Moustapha Kassem

Summary

Country: Denmark

Publications

  1. ncbi request reprint Mesenchymal stem cells: biological characteristics and potential clinical applications
    Moustapha Kassem
    Department of Endocrinology and Metabolism, University Hospital of Odense, DK 5000 Odense C, Denmark
    Cloning Stem Cells 6:369-74. 2004
  2. doi request reprint Osteoblastic cells: differentiation and trans-differentiation
    Moustapha Kassem
    Molecular Endocrinology Laboratory KMEB, Department of Endocrinology, Odense University Hospital and Medical Biotechnology Centre, University of Southern Denmark
    Arch Biochem Biophys 473:183-7. 2008
  3. doi request reprint Assessment of bone formation capacity using in vivo transplantation assays: procedure and tissue analysis
    Basem M Abdallah
    Department of Endocrinology, KMEB Laboratory, University Hospital of Odense, Denmark
    Methods Mol Biol 455:89-100. 2008
  4. doi request reprint TiO(2)-based phosphoproteomic analysis of the plasma membrane and the effects of phosphatase inhibitor treatment
    Tine E Thingholm
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark
    J Proteome Res 7:3304-13. 2008
  5. doi request reprint Human serum is as efficient as fetal bovine serum in supporting proliferation and differentiation of human multipotent stromal (mesenchymal) stem cells in vitro and in vivo
    Abdullah Aldahmash
    Endocrine Research Laboratory KMEB, Department of Endocrinology and Metabolism, Odense University Hospital and University of Southern Denmark, Odense, Denmark
    Stem Cell Rev 7:860-8. 2011
  6. doi request reprint Telomerase-deficient mice exhibit bone loss owing to defects in osteoblasts and increased osteoclastogenesis by inflammatory microenvironment
    Hamid Saeed
    Endocrine Research Laboratory, KMEB, Department of Endocrinology and Metabolism, Odense University Hospital and University of Southern Denmark, Odense, Denmark
    J Bone Miner Res 26:1494-505. 2011
  7. doi request reprint Mouse embryonic fibroblasts (MEF) exhibit a similar but not identical phenotype to bone marrow stromal stem cells (BMSC)
    Hamid Saeed
    Endocrine Research Laboratory, KMEB, Department of Endocrinology and Metabolism, Odense University Hospital and University of Southern Denmark, Odense, Denmark
    Stem Cell Rev 8:318-28. 2012
  8. doi request reprint The crosstalk between transforming growth factor-β1 and delta like-1 mediates early chondrogenesis during embryonic endochondral ossification
    Hanna Taipaleenmaki
    Endocrine Research Laboratory KMEB, Department of Endocrinology and Metabolism, Odense University Hospital, Odense, Denmark
    Stem Cells 30:304-13. 2012
  9. doi request reprint Enhanced differentiation of human embryonic stem cells to mesenchymal progenitors by inhibition of TGF-beta/activin/nodal signaling using SB-431542
    Amer Mahmood
    Medical Biotechnology Centre, Department of Endocrinology, University Hospital of Odense, University of South Denmark, Odense, Denmark
    J Bone Miner Res 25:1216-33. 2010
  10. doi request reprint Parameters in three-dimensional osteospheroids of telomerized human mesenchymal (stromal) stem cells grown on osteoconductive scaffolds that predict in vivo bone-forming potential
    Jorge S Burns
    Laboratory for Molecular Endocrinology KMEB, Department of Endocrinology and Metabolism, Odense University Hospital and Medical Biotechnology Center, Odense, Denmark
    Tissue Eng Part A 16:2331-42. 2010

Collaborators

Detail Information

Publications58

  1. ncbi request reprint Mesenchymal stem cells: biological characteristics and potential clinical applications
    Moustapha Kassem
    Department of Endocrinology and Metabolism, University Hospital of Odense, DK 5000 Odense C, Denmark
    Cloning Stem Cells 6:369-74. 2004
    ....
  2. doi request reprint Osteoblastic cells: differentiation and trans-differentiation
    Moustapha Kassem
    Molecular Endocrinology Laboratory KMEB, Department of Endocrinology, Odense University Hospital and Medical Biotechnology Centre, University of Southern Denmark
    Arch Biochem Biophys 473:183-7. 2008
    ....
  3. doi request reprint Assessment of bone formation capacity using in vivo transplantation assays: procedure and tissue analysis
    Basem M Abdallah
    Department of Endocrinology, KMEB Laboratory, University Hospital of Odense, Denmark
    Methods Mol Biol 455:89-100. 2008
    ..This chapter describes the use of in vivo transplantation methods in testing bone formationpotential of human mesenchymal stem cells...
  4. doi request reprint TiO(2)-based phosphoproteomic analysis of the plasma membrane and the effects of phosphatase inhibitor treatment
    Tine E Thingholm
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark
    J Proteome Res 7:3304-13. 2008
    ..In summary, our analytical strategy enables functional phosphoproteomic analysis of stem cell differentiation and cell surface biomarker discovery using very low amounts of starting material...
  5. doi request reprint Human serum is as efficient as fetal bovine serum in supporting proliferation and differentiation of human multipotent stromal (mesenchymal) stem cells in vitro and in vivo
    Abdullah Aldahmash
    Endocrine Research Laboratory KMEB, Department of Endocrinology and Metabolism, Odense University Hospital and University of Southern Denmark, Odense, Denmark
    Stem Cell Rev 7:860-8. 2011
    ..However, routine use of fetal bovine sera (FBS) for their in vitro expansion is not optimal and may pose a health risk for patients...
  6. doi request reprint Telomerase-deficient mice exhibit bone loss owing to defects in osteoblasts and increased osteoclastogenesis by inflammatory microenvironment
    Hamid Saeed
    Endocrine Research Laboratory, KMEB, Department of Endocrinology and Metabolism, Odense University Hospital and University of Southern Denmark, Odense, Denmark
    J Bone Miner Res 26:1494-505. 2011
    ..Thus telomerization of MSCs may provide a novel approach for abolishing age-related bone loss...
  7. doi request reprint Mouse embryonic fibroblasts (MEF) exhibit a similar but not identical phenotype to bone marrow stromal stem cells (BMSC)
    Hamid Saeed
    Endocrine Research Laboratory, KMEB, Department of Endocrinology and Metabolism, Odense University Hospital and University of Southern Denmark, Odense, Denmark
    Stem Cell Rev 8:318-28. 2012
    ..Due to their enhanced cell growth, they may represent a good alternative for BMSC in studying molecular mechanisms of stem cell commitment and differentiation to osteoblasts, adipocytes and chondrocytes...
  8. doi request reprint The crosstalk between transforming growth factor-β1 and delta like-1 mediates early chondrogenesis during embryonic endochondral ossification
    Hanna Taipaleenmaki
    Endocrine Research Laboratory KMEB, Department of Endocrinology and Metabolism, Odense University Hospital, Odense, Denmark
    Stem Cells 30:304-13. 2012
    ..The crosstalk between TGF-β1 and Dlk1/FA1 was shown to promote early chondrogenesis during the embryonic endochondral ossification process...
  9. doi request reprint Enhanced differentiation of human embryonic stem cells to mesenchymal progenitors by inhibition of TGF-beta/activin/nodal signaling using SB-431542
    Amer Mahmood
    Medical Biotechnology Centre, Department of Endocrinology, University Hospital of Odense, University of South Denmark, Odense, Denmark
    J Bone Miner Res 25:1216-33. 2010
    ..In conclusion, our data provides a simple and versatile protocol for directing the differentiation of hESCs into a myogenic lineage and then further into mesenchymal progenitors by blocking the TGF-beta signaling pathway...
  10. doi request reprint Parameters in three-dimensional osteospheroids of telomerized human mesenchymal (stromal) stem cells grown on osteoconductive scaffolds that predict in vivo bone-forming potential
    Jorge S Burns
    Laboratory for Molecular Endocrinology KMEB, Department of Endocrinology and Metabolism, Odense University Hospital and Medical Biotechnology Center, Odense, Denmark
    Tissue Eng Part A 16:2331-42. 2010
    ....
  11. doi request reprint Selective isolation and differentiation of a stromal population of human embryonic stem cells with osteogenic potential
    Linda Harkness
    Department of Endocrinology and Metabolism, Laboratory for Molecular Endocrinology KMEB, Medical Biotechnology Centre MBC, Winsløwparken 25, University of Southern Denmark, 5000 Odense C, Denmark
    Bone 48:231-41. 2011
    ..In conclusion, hESC-stromal can be isolated from hESC cultures and represent a good source for obtaining cells with osteogenic differentiation potential suitable for regenerative medicine protocols...
  12. doi request reprint Levels of serotonin, sclerostin, bone turnover markers as well as bone density and microarchitecture in patients with high-bone-mass phenotype due to a mutation in Lrp5
    Morten Frost
    Department of Endocrinology and Metabolism, Odense University Hospital, Odense, Denmark
    J Bone Miner Res 26:1721-8. 2011
    ..The phenotype appeared to progress with age, but BTM did not suggest increased bone formation...
  13. pmc MicroRNA-138 regulates osteogenic differentiation of human stromal (mesenchymal) stem cells in vivo
    Tilde Eskildsen
    Molecular Endocrinology Laboratory, KMEB, Odense University Hospital, University of South Denmark, DK 5000 Odense C, Denmark
    Proc Natl Acad Sci U S A 108:6139-44. 2011
    ..Our findings suggest that pharmacological inhibition of miR-138 by antimiR-138 could represent a therapeutic strategy for enhancing bone formation in vivo...
  14. doi request reprint Isolation and differentiation of chondrocytic cells derived from human embryonic stem cells using dlk1/FA1 as a novel surface marker
    Linda Harkness
    Molecular Endocrinology Laboratory KMEB, Medical Biotechnology Centre, Odense University Hospital, University of South Denmark, DK 5000 Odense C, Denmark
    Stem Cell Rev 5:353-68. 2009
    ..Tracking dlk1/FA1 expression as a mesoderm/chondroprogenitor surface marker provides a novel strategy for designing clinically relevant protocols to direct the differentiation of hESC into chondrocytes...
  15. doi request reprint Activation of non-canonical Wnt/JNK pathway by Wnt3a is associated with differentiation fate determination of human bone marrow stromal (mesenchymal) stem cells
    Weimin Qiu
    Laboratory for Molecular Endocrinology KMEB, Department of Endocrinology and Metabolism, University Hospital of Odense, Odense C, Denmark
    Biochem Biophys Res Commun 413:98-104. 2011
    ..In conclusion, canonical and non-canonical Wnt signaling cooperate in determining MSC differentiation fate...
  16. pmc Tumor necrosis factor receptor superfamily member 19 (TNFRSF19) regulates differentiation fate of human mesenchymal (stromal) stem cells through canonical Wnt signaling and C/EBP
    Weimin Qiu
    Laboratory for Molecular Endocrinology KMEB, Department of Endocrinology and Metabolism, University Hospital of Odense, J B Winsløws Vej 25, 1, DK 5000 Odense C, Denmark
    J Biol Chem 285:14438-49. 2010
    ..In conclusion, we revealed a novel function of TNFRSF19 as a factor mediating differentiation signals that determine the hMSC differentiating fate into osteoblasts or adipocytes...
  17. doi request reprint Wnt signalling mediates the cross-talk between bone marrow derived pre-adipocytic and pre-osteoblastic cell populations
    Hanna Taipaleenmaki
    Department of Endocrinology and Metabolism, Endocrine Research Laboratory KMEB, Odense University Hospital, Odense, Denmark
    Exp Cell Res 317:745-56. 2011
    ..In conclusion, cross-talk exists between different populations of MSC in the bone marrow, and Wnt signalling functions as a molecular switch that determines the balance between osteoblastogenesis and adipogenesis...
  18. doi request reprint Senescence-associated intrinsic mechanisms of osteoblast dysfunctions
    Moustapha Kassem
    Department of Endocrinology and Metabolism, University Hospital of Odense, Odense, Denmark
    Aging Cell 10:191-7. 2011
    ..The aim of this review is to provide a summary of the intrinsic senescence mechanisms affecting osteoblastic functions and how they can be targeted to abolish age-related osteoblastic dysfunction and bone loss associated with aging...
  19. doi request reprint Mechanisms in endocrinology: micro-RNAs: targets for enhancing osteoblast differentiation and bone formation
    Hanna Taipaleenmaki
    Molecular Endocrinology Laboratory, KMEB, Department of Endocrinology and Metabolism, Medical Biotechnology Center, Odense University Hospital, University of Southern Denmark, SDU, DK 5000 Odense C, Denmark
    Eur J Endocrinol 166:359-71. 2012
    ..This review highlights the current knowledge of miRNA biology and their role in bone formation and discusses their potential use in future therapeutic applications for metabolic bone diseases...
  20. ncbi request reprint Patients with high bone mass phenotype exhibit enhanced osteoblast differentiation and inhibition of adipogenesis of human mesenchymal stem cells
    Weimin Qiu
    Laboratory for Molecular Endocrinology KMEB, Department of Endocrinology and Metabolism, University Hospital of Odense, Odense C, Denmark
    J Bone Miner Res 22:1720-31. 2007
    ..Our in vivo and in vitro results show that activated mutation T253I of LRP5 enhances osteogenesis and inhibits adipogenesis. Inactivating mutation T244M of LRP5 exerts opposite effects...
  21. doi request reprint New factors controlling the balance between osteoblastogenesis and adipogenesis
    Basem M Abdallah
    Endocrine Research Laboratory KMEB, Department of Endocrinology and Metabolism, Odense University Hospital, Odense, Denmark
    Bone 50:540-5. 2012
    ....
  22. ncbi request reprint Human bone-marrow-derived mesenchymal stem cells: biological characteristics and potential role in therapy of degenerative diseases
    Moustapha Kassem
    Department of Endocrinology and Metabolism, University Hospital of Odense, 5000, Odense C, Denmark
    Cell Tissue Res 331:157-63. 2008
    ..We also review the current status of their clinical use. Areas in which research is needed to enhance the clinical use of MSC are emphasized...
  23. doi request reprint Distinct GAGE and MAGE-A expression during early human development indicate specific roles in lineage differentiation
    Morten F Gjerstorff
    Medical Biotechnology Center, University of Southern Denmark, Winsloewparken 25, DK 5000 Odense C, Denmark
    Hum Reprod 23:2194-201. 2008
    ..We have evaluated the potential role of CTAs in early human development...
  24. doi request reprint Patients with high-bone-mass phenotype owing to Lrp5-T253I mutation have low plasma levels of serotonin
    Morten Frost
    Department of Endocrinology and Metabolism, Laboratory for Molecular Endocrinology KMEB, Medical Biotechnology Centre, University of Southern Denmark, Odense, Denmark
    J Bone Miner Res 25:673-5. 2010
    ..16 +/- 0.28 ng/mL versus 3.51 +/- 0.49 ng/mL, respectively, p < .05). Our data support the hypothesis that circulating serotonin levels mediate the increased bone mass resulting from gain-of-function mutations in Lrp5 in humans...
  25. doi request reprint Teratoma formation by human embryonic stem cells is site dependent and enhanced by the presence of Matrigel
    Tatyana A Prokhorova
    Molecular Endocrinology Laboratory KMEB, Department of Endocrinology and Metabolism, University Hospital of Odense and Medical Biotechnology Center, University of Southern Denmark, Winsløwparken, Odense, Denmark
    Stem Cells Dev 18:47-54. 2009
    ..Also, teratoma formation can be employed to study the development defects exhibited by the chromosomally abnormal hESC lines...
  26. pmc Estrogen inhibits Dlk1/FA1 production: a potential mechanism for estrogen effects on bone turnover
    Basem M Abdallah
    Endocrine Research Laboratory KMEB, Department of Endocrinology and Metabolism, Odense University Hospital and University of Southern Denmark, Odense, Denmark
    J Bone Miner Res 26:2548-51. 2011
    ..001). In conclusion, s-Dlk1/FA1 is influenced by E-deficiency and is correlated with bone turnover. Increased levels of s-Dlk1/FA1 in postmenopausal women may be a mechanism mediating the effects of estrogen deficiency on bone turnover...
  27. pmc Decellularized matrix from tumorigenic human mesenchymal stem cells promotes neovascularization with galectin-1 dependent endothelial interaction
    Jorge S Burns
    Molecular Endocrinology Laboratory KMEB, Department of Endocrinology and Metabolism, Odense University Hospital, University of Southern Denmark, Odense, Denmark
    PLoS ONE 6:e21888. 2011
    ..By exploring the heterogeneity in hMSC-TERT20 clones we aimed to understand molecular mechanisms by which mesenchymal stem cells may promote neovascularization...
  28. ncbi request reprint Stem cells: potential therapy for age-related diseases
    Moustapha Kassem
    Department of Endocrinology and Metabolism, University Hospital of Odense, Denmark
    Ann N Y Acad Sci 1067:436-42. 2006
    ..While it is expected that cellular medicines will decrease the burden of several age-related diseases, it is not clear whether they can change the course of the aging process itself and thus prolong human life...
  29. pmc The use of hTERT-immortalized cells in tissue engineering
    Moustapha Kassem
    Department of Endocrinology and Metabolism, University Hospital of Odense, DK 5000, Odense C, Denmark
    Cytotechnology 45:39-46. 2004
    ..Thus, safety issues should be studied carefully before using the telomerized tissues in the clinic. Alternatively, the development of conditional or intermittent telomerase activation protocols is needed...
  30. ncbi request reprint Activin B mediated induction of Pdx1 in human embryonic stem cell derived embryoid bodies
    Ulrik Frandsen
    KMEB Laboratory, Medical Biotechnology Center, Winslevparken 25 1, DK 5000 Odense C, Denmark
    Biochem Biophys Res Commun 362:568-74. 2007
    ..The data suggest that additional suppression of Shh signaling may be required to allow for proper specification of pancreatic cell lineages in hESCs...
  31. ncbi request reprint Dlk1/FA1 is a novel endocrine regulator of bone and fat mass and its serum level is modulated by growth hormone
    Basem M Abdallah
    Department of Endocrinology, Clinic for Molecular Endocrinology Treatment Laboratory, Odense University Hospital, Medical Biotechnology Center, University of South Denmark, DK 5000 Odense C, Denmark
    Endocrinology 148:3111-21. 2007
    ..FA1 thus provides a novel class of developmental molecules that regulate physiological functions of the postnatal organisms...
  32. ncbi request reprint Inhibition of osteoblast differentiation but not adipocyte differentiation of mesenchymal stem cells by sera obtained from aged females
    Basem M Abdallah
    Department of Endocrinology and Metabolism, University Hospital of Odense, DK 5000 Odense C, Denmark
    Bone 39:181-8. 2006
    ..In conclusion, age-related changes in the serum composition and possibly hMSC microenvironment may contribute to the impaired osteoblast functions with aging. The factors mediating these changes remain to be determined...
  33. ncbi request reprint Telomerase expression extends the proliferative life-span and maintains the osteogenic potential of human bone marrow stromal cells
    Janne L Simonsen
    Department of Endocrinology and Metabolism, University Hospital of Aarhus, DK 8000 Aarhus C, Denmark
    Nat Biotechnol 20:592-6. 2002
    ..These results suggest that ectopic expression of telomerase in hMSCs prevents senescence-associated impairment of osteoblast functions...
  34. ncbi request reprint Tumorigenic heterogeneity in cancer stem cells evolved from long-term cultures of telomerase-immortalized human mesenchymal stem cells
    Jorge S Burns
    Department of Endocrinology and Metabolism and Institute of Pathology, Odense University Hospital, Medical Biotechnology Center, Winsløwparken 25, DK 5000 Odense C, Denmark
    Cancer Res 65:3126-35. 2005
    ..Thus, hMSC-TERT20 clones represent cancer stem cells with hierarchical tumorigenicity, providing new models to explore the stem cell hypothesis for cancer...
  35. ncbi request reprint Mesenchymal stem cells: cell biology and potential use in therapy
    Moustapha Kassem
    Department of Endocrinology and Metabolism, University Hospital of Odense, DK 5000 Odense C, Denmark
    Basic Clin Pharmacol Toxicol 95:209-14. 2004
    ..The results of these initial trials are very encouraging and several clinical trials are under way to study the efficacy and long-term safety of therapeutics based on mesenchymal stem cells...
  36. ncbi request reprint Adult human mesenchymal stem cell as a target for neoplastic transformation
    Nedime Serakinci
    Department of Endocrinology, University Hospital of Odense, Denmark
    Oncogene 23:5095-8. 2004
    ..These data suggest that the adult hMSCs can be targets for neoplastic transformation and have implications for the development of novel anticancer therapeutics and for the use of hMSC in tissue engineering and transplantation protocols...
  37. ncbi request reprint Maintenance of differentiation potential of human bone marrow mesenchymal stem cells immortalized by human telomerase reverse transcriptase gene despite [corrected] extensive proliferation
    Basem M Abdallah
    Laboratory for Molecular Endocrinology, Department of Endocrinology and Metabolism, University Hospital of Odense, Odense, Denmark
    Biochem Biophys Res Commun 326:527-38. 2005
    ....
  38. ncbi request reprint Multilineage differentiation of porcine bone marrow stromal cells associated with specific gene expression pattern
    Lijin Zou
    Orthopaedic Research Laboratory, Aarhus University Hospital, 8000 Aarhus C, Denmark
    J Orthop Res 26:56-64. 2008
    ....
  39. ncbi request reprint Blood vessels engineered from human cells
    Moustapha Kassem
    Lancet 366:891-2; author reply 892-3. 2005
  40. ncbi request reprint Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells
    Karin Stenderup
    University Department of Endocrinology and Metabolism, University Hospital of Aarhus, DK 8000 Aarhus C, Denmark
    Bone 33:919-26. 2003
    ..In conclusion, aging is associated with decreased proliferative capacity of osteoprogenitor cells, suggesting that decreased osteoblastic cell number, and not function, leads to age-related decrease in bone formation...
  41. ncbi request reprint Expression of LRP1 by human osteoblasts: a mechanism for the delivery of lipoproteins and vitamin K1 to bone
    Andreas Niemeier
    Department of Orthopaedics, University Hospital Hamburg Eppendorf, Hamburg, Germany
    J Bone Miner Res 20:283-93. 2005
    ..Here we describe the expression of the multifunctional LRP1 by human osteoblasts in vitro and in vivo. We provide evidence that LRP1 plays an important role in the uptake of postprandial lipoproteins and vitamin K1 by human osteoblasts...
  42. ncbi request reprint Effect of hyaluronan on osteogenic differentiation of porcine bone marrow stromal cells in vitro
    Lijin Zou
    Orthopaedic Research Laboratory, Aarhus University Hospital, 8000 Aarhus C, Denmark
    J Orthop Res 26:713-20. 2008
    ..These results suggest that exogenous HA stimulates endogenous HA, which together may play a synergetic role in osteogenic differentiation under osteoinducing conditions although gene expression was inhibited at the early stage...
  43. ncbi request reprint Induction of adipocyte-like phenotype in human mesenchymal stem cells by hypoxia
    Trine Fink
    Laboratory for Stem Cell Research, Department of Health Science and Technology, Aalborg University, Aarhus, Denmark
    Stem Cells 22:1346-55. 2004
    ..In conclusion, hypoxia appears to exert a potent lipogenic effect independent of PPAR-gamma2 maturation pathway...
  44. ncbi request reprint [Mesenchymal stem cell therapy--the key to regenerative medicine?]
    Moustapha Kassem
    Endokrinologisk Afdeling M, Odense Universitetshospital, DK 5000 Odense C
    Ugeskr Laeger 165:4852-5. 2003
  45. ncbi request reprint Regulation of human skeletal stem cells differentiation by Dlk1/Pref-1
    Basem M Abdallah
    Department of Endocrinology, Univerity Hospital of Odense, Odense, Denmark
    J Bone Miner Res 19:841-52. 2004
    ..Dlk1/Pref-1 is expressed in bone and cultured osteoblasts, and its constitutive overexpression led to inhibition of osteoblast and adipocyte differentiation of human marrow stromal cells...
  46. ncbi request reprint Differential expression profiling of membrane proteins by quantitative proteomics in a human mesenchymal stem cell line undergoing osteoblast differentiation
    Leonard J Foster
    UBC Center for Proteomics, Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, Canada
    Stem Cells 23:1367-77. 2005
    ..In conclusion, MS-based proteomics can reveal novel markers for MSCs that can be used for their isolation and for monitoring OB differentiation...
  47. ncbi request reprint Selenium supplementation restores the antioxidative capacity and prevents cell damage in bone marrow stromal cells in vitro
    Regina Ebert
    Musculosceletal Research Center, Orthopaedic Department, University of Wurzburg, Brettreichstrasse 11, D 97074 Wurzburg, Germany
    Stem Cells 24:1226-35. 2006
    ..Selenite supplementation of BMSC cultures appears to be an important countermeasure to restore their antioxidative capacity and to reduce cell damage in the context of tissue engineering and transplantation procedures...
  48. ncbi request reprint Playing with bone and fat
    Jeffrey M Gimble
    Stem Cell Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, 70808, USA
    J Cell Biochem 98:251-66. 2006
    ..In this review, we focus on the recent literature exploring the mechanisms underlying these differentiation events and discuss their implications relevant to osteoporosis and regenerative medicine...
  49. doi request reprint Flow perfusion culture of human mesenchymal stem cells on silicate-substituted tricalcium phosphate scaffolds
    Lea Bjerre
    Orthopaedic Research Laboratory, Laboratory for Molecular Orthopaedics, Aarhus University Hospital, Norrebrogade 44, Building 1A, 8000 Aarhus C, Denmark
    Biomaterials 29:2616-27. 2008
    ..A viable and homogenous ex vivo bone construct with superior osteogenic properties was produced in dynamic culture and may provide a replacement for autologous grafts...
  50. doi request reprint Human mesenchymal stem cell proliferation is regulated by PGE2 through differential activation of cAMP-dependent protein kinase isoforms
    Charlotte Ramstad Kleiveland
    Institute of Immunology, Rikshospitalet Radiumhospitalet Medical Center, Oslo, Norway
    Exp Cell Res 314:1831-8. 2008
    ..Hence, the two different PKA isoforms seem to have opposing functions in the regulation of proliferation and differentiation in these cells...
  51. ncbi request reprint Morphology, proliferation, and osteogenic differentiation of mesenchymal stem cells cultured on titanium, tantalum, and chromium surfaces
    Maik Stiehler
    Orthopedic Research Laboratory, Clinical Institute, Aarhus University Hospital, Aarhus, Denmark
    J Biomed Mater Res A 86:448-58. 2008
    ..In conclusion, Ta metal, as an alternative for Ti, can be considered as a promising biocompatible material, whereas further studies are needed to fully understand the role of Cr and its alloys in bone implants...
  52. ncbi request reprint Effects of high glucose on mesenchymal stem cell proliferation and differentiation
    Yu Ming Li
    Orthopedic Center for Musculoskeletal Research, Orthopedic Department, University of Wurzburg, Brettreichstrasse 11, D 97074 Wurzburg, Germany
    Biochem Biophys Res Commun 363:209-15. 2007
    ..Effects of HG on the transient amplifying compartment of MSC may differ from those in mature cells. Further research is needed to unravel the molecular mechanisms of HG resistance of MSC...
  53. ncbi request reprint Telomerase promoter reprogramming and interaction with general transcription factors in the human mesenchymal stem cell
    Nedime Serakinci
    University of Southern Denmark, Institute of Medical Biology, Department of Anatomy and Neurobiology, Odense C, Denmark
    Regen Med 1:125-31. 2006
    ..hTR expression is also increased in TSA-treated hMSCs, concomitant with changes in Pol II and TFIIB dynamics...
  54. ncbi request reprint dlk1/FA1 regulates the function of human bone marrow mesenchymal stem cells by modulating gene expression of pro-inflammatory cytokines and immune response-related factors
    Basem M Abdallah
    KMEB Laboratory, Medical Biotechnology Center, Odense University Hospital, Southern Denmark University, DK 5000 Odense, Denmark
    J Biol Chem 282:7339-51. 2007
    ..quot; Our findings also demonstrate a role for Dlk1 in mediating the immune response...
  55. ncbi request reprint Mesenchymal stem cell ingrowth and differentiation on coralline hydroxyapatite scaffolds
    Tina Mygind
    Orthopedic Research Laboratory, Laboratory for Molecular Orthopedics, Clinical Institute, Aarhus University Hospital, Norrebrogade 44 bldg 1A, DK 8000 Aarhus C, Denmark
    Biomaterials 28:1036-47. 2007
    ..Therefore, spinner flask cultivation is an easy-to-use inexpensive system for cultivating hMSCs on small to intermediate size 3D scaffolds...
  56. ncbi request reprint Heat shock-induced enhancement of osteoblastic differentiation of hTERT-immortalized mesenchymal stem cells
    Rune Nørgaard
    Laboratory of Cellular Ageing, Danish Centre for Molecular Gerontology, Department of Molecular Biology, University of Aarhus, Denmark
    Ann N Y Acad Sci 1067:443-7. 2006
    ..Our results indicate that the exposure of cells to mild heat stress enhances the extent of differentiation of hMSCs by 12% to 42%. These effects are an expression of the phenomenon of mild stress-induced hormesis...
  57. ncbi request reprint Effects of bone protein extract on human mesenchymal stem cells proliferation and differentiation
    Charlotte Woo
    Orthopaedic Research Laboratory, Aarhus University Hospital, Nørrebrogade 44, Building 1A, DK 8000 Aarhus C, Denmark
    J Biomed Mater Res A 79:552-6. 2006
    ..Our study demonstrated significant metabolic effects of BPE on hMSC-TERT cells, which were highly dependant on the differentiated state of the cells...
  58. ncbi request reprint Characterization of lipid metabolism in insulin-sensitive adipocytes differentiated from immortalized human mesenchymal stem cells
    Janne Prawitt
    Department of Biochemistry and Molecular Biology II Molecular Cell Biology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Exp Cell Res 314:814-24. 2008
    ..Despite this increased expression, the receptor-mediated endocytosis of lipoproteins was decreased in differentiated adipocytes, suggesting that these proteins may have an additional function in adipose tissue beyond lipoprotein uptake...