J F Rehfeld

Summary

Affiliation: Copenhagen University Hospital
Country: Denmark

Publications

  1. ncbi Naming progastrin-derived peptides
    Jens F Rehfeld
    Department of Clinical Biochemistry KB 3014, Rigshospitalet, University of Copenhagen, DK 2100, Denmark
    Regul Pept 120:177-83. 2004
  2. doi Beginnings: a reflection on the history of gastrointestinal endocrinology
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    Regul Pept 177:S1-5. 2012
  3. doi Pitfalls in diagnostic gastrin measurements
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    Clin Chem 58:831-6. 2012
  4. doi Supersensitive gastrin assay using antibodies raised against a cholecystokinin homolog
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Denmark
    Scand J Clin Lab Invest 72:175-9. 2012
  5. pmc Gastroparesis is associated with oxytocin deficiency, oesophageal dysmotility with hyperCCKemia, and autonomic neuropathy with hypergastrinemia
    Julia Borg
    Department of Clinical Sciences, Gastroenterology Division, Malmo University Hospital, Lund University, Lund, Sweden
    BMC Gastroenterol 9:17. 2009
  6. ncbi Prohormone convertases 1/3 and 2 together orchestrate the site-specific cleavages of progastrin to release gastrin-34 and gastrin-17
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    Biochem J 415:35-43. 2008
  7. ncbi [Diagnosis of neuroendocrine tumours in the gastrointestinal tract]
    Jens F Rehfeld
    Klinisk Biokemisk Afdeling KB3014, Rigshospitalet, 2100 København Ø, Denmark
    Ugeskr Laeger 172:2957-60. 2010
  8. doi Cell-specific precursor processing
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, 2100, Copenhagen, Denmark
    Results Probl Cell Differ 50:45-62. 2010
  9. doi The art of measuring gastrin in plasma: a dwindling diagnostic discipline?
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University Hospital of Copenhagen, Denmark
    Scand J Clin Lab Invest 68:353-61. 2008
  10. pmc The cell-specific pattern of cholecystokinin peptides in endocrine cells versus neurons is governed by the expression of prohormone convertases 1/3, 2, and 5/6
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, Blegdamsvej 9, Copenhagen, Denmark
    Endocrinology 149:1600-8. 2008

Detail Information

Publications116 found, 100 shown here

  1. ncbi Naming progastrin-derived peptides
    Jens F Rehfeld
    Department of Clinical Biochemistry KB 3014, Rigshospitalet, University of Copenhagen, DK 2100, Denmark
    Regul Pept 120:177-83. 2004
    ..Each fragment can be specified with suffixes corresponding to the derived sequence in progastrin...
  2. doi Beginnings: a reflection on the history of gastrointestinal endocrinology
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    Regul Pept 177:S1-5. 2012
    ..From shadowy beginnings, gastrointestinal endocrinology has emerged as a central discipline in the understanding of multicellular life and its diseases...
  3. doi Pitfalls in diagnostic gastrin measurements
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    Clin Chem 58:831-6. 2012
    ..Gastrin circulates as several bioactive peptides, however, and the peptide pattern in gastrinoma patients often deviates from normal. Therefore, it is necessary to measure all forms of gastrin...
  4. doi Supersensitive gastrin assay using antibodies raised against a cholecystokinin homolog
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Denmark
    Scand J Clin Lab Invest 72:175-9. 2012
    ..The results may encourage similar approaches for other peptides using homologue-raised antibodies when supersensitivity is required...
  5. pmc Gastroparesis is associated with oxytocin deficiency, oesophageal dysmotility with hyperCCKemia, and autonomic neuropathy with hypergastrinemia
    Julia Borg
    Department of Clinical Sciences, Gastroenterology Division, Malmo University Hospital, Lund University, Lund, Sweden
    BMC Gastroenterol 9:17. 2009
    ..The aim of this study was to examine if concentrations of oxytocin, cholecystokinin (CCK), gastrin and vasopressin in plasma differ between diabetics with normal function and dysfunction in GI motility...
  6. ncbi Prohormone convertases 1/3 and 2 together orchestrate the site-specific cleavages of progastrin to release gastrin-34 and gastrin-17
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    Biochem J 415:35-43. 2008
    ..Moreover, the results show that PCs react differently to the same dibasic sequences, suggesting that additional structural factors modulate the substrate specificity...
  7. ncbi [Diagnosis of neuroendocrine tumours in the gastrointestinal tract]
    Jens F Rehfeld
    Klinisk Biokemisk Afdeling KB3014, Rigshospitalet, 2100 København Ø, Denmark
    Ugeskr Laeger 172:2957-60. 2010
    ..The infrequency and demand for analytical know-how make the case for centralization of diagnostic examinations...
  8. doi Cell-specific precursor processing
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, 2100, Copenhagen, Denmark
    Results Probl Cell Differ 50:45-62. 2010
    ..Notably, sick cells - not least the neoplastic cells - often process prohormones in a manner different from that of the normal endocrine cells...
  9. doi The art of measuring gastrin in plasma: a dwindling diagnostic discipline?
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University Hospital of Copenhagen, Denmark
    Scand J Clin Lab Invest 68:353-61. 2008
    ..This review discusses the art of measuring gastrin in plasma with adequate diagnostic specificity...
  10. pmc The cell-specific pattern of cholecystokinin peptides in endocrine cells versus neurons is governed by the expression of prohormone convertases 1/3, 2, and 5/6
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, Blegdamsvej 9, Copenhagen, Denmark
    Endocrinology 149:1600-8. 2008
    ..The results suggest that the different peptide patterns in the brain and the gut are due to different expression of PCs...
  11. doi The Zollinger-Ellison syndrome and mismeasurement of gastrin
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    Gastroenterology 140:1444-53. 2011
    ..Zollinger-Ellison syndrome (ZES) is characterized by hypersecretion of gastric acid, severe peptic ulcerations in the upper small intestine, and diarrhea. It is usually diagnosed by measuring increased levels of gastrin in plasma...
  12. ncbi [Poul Astrup--blood gases, acids and bases]
    Jens F Rehfeld
    Klinisk Biokemisk Afdeling, Rigshospitalet, DK 2100 København
    Ugeskr Laeger 169:2896. 2007
  13. doi Unsulfated cholecystokinin: An overlooked hormone?
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Denmark
    Regul Pept 173:1-5. 2012
    ..This review discusses the occurrence, molecular forms, and possible physiological as well as pathophysiological significance of unsulfated CCK peptides...
  14. ncbi The biology of cholecystokinin and gastrin peptides
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    Curr Top Med Chem 7:1154-65. 2007
    ..A carcinogenetic significance of gastrin peptides remains, however, to be proven...
  15. doi Incretin physiology beyond glucagon-like peptide 1 and glucose-dependent insulinotropic polypeptide: cholecystokinin and gastrin peptides
    J F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    Acta Physiol (Oxf) 201:405-11. 2011
    ..Therefore, a short review of the incretin system in a broader functional context that includes gastrin and CCK peptides may be timely...
  16. ncbi The expression of peptide hormones in normal cells and tumour cells
    J F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Denmark
    Acta Oncol 30:429-33. 1991
    ..Awareness of such cell-specific processing patterns is important for the understanding of ectopic synthesis in neuroendocrine tumours...
  17. ncbi Gut hormones, early dumping and resting energy expenditure in patients with good and poor weight loss response after Roux-en-Y gastric bypass
    C Dirksen
    1 Department of Endocrinology, Hvidovre Hospital, University of Copenhagen, Hvidovre, Denmark 2 Novo Nordisk Foundation Centre for Basic Metabolic Research, The Panum Institute, University of Copenhagen, Copenhagen N, Denmark
    Int J Obes (Lond) 37:1452-9. 2013
    ..To identify factors contributing to the variation in weight loss after Roux-en-Y gastric bypass (RYGB)...
  18. ncbi On the tissue-specific processing of procholecystokinin in the brain and gut--a short review
    J F Rehfeld
    University Department of Clinical Biochemistry Rigshospitalet, Copenhagen, Denmark
    J Physiol Pharmacol 54:73-9. 2003
    ....
  19. ncbi Accurate measurement of cholecystokinin in plasma
    J F Rehfeld
    Dept of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark
    Clin Chem 44:991-1001. 1998
    ..Chromatography of human plasma revealed traces of CCK-58, a predominance of CCK-33 and CCK-22, and moderate amounts of CCK-8. The results show that it is possible to produce specific CCK-antisera using a sulfated CCK-12 analog...
  20. ncbi A processing enzyme cleaving avian progastrin at post-Phe bonds
    H Jensen
    Department of Clinical Biochemistry, Rigshospitalet (National University Hospital, Copenhagen, Denmark
    Biochim Biophys Acta 1547:64-71. 2001
    ..Taken together, the results support the existence of vertebrate endoprotease cleaving hormone precursors at post-Phe sites...
  21. ncbi Unique progastrin processing in equine G-cells suggests marginal tyrosyl sulfotransferase activity
    A H Johnsen
    Department of Clinical Biochemistry, Rigshospitalet, The National University Hospital, Copenhagen, Denmark
    Eur J Biochem 255:432-8. 1998
    ..Furthermore, the marginal sulphation may explain the high proportion of gastrin-34 versus gastrin-17 in the equine antrum, since tyrosyl sulphation has been shown to promote the endoproteolytic processing of prohormones...
  22. ncbi cDNA deduced procionin. Structure and expression in protochordates resemble that of procholecystokinin in mammals
    H J Monstein
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Denmark
    FEBS Lett 331:60-4. 1993
    ..Hence, the tissue-specific expression of the cionin gene in Ciona intestinalis resembles that of the CCK gene in mammals...
  23. ncbi Acute myocardial hypoxia increases BNP gene expression
    J P Goetze
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    FASEB J 18:1928-30. 2004
    ..Accordingly, the plasma proBNP concentration increased after 2 h of myocardial hypoxia (P=0.028). Cumulatively, the data suggest that acute hypoxia stimulates cardiac BNP expression...
  24. ncbi Changes in gastrointestinal hormone responses, insulin sensitivity, and beta-cell function within 2 weeks after gastric bypass in non-diabetic subjects
    S H Jacobsen
    Department of Endocrinology 541, Hvidovre Hospital, University of Copenhagen, Kettegaard Alle 30, 2650 Hvidovre, Copenhagen, Denmark
    Obes Surg 22:1084-96. 2012
    ..We present a detailed analysis of the early hormone changes after RYGB in response to three different oral test meals designed to provide this information without causing side effects (such as dumping)...
  25. ncbi Gallbladder emptying and cholecystokinin response to fish oil and trioleate ingestion
    C Riber
    Department of Surgical Gastroenterology C, University of Copenhagen, Denmark
    Digestion 57:161-4. 1996
    ..Gastric emptying as well as the release of gastrin and secretin were similar after the two types of fat. The results suggest that the reduced gallbladder emptying after fish oil may be due to a smaller release of CCK...
  26. ncbi Identification of gastrin component I as gastrin-71. The largest possible bioactive progastrin product
    J F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Denmark
    Eur J Biochem 223:765-73. 1994
    ..The results also suggest that progastrin may follow two different processing pathways of which the less trafficked releases gastrin-71...
  27. ncbi New consensus features for tyrosine O-sulfation determined by mutational analysis
    J R Bundgaard
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, DK 2100 Copenhagen, Denmark
    J Biol Chem 272:21700-5. 1997
    ..Our data suggest a considerably broader range of substrates for the tyrosylprotein sulfotransferase than hitherto assumed and that the tyrosylprotein sulfotransferase is cell-specifically expressed...
  28. pmc Elasmobranchs express separate cholecystokinin and gastrin genes
    A H Johnsen
    Department of Clinical Biochemistry, Rigshospitalet National University Hospital, Copenhagen, Denmark
    Proc Natl Acad Sci U S A 94:10221-6. 1997
    ..This event must have occurred before, or perhaps during, the evolution of cartilagenous fishes, probably concomitant with the occurrence of gastric acid secretion...
  29. pmc Post-poly(Glu) cleavage and degradation modified by O-sulfated tyrosine: a novel post-translational processing mechanism
    J F Rehfeld
    Department of Clinical Biochemistry, University of Copenhagen, Denmark
    EMBO J 14:389-96. 1995
    ..Moreover, gastrin-7 is apparently trimmed, with subsequent accumulation of sulfated gastrin-6. Consequently, O-sulfated tyrosine ensures production of a new hormone which stimulates gastric acid secretion as potently as gastrin-17...
  30. ncbi A centenary of gastrointestinal endocrinology
    J F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, 2100 Copenhagen, Denmark
    Horm Metab Res 36:735-41. 2004
    ....
  31. ncbi How to measure cholecystokinin in tissue, plasma and cerebrospinal fluid
    J F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Denmark
    Regul Pept 78:31-9. 1998
    ..These antibodies have to be exquisitely specific for the 0-sulfated C-terminal heptapeptide amide of CCK without binding the similar gastrin epitope. Only few of such antibodies have been raised...
  32. ncbi The first PP supper--Camelot at Bispebjerg
    T W Schwartz
    Department of Pharmacology, University of Copenhagen, Denmark
    Scand J Clin Lab Invest Suppl 234:109-21. 2001
    ..The period ended in the summer of 1976, when Rehfeld moved his court to the University of Aarhus and the others scattered--each to establish their own scientific group...
  33. ncbi Peptide hormone expression and precursor processing
    J F Rehfeld
    Department of Clinical Chemistry, Rigshospitalet, University of Copenhagen, Denmark
    Acta Oncol 28:315-8. 1989
    ..Awareness of such cell-specific processing patterns is important for the understanding of ectopic synthesis in neuroendocrine tumours...
  34. ncbi Heterologous expression of human cholecystokinin in Saccharomyces cerevisiae. Evidence for a lysine-specific endopeptidase in the yeast secretory pathway
    I J Rourke
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen O, Denmark
    J Biol Chem 272:9720-7. 1997
    ..Our data suggests that an as yet uncharacterized endopeptidase(s) in the S. cerevisiae secretory pathway is responsible for the lysine-specific cleavage of pro-CCK...
  35. ncbi An intron 1 polymorphism in the cholecystokinin-A receptor gene associated with schizophrenia in males
    P Koefoed
    Department of Neuroscience and Pharmacology, Laboratory for Neuropsychiatry, University of Copenhagen and Centre of Psychiatry, Rigshospitalet, Denmark
    Acta Psychiatr Scand 120:281-7. 2009
    ..To identify whether a genetic variation (rs1800857; IVS1-5T>C) in the neuropeptide cholecystokinin-A receptor (CCKAR) gene is a risk factor in the pathogenesis of schizophrenia...
  36. ncbi Mitogen-activated protein kinase and protein kinase A signaling pathways stimulate cholecystokinin transcription via activation of cyclic adenosine 3',5'-monophosphate response element-binding protein
    T V Hansen
    Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark
    Mol Endocrinol 13:466-75. 1999
    ..We propose that bFGF in combination with neurotransmitters/neuropeptides coupling to the PKA-signaling pathway play an important role in the control of CCK gene expression...
  37. ncbi Composite action of three GC/GT boxes in the proximal promoter region is important for gastrin gene transcription
    T O Hansen
    Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark
    Mol Cell Endocrinol 155:1-8. 1999
    ..Both effects were dependent on the C-terminal DNA binding domain of Sp3. The results indicates that the combined effect of the GC/GT boxes and the ratio between Sp1 and Sp3 are important for gastrin gene expression...
  38. ncbi Inhibition of human gastric lipase by intraduodenal fat involves glucagon-like peptide-1 and cholecystokinin
    M Wøjdemann
    Department of Surgery, Rigshospitalet, National University Hospital, Copenhagen, Denmark
    Regul Pept 80:101-6. 1999
    ..5 to 15.1 +/- 1.5 pmol/l (P < 0.05) and from 1.2 +/- 0.4 to 3.8 +/- 0.9 pmol/l (P < 0.05). This study reveals a negative effect of fat in the duodenum on gastric lipase secretion. This effect may be mediated by GLP-1 and/or CCK...
  39. ncbi The posttranslational phase of gene expression: new possibilities in molecular diagnosis
    J F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    Curr Mol Med 3:25-38. 2003
    ..But first general aspects of the posttranslational processing are reviewed as a necessary basis for the understanding of the new diagnostic possibilities...
  40. ncbi Increased synthesis but decreased processing of neuronal proCCK in prohormone convertase 2 and 7B2 knockout animals
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Denmark
    J Neurochem 83:1329-37. 2002
    ..The results show that PC2 plays a major neuron-specific role in the processing of proCCK...
  41. doi Posttranslational processing of progastrin
    Jens R Bundgaard
    Department of Clinical Biochemistry, KB 3014, Rigshospitalet, University of Copenhagen, Blegdamsvej 9, 2100, Copenhagen, Denmark
    Results Probl Cell Differ 50:207-20. 2010
    ..Thus, the review describes how the processing depends on the cell-specific expression of the processing enzymes and kinetics in the secretory pathway...
  42. ncbi Progastrin processing differs in 7B2 and PC2 knockout animals: a role for 7B2 independent of action on PC2
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    FEBS Lett 510:89-93. 2002
    ..The results show that PC2 and 7B2 both are required for synthesis of the main form of gastrin (gastrin-17), and that 7B2 exhibits effects beyond PC2-mediated cleavages...
  43. ncbi Increased cardiac BNP expression associated with myocardial ischemia
    J P Goetze
    Department of Clinical Biochemistry, KB 3011, Rigshospitalet, University of Copenhagen, 9 Blegdamsvej, DK 2100 Copenhagen, Denmark
    FASEB J 17:1105-7. 2003
    ..Thus, elevated BNP and proBNP concentrations do not necessarily reflect heart failure but may also result from cardiac ischemia...
  44. ncbi Reduced ghrelin, islet amyloid polypeptide, and peptide YY expression in the stomach of gastrin-cholecystokinin knockout mice
    Lennart Friis-Hansen
    Department of Clinical Biochemistry, KB 3011, Rigshospitalet, 9 Blegdamsvej, DK 2100 Copenhagen, Denmark
    Endocrinology 146:4464-71. 2005
    ..Both gastrin and combined gastrin-CCK deficiency reduced the gastric IAPP and PYY expression...
  45. ncbi The predominant cholecystokinin in human plasma and intestine is cholecystokinin-33
    J F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, 2100 Copenhagen, Denmark
    J Clin Endocrinol Metab 86:251-8. 2001
    ..Its predominance in feline intestines, however, was confirmed. Hence, the results show a significant species variation and emphasize the necessity of highly specific and well characterized assays in molecular studies of CCK...
  46. ncbi Identification of four chicken gastrins, obtained by processing at post-Phe bonds
    I Bjørnskov
    Department of Clinical Biochemistry, University Hospital, Copenhagen, Denmark
    Peptides 13:595-601. 1992
    ..The three shorter forms were all derived from the precursor by post-Phe cleavages. This cleavage pattern suggests a processing enzyme specific for bonds between Phe and moderately hydrophobic residues...
  47. ncbi BNP and N-terminal proBNP are both extracted in the normal kidney
    J P Goetze
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    Eur J Clin Invest 36:8-15. 2006
    ..Information on the regional elimination of the peptides is, however, still scarce. We therefore examined the renal and peripheral extraction of N-terminal proBNP and BNP...
  48. ncbi No association between the -399 C > T polymorphism of the neuropeptide Y gene and schizophrenia, unipolar depression or panic disorder in a Danish population
    C Lindberg
    Laboratory of Neuropsychiatry, Department of Pharmacology, University of Copenhagen and Rigshospitalet University Hospital, Copenhagen, Denmark
    Acta Psychiatr Scand 113:54-8. 2006
    ..The objective of this study was to investigate potential associations between the polymorphism and three psychiatric disorders in a Danish population...
  49. ncbi Cholecystokinin and panic disorder--three unsettled questions
    J F Rehfeld
    Department of Clinical Biochemistry KB 3011, University of Copenhagen, Rigshospitalet, DK 2100, Copenhagen, Denmark
    Regul Pept 93:79-83. 2000
    ....
  50. ncbi Impaired feedback of gastric functions in carboxypeptidase E-deficient mice
    L Friis-Hansen
    Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, DK 2100, Denmark
    Biochem Biophys Res Commun 267:638-42. 2000
    ..Furthermore, the synthesis of antral somatostatin and fundic chromogranin A is also abnormal. Hence the results suggest a breakdown in the feedback loop that regulates gastric acid secretion...
  51. ncbi Cionin: a disulfotyrosyl hybrid of cholecystokinin and gastrin from the neural ganglion of the protochordate Ciona intestinalis
    A H Johnsen
    Department of Clinical Chemistry, Rigshospitalet, University of Copenhagen, Denmark
    J Biol Chem 265:3054-8. 1990
    ..We conclude that the structure of the peptide, named cionin, suits that of a common ancestor for cholecystokinin and gastrin...
  52. ncbi Allelic loss of chromosome 2p21-16.3 is associated with reduced survival in sporadic colorectal cancer
    M L Bisgaard
    Dept. of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark
    Scand J Gastroenterol 36:405-9. 2001
    ..3 was, moreover, associated with increased loss of the other tumour suppressor loci (P = 0.012). CONCLUSIONS: The results show that loss of 2p21-16.3 is an independent indicator of survival in patients with colorectal cancer...
  53. ncbi Antral G-cell in gastrin and gastrin-cholecystokinin knockout animals
    Lennart Friis-Hansen
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    Cell Tissue Res 321:141-6. 2005
    ....
  54. ncbi Progastrin is directed to the regulated secretory pathway by synergistically acting basic and acidic motifs
    Jens R Bundgaard
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, DK 2100 Copenhagen, Denmark
    J Biol Chem 279:5488-93. 2004
    ..This implies that the mutants are constitutively secreted. Our data suggest that the dibasic processing sites constitute the most important sorting domain of progastrin, and these sites act in synergy with the acidic domain...
  55. ncbi The endoproteolytic maturation of progastrin and procholecystokinin
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    J Mol Med (Berl) 84:544-50. 2006
    ..This review emphasizes that prohormone convertases play a decisive but substrate and cell-specific role in the biosynthetic maturation of gastrin and CCK...
  56. ncbi KCl and forskolin synergistically up-regulate cholecystokinin gene expression via coordinate activation of CREB and the co-activator CBP
    Thomas V O Hansen
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    J Neurochem 89:15-23. 2004
    ....
  57. ncbi Increased circulating endothelins are not of cardiopulmonary origin in heart failure patients
    Y Z Wang
    Medical Department B, Cardiac Catheterization Laboratory, The Heart Centre, and Department of Clinical Biochemistry, Copenhagen University Hospital, Rigshospitalet, Denmark
    Scand J Clin Lab Invest 65:341-7. 2005
    ..The aim of this study was to investigate whether the cardiopulmonary system contributes to the circulating endothelin-1 and big-endothelin concentrations in heart failure patients...
  58. ncbi Cholecystokinin-2 receptor mediated gene expression in neuronal PC12 cells
    Thomas V O Hansen
    Department of Clinical Biochemistry, University Hospital Rigshospitalet, Copenhagen, Denmark
    J Neurochem 104:1450-65. 2008
    ..Taken together, we propose that neuronal CCK may have a role in the regulation of the circadian rhythm, the metabolism of cerebral cholesterol and in the regulation of the plasminogen system...
  59. ncbi Antral content, secretion and peripheral metabolism of N-terminal progastrin fragments
    Jens Peter Goetze
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, DK 2100 Copenhagen, Denmark
    Regul Pept 133:47-53. 2006
    ..In order to examine the cellular content, secretion and peripheral metabolism of these fragments, we developed an immunoassay specific for the N-terminal sequence of human progastrin...
  60. ncbi Gastric inflammation, metaplasia, and tumor development in gastrin-deficient mice
    Lennart Friis-Hansen
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    Gastroenterology 131:246-58. 2006
    ..We also explored the associated changes in the gastric microflora and the long-term consequences of gastrin-deficient achlorhydria...
  61. ncbi GSK-3beta reduces cAMP-induced cholecystokinin gene expression by inhibiting CREB binding
    Thomas V O Hansen
    Department of Clinical Biochemistry, University Hospital Rigshospitalet, Blegdamsvej 9, DK 2100 Copenhagen, Denmark
    Neuroreport 15:841-5. 2004
    ..We conclude that GSK-3beta regulates cAMP-induced CCK transcription by reducing CREB binding to the CRE(-80) element in the CCK promoter...
  62. ncbi Distinct linkage between post-translational processing and differential secretion of progastrin derivatives in endocrine cells
    Jens R Bundgaard
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, KB 3014, Copenhagen University Hospital, Blegdamsvej 9, Copenhagen DK 2100, Denmark
    J Biol Chem 283:4014-21. 2008
    ..The results suggest a two-step sorting mechanism for progastrin leading to differential secretion of processed fragments via different secretory pathways...
  63. ncbi Cloning and sequencing of the bovine gastrin gene
    T Lund
    University Department of Clinical Chemistry, Rigshospitalet, Copenhagen, Denmark
    Mol Endocrinol 3:1585-8. 1989
    ..Comparison with human, porcine, and rat cDNA sequences revealed extensive homology in the coding region as well as in short noncoding structures...
  64. ncbi Increased intensity of a single exercise bout stimulates subsequent fat intake
    B Klausen
    Research Department of Human Nutrition, The Royal Veterinary and Agricultural University, Frederiksberg C, Copenhagen, Denmark
    Int J Obes Relat Metab Disord 23:1282-7. 1999
    ..How this change in lifestyle affects the selection of dietary intakes is presently unknown...
  65. pmc Reduced frequency of extracolonic cancers in hereditary nonpolyposis colorectal cancer families with monoallelic hMLH1 expression
    A C Jäger
    Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark
    Am J Hum Genet 61:129-38. 1997
    ..We propose that the mutation results in a milder phenotype, because the mutated hMLH1 protein is prevented from exerting a dominant negative effect on the concerted action of the mismatch repair system...
  66. ncbi Impact of assay epitope specificity in gastrinoma diagnosis
    Jens Peter Goetze
    Department of Clinical Biochemistry, KB 3014, Rigshospitalet, University of Copenhagen, 9 Blegdamsvej, DK 2100 Copenhagen, Denmark
    Clin Chem 49:333-4. 2003
  67. ncbi Chamber-dependent expression of brain natriuretic peptide and its mRNA in normal and diabetic pig heart
    Christina Christoffersen
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    Hypertension 40:54-60. 2002
    ..The data suggest that BNP mRNA processing and proBNP storage differ between the atrial and ventricular myocardium. The results also imply that diabetes increases cardiac BNP expression in a chamber-dependent manner...
  68. ncbi Gastrin-17 specific antibodies are too specific for gastrinoma diagnosis but stain G-cells
    Jens Peter Goetze
    Department of Clinical Biochemistry, University of Copenhagen, Rigshospitalet, Copenhagen, Denmark
    Scand J Gastroenterol 40:596-8. 2005
  69. ncbi Processing-independent quantitation of chromogranin a in plasma from patients with neuroendocrine tumors and small-cell lung carcinomas
    Tine Børglum
    University Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark
    Clin Chem 53:438-46. 2007
    ..To cope with this variability and improve tumor diagnosis, we developed a processing-independent analysis (PIA) method to measure the total CgA product...
  70. doi Processing-independent analysis for pro-C-type natriuretic peptide
    Solvej K Lippert
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    J Immunol Methods 362:32-7. 2010
    ..Taken together, our new proCNP assay shows that proCNP is abundantly present in human seminal plasma and that seminal proCNP is secreted from the prostate gland and/or the seminal vesicles...
  71. ncbi Processing of chicken progastrin at post-Phe bonds by an aspartyl protease
    Hanne Jensen
    Department of Clinical Biochemistry, KB 3013, Rigshospitalet, University of Copenhagen, Blegdamsvej 9, DK 2100 Copenhagen O, Denmark
    Biochim Biophys Acta 1748:43-9. 2005
    ..Experiments using recombinant cathepsin D and E indicated that neither is responsible for the endoproteolytic cleavage of chicken progastrin at post-Phe bonds...
  72. doi C-type natriuretic peptide in prostate cancer
    Soeren Junge Nielsen
    Department of Clinical Biochemistry, Ringhospitalet, University of Copenhagen, Denmark
    APMIS 117:60-7. 2009
    ..2) vs. 15.1 pmol/l (10.4-23.2), p=0.002] but not enough to justify the use of N-terminal proCNP as a cancer marker. Further research is needed to establish whether measurement of proCNP-derived peptides may offer clinical information...
  73. ncbi Impact of epitope specificity and precursor maturation in pro-B-type natriuretic peptide measurement
    Jens Peter Goetze
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Denmark
    Clin Chem 54:1780-7. 2008
    ..In this study, we examined the impact of epitope specificity and precursor maturation on plasma measurement of proBNP-derived peptides...
  74. ncbi Discriminating between cardiac and pulmonary dysfunction in the general population with dyspnea by plasma pro-B-type natriuretic peptide
    Rasmus Mogelvang
    Copenhagen City Heart Study, Epidemiological Research Unit, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark
    J Am Coll Cardiol 50:1694-701. 2007
    ..This study was designed to determine whether measurement of plasma pro-B-type natriuretic peptide (proBNP) could be used in discriminating between cardiac and pulmonary dyspnea in the general population...
  75. ncbi Discordant expression of pro-B-type and pro-C-type natriuretic peptide in newborn infants of mothers with type 1 diabetes
    Mads Nybo
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, 9 Blegdamsvej, DK 2100 Copenhagen, Denmark
    Regul Pept 141:135-9. 2007
    ..As signaling via the C-type natriuretic peptide (CNP) specific receptor protects against cardiac hypertrophy, we examined whether maternal type 1 diabetes affects the plasma concentrations of proCNP-derived peptides in newborn infants...
  76. ncbi Pro-brain natriuretic peptide as marker of cardiovascular or pulmonary causes of dyspnea in patients with terminal parenchymal lung disease
    Jens Peter Goetze
    Medical Department B, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    J Heart Lung Transplant 23:80-7. 2004
    ..Therefore, we measured the plasma concentrations of natriuretic precursor peptides in patients with terminal parenchymal lung disease who had normal left ventricular function and who were referred for evaluation for lung transplantation...
  77. ncbi Impact of hemoglobin on plasma pro-B-type natriuretic peptide concentrations in the general population
    Mads Nybo
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    Clin Chem 53:1921-7. 2007
    ..Recent studies indicate that anemia may also affect proBNP concentrations in patients with heart failure or stroke. However, the impact of hemoglobin status on proBNP concentrations has not been established in the general population...
  78. ncbi The effect of salatrim, a low-calorie modified triacylglycerol, on appetite and energy intake
    Lone B Sørensen
    Department of Human Nutrition, Centre for Advanced Food Studies, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark
    Am J Clin Nutr 87:1163-9. 2008
    ....
  79. ncbi ProCNP and CNP are expressed primarily in male genital organs
    Søren J Nielsen
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Denmark
    Regul Pept 146:204-12. 2008
    ..In conclusion most proCNP-derived peptides are synthesized in epithelial cells in the epididymis, the prostate gland and in the seminal vesicles. The expression in male genital organs suggests a role of CNP in reproduction...
  80. ncbi Characteristics of gastrin controlled ECL cell specific gene expression
    Lennart Friis-Hansen
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Denmark
    Regul Pept 140:153-61. 2007
    ..Knowledge of the molecular biology of the ECL cell is therefore important for understanding gastric physiology. Accordingly, we wanted to identify genes that are characteristically expressed in the ECL cells and controlled by gastrin...
  81. ncbi Enhanced peptide secretion by gene disruption of CYM1, a novel protease in Saccharomyces cerevisiae
    Lars Jønson
    Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark
    Eur J Biochem 271:4788-97. 2004
    ..The protease does not require post-translational processing prior to activation and it contains cytosolic activity that processes peptides designated for the secretory pathway prior to translocation into the endoplasmic reticulum...
  82. ncbi B-type natriuretic peptide and its precursor in cardiac venous blood from failing hearts
    Jens Peter Goetze
    Cardiac Catheterization Laboratory, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    Eur J Heart Fail 7:69-74. 2005
    ..Plasma concentrations of B-type natriuretic peptide (BNP-32) and its precursor (proBNP) are increased in chronic heart failure. Accordingly, BNP-32 and proBNP are both being implemented as clinical markers...
  83. ncbi Measurement of pro-C-type natriuretic peptide in plasma
    Soren Junge Nielsen
    Department of Clinical Biochemistry, Cardiac Catheterization Laboratory, Rigshospitalet, University of Copenhagen, Denmark
    Clin Chem 51:2173-6. 2005
  84. ncbi [Clinical biochemistry: heart hormones. The Danish Society of Biochemistry]
    Jens Peter Gøtze
    Klinisk Biokemisk Afdeling, afsnit 3014, H S Rigshospitalet, DK 2100 København
    Ugeskr Laeger 166:1109. 2004
  85. doi Anatomical characterization of cytoglobin and neuroglobin mRNA and protein expression in the mouse brain
    Christian Ansgar Hundahl
    Department of Neuroscience and Pharmacology, The Panum Institute, University of Copenhagen, Copenhagen, Denmark
    Brain Res 1331:58-73. 2010
    ..We believe other functions than pure oxygen buffers and neuroprotectants should be considered. The anatomical data indicate a role in NO signalling for Cygb and involvement in sleep-wake cycling for Cygb and Ngb...
  86. ncbi Characteristics of the Danish families with multiple endocrine neoplasia type 1
    Anne Charlotte Jäger
    Department of Clinical Biochemistry, Rigshospitalet, Blegdamsvej 9, DK 2100 Copenhagen, Denmark
    Mol Cell Endocrinol 249:123-32. 2006
    ..In contrast, sporadic carcinoid tumours or primary prolactinomas are rarely associated with germ-line MEN1 mutations...
  87. ncbi Gene variations in the cholecystokinin system in patients with panic disorder
    Pernille Koefoed
    Laboratory for Neuropsychiatry, Department of Neuroscience and Pharmacology, University of Copenhagen, Denmark
    Psychiatr Genet 20:59-64. 2010
    ..The neuropeptide cholecystokinin (CCK) and its receptors (the CCK system) have been suggested to be involved in the pathogenesis of PD...
  88. ncbi Quantification of pro-B-type natriuretic peptide and its products in human plasma by use of an analysis independent of precursor processing
    Jens Peter Goetze
    Departments of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, DK 2100 Copenhagen, Denmark
    Clin Chem 48:1035-42. 2002
    ..However, the currently available assays to measure pro-B-type natriuretic peptide (proBNP)-derived peptides have produced grossly discrepant results...
  89. ncbi Plasma pro-B-type natriuretic peptide in the general population: screening for left ventricular hypertrophy and systolic dysfunction
    Jens Peter Goetze
    The Copenhagen City Heart Study, Epidemiological Research Unit, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark
    Eur Heart J 27:3004-10. 2006
    ..We therefore examined whether proBNP measurement is useful for LVH and LVSD screening in the general population...
  90. ncbi Clinical endocrinology and metabolism. Cholecystokinin
    Jens F Rehfeld
    Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Blegdamsvej 9, DK 2100 Copenhagen, Denmark
    Best Pract Res Clin Endocrinol Metab 18:569-86. 2004
    ..Stimulation with exogenous CCK has proved useful in diagnostic tests of gallbladder and pancreatic diseases, as well as medullary thyroid carcinomas...
  91. ncbi Differentiation of gastric ECL cells is altered in CCK(2) receptor-deficient mice
    Duan Chen
    Department of Intra abdominal Diseases and Laboratory Medicine, Norwegian University of Science and Technology, Trondheim, Norway
    Gastroenterology 123:577-85. 2002
    ..This report describes the effects of targeted disruption of the CCK(2) receptor gene on ECL cell morphology and function...
  92. ncbi Prohormonal junk fragments as hormones?
    Jens F Rehfeld
    Gastroenterology 131:1638-40. 2006
  93. ncbi Altered control of gastric acid secretion in gastrin-cholecystokinin double mutant mice
    Duan Chen
    Department of Cancer Research and Molecular Medicine, Norweigian University of Science and Technology, Trondheim, Norway
    Gastroenterology 126:476-87. 2004
    ..Mice lacking gastrin or both gastrin and CCK were examined to determine the role of the hormones...
  94. ncbi Procholecystokinin as marker of human Ewing sarcomas
    Jean Claude Reubi
    Division of Cell Biology and Experimental Cancer Research, Institute of Pathology, University of Berne, Switzerland
    Clin Cancer Res 10:5523-30. 2004
    ....
  95. ncbi Jejunoileal bypass changes the duodenal cholecystokinin and somatostatin cell density
    Leif Ockander
    Department of Internal Medicine, Ryhov s Hospital, Jonkoping, Sweden
    Obes Surg 13:584-90. 2003
    ..Changes in the neuroendocrine system may be affected by the JIB-operation, because the proximal small intestinal mucosa has a rich supply of endocrine cells and peptidergic nerves...
  96. ncbi Influence of COX-2 inhibition by rofecoxib on serum and tumor progastrin and gastrin levels and expression of PPARgamma and apoptosis-related proteins in gastric cancer patients
    Peter C Konturek
    Department of Medicine, University Erlangen Nuremberg, Erlangen, Germany
    Dig Dis Sci 48:2005-17. 2003
    ....
  97. ncbi Gastrin vaccination against gastrointestinal and pancreatic cancer
    Jens F Rehfeld
    Scand J Gastroenterol 41:122-3. 2006
  98. ncbi Long-term morpho-functional development of Helicobacter pylori-induced gastritis in Mongolian gerbils
    Yi Qian Sun
    Department of Biomedicine and Surgery, University of Linkoping, and Pathology Research Department, Ryhov Hospital, Fonköping, Sweden
    Scand J Gastroenterol 40:1157-67. 2005
    ..The aim of this study was to investigate the long-term development of H. pylori-induced gastritis in Mongolian gerbils in terms of morphology, gastrin secretion, epithelial proliferation and gene expression of pro-inflammatory cytokines...
  99. ncbi [Gallstones as symptoms of cholecystokinin resistance]
    Jens F Rehfeld
    Klinisk Biokemisk Afdeling, H S Rigshospitalet, 2100 København Ø
    Ugeskr Laeger 167:2658-9. 2005
  100. ncbi Small gallstones, preserved gallbladder motility, and fast crystallization are associated with pancreatitis
    Niels G Venneman
    Gastrointestinal Research Unit, Departments of Gastroenterology and Surgery, University Medical Center, 3508 GA Utrecht, The Netherlands
    Hepatology 41:738-46. 2005
    ..In conclusion, patients with small gallbladder stones and/or preserved gallbladder motility are at increased risk of pancreatitis. The potential benefit of prophylactic cholecystectomy in this patient category has yet to be explored...
  101. ncbi Serum progastrin and its products, gastric acid secretion and serum pepsinogen I in gastric cancer
    Stanislaw J Konturek
    Department of Physiology, University College of Medicine, Cracow, Poland
    Digestion 68:169-77. 2003
    ..Numerous studies have shown an association between Helicobacter pylori (Hp) infection and gastric cancer (GC)...