Jose De La Fuente



  1. Villar M, Ayllón N, Alberdi P, Moreno A, Moreno M, Tobes R, et al. Integrated Metabolomics, Transcriptomics and Proteomics Identifies Metabolic Pathways Affected by Anaplasma phagocytophilum Infection in Tick Cells. Mol Cell Proteomics. 2015;14:3154-72 pubmed publisher
    ..These results support the use of this experimental approach to systematically identify cell pathways and molecular mechanisms involved in tick-pathogen interactions. Data are available via ProteomeXchange with identifier PXD002181. ..
  2. Cabezas Cruz A, Espinosa P, Alberdi P, Simo L, Valdes J, Mateos Hernández L, et al. Tick galactosyltransferases are involved in ?-Gal synthesis and play a role during Anaplasma phagocytophilum infection and Ixodes scapularis tick vector development. Sci Rep. 2018;8:14224 pubmed publisher
  3. Carvajal de la Fuente V, Merino Charrez O, Tovar Carman E, Rodríguez Camarillo S, Lagunes Quintanilla R, Muñoz Tenería F, et al. Differential expression analysis for subolesin in Rhipicephalus microplus infected with Anaplasma marginale. Exp Appl Acarol. 2018;76:229-241 pubmed publisher
    ..marginale. Results from this study revealed that, the expression of subolesin was significantly higher in salivary glands of infected R. microplus in comparison to uninfected ones. ..
  4. Risalde M, López V, Contreras M, Mateos Hernández L, Gortazar C, De La Fuente J. Control of mycobacteriosis in zebrafish (Danio rerio) mucosally vaccinated with heat-inactivated Mycobacterium bovis. Vaccine. 2018;36:4447-4453 pubmed publisher
    ..bovis IV in zebrafish was based on the activation of the innate immune response through the C3 pathway. These results support the use of the M. bovis IV administered by immersion for the control of mycobacteriosis in fish. ..
  5. de la Fuente J, Estrada Peña A, Cabezas Cruz A, Brey R. Flying ticks: anciently evolved associations that constitute a risk of infectious disease spread. Parasit Vectors. 2015;8:538 pubmed publisher
    ..To implement effective measures for the control of tick-borne diseases, it is necessary to study bird-tick and bird-pathogen molecular interactions including the immune response of birds to tick infestation and pathogen infection. ..
  6. Cabezas Cruz A, Valdés J, de la Fuente J. The glycoprotein TRP36 of Ehrlichia sp. UFMG-EV and related cattle pathogen Ehrlichia sp. UFMT-BV evolved from a highly variable clade of E. canis under adaptive diversifying selection. Parasit Vectors. 2014;7:584 pubmed publisher
    ..Our results suggest that Ehrlichia sp. UFMG-EV and Ehrlichia sp. UFMT-BV evolved from a variable clade within E. canis. ..
  7. Silva J, Cabezas Cruz A, Fonseca A, Barbosa J, de la Fuente J. Infection of water buffalo in Rio de Janeiro Brazil with Anaplasma marginale strains also reported in cattle. Vet Parasitol. 2014;205:730-4 pubmed publisher
    ..The results of this study suggested that water buffalo in this region are naturally infected with the same strains of A. marginale found in cattle. ..
  8. Hernández Jarguín A, Díaz Sánchez S, Villar M, De La Fuente J. Integrated metatranscriptomics and metaproteomics for the characterization of bacterial microbiota in unfed Ixodes ricinus. Ticks Tick Borne Dis. 2018;9:1241-1251 pubmed publisher
    ..Metaomics approaches integrating different omics datasets would provide a better description of tick microbiota compositions, and insights into tick interactions with microbiota, pathogens and hosts. ..
  9. Silva J, Fonseca A, Barbosa J, Cabezas Cruz A, de la Fuente J. Low genetic diversity associated with low prevalence of Anaplasma marginale in water buffaloes in Marajó Island, Brazil. Ticks Tick Borne Dis. 2014;5:801-4 pubmed publisher
    ..The results also suggested that mechanical transmission and not biological transmission by ticks might be playing the major role for pathogen circulation among water buffaloes in Marajó Island, Brazil. ..

More Information


  1. request reprint
    De La Fuente J, Kocan K. Advances in the identification and characterization of protective antigens for recombinant vaccines against tick infestations. Expert Rev Vaccines. 2003;2:583-93 pubmed
    ..Design of future tick vaccines should target multiple tick species, as well as interfere with the transmission of pathogens. ..
  2. De La Fuente J. Controlling ticks and tick-borne diseases…looking forward. Ticks Tick Borne Dis. 2018;9:1354-1357 pubmed publisher
    ..This paper is not a review of current approaches for the control of ticks and TBDs, but an opinion about future directions in this area. ..
  3. De La Fuente J, Gortazar C, Vicente J, Villar M. Host expression of methylmalonyl-CoA mutase and tuberculosis: a missing link?. Med Hypotheses. 2011;76:361-4 pubmed publisher
    ..If the hypothesis is proven true, these results have important implications for the prevention and treatment of bTB in humans and for the eradication of bTB in wildlife reservoir hosts. ..
  4. de la Fuente J, Villar M, Contreras M, Moreno Cid J, Merino O, Pérez de la Lastra J, et al. Prospects for vaccination against the ticks of pets and the potential impact on pathogen transmission. Vet Parasitol. 2015;208:26-9 pubmed publisher
    ..The growing interaction between pets and people underlines the importance of developing new interventions for the monitoring and control of VBD. ..
  5. Lis K, Fernández de Mera I, Popara M, Cabezas Cruz A, Ayllón N, Zweygarth E, et al. Molecular and immunological characterization of three strains of Anaplasma marginale grown in cultured tick cells. Ticks Tick Borne Dis. 2015;6:522-9 pubmed publisher
    ..marginale are antigenically conserved, even after several in vitro passages, supporting the use of some of the above conserved proteins as candidates for universal vaccines. ..
  6. request reprint
    de la Fuente J, Garcia Garcia J, Blouin E, Kocan K. Differential adhesion of major surface proteins 1a and 1b of the ehrlichial cattle pathogen Anaplasma marginale to bovine erythrocytes and tick cells. Int J Parasitol. 2001;31:145-53 pubmed
    ..marginale adhesin for both bovine erythrocytes and tick cells while MSP1b is an adhesin only for bovine erythrocytes. The role of the MSP1 complex, therefore, appears to vary among vertebrate and invertebrate hosts. ..
  7. Hornok S, Kontschán J, Estrada Peña A, de Mera I, Tomanović S, de la Fuente J. Contributions to the morphology and phylogeny of the newly discovered bat tick species, Ixodes ariadnae in comparison with I. vespertilionis and I. simplex. Parasit Vectors. 2015;8:47 pubmed publisher
    ..The present study illustrates the morphology and clarifies the phylogenetic relationships of the three known bat tick species that occur in Europe. According to its subolesin gene I. ariadnae may have a long evolutionary history. ..
  8. de la Fuente J, Díez Delgado I, Contreras M, Vicente J, Cabezas Cruz A, Manrique M, et al. Complete Genome Sequences of Field Isolates of Mycobacterium bovis and Mycobacterium caprae. Genome Announc. 2015;3: pubmed publisher
    ..The genomes of three M. bovis (MB1, MB3, MB4) and one M. caprae (MB2) field isolates with different virulence, prevalence, and host distribution phenotypes were sequenced. ..
  9. Weisheit S, Villar M, Tykalová H, Popara M, Loecherbach J, Watson M, et al. Ixodes scapularis and Ixodes ricinus tick cell lines respond to infection with tick-borne encephalitis virus: transcriptomic and proteomic analysis. Parasit Vectors. 2015;8:599 pubmed publisher
  10. Kocan K, de la Fuente J, Coburn L. Insights into the development of Ixodes scapularis: a resource for research on a medically important tick species. Parasit Vectors. 2015;8:592 pubmed publisher
  11. De La Fuente J, Garcia Garcia J, Blouin E, Saliki J, Kocan K. Infection of tick cells and bovine erythrocytes with one genotype of the intracellular ehrlichia Anaplasma marginale excludes infection with other genotypes. Clin Diagn Lab Immunol. 2002;9:658-68 pubmed
    ..marginale in bovine erythrocytes and tick cells, resulting in the establishment of only one genotype, and appears to be the first report of infection exclusion for Anaplasma and Ehrlichia species. ..
  12. Chaligiannis Ι, Fernandez de Mera I, Papa A, Sotiraki S, De La Fuente J. Molecular identification of tick-borne pathogens in ticks collected from dogs and small ruminants from Greece. Exp Appl Acarol. 2018;74:443-453 pubmed publisher
    ..The results of this study demonstrate the variety of tick-borne pathogens of animal and human importance circulating in Greece, and that awareness is needed to minimize the risk of infection, especially among farmers and pet owners. ..
  13. Hornok S, Estrada Peña A, Kontschán J, Plantard O, Kunz B, Mihalca A, et al. High degree of mitochondrial gene heterogeneity in the bat tick species Ixodes vespertilionis, I. ariadnae and I. simplex from Eurasia. Parasit Vectors. 2015;8:457 pubmed publisher
    ..Data of this study also reflect that I. vespertilionis may represent a species complex. ..
  14. request reprint
    De La Fuente J, Almazan C, Blouin E, Naranjo V, Kocan K. Reduction of tick infections with Anaplasma marginale and A. phagocytophilum by targeting the tick protective antigen subolesin. Parasitol Res. 2006;100:85-91 pubmed
    ..These results suggest that subolesin appears to be a candidate vaccine antigen that may contribute to control of multiple tick species and the reduction of tick-borne pathogens. ..
  15. Cabezas Cruz A, Mateos Hernández L, Pérez Cruz M, Valdés J, Mera I, Villar M, et al. Regulation of the Immune Response to α-Gal and Vector-borne Diseases. Trends Parasitol. 2015;31:470-6 pubmed publisher
  16. request reprint
    De La Fuente J, Passos L, Van Den Bussche R, Ribeiro M, Facury Filho E, Kocan K. Genetic diversity and molecular phylogeny of Anaplasma marginale isolates from Minas Gerais, Brazil. Vet Parasitol. 2004;121:307-16 pubmed
    ..marginale isolates, while MSP1a was found not to be a useful marker for phylogeographic characterization of A. marginale isolates. ..
  17. De La Fuente J, Kocan K, Blouin E, Zivkovic Z, Naranjo V, Almazan C, et al. Functional genomics and evolution of tick-Anaplasma interactions and vaccine development. Vet Parasitol. 2010;167:175-86 pubmed publisher
  18. Cabezas Cruz A, de la Fuente J. Anaplasma marginale major surface protein 1a: a marker of strain diversity with implications for control of bovine anaplasmosis. Ticks Tick Borne Dis. 2015;6:205-10 pubmed publisher
    ..marginale strain diversity as it relates geographic and ecological factors and to the development of effective vaccines for control of bovine anaplasmosis worldwide. ..
  19. Pinheiro Silva R, Borges L, Coelho L, Cabezas Cruz A, Valdés J, do Rosário V, et al. Gene expression changes in the salivary glands of Anopheles coluzzii elicited by Plasmodium berghei infection. Parasit Vectors. 2015;8:485 pubmed publisher
    ..coluzzii salivary glands infection by Plasmodium parasite, working as a sporozoite receptor and/or promoting a favorable environment for the capacity of sporozoites. ..
  20. Doncel Pérez E, Mateos Hernández L, Pareja E, García Forcada Ã, Villar M, Tobes R, et al. Expression of Early Growth Response Gene-2 and Regulated Cytokines Correlates with Recovery from Guillain-Barré Syndrome. J Immunol. 2016;196:1102-7 pubmed publisher
    ..These results identified biomarkers associated with GBS recovery and suggested that EGR2 overexpression has a pivotal role in the downregulation of cytokines implicated in the pathophysiology of this acute neuropathy. ..
  21. request reprint
    de la Fuente J, Torina A, Naranjo V, Caracappa S, Vicente J, Mangold A, et al. Genetic diversity of Anaplasma marginale strains from cattle farms in the province of Palermo, Sicily. J Vet Med B Infect Dis Vet Public Health. 2005;52:226-9 pubmed
    ..These results suggest that development of farm husbandry systems and vaccines for genetically heterogeneous populations of A. marginale are needed for control of anaplasmosis in this region of Sicily. ..
  22. de la Fuente J, Estrada Peña A, Cabezas Cruz A, Kocan K. Anaplasma phagocytophilum Uses Common Strategies for Infection of Ticks and Vertebrate Hosts. Trends Microbiol. 2016;24:173-180 pubmed publisher
    ..The discovery of these common mechanisms provides evidence that a control strategy could be developed targeted at both vertebrate and tick hosts for more complete control of A. phagocytophilum and its associated diseases. ..
  23. request reprint
    De La Fuente J, Ruiz Fons F, Naranjo V, Torina A, Rodriguez O, Gortazar C. Evidence of Anaplasma infections in European roe deer (Capreolus capreolus) from southern Spain. Res Vet Sci. 2008;84:382-6 pubmed
    ..ovis and A. phagocytophilum in Spain and suggest that this species may be involved in the natural cycle of these pathogens in this region, thus acting as potential reservoir for transmission to domestic and wild animals. ..
  24. Contreras M, Moreno Cid J, Domingos A, Canales M, Díez Delgado I, Pérez de la Lastra J, et al. Bacterial membranes enhance the immunogenicity and protective capacity of the surface exposed tick Subolesin-Anaplasma marginale MSP1a chimeric antigen. Ticks Tick Borne Dis. 2015;6:820-8 pubmed publisher
    ..These results provide further support for the development of tick vaccines using E. coli membranes exposing chimeric antigens such as SUB-MSP1a. ..
  25. Cabezas Cruz A, Zweygarth E, Broniszweska M, Passos L, Ribeiro M, Manrique M, et al. Complete Genome Sequence of Ehrlichia mineirensis, a Novel Organism Closely Related to Ehrlichia canis with a New Host Association. Genome Announc. 2015;3: pubmed publisher
    ..E. mineirensis is the best characterized Ehrlichia isolate from a novel cattle-related clade closely related to the monocytotropic pathogen E. canis. ..
  26. Contreras M, Villar M, Artigas Jerónimo S, Kornieieva L, Mуtrofanov S, De La Fuente J. A reverse vaccinology approach to the identification and characterization of Ctenocephalides felis candidate protective antigens for the control of cat flea infestations. Parasit Vectors. 2018;11:43 pubmed publisher
    ..These results support the development of vaccines with protective antigens affecting flea reproduction and development after feeding on immunized animals for the control of cat flea infestations. ..
  27. Cabezas Cruz A, Mateos Hernández L, Chmelař J, Villar M, de la Fuente J. Salivary Prostaglandin E2: Role in Tick-Induced Allergy to Red Meat. Trends Parasitol. 2017;33:495-498 pubmed publisher
    ..Immune tolerance to α-Gal in blood type B individuals might reduce the risk to this allergy. ..
  28. Blanda V, Torina A, La Russa F, D Agostino R, Randazzo K, Scimeca S, et al. A retrospective study of the characterization of Rickettsia species in ticks collected from humans. Ticks Tick Borne Dis. 2017;8:610-614 pubmed publisher
    ..The Rickettsia spp. reported in this study were identified in previously recognized or new potential tick vectors in Europe, highlighting the risk of infection by different Rickettsia spp. for humans bitten by ticks in Sicily. ..
  29. López V, Alberdi P, Fernández de Mera I, Barasona J, Vicente J, Garrido J, et al. Evidence of co-infection with Mycobacterium bovis and tick-borne pathogens in a naturally infected sheep flock. Ticks Tick Borne Dis. 2016;7:384-9 pubmed publisher
    ..bovis may results in unique gene expression patterns in sheep. The results underline the need for further research into the possible role of sheep in the epidemiology of animal tuberculosis. ..
  30. Díaz Sánchez S, Hernández Jarguín A, Fernández de Mera I, Alberdi P, Zweygarth E, Gortazar C, et al. Draft Genome Sequences of Anaplasma phagocytophilum, A. marginale, and A. ovis Isolates from Different Hosts. Genome Announc. 2018;6: pubmed publisher
    ..phagocytophilum (human), A. marginale (cattle), and A. ovis (goat) isolates from the United States were sequenced and characterized. This is the first report of an A. ovis genome sequence. ..
  31. Schetters T, Bishop R, Crampton M, Kopáček P, Lew Tabor A, Maritz Olivier C, et al. Cattle tick vaccine researchers join forces in CATVAC. Parasit Vectors. 2016;9:105 pubmed publisher
    ..A meeting sponsored by the Bill & Melinda Gates Foundation was held at the Avanti Hotel, Mohammedia, Morocco, July 14-15, 2015. The meeting resulted in the formation of the Cattle Tick Vaccine Consortium (CATVAC). ..
  32. Contreras M, Villar M, Alberdi P, De La Fuente J. Vaccinomics Approach to Tick Vaccine Development. Methods Mol Biol. 2016;1404:275-86 pubmed publisher
  33. request reprint
    De La Fuente J, Almazan C, Van Den Bussche R, Bowman J, Yoshioka J, Kocan K. Characterization of genetic diversity in Dermacentor andersoni (Acari: Ixodidae) with body size and weight polymorphism. Exp Parasitol. 2005;109:16-26 pubmed
    ..The results reported herein show that B ticks have a better reproductive success than S ticks and suggest partial reproductive isolation of S ticks. ..
  34. Torres L, Almazan C, Ayllón N, Galindo R, Rosario Cruz R, Quiroz Romero H, et al. Functional genomics of the horn fly, Haematobia irritans (Linnaeus, 1758). BMC Genomics. 2011;12:105 pubmed publisher
    ..The aim of this study was to conduct a functional genomics study in female horn flies using Expressed Sequence Tags (EST) analysis and RNA interference (RNAi)...
  35. Zivkovic Z, Esteves E, Almazan C, Daffre S, Nijhof A, Kocan K, et al. Differential expression of genes in salivary glands of male Rhipicephalus (Boophilus)microplus in response to infection with Anaplasma marginale. BMC Genomics. 2010;11:186 pubmed publisher
    ..microplus male salivary glands in response to A. marginale infection. Additionally, a R. microplus-derived cell line, BME26, was used for the first time to also study tick cell gene expression in response to A. marginale infection...
  36. Kocan K, Zivkovic Z, Blouin E, Naranjo V, Almazan C, Mitra R, et al. Silencing of genes involved in Anaplasma marginale-tick interactions affects the pathogen developmental cycle in Dermacentor variabilis. BMC Dev Biol. 2009;9:42 pubmed publisher
    ..marginale developmental cycle. These four genes encoded for putative glutathione S-transferase (GST), salivary selenoprotein M (SelM), H+ transporting lysosomal vacuolar proton pump (vATPase) and subolesin...
  37. Estrada Pena A, Naranjo V, Acevedo Whitehouse K, Mangold A, Kocan K, De La Fuente J. Phylogeographic analysis reveals association of tick-borne pathogen, Anaplasma marginale, MSP1a sequences with ecological traits affecting tick vector performance. BMC Biol. 2009;7:57 pubmed publisher
    ..marginale-tick co-evolution, these studies have not provided phylogeographic information on a global scale because of the high level of MSP1a genetic diversity among geographic strains...
  38. de la Fuente J, Contreras M, Estrada Peña A, Cabezas Cruz A. Targeting a global health problem: Vaccine design and challenges for the control of tick-borne diseases. Vaccine. 2017;35:5089-5094 pubmed publisher
    ..Nevertheless, vaccines constitute the most safe and effective intervention for the control of TBDs in humans, domestic and wild animals. ..
  39. request reprint
    Cabezas Cruz A, Alberdi P, Valdes J, Villar M, De La Fuente J. Remodeling of tick cytoskeleton in response to infection with Anaplasma phagocytophilum. Front Biosci (Landmark Ed). 2017;22:1830-1844 pubmed
    ..These results provided a more comprehensive view of the cytoskeletal components involved in the response to A. phagocytophilum infection in ticks. ..
  40. request reprint
    De La Fuente J, Vicente J, Höfle U, Ruiz Fons F, Fernandez de Mera I, Van Den Bussche R, et al. Anaplasma infection in free-ranging Iberian red deer in the region of Castilla-La Mancha, Spain. Vet Microbiol. 2004;100:163-73 pubmed
    ..marginatum and Rhipicephalus bursa were identified as potential biological vectors for A. marginale in this region and may effect transmission of A. marginale between deer and cattle populations...
  41. request reprint
    De La Fuente J, Ruybal P, Mtshali M, Naranjo V, Shuqing L, Mangold A, et al. Analysis of world strains of Anaplasma marginale using major surface protein 1a repeat sequences. Vet Microbiol. 2007;119:382-90 pubmed
    ..marginale strains from various geographic locations worldwide. These results contribute to the understanding of the genetic diversity and evolution of A. marginale and tick-pathogen interactions...
  42. request reprint
    De La Fuente J, Naranjo V, Ruiz Fons F, Höfle U, Fernandez de Mera I, Villanúa D, et al. Potential vertebrate reservoir hosts and invertebrate vectors of Anaplasma marginale and A. phagocytophilum in central Spain. Vector Borne Zoonotic Dis. 2005;5:390-401 pubmed
    ..The increasing contact between wildlife, domestic animals, and human populations increases the risk of outbreaks of human and bovine anaplasmosis, and the difficulty of implementing surveillance and control measures...
  43. Alberdi P, Espinosa P, Cabezas Cruz A, De La Fuente J. Anaplasma phagocytophilum Manipulates Host Cell Apoptosis by Different Mechanisms to Establish Infection. Vet Sci. 2016;3: pubmed publisher
    ..These results suggest that A. phagocytophilum uses different mechanisms to inhibit apoptosis for infection of both vertebrate and invertebrate hosts. ..
  44. Villar M, López V, Ayllón N, Cabezas Cruz A, Lopez J, Vazquez J, et al. The intracellular bacterium Anaplasma phagocytophilum selectively manipulates the levels of vertebrate host proteins in the tick vector Ixodes scapularis. Parasit Vectors. 2016;9:467 pubmed publisher
    ..These results have implications for the identification of new targets for the development of vaccines for the control of tick-borne diseases. ..
  45. De La Fuente J, Van Den Bussche R, Prado T, Kocan K. Anaplasma marginale msp1alpha genotypes evolved under positive selection pressure but are not markers for geographic isolates. J Clin Microbiol. 2003;41:1609-16 pubmed
    ..marginale within Oklahoma could be explained by cattle movement and the maintenance of different genotypes by independent transmission events...
  46. request reprint
    De La Fuente J, Garcia Garcia J, Blouin E, Kocan K. Characterization of the functional domain of major surface protein 1a involved in adhesion of the rickettsia Anaplasma marginale to host cells. Vet Microbiol. 2003;91:265-83 pubmed
    ..These studies demonstrated that the tandemly repeated region of the protein mediates the adhesive function of MSP1a...
  47. request reprint
    De La Fuente J, Kocan K, Garcia Garcia J, Blouin E, Claypool P, Saliki J. Vaccination of cattle with Anaplasma marginale derived from tick cell culture and bovine erythrocytes followed by challenge-exposure with infected ticks. Vet Microbiol. 2002;89:239-251 pubmed
    ..The cell culture-derived A. marginale shows promise for use as antigen in development of a new killed vaccine for anaplasmosis...
  48. request reprint
    De La Fuente J, Lew A, Lutz H, Meli M, Hofmann Lehmann R, Shkap V, et al. Genetic diversity of anaplasma species major surface proteins and implications for anaplasmosis serodiagnosis and vaccine development. Anim Health Res Rev. 2005;6:75-89 pubmed
    ..Information concerning the genetic diversity of Anaplasma spp. MSPs will likely influence the development of serodiagnostic assays and vaccine strategies for the control of anaplasmosis...
  49. request reprint
    De La Fuente J, Almazan C, Naranjo V, Blouin E, Meyer J, Kocan K. Autocidal control of ticks by silencing of a single gene by RNA interference. Biochem Biophys Res Commun. 2006;344:332-8 pubmed
    ..Conservation of subolesin among tick species suggests that SAT may be useful for control of many medically and economically important tick species...
  50. request reprint
    De La Fuente J, Atkinson M, Hogg J, Miller D, Naranjo V, Almazan C, et al. Genetic characterization of Anaplasma ovis strains from bighorn sheep in Montana. J Wildl Dis. 2006;42:381-5 pubmed
    ..marginale in cattle herds in the western United States and worldwide. These results demonstrated that bighorn sheep may be a wildlife reservoir of A. ovis in Montana...
  51. request reprint
    De La Fuente J, Atkinson M, Naranjo V, Fernandez de Mera I, Mangold A, Keating K, et al. Sequence analysis of the msp4 gene of Anaplasma ovis strains. Vet Microbiol. 2007;119:375-81 pubmed
    ..The results reported herein further confirm that A. ovis infection occurs in natural wild ruminant populations in Western United States and that bighorn sheep and mule deer may serve as wildlife reservoirs of A. ovis...
  52. request reprint
    De La Fuente J, Ayoubi P, Blouin E, Almazan C, Naranjo V, Kocan K. Anaplasmosis: focusing on host-vector-pathogen interactions for vaccine development. Ann N Y Acad Sci. 2006;1078:416-23 pubmed
    ..The screening for tick-protective antigens resulted in vaccine candidates reducing tick infestation, molting, and oviposition and affecting Anaplasma infection levels in ticks...
  53. request reprint
    De La Fuente J, Almazan C, Canales M, Pérez de la Lastra J, Kocan K, Willadsen P. A ten-year review of commercial vaccine performance for control of tick infestations on cattle. Anim Health Res Rev. 2007;8:23-8 pubmed
  54. request reprint
    de la Fuente J, Garcia Garcia J, Blouin E, Rodriguez S, Garcia M, Kocan K. Evolution and function of tandem repeats in the major surface protein 1a of the ehrlichial pathogen Anaplasma marginale. Anim Health Res Rev. 2001;2:163-73 pubmed
    ..These repeated sequences include markers for tick transmissibility that may be important in the identification of ehrlichial pathogens because they may influence control strategies and the design of subunit vaccines...
  55. request reprint
    de la Fuente J, Garcia Garcia J, Blouin E, McEwen B, Clawson D, Kocan K. Major surface protein 1a effects tick infection and transmission of Anaplasma marginale. Int J Parasitol. 2001;31:1705-14 pubmed
    ..marginale by Dermacentor spp. ticks. The mechanism of tick infection and transmission of A. marginale is important in formulating control strategies and development of improved vaccines for anaplasmosis...
  56. request reprint
    de la Fuente J, Van Den Bussche R, Kocan K. Molecular phylogeny and biogeography of North American isolates of Anaplasma marginale (Rickettsiaceae: Ehrlichieae). Vet Parasitol. 2001;97:65-76 pubmed
    ..variabilis. These phylogenetic analyses provide information that may be important to consider when developing control strategies for anaplasmosis in the United States...
  57. de la Fuente J, Massung R, Wong S, Chu F, Lutz H, Meli M, et al. Sequence analysis of the msp4 gene of Anaplasma phagocytophilum strains. J Clin Microbiol. 2005;43:1309-17 pubmed publisher
    ..These results suggest that A. phagocytophilum strains from ruminants could share some common characteristics, including reservoirs and pathogenicity, which may be different from strains that infect humans...
  58. request reprint
    De La Fuente J, Van Den Bussche R, Garcia Garcia J, Rodríguez S, Garcia M, Guglielmone A, et al. Phylogeography of New World isolates of Anaplasma marginale based on major surface protein sequences. Vet Microbiol. 2002;88:275-85 pubmed
    ..marginale isolates. In contrast MSP1a sequences appeared to be rapidly evolving and these sequences may provide phylogeographic information only when numerous isolate MSP1a sequences are analyzed from a geographic area...
  59. De La Fuente J, Blouin E, Kocan K. Infection exclusion of the rickettsial pathogen anaplasma marginale in the tick vector Dermacentor variabilis. Clin Diagn Lab Immunol. 2003;10:182-4 pubmed
    ..These studies demonstrate that infection exclusion of A. marginale genotypes also occurs in naturally infected ticks, as well as in cattle and cultured tick cells, and results in establishment of only one genotype per tick...
  60. Fernandez de Mera I, Blanda V, Torina A, Dabaja M, El Romeh A, Cabezas Cruz A, et al. Identification and molecular characterization of spotted fever group rickettsiae in ticks collected from farm ruminants in Lebanon. Ticks Tick Borne Dis. 2018;9:104-108 pubmed publisher
    ..Therefore, these results suggested further investigation on the vector competence of the tick species with unknown role in transmission of some of the pathogens identified in this study. ..
  61. request reprint
    De La Fuente J, Garcia Garcia J, Barbet A, Blouin E, Kocan K. Adhesion of outer membrane proteins containing tandem repeats of Anaplasma and Ehrlichia species (Rickettsiales: Anaplasmataceae) to tick cells. Vet Microbiol. 2004;98:313-22 pubmed
    ..The results reported herein provide new information on the role of E. ruminantium "mucin" as an adhesin for tick cells and also suggest a role of glycans in adhesin molecules...
  62. Contreras M, De La Fuente J. Control of infestations by Ixodes ricinus tick larvae in rabbits vaccinated with aquaporin recombinant antigens. Vaccine. 2017;35:1323-1328 pubmed publisher
    ..microplus AQP1 on the control of cattle tick infestations. These results supports that AQP, and particularly CoAQP, might be a candidate protective antigen for the control of different tick species. ..
  63. Contreras M, De La Fuente J. Control of Ixodes ricinus and Dermacentor reticulatus tick infestations in rabbits vaccinated with the Q38 Subolesin/Akirin chimera. Vaccine. 2016;34:3010-3013 pubmed publisher
    ..The effect of the Q38 vaccine on larval feeding and molting is essential to reduce tick infestations and supports that Q38 might be a candidate universal antigen for the control of multiple tick species that can infest the same host. ..
  64. request reprint
    De La Fuente J, Ayoubi P, Blouin E, Almazan C, Naranjo V, Kocan K. Gene expression profiling of human promyelocytic cells in response to infection with Anaplasma phagocytophilum. Cell Microbiol. 2005;7:549-59 pubmed
    ..phagocytophilum-infected HL-60 cells, thus expanding in a global manner the existing information on the response of mammalian cells to A. phagocytophilum infection...
  65. request reprint
    De La Fuente J, Almazan C, Blouin E, Naranjo V, Kocan K. RNA interference screening in ticks for identification of protective antigens. Parasitol Res. 2005;96:137-41 pubmed
  66. request reprint
    De La Fuente J, Torina A, Caracappa S, Tumino G, Furlá R, Almazan C, et al. Serologic and molecular characterization of Anaplasma species infection in farm animals and ticks from Sicily. Vet Parasitol. 2005;133:357-62 pubmed
    ..Definition of the diversity of Anaplasma spp. in Sicily reported, herein is fundamental to development of control strategies for A. marginale, A. ovis and A. phagocytophilum in Sicily...
  67. request reprint
    De La Fuente J, Almazan C, Naranjo V, Blouin E, Kocan K. Synergistic effect of silencing the expression of tick protective antigens 4D8 and Rs86 in Rhipicephalus sanguineus by RNA interference. Parasitol Res. 2006;99:108-13 pubmed
    ..These results demonstrated the synergistic effect of the silencing expression of two tick protective genes. Inclusion of multiple tick protective antigens may, therefore, enhance the efficacy of tick vaccines...
  68. request reprint
    De La Fuente J, Blouin E, Manzano Román R, Naranjo V, Almazan C, Pérez de la Lastra J, et al. Functional genomic studies of tick cells in response to infection with the cattle pathogen, Anaplasma marginale. Genomics. 2007;90:712-22 pubmed
    ..The results of these studies demonstrated that a molecular mechanism occurs by which tick cell gene expression mediates the A. marginale developmental cycle and trafficking through ticks...
  69. De La Fuente J, Blouin E, Manzano Román R, Naranjo V, Almazan C, Pérez de la Lastra J, et al. Differential expression of the tick protective antigen subolesin in anaplasma marginale- and A. phagocytophilum-infected host cells. Ann N Y Acad Sci. 2008;1149:27-35 pubmed publisher
    ..The results reported herein further support the role of subolesin in Anaplasma-host interactions and suggest a putative role of subolesin in vaccines for the control of pathogen infection/multiplication in ticks...
  70. De La Fuente J, Manzano Román R, Naranjo V, Kocan K, Zivkovic Z, Blouin E, et al. Identification of protective antigens by RNA interference for control of the lone star tick, Amblyomma americanum. Vaccine. 2010;28:1786-95 pubmed publisher