P Andersen

Summary

Affiliation: Statens Serum Institut
Country: Denmark

Publications

  1. ncbi request reprint Alteration of epitope recognition pattern in Ag85B and ESAT-6 has a profound influence on vaccine-induced protection against Mycobacterium tuberculosis
    Thomas Bennekov
    Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark
    Eur J Immunol 36:3346-55. 2006
  2. doi request reprint Liposome delivery of Chlamydia muridarum major outer membrane protein primes a Th1 response that protects against genital chlamydial infection in a mouse model
    Jon Hansen
    Chlamydia and Adjuvant Research, Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark
    J Infect Dis 198:758-67. 2008
  3. pmc A structurally informed autotransporter platform for efficient heterologous protein secretion and display
    Wouter S P Jong
    Section Molecular Microbiology, Department of Molecular Cell Biology, Faculty of Earth and Life Sciences, VU University, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands
    Microb Cell Fact 11:85. 2012
  4. pmc Development of a proof of concept immunochromatographic lateral flow assay for point of care diagnosis of Mycobacterium tuberculosis
    Liya Wassie
    Armauer Hansen Research Institute, P O Box 1005, Jimma Road, Addis Ababa, Ethiopia
    BMC Res Notes 6:202. 2013
  5. pmc Parasitic infection may be associated with discordant responses to QuantiFERON and tuberculin skin test in apparently healthy children and adolescents in a tuberculosis endemic setting, Ethiopia
    Liya Wassie
    Armauer Hansen Research Institute, Jimma Road, P O Box 1005 Jimma Road, Addis Ababa, Ethiopia
    BMC Infect Dis 13:265. 2013
  6. doi request reprint The hunt for a global killer
    Peter Andersen
    Saten Serum Institut, Copenhagen, Denmark
    Hum Vaccin Immunother 8:547-53. 2012
  7. ncbi request reprint TB subunit vaccines--putting the pieces together
    Peter Andersen
    Department of Infectious Disease Immunology, Statens Serum Institut, 5 Artillerivej, 2300 Copenhagen, Denmark
    Microbes Infect 7:911-21. 2005
  8. ncbi request reprint Specific immune-based diagnosis of tuberculosis
    P Andersen
    Department of Tuberculosis Immunology, Statens Serum Institut, Copenhagen, Denmark
    Lancet 356:1099-104. 2000
  9. ncbi request reprint The prognosis of latent tuberculosis: can disease be predicted?
    Peter Andersen
    Statens Serum Institut, Department of Infectious Disease Immunology, Artillerivej 5, DK 2300 Copenhagen S, Denmark
    Trends Mol Med 13:175-82. 2007
  10. pmc CD4(+) T-cell subsets that mediate immunological memory to Mycobacterium tuberculosis infection in mice
    P Andersen
    Department of TB Immunology, Statens Serum Institut, Copenhagen, Denmark
    Infect Immun 68:621-9. 2000

Detail Information

Publications88

  1. ncbi request reprint Alteration of epitope recognition pattern in Ag85B and ESAT-6 has a profound influence on vaccine-induced protection against Mycobacterium tuberculosis
    Thomas Bennekov
    Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark
    Eur J Immunol 36:3346-55. 2006
    ....
  2. doi request reprint Liposome delivery of Chlamydia muridarum major outer membrane protein primes a Th1 response that protects against genital chlamydial infection in a mouse model
    Jon Hansen
    Chlamydia and Adjuvant Research, Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark
    J Infect Dis 198:758-67. 2008
    ..A need for Th1-polarizing adjuvants in experimental chlamydia vaccines has been demonstrated, and antigen conformation has also been reported as being important for raising protective immunity...
  3. pmc A structurally informed autotransporter platform for efficient heterologous protein secretion and display
    Wouter S P Jong
    Section Molecular Microbiology, Department of Molecular Cell Biology, Faculty of Earth and Life Sciences, VU University, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands
    Microb Cell Fact 11:85. 2012
    ....
  4. pmc Development of a proof of concept immunochromatographic lateral flow assay for point of care diagnosis of Mycobacterium tuberculosis
    Liya Wassie
    Armauer Hansen Research Institute, P O Box 1005, Jimma Road, Addis Ababa, Ethiopia
    BMC Res Notes 6:202. 2013
    ..Unfortunately none of the serodiagnostic approaches have so far proven useful under field conditions...
  5. pmc Parasitic infection may be associated with discordant responses to QuantiFERON and tuberculin skin test in apparently healthy children and adolescents in a tuberculosis endemic setting, Ethiopia
    Liya Wassie
    Armauer Hansen Research Institute, Jimma Road, P O Box 1005 Jimma Road, Addis Ababa, Ethiopia
    BMC Infect Dis 13:265. 2013
    ..It is clear that the two tests, do not always give concordant results, but the factors leading to this are only partially understood...
  6. doi request reprint The hunt for a global killer
    Peter Andersen
    Saten Serum Institut, Copenhagen, Denmark
    Hum Vaccin Immunother 8:547-53. 2012
    ..Since then I have been driven by scientific curiosity and the prospects of generating results that can be used for products for which there is a real need...
  7. ncbi request reprint TB subunit vaccines--putting the pieces together
    Peter Andersen
    Department of Infectious Disease Immunology, Statens Serum Institut, 5 Artillerivej, 2300 Copenhagen, Denmark
    Microbes Infect 7:911-21. 2005
    ..This requires carefully monitoring aspects as immunodominance, recognition in different populations as well as vaccine manufacturing...
  8. ncbi request reprint Specific immune-based diagnosis of tuberculosis
    P Andersen
    Department of Tuberculosis Immunology, Statens Serum Institut, Copenhagen, Denmark
    Lancet 356:1099-104. 2000
    ..tuberculosis either by blood tests in vitro or skin tests in vivo...
  9. ncbi request reprint The prognosis of latent tuberculosis: can disease be predicted?
    Peter Andersen
    Statens Serum Institut, Department of Infectious Disease Immunology, Artillerivej 5, DK 2300 Copenhagen S, Denmark
    Trends Mol Med 13:175-82. 2007
    ..This might provide an unprecedented advantage for TB control, namely targeted preventive therapy for individuals who are most at risk of developing active contagious TB...
  10. pmc CD4(+) T-cell subsets that mediate immunological memory to Mycobacterium tuberculosis infection in mice
    P Andersen
    Department of TB Immunology, Statens Serum Institut, Copenhagen, Denmark
    Infect Immun 68:621-9. 2000
    ..Taken together, these two lines of evidence support an important role for memory cells which have reverted to a naive phenotype in the long-term protection against M. tuberculosis...
  11. ncbi request reprint Vaccine strategies against latent tuberculosis infection
    Peter Andersen
    Department of Infectious Disease Immunology, Statens Serum Institute, DK 2300 Copenhagen, Denmark
    Trends Microbiol 15:7-13. 2007
    ..These late-stage antigens might have potential as post-exposure vaccines or could form the basis for a multi-stage vaccine strategy, in which they are combined with prophylactic vaccines based on early antigens from replicating bacteria...
  12. ncbi request reprint The success and failure of BCG - implications for a novel tuberculosis vaccine
    Peter Andersen
    Statens Serum Institute, Department of Infectious Disease Immunology, Artillerivej 5, 2300 Copenhagen S, Denmark
    Nat Rev Microbiol 3:656-62. 2005
    ..Here, we discuss the potential mechanisms behind the variation of BCG efficacy and their implications for an improved TB vaccination strategy...
  13. ncbi request reprint TB vaccines: progress and problems
    P Andersen
    Dept of TB Immunology, Statens Seruminstitut, 5 Artillerivej, DK 2300 S, Copenhagen, Denmark
    Trends Immunol 22:160-8. 2001
    ..Several hypotheses that might explain the failure of BCG and the strategies designed to address these shortcomings are discussed...
  14. doi request reprint Detection of bovine tuberculosis in herds with different disease prevalence and influence of paratuberculosis infection on PPDB and ESAT-6/CFP10 specificity
    C Aagaard
    Statens Serum Institut, Department of Infectious Immunology, Artilleirvej 5, 2300 Copenhagen, Denmark
    Prev Vet Med 96:161-9. 2010
    ..In high prevalence herds, PPDB or ESAT-6+CFP10 can be used as antigen whereas in low and medium prevalence herds ESAT-6+CFP10 is the preferred choice...
  15. ncbi request reprint Recall of long-lived immunity to Mycobacterium tuberculosis infection in mice
    P Andersen
    Department of Bacterial Vaccine, Statens Seruminstitut, Copenhagen, Denmark
    J Immunol 154:3359-72. 1995
    ..ESAT-6 was biochemically purified, characterized, and the gene encoding the protein was cloned...
  16. pmc Comparative evaluation of low-molecular-mass proteins from Mycobacterium tuberculosis identifies members of the ESAT-6 family as immunodominant T-cell antigens
    R L Skjøt
    Department of TB Immunology, Statens Serum Institut, Copenhagen, Denmark
    Infect Immun 68:214-20. 2000
    ..The striking immunodominance of antigens within the ESAT-6 family is discussed, and hypotheses are presented to explain this targeting of the immune response during TB infection...
  17. ncbi request reprint Specific acquired resistance in mice immunized with killed mycobacteria
    E M Agger
    Department of TB Immunology, Statens Serum Institut, Copenhagen, Denmark
    Scand J Immunol 56:443-7. 2002
    ..Moreover, protective antigens can be found in the cell wall, membrane and cytosol of the mycobacterial cell, and hence emphasize the importance of searching for protective antigens in various compartments of the mycobacterial cell...
  18. pmc Identification and characterization of a 29-kilodalton protein from Mycobacterium tuberculosis culture filtrate recognized by mouse memory effector cells
    I Rosenkrands
    Department of TB Immunology, Statens Serum Institut, Copenhagen, Denmark
    Infect Immun 66:2728-35. 1998
    ..Interspecies analysis by immunoblotting and PCR demonstrated that CFP29 is widely distributed in mycobacterial species...
  19. pmc Protection of mice with a tuberculosis subunit vaccine based on a fusion protein of antigen 85b and esat-6
    A Weinrich Olsen
    Department of TB Immunology, Statens Serum Institute, Copenhagen, Denmark
    Infect Immun 69:2773-8. 2001
    ..The vaccine induced efficient immunological memory, which remained stable 30 weeks postvaccination...
  20. ncbi request reprint Human T cell responses to the ESAT-6 antigen from Mycobacterium tuberculosis
    P Ravn
    Department of Tuberculosis Immunology, Statens Serum Institut, 2300 Copenhagen 5, Denmark
    J Infect Dis 179:637-45. 1999
    ..This study demonstrates that ESAT-6 is frequently recognized during early infection and holds potential as a component of a future TB-specific diagnostic reagent...
  21. pmc Purification and characterization of a low-molecular-mass T-cell antigen secreted by Mycobacterium tuberculosis
    A L Sørensen
    Mycobacteria Department, Statens Seruminstitut, Copenhagen, Denmark
    Infect Immun 63:1710-7. 1995
    ..tuberculosis complex species. Among filtrates from mycobacteria not belonging to the M. tuberculosis complex, reactivity was observed in Mycobacterium kansasii, Mycobacterium szulgai, and Mycobacterium marinum...
  22. pmc Combination of the cationic surfactant dimethyl dioctadecyl ammonium bromide and synthetic mycobacterial cord factor as an efficient adjuvant for tuberculosis subunit vaccines
    L Holten-Andersen
    Department of Infectious Disease Immunology, Statens Serum Institut, DK 2300 Copenhagen, Denmark
    Infect Immun 72:1608-17. 2004
    ..tuberculosis infection in a vaccination model and thus appears to be a very promising candidate for the development of human vaccines...
  23. ncbi request reprint Performance of whole blood IFN-gamma test for tuberculosis diagnosis based on PPD or the specific antigens ESAT-6 and CFP-10
    I Brock
    Department of Tuberculosis Immunology, Statens Serum Institute, Copenhagen, Denmark
    Int J Tuberc Lung Dis 5:462-7. 2001
    ..Such a test would provide a quantum improvement over the current practice of using the tuberculin skin test for TB control and elimination...
  24. ncbi request reprint Tuberculosis subunit vaccine development: on the role of interferon-gamma
    E M Agger
    Department of TB Immunology, Statens Serum Institute, Artillerivej 5, 2300 S, Copenhagen, Denmark
    Vaccine 19:2298-302. 2001
    ....
  25. ncbi request reprint Antigen discovery and tuberculosis vaccine development in the post-genomic era
    R Louise
    Department of TB Immunology, Statens Serum Institut, Copenhagen, Denmark
    Scand J Infect Dis 33:643-7. 2001
    ..The data accumulated so far demonstrate that these molecules are immunodominant antigens strongly recognized in human TB patients and with the potential for a novel TB vaccine...
  26. pmc Recognition of ESAT-6 sequences by antibodies in sera of tuberculous nonhuman primates
    G V Kanaujia
    Public Health Research Institute, Newark, New Jersey Merck Research Laboratories, West Point, Pennsylvania Department of Comparative Medicine, Stanford University, Stanford, California Statens Serum Institute, Copenhagen, Denmark
    Clin Diagn Lab Immunol 11:222-6. 2004
    ..The data support use of synthetic peptides in lieu of the full-length ESAT-6 protein in diagnostic antibody detection assays...
  27. ncbi request reprint Development of the Mycobacterium bovis BCG vaccine: review of the historical and biochemical evidence for a genealogical tree
    T Oettinger
    Department of TB Immunology, Statens Serum Institut, Copenhagen, Denmark
    Tuber Lung Dis 79:243-50. 1999
    ..Further evidence from the literature is provided that a change occurred in virulence of the BCG parent strain at Institut Pasteur in the late 1920s. Based on this information a genealogical tree is proposed and discussed...
  28. ncbi request reprint Towards the proteome of Mycobacterium tuberculosis
    I Rosenkrands
    Department of TB Immunology, Statens Serum Institute, Copenhagen, Denmark
    Electrophoresis 21:3740-56. 2000
    ..ssi.dk/publichealth/tbimmun). The information obtained from the M. tuberculosis proteome so far is discussed in relation to the information obtained from the complete genome sequence...
  29. ncbi request reprint A novel TB vaccine; towards a strategy based on our understanding of BCG failure
    E M Agger
    Department of Infectious Disease Immunology, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen S, Denmark
    Vaccine 21:7-14. 2002
    ....
  30. pmc Optimizing antigen cocktails for detection of Mycobacterium bovis in herds with different prevalences of bovine tuberculosis: ESAT6-CFP10 mixture shows optimal sensitivity and specificity
    C Aagaard
    Statens Serum Institut, Department of TB Immunology, Copenhagen, Denmark
    J Clin Microbiol 44:4326-35. 2006
    ..Furthermore, TB10.4, PE13, and PE5 have the potential to supplement ESAT6 and CFP10 in a future five-component diagnostic cocktail...
  31. ncbi request reprint Evaluation of a new commercial assay for diagnosis of pulmonary and nonpulmonary tuberculosis
    I S Johansen
    International Reference Laboratory of Mycobacteriology, Statens Serum Institut, 5 Artillerivej, 2300 Copenhagen, Denmark
    Eur J Clin Microbiol Infect Dis 21:455-60. 2002
    ..The BDProbeTec ET assay is a fast, effective, and user-friendly system that can be used for rapid detection of MTC bacteria in respiratory and microscopy-positive nonrespiratory specimens as an important supplement to smear and culture...
  32. pmc Structure and function of a 40,000-molecular-weight protein antigen of Mycobacterium tuberculosis
    A B Andersen
    Mycobacteria Department, Statens Seruminstitut, Copenhagen, Denmark
    Infect Immun 60:2317-23. 1992
    ..The enzyme was characterized and purified by chromatography, thus enabling further studies of its role in virulence and interaction with the immune system of M. tuberculosis-infected individuals...
  33. pmc The combined CTA1-DD/ISCOMs vector is an effective intranasal adjuvant for boosting prior Mycobacterium bovis BCG immunity to Mycobacterium tuberculosis
    Claire Swetman Andersen
    Staten Serum Institute, TB Vaccine Research, Department of Infectious Disease Immunology, 5 Artillerivej, DK 2300 Copenhagen S, Denmark
    Infect Immun 75:408-16. 2007
    ..Thus, the results demonstrate the effectiveness of mucosal vaccination with Ag85B-ESAT-6 mixed with CTA1-DD/ISCOMs as adjuvant for stimulating TB-specific protective immunity in the lung...
  34. ncbi request reprint Mucosal administration of Ag85B-ESAT-6 protects against infection with Mycobacterium tuberculosis and boosts prior bacillus Calmette-Guerin immunity
    Jes Dietrich
    Department of Infectious Disease Immunology, Statens Serum Institute, Artillerivej 5, DK 2300 Copenhagen, Denmark
    J Immunol 177:6353-60. 2006
    ..tb in LTK63/Ag85B-ESAT-6-boosted mice, compared with BCG-vaccinated animals. Thus, LTK63/Ag85B-ESAT-6 represents an efficient preventive vaccine against tuberculosis with a strong ability to boost prior BCG immunity...
  35. ncbi request reprint Identification of CT521 as a frequent target of Th1 cells in patients with urogenital Chlamydia trachomatis infection
    Anja Weinreich Olsen
    Chlamydia Research, Department of Infectious Disease Immunology, Statens Serum Institute, DK 2300 Copenhagen S, Denmark
    J Infect Dis 194:1258-66. 2006
    ..The human immune response to a Chlamydia trachomatis serovar D lysate was investigated in patients with urogenital C. trachomatis infection, to identify novel T cell targets...
  36. ncbi request reprint Novel overlapping coding sequences in Chlamydia trachomatis
    Klaus T Jensen
    Laboratory of Infectious Diseases Immunology, Chlamydia Vaccine Unit, Statens Serum Institut, 5 Artillerivej, DK 2300 Copenhagen, Denmark
    FEMS Microbiol Lett 265:106-17. 2006
    ..The combined evidence suggests that at least seven of the 15 are protein coding genes. The data suggest the presence of overlapping active genes in C. trachomatis...
  37. ncbi request reprint Human T-cell responses to 25 novel antigens encoded by genes of the dormancy regulon of Mycobacterium tuberculosis
    Eliane M S Leyten
    Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    Microbes Infect 8:2052-60. 2006
    ..Particularly strong IFN-gamma responses to latency antigens were observed in latently infected individuals, suggesting that immune responses against these antigens may contribute to controlling latent M. tuberculosis infection...
  38. ncbi request reprint Cytokine profiles in tuberculosis patients and healthy subjects in response to complex and single antigens of Mycobacterium tuberculosis
    Raja a Al-Attiyah
    Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait
    FEMS Immunol Med Microbiol 47:254-61. 2006
    ..The secretion of IFN-gamma, but not IL-10, in the presence of Ag85B, ESAT6 and MPT64 supports their potential as subunit vaccine candidates against TB...
  39. pmc Tuberculosis in elephants: antibody responses to defined antigens of Mycobacterium tuberculosis, potential for early diagnosis, and monitoring of treatment
    Konstantin P Lyashchenko
    Chembio Diagnostic Systems, Inc, 3661 Horseblock Road, Medford, New York 11763, USA
    Clin Vaccine Immunol 13:722-32. 2006
    ..Similarly to MAPIA, infected elephants were identified using the RT up to 4 years prior to positive culture. These findings demonstrate the potential for TB surveillance and treatment monitoring using the RT and MAPIA, respectively...
  40. ncbi request reprint A comparative study of cationic liposome and niosome-based adjuvant systems for protein subunit vaccines: characterisation, environmental scanning electron microscopy and immunisation studies in mice
    Anil Vangala
    Medicines Research Unit, School of Life and Health Sciences, Aston University, Birmingham, B4 7ET, UK
    J Pharm Pharmacol 58:787-99. 2006
    ..The MP- and DDA-based systems induced antibody responses at comparable levels whereas the DDA-based systems induced more powerful cell-mediated immune responses...
  41. ncbi request reprint Trehalose preserves DDA/TDB liposomes and their adjuvant effect during freeze-drying
    Dennis Christensen
    Statens Serum Institut, Department of Infectious Disease Immunology, Adjuvant Research, Artillerivej 5, DK 2300 Copenhagen S, Denmark
    Biochim Biophys Acta 1768:2120-9. 2007
    ....
  42. ncbi request reprint Tuberculosis vaccines - an update
    Peter Andersen
    Nat Rev Microbiol 5:484-7. 2007
  43. pmc ESAT-6/CFP10 skin test predicts disease in M. tuberculosis-infected guinea pigs
    Karin Weldingh
    Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark
    PLoS ONE 3:e1978. 2008
    ..We have studied the ability of a skin test response to ESAT-6 and CFP10 (E6/C10) to predict later development of tuberculosis disease in the guinea pig model...
  44. doi request reprint Protective anti-mycobacterial T cell responses through exquisite in vivo activation of vaccine-targeted dendritic cells
    Arun T Kamath
    World Health Organization Collaborating Center for Vaccinology and Neonatal Immunology, Departments of Pathology Immunology and Pediatrics, University of Geneva, Geneva, Switzerland
    Eur J Immunol 38:1247-56. 2008
    ..Thus, potent protective IFN-gamma-producing responses may be elicited by the exquisite activation of a minute number of in vivo targeted DC...
  45. doi request reprint Antigenic profiling of a Chlamydia trachomatis gene-expression library
    Frank Follmann
    Chlamydia Research, Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark
    J Infect Dis 197:897-905. 2008
    ..0013). Overall, we have identified 5 T cell antigens, 5 B cell antigens, and 2 T/B cell antigens that are potential components for a future chlamydia vaccine...
  46. pmc Adjuvant modulation of the cytokine balance in Mycobacterium tuberculosis subunit vaccines; immunity, pathology and protection
    Else Marie Agger
    Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark
    Immunology 124:175-85. 2008
    ..This study indicates that the adjuvant component of a tuberculosis vaccine may be crucial in determining the kinetics by which effective granulomas, pivotal in controlling bacterial growth, are formed...
  47. ncbi request reprint Double-blind randomized Phase I study comparing rdESAT-6 to tuberculin as skin test reagent in the diagnosis of tuberculosis infection
    Sandra M Arend
    Department of Infectious Diseases, C5P, Leiden University Medical Center LUMC, P O Box 9600, 2300 RC Leiden, The Netherlands
    Tuberculosis (Edinb) 88:249-61. 2008
    ..1 microg. Further studies are needed to evaluate sensitization after repeated doses and to study the effect of additional CFP-10 on the sensitivity of a TB-specific skin test...
  48. ncbi request reprint Identification of human T cell targets recognized during Chlamydia trachomatis genital infection
    Anja Weinreich Olsen
    Chlamydia Research Group, Department of Infectious Disease Immunology, Statens Serum Institut, Artillerivej 5, Copenhagen, Denmark
    J Infect Dis 196:1546-52. 2007
    ..McDonald-Kreitman and Tajima's D tests involving clinical isolates from the same samples showed evidence for frequency-dependent selection on ct583. We predict that CT583 is a target of acquired protective immune responses in humans...
  49. doi request reprint alpha,alpha'-trehalose 6,6'-dibehenate in non-phospholipid-based liposomes enables direct interaction with trehalose, offering stability during freeze-drying
    Dennis Christensen
    Statens Serum Institut, Department of Infectious Disease Immunology, Adjuvant Research, DK 2300 Copenhagen S, Denmark
    Biochim Biophys Acta 1778:1365-73. 2008
    ..Furthermore the results indicate that direct interaction with liposome surfaces is necessary for trehalose to enable protection during freeze-drying...
  50. ncbi request reprint Cationic liposomes as vaccine adjuvants
    Dennis Christensen
    Statens Serum Institut, Department of Infectious Disease Immunology, Artillerivej 5, 81 306, DK 2300 Copenhagen S, Copenhagen, Denmark
    Expert Rev Vaccines 6:785-96. 2007
    ..In this article we review the recent progress on cationic liposomes as vehicles, enhancing the effect of immunomodulators and the presentation of vaccine antigens...
  51. ncbi request reprint Induction of CD8 T cells against a novel epitope in TB10.4: correlation with mycobacterial virulence and the presence of a functional region of difference-1
    Rolf Billeskov
    Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark
    J Immunol 179:3973-81. 2007
    ..4(3-11)-specific CD8 T cells, and exhibited increased virulence compared with BCG, our data suggest that virulence in itself is also involved in generating a robust CD8 T cell response against mycobacterial epitopes, such as TB10.4(3-11)...
  52. ncbi request reprint Evaluation of Mycobacterium tuberculosis--specific antibody responses in populations with different levels of exposure from Tanzania, Ethiopia, Brazil, and Denmark
    Soren T Hoff
    Department of Infectious Diseases Immunology, Statens Serum Institute, Copenhagen S, Denmark
    Clin Infect Dis 45:575-82. 2007
    ..A key question remaining is the extent to which the disappointing performance of current tests is associated with a high background prevalence of latent TB...
  53. pmc PrimaTB STAT-PAK assay, a novel, rapid lateral-flow test for tuberculosis in nonhuman primates
    Konstantin P Lyashchenko
    Chembio Diagnostic Systems, Inc, 3661 Horseblock Road, Medford, NY 11763, USA
    Clin Vaccine Immunol 14:1158-64. 2007
    ..The highest rate of TB detection was achieved when the skin test was combined with the PrimaTB STAT-PAK kit. This novel immunoassay provides a simple, rapid, and accurate test for TB in NHP...
  54. ncbi request reprint Detecting a low prevalence of latent tuberculosis among health care workers in Denmark detected by M. tuberculosis specific IFN-gamma whole-blood test
    Bolette Soborg
    International Reference Laboratory of Mycobacteriology, Statens Serum Institute, Copenhagen, Denmark
    Scand J Infect Dis 39:554-9. 2007
    ..The QFT-TB may be useful in distinguishing persons with latent TB infection from persons with positive TST due to BCG vaccination and its use may reduce anxiety...
  55. ncbi request reprint TB vaccine strategies--what is needed to solve a complex problem?
    Jes Dietrich
    Department of Infectious Disease Immunology, Statens Serum Institute, Copenhagen, Denmark
    Tuberculosis (Edinb) 86:163-8. 2006
    ..In this review we will discuss some of the most advanced attempts to address this challenge...
  56. ncbi request reprint Protective immunity to tuberculosis with Ag85B-ESAT-6 in a synthetic cationic adjuvant system IC31
    Else Marie Agger
    Department of Infectious Disease Immunology, Statens Serum Institut, Adjuvant Research, 5 Artillerivej, DK 2300 Copenhagen S, Denmark
    Vaccine 24:5452-60. 2006
    ..Furthermore, the combination of Ag85B-ESAT-6/IC31 was found to confer efficient protection in the guinea pig aerosol model of tuberculosis infection and is at present moving towards clinical testing...
  57. ncbi request reprint Characterization of a Mycobacterium tuberculosis peptide that is recognized by human CD4+ and CD8+ T cells in the context of multiple HLA alleles
    Homayoun Shams
    Center for Pulmonary and Infectious Disease Control, and Department of Microbiology, University of Texas Health Center, Tyler, TX 75708, USA
    J Immunol 173:1966-77. 2004
    ....
  58. pmc Specific T-cell epitopes for immunoassay-based diagnosis of Mycobacterium tuberculosis infection
    Inger Brock
    Department of Infectious Disease Immunology, Statens Serum Institute, Artillerivej 5, DK 2300 Copenhagen S, Denmark
    J Clin Microbiol 42:2379-87. 2004
    ..In a validation experiment with new experimental groups, the sensitivities obtained were 57% for the combination of peptides and 90% for the combination of the peptides, ESAT-6, and CFP-10. This combination gave a specificity of 95%...
  59. pmc Association of tuberculin-boosted antibody responses with pathology and cell-mediated immunity in cattle vaccinated with Mycobacterium bovis BCG and infected with M. bovis
    Konstantin Lyashchenko
    Chembio Diagnostic Systems, Inc, Medford, New York, USA
    Infect Immun 72:2462-7. 2004
    ....
  60. ncbi request reprint Comparison of tuberculin skin test and new specific blood test in tuberculosis contacts
    Inger Brock
    Department of Infectious Disease Immunology, Statens Serum Institut, Artillerivej 5, DK 2300 Copenhagen S, Denmark
    Am J Respir Crit Care Med 170:65-9. 2004
    ..An excellent agreement between the two tests was found (94%, kappa value 0.866), and in contrast to the blood test based on PPD, the novel blood test was not influenced by the vaccination status of the subjects tested...
  61. ncbi request reprint Mapping immune reactivity toward Rv2653 and Rv2654: two novel low-molecular-mass antigens found specifically in the Mycobacterium tuberculosis complex
    Claus Aagaard
    Department of Infectious Disease Immunology, Statens Serum Institute, Copenhagen, Denmark
    J Infect Dis 189:812-9. 2004
    ..Rv2654 is a strongly recognized T cell antigen that is highly specific for TB and has potential as a novel cell-mediated immunity-based TB diagnostic agent...
  62. pmc PPE protein (Rv3873) from DNA segment RD1 of Mycobacterium tuberculosis: strong recognition of both specific T-cell epitopes and epitopes conserved within the PPE family
    Limei Meng Okkels
    Department of Infectious Disease Immunology, Statens Serum Institut, DK 2300 Copenhagen, Denmark
    Infect Immun 71:6116-23. 2003
    ..The immunodominant epitope (residues 118 to 135) for both TB patients and BCG-vaccinated individuals was found to be highly conserved among a large number of PPE family members...
  63. pmc Genomic approach to identification of Mycobacterium bovis diagnostic antigens in cattle
    Claus Aagaard
    Department of Infectious Disease Immunology, Statens Serum Institute, Copenhagen, Denmark
    J Clin Microbiol 41:3719-28. 2003
    ..4 responsiveness. The results suggest that combinations of specific antigens such as these have great potential in development of optimized diagnostic systems for bovine tuberculosis...
  64. ncbi request reprint Improved serodetection of Mycobacterium bovis infection in badgers (Meles meles) using multiantigen test formats
    Rena Greenwald
    Chembio Diagnostic Systems, Inc, Medford, NY, USA
    Diagn Microbiol Infect Dis 46:197-203. 2003
    ..This is the first report of a diagnostic test for badger tuberculosis that can be performed alongside the captive animal...
  65. pmc Gamma interferon responses induced by a panel of recombinant and purified mycobacterial antigens in healthy, non-mycobacterium bovis BCG-vaccinated Malawian young adults
    Gillian F Black
    Karonga Prevention Study, Chilumba, Malawi
    Clin Diagn Lab Immunol 10:602-11. 2003
    ..tuberculosis antigens tested it provided the best indication of exposure to, or infection with, M. tuberculosis...
  66. ncbi request reprint Comparison of T-cell-based assay with tuberculin skin test for diagnosis of Mycobacterium tuberculosis infection in a school tuberculosis outbreak
    Katie Ewer
    Nuffield Department of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK
    Lancet 361:1168-73. 2003
    ..We postulated that if the ELISPOT is a more accurate test of latent infection than TST, it should correlate better with degree of exposure to M tuberculosis...
  67. pmc Responses of bovine WC1(+) gammadelta T cells to protein and nonprotein antigens of Mycobacterium bovis
    Michael D Welsh
    Veterinary Sciences Division, The Department of Agriculture and Rural Development, Queen s University of Belfast, Stormont, Belfast BT4 3SD, United Kingdom
    Infect Immun 70:6114-20. 2002
    ..bovis infection, with nonprotein antigens inducing significant proliferation. These findings have important implications for diagnostic and vaccine development...
  68. pmc Epitope mapping of the immunodominant antigen TB10.4 and the two homologous proteins TB10.3 and TB12.9, which constitute a subfamily of the esat-6 gene family
    Rikke Louise Vinther Skjøt
    Department of TB Immunology, Statens Serum Institute, Copenhagen, Denmark
    Infect Immun 70:5446-53. 2002
    ..4 stimulated the largest responses, but epitopes unique to both TB10.3 and TB12.9 were found. The relevance of the findings for TB vaccine development and as a potential mechanism for immune evasion is discussed...
  69. pmc Oral vaccination with subunit vaccines protects animals against aerosol infection with Mycobacterium tuberculosis
    T Mark Doherty
    Department of Tuberculosis Immunology, Statens Serum Institute, Copenhagen, Denmark
    Infect Immun 70:3111-21. 2002
    ....
  70. ncbi request reprint Tuberculosis vaccine development
    T Mark Doherty
    Department of Tuberculosis Immunology, Statens Serum Institut, Copenhagen, Denmark
    Curr Opin Pulm Med 8:183-7. 2002
    ..This selection is being eased by the improved animal models that have been developed over the past few years and by our increasing understanding of immunity to TB...
  71. ncbi request reprint Reactivation of tuberculosis during immunosuppressive treatment in a patient with a positive QuantiFERON-RD1 test
    Pernille Ravn
    Department of Infectious Diseases, Hvidovre Hospital, Copenhagen, Denmark
    Scand J Infect Dis 36:499-501. 2004
    ..We suggest that RD1 based tests are evaluated further in immunocompromised patients...
  72. pmc Protective effect of a tuberculosis subunit vaccine based on a fusion of antigen 85B and ESAT-6 in the aerosol guinea pig model
    Anja W Olsen
    Department of Infectious Disease Immunology, Statens Serum Institute, Copenhagen, Denmark
    Infect Immun 72:6148-50. 2004
    ..The protection was manifested as delayed clinical illness and prolonged survival. Neither Ag85B nor ESAT-6 (independently or as a cocktail) induced significant protection in this model...
  73. ncbi request reprint Comparative analysis of different vaccine constructs expressing defined antigens from Mycobacterium tuberculosis
    T Mark Doherty
    Department of Infectious Disease Immunology, Statens Seruminstitut, Copenhagen, Denmark
    J Infect Dis 190:2146-53. 2004
    ....
  74. pmc Recognition of stage-specific mycobacterial antigens differentiates between acute and latent infections with Mycobacterium tuberculosis
    Abebech Demissie
    Armauer Hansen Research Institute, Addis Ababa, Ethiopia
    Clin Vaccine Immunol 13:179-86. 2006
    ....
  75. ncbi request reprint Prospects for a novel vaccine against tuberculosis
    Jes Dietrich
    Department of Infectious Disease Immunology, Statens Serum Institute, Artillerivej 5, 2300 Copenhagen S, Denmark
    Vet Microbiol 112:163-9. 2006
    ....
  76. ncbi request reprint Characterization of cationic liposomes based on dimethyldioctadecylammonium and synthetic cord factor from M. tuberculosis (trehalose 6,6'-dibehenate)-a novel adjuvant inducing both strong CMI and antibody responses
    Jesper Davidsen
    Vaccine Development, Adjuvant Research, Statens Serum Institut, DK 2300 Copenhagen, Denmark
    Biochim Biophys Acta 1718:22-31. 2005
    ..The lymphocyte subset releasing the interferon-gamma was identified as CD4 T cells...
  77. pmc Vaccines for tuberculosis: novel concepts and recent progress
    T Mark Doherty
    Department of Infectious Disease Immunology, Statens Serum Institute, Copenhagen, Denmark
    Clin Microbiol Rev 18:687-702. 2005
    ..This provides a background for describing the new generation of vaccines designed to supplement or replace the current vaccine and the different approaches they take to stimulate immunity against M. tuberculosis...
  78. ncbi request reprint [Replacing the tuberculin skin test with a specific blood test]
    Karin Weldingh
    Department of Infectious Disease Immunology, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark
    Kekkaku 80:581-5. 2005
    ..tuberculosis specific antigens ESAT6 and CFP10; two low molecular weight proteins which are highly sensitive and specific for detection of a M. tuberculosis infection...
  79. pmc Cationic liposomes containing mycobacterial lipids: a new powerful Th1 adjuvant system
    Ida Rosenkrands
    Statens Serum Institut, Department of Infectious Disease Immunology, Adjuvant Research, 5 Artillerivej, DK 2300 Copenhagen S, Denmark
    Infect Immun 73:5817-26. 2005
    ..In a tuberculosis challenge model, the mycosomes combined with the Ag85B-ESAT-6 fusion protein were demonstrated to have a unique ability to maintain sustained immunological memory at a level superior to live BCG...
  80. ncbi request reprint [A possible successor of the Mantoux test after 97 years]
    Pernille Ravn
    Ugeskr Laeger 167:2905-6. 2005
  81. ncbi request reprint Exchanging ESAT6 with TB10.4 in an Ag85B fusion molecule-based tuberculosis subunit vaccine: efficient protection and ESAT6-based sensitive monitoring of vaccine efficacy
    Jes Dietrich
    Department of Infectious Disease Immunology, Statens Serum Institute, Copenhagen, Denmark
    J Immunol 174:6332-9. 2005
    ..4, we show that ESAT-6, apart from being an excellent diagnostic reagent, can also be used as a reagent for monitoring vaccine efficacy. This may open a new way for monitoring vaccine efficacy in clinical trials...
  82. ncbi request reprint Healthy individuals that control a latent infection with Mycobacterium tuberculosis express high levels of Th1 cytokines and the IL-4 antagonist IL-4delta2
    Abebech Demissie
    Armauer Hansen Research Institute, Addis Ababa, Ethiopia
    J Immunol 172:6938-43. 2004
    ..These data suggest that long-term control of M. tuberculosis infection is associated not just with elevated Th1 responses but also with inhibition of the Th2 response...
  83. ncbi request reprint Effect of sample handling on analysis of cytokine responses to Mycobacterium tuberculosis in clinical samples using ELISA, ELISPOT and quantitative PCR
    T Mark Doherty
    Department of Tuberculosis Immunology, Statens Serum Institute, Copenhagen, Denmark
    J Immunol Methods 298:129-41. 2005
    ..Researchers should carefully consider these factors when planning human field studies...
  84. pmc Prospective evaluation of a whole-blood test using Mycobacterium tuberculosis-specific antigens ESAT-6 and CFP-10 for diagnosis of active tuberculosis
    Pernille Ravn
    Department of Infectious Diseases, Hvidovre Hospital, Kettegårds Alle 30, 2650 Hvidovre, Copenhagen, Denmark
    Clin Diagn Lab Immunol 12:491-6. 2005
    ..The accuracy of the QFT-RD1 test will vary with the prevalence of LTBI. We suggest that the QFT-RD1 test could be a very useful supplementary tool for the diagnosis of TB...
  85. ncbi request reprint Protection of macaques against Mycobacterium tuberculosis infection by a subunit vaccine based on a fusion protein of antigen 85B and ESAT-6
    Jan A M Langermans
    Department of Parasitology, Biomedical Primate Research Centre, P O Box 3306, 2280 GH Rijswijk, The Netherlands
    Vaccine 23:2740-50. 2005
    ..As far as we are aware this is the first report of protection in primates with a subunit vaccine...
  86. ncbi request reprint Evaluation of vaccines in the EU TB Vaccine Cluster using a guinea pig aerosol infection model of tuberculosis
    Ann Williams
    Health Protection Agency, Porton Down, Salisbury SP4 OJG, UK
    Tuberculosis (Edinb) 85:29-38. 2005
    ..A relatively high aerosol-challenge dose and evaluation over a protracted time post-challenge allowed superior protection over BCG to be demonstrated by BCG boosted with MVA and fowl pox vectors expressing Ag85A...
  87. pmc Assessing the serodiagnostic potential of 35 Mycobacterium tuberculosis proteins and identification of four novel serological antigens
    Karin Weldingh
    Department of Infectious Disease Immunology, Statens Serum Institut, Artillerivej 5, DK 2300 Copenhagen S, Denmark
    J Clin Microbiol 43:57-65. 2005
    ..3 and the TB9.7 antigens were recognized by more than 85% of the samples from TB patients coinfected with human immunodeficiency virus, a patient group for which it is in general difficult to detect M. tuberculosis-specific antibodies...
  88. pmc Failure of the Mycobacterium bovis BCG vaccine: some species of environmental mycobacteria block multiplication of BCG and induction of protective immunity to tuberculosis
    Lise Brandt
    Department of TB Immunology, Statens Serum Institut, Copenhagen, Denmark
    Infect Immun 70:672-8. 2002
    ....