Research Topics
| Gabor VajtaSummaryAffiliation: Danish Institute of Agricultural Sciences Country: Denmark Publications
| Collaborators
|
Detail Information
Publications
Open Pulled Straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryosG Vajta
Embryo Technology Center, Danish Institute of Agricultural Sciences, Tjele
Mol Reprod Dev 51:53-8. 1998..The OPS vitrification offers a new way to solve basic problems of reproductive cryobiology and may have practical impact on animal biotechnology and human assisted reproduction...- Somatic cell nuclear transfer in its first and second decades: successes, setbacks, paradoxes and perspectivesGabor Vajta
Institute of Genetics and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, Blichers alle 20, Postboks 50, DK 8830 Tjele, Denmark
Reprod Biomed Online 15:582-90. 2007..Just one fulfilled goal out of the many promises of SCNT would justify the invested efforts... - Handmade cloning: the future way of nuclear transfer?Gabor Vajta
Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, DK 8830 Tjele, Denmark
Trends Biotechnol 25:250-3. 2007..Additionally, HMC offers unique possibilities for the automation of somatic cell nuclear transfer... - Somatic cell nuclear transfer in pigs: recent achievements and future possibilitiesGabor Vajta
Population Genetics and Embryology, Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, DK 8830 Tjele, Denmark
Reprod Fertil Dev 19:403-23. 2007.... - Are programmable freezers still needed in the embryo laboratory? Review on vitrificationGabor Vajta
Population Genetics and Embryology, Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Foulum, DK 8830 Tjele, Denmark
Reprod Biomed Online 12:779-96. 2006..The opinion of the authors is that vitrification is the future of cryopreservation. The public have the final say in whether they want and allow this future to arrive... - Science and technology of farm animal cloning: state of the artGabor Vajta
Population Genetics and Embryology, Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Foulum, DK 8830 Tjele, Denmark
Anim Reprod Sci 92:211-30. 2006..We do not intend to give an exhaustive review of the all the literature available; instead we pinpoint issues and events pivotal to the development of current farm animal cloning practices and their possible applications... - Hand-made cloning approach: potentials and limitationsG Vajta
Section of Reproductive Biology, Department of Animal Breeding and Genetics, Danish Institute of Agricultural Sciences, DK 8830 Tjele, Denmark
Reprod Fertil Dev 17:97-112. 2005.... - Production of a healthy calf by somatic cell nuclear transfer without micromanipulators and carbon dioxide incubators using the Handmade Cloning (HMC) and the Submarine Incubation System (SIS)Gabor Vajta
Reproductive Biology, Department of Animal Breeding and Genetics, Danish Institute of Agricultural Sciences, Tjele 8830, Denmark
Theriogenology 62:1465-72. 2004..The healthy 6-month-old heifer, the first cloned animal of Africa, is living evidence that nuclear transfer technology may be successfully used under basic laboratory conditions... - Rapid growth and elongation of bovine blastocysts in vitro in a three-dimensional gel systemGabor Vajta
Section of Reproductive Biology, Department of Animal Breeding and Genetics, Danish Institute of Agricultural Sciences, Research Centre Foulum, DK 8830 Tjele, Denmark
Theriogenology 62:1253-63. 2004..The established system was suitable to induce in vitro elongation, rapid growth and further differentiation, and may have considerable theoretical and practical value for studies of development and differentiation of bovine embryos... - Handmade somatic cell cloning in cattle: analysis of factors contributing to high efficiency in vitroGabor Vajta
Department of Animal Breeding and Genetics, Danish Institute of Agricultural Sciences, DK 8830 Tjele, Denmark
Biol Reprod 68:571-8. 2003..The improved handmade somatic cell nuclear transfer method may become a useful technology as a simple, inexpensive, and efficient alternative to traditional somatic cell nuclear transfer... - Osmotic stress induced by sodium chloride, sucrose or trehalose improves cryotolerance and developmental competence of porcine oocytesLin Lin
Population Genetics and Embryology, Department of Genetics, Faculty of Agricultural Sciences, University of Aarhus, DK 8830 Tjele, Denmark
Reprod Fertil Dev 21:338-44. 2009..In conclusion, treatment of porcine oocytes with osmotic stress improved developmental competence after vitrification combined with parthenogenetic activation, as well as after SCNT... - Comparison of efficiency of open pulled straw (OPS) and Cryotop vitrification for cryopreservation of in vitro matured pig oocytesYing Liu
Institute of Genetics and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, Denmark
Cryo Letters 29:315-20. 2008..6, 1,65 and 0.6 percent, respectively). Our results indicate that for cryopreservation of some highly sensitive biological specimen including porcine oocytes Cryotop vitrification is superior to the OPS technique... 
High hydrostatic pressure treatment of porcine oocytes induces parthenogenetic activationLin Lin
Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, Aarhus University, Tjele, Denmark
Cell Reprogram 12:475-80. 2010..In conclusion, parthenogenetic activation could be induced by HHP in certain holding media with low or zero Ca(2+) content. Further experiments are needed to identify the exact mechanisms of activation...- Increased blastocyst formation of cloned porcine embryos produced with donor cells pre-treated with Xenopus egg extract and/or digitoninYing Liu
Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, Aarhus University, DK 8830 Tjele, Denmark
Zygote 20:61-6. 2012..Thus, pre-treatment of donor cells using permeabilizing treatment followed by re-sealing and in vitro culture for few days could be a simple way to improve the efficiency of porcine cloning... - Piglets born from vitrified cloned blastocysts produced with a simplified method of delipation and nuclear transferYutao Du
Population Genetics and Embryology, Institute of Genetics and Biotechnology, University of Aarhus, Tjele, Denmark
Cloning Stem Cells 9:469-76. 2007..One recipient became pregnant and delivered six piglets. In conclusion, our simplified delipation and SCNT procedure resulted in viable piglets after vitrification and embryo transfer at the blastocyst stage... - High hydrostatic pressure treatment of porcine oocytes before handmade cloning improves developmental competence and cryosurvivalYutao Du
Population Genetics and Embryology, Institute of Genetics and Biotechnology, University of Aarhus, Tjele, Denmark
Cloning Stem Cells 10:325-30. 2008.... - Elevated NaCl concentration improves cryotolerance and developmental competence of porcine oocytesLin Lin
Population Genetics and Embryology, Institute of Genetics and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, DK 8830 Tjele, Denmark
Reprod Biomed Online 18:360-6. 2009..Further experiments are required to investigate in-vivo consequences, and the effect on gametes and embryos of different mammalian species... - Highly efficient and reliable chemically assisted enucleation method for handmade cloning in cattleGabor Vajta
Population Genetics and Embryology, Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, DK 8830 Tjele, Denmark
Reprod Fertil Dev 17:791-7. 2005..Moreover, expensive equipment (inverted fluorescence microscope) and a potentially harmful step (staining and ultraviolet illumination) can be eliminated from the HMC procedure without compromising the high in vitro efficiency... - Cell colony formation induced by Xenopus egg extract as a marker for improvement of cloned blastocyst formation in the pigYing Liu
Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, Aarhus University, Aarhus, Denmark
Cell Reprogram 13:521-6. 2011..In conclusion, assessment of cell colony formation may be used as selection marker for Xenopus egg extract used for pretreatment of donor cells prior to cloning... - Handmade somatic cell cloning in cattleGabor Vajta
Department of Animal Breeding and Genetics, Danish Institute of Agricultural Sciences, Tjele, Denmark
Methods Mol Biol 348:183-96. 2006..The method may become a useful tool for both experimental and commercial somatic cell cloning because it allows for standardization of procedures and provides the possibility of automation... - An epigenetic modifier results in improved in vitro blastocyst production after somatic cell nuclear transferYunhai Zhang
Section of Population Genetics and Embryology, Department of Genetic and Biotechnology, Danish Institute of Agricultural Sciences, Tjele, Denmark
Cloning Stem Cells 9:357-63. 2007..Our data demonstrate that TSA treatment after somatic cell nuclear transfer in the pig can significantly improve the in vitro blastocyst production... - Pig transgenesis by Sleeping Beauty DNA transpositionJannik E Jakobsen
Department of Human Genetics, University of Aarhus, Wilh Meyers Allé 1240, 8000, Aarhus C, Denmark
Transgenic Res 20:533-45. 2011.... - Improving cryopreservation systemsGabor Vajta
Population Genetics and Embryology, Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Foulum, DK 8830 Tjele, Denmark
Theriogenology 65:236-44. 2006.... - Efficient in vitro production of porcine blastocysts by handmade cloning with a combined electrical and chemical activationP M Kragh
Population Genetics and Embryology, Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, DK 8830 Tjele, Denmark
Theriogenology 64:1536-45. 2005..Thus, the combined electrical and chemical activation procedure resulted in efficient blastocyst development in the handmade cloning technique... - High overall in vitro efficiency of porcine handmade cloning (HMC) combining partial zona digestion and oocyte trisection with sequential cultureY Du
Section of Population Genetics and Embryology, Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Tjele, Denmark
Cloning Stem Cells 7:199-205. 2005..Our results prove that HMC in pigs may result in high in vitro efficiency up until the blastocyst stage. In vivo developmental competence will be confirmed in embryo transfer experiments... - Production of transgenic porcine blastocysts by hand-made cloningP M Kragh
Section of Reproductive Biology, Department of Animal Breeding and Genetics, Danish Institute of Agricultural Sciences, Tjele, Denmark
Reprod Fertil Dev 16:315-8. 2004..0 +/- 0.8%. Enhanced green fluorescent protein was expressed in all cells of all eight developing blastocysts. Efforts are now directed towards the production of offspring from such transgenic NT blastocysts... - High in vitro development after somatic cell nuclear transfer and trichostatin A treatment of reconstructed porcine embryosJ Li
Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, Tjele DK 8830, Denmark
Theriogenology 70:800-8. 2008..Our data demonstrate that TSA treatment after HMC in pigs may affect reprogramming of the somatic genome resulting in higher in vitro embryo development, and enable full-term in vivo development... - Efficiency of two enucleation methods connected to handmade cloning to produce transgenic porcine embryosJ Li
Section of Population Genetics and Embryology, Department of Genetic and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, Tjele, Denmark
Reprod Domest Anim 44:122-7. 2009..OHE enucleation method seems to be a potential superior alternative method used for somatic cell nuclear transfer (SCNT) with transgenic fibroblast cells... - Chemically assisted handmade enucleation of porcine oocytesJ Li
Section of Population Genetics and Embryology, Department of Genetics and Biotechnology, Welfare and Nutrition, Danish Institute of Agricultural Sciences, Research Center Foulum, Tjele, Denmark
Cloning Stem Cells 8:241-50. 2006..In conclusion, chemically assisted handmade enucleation seems to be a simpler and potentially superior alternative to more conventional methods used for somatic cell nuclear transfer in pigs... - High hydrostatic pressure: a new way to improve in vitro developmental competence of porcine matured oocytes after vitrificationY Du
Population Genetics and Embryology, Faculty of Agricultural Sciences, Institute of Genetics and Biotechnology, University of Aarhus, DK 800 Aarhus, Denmark
Reproduction 135:13-7. 2008..The HHP pretreatment will be tested as a means to improve survival and developmental competence at different developmental stages in different species including humans... - Effect of two activation treatments and age of blastomere karyoplasts on in vitro development of bovine nuclear transfer embryosP J Booth
Section for Reproductive Biology, Department of Animal Breeding and Genetics, Danish Institute of Agricultural Sciences, 8830 Tjele, Denmark
Mol Reprod Dev 60:377-83. 2001..In conclusion, the results indicate that although activation of oocytes with DMAP can produce a higher percentage of blastocysts, CHX activation is superior for use in nuclear transfer... - New method for culture of zona-included or zona-free embryos: the Well of the Well (WOW) systemG Vajta
Embryo Technology Center, Danish Institute of Agricultural Sciences, Research Centre Foulum, Tjele, Denmark
Mol Reprod Dev 55:256-64. 2000..Apart from its theoretical value in revealing the role of different factors influencing embryo development in vitro, the WOW system may have immediate practical consequences in certain areas of mammalian embryo production... - Simplified cryopreservation of porcine cloned blastocystsY Du
Section of Population Genetics and Embryology, Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, DK 8830 Tjele, Denmark
Cryobiology 54:181-7. 2007..Further experiments are required to assess the in vivo developmental competence of the cloned-vitrified embryos... - Piglets born from handmade cloning, an innovative cloning method without micromanipulationY Du
Population Genetics and Embryology, Insitute of Genetics and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, DK 8830 Tjele, Denmark
Theriogenology 68:1104-10. 2007..Future comparative examinations are required to prove the value of the new technique for large-scale application... - Full-term development of nuclear transfer calves produced from open-pulled straw (OPS) vitrified cytoplasts: work in progressP J Booth
Embryo Technology Center, Danish Institute of Agricultural Sciences
Theriogenology 51:999-1006. 1999..These data demonstrate that open pulled straw-vitrified cytoplasts are capable of supporting full-term development of nuclear transfer embryos...