L Thim

Summary

Affiliation: Novo Nordisk A/S
Country: Denmark

Publications

  1. ncbi request reprint Characterization of human and rat intestinal trefoil factor produced in yeast
    L Thim
    Novo Nordisk A S, Bagsvaerd, Denmark
    Biochemistry 34:4757-64. 1995
  2. doi request reprint Purification and characterization of a new recombinant factor VIII (N8)
    L Thim
    Biopharmaceutical Research Unit, Novo Nordisk A S, Maaloev, Denmark
    Haemophilia 16:349-59. 2010
  3. ncbi request reprint Structure of mammalian trefoil factors and functional insights
    L Thim
    Protein Engineering, Novo Nordisk A S, 2760 Måløv, Denmark
    Cell Mol Life Sci 62:2956-73. 2005
  4. ncbi request reprint Effect of trefoil factors on the viscoelastic properties of mucus gels
    L Thim
    Novo Nordisk A S, Bagsvaerd, Denmark
    Eur J Clin Invest 32:519-27. 2002
  5. ncbi request reprint Purification and characterisation of a new hypothalamic satiety peptide, cocaine and amphetamine regulated transcript (CART), produced in yeast
    L Thim
    Department of Protein Chemistry, Health Care Discovery, Novo Nordisk A S, Bagsvaerd, Denmark
    FEBS Lett 428:263-8. 1998
  6. ncbi request reprint A structural domain (the lid) found in pancreatic lipases is absent in the guinea pig (phospho)lipase
    A Hjorth
    Novo Nordisk A S, Copenhagen, Denmark
    Biochemistry 32:4702-7. 1993
  7. ncbi request reprint Trefoil peptides: from structure to function
    L Thim
    Department of Protein Chemistry, Novo Nordisk A S, Bagsvaerd, Denmark
    Cell Mol Life Sci 53:888-903. 1997
  8. pmc Tissue-specific processing of cocaine- and amphetamine-regulated transcript peptides in the rat
    L Thim
    Health Care Discovery, Novo Nordisk A S, DK 2880 Bagsvaerd, Denmark
    Proc Natl Acad Sci U S A 96:2722-7. 1999
  9. ncbi request reprint Characterization of recombinant human HBP/CAP37/azurocidin, a pleiotropic mediator of inflammation-enhancing LPS-induced cytokine release from monocytes
    P B Rasmussen
    Health Care Discovery, Novo Nordisk, Bagsvaerd, Denmark
    FEBS Lett 390:109-12. 1996
  10. ncbi request reprint Amino acid sequence and posttranslational modifications of human factor VIIa from plasma and transfected baby hamster kidney cells
    L Thim
    Novo Research Institute, Bagsvaerd, Denmark
    Biochemistry 27:7785-93. 1988

Collaborators

Detail Information

Publications43

  1. ncbi request reprint Characterization of human and rat intestinal trefoil factor produced in yeast
    L Thim
    Novo Nordisk A S, Bagsvaerd, Denmark
    Biochemistry 34:4757-64. 1995
    ..Sequence and mass spectrometry analyses as well as peptide mapping showed that the dimer form of both hITF and rITF is mediated by a disulfide bridge between Cys-57 residues of two monomers...
  2. doi request reprint Purification and characterization of a new recombinant factor VIII (N8)
    L Thim
    Biopharmaceutical Research Unit, Novo Nordisk A S, Maaloev, Denmark
    Haemophilia 16:349-59. 2010
    ..In conclusion, the present data show that N8 is a pure and well-characterized FVIII product with biochemical properties that equal other FVIII products...
  3. ncbi request reprint Structure of mammalian trefoil factors and functional insights
    L Thim
    Protein Engineering, Novo Nordisk A S, 2760 Måløv, Denmark
    Cell Mol Life Sci 62:2956-73. 2005
    ..The biological implications of different molecular forms of trefoil factors and their interaction with mucins will be discussed together with other functional insights...
  4. ncbi request reprint Effect of trefoil factors on the viscoelastic properties of mucus gels
    L Thim
    Novo Nordisk A S, Bagsvaerd, Denmark
    Eur J Clin Invest 32:519-27. 2002
    ..It is believed that TFF peptides interact with mucin to increase viscosity but this has never been confirmed. The aims of the present study were to elucidate the direct effect of trefoil peptides on mucus gel formation...
  5. ncbi request reprint Purification and characterisation of a new hypothalamic satiety peptide, cocaine and amphetamine regulated transcript (CART), produced in yeast
    L Thim
    Department of Protein Chemistry, Health Care Discovery, Novo Nordisk A S, Bagsvaerd, Denmark
    FEBS Lett 428:263-8. 1998
    ..When the four CART peptides were intracerebroventricularly injected in fasted mice (0.1 to 2.0 microg) they all produced a dose dependent inhibition of food intake...
  6. ncbi request reprint A structural domain (the lid) found in pancreatic lipases is absent in the guinea pig (phospho)lipase
    A Hjorth
    Novo Nordisk A S, Copenhagen, Denmark
    Biochemistry 32:4702-7. 1993
    ..We propose that this deletion is directly responsible for the anomalous behavior of this enzyme. Thus GPL challenges the classical distinction between lipases, esterases, and phospholipases...
  7. ncbi request reprint Trefoil peptides: from structure to function
    L Thim
    Department of Protein Chemistry, Novo Nordisk A S, Bagsvaerd, Denmark
    Cell Mol Life Sci 53:888-903. 1997
    ..This review summarises our present knowledge of trefoil peptide structures, their sites of expression, and their protection and repair functions, with a focus on the mechanism by which these peptides exert their biological function...
  8. pmc Tissue-specific processing of cocaine- and amphetamine-regulated transcript peptides in the rat
    L Thim
    Health Care Discovery, Novo Nordisk A S, DK 2880 Bagsvaerd, Denmark
    Proc Natl Acad Sci U S A 96:2722-7. 1999
    ..This tissue-specific processing indicates that CART peptides may have different biological functions in the periphery and in the central nervous system...
  9. ncbi request reprint Characterization of recombinant human HBP/CAP37/azurocidin, a pleiotropic mediator of inflammation-enhancing LPS-induced cytokine release from monocytes
    P B Rasmussen
    Health Care Discovery, Novo Nordisk, Bagsvaerd, Denmark
    FEBS Lett 390:109-12. 1996
    ..Furthermore, we have shown that recombinant HBP stimulates in a dose-dependent manner the lipopolysaccharide (LPS)-induced cytokine release from human monocytes...
  10. ncbi request reprint Amino acid sequence and posttranslational modifications of human factor VIIa from plasma and transfected baby hamster kidney cells
    L Thim
    Novo Research Institute, Bagsvaerd, Denmark
    Biochemistry 27:7785-93. 1988
    ..Besides minor differences in the sialic acid and fucose contents, the overall carbohydrate compositions were nearly identical in recombinant factor VIIa and human plasma factor VIIa.(ABSTRACT TRUNCATED AT 250 WORDS)..
  11. ncbi request reprint CART, a new anorectic peptide
    L Thim
    Novo Nordisk A S, Bagsvaerd, Denmark
    Int J Biochem Cell Biol 30:1281-4. 1998
    ..The putative CART receptor is therefore a potential therapeutic target for an anti-obesity drug...
  12. ncbi request reprint Recombinant activated factor VII (rFVIIa): characterization, manufacturing, and clinical development
    B Jurlander
    IO Biopharm, Novo Nordisk A S, Novo Alle, DK 2880 Bagsvaerd, Lyngby, Denmark
    Semin Thromb Hemost 27:373-84. 2001
    ..This review describes the mechanism of action, characterization, manufacturing, and preclinical and current clinical evidence for the efficacy and safety of rFVIIa...
  13. ncbi request reprint Solution structure of the satiety factor, CART, reveals new functionality of a well-known fold
    S Ludvigsen
    Novo Nordisk A S, Novo Alle 1, DK 2880 Bagsvaerd, Denmark
    Biochemistry 40:9082-8. 2001
    ..CART consists mainly of turns and loops spanned by a compact framework composed by a few small stretches of antiparallel beta-sheet common to cystine knots...
  14. ncbi request reprint Adenosine is an agonist of the growth hormone secretagogue receptor
    S Tullin
    Health Care Discovery, Novo Nordisk A S, Novo Nordisk Park, Måløv, Denmark
    Endocrinology 141:3397-402. 2000
    ..Interestingly, neither adenosine nor R-PIA were able to induce GH release from rat pituitary cells in vitro. The implications of the latter finding is discussed...
  15. doi request reprint Pharmacokinetics and ex vivo whole blood clot formation of a new recombinant FVIII (N8) in haemophilia A dogs
    D M Karpf
    Biopharmaceutical Research Unit, Novo Nordisk A S, Måløv, Denmark
    Haemophilia 17:e963-8. 2011
    ..In conclusion, comparison of N8 and Advate ® in haemophilia A dogs revealed similar safety, similar PK and similar effects in whole blood clot formation assays...
  16. pmc Systemically administered trefoil factors are secreted into the gastric lumen and increase the viscosity of gastric contents
    S Kjellev
    Pharmacology Research 4, Novo Nordisk A S, Maaloev, Denmark
    Br J Pharmacol 149:92-9. 2006
    ..We therefore studied the gastric secretion of systemically administered TFF2 and TFF3, and their influence on the viscosity of the secretions...
  17. ncbi request reprint Isolation and characterization of putative trefoil peptide receptors
    L Thim
    Department of Protein Chemistry, Health Care Discovery, Novo Nordisk A S, DK 2880, Bagsvaerd, Denmark
    Regul Pept 90:61-8. 2000
    ..This protein thus fulfils some of the criteria for being a TFF receptor or a TFF binding protein...
  18. ncbi request reprint Hypothalamic CART is a new anorectic peptide regulated by leptin
    P Kristensen
    Health Care Discovery, Novo Nordisk, Bagsvaerd, Denmark
    Nature 393:72-6. 1998
    ..An antiserum against CART increases feeding in normal rats, indicating that CART may be an endogenous inhibitor of food intake in normal animals...
  19. ncbi request reprint Human plasma and recombinant factor VII. Characterization of O-glycosylations at serine residues 52 and 60 and effects of site-directed mutagenesis of serine 52 to alanine
    S Bjoern
    Bioscience Corporate Research, Novo Nordisk A S, Bagsvaerd, Denmark
    J Biol Chem 266:11051-7. 1991
    ..abstract truncated at 400 words)..
  20. ncbi request reprint Structure-function relationships in naturally occurring mutants of pancreatic lipase
    F Carriere
    Novo Nordisk A S, Bagsvaerd, Denmark
    Protein Eng 7:563-9. 1994
    ..These atypical kinetic properties are discussed in the light of amino acid sequence comparison between RP2 and classical pancreatic lipases, based on the closed and open conformations of the 3-D structure of human pancreatic lipase...
  21. ncbi request reprint Activation of cellular invasion by trefoil peptides and src is mediated by cyclooxygenase- and thromboxane A2 receptor-dependent signaling pathways
    S Rodrigues
    INSERM U482, Signal Transduction and Cellular Functions in Diabetes and Digestive Cancers, , 75571 Paris Cedex 12, France
    FASEB J 15:1517-28. 2001
    ..These findings identify a new intracrine/paracrine loop that can be monitored by TFFs and src in inflammatory diseases and progression of colorectal cancers...
  22. ncbi request reprint Secretion of the trefoil factor TFF3 from the isolated vascularly perfused rat colon
    F Moro
    , , Pavillon Hbis, 69437 Cedex 03, Lyon, France
    Regul Pept 101:35-41. 2001
    ..This association presumably involves a disulfide bond. Additionally, the present results suggest a role for enteric nervous system and resident immune cells in mediation of colonic TFF3 secretion...
  23. ncbi request reprint Immunoassays of human trefoil factors 1 and 2: measured on serum from patients with inflammatory bowel disease
    E M Vestergaard
    Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark
    Scand J Clin Lab Invest 64:146-56. 2004
    ....
  24. ncbi request reprint Injected TFF1 and TFF3 bind to TFF2-immunoreactive cells in the gastrointestinal tract in rats
    S S Poulsen
    Anatomy Department B, The Panum Institute, University of Copenhagen, 3 Blegdamsvej, 2200 N, Copenhagen, Denmark
    Regul Pept 115:91-9. 2003
    ..The mucous neck cells of the stomach predominantly take up TFFs with two trefoil domains, indicating a different receptor-like activity in the stomach compared to the rest of the GI tract...
  25. ncbi request reprint High-resolution solution structure of human intestinal trefoil factor and functional insights from detailed structural comparisons with the other members of the trefoil family of mammalian cell motility factors
    X Lemercinier
    Laboratory of Molecular Structure, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, UK
    Biochemistry 40:9552-9. 2001
    ....
  26. ncbi request reprint Trefoil factor family-peptides promote migration of human bronchial epithelial cells: synergistic effect with epidermal growth factor
    M Oertel
    , , Leipziger Strasse 44, D-39120 Magdeburg, Germany
    Am J Respir Cell Mol Biol 25:418-24. 2001
    ..These results suggest that TFF-peptides act as motogens in the human respiratory epithelium triggering rapid repair of damaged mucosa in the course of airway diseases such as asthma...
  27. ncbi request reprint Pancreatic spasmolytic polypeptide: crystallization, circular dichroism analysis, and preliminary X-ray diffraction studies
    M Gajhede
    Department of Physical Chemistry, H C Orsted Institute, University of Copenhagen, Denmark
    Proteins 13:364-8. 1992
    ..With this limitation, the secondary structure analysis predicts 15% alpha-helix, between 10 and 20% antiparallel beta-strand, 10% parallel beta-strand, 15% turn, and 25 to 40% of other structures...
  28. ncbi request reprint Trefoil factor family-interacting proteins
    W R Otto
    Histopathology Unit, Cancer Research UK, 44, Lincoln s Inn Fields, London, WC2A 3PX, United Kingdom
    Cell Mol Life Sci 62:2939-46. 2005
    ..This review describes the expression and, where known, the functions of such proteins...
  29. ncbi request reprint The amino acid sequence of pancreatic spasmolytic polypeptide
    L Thim
    Biochim Biophys Acta 827:410-8. 1985
    ..The alignment of the sequences representing amino acids 14-49 and 63-98 shows pair-wise identical amino acid residues in 18 out of 36 positions, indicating that these two "domains" have been derived from a common gene...
  30. ncbi request reprint Revised amino acid sequence of pancreatic spasmolytic polypeptide exhibits greater similarity with an inducible pS2 peptide found in a human breast cancer cell line
    K Rose
    Departement de Biochimie Medicale, C M U Geneva, Switzerland
    Biochim Biophys Acta 998:297-300. 1989
    ..The revised sequence exhibits greater similarity with pS2 peptide, a 60 residue polypeptide which is induced by oestrogen in the human breast cancer cell line MCF-7 and found in malignant but not in non-malignant breast tissue...
  31. ncbi request reprint Galanin and galanin extended at the N-terminus with seven and nine amino acids are produced in and secreted from the porcine adrenal medulla in almost equal amounts
    M Bersani
    Department of Medical Physiology C, Panum Institute, University of Copenhagen, Denmark
    Endocrinology 129:2693-8. 1991
    ..The processing of preprogalanin in porcine adrenals thus includes the formation and release of galanin, preprogalanin-(24-61), and preprogalanin-(26-61). The signal peptidase cleaves the preprogalanin at either Gly23 or Gly25...
  32. ncbi request reprint Pancreatic spasmolytic polypeptide: first three-dimensional structure of a member of the mammalian trefoil family of peptides
    M Gajhede
    Department of Chemistry, H C Orsted Institute, University of Copenhagen, Denmark
    Structure 1:253-62. 1993
    ..The trefoil peptides are a rapidly growing family of peptides, mainly found in the gastrointestinal tract. There is circumstantial evidence that they stabilize the mucus layer, and may affect the rate of healing of the mucosal epithelium...
  33. ncbi request reprint Isolation to homogeneity and partial characterization of a histo-blood group A defined Fuc alpha 1----2Gal alpha 1----3-N-acetylgalactosaminyltransferase from human lung tissue
    H Clausen
    Biomembrane Institute, University of Washington, Seattle 98119
    J Biol Chem 265:1139-45. 1990
    ..Amino acid composition and N-terminal amino acid sequence of the intact transferase, as well as of peptides released by endolysyl peptidase digest or cyanogen bromide cleavage, are presented...
  34. ncbi request reprint Murine trefoil factor 3 does not directly modulate LPS-mediated dendritic cell function
    M Loos
    Department for Molecular Biomedical Research, VIB, Ghent, Belgium
    Scand J Immunol 66:35-42. 2007
    ..Our results suggest that mTFF3 does not govern immune responses on the level of DC function...
  35. ncbi request reprint Prosomatostatin 1-64 is a major product of somatostatin gene expression in pancreas and gut
    M Bersani
    Institute of Medical Physiology C, Panum Institute, University of Copenhagen, Denmark
    J Biol Chem 264:10633-6. 1989
    ..The eventual hormonal role of pro-SS 1-64 may now be evaluated...
  36. ncbi request reprint Calcium binding in alpha-amylases: an X-ray diffraction study at 2.1-A resolution of two enzymes from Aspergillus
    E Boel
    Chemistry Department, University of York, Heslington, U K
    Biochemistry 29:6244-9. 1990
    ..1-A resolution. The structure of this homologous (over 80%) enzyme and additional kinetic studies support all the structural conclusions regarding both calcium-binding sites...
  37. ncbi request reprint Quantitation and characterization of peptides from the C-terminal flanking region of rat and bovine preprotachykinins
    G P McGregor
    Clinical Research Group for Gastrointestinal Endocrinology, Max Planck Gesellschaft, University of Gottingen, F R G
    J Neurochem 53:1871-7. 1989
    ..A peptide corresponding to the 37-amino-acid residue C-flanking peptide derived from alpha-preprotachykinin was not detected in the extracts as expected from the known low abundance of alpha-preprotachykinin mRNA in rat brain and gut...
  38. ncbi request reprint Activity of various CART peptides in changing locomotor activity in the rat
    H L Kimmel
    Division of Neuroscience, Yerkes Regional Primate Research Center, Emory University, Atlanta, GA 30329, USA
    Neuropeptides 36:9-12. 2002
    ..In summary, rlCART 55-102 and rlCART 62-102 are behaviorally active in this locomotor assay, while rsCART 10-89 and rlCART 55-59 are not...
  39. ncbi request reprint Naturally occurring products of proglucagon 111-160 in the porcine and human small intestine
    T Buhl
    Institute of Medical Physiology C, Panum Institute, Copenhagen, Denmark
    J Biol Chem 263:8621-4. 1988
    ..By isocratic high pressure liquid chromatography human spacer peptide 2 was indistinguishable from synthetic proglucagon 111-122 amide, suggesting that this is the structure of the naturally occurring human peptide...
  40. ncbi request reprint Primary structure of neuromedin U from the rat
    J M Conlon
    Clinical Research Group for Gastrointestinal Endocrinology of the Max Planck Gesellschaft, University of Gottingen, F R G
    J Neurochem 51:988-91. 1988
    ..In particular, the replacement of the Arg16-Arg17 processing site in the porcine peptide by Gly14-Gly15 in the rat means that a peptide corresponding to neuromedin U-8 was not found in the rat intestine...
  41. ncbi request reprint The isolation and sequencing of human gastric inhibitory peptide (GIP)
    A J Moody
    FEBS Lett 172:142-8. 1984
    ..Human GIP differs from porcine GIP at residues 18 and 34. The sequence of human GIP is thus: (Formula: see text) Amino acid residues 18 and 34 are Arg and Ser, respectively, in porcine GIP...
  42. ncbi request reprint The primary structure of porcine glicentin (proglucagon)
    L Thim
    Regul Pept 2:139-50. 1981
    ..Glicentin thus fulfils the structural requirements for being proglucagon...
  43. ncbi request reprint Peptide leucine arginine, a potent immunomodulatory peptide isolated and structurally characterized from the skin of the Northern Leopard frog, Rana pipiens
    A L Salmon
    Departments of Medicine, The Queen's University of Belfast, Belfast BT12 6BJ, United Kingdom
    J Biol Chem 276:10145-52. 2001
    ..e. mature neutrophils. pLR therefore displays biological activity with both granulopoietic progenitor cells and mast cells and thus represents a novel bioactive peptide from frog skin...