Jan Holm

Summary

Country: Denmark

Publications

  1. ncbi Binding of radiolabeled folate and 5-methyltetrahydrofolate to cow's milk folate binding protein at pH 7.4 and 5.0. Relationship to concentration and polymerization equilibrium of the purified protein
    J Holm
    Department of Clinical Chemistry, Central Hospital, Herning, Denmark
    Biosci Rep 21:733-43. 2001
  2. ncbi Ligand binding characteristics and aggregation behavior of purified cow's milk folate binding protein depends on the presence of amphiphatic substances including cholesterol, phospholipids, and synthetic detergents
    Jan Holm
    Department of Clinical Chemistry, Central Hospital Herning, DK 7400, Denmark
    Biosci Rep 22:431-41. 2002
  3. ncbi A combination of cation exchange and ligand-affinity chromatography for purification of two molecular species of the folate binding protein in human milk, one equipped with a hydrophobic glycosyl phosphatidylinositol tail: characterization of hydrophobici
    Jan Holm
    Department of Clinical Chemistry, Central Hospital, Herning, DK 7400, Denmark
    Biosci Rep 22:443-54. 2002
  4. ncbi Ligand binding characteristics of two molecular forms, one equipped with a hydrophobic glycosyl phosphatidylinositol tail, of the folate binding protein purified from human milk
    Jan Holm
    Department of Clinical Chemistry, Central Hospital Herning, DK 7400, Denmark
    Biosci Rep 22:455-63. 2002
  5. ncbi Ligand binding and polymerization characteristics of human milk folate binding protein depend on concentration of purified protein and presence of amphiphatic substances
    Jan Holm
    Department of Clinical Chemistry, Central Hospital Herning, DK 7400, Denmark
    Biosci Rep 23:77-85. 2003
  6. ncbi Characterization of a high affinity folate binding protein in porcine serum: ionic charge, concentration-dependent polymerization and ligand binding mechanism
    Jan Holm
    Department of Clinical Chemistry, Hillerod Hospital, DK 3400, Denmark
    Biosci Rep 23:339-51. 2003
  7. ncbi Megalin binds and mediates cellular internalization of folate binding protein
    Henrik Birn
    Department of Cell Biology, Institute of Anatomy, University of Aarhus, Denmark
    FEBS J 272:4423-30. 2005
  8. ncbi A minor fraction of a high-affinity folate binding protein from the epididymis is associated with membranous vesicles and spermatozoa in human semen
    Johan Malm
    Division of Clinical Chemistry, Department of Laboratory Medicine, Lund University, Malmo University Hospital, 205 02 Malmo, Sweden
    Int J Androl 28:267-74. 2005
  9. ncbi A novel specific heparin-binding activity of bovine folate-binding protein characterized by capillary electrophoresis
    Niels H H Heegaard
    Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark
    Electrophoresis 27:1122-7. 2006
  10. ncbi Application of near-infrared and Fourier transform infrared spectroscopy in the characterization of ligand-induced conformation changes in folate binding protein purified from bovine milk: influence of buffer type and pH
    Susanne W Bruun
    Biochemistry and Nutrition Group, BioCentrum DTU, Technical University of Denmark, Kgs Lyngby, Denmark
    Appl Spectrosc 60:737-46. 2006

Collaborators

Detail Information

Publications11

  1. ncbi Binding of radiolabeled folate and 5-methyltetrahydrofolate to cow's milk folate binding protein at pH 7.4 and 5.0. Relationship to concentration and polymerization equilibrium of the purified protein
    J Holm
    Department of Clinical Chemistry, Central Hospital, Herning, Denmark
    Biosci Rep 21:733-43. 2001
    ..0 surpasses 5-methyltetrahydrofolate both with regard to binding affinity and ability to induce polymerization suggests that ligand binding is associated with conformational changes of the protein which favor polymerization...
  2. ncbi Ligand binding characteristics and aggregation behavior of purified cow's milk folate binding protein depends on the presence of amphiphatic substances including cholesterol, phospholipids, and synthetic detergents
    Jan Holm
    Department of Clinical Chemistry, Central Hospital Herning, DK 7400, Denmark
    Biosci Rep 22:431-41. 2002
    ....
  3. ncbi A combination of cation exchange and ligand-affinity chromatography for purification of two molecular species of the folate binding protein in human milk, one equipped with a hydrophobic glycosyl phosphatidylinositol tail: characterization of hydrophobici
    Jan Holm
    Department of Clinical Chemistry, Central Hospital, Herning, DK 7400, Denmark
    Biosci Rep 22:443-54. 2002
    ....
  4. ncbi Ligand binding characteristics of two molecular forms, one equipped with a hydrophobic glycosyl phosphatidylinositol tail, of the folate binding protein purified from human milk
    Jan Holm
    Department of Clinical Chemistry, Central Hospital Herning, DK 7400, Denmark
    Biosci Rep 22:455-63. 2002
    ....
  5. ncbi Ligand binding and polymerization characteristics of human milk folate binding protein depend on concentration of purified protein and presence of amphiphatic substances
    Jan Holm
    Department of Clinical Chemistry, Central Hospital Herning, DK 7400, Denmark
    Biosci Rep 23:77-85. 2003
    ..The data could have some bearings on observations indicating that naturally occurring amphiphatic substances, cholesterol and phospholipids, are necessary for the important clustering of membrane folate receptors...
  6. ncbi Characterization of a high affinity folate binding protein in porcine serum: ionic charge, concentration-dependent polymerization and ligand binding mechanism
    Jan Holm
    Department of Clinical Chemistry, Hillerod Hospital, DK 3400, Denmark
    Biosci Rep 23:339-51. 2003
    ..The folate analogue N10 methyl folate was a potent and competitive inhibitor of [3H] folate binding to the folate binding protein, and moreover changed the binding type to apparent negative cooperativity...
  7. ncbi Megalin binds and mediates cellular internalization of folate binding protein
    Henrik Birn
    Department of Cell Biology, Institute of Anatomy, University of Aarhus, Denmark
    FEBS J 272:4423-30. 2005
    ..Megalin is expressed in several absorptive epithelia, including intestine and kidney proximal tubule, and thus the present findings provide a mechanism for intestinal and renal endocytic uptake of soluble FBP...
  8. ncbi A minor fraction of a high-affinity folate binding protein from the epididymis is associated with membranous vesicles and spermatozoa in human semen
    Johan Malm
    Division of Clinical Chemistry, Department of Laboratory Medicine, Lund University, Malmo University Hospital, 205 02 Malmo, Sweden
    Int J Androl 28:267-74. 2005
    ..FBP could have a bacteriostatic function by depriving folate-requiring bacteria of folate and/or ascertain a normal DNA replication subsequent to fertilization by vectorial transfer of folate to the inner compartment of the spermatozoa...
  9. ncbi A novel specific heparin-binding activity of bovine folate-binding protein characterized by capillary electrophoresis
    Niels H H Heegaard
    Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark
    Electrophoresis 27:1122-7. 2006
    ....
  10. ncbi Application of near-infrared and Fourier transform infrared spectroscopy in the characterization of ligand-induced conformation changes in folate binding protein purified from bovine milk: influence of buffer type and pH
    Susanne W Bruun
    Biochemistry and Nutrition Group, BioCentrum DTU, Technical University of Denmark, Kgs Lyngby, Denmark
    Appl Spectrosc 60:737-46. 2006
    ..While the secondary structure changes are revealed by FT-IR, the tertiary and quaternary structure changes are reflected in the NIR spectra, although the general influence of the latter changes on the NIR spectra remains to be confirmed...
  11. ncbi Stopped-flow kinetic studies of the interaction of bovine folate binding protein (FBP) and folate
    Ulla Christensen
    Department of Chemistry, University of Copenhagen, Universitetsparken 5, Copenhagen, DK 2100, Denmark
    Biosci Rep 26:291-9. 2006
    ..4 in three steps. Changes of fluorescence spectra at equilibrium were used to estimate the overall binding constants. Comparative studies on the binding of folate to human albumin are also reported...