Research Topics
Genomes and GenesSpecies | Peter HoklandSummaryAffiliation: Aarhus University Hospital Country: Denmark Publications
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Publications
Sensitivity of minimal residual disease in acute myeloid leukaemia in first remission--methodologies in relation to their clinical situationPeter Hokland
Department of Haematology, Aarhus University Hospital, Aarhus, Denmark
Br J Haematol 158:569-80. 2012..Here we will attempt to give an overview of these pertinent questions intended for the practicing haematologist, focusing on where the field is heading at the clinical level...
[Minimal residual disease in malignant diseases of the blood I. Background and pre-clinical validation]Peter Hokland
Immunhaematologisk Laboratorium, Haematologisk Afdeling R, Arhus Universitetshospital, Arhus Sygehus, DK 8000 Arhus C
Ugeskr Laeger 171:229-31. 2009..The sensitivity of quantitative polymerase chain reaction typically reaches a validated identification of 1 malignant cell in 100,000...- [Minimal residual disease in malignant diseases of the blood II. Translation and therapeutic consequences]Peter Hokland
Immunhaematologisk Laboratorium, Haematologisk Afdeling R, Arhus Universitetshospital, Arhus Sygehus, DK 8000 Arhus C
Ugeskr Laeger 171:232-5. 2009..A special point in favour of the techniques is the powerful and early detection of disease relapses, in some cases up to one year prior to clinical detection... - Towards individualized follow-up in adult acute myeloid leukemia in remissionPeter Hokland
Department of Haematology, Aarhus University Hospital, Denmark
Blood 117:2577-84. 2011.... - The combined expression of HOXA4 and MEIS1 is an independent prognostic factor in patients with AMLMike Zangenberg
Department of Haematology, Aarhus University Hospital, 8000 Aarhus C, Denmark
Eur J Haematol 83:439-48. 2009..In conclusion, our data show that the combination of low HOXA4 and low MEIS1 gene expression is a favourable predictor for outcome in all AML patients and that the expression levels are governed by the methylation state of these genes... - Strikingly different molecular relapse kinetics in NPM1c, PML-RARA, RUNX1-RUNX1T1, and CBFB-MYH11 acute myeloid leukemiasHans Beier Ommen
Laboratory of Immunohematology, Department of Hematology, Aarhus University Hospital, Aarhus, Denmark
Blood 115:198-205. 2010..These data carry important implications for the development of optimal RQ-PCR monitoring schedules suitable for evaluation of minimal residual disease-directed therapies in future clinical trials... - Molecular typing of adult acute myeloid leukaemia: significance of translocations, tandem duplications, methylation, and selective gene expression profilingLene Hyldahl Olesen
Department of Haematology, Aarhus University Hospital, Aarhus, Denmark
Br J Haematol 131:457-67. 2005.... - Gene expression profiling of Polycomb, Hox and Meis genes in patients with acute myeloid leukaemiaLykke Grubach
Department of Haematology, Aarhus University Hospital, Aarhus, Denmark
Eur J Haematol 81:112-22. 2008..01). In conclusion, our data suggest that the role of PcG and PcG-regulated genes in leukaemogenesis varies between, as well as within karyotypic subgroups... - Relapse prediction in acute myeloid leukaemia patients in complete remission using WT1 as a molecular marker: development of a mathematical model to predict time from molecular to clinical relapse and define optimal sampling intervalsHans Beier Ommen
The Laboratory of Immunohematology, Department of Haematology, Arhus University Hospital, Arhus, Denmark
Br J Haematol 141:782-91. 2008..In conclusion, CR1 WT1 expression is an independent prognostic factor in AML. According to our model, BM is superior for relapse prediction, but PB samples are useful when shorter sampling intervals are possible... - Promoter hypermethylation of p15INK4B, HIC1, CDH1, and ER is frequent in myelodysplastic syndrome and predicts poor prognosis in early-stage patientsAnni Aggerholm
Department of Hematology, Aarhus University Hospital, Aarhus, Denmark
Eur J Haematol 76:23-32. 2006..05). These data suggest that hypermethylation of p15INK4B, HIC1, CDH1, and ER contribute to the development and outcome of MDS... - Persistent altered fusion transcript splicing identifies RUNX1-RUNX1T1+ AML patients likely to relapseHans B Ommen
Laboratory of Immunohematology, Department of Hematology, Aarhus University Hospital, Arhus, Denmark
Eur J Haematol 84:128-32. 2010..In conclusion, alternative splicing seems to be part of the leukemogenic process in the majority of RUNX1-RUNX1T1-positive AML patients, and splice variant kinetics under cytoreduction may be a predictor for patients prone to relapse... - Promoter hypermethylation of the retinoic acid receptor beta2 gene is frequent in acute myeloid leukaemia and associated with the presence of CBFbeta-MYH11 fusion transcriptsAnita Rethmeier
Department of Haematology, Aarhus University Hospital, Aarhus, Denmark
Br J Haematol 133:276-83. 2006..01). Our data suggest that RARbeta2 promoter methylation is frequent in AML and may co-operate with the expression of CBF-MYH11 fusion transcripts in leukaemogenesis... - Prospective application of a multiplex reverse transcription-polymerase chain reaction assay for the detection of balanced translocations in leukaemia: a single-laboratory study of 390 paediatric and adult patientsLene Hyldahl Olesen
Department of Haematology, Aarhus University Hospital, Aarhus, Denmark
Br J Haematol 127:59-66. 2004.... - Minimal residual core binding factor AMLs by real time quantitative PCR--initial response to chemotherapy predicts event free survival and close monitoring of peripheral blood unravels the kinetics of relapseJesper Stentoft
Department of Hematology, Aarhus University Hospital, Tage Hansens Gade 2, DK 8000 Aarhus C, Denmark
Leuk Res 30:389-95. 2006..This direct comparison between the two subgroups of CBF AMLs delineates clear biological differences and corroborates the value of RQ-PCR... - A patient with a 20-year lag phase between JAK2-V617F+ myeloproliferation and NPM1-mutated AML arguing against a common origin of diseaseAnne Stidsholt Roug
Department of Hematology, Aarhus University Hospital, Tage Hansens Gade 2, Aarhus C, Denmark
Eur J Haematol 87:461-3. 2011..The 20-yr lag phase between the polycythemia vera and the AML adds indirect evidence to the growing realization that the leukemic transformation in patients with MPN occurs from in a JAK2 wild-type stem cell... - Genetic and epigenetic similarities and differences between childhood and adult AMLCaroline Juhl-Christensen
Department of Hematology, Aarhus University Hospital, Skejby, Denmark
Pediatr Blood Cancer 58:525-31. 2012..We addressed the combined consequences of promoter hypermethylation of p15, CDH1, ER, MDR1, and RARB2 and mutation of NPM1, CEBPA, FLT3, and WT1 in a Danish cohort of 70 pediatric and 383 adult AML patients... - A real-time quantitative RT-PCR assay for monitoring DEK-CAN fusion transcripts arising from translocation t(6;9) in acute myeloid leukemiaMette Østergaard
Department of Hematology, Aarhus University Hospital, Tage Hansens Gade 2, 8000 C, Denmark
Leuk Res 28:1213-5. 2004..This assay should enable the widespread longitudinal minimal residual disease (MRD) follow-up in this rare subgroup of AML patients, thus adding to our knowledge of its course... - A highly sensitive and specific qPCR assay for quantification of the biomarker SOX11 in mantle cell lymphomaKristiane Hornung Hamborg
Laboratory of Immunohaematology, Aarhus University Hospital, Denmark
Eur J Haematol 89:385-94. 2012..We hypothesised that SOX11 could be a robust molecular biomarker for MCL... - Expression of the hMICL in acute myeloid leukemia-a highly reliable disease marker at diagnosis and during follow-upHanne Ø Larsen
Department of Hematology, The Laboratory of Immunohematology, Aarhus University Hospital, Aarhus, Denmark
Cytometry B Clin Cytom 82:3-8. 2012..Based on data from stem cell research, we hypothesized that the human inhibitory C-type lectin like receptor (hMICL) might represent a novel diagnostic and prognostic vehicle in a standard flow cytometry (FCM) setting... - Delineation and molecular characterization of acute myeloid leukemia patients with coduplication of FLT3 and MLLLene Hyldahl Olesen
Department of Hematology, Aarhus University Hospital, Denmark
Eur J Haematol 75:185-92. 2005..More patients are needed to determine whether coduplication has independent clinical implications compared to patients with single ITD/PTD... - Multiplex PCR for the detection of BCL-1/IGH and BCL-2/IGH gene rearrangements--clinical validation in a prospective study of blood and bone marrow in 258 patients with or suspected of non-Hodgkin's lymphomaCharlotte G Nyvold
Department of Haematology, Aarhus University Hospital, Denmark
Acta Oncol 46:21-30. 2007..We conclude that this multiplex approach allows for easy and sensitive molecular determination of molecular lesions in NHL, which have diagnostic and prognostic importance... - Capillary gel electrophoresis: a simple method for identification of mutations and polymorphisms in the CEBPA gene in patients with acute myeloid leukaemiaCaroline Juhl-Christensen
Laboratory of Immunohematology, Department of Hematology, Aarhus University Hospital, Aarhus, Denmark
Eur J Haematol 81:273-80. 2008..Eight percentage of all AML patients harbour at least one mutation in this gene, increasing up to 15% in the group, where standard karyotypic analysis do not reveal chromosomal aberrations... - Polymorphisms in the genes ERCC2, XRCC3 and CD3EAP influence treatment outcome in multiple myeloma patients undergoing autologous bone marrow transplantationAnnette Vangsted
Department of Haematology, Herlev University Hospital of Copenhagen, DK 2730 Herlev, Denmark
Int J Cancer 120:1036-45. 2007..0002). This indicates that suboptimal repair of both DNA mechanisms favors prolonged TTF and that polymorphism in ERCC2, XRCC3 and CD3EAP predicts the outcome for patients treated with autologous stem cell transplantation... - Nonviral transfection of leukemic primary cells and cells lines by siRNA-a direct comparison between Nucleofection and Accell deliveryHanne Østergård Larsen
Department of Hematology, Aarhus University Hospital, Denmark
Exp Hematol 39:1081-9. 2011..Our results reveal that Accell delivery is suitable for nonviral transfection of cells in suspension, including primary leukemic cells. These data should provide a platform for further studies of genes involved in early leukemogenesis... - Prediction of target CD34 positive cells following leukopheresis in children with neuroblastomaHenrik Schroeder
Department of Pediatrics, University Hospital of Aarhus, Aarhus, Denmark
Pediatr Blood Cancer 46:786-92. 2006..In children it is important to be able to ascertain when to start the leukopheresis in order to keep the number of procedures to a minimum... - Increased expression of CD69 on T cells as an early immune marker for human cytomegalovirus reactivation in chronic lymphocytic leukemia patientsCharlotte C Petersen
Department of Medical Microbiology and Immunology, Aarhus University, Aarhus, Denmark
Viral Immunol 24:165-9. 2011..Moreover, a CD4(+):CD8(+) ratio lower than 0.6 may indicate a trend toward an increased risk for viral reactivation. In conclusion, an increase in CD69 expression is a promising candidate as an early predictor of HCMV reactivation... - Recurrent genomic imbalances in B-cell splenic marginal-zone lymphoma revealed by comparative genomic hybridizationClaus L Andersen
Department of Hematology, Laboratory of Cancer Cytogenetics, Arhus Sygehus, Tage Hansens Gade 2, DK-8000 Arhus C, Denmark
Cancer Genet Cytogenet 156:122-8. 2005..Our data suggest that SMZL constitute a genetically heterogeneous disease where gain of 3q25 and loss of 7q31 are the most likely imbalances to be involved in the pathogenesis of the disease... - WT1 gene expression: an excellent tool for monitoring minimal residual disease in 70% of acute myeloid leukaemia patients - results from a single-centre studyMette Østergaard
Department of Haematology, Aarhus University Hospital, Aarhus, Denmark
Br J Haematol 125:590-600. 2004..We conclude that WT1 expression by RQ-PCR may be employed as a tool to detect MRD in the majority of fusion transcript-negative AML patients... - Changing picture of cellular drug resistance in human leukemiaJan Maxwell Nørgaard
Department of Hematology, Aarhus University Hospital, DK 8000 Aarhus C, Denmark
Crit Rev Oncol Hematol 50:39-49. 2004..To what extent drug resistance towards this novel compound (and its successors) will follow patterns of drug resistance that are already known or entirely new mechanisms of drug resistance is yet to be seen...