Gabor Vajta

Summary

Affiliation: Danish Institute of Agricultural Sciences
Country: Denmark

Publications

  1. ncbi Open Pulled Straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos
    G Vajta
    Embryo Technology Center, Danish Institute of Agricultural Sciences, Tjele
    Mol Reprod Dev 51:53-8. 1998
  2. ncbi Somatic cell nuclear transfer in its first and second decades: successes, setbacks, paradoxes and perspectives
    Gabor Vajta
    Institute of Genetics and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, Blichers alle 20, Postboks 50, DK 8830 Tjele, Denmark
    Reprod Biomed Online 15:582-90. 2007
  3. ncbi Handmade cloning: the future way of nuclear transfer?
    Gabor Vajta
    Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, DK 8830 Tjele, Denmark
    Trends Biotechnol 25:250-3. 2007
  4. ncbi Somatic cell nuclear transfer in pigs: recent achievements and future possibilities
    Gabor Vajta
    Population Genetics and Embryology, Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, DK 8830 Tjele, Denmark
    Reprod Fertil Dev 19:403-23. 2007
  5. ncbi Are programmable freezers still needed in the embryo laboratory? Review on vitrification
    Gabor Vajta
    Population Genetics and Embryology, Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Foulum, DK 8830 Tjele, Denmark
    Reprod Biomed Online 12:779-96. 2006
  6. ncbi Science and technology of farm animal cloning: state of the art
    Gabor Vajta
    Population Genetics and Embryology, Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Foulum, DK 8830 Tjele, Denmark
    Anim Reprod Sci 92:211-30. 2006
  7. ncbi Hand-made cloning approach: potentials and limitations
    G Vajta
    Section of Reproductive Biology, Department of Animal Breeding and Genetics, Danish Institute of Agricultural Sciences, DK 8830 Tjele, Denmark
    Reprod Fertil Dev 17:97-112. 2005
  8. ncbi Production of a healthy calf by somatic cell nuclear transfer without micromanipulators and carbon dioxide incubators using the Handmade Cloning (HMC) and the Submarine Incubation System (SIS)
    Gabor Vajta
    Reproductive Biology, Department of Animal Breeding and Genetics, Danish Institute of Agricultural Sciences, Tjele 8830, Denmark
    Theriogenology 62:1465-72. 2004
  9. ncbi Rapid growth and elongation of bovine blastocysts in vitro in a three-dimensional gel system
    Gabor Vajta
    Section of Reproductive Biology, Department of Animal Breeding and Genetics, Danish Institute of Agricultural Sciences, Research Centre Foulum, DK 8830 Tjele, Denmark
    Theriogenology 62:1253-63. 2004
  10. ncbi Handmade somatic cell cloning in cattle: analysis of factors contributing to high efficiency in vitro
    Gabor Vajta
    Department of Animal Breeding and Genetics, Danish Institute of Agricultural Sciences, DK 8830 Tjele, Denmark
    Biol Reprod 68:571-8. 2003

Detail Information

Publications35

  1. ncbi Open Pulled Straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos
    G Vajta
    Embryo Technology Center, Danish Institute of Agricultural Sciences, Tjele
    Mol Reprod Dev 51:53-8. 1998
    ..The OPS vitrification offers a new way to solve basic problems of reproductive cryobiology and may have practical impact on animal biotechnology and human assisted reproduction...
  2. ncbi Somatic cell nuclear transfer in its first and second decades: successes, setbacks, paradoxes and perspectives
    Gabor Vajta
    Institute of Genetics and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, Blichers alle 20, Postboks 50, DK 8830 Tjele, Denmark
    Reprod Biomed Online 15:582-90. 2007
    ..Just one fulfilled goal out of the many promises of SCNT would justify the invested efforts...
  3. ncbi Handmade cloning: the future way of nuclear transfer?
    Gabor Vajta
    Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, DK 8830 Tjele, Denmark
    Trends Biotechnol 25:250-3. 2007
    ..Additionally, HMC offers unique possibilities for the automation of somatic cell nuclear transfer...
  4. ncbi Somatic cell nuclear transfer in pigs: recent achievements and future possibilities
    Gabor Vajta
    Population Genetics and Embryology, Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, DK 8830 Tjele, Denmark
    Reprod Fertil Dev 19:403-23. 2007
    ....
  5. ncbi Are programmable freezers still needed in the embryo laboratory? Review on vitrification
    Gabor Vajta
    Population Genetics and Embryology, Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Foulum, DK 8830 Tjele, Denmark
    Reprod Biomed Online 12:779-96. 2006
    ..The opinion of the authors is that vitrification is the future of cryopreservation. The public have the final say in whether they want and allow this future to arrive...
  6. ncbi Science and technology of farm animal cloning: state of the art
    Gabor Vajta
    Population Genetics and Embryology, Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Foulum, DK 8830 Tjele, Denmark
    Anim Reprod Sci 92:211-30. 2006
    ..We do not intend to give an exhaustive review of the all the literature available; instead we pinpoint issues and events pivotal to the development of current farm animal cloning practices and their possible applications...
  7. ncbi Hand-made cloning approach: potentials and limitations
    G Vajta
    Section of Reproductive Biology, Department of Animal Breeding and Genetics, Danish Institute of Agricultural Sciences, DK 8830 Tjele, Denmark
    Reprod Fertil Dev 17:97-112. 2005
    ....
  8. ncbi Production of a healthy calf by somatic cell nuclear transfer without micromanipulators and carbon dioxide incubators using the Handmade Cloning (HMC) and the Submarine Incubation System (SIS)
    Gabor Vajta
    Reproductive Biology, Department of Animal Breeding and Genetics, Danish Institute of Agricultural Sciences, Tjele 8830, Denmark
    Theriogenology 62:1465-72. 2004
    ..The healthy 6-month-old heifer, the first cloned animal of Africa, is living evidence that nuclear transfer technology may be successfully used under basic laboratory conditions...
  9. ncbi Rapid growth and elongation of bovine blastocysts in vitro in a three-dimensional gel system
    Gabor Vajta
    Section of Reproductive Biology, Department of Animal Breeding and Genetics, Danish Institute of Agricultural Sciences, Research Centre Foulum, DK 8830 Tjele, Denmark
    Theriogenology 62:1253-63. 2004
    ..The established system was suitable to induce in vitro elongation, rapid growth and further differentiation, and may have considerable theoretical and practical value for studies of development and differentiation of bovine embryos...
  10. ncbi Handmade somatic cell cloning in cattle: analysis of factors contributing to high efficiency in vitro
    Gabor Vajta
    Department of Animal Breeding and Genetics, Danish Institute of Agricultural Sciences, DK 8830 Tjele, Denmark
    Biol Reprod 68:571-8. 2003
    ..The improved handmade somatic cell nuclear transfer method may become a useful technology as a simple, inexpensive, and efficient alternative to traditional somatic cell nuclear transfer...
  11. ncbi Osmotic stress induced by sodium chloride, sucrose or trehalose improves cryotolerance and developmental competence of porcine oocytes
    Lin Lin
    Population Genetics and Embryology, Department of Genetics, Faculty of Agricultural Sciences, University of Aarhus, DK 8830 Tjele, Denmark
    Reprod Fertil Dev 21:338-44. 2009
    ..In conclusion, treatment of porcine oocytes with osmotic stress improved developmental competence after vitrification combined with parthenogenetic activation, as well as after SCNT...
  12. ncbi Comparison of efficiency of open pulled straw (OPS) and Cryotop vitrification for cryopreservation of in vitro matured pig oocytes
    Ying Liu
    Institute of Genetics and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, Denmark
    Cryo Letters 29:315-20. 2008
    ..6, 1,65 and 0.6 percent, respectively). Our results indicate that for cryopreservation of some highly sensitive biological specimen including porcine oocytes Cryotop vitrification is superior to the OPS technique...
  13. doi High hydrostatic pressure treatment of porcine oocytes induces parthenogenetic activation
    Lin Lin
    Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, Aarhus University, Tjele, Denmark
    Cell Reprogram 12:475-80. 2010
    ..In conclusion, parthenogenetic activation could be induced by HHP in certain holding media with low or zero Ca(2+) content. Further experiments are needed to identify the exact mechanisms of activation...
  14. doi Increased blastocyst formation of cloned porcine embryos produced with donor cells pre-treated with Xenopus egg extract and/or digitonin
    Ying Liu
    Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, Aarhus University, DK 8830 Tjele, Denmark
    Zygote 20:61-6. 2012
    ..Thus, pre-treatment of donor cells using permeabilizing treatment followed by re-sealing and in vitro culture for few days could be a simple way to improve the efficiency of porcine cloning...
  15. ncbi Piglets born from vitrified cloned blastocysts produced with a simplified method of delipation and nuclear transfer
    Yutao Du
    Population Genetics and Embryology, Institute of Genetics and Biotechnology, University of Aarhus, Tjele, Denmark
    Cloning Stem Cells 9:469-76. 2007
    ..One recipient became pregnant and delivered six piglets. In conclusion, our simplified delipation and SCNT procedure resulted in viable piglets after vitrification and embryo transfer at the blastocyst stage...
  16. doi High hydrostatic pressure treatment of porcine oocytes before handmade cloning improves developmental competence and cryosurvival
    Yutao Du
    Population Genetics and Embryology, Institute of Genetics and Biotechnology, University of Aarhus, Tjele, Denmark
    Cloning Stem Cells 10:325-30. 2008
    ....
  17. ncbi Highly efficient and reliable chemically assisted enucleation method for handmade cloning in cattle
    Gabor Vajta
    Population Genetics and Embryology, Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, DK 8830 Tjele, Denmark
    Reprod Fertil Dev 17:791-7. 2005
    ..Moreover, expensive equipment (inverted fluorescence microscope) and a potentially harmful step (staining and ultraviolet illumination) can be eliminated from the HMC procedure without compromising the high in vitro efficiency...
  18. ncbi Elevated NaCl concentration improves cryotolerance and developmental competence of porcine oocytes
    Lin Lin
    Population Genetics and Embryology, Institute of Genetics and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, DK 8830 Tjele, Denmark
    Reprod Biomed Online 18:360-6. 2009
    ..Further experiments are required to investigate in-vivo consequences, and the effect on gametes and embryos of different mammalian species...
  19. doi Cell colony formation induced by Xenopus egg extract as a marker for improvement of cloned blastocyst formation in the pig
    Ying Liu
    Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, Aarhus University, Aarhus, Denmark
    Cell Reprogram 13:521-6. 2011
    ..In conclusion, assessment of cell colony formation may be used as selection marker for Xenopus egg extract used for pretreatment of donor cells prior to cloning...
  20. ncbi Handmade somatic cell cloning in cattle
    Gabor Vajta
    Department of Animal Breeding and Genetics, Danish Institute of Agricultural Sciences, Tjele, Denmark
    Methods Mol Biol 348:183-96. 2006
    ..The method may become a useful tool for both experimental and commercial somatic cell cloning because it allows for standardization of procedures and provides the possibility of automation...
  21. ncbi An epigenetic modifier results in improved in vitro blastocyst production after somatic cell nuclear transfer
    Yunhai Zhang
    Section of Population Genetics and Embryology, Department of Genetic and Biotechnology, Danish Institute of Agricultural Sciences, Tjele, Denmark
    Cloning Stem Cells 9:357-63. 2007
    ..Our data demonstrate that TSA treatment after somatic cell nuclear transfer in the pig can significantly improve the in vitro blastocyst production...
  22. doi Pig transgenesis by Sleeping Beauty DNA transposition
    Jannik E Jakobsen
    Department of Human Genetics, University of Aarhus, Wilh Meyers Allé 1240, 8000, Aarhus C, Denmark
    Transgenic Res 20:533-45. 2011
    ....
  23. ncbi Improving cryopreservation systems
    Gabor Vajta
    Population Genetics and Embryology, Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Research Centre Foulum, DK 8830 Tjele, Denmark
    Theriogenology 65:236-44. 2006
    ....
  24. ncbi Efficient in vitro production of porcine blastocysts by handmade cloning with a combined electrical and chemical activation
    P M Kragh
    Population Genetics and Embryology, Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, DK 8830 Tjele, Denmark
    Theriogenology 64:1536-45. 2005
    ..Thus, the combined electrical and chemical activation procedure resulted in efficient blastocyst development in the handmade cloning technique...
  25. ncbi High overall in vitro efficiency of porcine handmade cloning (HMC) combining partial zona digestion and oocyte trisection with sequential culture
    Y Du
    Section of Population Genetics and Embryology, Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Tjele, Denmark
    Cloning Stem Cells 7:199-205. 2005
    ..Our results prove that HMC in pigs may result in high in vitro efficiency up until the blastocyst stage. In vivo developmental competence will be confirmed in embryo transfer experiments...
  26. ncbi Production of transgenic porcine blastocysts by hand-made cloning
    P M Kragh
    Section of Reproductive Biology, Department of Animal Breeding and Genetics, Danish Institute of Agricultural Sciences, Tjele, Denmark
    Reprod Fertil Dev 16:315-8. 2004
    ..0 +/- 0.8%. Enhanced green fluorescent protein was expressed in all cells of all eight developing blastocysts. Efforts are now directed towards the production of offspring from such transgenic NT blastocysts...
  27. ncbi High in vitro development after somatic cell nuclear transfer and trichostatin A treatment of reconstructed porcine embryos
    J Li
    Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, Tjele DK 8830, Denmark
    Theriogenology 70:800-8. 2008
    ..Our data demonstrate that TSA treatment after HMC in pigs may affect reprogramming of the somatic genome resulting in higher in vitro embryo development, and enable full-term in vivo development...
  28. doi Efficiency of two enucleation methods connected to handmade cloning to produce transgenic porcine embryos
    J Li
    Section of Population Genetics and Embryology, Department of Genetic and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, Tjele, Denmark
    Reprod Domest Anim 44:122-7. 2009
    ..OHE enucleation method seems to be a potential superior alternative method used for somatic cell nuclear transfer (SCNT) with transgenic fibroblast cells...
  29. ncbi Chemically assisted handmade enucleation of porcine oocytes
    J Li
    Section of Population Genetics and Embryology, Department of Genetics and Biotechnology, Welfare and Nutrition, Danish Institute of Agricultural Sciences, Research Center Foulum, Tjele, Denmark
    Cloning Stem Cells 8:241-50. 2006
    ..In conclusion, chemically assisted handmade enucleation seems to be a simpler and potentially superior alternative to more conventional methods used for somatic cell nuclear transfer in pigs...
  30. ncbi High hydrostatic pressure: a new way to improve in vitro developmental competence of porcine matured oocytes after vitrification
    Y Du
    Population Genetics and Embryology, Faculty of Agricultural Sciences, Institute of Genetics and Biotechnology, University of Aarhus, DK 800 Aarhus, Denmark
    Reproduction 135:13-7. 2008
    ..The HHP pretreatment will be tested as a means to improve survival and developmental competence at different developmental stages in different species including humans...
  31. ncbi Effect of two activation treatments and age of blastomere karyoplasts on in vitro development of bovine nuclear transfer embryos
    P J Booth
    Section for Reproductive Biology, Department of Animal Breeding and Genetics, Danish Institute of Agricultural Sciences, 8830 Tjele, Denmark
    Mol Reprod Dev 60:377-83. 2001
    ..In conclusion, the results indicate that although activation of oocytes with DMAP can produce a higher percentage of blastocysts, CHX activation is superior for use in nuclear transfer...
  32. ncbi New method for culture of zona-included or zona-free embryos: the Well of the Well (WOW) system
    G Vajta
    Embryo Technology Center, Danish Institute of Agricultural Sciences, Research Centre Foulum, Tjele, Denmark
    Mol Reprod Dev 55:256-64. 2000
    ..Apart from its theoretical value in revealing the role of different factors influencing embryo development in vitro, the WOW system may have immediate practical consequences in certain areas of mammalian embryo production...
  33. ncbi Simplified cryopreservation of porcine cloned blastocysts
    Y Du
    Section of Population Genetics and Embryology, Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, DK 8830 Tjele, Denmark
    Cryobiology 54:181-7. 2007
    ..Further experiments are required to assess the in vivo developmental competence of the cloned-vitrified embryos...
  34. ncbi Piglets born from handmade cloning, an innovative cloning method without micromanipulation
    Y Du
    Population Genetics and Embryology, Insitute of Genetics and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, DK 8830 Tjele, Denmark
    Theriogenology 68:1104-10. 2007
    ..Future comparative examinations are required to prove the value of the new technique for large-scale application...
  35. ncbi Full-term development of nuclear transfer calves produced from open-pulled straw (OPS) vitrified cytoplasts: work in progress
    P J Booth
    Embryo Technology Center, Danish Institute of Agricultural Sciences
    Theriogenology 51:999-1006. 1999
    ..These data demonstrate that open pulled straw-vitrified cytoplasts are capable of supporting full-term development of nuclear transfer embryos...