Kenneth Alan Howard
Affiliation: Interdisciplinary Nanoscience Center
- Nanocarrier stimuli-activated gene deliveryKenneth A Howard
Interdisciplinary Nanoscience Center and Department of Molecular Biology, University of Aarhus, 8000 Aarhus C, Denmark
Small 3:54-7. 2007
- Polycation-based nanoparticle delivery for improved RNA interference therapeuticsKenneth A Howard
University of Aarhus, Interdisciplinary Nanoscience Center iNANO and Department of Molecular Biology, 8000 Aarhus, Denmark
Expert Opin Biol Ther 7:1811-22. 2007..Issues including use of stimuli-responsive systems for intracellular trafficking of siRNA are discussed as part of necessary future directives towards the development of RNA-based clinical therapeutics...
- Chitosan/siRNA nanoparticle-mediated TNF-alpha knockdown in peritoneal macrophages for anti-inflammatory treatment in a murine arthritis modelKenneth A Howard
Interdisciplinary Nanoscience Center iNANO, University of Aarhus, Aarhus C, Denmark
Mol Ther 17:162-8. 2009..This work demonstrates nanoparticle-mediated TNF-alpha knockdown in peritoneal macrophages as a method to reduce both local and systemic inflammation, thereby presenting a novel strategy for arthritis treatment...
- Delivery of RNA interference therapeutics using polycation-based nanoparticlesKenneth Alan Howard
Interdisciplinary Nanoscience Center iNANO, Department of Molecular Biology, University of Aarhus, 8000 Aarhus C, Denmark
Adv Drug Deliv Rev 61:710-20. 2009..This work highlights the versatile nature of polycation-based nanoparticles to fulfil the delivery requirements for RNA molecules with flexibility in design to evolve alongside an expanding repertoire of RNAi-based drugs...
- Chitosan/siRNA nanoparticles encapsulated in PLGA nanofibers for siRNA deliveryMenglin Chen
Interdisciplinary Nanoscience Center iNANO, Aarhus University, DK 8000 Aarhus C, Denmark
ACS Nano 6:4835-44. 2012..The interesting release profile was further investigated for siRNA transfection, where the encapsulated chitosan/siRNA NPs exhibited up to 50% EGFP gene silencing activity after 48 h post-transfection on H1299 cells...
- RNAi using a chitosan/siRNA nanoparticle system: in vitro and in vivo applicationsMorten Østergaard Andersen
Interdisciplinary Nanoscience Center iNANO, Department of Molecular Biology, University of Aarhus, Aarhus C, Denmark
Methods Mol Biol 555:77-86. 2009..The method exploits the mucoadhesive and mucopermeable properties of this cationic polysaccharide to deliver siRNA across mucosal epithelium and provides a platform for targeting human diseases with RNAi therapeutics...
- The effect of chemical modification and nanoparticle formulation on stability and biodistribution of siRNA in miceShan Gao
Department of Molecular Biology, University of Aarhus, Denmark
Mol Ther 17:1225-33. 2009..This comparative study highlights improvements to siRNA stability and pharmacokinetics, key determinants for development of clinically relevant RNAi therapeutics...
- Polycation-based nanoparticle delivery of RNAi therapeutics: adverse effects and solutionsBorja Ballarín-González
Interdisciplinary Nanoscience Center iNANO, Department of Molecular Biology and Genetics, University of Aarhus, Aarhus, Denmark
Adv Drug Deliv Rev 64:1717-29. 2012..A greater understanding of the cellular mechanisms involved allows design-based solutions for rationale development of safe, effective and clinically relevant polyplex-based RNAi drugs...