Growth regulation and enhancement in tilapia: basic research findings and their applicationsJ de la Fuente
Mammalian Cell Genetics Division, Centro de Ingenieria Genetica y Biotecnologia, Havana, Cuba
Genet Anal 15:85-90. 1999
..Our experiments produced new scientific results on growth control in tilapia. These results were used to develop a new aquacultured line with improved growth performance. Many of these results are probably applicable to other teleosts...
- Prevalence of Coxiella burnetti infection in wild and farmed ungulates
Francisco Ruiz-Fons
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, 13071 Ciudad Real, Spain
Vet Microbiol 126:282-6. 2008
..These results show that C. burnetii circulates in wild ungulates in Spain and suggest that they can act as pathogen reservoirs for both domestic animals and humans...
Identification and characterization of Rhipicephalus (Boophilus) microplus candidate protective antigens for the control of cattle tick infestationsConsuelo Almazan
Facultad de Medicina Veterinaria y Zootecnia, Universidad Autonoma de Tamaulipas, Km 5 carretera Victoria Mante, CP 87000, Victoria, Tamaulipas, Mexico
Parasitol Res 106:471-9. 2010
..However, vaccination trials are necessary to evaluate the effect of recombinant antigens in the control of tick infestations, a process that requires efficient recombinant protein production and formulation systems...
- Subolesin expression in response to pathogen infection in ticks
Zorica Zivkovic
Department of Infectious Diseases and Immunology, Utrecht Centre for Tick Borne Diseases, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584CL Utrecht, The Netherlands
BMC Immunol 11:7. 2010
..Finally, the effect of subolesin knockdown by RNA interference (RNAi) on tick infection was analyzed in Dermacentor variabilis males exposed to various pathogens by capillary feeding (CF)...
- Application of highly sensitive saturation labeling to the analysis of differential protein expression in infected ticks from limited samples
Margarita Villar
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, 13005 Ciudad Real, Spain
Proteome Sci 8:43. 2010
..To our knowledge, this is the first report of the application of DIGE saturation labeling to study tick proteins...
- Gene expression profile suggests that pigs (Sus scrofa) are susceptible to Anaplasma phagocytophilum but control infection
Ruth C Galindo
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, Ciudad Real 13005, Spain
Parasit Vectors 5:181. 2012
..phagocytophilum trying to identify mechanisms that help to explain low infection prevalence in this species...
- Experimental transmission of Anaplasma marginale by male Dermacentor reticulatus
Zorica Zivkovic
Utrecht Centre for Tick borne Diseases UCTD, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584CL, Utrecht, The Netherlands
BMC Vet Res 3:32. 2007
..as a vector of A. marginale in the United States, we tested the vector competency of D. reticulatus for A. marginale...
- Functional genomics of the horn fly, Haematobia irritans (Linnaeus, 1758)
Lorena Torres
Facultad de Medicina Veterinaria y Zootecnia, Universidad Autonoma de Tamaulipas, km, 5 carretera Victoria Mante, CP 87000 Ciudad Victoria, Tamaulipas, Mexico
BMC Genomics 12:105. 2011
..The aim of this study was to conduct a functional genomics study in female horn flies using Expressed Sequence Tags (EST) analysis and RNA interference (RNAi)...
- Differential expression of genes in salivary glands of male Rhipicephalus (Boophilus)microplus in response to infection with Anaplasma marginale
Zorica Zivkovic
Department of Infectious Diseases and Immunology, Utrecht Centre for Tick borne Diseases UCTD, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584CL Utrecht, The Netherlands
BMC Genomics 11:186. 2010
..microplus male salivary glands in response to A. marginale infection. Additionally, a R. microplus-derived cell line, BME26, was used for the first time to also study tick cell gene expression in response to A. marginale infection...
- Silencing of genes involved in Anaplasma marginale-tick interactions affects the pathogen developmental cycle in Dermacentor variabilis
Katherine M Kocan
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
BMC Dev Biol 9:42. 2009
..marginale developmental cycle. These four genes encoded for putative glutathione S-transferase (GST), salivary selenoprotein M (SelM), H+ transporting lysosomal vacuolar proton pump (vATPase) and subolesin...
- Phylogeographic analysis reveals association of tick-borne pathogen, Anaplasma marginale, MSP1a sequences with ecological traits affecting tick vector performance
Agustin Estrada-Pena
Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain
BMC Biol 7:57. 2009
..marginale-tick co-evolution, these studies have not provided phylogeographic information on a global scale because of the high level of MSP1a genetic diversity among geographic strains...
- The tick protective antigen, 4D8, is a conserved protein involved in modulation of tick blood ingestion and reproduction
Jose De La Fuente
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
Vaccine 24:4082-95. 2006
....
- Synergistic effect of silencing the expression of tick protective antigens 4D8 and Rs86 in Rhipicephalus sanguineus by RNA interference
Jose De La Fuente
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078 2007, USA
Parasitol Res 99:108-13. 2006
..These results demonstrated the synergistic effect of the silencing expression of two tick protective genes. Inclusion of multiple tick protective antigens may, therefore, enhance the efficacy of tick vaccines...
- Potential vertebrate reservoir hosts and invertebrate vectors of Anaplasma marginale and A. phagocytophilum in central Spain
Jose De La Fuente
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ciudad Real, Spain
Vector Borne Zoonotic Dis 5:390-401. 2005
..The increasing contact between wildlife, domestic animals, and human populations increases the risk of outbreaks of human and bovine anaplasmosis, and the difficulty of implementing surveillance and control measures...
- Genetic diversity of anaplasma species major surface proteins and implications for anaplasmosis serodiagnosis and vaccine development
Jose De La Fuente
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078 2007, USA
Anim Health Res Rev 6:75-89. 2005
..Information concerning the genetic diversity of Anaplasma spp. MSPs will likely influence the development of serodiagnostic assays and vaccine strategies for the control of anaplasmosis...
- Functional genomic studies of tick cells in response to infection with the cattle pathogen, Anaplasma marginale
Jose De La Fuente
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
Genomics 90:712-22. 2007
..The results of these studies demonstrated that a molecular mechanism occurs by which tick cell gene expression mediates the A. marginale developmental cycle and trafficking through ticks...
- Genetic diversity of Anaplasma marginale strains from cattle farms in the province of Palermo, Sicily
J de la Fuente
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
J Vet Med B Infect Dis Vet Public Health 52:226-9. 2005
..These results suggest that development of farm husbandry systems and vaccines for genetically heterogeneous populations of A. marginale are needed for control of anaplasmosis in this region of Sicily...
- Gene expression profiling of human promyelocytic cells in response to infection with Anaplasma phagocytophilum
Jose De La Fuente
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
Cell Microbiol 7:549-59. 2005
..phagocytophilum-infected HL-60 cells, thus expanding in a global manner the existing information on the response of mammalian cells to A. phagocytophilum infection...
- RNA interference screening in ticks for identification of protective antigens
Jose De La Fuente
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
Parasitol Res 96:137-41. 2005
....
- Serologic and molecular characterization of Anaplasma species infection in farm animals and ticks from Sicily
Jose De La Fuente
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, 250 McElory Hall, Stillwater, OK 74078, USA
Vet Parasitol 133:357-62. 2005
..Definition of the diversity of Anaplasma spp. in Sicily reported, herein is fundamental to development of control strategies for A. marginale, A. ovis and A. phagocytophilum in Sicily...
- Autocidal control of ticks by silencing of a single gene by RNA interference
Jose De La Fuente
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
Biochem Biophys Res Commun 344:332-8. 2006
..Conservation of subolesin among tick species suggests that SAT may be useful for control of many medically and economically important tick species...
- Reduction of tick infections with Anaplasma marginale and A. phagocytophilum by targeting the tick protective antigen subolesin
Jose De La Fuente
Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, 250 McElroy Hall, Stillwater, OK 74078, USA
Parasitol Res 100:85-91. 2006
..These results suggest that subolesin appears to be a candidate vaccine antigen that may contribute to control of multiple tick species and the reduction of tick-borne pathogens...
- Genetic characterization of Anaplasma ovis strains from bighorn sheep in Montana
Jose De La Fuente
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, USA
J Wildl Dis 42:381-5. 2006
..marginale in cattle herds in the western United States and worldwide. These results demonstrated that bighorn sheep may be a wildlife reservoir of A. ovis in Montana...
Host expression of methylmalonyl-CoA mutase and tuberculosis: a missing link?Jose De La Fuente
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, 13005 Ciudad Real, Spain
Med Hypotheses 76:361-4. 2011
..If the hypothesis is proven true, these results have important implications for the prevention and treatment of bTB in humans and for the eradication of bTB in wildlife reservoir hosts...
- Identification of protective antigens by RNA interference for control of the lone star tick, Amblyomma americanum
Jose De La Fuente
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
Vaccine 28:1786-95. 2010
....
- Functional genomics and evolution of tick-Anaplasma interactions and vaccine development
Jose De La Fuente
Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK 74078, USA
Vet Parasitol 167:175-86. 2010
....
- A ten-year review of commercial vaccine performance for control of tick infestations on cattle
Jose De La Fuente
Center for Veterinary Health Sciences, Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK 74078 2007, USA
Anim Health Res Rev 8:23-8. 2007
....
- Evidence of Anaplasma infections in European roe deer (Capreolus capreolus) from southern Spain
Jose De La Fuente
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, 13071 Ciudad Real, Spain
Res Vet Sci 84:382-6. 2008
..ovis and A. phagocytophilum in Spain and suggest that this species may be involved in the natural cycle of these pathogens in this region, thus acting as potential reservoir for transmission to domestic and wild animals...
- Anaplasmosis: focusing on host-vector-pathogen interactions for vaccine development
Jose De La Fuente
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
Ann N Y Acad Sci 1078:416-23. 2006
..The screening for tick-protective antigens resulted in vaccine candidates reducing tick infestation, molting, and oviposition and affecting Anaplasma infection levels in ticks...
- Analysis of world strains of Anaplasma marginale using major surface protein 1a repeat sequences
Jose De La Fuente
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078 2007, USA
Vet Microbiol 119:382-90. 2007
..marginale strains from various geographic locations worldwide. These results contribute to the understanding of the genetic diversity and evolution of A. marginale and tick-pathogen interactions...
- Differential expression of the tick protective antigen subolesin in anaplasma marginale- and A. phagocytophilum-infected host cells
Jose De La Fuente
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, Oklahoma, USA
Ann N Y Acad Sci 1149:27-35. 2008
..The results reported herein further support the role of subolesin in Anaplasma-host interactions and suggest a putative role of subolesin in vaccines for the control of pathogen infection/multiplication in ticks...
- Sequence analysis of the msp4 gene of Anaplasma ovis strains
Jose De La Fuente
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
Vet Microbiol 119:375-81. 2007
..The results reported herein further confirm that A. ovis infection occurs in natural wild ruminant populations in Western United States and that bighorn sheep and mule deer may serve as wildlife reservoirs of A. ovis...
- Sequence analysis of the msp4 gene of Anaplasma phagocytophilum strains
Jose De La Fuente
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA
J Clin Microbiol 43:1309-17. 2005
..These results suggest that A. phagocytophilum strains from ruminants could share some common characteristics, including reservoirs and pathogenicity, which may be different from strains that infect humans...
- Characterization of genetic diversity in Dermacentor andersoni (Acari: Ixodidae) with body size and weight polymorphism
Jose De La Fuente
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA
Exp Parasitol 109:16-26. 2005
..The results reported herein show that B ticks have a better reproductive success than S ticks and suggest partial reproductive isolation of S ticks...
- Anaplasma marginale msp1alpha genotypes evolved under positive selection pressure but are not markers for geographic isolates
Jose De La Fuente
Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, Oklahoma 74708, USA
J Clin Microbiol 41:1609-16. 2003
..marginale within Oklahoma could be explained by cattle movement and the maintenance of different genotypes by independent transmission events...
- Characterization of Anaplasma marginale isolated from North American bison
Jose De La Fuente
Department of Veterinary Pathobiology, College of Veterinary Medicine, Stillwater, Oklahoma 74078, USA
Appl Environ Microbiol 69:5001-5. 2003
..marginale bison isolate for cattle. The results of this study showed that these A. marginale isolates obtained from bison were similar to ones from naturally infected cattle...
- Immunological control of ticks through vaccination with Boophilus microplus gut antigens
J de la Fuente
Mammalian Cell Genetics Division, Centro de Ingenieria Genetica y Biotecnologia, P O Box 6162, Havana, Cuba
Ann N Y Acad Sci 916:617-21. 2000
..microplus strains from different geographical areas. These results clearly demonstrate the advantage and possibilities for the immunological control of ticks...
- Differential adhesion of major surface proteins 1a and 1b of the ehrlichial cattle pathogen Anaplasma marginale to bovine erythrocytes and tick cells
J de la Fuente
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA
Int J Parasitol 31:145-53. 2001
..marginale adhesin for both bovine erythrocytes and tick cells while MSP1b is an adhesin only for bovine erythrocytes. The role of the MSP1 complex, therefore, appears to vary among vertebrate and invertebrate hosts...
- Molecular phylogeny and biogeography of North American isolates of Anaplasma marginale (Rickettsiaceae: Ehrlichieae)
J de la Fuente
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA
Vet Parasitol 97:65-76. 2001
..variabilis. These phylogenetic analyses provide information that may be important to consider when developing control strategies for anaplasmosis in the United States...
- Major surface protein 1a effects tick infection and transmission of Anaplasma marginale
J de la Fuente
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA
Int J Parasitol 31:1705-14. 2001
..marginale by Dermacentor spp. ticks. The mechanism of tick infection and transmission of A. marginale is important in formulating control strategies and development of improved vaccines for anaplasmosis...
- Evolution and function of tandem repeats in the major surface protein 1a of the ehrlichial pathogen Anaplasma marginale
J de la Fuente
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078, USA
Anim Health Res Rev 2:163-73. 2001
..These repeated sequences include markers for tick transmissibility that may be important in the identification of ehrlichial pathogens because they may influence control strategies and the design of subunit vaccines...
- Infection of tick cells and bovine erythrocytes with one genotype of the intracellular ehrlichia Anaplasma marginale excludes infection with other genotypes
Jose De La Fuente
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, Oklahoma 74078, USA
Clin Diagn Lab Immunol 9:658-68. 2002
..marginale in bovine erythrocytes and tick cells, resulting in the establishment of only one genotype, and appears to be the first report of infection exclusion for Anaplasma and Ehrlichia species...
- Infection exclusion of the rickettsial pathogen anaplasma marginale in the tick vector Dermacentor variabilis
Jose De La Fuente
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, Oklahoma 74078, USA
Clin Diagn Lab Immunol 10:182-4. 2003
..These studies demonstrate that infection exclusion of A. marginale genotypes also occurs in naturally infected ticks, as well as in cattle and cultured tick cells, and results in establishment of only one genotype per tick...
- Phylogeography of New World isolates of Anaplasma marginale based on major surface protein sequences
Jose De La Fuente
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, 250 McElroy Hall, Stillwater, OK 74078, USA
Vet Microbiol 88:275-85. 2002
..marginale isolates. In contrast MSP1a sequences appeared to be rapidly evolving and these sequences may provide phylogeographic information only when numerous isolate MSP1a sequences are analyzed from a geographic area...
- Characterization of the functional domain of major surface protein 1a involved in adhesion of the rickettsia Anaplasma marginale to host cells
Jose De La Fuente
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, 250 McElroy Hall, 74078 2007, Stillwater, OK, USA
Vet Microbiol 91:265-83. 2003
..These studies demonstrated that the tandemly repeated region of the protein mediates the adhesive function of MSP1a...
- Molecular analysis of Boophilus spp. (Acari: Ixodidae) tick strains
J Fuente
Division of Mammalian Cell Genetics, Centro de Ingeniería Genética y Biotecnología P O Box 6162, Havana, Cuba
Vet Parasitol 92:209-22. 2000
..microplus. These results support at the molecular level the existing differences between B. microplus strains and suggest tools to characterize these populations...
- Vaccination against ticks (Boophilus spp.): the experience with the Bm86-based vaccine Gavac
J de la Fuente
Division of Mammalian Cell Genetics, Centro de Ingenieria Genetica y Biotecnologia, Havana, Cuba
Genet Anal 15:143-8. 1999
..4 dollars animal(-1) year (-1). These results clearly demonstrate the advantage of vaccination and support the application of Gavac for the control of Boophilus spp. infestations...
- Vaccination of cattle with Anaplasma marginale derived from tick cell culture and bovine erythrocytes followed by challenge-exposure with infected ticks
Jose De La Fuente
Department of Veterinary Pathobiology, College of Veterinary Medicine, 250 McElory Hall, Oklahoma State University, Stillwater, OK 74078 2007, USA
Vet Microbiol 89:239-251. 2002
..The cell culture-derived A. marginale shows promise for use as antigen in development of a new killed vaccine for anaplasmosis...
- The fossil record and the origin of ticks (Acari: Parasitiformes: Ixodida)
Jose De La Fuente
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, 250 McElroy Hall, Stillwater, OK 74078, USA
Exp Appl Acarol 29:331-44. 2003
....
- Anaplasma infection in free-ranging Iberian red deer in the region of Castilla-La Mancha, Spain
Jose De La Fuente
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, 13005 Ciudad Real, Spain
Vet Microbiol 100:163-73. 2004
..marginatum and Rhipicephalus bursa were identified as potential biological vectors for A. marginale in this region and may effect transmission of A. marginale between deer and cattle populations...
- Advances in the identification and characterization of protective antigens for recombinant vaccines against tick infestations
Jose De La Fuente
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA
Expert Rev Vaccines 2:583-93. 2003
..Design of future tick vaccines should target multiple tick species, as well as interfere with the transmission of pathogens...
- Genetic diversity and molecular phylogeny of Anaplasma marginale isolates from Minas Gerais, Brazil
Jose De La Fuente
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, 250 McElroy Hall, Stillwater, OK 74078, USA
Vet Parasitol 121:307-16. 2004
..marginale isolates, while MSP1a was found not to be a useful marker for phylogeographic characterization of A. marginale isolates...
- Adhesion of outer membrane proteins containing tandem repeats of Anaplasma and Ehrlichia species (Rickettsiales: Anaplasmataceae) to tick cells
Jose De La Fuente
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA
Vet Microbiol 98:313-22. 2004
..The results reported herein provide new information on the role of E. ruminantium "mucin" as an adhesin for tick cells and also suggest a role of glycans in adhesin molecules...
- Vaccination with BM86, subolesin and akirin protective antigens for the control of tick infestations in white tailed deer and red deer
Diana Carreón
Instituto de Ecología Aplicada, Universidad Autonoma de Tamaulipas, Avenida División del Golfo 356, Col Libertad, 87019 Ciudad Victoria, Tamaulipas, Mexico
Vaccine 30:273-9. 2012
....
- Antibodies to Anaplasma marginale major surface proteins 1a and 1b inhibit infectivity for cultured tick cells
Edmour F Blouin
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, 250 McElroy Hall, Stillwater, OK 74078 2007, USA
Vet Parasitol 111:247-60. 2003
..marginale for tick cells and may contribute to the continued infectivity of the pathogen for ticks...
- Differential expression of inflammatory and immune response genes in mesenteric lymph nodes of Iberian red deer (Cervus elaphus hispanicus) naturally infected with Mycobacterium bovis
Isabel G Fernandez de Mera
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, 13071 Ciudad Real, Spain
Dev Comp Immunol 32:85-91. 2008
..These results contribute to our basic understanding of the mechanisms of pathogenesis and immunity to natural mycobacterial infections and may have important implications for the control of bTB...
- Characterization of pathogen-specific expression of host immune response genes in Anaplasma and Mycobacterium species infected ruminants
Ruth C Galindo
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, 13005 Ciudad Real, Spain
Comp Immunol Microbiol Infect Dis 33:e133-42. 2010
..However, pathogen and host-specific differences could contribute to disease diagnosis and treatment in ruminants...
- Control of tick infestations in cattle vaccinated with bacterial membranes containing surface-exposed tick protective antigens
Consuelo Almazan
Facultad de Medicina Veterinaria y Zootecnia, Universidad Autonoma de Tamaulipas, Km 5 carretera Victoria Mante, CP 87000 Ciudad Victoria, Tamaulipas, Mexico
Vaccine 30:265-72. 2012
..coli membrane and demonstrates the possibility of using recombinant bacterial membrane fractions in vaccine preparations to protect cattle against tick infestations...
- Control of Rhipicephalus (Boophilus) microplus infestations by the combination of subolesin vaccination and tick autocidal control after subolesin gene knockdown in ticks fed on cattle
Octavio Merino
Facultad de Medicina Veterinaria y Zootecnia, Universidad Autonoma de Tamaulipas, Km 5 carretera Victoria Mante, CP 87000 Ciudad Victoria, Tamaulipas, Mexico
Vaccine 29:2248-54. 2011
..microplus tick infestations, alone or in combination with subolesin vaccination...
- Progress in the control of bovine tuberculosis in Spanish wildlife
Christian Gortazar
IREC CSIC UCLM JCCM, Ronda de Toledo s n, Ciudad Real 13071, Spain
Vet Microbiol 151:170-8. 2011
..Finally, preliminary vaccination and challenge experiments suggested that a single oral BCG vaccination may protect wild boar from infection by a virulent M. bovis field strain...
- Silencing expression of the defensin, varisin, in male Dermacentor variabilis by RNA interference results in reduced Anaplasma marginale infections
Katherine M Kocan
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK, USA
Exp Appl Acarol 46:17-28. 2008
..This appears to be the first report of the silencing of expression of a defensin in ticks by RNAi that resulted in reduced A. marginale infections...
- Recent studies on the characterization of Anaplasma marginale isolated from North American bison
Katherine M Kocan
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078 2007, USA
Ann N Y Acad Sci 1026:114-7. 2004
..the results of this study demonstrated that these A. marginale isolates obtained from bison were similar to ones derived from naturally infected cattle...
- Vaccination with recombinant Boophilus annulatus Bm86 ortholog protein, Ba86, protects cattle against B. annulatus and B. microplus infestations
Mario Canales
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, Ciudad Real, Spain
BMC Biotechnol 9:29. 2009
..Recently, the gene coding for B. annulatus Bm86 ortholog, Ba86, was cloned and the recombinant protein was secreted and purified from the yeast Pichia pastoris...
- Gene expression changes in spleens of the wildlife reservoir species, Eurasian wild boar (Sus scrofa), naturally infected with Brucella suis biovar 2
Ruth C Galindo
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, 13005 Ciudad Real, Spain
J Genet Genomics 37:725-36. 2010
..suis bv. 2, which is important to understand the molecular mechanisms at the host-pathogen interface in the main reservoir species with possible implications in the zoonotic cycle of the pathogen...
- Ixodid ticks parasitizing Iberian red deer (Cervus elaphus hispanicus) and European wild boar (Sus scrofa) from Spain: geographical and temporal distribution
Francisco Ruiz-Fons
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, 13071 Ciudad Real, Spain
Vet Parasitol 140:133-42. 2006
..These results are important for understanding the role of wild ungulates in the maintenance of tick infestations and to improve tick control programmes...
- Comparative genomics and proteomics to study tissue-specific response and function in natural Mycobacterium bovis infections
Victoria Naranjo
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, 13071 Ciudad Real, Spain
Anim Health Res Rev 8:81-8. 2007
..bovis infection and to design effective strategies for the control of bTB in wildlife reservoirs...
- Characterization of three Ixodes scapularis cDNAs protective against tick infestations
Consuelo Almazan
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
Vaccine 23:4403-16. 2005
....
- Targeting arthropod subolesin/akirin for the development of a universal vaccine for control of vector infestations and pathogen transmission
Jose De La Fuente
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, 13005 Ciudad Real, Spain jose
Vet Parasitol 181:17-22. 2011
..However, further research is needed in critical areas towards achieving this goal...
- Conservation and immunogenicity of the mosquito ortholog of the tick-protective antigen, subolesin
Mario Canales
Instituto de Investigación en Recursos Cinegéticos IREC, Ciudad Real, Spain
Parasitol Res 105:97-111. 2009
....
- Targeting the tick protective antigen subolesin reduces vector infestations and pathogen infection by Anaplasma marginale and Babesia bigemina
Octavio Merino
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ciudad Real, Spain
Vaccine 29:8575-9. 2011
..These results suggested that subolesin vaccines could be used for the dual control of tick infestations and pathogen infection, a result that could be relevant for other vectors and vector-borne pathogens...
- Genetic diversity of Anaplasma marginale strains from an outbreak of bovine anaplasmosis in an endemic area
Consuelo Almazan
Facultad de Medicina Veterinaria y Zootecnia, Universidad Autonoma de Tamaulipas, Km 5 carretera Victoria Mante, CP 87000 Victoria, Tamaulipas, Mexico
Vet Parasitol 158:103-9. 2008
..marginale in endemic regions, including the characterization of the genetic diversity of strains that produce outbreaks of bovine anaplasmosis...
- Evidence of the role of tick subolesin in gene expression
Jose De La Fuente
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
BMC Genomics 9:372. 2008
..The objective of this study was to provide evidence for the role of subolesin in gene expression...
- Gene expression profiles of European wild boar naturally infected with Mycobacterium bovis
Ruth C Galindo
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ciudad Real, Spain
Vet Immunol Immunopathol 129:119-25. 2009
..Gene expression studies in naturally infected wildlife reservoirs of bovine tuberculosis are important for functional genomics and vaccine studies to aid in disease control in wildlife...
- Bovine tuberculosis in Doñana Biosphere Reserve: the role of wild ungulates as disease reservoirs in the last Iberian lynx strongholds
Christian Gortazar
IREC National Wildlife Research Institute CSIC UCLM JCCM, Ciudad Real, Spain
PLoS ONE 3:e2776. 2008
..Our results support the need to consider wildlife species for the control of bTB in cattle and strongly suggest that bTB may affect animal welfare and conservation...
- Differential expression of inflammatory and immune response genes in sheep infected with Anaplasma phagocytophilum
Ruth C Galindo
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, 13005 Ciudad Real, Spain
Vet Immunol Immunopathol 126:27-34. 2008
..Collectively, these results expand current information on the mammalian host response to A. phagocytophilum infection...
- Sp110 transcription is induced and required by Anaplasma phagocytophilum for infection of human promyelocytic cells
Jose De La Fuente
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
BMC Infect Dis 7:110. 2007
..In this research, we hypothesized that Sp110 may be involved in the infection of human promyelocytic HL-60 cells with A. phagocytophilum...
- Protective efficacy of bacterial membranes containing surface-exposed BM95 antigenic peptides for the control of cattle tick infestations
Mario Canales
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, 13005 Ciudad Real, Spain
Vaccine 27:7244-8. 2009
..coli and suggests the possibility of using recombinant bacterial membrane fractions for vaccination against cattle tick infestations...
- Tick subolesin is an ortholog of the akirins described in insects and vertebrates
Ruth C Galindo
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, 13005 Ciudad Real, Spain
Dev Comp Immunol 33:612-7. 2009
..These results suggest that these proteins have an important role in host-pathogen interactions...
- Mapping protective epitopes in the tick and mosquito subolesin ortholog proteins
Carlos R Prudencio
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, 13005 Ciudad Real, Spain
Vaccine 28:5398-406. 2010
....
- Differential expression of inflammatory and immune response genes in rams experimentally infected with a rough virulent strain of Brucella ovis
Ruth C Galindo
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, 13005 Ciudad Real, Spain
Vet Immunol Immunopathol 127:295-303. 2009
..ovis-infected rams. The characterization of the genes and their expression profiles in response to B. ovis infection further contributes to our understanding of the molecular mechanisms of infection and the pathogenesis of brucellosis...
- Lesions associated with Mycobacterium tuberculosis complex infection in the European wild boar
María Paz Martín Hernando
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, 13071 Ciudad Real, Spain
Tuberculosis (Edinb) 87:360-7. 2007
..Further studies on the routes of excretion as well as the effect of altering management methods would be of interest to confirm the role of wild boar in TB epidemiology in Spain...
- Expression of perilipin in human promyelocytic cells in response to Anaplasma phagocytophilum infection results in modified lipid metabolism
Raúl Manzano-Román
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
J Med Microbiol 57:159-63. 2008
..The results of these studies expand our knowledge of the role of lipid metabolism in A. phagocytophilum infection and multiplication in HL-60 cells and suggest a mechanism by which A. phagocytophilum modulates lipid metabolism...
- Mapping of B-cell epitopes in the N-terminal repeated peptides of Anaplasma marginale major surface protein 1a and characterization of the humoral immune response of cattle immunized with recombinant and whole organism antigens
Jose C Garcia-Garcia
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, 250 McElroy Hall, Stillwater, OK 74078-2007, USA
Vet Immunol Immunopathol 98:137-51. 2004
..These results provide insight into the bovine antibody response against A. marginale and the role of MSP1a in protection of cattle against A. marginale infection...
- Molecular cloning and characterisation of the griffon vulture (Gyps fulvus) toll-like receptor 1
José Manuel Pérez de la Lastra
Instituto de Investigación en Recursos Cinegéticos UCLM CSIC JCCLM, Ronda Toledo s n, 13071 Ciudad Real, Spain
Dev Comp Immunol 31:511-9. 2007
....
- Adaptations of the tick-borne pathogen, Anaplasma marginale, for survival in cattle and ticks
Katherine M Kocan
Department of Veterinary Pathobiology, 250 McElroy Hall, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078 2007, USA
Exp Appl Acarol 28:9-25. 2002
..Infection and development of A. marginale in cattle and tick cells appears to differ and to be mediated by several surface proteins encoded from the small genome...
- Advances toward understanding the molecular biology of the Anaplasma-tick interface
Katherine M Kocan
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
Front Biosci 13:7032-45. 2008
..Collectively, this information will likely lead to the development of dual target vaccines designed to protect vertebrates against tick infestations and prevent the transmission of pathogens...
- Differential expression of the msp1alpha gene of Anaplasma marginale occurs in bovine erythrocytes and tick cells
Jose C Garcia-Garcia
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA
Vet Microbiol 98:261-72. 2004
..marginale for host cells. Variation in the expression of MSP1a may also contribute to phenotypic and antigenic changes in the pathogen...
- Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris
Mario Canales
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, 13071 Ciudad Real, Spain
BMC Biotechnol 8:14. 2008
..The R. microplus Bm86 protective antigen has been produced by recombinant DNA technology and shown to protect cattle against tick infestations...
- RNA interference for the study and genetic manipulation of ticks
Jose De La Fuente
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
Trends Parasitol 23:427-33. 2007
..It is probable that the knowledge gained from this experimental approach will contribute to development of vaccines to control tick infestations and the transmission of tick-borne pathogens...
- Applications of a cell culture system for studying the interaction of Anaplasma marginale with tick cells
Edmour F Blouin
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078 2007, USA
Anim Health Res Rev 3:57-68. 2002
..marginale-tick interactions. Information gained from these studies may be applicable to other closely related tick-borne pathogens that have been propagated in the same tick cell line...
- Anaplasma marginale major surface protein 1a directs cell surface display of tick BM95 immunogenic peptides on Escherichia coli
Mario Canales
Instituto de Investigacion en Recursos Cinegeticos IREC CSIC UCLM JCCM, Ronda de Toledo s n, 13071 Ciudad Real, Spain
J Biotechnol 135:326-32. 2008
..This system provides a novel approach to the surface display of heterologous antigenic proteins on live E. coli and suggests the possibility to use the recombinant bacteria for immunization studies against cattle tick infestations...
- Co-feeding studies of ticks infected with Anaplasma marginale
Katherine M Kocan
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA
Vet Parasitol 112:295-305. 2003
..marginale transmission. A. marginale infections in male ticks were not increased while co-feeding with females. Thus, co-feeding of adult Dermacentor spp. does not appear to influence the dynamics of A. marginale transmission...
- Antigens and alternatives for control of Anaplasma marginale infection in cattle
Katherine M Kocan
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, Oklahoma 74078, USA
Clin Microbiol Rev 16:698-712. 2003
..marginale, combined with the new technologies, should contribute to the development of more effective vaccines for control and prevention of anaplasmosis...
- Effect of tetracycline on development of Anaplasma marginale in cultured Ixodes scapularis cells
Edmour F Blouin
Department of Veterinary Pathobiology, 250 McElroy Hall, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA
Vet Parasitol 107:115-26. 2002
..marginale in cultured tick cells. The Anaplasma-tick cell culture drug assay therefore, would be useful for screening and evaluating novel antibiotics for control of anaplasmosis...
- Glycosylation of Anaplasma marginale major surface protein 1a and its putative role in adhesion to tick cells
Jose C Garcia-Garcia
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, Oklahoma 74078-2007, USA
Infect Immun 72:3022-30. 2004
..These results demonstrated that MSP1a and MSP1b are glycosylated and suggest that the glycosylation of MSP1a plays a role in the adhesion of A. marginale to tick cells...
- Molecular epidemiology of human and bovine anaplasmosis in southern Europe
Victoria Naranjo
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
Ann N Y Acad Sci 1078:95-9. 2006
..The presence of concurrent infections in cattle and deer suggests that these pathogens may multiply in the same reservoir host and illustrates the complexity of the epidemiology of bovine and human anaplasmosis in this region...
- Targeting the tick-pathogen interface for novel control strategies
Jose De La Fuente
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
Front Biosci 13:6947-56. 2008
..Use of these molecular approaches will likely contribute to control measures that will notably reduce tick populations and tick-borne diseases in the future...
- Capillary tube feeding system for studying tick-pathogen interactions of Dermacentor variabilis (Acari: Ixodidae) and Anaplasma marginale (Rickettsiales: Anaplasmataceae)
Katherine M Kocan
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078-2007, USA
J Med Entomol 42:864-74. 2005
....
- Vaccination with recombinant tick antigens for the control of Ixodes scapularis adult infestations
Consuelo Almazan
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, 250 McElroy Hall, Oklahoma State University, Stillwater, OK 74078, USA
Vaccine 23:5294-8. 2005
..These antigens, and especially 4D8, appear to be good candidates for continued development of a vaccine for control of tick infestations...
- Genes differentially expressed in oropharyngeal tonsils and mandibular lymph nodes of tuberculous and nontuberculous European wild boars naturally exposed to Mycobacterium bovis
Victoria Naranjo
, Ronda de Toledo, Ciudad Real, Spain
FEMS Immunol Med Microbiol 46:298-312. 2006
..bovis, and provide a basis for defining host-pathogen interactions and the mechanism of protective immunity...
- Identification of protective antigens for the control of Ixodes scapularis infestations using cDNA expression library immunization
Consuelo Almazan
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078, USA
Vaccine 21:1492-501. 2003
..Our studies demonstrated for the first time that cDNA expression library immunization (ELI) combined with sequence analysis is a powerful and efficient tool for identification of candidate antigens for use in vaccines against ticks...
