Niels Borregaard

Summary

Affiliation: Copenhagen University Hospital
Country: Denmark

Publications

  1. doi What doesn't kill you makes you stronger: the anti-inflammatory effect of neutrophil respiratory burst
    Niels Borregaard
    Department of Hematology, National University Hospital, University of Copenhagen, 2100 Copenhagen, Denmark Electronic address
    Immunity 40:1-2. 2014
  2. doi Proteome profiling of human neutrophil granule subsets, secretory vesicles, and cell membrane: correlation with transcriptome profiling of neutrophil precursors
    Sara Rørvig
    2 Department of Hematology, University of Copenhagen, Rigshospitalet 9322, 20 Juliane Mariesvej, 2100 Copenhagen, Denmark
    J Leukoc Biol 94:711-21. 2013
  3. ncbi Neutrophils and keratinocytes in innate immunity--cooperative actions to provide antimicrobial defense at the right time and place
    Niels Borregaard
    Department of Hematology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    J Leukoc Biol 77:439-43. 2005
  4. ncbi Systems biology of neutrophil differentiation and immune response
    Kim Theilgaard-Monch
    The Granulocyte Research Laboratory, Department of Hematology 9322, Rigshospitalet, University of Copenhagen, Juliane Mariesvej 20, 2100 Copenhagen Ø, Denmark
    Curr Opin Immunol 18:54-60. 2006
  5. ncbi Regulation of human neutrophil granule protein expression
    N Borregaard
    Department of Hematology, Rigshospitalet, University of Copenhagen, Denmark
    Curr Opin Hematol 8:23-7. 2001
  6. ncbi Neutrophil gelatinase-associated lipocalin, a siderophore-binding eukaryotic protein
    Niels Borregaard
    The Granulocyte Research Laboratory, Department of Hematology, University of Copenhagen, Rigshospitalet 4042, 9 Blegdamsvej, Copenhagen Ø, DK 2200, Denmark
    Biometals 19:211-5. 2006
  7. ncbi Neutrophils, from marrow to microbes
    Niels Borregaard
    The Granulocyte Research Laboratory, Department of Hematology, National University Hospital Rigshospitalet, University of Copenhagen, DK 2100 Copenhagen, Denmark
    Immunity 33:657-70. 2010
  8. ncbi Neutrophil granules: a library of innate immunity proteins
    Niels Borregaard
    The Granulocyte Research Laboratory, Department of Hematology, University of Copenhagen, Rigshospitalet, DK 2100, Denmark
    Trends Immunol 28:340-5. 2007
  9. ncbi [Centralization versus decentralization. Hematology]
    Niels Borregaard
    H S Rigshospitalet, Haematologisk Klinik, København Ø
    Ugeskr Laeger 168:1551-2. 2006
  10. ncbi Ficolin-1 is present in a highly mobilizable subset of human neutrophil granules and associates with the cell surface after stimulation with fMLP
    Sara Rørvig
    The Granulocyte Research Laboratory, Department of Hematology, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark
    J Leukoc Biol 86:1439-49. 2009

Detail Information

Publications105 found, 100 shown here

  1. doi What doesn't kill you makes you stronger: the anti-inflammatory effect of neutrophil respiratory burst
    Niels Borregaard
    Department of Hematology, National University Hospital, University of Copenhagen, 2100 Copenhagen, Denmark Electronic address
    Immunity 40:1-2. 2014
    ....
  2. doi Proteome profiling of human neutrophil granule subsets, secretory vesicles, and cell membrane: correlation with transcriptome profiling of neutrophil precursors
    Sara Rørvig
    2 Department of Hematology, University of Copenhagen, Rigshospitalet 9322, 20 Juliane Mariesvej, 2100 Copenhagen, Denmark
    J Leukoc Biol 94:711-21. 2013
    ..The neutrophil subcellular proteome profiles presented here may be used as a database in combination with the mRNA array database to predict and test the presence and localization of proteins in neutrophil granules and membranes. ..
  3. ncbi Neutrophils and keratinocytes in innate immunity--cooperative actions to provide antimicrobial defense at the right time and place
    Niels Borregaard
    Department of Hematology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    J Leukoc Biol 77:439-43. 2005
    ..This results in generation of signaling molecules, some of which support the growth and antimicrobial potential of keratinocytes and epithelial cells. This paper will highlight some recent advances in this field...
  4. ncbi Systems biology of neutrophil differentiation and immune response
    Kim Theilgaard-Monch
    The Granulocyte Research Laboratory, Department of Hematology 9322, Rigshospitalet, University of Copenhagen, Juliane Mariesvej 20, 2100 Copenhagen Ø, Denmark
    Curr Opin Immunol 18:54-60. 2006
    ..In addition, these studies provide evidence that neutrophil differentiation and immune response are governed by a highly coordinated transcriptional programme that regulates cellular fate and function in a context-dependent manner...
  5. ncbi Regulation of human neutrophil granule protein expression
    N Borregaard
    Department of Hematology, Rigshospitalet, University of Copenhagen, Denmark
    Curr Opin Hematol 8:23-7. 2001
    ..1 and C/EBPalpha stand out as critical for all granule proteins whereas AML-1 is critical for primary granule protein expression and C/EBPepsilon for secondary and tertiary granule protein expression...
  6. ncbi Neutrophil gelatinase-associated lipocalin, a siderophore-binding eukaryotic protein
    Niels Borregaard
    The Granulocyte Research Laboratory, Department of Hematology, University of Copenhagen, Rigshospitalet 4042, 9 Blegdamsvej, Copenhagen Ø, DK 2200, Denmark
    Biometals 19:211-5. 2006
    ..Megalin, a scavenger receptor functions as a receptor for NGAL and mediates uptake into endosomes, but other NGAL receptors are likely to exist...
  7. ncbi Neutrophils, from marrow to microbes
    Niels Borregaard
    The Granulocyte Research Laboratory, Department of Hematology, National University Hospital Rigshospitalet, University of Copenhagen, DK 2100 Copenhagen, Denmark
    Immunity 33:657-70. 2010
    ..As a final act, neutrophils can extrude stands of DNA with bactericidal proteins attached that act as extracellular traps for microorganisms...
  8. ncbi Neutrophil granules: a library of innate immunity proteins
    Niels Borregaard
    The Granulocyte Research Laboratory, Department of Hematology, University of Copenhagen, Rigshospitalet, DK 2100, Denmark
    Trends Immunol 28:340-5. 2007
    ..Likewise, it has become clear that some proteins previously thought to be specific to the neutrophil are expressed in epithelial cells during inflammation...
  9. ncbi [Centralization versus decentralization. Hematology]
    Niels Borregaard
    H S Rigshospitalet, Haematologisk Klinik, København Ø
    Ugeskr Laeger 168:1551-2. 2006
    ..The treatment of patients should be centralized in departments large enough to permit internal subspecialization and to provide expert service focused on haematology. No more than three such hematology centers are needed in Denmark...
  10. ncbi Ficolin-1 is present in a highly mobilizable subset of human neutrophil granules and associates with the cell surface after stimulation with fMLP
    Sara Rørvig
    The Granulocyte Research Laboratory, Department of Hematology, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark
    J Leukoc Biol 86:1439-49. 2009
    ..Our studies show that neutrophils are a major source of ficolin-1, which can be readily exocytosed by stimulation...
  11. ncbi The secretory leukocyte protease inhibitor (SLPI) and the secondary granule protein lactoferrin are synthesized in myelocytes, colocalize in subcellular fractions of neutrophils, and are coreleased by activated neutrophils
    Lars C Jacobsen
    The Granulocyte Research Laboratory, Department of Hematology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    J Leukoc Biol 83:1155-64. 2008
    ..These might include the protection of secondary GPs against proteolytic activation and/or degradation by proteases, which might be dislocated to secondary granules at minute amounts as a consequence of spillover...
  12. ncbi The transcriptional program of terminal granulocytic differentiation
    Kim Theilgaard-Monch
    The Granulocyte Research Laboratory, Department of Hematology, Rigshospitalet, University of Copenhagen, 2100 Copenhagen, Denmark
    Blood 105:1785-96. 2005
    ....
  13. ncbi Haptoglobin is synthesized during granulocyte differentiation, stored in specific granules, and released by neutrophils in response to activation
    Kim Theilgaard-Monch
    Granulocyte Research Laboratory Department of Hematology, Rigshospitalet, the Laboratory for Gene Therapy Research Department of Clinical Biochemistry, University of Copenhagen, Copenhagen Ø, Denmark
    Blood 108:353-61. 2006
    ..Hence, neutrophil-derived Hp might reduce tissue damage and bacterial growth at sites of infection or injury by propagating anti-inflammatory activities and Hb clearance...
  14. doi Serglycin participates in retention of α-defensin in granules during myelopoiesis
    Andreas Glenthøj
    The Granulocyte Research Laboratory, Department of Hematology, National University Hospital, University of Copenhagen, Copenhagen, Denmark
    Blood 118:4440-8. 2011
    ....
  15. doi Tethering of Ficolin-1 to cell surfaces through recognition of sialic acid by the fibrinogen-like domain
    Christian Honore
    Department of Clinical Immunology, Rigshospitalet, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark
    J Leukoc Biol 88:145-58. 2010
    ..Moreover, rFicolin-1 bound activated but not resting T lymphocytes. Together, these results demonstrate a novel self-recognition mechanism of leukocytes mediated by the fibrinogen-like domain of Ficolin-1...
  16. ncbi Identification of human cysteine-rich secretory protein 3 (CRISP-3) as a matrix protein in a subset of peroxidase-negative granules of neutrophils and in the granules of eosinophils
    Lene Udby
    Granulocyte Research Laboratory, Department of Hematology, Rigshospitalet, Copenhagen, Denmark
    J Leukoc Biol 72:462-9. 2002
    ..The presence of CRISP-3 in peroxidase-negative granules of neutrophils, in granules of eosinophils, and in exocrine secretions indicates a role in the innate host defense...
  17. pmc MicroRNA profiling in human neutrophils during bone marrow granulopoiesis and in vivo exudation
    Maria T Larsen
    The Granulocyte Research Laboratory, Department of Hematology, National University Hospital, University of Copenhagen, Copenhagen, Denmark
    PLoS ONE 8:e58454. 2013
    ..The data present the miRNA profiles during the development and activation of the neutrophil granulocyte in healthy humans and thus serves as a reference for further research of normal and malignant granulocytic development...
  18. pmc beta-Microseminoprotein binds CRISP-3 in human seminal plasma
    Lene Udby
    Granulocyte Research Laboratory, Department of Hematology, Copenhagen University Hospital, Rigshospitalet, Denmark
    Biochem Biophys Res Commun 333:555-61. 2005
    ..Structural similarity with an MSP-binding protein from blood plasma suggests that CRISP-3 binds MSP through its aminoterminal SCP-domain...
  19. pmc Olfactomedin 4 defines a subset of human neutrophils
    Stine N Clemmensen
    Granulocyte Research Laboratory, Rigshospitalet 9322, 20 Juliane Maries vej, Copenhagen, Denmark
    J Leukoc Biol 91:495-500. 2012
    ....
  20. ncbi Highly glycosylated alpha1-acid glycoprotein is synthesized in myelocytes, stored in secondary granules, and released by activated neutrophils
    Kim Theilgaard-Monch
    Granulocyte Research Laboratory, Department of Hematology, Rigshospitalet, University of Copenhagen, Blegdamsvej 9, 2100 Copenhagen Ø, Denmark
    J Leukoc Biol 78:462-70. 2005
    ..Hence, neutrophils, which constitute the first line of defense, are likely to serve as the primary local source of AGP at sites of infection or injury...
  21. pmc Lipocalin 2 is protective against E. coli pneumonia
    Hong Wu
    Granulocyte Research Laboratory, Rigshospitalet, Copenhagen, Denmark
    Respir Res 11:96. 2010
    ..coli is rare despite a frequent exposure to this commensal bacterium. Lipocalin 2 is an effector molecule of the innate immune system and could therefore play a role in hindering growth of E. coli in the lungs...
  22. ncbi IL-1beta-specific up-regulation of neutrophil gelatinase-associated lipocalin is controlled by IkappaB-zeta
    Jack B Cowland
    Department of Hematology L 9322, Granulocyte Research Laboratory, Rigshospitalet, University of Copenhagen, 9 Blegdamsvej, DK 2100 Copenhagen, Denmark
    J Immunol 176:5559-66. 2006
    ..Our findings indicate that a common regulatory mechanism has evolved to control expression of a subset of antimicrobial proteins expressed in epithelial cells...
  23. ncbi Cysteine-rich secretory protein 3 is a ligand of alpha1B-glycoprotein in human plasma
    Lene Udby
    Granulocyte Research Laboratory, Department of Hematology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
    Biochemistry 43:12877-86. 2004
    ..We suggest that the A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP-3...
  24. doi Human CRISP-3 binds serum alpha(1)B-glycoprotein across species
    Lene Udby
    Granulocyte Research Laboratory, Department of Hematology, Copenhagen University Hospital, Rigshospitalet, Denmark
    Biochim Biophys Acta 1800:481-5. 2010
    ..Since animal sera are often used to supplement buffers in experiments, in particular such that involve cell cultures, binding proteins present in sera might interfere in the experiments...
  25. ncbi Arginase 1 is expressed in myelocytes/metamyelocytes and localized in gelatinase granules of human neutrophils
    Lars C Jacobsen
    Granulocyte Research Laboratory, Department of Hematology, Rigshospitalet University Hospital, Copenhagen, Denmark
    Blood 109:3084-7. 2007
    ..Overall, these findings define ARG1 as a genuine gelatinase granule protein and support a model in which activated neutrophils release ARG1 at sites of infection to modulate immune responses and promote tissue regeneration...
  26. ncbi Wound healing and expression of antimicrobial peptides/polypeptides in human keratinocytes, a consequence of common growth factors
    Ole E Sørensen
    Granulocyte Research Laboratory, Department of Hematology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
    J Immunol 170:5583-9. 2003
    ..These findings offer an explanation for the expression of these peptides/polypeptides in the skin disease psoriasis and in wound healing and define a host defense role for growth factors in wound healing...
  27. ncbi Characterization and localization of cysteine-rich secretory protein 3 (CRISP-3) in the human male reproductive tract
    Lene Udby
    Granulocyte Research Laboratory 9322, Department of Hematology, Copenhagen University Hospital, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen Ø, Denmark
    J Androl 26:333-42. 2005
    ..Examination of seminal plasma from vasectomized males indicates that organs downstream of the epididymis are probably the major sources of seminal plasma CRISP-3...
  28. ncbi Prodefensins are matrix proteins of specific granules in human neutrophils
    Mikkel Faurschou
    Department of Hematology, Rigshospitalet 4042, DK 2100 Copenhagen, Denmark
    J Leukoc Biol 78:785-93. 2005
    ..Thus, human neutrophils not only store large amounts of mature defensins in azurophil granules but also contain a more easily mobilized reservoir of unprocessed prodefensins in specific granules...
  29. ncbi Neutrophil elastase depends on serglycin proteoglycan for localization in granules
    Carsten U Niemann
    Rigshospitalet, Department of Hematology, Granulocyte Research Laboratory, University of Copenhagen, Copenhagen, Denmark
    Blood 109:4478-86. 2007
    ..Thus, serglycin proteoglycan has an important role in localizing neutrophil elastase in azurophil granules of neutrophils, while localization of other granule proteins must be mediated by other mechanisms...
  30. doi Technical advance: immunophenotypical characterization of human neutrophil differentiation
    Helena Mora-Jensen
    Department of Hematology, University of Copenhagen, 2200 Copenhagen N, Denmark
    J Leukoc Biol 90:629-34. 2011
    ....
  31. pmc Induction of neutrophil gelatinase-associated lipocalin expression by co-stimulation with interleukin-17 and tumor necrosis factor-alpha is controlled by IkappaB-zeta but neither by C/EBP-beta nor C/EBP-delta
    Joachim R Karlsen
    Department of Hematology 93 2 2, Granulocyte Research Laboratory, University of Copenhagen, Rigshospitalet, 9 Blegdamsvej, DK 2100 Copenhagen, Denmark
    J Biol Chem 285:14088-100. 2010
    ..Co-stimulation with IL-17 leads to accumulation of IkappaB-zeta mRNA and IkappaB-zeta protein, which can bind to NF-kappaB on the LCN2 promoter and thus induce NGAL expression...
  32. doi Serglycin proteoglycan is not implicated in localizing exocrine pancreas enzymes to zymogen granules
    Carsten U Niemann
    Department of Haematology, Rigshospitalet, The Granulocyte Research Laboratory, Building 9322, Blegdamsvej 9, DK 2100 Copenhagen, Denmark
    Eur J Cell Biol 88:473-9. 2009
    ..Furthermore, the amount and localization of three exocrine pancreas zymogens (amylase, trypsinogen, and carboxypeptidase A) is not affected by the absence of serglycin in a serglycin knock-out mouse model...
  33. ncbi End-stage differentiation of neutrophil granulocytes in vivo is accompanied by up-regulation of p27kip1 and down-regulation of CDK2, CDK4, and CDK6
    Pia Klausen
    The Granulocyte Research Laboratory, Department of Hematology, Rigshospitalet, University of Copenhagen, Denmark
    J Leukoc Biol 75:569-78. 2004
    ..This is the first report to describe expression levels of cell-cycle proteins during granulopoiesis in vivo, and it strongly contrasts the observations made in cell-culture systems in vitro...
  34. ncbi Localization of serglycin in human neutrophil granulocytes and their precursors
    Carsten Utoft Niemann
    Rigshospitalet, Department of Haematology, Granulocytlaboratoriet, Building 9322, Blegdamsvej 9, DK 2100, Copenhagen, Denmark
    J Leukoc Biol 76:406-15. 2004
    ..Our findings contrast the view that native serglycin is present in mature granules and plays a role in packaging and regulating the activity of proteolytic enzymes there...
  35. ncbi The transcriptional activation program of human neutrophils in skin lesions supports their important role in wound healing
    Kim Theilgaard-Monch
    The Granulocyte Research Laboratory, Department of Hematology 9322, Rigshospitalet, University of Copenhagen, Blegdamsvej 9, DK 2100 Copenhagen, Denmark
    J Immunol 172:7684-93. 2004
    ..These findings demonstrate that migration of PMNs to skin lesions induces a transcriptional activation program, which regulates cellular fate and function, and promotes wound healing...
  36. pmc No effect of NGAL/lipocalin-2 on aggressiveness of cancer in the MMTV-PyMT/FVB/N mouse model for breast cancer
    Elisabeth P Cramer
    The Granulocyte Research Laboratory, Department of Hematology, Copenhagen University Hospital, Copenhagen, Denmark
    PLoS ONE 7:e39646. 2012
    ..We conclude that NGAL/lipocalin-2 does not invariably affect the aggressiveness of breast cancers as assessed in mouse models, thus questioning the role of lipocalin-2 in cancer development...
  37. ncbi On mouse and man: neutrophil gelatinase associated lipocalin is not involved in apoptosis or acute response
    Pia Klausen
    Department of Hematology, The Granulocyte Research Laboratory, Rigshospitalet 9322, 9 Blegdamsvej, DK 2100 Copenhagen, Denmark
    Eur J Haematol 75:332-40. 2005
    ..We thus conclude that major differences exist between mouse and man with regards to the role of this lipocalin in myelopoiesis and inflammation...
  38. doi Alpha-1-antitrypsin is produced by human neutrophil granulocytes and their precursors and liberated during granule exocytosis
    Stine N Clemmensen
    The Granulocyte Research Laboratory, Department of Hematology, National University Hospital, Copenhagen, Denmark
    Eur J Haematol 86:517-30. 2011
    ..Impaired binding of neutrophil A1AT to serine proteases in patients with (PI)ZZ mutations may enhance their susceptibility to the development of emphysema...
  39. ncbi Neutrophil gelatinase-associated lipocalin is up-regulated in human epithelial cells by IL-1 beta, but not by TNF-alpha
    Jack B Cowland
    Department of Hematology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    J Immunol 171:6630-9. 2003
    ..Together, this demonstrates a selective up-regulation of NGAL by the IL-1 pathway...
  40. ncbi Derangement of transcription factor profiles during in vitro differentiation of HL60 and NB4 cells
    Malene Bjerregaard Pass
    Granulocyte Research Laboratory, Department of Hematology 93 2 2, Rigshospitalet, University of Copenhagen, Blegdamsvej 9, DK 2100 Copenhagen, Denmark
    Leuk Res 31:827-37. 2007
    ..These data illustrate the limitations of cell lines as models for normal granulopoiesis...
  41. ncbi Interactions between neutrophil gelatinase-associated lipocalin and natural lipophilic ligands
    T Bratt
    The Granulocyte Research Laboratory, Department of Hematology, Finsen Centre, Rigshospitalet, Copenhagen, Denmark
    Biochim Biophys Acta 1472:262-9. 1999
    ..The results demonstrate the superiority of weak affinity chromatography as a method of studying the interactions of the specific granule protein NGAL...
  42. ncbi Free-flow electrophoresis in subcellular fractionation of human neutrophils
    H Sengelov
    The Granulocyte Research Laboratory 9322, National University Hospital, Rigshospitalet, Blegdamsvej 9, DK 2100, Copenhagen, Denmark
    J Immunol Methods 232:145-52. 1999
    ..Methodological considerations and results obtained by FFE of light membranes from human neutrophils are presented in this paper...
  43. ncbi Prognostic significance of metallothionein in B-cell lymphomas
    Christian Bjørn Poulsen
    Department of Pathology 5444, Copenhagen University Hospital, Rigshospitalet, Blegdamsvej 9, 2100 Kbh Ø, Denmark
    Blood 108:3514-9. 2006
    ..Taken together, it is suggested that both increased MT mRNA and MT protein expression by more than 20% lymphoma cells constitute independent risk factors in DLBCL...
  44. ncbi Defensin-rich granules of human neutrophils: characterization of secretory properties
    Mikkel Faurschou
    The Granulocyte Research Laboratory, Department of Hematology, The National University Hospital, Rigshospitalet, Juliane Maries Vej 20, DK 2100 OE Copenhagen, Denmark
    Biochim Biophys Acta 1591:29-35. 2002
    ..Thus, in contrast to peroxidase-negative granules, azurophil granules display homogeneity in their availability for extracellular release...
  45. doi MicroRNA-130a-mediated down-regulation of Smad4 contributes to reduced sensitivity to TGF-β1 stimulation in granulocytic precursors
    Mattias Häger
    Granulocyte Research Laboratory, Department of Haematology, Rigshospitalet, Blegdamsvej 9, Copenhagen, Denmark
    Blood 118:6649-59. 2011
    ..Our data indicate that miR-130a is involved in cell cycle regulation of granulocytic cells through engagement of Smad4 in the TGF-β pathway...
  46. ncbi hCAP-18, a cathelin/pro-bactenecin-like protein of human neutrophil specific granules
    J B Cowland
    Department of Hematology, National University Hospital, Copenhagen, Denmark
    FEBS Lett 368:173-6. 1995
    ..Thus, the 19 kDa protein is hCAP-18, stored as a 'pro-peptide' in specific granules...
  47. ncbi Molecular characterization and pattern of tissue expression of the gene for neutrophil gelatinase-associated lipocalin from humans
    J B Cowland
    Granulocyte Research Laboratory, Finsen Center, Department of Hematology, Rigshospitalet, Copenhagen University Hospital, Denmark
    Genomics 45:17-23. 1997
    ..1, and the LPS-inducible factor NF-kappa B...
  48. doi ProHNPs are the principal α-defensins of human plasma
    Andreas Glenthøj
    The Granulocyte Research Laboratory, Department of Hematology, National University Hospital, Copenhagen, Denmark
    Eur J Clin Invest 43:836-43. 2013
    ..Numerous studies have investigated HNPs in plasma, linking them to a variety of diseases, but without distinguishing between HNPs and their proforms...
  49. ncbi Tissue expression of copines and isolation of copines I and III from the cytosol of human neutrophils
    Jack B Cowland
    Department of Hematology, Rigshospitalet, Copenhagen, Denmark
    J Leukoc Biol 74:379-88. 2003
    ....
  50. ncbi Neutrophil granules and secretory vesicles in inflammation
    Mikkel Faurschou
    The Granulocyte Research Laboratory, Department of Haematology, Rigshospitalet 4042, University of Copenhagen, 9 Blegdamsvej, DK 2100 Copenhagen OE, Denmark
    Microbes Infect 5:1317-27. 2003
    ..This review summarises current knowledge of granule biology and highlights the effects of neutrophil degranulation in the acute inflammatory response...
  51. ncbi The genetics of ficolins
    Peter Garred
    Laboratory of Molecular Medicine, Department of Clinical Immunology, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark garred post5 tele dk
    J Innate Immun 2:3-16. 2010
    ..This FCN3 variation was also shown to be associated with immunodeficiency. This survey summarizes the current phylogenetic and inter-individual molecular understanding of the FCN genes...
  52. ncbi SGP28, a novel matrix glycoprotein in specific granules of human neutrophils with similarity to a human testis-specific gene product and a rodent sperm-coating glycoprotein
    L Kjeldsen
    Granulocyte Research Laboratory, Department of Hematology, The Finsen Center, Rigshospitalet, Denmark
    FEBS Lett 380:246-50. 1996
    ..Subcellular fractionation of human neutrophils indicated that the protein is localized in specific granules. The protein was named SGP28 (specific granule protein of 28 kDa)...
  53. ncbi Molecular cloning and expression of a cDNA encoding NGAL: a lipocalin expressed in human neutrophils
    J R Bundgaard
    Department of Clinical Biochemistry, Rigshospitalet, University Hospital, Copenhagen, Denmark
    Biochem Biophys Res Commun 202:1468-75. 1994
    ..A faint signal was observed in fetal and adult human lung tissue. The molecular cloning of the NGAL cDNA allowed the recombinant production of NGAL in E. coli...
  54. doi The innate pattern recognition molecule Ficolin-1 is secreted by monocytes/macrophages and is circulating in human plasma
    Christian Honore
    Department of Clinical Immunology, Rigshospitalet, Blegdamsvej 9, Copenhagen, Denmark
    Mol Immunol 45:2782-9. 2008
    ..Importantly, these results demonstrate that Ficolin-1 exists in human plasma and serum under normal conditions, hereby revising the general assumption that Ficolin-1 is solely a cellular associated protein...
  55. ncbi Microarray-based classification of diffuse large B-cell lymphoma
    Christian Bjørn Poulsen
    Department of Pathology, Copenhagen University Hospital, Rigshospitalet, Denmark
    Eur J Haematol 74:453-65. 2005
    ....
  56. ncbi Subcellular fractionation of human neutrophils and analysis of subcellular markers
    Lene Udby
    Department of Hematology, Rigshospitalet, Copenhagen University, Copenhagen, Denmark
    Methods Mol Biol 412:35-56. 2007
    ..Immunological methods (sandwich enzyme-linked immunosorbent assays) are used for quantitation of proteins that are characteristic constituents of the granule subsets of neutrophils...
  57. pmc Capacitance flickers and pseudoflickers of small granules, measured in the cell-attached configuration
    K Lollike
    The Granulocyte Research Laboratory, Department of Hematology, The Finsen Centre, Rigshospitalet, Copenhagen, Denmark
    Biophys J 75:53-9. 1998
    ..We conclude that non-stepwise capacitance changes must be interpreted with caution, since a number of factors go into determining cell or patch admittance...
  58. ncbi Neutrophil beta-2 microglobulin: an inflammatory mediator
    O W Bjerrum
    Department of Medicine and Haematology L, State University Hospital, Copenhagen, Denmark
    Scand J Immunol 32:233-42. 1990
    ..We suggest that neutrophil beta 2m following exocytosis may be transformed to Des-Lys58-beta 2m, acting as an extracellular messenger between granulocytes and lymphocytes in the inflammatory focus...
  59. ncbi Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase
    L Kjeldsen
    Departments of Hematology, University Hospital, Rigshospitalet, Copenhagen, Denmark
    J Biol Chem 268:10425-32. 1993
    ..It is thus a new member of the lipocalin family. The function of the 25-kDa protein, named neutrophil gelatinase-associated lipocalin (NGAL), remains to be determined...
  60. ncbi The individual regulation of granule protein mRNA levels during neutrophil maturation explains the heterogeneity of neutrophil granules
    J B Cowland
    The Finsen Centre, Department of Hematology, Rigshospitalet, University of Copenhagen, Denmark
    J Leukoc Biol 66:989-95. 1999
    ....
  61. ncbi Subcellular fractionation of human neutrophils on Percoll density gradients
    L Kjeldsen
    Granulocyte Research Laboratory, Department of Hematology, The Finsen Center, The National University Hospital, Rigshospitalet, 9 Blegdamsvej, 2100, Copenhagen, Denmark
    J Immunol Methods 232:131-43. 1999
    ....
  62. ncbi Serglycin proteoglycan in hematologic malignancies: a marker of acute myeloid leukemia
    C U Niemann
    Department of Hematology, Rigshospitalet, Copenhagen, Denmark
    Leukemia 21:2406-10. 2007
    ..In contrast to myeloperoxidase, serglycin was found to be a selective marker for immature myeloid cells, distinguishing AML from Philadelphia chromosome-negative chronic myeloproliferative disorders...
  63. ncbi The human antibacterial cathelicidin, hCAP-18, is bound to lipoproteins in plasma
    O Sørensen
    Granulocyte Research Laboratory, Department of Hematology, The Finsen Centre, National University Hospital, DK 2100 Copenhagen O, Denmark
    J Biol Chem 274:22445-51. 1999
    ..The binding of hCAP-18 to lipoproteins suggests that lipoproteins may play an important role as a reservoir of this antimicrobial protein...
  64. ncbi Giant granules of neutrophils in Chediak-Higashi syndrome are derived from azurophil granules but not from specific and gelatinase granules
    L Kjeldsen
    Department of Hematology, The National University Hospital, Copenhagen, Denmark
    J Leukoc Biol 64:72-7. 1998
    ..In conclusion, giant granules from CHS neutrophils originate from azurophil granules but not from the specific and gelatinase granules...
  65. ncbi Methods for quantitation of human neutrophil proteins, a survey
    O Sørensen
    The Granulocyte Research Laboratory, Department of Hematology L 9322, The Finsen Centre, The National University Hospital, Rigshospitalet, 9 Blegdamsvej, DK 2100, Copenhagen, Denmark
    J Immunol Methods 232:179-90. 1999
    ....
  66. ncbi Isolation of neutrophil precursors from bone marrow for biochemical and transcriptional analysis
    J B Cowland
    The Granulocyte Research Laboratory, The Finsen Centre, Department of Hematology, Rigshospitalet L 9322, 9 Blegdamsvej, University of Copenhagen, DK 2100, Copenhagen, Denmark
    J Immunol Methods 232:191-200. 1999
    ....
  67. ncbi Human cathelicidin, hCAP-18, is processed to the antimicrobial peptide LL-37 by extracellular cleavage with proteinase 3
    O E Sørensen
    Granulocyte Research Laboratory, Department of Hematology, Copenhagen University Hospital, 9 Blegdamsvej, DK 2100 Copenhagen, Denmark
    Blood 97:3951-9. 2001
    ..Blood. 2001;97:3951-3959)..
  68. ncbi Human neutrophil gelatinase-associated lipocalin and homologous proteins in rat and mouse
    L Kjeldsen
    The Granulocyte Research Laboratory, Department of Hematology, The National University Hospital, L 4042, Rigshospitalet, 9 Blegdamsvej, DK 2100 Copenhagen, Denmark
    Biochim Biophys Acta 1482:272-83. 2000
    ..This may indicate either a microbicidal activity of NGAL or a role in regulation of inflammation or cellular growth, putative functions yet to be demonstrated...
  69. ncbi Patient education--a strategy for prevention of infections caused by permanent central venous catheters in patients with haematological malignancies: a randomized clinical trial
    T Møller
    Department of Haematology, Rigshospitalet, Copenhagen, Denmark
    J Hosp Infect 61:330-41. 2005
    ..We conclude that systematic individualized, supervised patient education is able to reduce catheter-related infections...
  70. ncbi Profiling of gene expression in individual hematopoietic cells by global mRNA amplification and slot blot analysis
    K Theilgaard-Monch
    The Granulocyte Research Laboratory, Department of Hematology 9322, The Finsen Center, Rigshospitalet, University of Copenhagen, Blegdamsvej 9, 2100, Copenhagen, Denmark
    J Immunol Methods 252:175-89. 2001
    ..This study demonstrates the potential of cDNA, synthesized from individual cells by global amplification of mRNA, as probes for cDNA arrays...
  71. pmc Human α-defensin expression is not dependent on CCAAT/enhancer binding protein-ε in a murine model
    Andreas Glenthøj
    The Granulocyte Research Laboratory, Department of Hematology, National University Hospital, University of Copenhagen, Copenhagen, Denmark
    PLoS ONE 9:e92471. 2014
    ..Taken together, our data infer that the HNP-1 of the transgenic mouse does not show an expression pattern equivalent to endogenous secondary granule proteins. This limits the use of these transgenic mice as a model for human conditions. ..
  72. ncbi Compound exocytosis of granules in human neutrophils
    Karsten Lollike
    The Granulocyte Research Laboratory, Department of Hematology, Rigshospitalet, Copenhagen, Denmark
    J Leukoc Biol 71:973-80. 2002
    ..Compound exocytosis may be a mechanism for efficient targeting of release during exocytosis...
  73. ncbi Processing of seminal plasma hCAP-18 to ALL-38 by gastricsin: a novel mechanism of generating antimicrobial peptides in vagina
    Ole E Sørensen
    Granulocyte Research Laboratory, The Department of Hematology, Copenhagen University Hospital, Rigshospitalet, DK 2100 Copenhagen, Denmark
    J Biol Chem 278:28540-6. 2003
    ..This enzymatic activation of a proantimicrobial substance in seminal plasma following exposure to the vaginal milieu represents a novel mechanism to prevent infection following sexual intercourse...
  74. ncbi An ELISA for SGP28/CRISP-3, a cysteine-rich secretory protein in human neutrophils, plasma, and exocrine secretions
    Lene Udby
    Granulocyte Research Laboratory, Department of Hematology L 9322, Rigshospitalet, 9 Blegdamsvej, Copenhagen, Denmark
    J Immunol Methods 263:43-55. 2002
    ..8 microg/ml), seminal plasma (11.2 microg/ml), and sweat (0.15 microg/ml), and describe the coexistence of two different molecular weight forms of CRISP-3, representing an N-glycosylated and a non-glycosylated form of the mature protein...
  75. ncbi The in vivo profile of transcription factors during neutrophil differentiation in human bone marrow
    Malene Digmann Bjerregaard
    Department of Hematology, Rigshospitalet, University of Copenhagen, Denmark
    Blood 101:4322-32. 2003
    ..This demonstrates a highly individualized expression of the transcription factors, which can form the basis for the heterogeneous expression of granule proteins during granulopoiesis and cell cycle arrest in metamyelocytes...
  76. pmc Identification of a highly mobilizable subset of human neutrophil intracellular vesicles that contains tetranectin and latent alkaline phosphatase
    N Borregaard
    Department of Internal Medicine, Righospitalet, Copenhagen, Denmark
    J Clin Invest 85:408-16. 1990
    ..These organelles may play an important role as stores of membrane proteins that are mobilized to the cell surface during stimulation by inflammatory mediators...
  77. ncbi Neutrophil granules in health and disease
    M Hager
    Granulocyte Research Laboratory, Department of Haematology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    J Intern Med 268:25-34. 2010
    ..This is largely controlled by gene transcription...
  78. doi Subcellular fractionation of human neutrophils and analysis of subcellular markers
    Stine Novrup Clemmensen
    The Granulocyte Research Laboratory, Department of Hematology, Rigshospitalet, Copenhagen University, Copenhagen, Denmark
    Methods Mol Biol 1124:53-76. 2014
    ..Immunological methods (sandwich enzyme-linked immunosorbent assays) are used for quantitation of proteins that are characteristic constituents of the granule subsets of neutrophils. ..
  79. ncbi Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner
    K Lollike
    Granulocyte Research Laboratory, Department of Hematology, Rigshospitalet, 2100 Copenhagen, Denmark
    J Biol Chem 276:17762-9. 2001
    ..This was substantiated by co-immunoprecipitation of grancalcin by anti-L-plastin antibodies and vice versa...
  80. ncbi Human neutrophils are devoid of the integral membrane protein caveolin
    H Sengeløv
    Department of Hematology, National University Hospital, Copenhagen, Denmark
    J Leukoc Biol 63:563-6. 1998
    ..We conclude that human neutrophils are devoid of caveolin and that secretory vesicles are not related to caveolae nor dependent on caveolae for mobilization...
  81. doi A conceptual framework for the identification of candidate drugs and drug targets in acute promyelocytic leukemia
    T T Marstrand
    Biotech Research and Innovation Center, University of Copenhagen, Copenhagen, Denmark
    Leukemia 24:1265-75. 2010
    ....
  82. ncbi The neutrophil lipocalin NGAL is a bacteriostatic agent that interferes with siderophore-mediated iron acquisition
    David H Goetz
    Molecular and Cellular Biology Program, Fred Hutchinson Cancer Research Center, Box 357275, University of Washington, Seattle, WA 98105, USA
    Mol Cell 10:1033-43. 2002
    ..We therefore propose that NGAL participates in the antibacterial iron depletion strategy of the innate immune system...
  83. ncbi Phosphorylation of Bax Ser184 by Akt regulates its activity and apoptosis in neutrophils
    Shyra J Gardai
    Program in Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, CO 80206, USA
    J Biol Chem 279:21085-95. 2004
    ....
  84. ncbi The cathelicidin anti-microbial peptide LL-37 is involved in re-epithelialization of human skin wounds and is lacking in chronic ulcer epithelium
    Johan D Heilborn
    Department of Dermatology, Karolinska Institute, Stockholm, Sweden
    J Invest Dermatol 120:379-89. 2003
    ..Thus, we suggest that, in addition to being an anti-microbial peptide, LL-37 also plays a part in wound closure and that its reduction in chronic wounds impairs re-epithelialization and may contribute to their failure to heal...
  85. ncbi Neutrophil gelatinase-associated lipocalin is a marker for dysregulated keratinocyte differentiation in human skin
    Lotus Mallbris
    Department of Dermatology, Karolinska Hospital, Stockholm, Sweden
    Exp Dermatol 11:584-91. 2002
    ..In addition, the transient interfollicular NGAL expression during skin embryogenesis along with the induction of NGAL in adult parakeratotic epidermis suggests it play a role in epithelial differentiation pathways...
  86. ncbi Phospholipid flip-flop and phospholipid scramblase 1 (PLSCR1) co-localize to uropod rafts in formylated Met-Leu-Phe-stimulated neutrophils
    S Courtney Frasch
    Department of Pediatrics, Division of Cell Biology, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    J Biol Chem 279:17625-33. 2004
    ..Of note, PS exposure, phospholipid uptake, and FM 1-43 staining also localized to the uropod following stimulation demonstrating that both PLSCR1 and phospholipid flip-flop characterize this specialized domain of polarized neutrophils...
  87. ncbi Identification of the presenilins in hematopoietic cells with localization of presenilin 1 to neutrophil and platelet granules
    Zeljka Korade Mirinics
    Department of Pediatrics, University of Pittsburgh School of Medicine, Pennsylvania 15261, USA
    Blood Cells Mol Dis 28:28-38. 2002
    ..These studies indicate that PS1 may play multiple roles in blood cell physiology and that blood tissue may provide a model to study PS1 interactions with other proteins...
  88. ncbi Antimicrobial protein hCAP18/LL-37 is highly expressed in breast cancer and is a putative growth factor for epithelial cells
    Johan D Heilborn
    Unit of Dermatology, Department of Medicine, Karolinska Institutet, Stockholm, Sweden
    Int J Cancer 114:713-9. 2005
    ..These findings do not support the hypothesis that LL-37 has an antitumor effect, but rather suggest that hCAP18/LL-37 may promote tumor cell growth in breast cancer...
  89. ncbi Expression of neutrophil gelatinase-associated lipocalin in skin epidermis
    Seong Jun Seo
    J Invest Dermatol 126:510-2. 2006
  90. ncbi Neutrophil gelatinase-associated lipocalin (NGAL) an early-screening biomarker for ovarian cancer: NGAL is associated with epidermal growth factor-induced epithelio-mesenchymal transition
    Ratana Lim
    Gynaecological Cancer Research Centre, Royal Women s Hospital, Carlton, Victoria, Australia
    Int J Cancer 120:2426-34. 2007
    ..These data indicate that NGAL may be a good marker to monitor changes of benign to premalignant and malignant ovarian tumors and that the molecule may be involved in the progression of epithelial ovarian malignancies...
  91. ncbi Lysophospholipids of different classes mobilize neutrophil secretory vesicles and induce redundant signaling through G2A
    S Courtney Frasch
    Department of Pediatrics, Division of Cell Biology, National Jewish Medical and Research Center, Denver, CO 80206, USA
    J Immunol 178:6540-8. 2007
    ..Together, these data show that lyso-PLs bearing various head groups redundantly mobilize G2A latent within secretory vesicles and result in G2A receptor/Galphai/phospholipase C signaling for calcium flux in neutrophils...
  92. ncbi Human cathelicidin antimicrobial peptide (CAMP) gene is a direct target of the vitamin D receptor and is strongly up-regulated in myeloid cells by 1,25-dihydroxyvitamin D3
    Adrian F Gombart
    Department of Medicine, Division of Hematology Oncology, Cedars Sinai Medical Center, David Geffen School of Medicine at UCLA, Los Angeles, California 90048, USA
    FASEB J 19:1067-77. 2005
    ..Our findings reveal a novel activity of 1,25-dihydroxyvitamin D3 and the VDR in regulation of primate innate immunity...
  93. ncbi Low salivary dehydroepiandrosterone and androgen-regulated cysteine-rich secretory protein 3 levels in Sjögren's syndrome
    Mikael Laine
    Helsinki University Central Hospital, and University of Helsinki, FIN 00029 HUS, Helsinki, Finland
    Arthritis Rheum 56:2575-84. 2007
    ..We undertook this study to test the hypothesis that SS is characterized by an insufficient androgen effect at the target tissue level...
  94. ncbi Protein-tyrosine phosphatase MEG2 is expressed by human neutrophils. Localization to the phagosome and activation by polyphosphoinositides
    Joshua M Kruger
    Division of Respirology, The Toronto General Hospital Research Institute of the University Health Network, Toronto, Ontario M5S 1A8, Canada
    J Biol Chem 277:2620-8. 2002
    ..Taken together, our results indicate that MEG2 is a polyphosphoinositide-activated tyrosine phosphatase that may be involved in signaling events regulating phagocytosis, an essential antimicrobial function in the innate immune response...
  95. ncbi The antimicrobial peptide LL-37 activates innate immunity at the airway epithelial surface by transactivation of the epidermal growth factor receptor
    G Sandra Tjabringa
    Department of Pulmonology, Leiden University Medical Center, Leiden, The Netherlands
    J Immunol 171:6690-6. 2003
    ..LL-37 may thus constitute one of the mediators by which neutrophils regulate epithelial cell activity in the lung...
  96. ncbi Urinary neutrophil gelatinase-associated lipocalin and acute kidney injury after cardiac surgery
    Gebhard Wagener
    Department of Anesthesiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032 3784, USA
    Am J Kidney Dis 52:425-33. 2008
    ..We report the association of urinary NGAL with indexes of intraoperative renal hypoperfusion (cardiopulmonary bypass time and aortic cross-clamp time) and acute kidney injury (AKI) after adult cardiac surgery...
  97. ncbi Protein kinase R mediates intestinal epithelial gene remodeling in response to double-stranded RNA and live rotavirus
    Matam Vijay-Kumar
    Department of Pathology and Laboratory Medicine, Epithelial Pathobiology Unit, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Immunol 174:6322-31. 2005
    ....
  98. ncbi Groups IV, V, and X phospholipases A2s in human neutrophils: role in eicosanoid production and gram-negative bacterial phospholipid hydrolysis
    Norbert Degousee
    Division of Vascular Surgery, Max Bell Research Center, Toronto General Hospital, University Health Network, Toronto M5G 2C4, Canada
    J Biol Chem 277:5061-73. 2002
    ....
  99. ncbi The endocytic receptor megalin binds the iron transporting neutrophil-gelatinase-associated lipocalin with high affinity and mediates its cellular uptake
    Vibeke Hvidberg
    Institute of Medical Biochemistry, University of Aarhus, Denmark
    FEBS Lett 579:773-7. 2005
    ..Furthermore, a rat yolk sac cell line known to express high levels of megalin, endocytosed NGAL by a mechanism completely blocked by an antibody against megalin...
  100. ncbi Cathelicidins--nature's attempt at combinatorial chemistry
    Ole E Sorensen
    Department of Cell and Molecular Biology, Section ofMolecular Pathogenesis, Lund University, Sweden
    Comb Chem High Throughput Screen 8:273-80. 2005
    ..However, one of the major challenges will be to screen these peptides in experimentally relevant models that reflect the environments in which the peptides should be therapeutically active...