Jens Lykkesfeldt

Summary

Affiliation: The Royal Veterinary and Agricultural University
Country: Denmark

Publications

  1. ncbi Age-associated decline in ascorbic acid concentration, recycling, and biosynthesis in rat hepatocytes--reversal with (R)-alpha-lipoic acid supplementation
    J Lykkesfeldt
    Department of Molecular and Cell Biology, University of California at Berkeley, 94720, USA
    FASEB J 12:1183-9. 1998
  2. ncbi Determination of ascorbic acid and dehydroascorbic acid in biological samples by high-performance liquid chromatography using subtraction methods: reliable reduction with tris[2-carboxyethyl]phosphine hydrochloride
    J Lykkesfeldt
    Department of Pharmacology and Pathobiology, Royal Veterinary and Agricultural University, 9 Ridebanevej, Frederiksberg C, DK 1870, Denmark
    Anal Biochem 282:89-93. 2000
  3. ncbi Oxidants and antioxidants in disease: oxidative stress in farm animals
    Jens Lykkesfeldt
    Section of Biomedicine, Department of Veterinary Pathobiology, Royal Veterinary and Agricultural University, Copenhagen, Denmark
    Vet J 173:502-11. 2007
  4. ncbi Age-dependent change in Vitamin C status: a phenomenon of maturation rather than of ageing
    Jens Lykkesfeldt
    Section of Biomedicine, Department of Veterinary Pathobiology, Royal Veterinary and Agricultural University, 9 Ridebanevej, DK 1870 Frederiksberg C, Copenhagen, Denmark
    Mech Ageing Dev 126:892-8. 2005
  5. ncbi Increased oxidative damage in vitamin C deficiency is accompanied by induction of ascorbic acid recycling capacity in young but not mature guinea pigs
    Jens Lykkesfeldt
    Department of Pharmacology and Pathobiology, Royal Veterinary and Agricultural University, Frederiksberg, Copenhagen, Denmark
    Free Radic Res 36:567-74. 2002
  6. ncbi Ascorbic acid recycling in human erythrocytes is induced by smoking in vivo
    Jens Lykkesfeldt
    Department of Pharmacology and Pathobiology, Royal Veterinary and Agricultural University, Copenhagen, Denmark
    Free Radic Biol Med 35:1439-47. 2003
  7. ncbi Plasma malondialdehyde is induced by smoking: a study with balanced antioxidant profiles
    Jens Lykkesfeldt
    Section of Pharmacology, Department of Veterinary Pathobiology, Royal Veterinary and Agricultural University, 9 Ridebanevej, DK 1870 Frederiksberg C, Copenhagen, Denmark
    Br J Nutr 92:203-6. 2004
  8. doi Role of vitamin C and E supplementation on IL-6 in response to training
    Christina Yfanti
    The Centre of Inflammation and Metabolism, Department of Infectious Diseases, and Copenhagen Muscle Research Centre, Rigshospitalet, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark
    J Appl Physiol (1985) 112:990-1000. 2012
  9. doi Chronic vitamin C deficiency does not accelerate oxidative stress in ageing brains of guinea pigs
    Pernille Tveden-Nyborg
    Section of Biomedicine, Department of Disease Biology, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark
    Basic Clin Pharmacol Toxicol 110:524-9. 2012
  10. ncbi Vitamin C deficiency in weanling guinea pigs: differential expression of oxidative stress and DNA repair in liver and brain
    Jens Lykkesfeldt
    Section of Biomedicine, Department of Veterinary Pathobiology, Faculty of Life Sciences, University of Copenhagen, 9 Ridebanevej, DK 1870 Frederiksberg C, Denmark
    Br J Nutr 98:1116-9. 2007

Collaborators

Detail Information

Publications44

  1. ncbi Age-associated decline in ascorbic acid concentration, recycling, and biosynthesis in rat hepatocytes--reversal with (R)-alpha-lipoic acid supplementation
    J Lykkesfeldt
    Department of Molecular and Cell Biology, University of California at Berkeley, 94720, USA
    FASEB J 12:1183-9. 1998
    ..These results provide further evidence for the potential of alpha-lipoic acid in treatment of diseases related to oxidative stress. Furthermore, the study extends the value of ascorbic acid as a biomarker of oxidative stress...
  2. ncbi Determination of ascorbic acid and dehydroascorbic acid in biological samples by high-performance liquid chromatography using subtraction methods: reliable reduction with tris[2-carboxyethyl]phosphine hydrochloride
    J Lykkesfeldt
    Department of Pharmacology and Pathobiology, Royal Veterinary and Agricultural University, 9 Ridebanevej, Frederiksberg C, DK 1870, Denmark
    Anal Biochem 282:89-93. 2000
    ..The within- and between-day coefficients of variation for the complete assay are less than 1.5 and 3.5% for all analytes. As a whole, the method presented here is simpler and more reliable than existing methods...
  3. ncbi Oxidants and antioxidants in disease: oxidative stress in farm animals
    Jens Lykkesfeldt
    Section of Biomedicine, Department of Veterinary Pathobiology, Royal Veterinary and Agricultural University, Copenhagen, Denmark
    Vet J 173:502-11. 2007
    ..The present knowledge on oxidative stress in farm animals is the topic of this review...
  4. ncbi Age-dependent change in Vitamin C status: a phenomenon of maturation rather than of ageing
    Jens Lykkesfeldt
    Section of Biomedicine, Department of Veterinary Pathobiology, Royal Veterinary and Agricultural University, 9 Ridebanevej, DK 1870 Frederiksberg C, Copenhagen, Denmark
    Mech Ageing Dev 126:892-8. 2005
    ..05). The present data suggest that the decline in Vitamin C status with age occur early in life and is a phenomenon of maturation rather than of ageing. Data from other species and humans are discussed...
  5. ncbi Increased oxidative damage in vitamin C deficiency is accompanied by induction of ascorbic acid recycling capacity in young but not mature guinea pigs
    Jens Lykkesfeldt
    Department of Pharmacology and Pathobiology, Royal Veterinary and Agricultural University, Frederiksberg, Copenhagen, Denmark
    Free Radic Res 36:567-74. 2002
    ..The results suggest that young guinea pigs may have a more adaptable antioxidant defense system compared to mature animals while also being more susceptible to oxidative stress...
  6. ncbi Ascorbic acid recycling in human erythrocytes is induced by smoking in vivo
    Jens Lykkesfeldt
    Department of Pharmacology and Pathobiology, Royal Veterinary and Agricultural University, Copenhagen, Denmark
    Free Radic Biol Med 35:1439-47. 2003
    ..The induction of ascorbic acid recycling as a potential adaptation mechanism of the antioxidant defense to oxidative insults is discussed...
  7. ncbi Plasma malondialdehyde is induced by smoking: a study with balanced antioxidant profiles
    Jens Lykkesfeldt
    Section of Pharmacology, Department of Veterinary Pathobiology, Royal Veterinary and Agricultural University, 9 Ridebanevej, DK 1870 Frederiksberg C, Copenhagen, Denmark
    Br J Nutr 92:203-6. 2004
    ..While poor antioxidant status presumably also affects lipid peroxidation, it is only partly responsible for the increased level found in smokers in general...
  8. doi Role of vitamin C and E supplementation on IL-6 in response to training
    Christina Yfanti
    The Centre of Inflammation and Metabolism, Department of Infectious Diseases, and Copenhagen Muscle Research Centre, Rigshospitalet, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark
    J Appl Physiol (1985) 112:990-1000. 2012
    ..In conclusion, our results indicate that, although vitamin C and E supplementation may attenuate exercise-induced increases in plasma IL-6 there is no clear additive effect when combined with endurance training...
  9. doi Chronic vitamin C deficiency does not accelerate oxidative stress in ageing brains of guinea pigs
    Pernille Tveden-Nyborg
    Section of Biomedicine, Department of Disease Biology, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark
    Basic Clin Pharmacol Toxicol 110:524-9. 2012
    ..Consistent with previous findings, we show that ageing per se constitutes a considerable oxidative insult in the brain. However, our data also suggest that a long-term poor vitamin C status does not accelerate this process...
  10. ncbi Vitamin C deficiency in weanling guinea pigs: differential expression of oxidative stress and DNA repair in liver and brain
    Jens Lykkesfeldt
    Section of Biomedicine, Department of Veterinary Pathobiology, Faculty of Life Sciences, University of Copenhagen, 9 Ridebanevej, DK 1870 Frederiksberg C, Denmark
    Br J Nutr 98:1116-9. 2007
    ..Vitamin C deficiency may therefore be particularly adverse during the neonatal period...
  11. ncbi Pharmacokinetics of amoxicillin administered in drinking water to recently weaned 3- to 4-week-old pigs with diarrhea experimentally induced by Escherichia coli O149:F4
    Gerda M Jensen
    Department of Veterinary Pathobiology, The Royal Veterinary and Agricultural University, Ridebanevej 9, DK 1870 Frederiksberg C, Denmark
    Am J Vet Res 67:648-53. 2006
    ..To measure effects of Escherichia coli O149:F4-induced diarrhea on water consumption and pharmacokinetics of amoxicillin after administration in drinking water...
  12. doi High dietary fat and cholesterol exacerbates chronic vitamin C deficiency in guinea pigs
    Henriette Frikke-Schmidt
    Section of Biomedicine, Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Ridebanevej 9, DK 1870 Frederiksberg C, Denmark
    Br J Nutr 105:54-61. 2011
    ..The present findings suggest that this type of diet increases the turnover of Asc; hence, individuals consuming high-lipid diets may be at increased risk of vitamin C deficiency...
  13. doi Poor antioxidant status exacerbates oxidative stress and inflammatory response to Pseudomonas aeruginosa lung infection in guinea pigs
    Peter Ø Jensen
    Department of Clinical Microbiology, University Hospital, Copenhagen, Denmark
    Basic Clin Pharmacol Toxicol 110:353-8. 2012
    ..Our data show that poor antioxidant status exacerbates the outcome of biofilm-related infections...
  14. ncbi Gamma-tocopherol supplementation inhibits protein nitration and ascorbate oxidation in rats with inflammation
    Qing Jiang
    Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA
    Free Radic Biol Med 33:1534-42. 2002
    ....
  15. ncbi Pharmacokinetics of amoxicillin after oral administration in recently weaned piglets with experimentally induced Escherichia coli subtype O149:F4 diarrhea
    Gerda M Jensen
    Department of Pharmacology and Pathobiology, Royal Veterinary and Agricultural University, Bulowsvej 13, DK 1870 Frederiksberg C, Denmark
    Am J Vet Res 65:992-5. 2004
    ..To measure the effect of Escherichia coli subtype O149:F4-induced diarrhea on the pharmacokinetics of orally administered amoxicillin in affected piglets relative to that of uninfected piglets...
  16. ncbi Effect of smoking on erythorbic acid pharmacokinetics
    Jens Lykkesfeldt
    Department of Pharmacology and Pathobiology, Royal Veterinary and Agricultural University, Copenhagen, Denmark
    Br J Nutr 89:667-71. 2003
    ....
  17. doi Effect of vitamin C and iron chelation on diesel exhaust particle and carbon black induced oxidative damage and cell adhesion molecule expression in human endothelial cells
    Henriette Frikke-Schmidt
    Section of Biomedicine, Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Denmark
    Toxicol Lett 203:181-9. 2011
    ..The study shows that exposure to carbon black and DEP in HUVECs can generate both oxidative stress and expression of cell surface adhesion molecules and that these effects can in part be attenuated by vitamin C and desferrioxamine...
  18. ncbi Antioxidant supplementation does not alter endurance training adaptation
    Christina Yfanti
    Center of Inflammation and Metabolism at Department of Infectious Diseases, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    Med Sci Sports Exerc 42:1388-95. 2010
    ..However, RONS production may also activate redox-sensitive signaling pathways and transcription factors, which subsequently, may promote training adaptation...
  19. ncbi Effects of heparin-binding protein (CAP37/azurocidin) in a porcine model of Actinobacillus pleuropneumoniae-induced pneumonia
    Brian Lauritzen
    Department of Pharmacology and Pathobiology, The Royal Veterinary and Agricultural University, Copenhagen, Denmark
    Pharmacol Res 51:509-14. 2005
    ..No signs of adverse effects of the HBP-infusion were observed. More studies are needed to elucidate of the effects of HBP further and to optimise the dosage regimen for further improvement the efficacy and safety...
  20. doi Role of marginal vitamin C deficiency in atherogenesis: in vivo models and clinical studies
    Henriette Frikke-Schmidt
    Section of Biomedicine, Department of Disease Biology, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Denmark
    Basic Clin Pharmacol Toxicol 104:419-33. 2009
    ..In this perspective, the present review examines the evidence for a specific link between vitamin C deficiency and increased risk of atherosclerosis as well as the possible mechanisms by which vitamin C may exert its protective function...
  21. ncbi Ascorbate and dehydroascorbic acid as reliable biomarkers of oxidative stress: analytical reproducibility and long-term stability of plasma samples subjected to acidic deproteinization
    Jens Lykkesfeldt
    Section of Biomedicine, Department of Veterinary Pathobiology, Faculty of Life Sciences, University of Copenhagen, 9 Ridebanevej DK 1870, Frederiksberg, Copenhagen, Denmark
    Cancer Epidemiol Biomarkers Prev 16:2513-6. 2007
    ..In conclusion, ascorbate and dehydroascorbic acid could be adequately preserved in plasma stored at -80 degrees C following acidic deproteinization with meta-phosphoric acid containing 2 mmol/L of EDTA...
  22. ncbi Malondialdehyde as biomarker of oxidative damage to lipids caused by smoking
    Jens Lykkesfeldt
    Section of Biomedicine, Department of Veterinary Pathobiology, Faculty of Life Sciences, University of Copenhagen, 9 Ridebanevej, DK 1870 Frederiksberg C, Denmark
    Clin Chim Acta 380:50-8. 2007
    ....
  23. ncbi Oxidative stress and damage in liver, but not in brain, of Fischer 344 rats subjected to dietary iron supplementation with lipid-soluble [(3,5,5-trimethylhexanoyl)ferrocene]
    Jens Lykkesfeldt
    Section of Biomedicine, Department of Veterinary Pathobiology, University of Copenhagen, Denmark
    J Biochem Mol Toxicol 21:145-55. 2007
    ....
  24. doi Prolonged maternal vitamin C deficiency overrides preferential fetal ascorbate transport but does not influence perinatal survival in guinea pigs
    Janne G Schjoldager
    Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 9 Ridebanevej, DK 1870 Frederiksberg C, Denmark
    Br J Nutr 110:1573-9. 2013
    ..This contradicts the notion that a fetus is protected from vitC deficiency by the placental Na-dependent vitC co-transporter, SVCT2, thus fetal development may be susceptible to the negative effects of maternal vitC deficiency...
  25. doi Ascorbate and dehydroascorbic acid as biomarkers of oxidative stress: validity of clinical data depends on vacutainer system used
    Jens Lykkesfeldt
    Section of Biomedicine, Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, DK 1870 Frederiksberg C, Denmark
    Nutr Res 32:66-9. 2012
    ..In comparison, K(3)-EDTA vacutainers were superior in maintaining low ex vivo oxidation of vitamin C...
  26. doi Fluctuating hyperglycaemia increases oxidative stress response in lean rats compared to sustained hyperglycaemia despite lower glycaemic exposure
    Günaj Rakipovski
    Section of Biomedicine, Department of Veterinary Disease Biology, University of Copenhagen, Denmark
    Diab Vasc Dis Res 8:295-8. 2011
    ..To compare the effect of fluctuating glucose with sustained hyperglycaemia on systemic oxidative stress during 72 h of glucose infusion...
  27. pmc Maternal vitamin C deficiency during pregnancy persistently impairs hippocampal neurogenesis in offspring of guinea pigs
    Pernille Tveden-Nyborg
    Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
    PLoS ONE 7:e48488. 2012
    ..Our findings place attention on a yet unrecognized consequence of marginal VitC deficiency during pregnancy...
  28. doi Does vitamin C deficiency result in impaired brain development in infants?
    Pernille Tveden-Nyborg
    Section of Biomedicine, Department of Disease Biology, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark
    Redox Rep 14:2-6. 2009
    ..Recent data from our laboratory suggest that the neonatal brain is particularly susceptible to vitamin C deficiency and that this condition may adversely affect early brain development...
  29. ncbi Evaluation of a single dose versus a divided dose regimen of danofloxacin in treatment of Actinobacillus pleuropneumoniae infection in pigs
    Brian Lauritzen
    Department of Pharmacology and Pathobiology, The Royal Veterinary and Agricultural University, 9 Ridebanevej, 1870 Frederiksberg C, Copenhagen, Denmark
    Res Vet Sci 74:271-7. 2003
    ..The use of the more convenient single dose regimen was appropriate, as it was at least equivalent to the fractionated regimen...
  30. pmc Whey protein reduces early life weight gain in mice fed a high-fat diet
    Britt Tranberg
    Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C, Denmark
    PLoS ONE 8:e71439. 2013
    ..These findings could not be explained by changes in food intake or gut microbiota composition. Further studies are needed to clarify the mechanisms behind the metabolic effects of whey. ..
  31. doi Keeping the intracellular vitamin C at a physiologically relevant level in endothelial cell culture
    Henriette Frikke-Schmidt
    Section of Biomedicine, Department of Disease Biology, Faculty of Life Sciences, University of Copenhagen, Frederiksberg CDK 1870, Denmark
    Anal Biochem 397:135-7. 2010
    ..We found that a single supplement of ascorbate and 2-phosphoascorbate adequately maintains intracellular vitamin C at physiological levels for up to 72 h...
  32. doi Nephrectomized and hepatectomized animal models as tools in preclinical pharmacokinetics
    Bill Vestergaard
    Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
    Basic Clin Pharmacol Toxicol 113:75-86. 2013
    ..The present MiniReview discusses pros and cons of the available techniques associated with establishing nephrectomy and hepatectomy models. ..
  33. doi Does vitamin C deficiency increase lifestyle-associated vascular disease progression? Evidence based on experimental and clinical studies
    Pernille Tveden-Nyborg
    Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark
    Antioxid Redox Signal 19:2084-104. 2013
    ..Despite continuous advances in the prevention of cardiovascular disease (CVD), critical issues associated with an unhealthy lifestyle remain an increasing cause of morbidity and mortality in industrialized countries...
  34. doi Kinetics of acid-induced degradation of tetra- and dihydrobiopterin in relation to their relevance as biomarkers of endothelial function
    Alan Mortensen
    Faculty of Health and Medical Sciences, Department of Veterinary Disease Biology, University of Copenhagen, Copenhagen, Denmark
    Biomarkers 18:55-62. 2013
    ..Based on our results, we are able to recommend that blood samples are immediately stabilized with dithioerythriol and protein precipitation conducted using trichloroacetic acid (TCA)...
  35. pmc Feeding acetyl-L-carnitine and lipoic acid to old rats significantly improves metabolic function while decreasing oxidative stress
    Tory M Hagen
    Department of Biochemistry and Biophysics, Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA
    Proc Natl Acad Sci U S A 99:1870-5. 2002
    ..Feeding ALCAR in combination with LA increased metabolism and lowered oxidative stress more than either compound alone...
  36. ncbi Cerebral vasculature is the major target of oxidative protein alterations in bacterial meningitis
    Manuela Schaper
    Institute for Infectious Diseases, University of Berne, Switzerland
    J Neuropathol Exp Neurol 61:605-13. 2002
    ....
  37. ncbi Vitamin C supplementation decreases oxidative DNA damage in mononuclear blood cells of smokers
    Peter Møller
    Institute of Public Health c o Dept of Pharmacology, The Panum Institute, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen, Denmark
    Eur J Nutr 43:267-74. 2004
    ..Such effects have been shown in mononuclear blood cells in the first few hours after ingestion, whereas studies of longer-term effects in well-nourished humans have been mainly negative...
  38. ncbi Vitamin C pharmacokinetics of plain and slow release formulations in smokers
    Michael Viscovich
    Department of Clinical Pharmacology Q 7642, Rigshospitalet, Copenhagen University Hospital, Blegdamsvej 9, DK 2100, Denmark
    Clin Nutr 23:1043-50. 2004
    ....
  39. ncbi Clindamycin is neuroprotective in experimental Streptococcus pneumoniae meningitis compared with ceftriaxone
    Tobias Böttcher
    Department of Neurology, University of Rostock, Rostock, Germany
    J Neurochem 91:1450-60. 2004
    ..This study suggests neuroprotection to be a group effect of bactericidal protein synthesis-inhibiting antimicrobial agents compared with the standard therapy with beta-lactam antibiotics in meningitis...
  40. ncbi Oxidative stress precedes peak systemic inflammatory response in pediatric patients undergoing cardiopulmonary bypass operation
    Stephan Christen
    Institute for Infectious Diseases, University of Berne, Friedbuehlstrasse 51, CH 3010 Berne, Switzerland
    Free Radic Biol Med 38:1323-32. 2005
    ..g., off-pump surgery) or the resultant oxidative stress (e.g., antioxidant treatment) should be considered as possible strategies to lower the rate of postoperative complications in pediatric CPB...
  41. ncbi Antioxidant supplementation enhances the exercise-induced increase in mitochondrial uncoupling protein 3 and endothelial nitric oxide synthase mRNA content in human skeletal muscle
    Ylva Hellsten
    Department of Exercise and Sport Science, Copenhagen Muscle Research Center, University of Copenhagen, DK 2100 Copenhagen, Denmark
    Free Radic Biol Med 43:353-61. 2007
    ..The data show that ROS are formed within skeletal muscle during exercise and that oral intake of antioxidants can enhance the exercise-induced adaptive mRNA responses of eNOS and UCP3...
  42. ncbi Long-term high copper intake: effects on indexes of copper status, antioxidant status, and immune function in young men
    Judith R Turnlund
    US Department of Agriculture, Agricultural Research Service, Western Human Nutrition Research Center, University of California, Davis 95616, USA
    Am J Clin Nutr 79:1037-44. 2004
    ..Short-term high copper intake does not appear to affect indexes of copper status or functions related to copper status, but the effects of long-term high copper intake are unknown...
  43. ncbi Electroencephalographic changes after pediatric cardiac surgery with cardiopulmonary bypass: is slow wave activity unfavorable?
    Bernard Schmitt
    University Children s Hospital, Zurich, Switzerland
    Pediatr Res 58:771-8. 2005
    ..However, our data do not support the notion that EEG slowing is associated with adverse neurologic outcome. This is supported by the fact that EEG slowing was associated with less oxido-inflammatory stress...
  44. doi Utility of melatonin to treat surgical stress after major vascular surgery--a safety study
    Bülent Kücükakin
    Department of Surgical Gastroenterology D, University of Copenhagen, Gentofte Hospital, Hellerup, Denmark
    J Pineal Res 44:426-31. 2008
    ..Melatonin may decrease oxidative damage resulting from surgery, but randomized clinical trials are required before definitive conclusions can be drawn regarding the clinical benefit of melatonin in surgical situations...