    Nat Struct Biol 10:78-80. 2003
  16. ncbi Crystal structure of the 30 S ribosomal subunit from Thermus thermophilus: structure of the proteins and their interactions with 16 S RNA
    Ditlev E Brodersen
    Laboratory of Molecular Biology, MRC, Hills Road, Cambridge CB2 2QH, UK
    J Mol Biol 316:725-68. 2002
    ..We attempt to correlate the structure with a large body of biochemical and genetic data on the 30 S subunit...
  17. pmc An Hfq-like protein in archaea: crystal structure and functional characterization of the Sm protein from Methanococcus jannaschii
    Jesper S Nielsen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    RNA 13:2213-23. 2007
    ..Functional analysis reveals that Escherichia coli and M. jannaschii Hfqs display very similar biochemical and biological properties. It thus appears that the archaeal and bacterial Hfq proteins are largely functionally interchangeable...
  18. ncbi Crystal structures of the ribosome in complex with release factors RF1 and RF2 bound to a cognate stop codon
    Sabine Petry
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Cell 123:1255-66. 2005
    ..Finally, this work demonstrates the feasibility of crystallizing ribosomes with bound factors at a defined state along the translational pathway...