B Munch-Petersen

Summary

Affiliation: Roskilde University
Country: Denmark

Publications

  1. doi request reprint Enzymatic regulation of cytosolic thymidine kinase 1 and mitochondrial thymidine kinase 2: a mini review
    B Munch-Petersen
    Department of Science, Systems and Models NSM, Roskilde, Denmark
    Nucleosides Nucleotides Nucleic Acids 29:363-9. 2010
  2. ncbi request reprint Functional expression of a multisubstrate deoxyribonucleoside kinase from Drosophila melanogaster and its C-terminal deletion mutants
    B Munch-Petersen
    Department of Life Sciences and Chemistry, Roskilde University, P O Box 260, DK 4000 Roskilde, Denmark
    J Biol Chem 275:6673-9. 2000
  3. doi request reprint Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration
    Birgitte Munch-Petersen
    Department of Science, Systems and Models, Roskilde University, Denmark
    FEBS J 276:571-80. 2009
  4. ncbi request reprint Four deoxynucleoside kinase activities from Drosophila melanogaster are contained within a single monomeric enzyme, a new multifunctional deoxynucleoside kinase
    B Munch-Petersen
    Department of Life Sciences and Chemistry, Roskilde University, P O Box 260, DK 4000 Roskilde, Denmark
    J Biol Chem 273:3926-31. 1998
  5. ncbi request reprint Valine, not methionine, is amino acid 106 in human cytosolic thymidine kinase (TK1). Impact on oligomerization, stability, and kinetic properties
    D Berenstein
    Department of Life Sciences and Chemistry, Roskilde University, DK 4000 Roskilde, Denmark
    J Biol Chem 275:32187-92. 2000
  6. doi request reprint Thymidine kinase 1 deficient cells show increased survival rate after UV-induced DNA damage
    T Skovgaard
    Department of Science Systems and Models, Roskilde University, Roskilde, Denmark
    Nucleosides Nucleotides Nucleic Acids 29:347-51. 2010
  7. ncbi request reprint Thymidine in the micromolar range promotes rejoining of UVC-induced DNA strand breaks and prevents azidothymidine from inhibiting the rejoining in quiescent human lymphocytes
    B Munch-Petersen
    Department of Life Sciences and Chemistry, Roskilde University, Denmark
    Mutat Res 383:143-53. 1997
  8. ncbi request reprint Effect of C-terminal of human cytosolic thymidine kinase (TK1) on in vitro stability and enzymatic properties
    C Zhu
    Department of Life Sciences and Chemistry, Roskilde University, Roskilde, Denmark
    Nucleosides Nucleotides Nucleic Acids 25:1185-8. 2006
  9. ncbi request reprint The role of mitochondrial dNTP levels in cells with reduced TK2 activity
    C Desler
    Department of Life Sciences and Chemistry, Roskilde University, Roskilde, Denmark
    Nucleosides Nucleotides Nucleic Acids 25:1171-5. 2006
  10. ncbi request reprint Purification and characterization of wild-type and mutant TK1 type kinases from Caenorhabditis elegans
    T Skovgaard
    Department of Life Sciences and Chemistry, Roskilde University, Roskilde, Denmark
    Nucleosides Nucleotides Nucleic Acids 25:1165-9. 2006

Collaborators

  • L J Rasmussen
  • J Piskur
  • C Zhu
  • Lusheng Wang
  • T Skovgaard
  • W Knecht
  • C Desler
  • K Johansson
  • D Berenstein
  • S Eriksson
  • L Hasholt
  • M Willer
  • Z Gojkovic
  • E Rozpedowska
  • C Le Breton
  • T Joergensen
  • M P B Sandrini
  • B Kierdaszuk
  • S Ramaswamy
  • H Eklund
  • C Ljungcrantz
  • T Kristensen
  • R Hofbauer
  • J F Christensen
  • N G Johansson
  • K Krawiec
  • Z Kazimierczuk
  • U Jacobsson
  • D Shugar

Detail Information

Publications16

  1. doi request reprint Enzymatic regulation of cytosolic thymidine kinase 1 and mitochondrial thymidine kinase 2: a mini review
    B Munch-Petersen
    Department of Science, Systems and Models NSM, Roskilde, Denmark
    Nucleosides Nucleotides Nucleic Acids 29:363-9. 2010
    ..The complex enzymatic regulation of TK1 and TK2 and the possible physiological significance of this regulation will be discussed...
  2. ncbi request reprint Functional expression of a multisubstrate deoxyribonucleoside kinase from Drosophila melanogaster and its C-terminal deletion mutants
    B Munch-Petersen
    Department of Life Sciences and Chemistry, Roskilde University, P O Box 260, DK 4000 Roskilde, Denmark
    J Biol Chem 275:6673-9. 2000
    ..Removal of the C-terminal 20 amino acids increased the specific activity 2-fold, but 99% of the activity was lost after removal of the C-terminal 30 amino acids...
  3. doi request reprint Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration
    Birgitte Munch-Petersen
    Department of Science, Systems and Models, Roskilde University, Denmark
    FEBS J 276:571-80. 2009
    ..Furthermore, the enzyme concentration was found to have a pivotal effect on catalytic efficiency...
  4. ncbi request reprint Four deoxynucleoside kinase activities from Drosophila melanogaster are contained within a single monomeric enzyme, a new multifunctional deoxynucleoside kinase
    B Munch-Petersen
    Department of Life Sciences and Chemistry, Roskilde University, P O Box 260, DK 4000 Roskilde, Denmark
    J Biol Chem 273:3926-31. 1998
    ....
  5. ncbi request reprint Valine, not methionine, is amino acid 106 in human cytosolic thymidine kinase (TK1). Impact on oligomerization, stability, and kinetic properties
    D Berenstein
    Department of Life Sciences and Chemistry, Roskilde University, DK 4000 Roskilde, Denmark
    J Biol Chem 275:32187-92. 2000
    ....
  6. doi request reprint Thymidine kinase 1 deficient cells show increased survival rate after UV-induced DNA damage
    T Skovgaard
    Department of Science Systems and Models, Roskilde University, Roskilde, Denmark
    Nucleosides Nucleotides Nucleic Acids 29:347-51. 2010
    ..Our results suggest that higher thymidine levels in the TK- cells caused by defect thymidine salvage to dTTP protects against UV irradiation...
  7. ncbi request reprint Thymidine in the micromolar range promotes rejoining of UVC-induced DNA strand breaks and prevents azidothymidine from inhibiting the rejoining in quiescent human lymphocytes
    B Munch-Petersen
    Department of Life Sciences and Chemistry, Roskilde University, Denmark
    Mutat Res 383:143-53. 1997
    ..The results also indicate that an expansion of the dTTP pool may counteract the inhibiting effect of AZT on DNA repair in quiescent lymphocytes...
  8. ncbi request reprint Effect of C-terminal of human cytosolic thymidine kinase (TK1) on in vitro stability and enzymatic properties
    C Zhu
    Department of Life Sciences and Chemistry, Roskilde University, Roskilde, Denmark
    Nucleosides Nucleotides Nucleic Acids 25:1185-8. 2006
    ..We found that deletion of the C-terminal fold markedly increased the stability as well as the catalytic activity...
  9. ncbi request reprint The role of mitochondrial dNTP levels in cells with reduced TK2 activity
    C Desler
    Department of Life Sciences and Chemistry, Roskilde University, Roskilde, Denmark
    Nucleosides Nucleotides Nucleic Acids 25:1171-5. 2006
    ..Our results suggest that import of cytosolic dNTPs in mitochondria of proliferating cells can compensate a TK2 induced imbalance of the mitochondrial dNTP pool...
  10. ncbi request reprint Purification and characterization of wild-type and mutant TK1 type kinases from Caenorhabditis elegans
    T Skovgaard
    Department of Life Sciences and Chemistry, Roskilde University, Roskilde, Denmark
    Nucleosides Nucleotides Nucleic Acids 25:1165-9. 2006
    ..This activation only occurs at an enzyme concentration in the incubation buffer of 0.5 micro g/ml (8.42 nM) or higher. C-terminal deletion of the enzyme results in lower catalytic efficiency and stability...
  11. ncbi request reprint Drosophila deoxyribonucleoside kinase mutants with enhanced ability to phosphorylate purine analogs
    W Knecht
    BioCentrum DTU, Technical University of Denmark, Lyngby, Denmark
    Gene Ther 14:1278-86. 2007
    ..We also show that a choice of the selection and screening system plays a crucial role when optimizing suicide genes by directed evolution...
  12. ncbi request reprint Structural basis for substrate specificities of cellular deoxyribonucleoside kinases
    K Johansson
    Department of Molecular Biology, Swedish University of Agricultural Sciences, Box 590, Biomedical Center, S 751 24 Uppsala, Sweden
    Nat Struct Biol 8:616-20. 2001
    ..The two new structures provide an explanation for the substrate specificity of cellular deoxyribonucleoside kinases...
  13. ncbi request reprint Identification of residues involved in the specificity and regulation of the highly efficient multisubstrate deoxyribonucleoside kinase from Drosophila melanogaster
    W Knecht
    Department of Microbiology Building 301, Technical University of Denmark, Lyngby, DK 2800, Denmark
    J Mol Biol 301:827-37. 2000
    ..melanogaster. At the same time these mutant enzymes exhibit properties useful in biotechnological and medical applications...
  14. ncbi request reprint Substrate/inhibitor specificities of human deoxycytidine kinase (dCK) and thymidine kinases (TK1 and TK2)
    B Kierdaszuk
    University of Warsaw, Department of Biophysics, Poland
    Adv Exp Med Biol 431:623-7. 1998
    ..The overall data are relevant to the role of these enzymes in "activation" (by phosporylation) of nucleoside analogues with antiviral and antitumor activities...
  15. ncbi request reprint Diverging substrate specificity of pure human thymidine kinases 1 and 2 against antiviral dideoxynucleosides
    B Munch-Petersen
    Department of Biochemistry I, Karolinska Institute, Stockholm, Sweden
    J Biol Chem 266:9032-8. 1991
    ..The much lower tolerance for modifications of the deoxyribose moiety of TK2 as compared to TK1 is important for the design of new antiviral nucleoside analogues intended for use in cells with different expression of TK1 and TK2...
  16. ncbi request reprint Human thymidine kinase 2: molecular cloning and characterisation of the enzyme activity with antiviral and cytostatic nucleoside substrates
    L Wang
    Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, Biomedical Center, Uppsala
    FEBS Lett 443:170-4. 1999
    ..The results demonstrate a broad substrate specificity and complex kinetics, and suggest a role for TK2 in the activation of chemotherapeutic nucleoside analogues...