- Validation of suitable house keeping genes for hypoxia-cultured human chondrocytesCasper Bindzus Foldager
Orthopaedic Research Laboratory, Aarhus University Hospital, Aarhus, Denmark
BMC Mol Biol 10:94. 2009..The objective of this study was to determine hypoxia-stable housekeeping genes (HKG) for quantitative real-time PCR (qRT-PCR) in human chondrocytes cultured in 21%, 5% and 1% oxygen by geNorm and NormFinder analyses...
- Prospective 1-year follow-up study comparing joint prosthesis with tendon interposition arthroplasty in treatment of trapeziometacarpal osteoarthritisM Ulrich-Vinther
Division of Hand Surgery, Department of Orthopaedics, Aalborg Hospital, Aarhus University Hospital, Aalborg, Denmark
J Hand Surg Am 33:1369-77. 2008..Osteoarthritis of the thumb basal joint is a common and disabling condition. This clinical follow-up study compares the efficacy of total basal joint replacement surgery with that of tendon interposition arthroplasty...
- Gene therapy methods in bone and joint disorders. Evaluation of the adeno-associated virus vector in experimental models of articular cartilage disorders, periprosthetic osteolysis and bone healingMichael Ulrich-Vinther
Orthopaedic Research Group, Aarhus University Hospital University of Aarhus, Denmark
Acta Orthop Suppl 78:1-64. 2007..In principle, these problems can be overcome by delivering the relevant genes, as the therapeutic substances thereby can be persistently produced directly by local cells at the site of diseases...
- Tobacco extract but not nicotine impairs the mechanical strength of fracture healing in ratsMartin Skott
Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, Aarhus University Hospital, Norrebrogade 44, Building 1A, DK 8000 Aarhus C, Denmark
J Orthop Res 24:1472-9. 2006..Serum levels of nicotine were between 40-50 ng/mL in the group given nicotine alone and the group given tobacco extract plus nicotine (equivalent to serum levels observed in persons smoking one to two packs of cigarettes per day)...
- Gene therapy with human osteoprotegerin decreases callus remodeling with limited effects on biomechanical propertiesMichael Ulrich-Vinther
Department of Orthopaedics, Aarhus University Hospital, Noerrebrogade, DK 8000 Aarhus C, Denmark
Bone 37:751-8. 2005..039). The experiment demonstrates that AAV-OPG gene therapy decreases the fracture remodeling, but this does not influence the structural strength of healing fractures...
- Freeze-dried tendon allografts as tissue-engineering scaffolds for Gdf5 gene deliveryPatrick Basile
Center for Musculoskeletal Research, Department of Orthopaedics, University of Rochester, Rochester, New York 14642, USA
Mol Ther 16:466-73. 2008..Collectively, our data demonstrate the feasibility and efficacy of therapeutic tendon allograft processing as a novel paradigm in tissue engineering in order to address difficult clinical problems such as tendon adhesions...
- Recombinant adeno-associated virus-mediated osteoprotegerin gene therapy inhibits wear debris-induced osteolysisMichael Ulrich-Vinther
Department of Orthopedics, Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642, USA
J Bone Joint Surg Am 84:1405-12. 2002..This study investigated whether gene therapy using a recombinant adeno-associated viral vector that expresses OPG can inhibit wear debris-induced osteolysis...
- Biological effects of rAAV-caAlk2 coating on structural allograft healingMette Koefoed
The Center for Musculoskeletal Research, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA
Mol Ther 12:212-8. 2005..These results indicate that cell-free, rAAV-coated allografts have the potential to revitalize in vivo following transplantation...
- Articular cartilage biologyMichael Ulrich-Vinther
Department of Orthopedics, Arhus University Hospital, Arhus, Denmark
J Am Acad Orthop Surg 11:421-30. 2003..Tissue engineering, the combining of artificial matrices with cells and growth factors or genes, offers great potential for improving patient care...
- Effects of receptor activator of NFkappaB (RANK) signaling blockade on fracture healingLisa M Flick
Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY 14642, USA
J Orthop Res 21:676-84. 2003..Together, these data imply that osteoclast depletion via inhibition of RANK signaling is a viable option for the treatment of pathological bone loss since no adverse effects on fracture healing are observed when therapy is discontinued...
- Adeno-associated viral vector transduction of human mesenchymal stem cellsStefan Stender
Regenerative Medicine Institute, National University of Ireland, Galway, Ireland
Eur Cell Mater 13:93-9; discussion 99. 2007..Importantly, transduced MSCs retained multipotential activity comparable to untransduced controls...
- Light-activated gene transduction enhances adeno-associated virus vector-mediated gene expression in human articular chondrocytesMichael Ulrich-Vinther
The Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York 14642, USA
Arthritis Rheum 46:2095-104. 2002..To evaluate the effects of ultraviolet (UV) light as an adjuvant for recombinant adeno-associated virus (rAAV) transduction in human articular chondrocytes...