Henrik Nielsen

Summary

Country: Denmark

Publications

  1. doi request reprint The transcriptional landscape
    Henrik Nielsen
    Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen, Denmark
    Methods Mol Biol 703:3-14. 2011
  2. doi request reprint Working with RNA
    Henrik Nielsen
    Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen, Denmark
    Methods Mol Biol 703:15-28. 2011
  3. doi request reprint Outsourcing of experimental work
    Henrik Nielsen
    Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen, Denmark
    Methods Mol Biol 703:319-25. 2011
  4. doi request reprint Group I intron ribozymes
    Henrik Nielsen
    Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen, Denmark
    Methods Mol Biol 848:73-89. 2012
  5. ncbi request reprint Group I introns: Moving in new directions
    Henrik Nielsen
    Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen, Denmark Denmark
    RNA Biol 6:375-83. 2009
  6. pmc A conformational switch in the DiGIR1 ribozyme involved in release and folding of the downstream I-DirI mRNA
    Henrik Nielsen
    Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, DK 2200N Copenhagen, Denmark
    RNA 15:958-67. 2009
  7. pmc The ability to form full-length intron RNA circles is a general property of nuclear group I introns
    Henrik Nielsen
    Department of Medical Biochemistry and Genetics, The Panum Institute, University of Copenhagen, DK 2200 Copenhagen, Denmark
    RNA 9:1464-75. 2003
  8. ncbi request reprint A new RNA branching activity: the GIR1 ribozyme
    Henrik Nielsen
    Department of Medical Biochemistry and Genetics, The Panum Institute, University of Copenhagen, Denmark
    Blood Cells Mol Dis 38:102-9. 2007
  9. ncbi request reprint An mRNA is capped by a 2', 5' lariat catalyzed by a group I-like ribozyme
    Henrik Nielsen
    Department of Medical Biochemistry and Genetics, Panum Institute, University of Copenhagen, DK 2200N Copenhagen, Denmark
    Science 309:1584-7. 2005
  10. ncbi request reprint The group I-like ribozyme DiGIR1 mediates alternative processing of pre-rRNA transcripts in Didymium iridis
    Anna Vader
    Department of Medical Biochemistry and Genetics, The Panum Institute, Copenhagen, Denmark
    Eur J Biochem 269:5804-12. 2002

Collaborators

Detail Information

Publications13

  1. doi request reprint The transcriptional landscape
    Henrik Nielsen
    Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen, Denmark
    Methods Mol Biol 703:3-14. 2011
    ..In most of these cases, it is unclear to what extent a function is related to transcription per se or to the RNA products...
  2. doi request reprint Working with RNA
    Henrik Nielsen
    Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen, Denmark
    Methods Mol Biol 703:15-28. 2011
    ..The methods are applicable to all types of RNA and require a minimum of specialized equipment...
  3. doi request reprint Outsourcing of experimental work
    Henrik Nielsen
    Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen, Denmark
    Methods Mol Biol 703:319-25. 2011
    ..This chapter introduces some of the problems related to analysis of RNA samples in the "omics" context and gives a few hints and key references related to sample preparation for the non-specialist...
  4. doi request reprint Group I intron ribozymes
    Henrik Nielsen
    Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen, Denmark
    Methods Mol Biol 848:73-89. 2012
    ..Thus, group I intron ribozymes and associated elements found within group I introns is a rich source of biological phenomena. This chapter provides a strategy and protocols for initial characterization of new group I intron ribozymes...
  5. ncbi request reprint Group I introns: Moving in new directions
    Henrik Nielsen
    Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen, Denmark Denmark
    RNA Biol 6:375-83. 2009
    ..Finally, group I introns can develop into new ribozymes with new biological functions...
  6. pmc A conformational switch in the DiGIR1 ribozyme involved in release and folding of the downstream I-DirI mRNA
    Henrik Nielsen
    Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, DK 2200N Copenhagen, Denmark
    RNA 15:958-67. 2009
    ..The role of HEG P1 in GIR1 branching is reminiscent of that of hairpin P-1 in splicing of the Tetrahymena rRNA group I intron and illustrates a general principle in RNA-directed RNA processing...
  7. pmc The ability to form full-length intron RNA circles is a general property of nuclear group I introns
    Henrik Nielsen
    Department of Medical Biochemistry and Genetics, The Panum Institute, University of Copenhagen, DK 2200 Copenhagen, Denmark
    RNA 9:1464-75. 2003
    ..The biological function of the full-length circles is not known, but the fact that the circles contain the entire genetic information of the intron suggests a role in intron mobility...
  8. ncbi request reprint A new RNA branching activity: the GIR1 ribozyme
    Henrik Nielsen
    Department of Medical Biochemistry and Genetics, The Panum Institute, University of Copenhagen, Denmark
    Blood Cells Mol Dis 38:102-9. 2007
    ..This new example together with work on artificial branching ribozymes and deoxyribozymes shows that branching is facile and points to the possibility that branching reactions could be more prevalent than previously recognized...
  9. ncbi request reprint An mRNA is capped by a 2', 5' lariat catalyzed by a group I-like ribozyme
    Henrik Nielsen
    Department of Medical Biochemistry and Genetics, Panum Institute, University of Copenhagen, DK 2200N Copenhagen, Denmark
    Science 309:1584-7. 2005
    ..The resulting short lariat, by forming a protective 5' cap, might have been useful in a primitive RNA world...
  10. ncbi request reprint The group I-like ribozyme DiGIR1 mediates alternative processing of pre-rRNA transcripts in Didymium iridis
    Anna Vader
    Department of Medical Biochemistry and Genetics, The Panum Institute, Copenhagen, Denmark
    Eur J Biochem 269:5804-12. 2002
    ..This contrasts with the processing order leading to mature rRNA and I-DirI mRNA in growing cells, suggesting an interplay between the two ribozymes of a twin-ribozyme intron...
  11. ncbi request reprint Blood cell gene expression profiling in rheumatoid arthritis. Discriminative genes and effect of rheumatoid factor
    Lone Frier Bovin
    Institute for Inflammation Research IIR 7521, Rigshospitalet National University Hospital, Blegdamsvej 9, DK 2100, Copenhagen, Denmark
    Immunol Lett 93:217-26. 2004
    ..Most of the genes discriminative between RA patients and healthy individuals are known to be involved in immunoinflammatory responses, especially those related to altered phagocytic functions...
  12. pmc Sequence and expression analysis of gaps in human chromosome 20
    Sheroy Minocherhomji
    Wilhelm Johannsen Centre for Functional Genome Research, University of Copenhagen, Blegdamsvej 3b, DK 2200 Copenhagen N, Denmark
    Nucleic Acids Res 40:6660-72. 2012
    ..Four ncRNAs expressed within the gap at DLGAP4 show elevated expression in the human brain. Our data suggest that unfinished human genome gaps are likely to comprise numerous functional elements...
  13. doi request reprint Northern blotting analysis
    Knud Josefsen
    The Bartholin Institute, Copenhagen University Hospital, Copenhagen, Denmark
    Methods Mol Biol 703:87-105. 2011
    ..Recent developments of hybridization membranes and buffers have resulted in increased sensitivity closing the gap to the more laborious nuclease protection experiments...