Lone Gram

Summary

Affiliation: Danish Institute for Fisheries Research
Country: Denmark

Publications

  1. pmc Inhibition of vibrio anguillarum by Pseudomonas fluorescens AH2, a possible probiotic treatment of fish
    L Gram
    Danish Institute for Fisheries Research, Department of Seafood Research, Technical University of Denmark, DK 2800 Lyngby, Denmark
    Appl Environ Microbiol 65:969-73. 1999
  2. ncbi request reprint Food spoilage--interactions between food spoilage bacteria
    Lone Gram
    Department of Seafood Research, Danish Institute for Fisheries Research, Lyngby
    Int J Food Microbiol 78:79-97. 2002
  3. ncbi request reprint Fish spoilage bacteria--problems and solutions
    Lone Gram
    Department of Seafood Research, Danish Institute for Fisheries Research, Søltofts Plads, c o Technical University of Denmark, Bldg 221, DK 2800 Kgs Lyngby, Denmark
    Curr Opin Biotechnol 13:262-6. 2002
  4. pmc Possible quorum sensing in marine snow bacteria: production of acylated homoserine lactones by Roseobacter strains isolated from marine snow
    Lone Gram
    Department of Seafood Research, Danish Institute for Fisheries Research, DK 2800 Kgs Lyngby, Denmark
    Appl Environ Microbiol 68:4111-6. 2002
  5. pmc Occurrence of Shewanella algae in Danish coastal water and effects of water temperature and culture conditions on its survival
    L Gram
    Danish Institute for Fisheries Research, Department of Seafood Research, Technical University of Denmark, DK 2800 Lyngby, Denmark
    Appl Environ Microbiol 65:3896-900. 1999
  6. pmc Production of acylated homoserine lactones by psychrotrophic members of the Enterobacteriaceae isolated from foods
    L Gram
    Danish Institute for Fisheries Research, Department of Seafood Research, Technical University of Denmark, DK 2800 Lyngby, Denmark
    Appl Environ Microbiol 65:3458-63. 1999
  7. doi request reprint Processing plant persistent strains of Listeria monocytogenes appear to have a lower virulence potential than clinical strains in selected virulence models
    Anne Jensen
    Department of Seafood Research, National Institute of Aquatic Resources, Technical University of Denmark, Søltofts Plads Bldg 221, DK 2800 Kgs Lyngby, Denmark
    Int J Food Microbiol 123:254-61. 2008
  8. pmc Phaeobacter and Ruegeria species of the Roseobacter clade colonize separate niches in a Danish Turbot (Scophthalmus maximus)-rearing farm and antagonize Vibrio anguillarum under different growth conditions
    Cisse Hedegaard Porsby
    National Institute of Aquatic Resources, Technical University of Denmark, Kongens Lyngby, Denmark
    Appl Environ Microbiol 74:7356-64. 2008
  9. pmc Involvement of bacterial quorum-sensing signals in spoilage of bean sprouts
    Maria Rasch
    Danish Institute for Fisheries Research, Department of Seafood Research, Søltofts Plads, c o Technical University of Denmark Bldg 221, DK 2800 Kgs Lyngby, Denmark
    Appl Environ Microbiol 71:3321-30. 2005
  10. doi request reprint Survival and growth of Salmonella and Vibrio in som-fak, a Thai low-salt garlic containing fermented fish product
    Nete Bernbom
    DTU Aqua, National Institute of Aquatic Resources, Technical University of Denmark, Søltofts Plads, Building 221, 2800 Kgs Lyngby, Denmark
    Int J Food Microbiol 134:223-9. 2009

Detail Information

Publications77

  1. pmc Inhibition of vibrio anguillarum by Pseudomonas fluorescens AH2, a possible probiotic treatment of fish
    L Gram
    Danish Institute for Fisheries Research, Department of Seafood Research, Technical University of Denmark, DK 2800 Lyngby, Denmark
    Appl Environ Microbiol 65:969-73. 1999
    ....
  2. ncbi request reprint Food spoilage--interactions between food spoilage bacteria
    Lone Gram
    Department of Seafood Research, Danish Institute for Fisheries Research, Lyngby
    Int J Food Microbiol 78:79-97. 2002
    ..In particular, we report for the first time the widespread occurrence of N-acyl homoserine lactones (AHL) in stored and spoiling fresh foods and we discuss the potential implications for spoilage and food preservation...
  3. ncbi request reprint Fish spoilage bacteria--problems and solutions
    Lone Gram
    Department of Seafood Research, Danish Institute for Fisheries Research, Søltofts Plads, c o Technical University of Denmark, Bldg 221, DK 2800 Kgs Lyngby, Denmark
    Curr Opin Biotechnol 13:262-6. 2002
    ..Combining microbial ecology, molecular techniques, analytical chemistry, sensory analysis and mathematical modelling allows us to characterise the SSOs and to develop methods to determine, predict and extend the shelf life of products...
  4. pmc Possible quorum sensing in marine snow bacteria: production of acylated homoserine lactones by Roseobacter strains isolated from marine snow
    Lone Gram
    Department of Seafood Research, Danish Institute for Fisheries Research, DK 2800 Kgs Lyngby, Denmark
    Appl Environ Microbiol 68:4111-6. 2002
    ..g., in the marine snow community...
  5. pmc Occurrence of Shewanella algae in Danish coastal water and effects of water temperature and culture conditions on its survival
    L Gram
    Danish Institute for Fisheries Research, Department of Seafood Research, Technical University of Denmark, DK 2800 Lyngby, Denmark
    Appl Environ Microbiol 65:3896-900. 1999
    ..It was hypothesized that S. algae would survive cold exposure better if in the biofilm state; however, culturable counts from S. algae biofilms decreased as rapidly as did counts of planktonic cells...
  6. pmc Production of acylated homoserine lactones by psychrotrophic members of the Enterobacteriaceae isolated from foods
    L Gram
    Danish Institute for Fisheries Research, Department of Seafood Research, Technical University of Denmark, DK 2800 Lyngby, Denmark
    Appl Environ Microbiol 65:3458-63. 1999
    ..This level of Enterobacteriaceae is often found in lightly preserved foods, and AHL-mediated gene regulation may play a role in bacteria associated with food spoilage or food toxicity...
  7. doi request reprint Processing plant persistent strains of Listeria monocytogenes appear to have a lower virulence potential than clinical strains in selected virulence models
    Anne Jensen
    Department of Seafood Research, National Institute of Aquatic Resources, Technical University of Denmark, Søltofts Plads Bldg 221, DK 2800 Kgs Lyngby, Denmark
    Int J Food Microbiol 123:254-61. 2008
    ....
  8. pmc Phaeobacter and Ruegeria species of the Roseobacter clade colonize separate niches in a Danish Turbot (Scophthalmus maximus)-rearing farm and antagonize Vibrio anguillarum under different growth conditions
    Cisse Hedegaard Porsby
    National Institute of Aquatic Resources, Technical University of Denmark, Kongens Lyngby, Denmark
    Appl Environ Microbiol 74:7356-64. 2008
    ..displayed antibacterial activity under a broader range of growth conditions than did Phaeobacter strain 27-4, these organisms may hold greater promise as fish probiotic organisms...
  9. pmc Involvement of bacterial quorum-sensing signals in spoilage of bean sprouts
    Maria Rasch
    Danish Institute for Fisheries Research, Department of Seafood Research, Søltofts Plads, c o Technical University of Denmark Bldg 221, DK 2800 Kgs Lyngby, Denmark
    Appl Environ Microbiol 71:3321-30. 2005
    ....
  10. doi request reprint Survival and growth of Salmonella and Vibrio in som-fak, a Thai low-salt garlic containing fermented fish product
    Nete Bernbom
    DTU Aqua, National Institute of Aquatic Resources, Technical University of Denmark, Søltofts Plads, Building 221, 2800 Kgs Lyngby, Denmark
    Int J Food Microbiol 134:223-9. 2009
    ..However, our results also suggest that the use of garlic fermenting starter cultures in combination with garlic could improve safety of fermented fish products...
  11. ncbi request reprint Profiling of acylated homoserine lactones of Vibrio anguillarum in vitro and in vivo: Influence of growth conditions and serotype
    Christiane Buchholtz
    Department of Seafood Research, Danish Institute for Fisheries Research, Søltofts Plads, DTU bldg 221, DK 2800 Kgs Lyngby, Denmark
    Syst Appl Microbiol 29:433-45. 2006
    ..For future studies of QS systems and the possible specific interference with expression of virulence factors, in vitro cultures should be optimised to reflect the in vivo situation...
  12. ncbi request reprint An inhibitor of bacterial quorum sensing reduces mortalities caused by Vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum)
    Maria Rasch
    Danish Institute for Fisheries Research, Department of Seafood Research, Søltofts Plads, c o Technical University of Denmark, Lyngby, Denmark
    Syst Appl Microbiol 27:350-9. 2004
    ..However, they also underline the need for development of novel, less toxic QSI compounds and the need for understanding the exact mechanism(s) of action...
  13. pmc Seasonal incidence of autochthonous antagonistic Roseobacter spp. and Vibrionaceae strains in a turbot larva (Scophthalmus maximus) rearing system
    Mette Hjelm
    Department of Seafood Research, Danish Institute for Fisheries Research, Kongens Lyngby, Denmark
    Appl Environ Microbiol 70:7288-94. 2004
    ..strains were, in particular, isolated from the larval tank walls, and it may be possible to establish an antagonistic, beneficial microflora in the rearing environment of turbot larvae and thereby limit survival of pathogenic bacteria...
  14. ncbi request reprint A processing plant persistent strain of Listeria monocytogenes crosses the fetoplacental barrier in a pregnant guinea pig model
    Anne Jensen
    Department of Seafood Research, National Institute of Aquatic Resources, Technical University of Denmark, Søltofts Plads Building 221, DK 2800 Kgs Lyngby, Denmark
    J Food Prot 71:1028-34. 2008
    ..Furthermore, we demonstrate that although information can be gained from model virulence assays, assessment of the virulence potential of a strain may require more complex hosts...
  15. ncbi request reprint Antibacterial activity of marine culturable bacteria collected from a global sampling of ocean surface waters and surface swabs of marine organisms
    Lone Gram
    National Institute of Aquatic Resources, Technical University of Denmark, Søltofts Plads Building 221, 2800 Kgs Lyngby, Denmark
    Mar Biotechnol (NY) 12:439-51. 2010
    ..Ruegeria strains were isolated from all ocean areas except for Arctic and Antarctic waters and inhibitory activity caused by production of tropodithietic acid...
  16. doi request reprint Influence of processing steps in cold-smoked salmon production on survival and growth of persistent and presumed non-persistent Listeria monocytogenes
    Cisse Hedegaard Porsby
    National Institute of Aquatic Resources, Technical University of Denmark, Søltofts Plads, Bldg 221, DK 2800 Kgs Lyngby, Denmark
    Int J Food Microbiol 122:287-95. 2008
    ..monocytogenes. A persistent strain was no less sensitive to the processing steps than a clinical strain or strain EGD...
  17. pmc Identification of four new agr quorum sensing-interfering cyclodepsipeptides from a marine Photobacterium
    Louise Kjaerulff
    Department of Chemistry, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
    Mar Drugs 11:5051-62. 2013
    ....
  18. ncbi request reprint Influence of food preservation parameters and associated microbiota on production rate, profile and stability of acylated homoserine lactones from food-derived Enterobacteriaceae
    Lars Ravn Flodgaard
    Department of Seafood Research, Danish Institute for Fisheries Research, Søltofts Plads, c o Technical University of Denmark, Bldg 221, DK 2800 Kgs Lyngby, Denmark
    Int J Food Microbiol 84:145-56. 2003
    ..Also, the AHLs produced in foods will be relatively stable and their regulatory impact lasting during storage...
  19. pmc Antibacterial compounds from marine Vibrionaceae isolated on a global expedition
    Matthias Wietz
    National Food Institute, Technical University of Denmark, 2800 Kgs Lyngby, Denmark
    Mar Drugs 8:2946-60. 2010
    ..halotolerans). Despite the isolation of already known antibiotics, our findings show that marine Vibrionaceae are a resource of antibacterial compounds and may have potential for future natural product discovery...
  20. pmc Inhibition of virulence gene expression in Staphylococcus aureus by novel depsipeptides from a marine photobacterium
    Maria Mansson
    Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark, Kgs Lyngby, Denmark
    Mar Drugs 9:2537-52. 2011
    ..aureus strain 8325-4 and the highly virulent, community-acquired strain USA300 (CA-MRSA). To our knowledge, this is the first report of inhibitors of the agr system by a marine bacterium...
  21. ncbi request reprint Prevalence and survival of Listeria monocytogenes in Danish aquatic and fish-processing environments
    Cisse Hedegaard Hansen
    Department of Seafood Research, Danish Institute for Fisheries Research, Søltofts Plads, DTU Building 221, DK 2800 Kgs Lyngby, Denmark
    J Food Prot 69:2113-22. 2006
    ..Therefore, L. monocytogenes in the outer environment associated with Danish fish processing is probably of minor importance to the environment inside a fish production plant...
  22. ncbi request reprint Sodium chloride enhances adherence and aggregation and strain variation influences invasiveness of Listeria monocytogenes strains
    Anne Jensen
    Danish Institute for Fisheries Research, Department of Seafood Research, Søltofts Plads, DTU bldg 221, DK 2800 Kgs Lyngby, Denmark
    J Food Prot 70:592-9. 2007
    ....
  23. pmc Ecology, inhibitory activity, and morphogenesis of a marine antagonistic bacterium belonging to the Roseobacter clade
    Jesper Bartholin Bruhn
    Danish Institute for Fisheries Research, Department of Seafood Research, Søltofts Plads, DTU bldg 221, DK 2800 Kgs Lyngby, Denmark
    Appl Environ Microbiol 71:7263-70. 2005
    ..Using Roseobacter 27-4 as a probiotic culture will require that is be established in stagnant or adhered conditions and, due to the temperature sensitivity of the active compound, constant production must be ensured...
  24. pmc Adaptive evolution of Escherichia coli to an α-peptide/β-peptoid peptidomimetic induces stable resistance
    Line Hein-Kristensen
    Division of Industrial Food Research, National Food Institute, Technical University of Denmark, Kgs Lyngby, Denmark
    PLoS ONE 8:e73620. 2013
    ..Interestingly, this type of resistance result in a concomitant high susceptibility towards plasma, and therefore the present study does not infer additional concern for peptidomimetics as future therapeutics...
  25. ncbi request reprint Selectivity in the potentiation of antibacterial activity of α-peptide/β-peptoid peptidomimetics and antimicrobial peptides by human blood plasma
    Line Hein-Kristensen
    National Food Institute, Technical University of Denmark, Søltofts Plads, Bldg 221, DK 2800 Kgs Lyngby, Denmark Electronic address
    Res Microbiol 164:933-40. 2013
    ..The unexpected enhancement of antibacterial activity of peptidomimetics and AMPs under physiological conditions significantly increases the therapeutic potential of these compounds. ..
  26. ncbi request reprint Quorum sensing signals are produced by Aeromonas salmonicida and quorum sensing inhibitors can reduce production of a potential virulence factor
    Maria Rasch
    Danish Institute for Fisheries Research, Department of Seafood Research, Technical University of Denmark, Søltofts Plads Bldg 221, 2800 Kgs Lyngby, Denmark
    Dis Aquat Organ 78:105-13. 2007
    ..Hence, QSIs can curb virulence in some strains and could potentially be pursued as bacterial disease control measures in aquaculture...
  27. pmc Poor invasion of trophoblastic cells but normal plaque formation in fibroblastic cells despite actA deletion in a group of Listeria monocytogenes strains persisting in some food processing environments
    Anne Holch
    National Institute of Aquatic Resources, Technical University of Denmark, Søltofts Plads, Bldg 221, DK 2800 Kgs Lyngby, Denmark
    Appl Environ Microbiol 76:3391-7. 2010
    ..The RAPD 9 strains may still be regarded as of low virulence with respect to human listeriosis...
  28. ncbi request reprint Potassium lactate combined with sodium diacetate can inhibit growth of Listeria monocytogenes in vacuum-packed cold-smoked salmon and has no adverse sensory effects
    Birte Fonnesbech Vogel
    Department of Seafood Research, Danish Institute for Fisheries Research, Søltofts Plads, DTU Buliding 221, DK 2800 Kgs Lyngby, Denmark
    J Food Prot 69:2134-42. 2006
    ..Sensory analyses revealed that the preservatives did not negatively affect flavor or odor. The combination of PL and SDA is therefore a viable technology for preventing L. monocytogenes growth on cold-smoked salmon...
  29. pmc Inactivation of Vibrio anguillarum by attached and planktonic Roseobacter cells
    Paul W D'Alvise
    National Food Institute, Technical University of Denmark, Søltofts Plads Bldg 221, DK 2800 Kgs Lyngby, Denmark
    Appl Environ Microbiol 76:2366-70. 2010
    ..The effect was likely associated with the production of tropodithietic acid (TDA), as a TDA-negative mutant did not affect survival or growth of V. anguillarum...
  30. pmc Identification of Shewanella baltica as the most important H2S-producing species during iced storage of Danish marine fish
    Birte Fonnesbech Vogel
    Danish Institute for Fisheries Research, Department of Seafood Research, Søltofts Plads, c o Technical University of Denmark, Bldg 221, DK 2800 Kgs Lyngby, Denmark
    Appl Environ Microbiol 71:6689-97. 2005
    ..In conclusion, the spoilage reactions of iced Danish marine fish remain unchanged (i.e., trimethylamine-N-oxide reduction and H2S production); however, the main H2S-producing organism was identified as S. baltica...
  31. ncbi request reprint The microbial ecology of processing equipment in different fish industries-analysis of the microflora during processing and following cleaning and disinfection
    Dorthe Bagge-Ravn
    Department of Seafood Research, Danish Institute for Fisheries Research, Søltofts Plads, c o Technical University of Denmark, Bldg 221, DK 2800 Kgs Lyngby, Denmark
    Int J Food Microbiol 87:239-50. 2003
    ....
  32. doi request reprint Listeria monocytogenes strains encoding premature stop codons in inlA invade mice and guinea pig fetuses in orally dosed dams
    Anne Holch
    National Food Institute, Technical University of Denmark, Søltofts Plads, Bldg 221, DK 2800 Kongens Lyngby, Denmark
    J Med Microbiol 62:1799-806. 2013
    ....
  33. ncbi request reprint Purification and characterisation of an extracellular fructan beta-fructosidase from a Lactobacillus pentosus strain isolated from fermented fish
    Christine Paludan-Müller
    Danish Institute for Fisheries Research, Department of Seafood Research, Søltofts Plads, Technical University of Denmark, Lyngby
    Syst Appl Microbiol 25:13-20. 2002
    ..The enzyme had temperature and pH optima at 25 degrees C and 5.5, respectively. At concentrations of up to 12% NaCl no adverse effect on the enzyme activity was observed...
  34. doi request reprint Industrial disinfectants do not select for resistance in Listeria monocytogenes following long term exposure
    Vicky G Kastbjerg
    National Food Institute, Technical University of Denmark, Søltofts Plads Bldg 221, DK 2800 Kgs Lyngby, Denmark
    Int J Food Microbiol 160:11-5. 2012
    ..Our data are in agreement with the fact that finding strains with high acquired resistance to disinfectants is rare, and that the disinfectants are still efficient for controlling microorganisms such as L. monocytogenes...
  35. pmc Phaeobacter gallaeciensis reduces Vibrio anguillarum in cultures of microalgae and rotifers, and prevents vibriosis in cod larvae
    Paul W D'Alvise
    National Food Institute, Technical University of Denmark, Lyngby, Denmark
    PLoS ONE 7:e43996. 2012
    ..It is concluded that P. gallaeciensis is a promising probiont in marine larviculture and that TDA production likely contributes to its probiotic effect...
  36. doi request reprint Bioactivity, chemical profiling, and 16S rRNA-based phylogeny of Pseudoalteromonas strains collected on a global research cruise
    Nikolaj G Vynne
    National Food Institute, Technical University of Denmark, Søltofts Plads, Bldg 221, 2800, Kgs Lyngby, Denmark
    Mar Biotechnol (NY) 13:1062-73. 2011
    ..Hence, the pigmented, antibacterial Pseudoalteromonas have a niche specificity, and sampling from marine biofilm environments is a strategy for isolating novel marine bacteria that produce antibacterial compounds...
  37. pmc Bias in the Listeria monocytogenes enrichment procedure: lineage 2 strains outcompete lineage 1 strains in University of Vermont selective enrichments
    Jesper Bartholin Bruhn
    Danish Institute for Fisheries Research, Department of Seafood Research, Søltofts Plads, c o Technical University of Denmark, Bldg 221, DK 2800 Kongens Lyngby, Denmark
    Appl Environ Microbiol 71:961-7. 2005
    ..monocytogenes lineages present in a food sample...
  38. pmc Culture conditions of Roseobacter strain 27-4 affect its attachment and biofilm formation as quantified by real-time PCR
    Jesper Bartholin Bruhn
    Department of Seafood Research, Danish Institute for Fisheries Research, Søltofts Plads, DTU bldg 221, DK 2800 Kongens Lyngby, Denmark
    Appl Environ Microbiol 72:3011-5. 2006
    ..We quantified attachment of Roseobacter strain 27-4 using a direct real-time PCR method and demonstrated that the bacteria attached more efficiently to surfaces during static growth than under aerated conditions...
  39. pmc One group of genetically similar Listeria monocytogenes strains frequently dominates and persists in several fish slaughter- and smokehouses
    Gitte Wulff
    Danish Institute for Fisheries Research, Department of Seafood Research, Søltofts Plads, DTU bldg 221, DK 2800 Kgs Lyngby, Denmark
    Appl Environ Microbiol 72:4313-22. 2006
    ..Our study indicates that strains of L. monocytogenes that are genetically very closely related may be especially adapted to colonizing the processing equipment or especially resistant to cleaning and disinfection...
  40. pmc Production of antibacterial compounds and biofilm formation by Roseobacter species are influenced by culture conditions
    Jesper Bartholin Bruhn
    Danish Institute for Fisheries Research, Department of Seafood Research, Soeltofts Plads, DTU bldg 221, DK 2800 Kgs Lyngby, Denmark
    Appl Environ Microbiol 73:442-50. 2007
    ....
  41. pmc Pseudoalteromonas spp. serve as initial bacterial attractants in mesocosms of coastal waters but have subsequent antifouling capacity in mesocosms and when embedded in paint
    Nete Bernbom
    National Food Institute, Technical University of Denmark, Kongens Lyngby, Denmark
    Appl Environ Microbiol 79:6885-93. 2013
    ..Our study demonstrates that no single laboratory assay can predict antifouling effects and that a combination of laboratory and real-life methods must be used to determine the potential antifouling capability of new agents or organisms. ..
  42. pmc Wide distribution of closely related, antibiotic-producing Arthrobacter strains throughout the Arctic Ocean
    Matthias Wietz
    National Food Institute, Technical University of Denmark, Kgs Lyngby, Denmark
    Appl Environ Microbiol 78:2039-42. 2012
    ..Arthrobacter spp. produced arthrobacilins A to C under different culture conditions, but other, unidentified compounds likely contributed to their antibiotic activity...
  43. pmc Antimicrobial peptides effectively kill a broad spectrum of Listeria monocytogenes and Staphylococcus aureus strains independently of origin, sub-type, or virulence factor expression
    Caroline Trebbien Gottlieb
    National Institute of Aquatic Resources, Technical University of Denmark, Søltofts Plads, Bldg, 221, DK 2800 Kgs, Lyngby, Denmark
    BMC Microbiol 8:205. 2008
    ..Hence it is important to determine the natural variation in susceptibility to HDPs to ensure a successful use in clinical treatment regimes...
  44. ncbi request reprint Selection and identification of autochthonous potential probiotic bacteria from turbot larvae (Scophthalmus maximus) rearing units
    Mette Hjelm
    Danish Institute for Fisheries Research, Department of Seafood Research, c o Technical University of Denmark, Kgs Lyngby, Denmark
    Syst Appl Microbiol 27:360-71. 2004
    ..were not lethal to egg yolk sac turbot larvae and in two of three trials, the mortality of larvae decreased (p > 0.001) in treatments where 10(7) cfu/ml Roseobacter sp. strain 27-4 was added, indicating a probiotic potential...
  45. ncbi request reprint Quorum-sensing-directed protein expression in Serratia proteamaculans B5a
    Allan B Christensen
    Section of Molecular Microbiology, BioCentrum DTU, Building 301, Technical University of Denmark, DK 2800, Kgs Lyngby, Denmark
    Microbiology 149:471-83. 2003
    ..This control appeared to be direct and not mediated via LipB. The data presented here demonstrate that quorum-sensing-controlled exoenzymic activities affect food quality...
  46. ncbi request reprint Production of acylated homoserine lactones by different serotypes of Vibrio anguillarum both in culture and during infection of rainbow trout
    Christiane Buch
    Danish Institute for Fisheries Research, Søltofts Plads, c o Technical University of Denmark Bldg 221, Kgs Lyngby, Denmark
    Syst Appl Microbiol 26:338-49. 2003
    ..Our data show that AHLs are produced by almost all V. anguillarum strains and that no clear pattern relating AHL production to disease or virulence appear...
  47. pmc Presence of acylated homoserine lactones (AHLs) and AHL-producing bacteria in meat and potential role of AHL in spoilage of meat
    Jesper Bartholin Bruhn
    Danish Institute for Fisheries Research, Department of Seafood Research, Søltofts Plads, c o Technical University of Denmark Bldg 221, DK 2800 Kgs Lyngby, Denmark
    Appl Environ Microbiol 70:4293-302. 2004
    ..alvei may induce food quality-relevant phenotypes in other bacterial species in the same environment. H. alvei may thus influence spoilage of food products in which Enterobacteriaceae participate in the spoilage process...
  48. ncbi request reprint Quorum sensing signal molecules (acylated homoserine lactones) in gram-negative fish pathogenic bacteria
    Jesper B Bruhn
    Danish Institute for Fisheries Research, Department of Seafood Research, Søltoft Plads c o Technical University of Denmark bldg 221, 2800 Kgs Lyngby, Denmark
    Dis Aquat Organ 65:43-52. 2005
    ....
  49. pmc Gene sequence based clustering assists in dereplication of Pseudoalteromonas luteoviolacea strains with identical inhibitory activity and antibiotic production
    Nikolaj G Vynne
    National Food Institute, Technical University of Denmark, Søltofts Plads Bldg 221, DK 2800 Kgs Lyngby, Denmark
    Mar Drugs 10:1729-40. 2012
    ....
  50. ncbi request reprint High-resolution genotyping of Listeria monocytogenes by fluorescent amplified fragment length polymorphism analysis compared to pulsed-field gel electrophoresis, random amplified polymorphic DNA analysis, ribotyping, and PCR-restriction fragment length po
    Birte Fonnesbech Vogel
    Danish Institute for Fisheries Research, Department of Seafood Research, Søltofts Plads, c o Technical University of Denmark, Building 221, DK 2800 Kgs Lyngby, Denmark
    J Food Prot 67:1656-65. 2004
    ..monocytogenes clones across species. Overall, AFLP fingerprinting was suitable for the high-resolution genotyping of L. monocytogenes and had an equally high or higher differentiation power compared to PFGE or RAPD typing...
  51. pmc Influence of sublethal concentrations of common disinfectants on expression of virulence genes in Listeria monocytogenes
    Vicky G Kastbjerg
    National Institute of Aquatic Resources, Technical University of Denmark, Søltofts Plads Bldg 221, DK 2800 Kongens Lyngby, Denmark
    Appl Environ Microbiol 76:303-9. 2010
    ..Further studies are needed to elucidate whether the changes in virulence gene expression induced by the disinfectants have impact on virulence or other biological properties, such as antibiotic resistance...
  52. pmc Resistance and tolerance to tropodithietic acid, an antimicrobial in aquaculture, is hard to select
    Cisse Hedegaard Porsby
    National Food Institute, Division of Industrial Food Research, Technical University of Denmark, Søltofts Plads, Bldg 221, DK 2800 Kgs Lyngby, Denmark
    Antimicrob Agents Chemother 55:1332-7. 2011
    ..TDA is a promising broad-spectrum antimicrobial in part due to the fact that enhanced tolerance is difficult to gain and that the TDA-tolerant phenotype appears to confer only low-level resistance and is very unstable...
  53. ncbi request reprint The survival of Listeria monocytogenes during long term desiccation is facilitated by sodium chloride and organic material
    Birte Fonnesbech Vogel
    Technical University of Denmark, National Food Institute, Søltofts Plads, Building 221, DK 2800 Kgs Lyngby, Denmark
    Int J Food Microbiol 140:192-200. 2010
    ..Our study shows that the ability to survive for months during desiccated conditions may be a factor explaining the ability of L. monocytogenes to persist in food processing environments...
  54. ncbi request reprint Comparison of sodium hypochlorite-based foam and peroxyacetic acid-based fog sanitizing procedures in a salmon smokehouse: survival of the general microflora and Listeria monocytogenes
    Dorthe Bagge-Ravn
    Danish Institute for Fisheries Research, Department of Seafood Research, Technical University of Denmark, Søltofts Plads, Building 221, DK 2800 Kgs Lyngby, Denmark
    J Food Prot 66:592-8. 2003
    ..monocytogenes was found only in poorly cleaned areas such as drains. The RAPD types for all positive samples were identical to the type that had persisted in the smokehouse since 1995, indicating the importance of drains as a niche...
  55. pmc Response of Listeria monocytogenes to Disinfection Stress at the Single-Cell and Population Levels as Monitored by Intracellular pH measurements and viable-cell counts
    Vicky G Kastbjerg
    National Institute of Aquatic Resources, Technical University of Denmark, Kongens Lyngby, Denmark
    Appl Environ Microbiol 75:4550-6. 2009
    ..Hence a major subpopulation more tolerant to disinfectants, and hence more persistent, does not appear to be present...
  56. pmc Survival of bactericidal antibiotic treatment by a persister subpopulation of Listeria monocytogenes
    Gitte M Knudsen
    Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark
    Appl Environ Microbiol 79:7390-7. 2013
    ..Our study adds L. monocytogenes to the list of bacterial species capable of surviving bactericidal antibiotics in a dormant stage, and this persister phenomenon should be borne in mind when developing treatment regimens. ..
  57. pmc Bactericidal antibiotics do not appear to cause oxidative stress in Listeria monocytogenes
    Louise Feld
    The National Food Institute, Technical University of Denmark, Kongens Lyngby, Denmark
    Appl Environ Microbiol 78:4353-7. 2012
    ..Hence, in this noncyclic metabolism, there is a decoupling between the antibiotic-mediated cellular requirement for NADH and the induction of TCA enzyme activity, which is believed to mediate the oxidative stress reaction...
  58. pmc Marine bacteria from Danish coastal waters show antifouling activity against the marine fouling bacterium Pseudoalteromonas sp. strain S91 and zoospores of the green alga Ulva australis independent of bacteriocidal activity
    Nete Bernbom
    National Food Institute, Technical University of Denmark, Søltofts Plads, Building 221, DK 2800 Kgs Lyngby, Denmark
    Appl Environ Microbiol 77:8557-67. 2011
    ..The antifouling alpP gene was detected only in P. tunicata strains (with purple and yellow pigmentation), so other compounds/mechanisms must be present in the other Pseudoalteromonas strains with antifouling activity...
  59. pmc Bacterial membrane activity of α-peptide/β-peptoid chimeras: influence of amino acid composition and chain length on the activity against different bacterial strains
    Line Hein-Kristensen
    Division of Industrial Food Research, National Food Institute, Technical University of Denmark, Søltofts Plads, Lyngby, DK Denmark
    BMC Microbiol 11:144. 2011
    ..In this report, six α-peptide/β-peptoid chimeras were examined for the effect of amino acid/peptoid substitutions and chain length on the membrane perturbation and subsequent killing of food-borne and clinical bacterial isolates...
  60. pmc Curvularia haloperoxidase: antimicrobial activity and potential application as a surface disinfectant
    Eva H Hansen
    Novozymes A S, DK 2880 Bagsvaerd, Denmark
    Appl Environ Microbiol 69:4611-7. 2003
    ..In conclusion, the Curvularia haloperoxidase system is an effective sanitizing system and has the potential for a vast range of applications, for instance, for disinfection of contact lenses or medical devices...
  61. ncbi request reprint Biofilm formation and antibiotic production in Ruegeria mobilis are influenced by intracellular concentrations of cyclic dimeric guanosinmonophosphate
    Paul W D'Alvise
    Department of Systems Biology, Technical University of Denmark, Søltofts Plads, Bldg 221, DK 2800, Kgs Lyngby, Denmark
    Environ Microbiol 16:1252-66. 2014
    ..In conclusion, c-di-GMP signalling controls biofilm formation and biofilm-associated traits in R. mobilis and, as suggested by presence of GGDEF and EAL domain protein genes, also in other Roseobacter clade species. ..
  62. pmc Sublethal triclosan exposure decreases susceptibility to gentamicin and other aminoglycosides in Listeria monocytogenes
    Ellen G Christensen
    National Food Institute, Technical University of Denmark, Søltofts Plads, Bldg 221, DK 2800 Kgs Lyngby, Denmark
    Antimicrob Agents Chemother 55:4064-71. 2011
    ..The triclosan-induced resistance is, hence, of great concern. Further investigations are needed to determine the molecular mechanisms underlying the effect of triclosan...
  63. doi request reprint Explorative solid-phase extraction (E-SPE) for accelerated microbial natural product discovery, dereplication, and purification
    Maria Mansson
    Center for Microbial Biotechnology, Institute for Systems Biology, Technical University of Denmark, Søltofts Plads, Building 221, DK 2800 Kgs Lyngby, Denmark
    J Nat Prod 73:1126-32. 2010
    ..Overall, this allows fast extract prioritization, easier dereplication, mapping of biological activities, and formulation of a purification strategy...
  64. doi request reprint Chitin stimulates production of the antibiotic andrimid in a Vibrio coralliilyticus strain
    Matthias Wietz
    National Food Institute, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
    Environ Microbiol Rep 3:559-64. 2011
    ..In addition, S2052 appeared less pathogenic towards Artemia than reported for the type strain. Different physiologies of S2052 and closely related strains indicated that V. coralliilyticus subspecies may be adapted to different niches...
  65. pmc Production of bioactive secondary metabolites by marine vibrionaceae
    Maria Mansson
    Center from Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark
    Mar Drugs 9:1440-68. 2011
    ..This account underlines the pending potential for exploring new bacterial sources of bioactive compounds and the challenges related to their investigation...
  66. ncbi request reprint Fermentation and microflora of plaa-som, a thai fermented fish product prepared with different salt concentrations
    Christine Paludan-Müller
    Department of Seafood Research, Danish Institute for Fisheries Research, Technical University of Denmark, Lyngby
    Int J Food Microbiol 73:61-70. 2002
    ..The growth of Z. rouxii has no influence on the fermentation rate, but may contribute positively to the flavour development of the product...
  67. pmc Genome sequencing identifies two nearly unchanged strains of persistent Listeria monocytogenes isolated at two different fish processing plants sampled 6 years apart
    Anne Holch
    National Food Institute, Technical University of Denmark, Kgs Lyngby, Denmark
    Appl Environ Microbiol 79:2944-51. 2013
    ....
  68. pmc Role of acetate in production of an autoinducible class IIa bacteriocin in Carnobacterium piscicola A9b
    Lilian Nilsson
    Danish Institute for Fisheries Research, Department of Seafood Research, c o Technical University of Denmark, DK 2800 Lyngby, Denmark
    Appl Environ Microbiol 68:2251-60. 2002
    ..Based on the results, carnobacteriocin B2 was used as an induction factor to manipulate the production of bacteriocin in cold smoked salmon juice and thus improve the ability to inhibit L. monocytogenes...
  69. ncbi request reprint Antilisterial activity of a Carnobacterium piscicola isolated from Brazilian smoked fish (surubim [Pseudoplatystoma sp.]) and its activity against a persistent strain of Listeria monocytogenes isolated from surubim
    Virgínia F Alves
    Universidade de Sao Paulo, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Av do Café s n Monte Alegre, 14040 903 Ribeirão Preto SP, Brazil
    J Food Prot 68:2068-77. 2005
    ..C. piscicola C2 has the potential for use as a bioprotective culture in surubim and other lightly preserved fish, but further studies are required to optimize its effect...
  70. ncbi request reprint Shewanella hafniensis sp. nov. and Shewanella morhuae sp. nov., isolated from marine fish of the Baltic Sea
    Masataka Satomi
    National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama, 236 8648, Japan
    Int J Syst Evol Microbiol 56:243-9. 2006
    ..Based on the evidence presented, two novel species, Shewanella hafniensis sp. nov. (type strain P010T = ATCC BAA-1207T = NBRC 100975T) and Shewanella morhuae sp. nov. (type strain U1417T = ATCC BAA-1205T = NBRC 100978T), are described...
  71. pmc Elucidation of the Vibrio anguillarum genetic response to the potential fish probiont Pseudomonas fluorescens AH2, using RNA-arbitrarily primed PCR
    Kim Holmstrøm
    Biotechnological Institute, Department of Molecular Characterization, Kogle Alle 2, DK 2970 Hørsholm, Denmark
    J Bacteriol 185:831-42. 2003
    ..anguillarum and complete growth arrest...
  72. pmc Genetic dissection of tropodithietic acid biosynthesis by marine roseobacters
    Haifeng Geng
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, MD 21202, USA
    Appl Environ Microbiol 74:1535-45. 2008
    ..The implications of these results to roseobacter survival and the interaction between TM1040 and its dinoflagellate host are discussed...
  73. ncbi request reprint Description of Shewanella glacialipiscicola sp. nov. and Shewanella algidipiscicola sp. nov., isolated from marine fish of the Danish Baltic Sea, and proposal that Shewanella affinis is a later heterotypic synonym of Shewanella colwelliana
    Masataka Satomi
    National Research Institute of Fisheries Science, Fisheries Research Agency, 2 12 4 Fukuura, Kanazawa Ku, Yokohama 236 8648, Japan
    Int J Syst Evol Microbiol 57:347-52. 2007
    ..nov. (type strain S13T=LMG 23746T=NBRC 102032T) for group 2 strains, and we propose that Shewanella affinis as a later heterotypic synonym of Shewanella colwelliana...
  74. ncbi request reprint Genetic variability among isolates of Listeria monocytogenes from food products, clinical samples and processing environments, estimated by RAPD typing
    Iciar Martinez
    SINTEF Fisheries and Aquaculture Ltd, N 7465 Trondheim, Norway
    Int J Food Microbiol 84:285-97. 2003
    ..monocytogenes must be treated as potentially harmful...
  75. ncbi request reprint Bacteria of the Roseobacter clade show potential for secondary metabolite production
    Torben Martens
    Institute for Chemistry and Biology of the Marine Environment ICBM, University of Oldenburg, PO Box 2503, D 26111 Oldenburg, Germany
    Microb Ecol 54:31-42. 2007
    ..Our screening results therefore suggest that the Roseobacter clade is indeed employing PKS/NRPS biochemistry and should thus be further studied as a potential and largely untapped source of secondary metabolites...
  76. ncbi request reprint Processing of seminal plasma hCAP-18 to ALL-38 by gastricsin: a novel mechanism of generating antimicrobial peptides in vagina
    Ole E Sørensen
    Granulocyte Research Laboratory, The Department of Hematology, Copenhagen University Hospital, Rigshospitalet, DK 2100 Copenhagen, Denmark
    J Biol Chem 278:28540-6. 2003
    ..This enzymatic activation of a proantimicrobial substance in seminal plasma following exposure to the vaginal milieu represents a novel mechanism to prevent infection following sexual intercourse...
  77. pmc Elucidation of the antibacterial mechanism of the Curvularia haloperoxidase system by DNA microarray profiling
    Eva H Hansen
    Novozymes A S, DK 2880 Bagsvaerd, Denmark
    Appl Environ Microbiol 70:1749-57. 2004
    ..coli--namely, the Cpx pathway, which is important for managing the stress response from the Curvularia haloperoxidase system...