- Holm M, Andersen H, Hetland T, Dahl C, Hoffmann H, Junker S, et al. Seven week culture of functional human mast cells from buffy coat preparations. J Immunol Methods. 2008;336:213-21 pubmed publisher..g. bone marrow or cord blood-derived progenitors. Consequently, our protocol has great potential in studies on mast cell biology and mast cell pathology, and e.g. on evaluation of drug effects. ..