Peter Dedecker

Summary

Publications

  1. doi request reprint Fluorescent proteins: shine on, you crazy diamond
    Peter Dedecker
    Department of Chemistry, University of Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Am Chem Soc 135:2387-402. 2013
  2. pmc Localizer: fast, accurate, open-source, and modular software package for superresolution microscopy
    Peter Dedecker
    The Johns Hopkins University School of Medicine, Department of Pharmacology and Molecular Sciences, 725 N Wolfe Street, Baltimore, Mayland 21205, USA
    J Biomed Opt 17:126008. 2012
  3. pmc Widely accessible method for superresolution fluorescence imaging of living systems
    Peter Dedecker
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 109:10909-14. 2012
  4. ncbi request reprint A stroboscopic approach for fast photoactivation-localization microscopy with Dronpa mutants
    Cristina Flors
    Department of Chemistry and Institute for Nanoscale Physics and Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Am Chem Soc 129:13970-7. 2007
  5. ncbi request reprint Subdiffraction imaging through the selective donut-mode depletion of thermally stable photoswitchable fluorophores: numerical analysis and application to the fluorescent protein Dronpa
    Peter Dedecker
    Department of Chemistry and INPAC, Katholieke Universiteit Leuven, Celestijnenlaan 200 F, 3001 Heverlee, Belgium
    J Am Chem Soc 129:16132-41. 2007
  6. doi request reprint Rational design of photoconvertible and biphotochromic fluorescent proteins for advanced microscopy applications
    Virgile Adam
    Laboratory of Photochemistry and Spectroscopy, Department of Chemistry, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium
    Chem Biol 18:1241-51. 2011
  7. doi request reprint Structural basis for the influence of a single mutation K145N on the oligomerization and photoswitching rate of Dronpa
    Ngan Nguyen Bich
    Biomolecular Architecture, Department of Chemistry, KU Leuven, Celestijnenlaan 200F, Leuven Heverlee, Belgium
    Acta Crystallogr D Biol Crystallogr 68:1653-9. 2012
  8. pmc Fast and reversible photoswitching of the fluorescent protein dronpa as evidenced by fluorescence correlation spectroscopy
    Peter Dedecker
    Laboratory of Photochemistry and Spectroscopy, Department of Chemistry, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium
    Biophys J 91:L45-7. 2006
  9. ncbi request reprint Photo-induced protonation/deprotonation in the GFP-like fluorescent protein Dronpa: mechanism responsible for the reversible photoswitching
    Satoshi Habuchi
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    Photochem Photobiol Sci 5:567-76. 2006
  10. pmc Dark states in monomeric red fluorescent proteins studied by fluorescence correlation and single molecule spectroscopy
    Jelle Hendrix
    Laboratory of Biomolecular Dynamics, Department of Chemistry, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium
    Biophys J 94:4103-13. 2008

Collaborators

Detail Information

Publications17

  1. doi request reprint Fluorescent proteins: shine on, you crazy diamond
    Peter Dedecker
    Department of Chemistry, University of Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Am Chem Soc 135:2387-402. 2013
    ..We pay particular attention to emerging or future research opportunities and unsolved questions...
  2. pmc Localizer: fast, accurate, open-source, and modular software package for superresolution microscopy
    Peter Dedecker
    The Johns Hopkins University School of Medicine, Department of Pharmacology and Molecular Sciences, 725 N Wolfe Street, Baltimore, Mayland 21205, USA
    J Biomed Opt 17:126008. 2012
    ..By dramatically improving the analysis performance and ensuring the easy addition of current and future enhancements, Localizer strongly improves the usability of superresolution imaging in a variety of biomedical studies...
  3. pmc Widely accessible method for superresolution fluorescence imaging of living systems
    Peter Dedecker
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 109:10909-14. 2012
    ..Furthermore, both 3D and multicolor imaging are readily achievable. Because of its ease of use and high performance, we anticipate that pcSOFI will prove an attractive approach for superresolution imaging...
  4. ncbi request reprint A stroboscopic approach for fast photoactivation-localization microscopy with Dronpa mutants
    Cristina Flors
    Department of Chemistry and Institute for Nanoscale Physics and Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Am Chem Soc 129:13970-7. 2007
    ..We provide a new approach to achieve fast PALM by introducing simultaneous two-color stroboscopic illumination...
  5. ncbi request reprint Subdiffraction imaging through the selective donut-mode depletion of thermally stable photoswitchable fluorophores: numerical analysis and application to the fluorescent protein Dronpa
    Peter Dedecker
    Department of Chemistry and INPAC, Katholieke Universiteit Leuven, Celestijnenlaan 200 F, 3001 Heverlee, Belgium
    J Am Chem Soc 129:16132-41. 2007
    ..We experimentally demonstrate the possibility of obtaining increased resolution by making use of the efficient and thermally stable Dronpa photoswitching, using equipment that is commonly available...
  6. doi request reprint Rational design of photoconvertible and biphotochromic fluorescent proteins for advanced microscopy applications
    Virgile Adam
    Laboratory of Photochemistry and Spectroscopy, Department of Chemistry, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium
    Chem Biol 18:1241-51. 2011
    ..We present in particular NijiFP, a promising new fluorescent protein with photoconvertible and biphotochromic properties that make it ideal for advanced fluorescence-based imaging applications...
  7. doi request reprint Structural basis for the influence of a single mutation K145N on the oligomerization and photoswitching rate of Dronpa
    Ngan Nguyen Bich
    Biomolecular Architecture, Department of Chemistry, KU Leuven, Celestijnenlaan 200F, Leuven Heverlee, Belgium
    Acta Crystallogr D Biol Crystallogr 68:1653-9. 2012
    ..It is proposed that the slower off-switching rate in PDM1-4 is caused by a decrease in the potential flexibility of certain β-strands caused by oligomerization along the AC interface...
  8. pmc Fast and reversible photoswitching of the fluorescent protein dronpa as evidenced by fluorescence correlation spectroscopy
    Peter Dedecker
    Laboratory of Photochemistry and Spectroscopy, Department of Chemistry, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium
    Biophys J 91:L45-7. 2006
    ....
  9. ncbi request reprint Photo-induced protonation/deprotonation in the GFP-like fluorescent protein Dronpa: mechanism responsible for the reversible photoswitching
    Satoshi Habuchi
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    Photochem Photobiol Sci 5:567-76. 2006
    ..Our results demonstrate that Dronpa holds its outstanding photoswitching properties, based on a photo-induced protonation/deprotonation process, even at the single-molecule level...
  10. pmc Dark states in monomeric red fluorescent proteins studied by fluorescence correlation and single molecule spectroscopy
    Jelle Hendrix
    Laboratory of Biomolecular Dynamics, Department of Chemistry, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium
    Biophys J 94:4103-13. 2008
    ..We apply dual-color fluorescence correlation spectroscopy inside live cells to prove that this flickering can seriously hamper cellular measurements if the timescales of the flickering and diffusion are not well separated...
  11. doi request reprint Green-to-Red Photoconvertible Dronpa Mutant for Multimodal Super-resolution Fluorescence Microscopy
    Benjamien Moeyaert
    Department of Chemistry, KU Leuven, Celestijnenlaan 200F, bus 2404, 3001 Heverlee, Belgium
    ACS Nano 8:1664-73. 2014
    ..We herein show that, despite its tetrameric nature, pcDronpa2 allows for multimodal subdiffraction imaging by sequentially imaging a given sample using both super-resolution fluctuation imaging and localization microscopy. ..
  12. doi request reprint Spectroscopic characterization of Venus at the single molecule level
    Charlotte C David
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001, Heverlee, Belgium
    Photochem Photobiol Sci 11:358-63. 2012
    ..Such long off-times can complicate the analysis of single molecule counting experiments or single-molecule FRET experiments...
  13. ncbi request reprint 3D nanoscopy: bringing biological nanostructures into sharp focus
    Peter Dedecker
    Department of Chemistry and INPAC, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    Angew Chem Int Ed Engl 46:8330-2. 2007
  14. doi request reprint Excited state dynamics of photoswitchable fluorescent protein Padron
    Eduard Fron
    Molecular Imaging and Photonics, Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    J Phys Chem B 117:16422-7. 2013
    ..Consequently, upon excitation of the formed anionic species a trans-cis isomerization of the chromophore was found to occur with a time constant as fast as 5.2 ps switching the chromophore quantitatively to the bright (anionic) state. ..
  15. doi request reprint Spectroscopic rationale for efficient stimulated-emission depletion microscopy fluorophores
    Jun ichi Hotta
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, B 3001 Heverlee, Belgium
    J Am Chem Soc 132:5021-3. 2010
    ..Thus, assessment of the excited-state absorption band provides a rational means of dye selection and determination of the optimal wavelength for STED...
  16. doi request reprint Synthesis, spectroscopy, crystal structure, electrochemistry, and quantum chemical and molecular dynamics calculations of a 3-anilino difluoroboron dipyrromethene dye
    Wenwu Qin
    Department of Chemistry, Katholieke Universiteit Leuven, Leuven, Belgium
    J Phys Chem A 113:439-47. 2009
    ..7) x 10(8) s(-1) range and do not vary much between the solvents studied. X-ray diffraction analysis shows that the BODIPY core is planar. Molecular dynamics simulations show that there is no clear indication for aggregates in solution...
  17. pmc Reversible single-molecule photoswitching in the GFP-like fluorescent protein Dronpa
    Satoshi Habuchi
    Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
    Proc Natl Acad Sci U S A 102:9511-6. 2005
    ..The fast response of Dronpa to light holds great promise for following fast diffusion or transport of signaling molecules in live cells...