Barbara Ann Halkier

Summary

Affiliation: The Royal Veterinary and Agricultural University
Country: Denmark

Publications

  1. pmc USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products
    Fernando Geu-Flores
    Plant Biochemistry Laboratory, Institute of Plant Biology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, Frederiksberg C, Denmark
    Nucleic Acids Res 35:e55. 2007
  2. pmc Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments
    Hussam H Nour-Eldin
    Plant Biochemistry Laboratory, Center for Molecular Plant Physiology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK 1871 Frederiksberg C, Denmark
    Nucleic Acids Res 34:e122. 2006
  3. ncbi request reprint Biology and biochemistry of glucosinolates
    Barbara Ann Halkier
    Plant Biochemistry Laboratory, Department of Plant Biology, Royal Veterinary and Agricultural University, DK 1871 Frederiksberg C, Denmark
    Annu Rev Plant Biol 57:303-33. 2006
  4. pmc Integration of biosynthesis and long-distance transport establish organ-specific glucosinolate profiles in vegetative Arabidopsis
    Tonni Grube Andersen
    DynaMo Centre of Excellence, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871 Frederiksberg C, Denmark
    Plant Cell 25:3133-45. 2013
  5. pmc Cytosolic γ-glutamyl peptidases process glutathione conjugates in the biosynthesis of glucosinolates and camalexin in Arabidopsis
    Fernando Geu-Flores
    Section for Molecular Plant Biology, Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, 1871 Frederiksberg, Denmark
    Plant Cell 23:2456-69. 2011
  6. doi request reprint Towards engineering glucosinolates into non-cruciferous plants
    Fernando Geu-Flores
    Plant Biochemistry Laboratory, VKR Research Centre Pro Active Plants, Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, 40 Thorvaldsensvej, 1871, Frederiksberg C, Denmark
    Planta 229:261-70. 2009
  7. doi request reprint Microbial production of indolylglucosinolate through engineering of a multi-gene pathway in a versatile yeast expression platform
    Michael Dalgaard Mikkelsen
    University of Copenhagen, Faculty of Life Sciences, Department of Plant Biology and Biotechnology, Molecular Plant Biology, VKR Research Centre for Pro Active Plants, 40 Thorvaldsensvej, Frederiksberg C, Copenhagen, Denmark
    Metab Eng 14:104-11. 2012
  8. pmc A complex interplay of three R2R3 MYB transcription factors determines the profile of aliphatic glucosinolates in Arabidopsis
    Ida Elken Sønderby
    Plant Biochemistry Laboratory, Villum Kann Rasmussen Research Centre for Pro Active Plants, and Plant Biochemistry Laboratory, Department of Plant Biology and Biotechnology, Faculty of Life Sciences, Copenhagen University, 1871 Frederiksberg C, Copenhagen, Denmark
    Plant Physiol 153:348-63. 2010
  9. doi request reprint NRT/PTR transporters are essential for translocation of glucosinolate defence compounds to seeds
    Hussam Hassan Nour-Eldin
    Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871Frederiksberg C, Denmark
    Nature 488:531-4. 2012
  10. doi request reprint The emerging field of transport engineering of plant specialized metabolites
    Hussam Hassan Nour-Eldin
    DynaMo Centre of Excellence, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark
    Curr Opin Biotechnol 24:263-70. 2013

Collaborators

  • Danielj Kliebenstein
  • Fernando Geu-Flores
  • Ingo Dreyer
  • Jing Li
  • Morten H H Nørholm
  • Christoph Bottcher
  • Jonathan Gershenzon
  • Rainer Hedrich
  • Michael Dalgaard Mikkelsen
  • Carl Erik Olsen
  • Hussam Hassan Nour-Eldin
  • Bjarne Gram Hansen
  • Ida Elken Sønderby
  • Tonni Grube Andersen
  • Meike Burow
  • Bent Larsen Petersen
  • Peter Naur
  • Heather C Rowe
  • Carla Ticconi
  • Adam M Wentzell
  • Hussam H Nour-Eldin
  • Günter Brader
  • Titima Tantikanjana
  • Victoria Louise Fuller
  • Uffe Hasbro Mortensen
  • Dietmar Geiger
  • Line Due Buron
  • Svend Roesen Madsen
  • Bo Salomonsen
  • Morten Egevang Jørgensen
  • Henrik Bjørn Nielsen
  • Majse Nafisi
  • Victoria L Fuller
  • Nanna Bjarnholt
  • Jacob K Jensen
  • E Tapio Palva
  • Bjarne G Hansen
  • Venkatesan Sundaresan
  • Mumtaz Hussain
  • Erik Andreasson
  • Hasse Rasmussen
  • Anders Bøgh Jensen
  • Erich Glawischnig
  • Søren Bak
  • Sixue Chen
  • Carsten Hørslev Hansen

Detail Information

Publications23

  1. pmc USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products
    Fernando Geu-Flores
    Plant Biochemistry Laboratory, Institute of Plant Biology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, Frederiksberg C, Denmark
    Nucleic Acids Res 35:e55. 2007
    ..We expect this method to replace overlapping PCR and the use of type IIS restriction enzymes in many of their applications...
  2. pmc Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments
    Hussam H Nour-Eldin
    Plant Biochemistry Laboratory, Center for Molecular Plant Physiology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK 1871 Frederiksberg C, Denmark
    Nucleic Acids Res 34:e122. 2006
    ..A comprehensive set of vectors has been constructed covering a wide range of different applications and their functionality has been confirmed...
  3. ncbi request reprint Biology and biochemistry of glucosinolates
    Barbara Ann Halkier
    Plant Biochemistry Laboratory, Department of Plant Biology, Royal Veterinary and Agricultural University, DK 1871 Frederiksberg C, Denmark
    Annu Rev Plant Biol 57:303-33. 2006
    ..This may enable us to more fully exploit the potential of these compounds in agriculture and medicine...
  4. pmc Integration of biosynthesis and long-distance transport establish organ-specific glucosinolate profiles in vegetative Arabidopsis
    Tonni Grube Andersen
    DynaMo Centre of Excellence, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871 Frederiksberg C, Denmark
    Plant Cell 25:3133-45. 2013
    ....
  5. pmc Cytosolic γ-glutamyl peptidases process glutathione conjugates in the biosynthesis of glucosinolates and camalexin in Arabidopsis
    Fernando Geu-Flores
    Section for Molecular Plant Biology, Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, 1871 Frederiksberg, Denmark
    Plant Cell 23:2456-69. 2011
    ....
  6. doi request reprint Towards engineering glucosinolates into non-cruciferous plants
    Fernando Geu-Flores
    Plant Biochemistry Laboratory, VKR Research Centre Pro Active Plants, Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, 40 Thorvaldsensvej, 1871, Frederiksberg C, Denmark
    Planta 229:261-70. 2009
    ..In summary, we transferred the last three steps of the benzylglucosinolate pathway to tobacco as a first step towards engineering glucosinolates into non-cruciferous plants...
  7. doi request reprint Microbial production of indolylglucosinolate through engineering of a multi-gene pathway in a versatile yeast expression platform
    Michael Dalgaard Mikkelsen
    University of Copenhagen, Faculty of Life Sciences, Department of Plant Biology and Biotechnology, Molecular Plant Biology, VKR Research Centre for Pro Active Plants, 40 Thorvaldsensvej, Frederiksberg C, Copenhagen, Denmark
    Metab Eng 14:104-11. 2012
    ..Production of indolylglucosinolate serves as a proof-of-concept for our expression platform, and provides a basis for large-scale microbial production of specific glucosinolates for the benefit of human health...
  8. pmc A complex interplay of three R2R3 MYB transcription factors determines the profile of aliphatic glucosinolates in Arabidopsis
    Ida Elken Sønderby
    Plant Biochemistry Laboratory, Villum Kann Rasmussen Research Centre for Pro Active Plants, and Plant Biochemistry Laboratory, Department of Plant Biology and Biotechnology, Faculty of Life Sciences, Copenhagen University, 1871 Frederiksberg C, Copenhagen, Denmark
    Plant Physiol 153:348-63. 2010
    ..Similar regulatory networks might exist in other secondary pathways...
  9. doi request reprint NRT/PTR transporters are essential for translocation of glucosinolate defence compounds to seeds
    Hussam Hassan Nour-Eldin
    Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871Frederiksberg C, Denmark
    Nature 488:531-4. 2012
    ..Identification of the glucosinolate transporters has agricultural potential as a means to control allocation of defence compounds in a tissue-specific manner...
  10. doi request reprint The emerging field of transport engineering of plant specialized metabolites
    Hussam Hassan Nour-Eldin
    DynaMo Centre of Excellence, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark
    Curr Opin Biotechnol 24:263-70. 2013
    ..As glucosinolates are specialized metabolites of the model plant Arabidopsis, glucosinolate transport processes emerge as a model system for studying transport of specialized metabolites...
  11. ncbi request reprint Production of the cancer-preventive glucoraphanin in tobacco
    Michael Dalgaard Mikkelsen
    University of Copenhagen, Faculty of Life Sciences, Department of Plant Biology, Plant Biochemistry Laboratory, VKR Research Centre for Pro Active Plants, 40 Thorvaldsensvej, DK 1871 Frederiksberg C, Copenhagen, Denmark
    Mol Plant 3:751-9. 2010
    ..Demonstrating the production of the high-value glucoraphanin in a heterologous host has great potential in the food and medicinal industry as a means to generate a stable, rich source of glucoraphanin for the benefit of human health...
  12. doi request reprint Controlled indole-3-acetaldoxime production through ethanol-induced expression of CYP79B2
    Michael Dalgaard Mikkelsen
    VKR Research Centre Pro Active Plants, Department of Plant Biology and Biotechnology, University of Copenhagen, 40 Thorvaldsensvej, 1871, Frederiksberg C, Denmark
    Planta 229:1209-17. 2009
    ..The transgenic mutant lines are thus promising tools for elucidating the interplay between primary and secondary metabolism...
  13. pmc Subclade of flavin-monooxygenases involved in aliphatic glucosinolate biosynthesis
    Jing Li
    Plant Biochemistry Laboratory, Department of Plant Biology and Villum Kann Rasmussen Research Centre for Pro Active Plants, Faculty of Life Sciences, University of Copenhagen, DK 1871 Frederiksberg C, Denmark
    Plant Physiol 148:1721-33. 2008
    ....
  14. doi request reprint Glucosinolate engineering identifies a gamma-glutamyl peptidase
    Fernando Geu-Flores
    Plant Biochemistry Laboratory, Department of Plant Biology, Faculty of Life Sciences, University of Copenhagen, Frederiksberg, Denmark
    Nat Chem Biol 5:575-7. 2009
    ....
  15. pmc A systems biology approach identifies a R2R3 MYB gene subfamily with distinct and overlapping functions in regulation of aliphatic glucosinolates
    Ida Elken Sønderby
    Plant Biochemistry Laboratory, Department of Plant Biology, Center for Molecular Plant Physiology PlaCe, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark
    PLoS ONE 2:e1322. 2007
    ..Knowledge about the genes controlling glucosinolate regulation is limited. Here, we identify three R2R3 MYB transcription factors regulating aliphatic glucosinolate biosynthesis in Arabidopsis by combining several systems biology tools...
  16. ncbi request reprint Arabidopsis mutants in the C-S lyase of glucosinolate biosynthesis establish a critical role for indole-3-acetaldoxime in auxin homeostasis
    Michael Dalgaard Mikkelsen
    Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK 1871 Frederiksberg C, Copenhagen, Denmark
    Plant J 37:770-7. 2004
    ..Our findings provide important insight to the critical role IAOx plays in auxin homeostasis as a key branching point between primary and secondary metabolism, and define a framework for further dissection of auxin biosynthesis...
  17. pmc CYP83A1 and CYP83B1, two nonredundant cytochrome P450 enzymes metabolizing oximes in the biosynthesis of glucosinolates in Arabidopsis
    Peter Naur
    Plant Biochemistry Laboratory, Department of Chemistry, Center for Molecular Plant Physiology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK 1871 Frederiksberg C, Denmark
    Plant Physiol 133:63-72. 2003
    ....
  18. pmc Metabolic engineering of valine- and isoleucine-derived glucosinolates in Arabidopsis expressing CYP79D2 from Cassava
    Michael Dalgaard Mikkelsen
    Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, DK 1871 Frederiksberg C, Copenhagen, Denmark
    Plant Physiol 131:773-9. 2003
    ....
  19. pmc Modulation of CYP79 genes and glucosinolate profiles in Arabidopsis by defense signaling pathways
    Michael Dalgaard Mikkelsen
    Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, opgang 10, St, DK 1871 Frederiksberg C, Copenhagen, Denmark
    Plant Physiol 131:298-308. 2003
    ..Thus, different defense pathways activate subsets of biosynthetic enzymes, leading to the accumulation of specific glucosinolates...
  20. ncbi request reprint Composition and content of glucosinolates in developing Arabidopsis thaliana
    Bent Larsen Petersen
    Plant Biochemistry Laboratory, Department of Plant Biology, Royal Veterinary and Agricultural University, Frederiksberg C, Copenhagen, Denmark
    Planta 214:562-71. 2002
    ..This indicates that there is a continuous turnover of glucosinolates during development and not only upon tissue disruption...
  21. pmc Linking metabolic QTLs with network and cis-eQTLs controlling biosynthetic pathways
    Adam M Wentzell
    Department of Plant Sciences, University of California Davis, Davis, California, USA
    PLoS Genet 3:1687-701. 2007
    ..This suggests that natural variation in transcripts may significantly impact phenotypic variation, but that natural variation in metabolites or their enzymatic loci can feed back to affect the transcripts...
  22. ncbi request reprint Altering glucosinolate profiles modulates disease resistance in plants
    Günter Brader
    Division of Genetics, Department of Biological and Environmental Sciences, Faculty of Biosciences, Viikki Biocenter, University of Helsinki, PO Box 56, FIN 00014 Helsinki, Finland
    Plant J 46:758-67. 2006
    ..Arabidopsis with modified GS profiles provide important tools for evaluating the biological effects of individual GSs and thereby show potential as biotechnological tools for the generation of plants with tailor-made disease resistance...
  23. pmc Functional analysis of the tandem-duplicated P450 genes SPS/BUS/CYP79F1 and CYP79F2 in glucosinolate biosynthesis and plant development by Ds transposition-generated double mutants
    Titima Tantikanjana
    Department of Plant Biology and Agronomy, University of California, Davis, California 95616, USA
    Plant Physiol 135:840-8. 2004
    ..This study also provides further demonstration of how gluconisolate biosynthesis, regarded as secondary metabolism, is intricately linked with hormone homeostatis and hence with plant growth and development...