Research Topics
| P ChristensenSummaryAffiliation: The Royal Veterinary and Agricultural University Country: Denmark Publications
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Detail Information
Publications
A flow cytometric method for rapid determination of sperm concentration and viability in mammalian and avian semenPreben Christensen
Department of Production Animals and Horses, Section for Reproduction, Royal Veterinary and Agricultural University, Frederiksberg, Denmark
J Androl 25:255-64. 2004..In addition, the FACSCount AF flow cytometer determined both sperm concentration (coefficient of variation 3.3%) and sperm viability (coefficient of variation 0.7%) with high precision...
Discrepancies in the determination of sperm concentration using Bürker-Türk, Thoma and Makler counting chambersP Christensen
Department of Large Animal Sciences, Veterinary Reproduction and Obstetrics, Royal Veterinary and Agricultural University, 1870 Frederiksberg C, Denmark
Theriogenology 63:992-1003. 2005..It is concluded that duplicate counts by at least two technicians is recommended to achieve high precision but, that particular caution should be exerted with regard to the precision and accuracy of the used counting device...
Relationship between sperm viability as determined by flow cytometry and nonreturn rate of dairy bullsPreben Christensen
Department of Large Animal Sciences, Veterinary Reproduction and Obstetrics, Royal Veterinary and Agricultural University, Dyrlaegevej 68, 1870 Frederiksberg C, Denmark
J Androl 26:98-106. 2005..Flow cytometric results for 1 extender (Biociphos Plus) indicated poorer sperm survival during postthaw incubation compared with Triladyl extender with whole and with clarified egg yolk...
Implementation of flow cytometry for quality control in four Danish bull studsP Christensen
Department of Large Animal Sciences, Veterinary Reporduction and Obstetrics, The Royal Veterinary and Agricultural University, Dyrlaegevej 68, 1870 Frederiksberg C, Denmark
Anim Reprod Sci 85:201-8. 2005..It is concluded that flow cytometric determination of sperm concentration and viability can be used to improve semen assessment by AI studs and result in a better quality control...
Quality control in boar semen production by use of the FACSCount AF systemPreben Christensen
Section for Reproduction, Department of Large Animal Sciences, Royal Veterinary and Agricultural University, Dyrlaegevej 68, 1870 Frederiksberg C, Denmark
Theriogenology 62:1218-28. 2004..It is concluded that the FACSCount AF flow cytometer is a strong tool for improvement of the quality control in artificial insemination (AI) centres...
Relationship among seminal quality measures and field fertility of young dairy bulls using low-dose inseminationsP Christensen
Copenhagen University, Faculty of Life Sciences, Department of Large Animal Sciences, Veterinary Reproduction and Obstetrics, DK 1870 Frederiksberg, Denmark
J Dairy Sci 94:1744-54. 2011....
Validation of the FACSCount AF system for determination of sperm concentration in boar semenC Hansen
Department of Clinical Studies, Section for Reproduction, Royal Veterinary and Agricultural University, Frederiksberg, Denmark
Reprod Domest Anim 37:330-4. 2002....
In vitro induction of the acrosome reaction in bull sperm and the relationship to field fertility using low-dose inseminationsA Birck
Copenhagen University, Faculty of Life Sciences, Department of Large Animal Sciences, Veterinary Reproduction and Obstetrics, Frederiksberg, Denmark
Theriogenology 73:1180-91. 2010..The current results therefore indicate that this sperm parameter is important in the field and also may play a role in the IVF laboratory...
Sperm chromatin structure integrity in liquid stored boar semen and its relationships with field fertilityG B Boe-Hansen
Section for Veterinary Reproduction and Obstetrics, Department of Large Animal Sciences, Faculty of Life Sciences, University of Copenhagen, Dyrlaegevej 68, 1870 Frederiksberg, Denmark
Theriogenology 69:728-36. 2008..In conclusion the SCSA technique appears to be able to identify individuals with lower fertility with respect to litter size, and could in the future be implemented by the pig industry after a cost-benefit analysis...
Dynamics of the induced acrosome reaction in boar sperm evaluated by flow cytometryAnders Birck
Copenhagen University, Faculty of Life Sciences, Department of Large Animal Sciences, Veterinary Reproduction and Obstetrics, Frederiksberg, Denmark
Anim Reprod Sci 115:124-36. 2009..The results lead to suggestions for an optimized AR induction protocol that takes both sperm viability and the effectiveness of AR induction into consideration...
The sperm chromatin structure assay as a diagnostic tool in the human fertility clinicGry Brandt Boe-Hansen
Department of Large Animal Sciences, Section for Veterinary Reproduction and Obstetrics, The Royal Veterinary and Agricultural University, Frederiksberg C, Denmark
Hum Reprod 21:1576-82. 2006..The objective was to evaluate the sperm chromatin structure assay (SCSA) as a diagnostic tool in clinical practice for intrauterine insemination (IUI), in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) treatments...
Variability and laboratory factors affecting the sperm chromatin structure assay in human semenGry B Boe-Hansen
The Royal Veterinary and Agricultural University, Department of Large Animal Sciences, Section for Veterinary Reproduction and Obstetrics, Dyrlaegevej 68, 1870 Frederiksberg C, Denmark
J Androl 26:360-8. 2005..Precise protocol description and standardization of the SCSA are therefore essential to achieve high agreement within and between different laboratories...
Increasing storage time of extended boar semen reduces sperm DNA integrityGry B Boe-Hansen
Department of Large Animal Sciences, Veterinary Reproduction and Obstetrics, The Royal Veterinary and Agricultural University, Dyrlaegevej 68, 1870 Frederiksberg C, Denmark
Theriogenology 63:2006-19. 2005..001) was detected. In conclusion, the SCSA cytogram patterns were consistent for different ejaculates within boars and storage of extended boar semen at 18 degrees C for 72 h significantly decreased the integrity of sperm DNA...
DNA integrity in sexed bull sperm assessed by neutral Comet assay and sperm chromatin structure assayGry B Boe-Hansen
Department of Large Animal Sciences, Veterinary Reproduction and Obstetrics, The Royal Veterinary and Agricultural University, Dyrlaegevej 68, 1870 Frederiksberg C, Copenhagen, Denmark
Theriogenology 63:1789-802. 2005..In the future assessment of sperm DNA integrity may be used to select bulls for sperm sex sorting and optimizing sperm sex sorting procedures...
Tachykinins mediate changes in ion transport in porcine jejunum through release of prostaglandins and neurotransmittersJ E Thorbøll
Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark
Regul Pept 77:105-11. 1998..These results indicate, that SP and NKA regulate ion transport in porcine jejunum, entirely through the release of prostaglandins and enteric neurotransmitters...
Endocrine disrupting compounds: effect of octylphenol on reproduction over three generationsI B Bøgh
Department of Clinical Studies, Section for Reproduction, Royal Veterinary and Agricultural University, 68 Dyrlaegevej, 1870 Frederiksberg C, Denmark
Theriogenology 55:131-50. 2001..Furthermore, possible consequences of exposure to endocrine disrupting compounds for the embryo transfer industry are addressed...
Effects of indomethacin on Salmonella typhimurium- and cholera toxin-induced fluid accumulation in the porcine small intestineM A Unmack
Department of Anatomy and Physiology, Royal Veterinary and Agricultural University, , DK-1870 Frederiksberg C, Denmark
J Vet Med A Physiol Pathol Clin Med 48:153-63. 2001..These results indicate that the porcine jejunal secretory response to ST and CT does not involve prostaglandins although indomethacin has an influence on the luminal release of PGE2 but not of 5-HT...
