Research Topics
| Henrik NielsenSummaryCountry: Denmark Publications
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Publications
The transcriptional landscapeHenrik Nielsen
Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen, Denmark
Methods Mol Biol 703:3-14. 2011..In most of these cases, it is unclear to what extent a function is related to transcription per se or to the RNA products...
Working with RNAHenrik Nielsen
Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen, Denmark
Methods Mol Biol 703:15-28. 2011..The methods are applicable to all types of RNA and require a minimum of specialized equipment...
Group I intron ribozymesHenrik Nielsen
Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen, Denmark
Methods Mol Biol 848:73-89. 2012..Thus, group I intron ribozymes and associated elements found within group I introns is a rich source of biological phenomena. This chapter provides a strategy and protocols for initial characterization of new group I intron ribozymes...
Group I introns: Moving in new directionsHenrik Nielsen
Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen, Denmark Denmark
RNA Biol 6:375-83. 2009..Finally, group I introns can develop into new ribozymes with new biological functions...
A conformational switch in the DiGIR1 ribozyme involved in release and folding of the downstream I-DirI mRNAHenrik Nielsen
Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, DK 2200N Copenhagen, Denmark
RNA 15:958-67. 2009..The role of HEG P1 in GIR1 branching is reminiscent of that of hairpin P-1 in splicing of the Tetrahymena rRNA group I intron and illustrates a general principle in RNA-directed RNA processing...
Outsourcing of experimental workHenrik Nielsen
Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen, Denmark
Methods Mol Biol 703:319-25. 2011..This chapter introduces some of the problems related to analysis of RNA samples in the "omics" context and gives a few hints and key references related to sample preparation for the non-specialist...
The ability to form full-length intron RNA circles is a general property of nuclear group I intronsHenrik Nielsen
Department of Medical Biochemistry and Genetics, The Panum Institute, University of Copenhagen, DK-2200 Copenhagen, Denmark
RNA 9:1464-75. 2003..The biological function of the full-length circles is not known, but the fact that the circles contain the entire genetic information of the intron suggests a role in intron mobility...
A new RNA branching activity: the GIR1 ribozymeHenrik Nielsen
Department of Medical Biochemistry and Genetics, The Panum Institute, University of Copenhagen, Denmark
Blood Cells Mol Dis 38:102-9. 2007..This new example together with work on artificial branching ribozymes and deoxyribozymes shows that branching is facile and points to the possibility that branching reactions could be more prevalent than previously recognized...
An mRNA is capped by a 2', 5' lariat catalyzed by a group I-like ribozymeHenrik Nielsen
Department of Medical Biochemistry and Genetics, Panum Institute, University of Copenhagen, DK 2200N Copenhagen, Denmark
Science 309:1584-7. 2005..The resulting short lariat, by forming a protective 5' cap, might have been useful in a primitive RNA world...
The group I-like ribozyme DiGIR1 mediates alternative processing of pre-rRNA transcripts in Didymium iridisAnna Vader
Department of Medical Biochemistry and Genetics, The Panum Institute, Copenhagen, Denmark
Eur J Biochem 269:5804-12. 2002..This contrasts with the processing order leading to mature rRNA and I-DirI mRNA in growing cells, suggesting an interplay between the two ribozymes of a twin-ribozyme intron...
Blood cell gene expression profiling in rheumatoid arthritis. Discriminative genes and effect of rheumatoid factorLone Frier Bovin
Institute for Inflammation Research IIR 7521, Rigshospitalet National University Hospital, Blegdamsvej 9, DK-2100, Copenhagen, Denmark
Immunol Lett 93:217-26. 2004..Most of the genes discriminative between RA patients and healthy individuals are known to be involved in immunoinflammatory responses, especially those related to altered phagocytic functions...
Sequence and expression analysis of gaps in human chromosome 20Sheroy Minocherhomji
Wilhelm Johannsen Centre for Functional Genome Research, University of Copenhagen, Blegdamsvej 3b, DK 2200 Copenhagen N, Denmark
Nucleic Acids Res 40:6660-72. 2012..Four ncRNAs expressed within the gap at DLGAP4 show elevated expression in the human brain. Our data suggest that unfinished human genome gaps are likely to comprise numerous functional elements...
Northern blotting analysisKnud Josefsen
The Bartholin Institute, Copenhagen University Hospital, Copenhagen, Denmark
Methods Mol Biol 703:87-105. 2011..Recent developments of hybridization membranes and buffers have resulted in increased sensitivity closing the gap to the more laborious nuclease protection experiments...
