Ivan Rychlik

Summary

Affiliation: Veterinary Research Institute
Country: Czech Republic

Publications

  1. pmc Gene expression in the chicken caecum in response to infections with non-typhoid Salmonella
    Ivan Rychlik
    Veterinary Research Institute, Hudcova 70, Brno, 621 00, Czech Republic
    Vet Res 45:119. 2014
  2. pmc The local immune response of mice after Helicobacter suis infection: strain differences and distinction with Helicobacter pylori
    Bram Flahou
    Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke, 9820, Belgium
    Vet Res 43:75. 2012
  3. pmc Chicken innate immune response to oral infection with Salmonella enterica serovar Enteritidis
    Marta Matulova
    Veterinary Research Institute, Hudcova 70, Brno 621 00, Czech Republic
    Vet Res 44:37. 2013
  4. pmc SPI-1-encoded type III secretion system of Salmonella enterica is required for the suppression of porcine alveolar macrophage cytokine expression
    Barbora Pavlova
    Veterinary Research Institute, Brno, Czech Republic
    Vet Res 42:16. 2011
  5. ncbi request reprint Salmonella stress management and its relevance to behaviour during intestinal colonisation and infection
    Ivan Rychlik
    Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic
    FEMS Microbiol Rev 29:1021-40. 2005
  6. ncbi request reprint Distribution and function of plasmids in Salmonella enterica
    I Rychlik
    Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic
    Vet Microbiol 112:1-10. 2006
  7. ncbi request reprint Computer-assisted restriction endonuclease analysis of plasmid DNA in field strains of Salmonella enteritidis
    I Rychlik
    Veterinary Research Institute, Brno, Czech Republic
    Can J Microbiol 44:1183-5. 1998
  8. doi request reprint Salmonella enterica serovar Typhimurium typing by prophage-specific PCR
    Ivan Rychlik
    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
    Microbiology 154:1384-9. 2008
  9. pmc Virulence potential of five major pathogenicity islands (SPI-1 to SPI-5) of Salmonella enterica serovar Enteritidis for chickens
    Ivan Rychlik
    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
    BMC Microbiol 9:268. 2009
  10. pmc Low-molecular-weight plasmid of Salmonella enterica serovar Enteritidis codes for retron reverse transcriptase and influences phage resistance
    I Rychlik
    Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic
    J Bacteriol 183:2852-8. 2001

Collaborators

Detail Information

Publications67

  1. pmc Gene expression in the chicken caecum in response to infections with non-typhoid Salmonella
    Ivan Rychlik
    Veterinary Research Institute, Hudcova 70, Brno, 621 00, Czech Republic
    Vet Res 45:119. 2014
    ..This review outlines the function of individual proteins expressed in chickens after infection with non-typhoid Salmonella serovars...
  2. pmc The local immune response of mice after Helicobacter suis infection: strain differences and distinction with Helicobacter pylori
    Bram Flahou
    Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke, 9820, Belgium
    Vet Res 43:75. 2012
    ..suis strains and the immune response they evoke. A predominant Th17 response was observed, accompanied by a mild Th2 response, which is different from the Th17/Th1 response evoked by H. pylori infection...
  3. pmc Chicken innate immune response to oral infection with Salmonella enterica serovar Enteritidis
    Marta Matulova
    Veterinary Research Institute, Hudcova 70, Brno 621 00, Czech Republic
    Vet Res 44:37. 2013
    ..This points to an important role for immunoglobulins in the restoration of homeostasis in the cecum after infection...
  4. pmc SPI-1-encoded type III secretion system of Salmonella enterica is required for the suppression of porcine alveolar macrophage cytokine expression
    Barbora Pavlova
    Veterinary Research Institute, Brno, Czech Republic
    Vet Res 42:16. 2011
    ..Enteritidis. Our results showed that the SPI-1-encoded T3SS is required not only for cell invasion but in macrophages also for the suppression of early proinflammatory cytokine expression...
  5. ncbi request reprint Salmonella stress management and its relevance to behaviour during intestinal colonisation and infection
    Ivan Rychlik
    Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic
    FEMS Microbiol Rev 29:1021-40. 2005
    ..The role of quorum-sensing through luxS and sdiA is also considered as a regulator of virulence and colonisation...
  6. ncbi request reprint Distribution and function of plasmids in Salmonella enterica
    I Rychlik
    Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic
    Vet Microbiol 112:1-10. 2006
    ..Despite limited knowledge on their function, their presence or absence is frequently used for strain differentiation in epidemiological studies...
  7. ncbi request reprint Computer-assisted restriction endonuclease analysis of plasmid DNA in field strains of Salmonella enteritidis
    I Rychlik
    Veterinary Research Institute, Brno, Czech Republic
    Can J Microbiol 44:1183-5. 1998
    ..enteritidis. In addition to plasmid-free and virulence plasmid only containing strains, 15 additional plasmid types were detected. All the images and detailed protocols are available at the Web site http://www.clark.cz/vri/salmon.htm...
  8. doi request reprint Salmonella enterica serovar Typhimurium typing by prophage-specific PCR
    Ivan Rychlik
    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
    Microbiology 154:1384-9. 2008
    ..Typhimurium...
  9. pmc Virulence potential of five major pathogenicity islands (SPI-1 to SPI-5) of Salmonella enterica serovar Enteritidis for chickens
    Ivan Rychlik
    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
    BMC Microbiol 9:268. 2009
    ..e. with the remaining four deleted) and a mutant with all 5 SPIs deleted, and assessed their virulence in one-day-old chickens, together with the innate immune response of this host...
  10. pmc Low-molecular-weight plasmid of Salmonella enterica serovar Enteritidis codes for retron reverse transcriptase and influences phage resistance
    I Rychlik
    Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic
    J Bacteriol 183:2852-8. 2001
    ..This plasmid was also able to confer phage resistance onto the host cell by a mechanism which was independent of sdsDNA synthesis...
  11. ncbi request reprint Flow cytometry characterisation of Salmonella typhimurium mutants defective in proton translocating proteins and stationary-phase growth phenotype
    I Rychlik
    Veterinary Research Institute, Hudcova 70, 621 32, Brno, Czech Republic
    J Microbiol Methods 42:255-63. 2000
    ....
  12. ncbi request reprint Subdivision of Salmonella enterica serovar enteritidis phage types PT14b and PT21 by plasmid profiling
    I Rychlik
    Veterinary Research Institute, Hudcova 70, 621 32, Brno, Czech Republic
    Vet Microbiol 74:217-25. 2000
    ..This indicates that low molecular weight plasmids in Salmonella enteritidis are not responsible for the spread of antibiotic resistance...
  13. ncbi request reprint Rapid detection of Salmonella in field samples by nested polymerase chain reaction
    I Rychlik
    Veterinary Research Institute, Hudcova, Brno, Czech Republic
    Lett Appl Microbiol 29:269-72. 1999
    ....
  14. pmc Cytokine signaling in splenic leukocytes from vaccinated and non-vaccinated chickens after intravenous infection with Salmonella enteritidis
    Marta Matulova
    Veterinary Research Institute, Brno, Czech Republic
    PLoS ONE 7:e32346. 2012
    ..However, when the expression of IL17 was lower than that of IFNγ as in the vaccinated chickens, the Th1 response with a higher resistance to S. Enteritidis infection dominated...
  15. pmc Influence of 5 major Salmonella pathogenicity islands on NK cell depletion in mice infected with Salmonella enterica serovar Enteritidis
    Daniela Karasova
    Veterinary Research Institute, Hudcova 70, 62100 Brno, Czech Republic
    BMC Microbiol 10:75. 2010
    ..In this study we were interested in the colonisation and early immune response of Balb/C mice to infection with Salmonella Enteritidis and isogenic pathogenicity island free mutants...
  16. pmc Characterization of chicken spleen transcriptome after infection with Salmonella enterica serovar Enteritidis
    Marta Matulova
    Veterinary Research Institute, Brno, Czech Republic
    PLoS ONE 7:e48101. 2012
    ..Expression of AVD, EXFABP, IRG1, AH221, TRAP6, SAA and all immunoglobulin genes can be therefore used for the characterization of the course of S. Enteritidis infection in chickens...
  17. ncbi request reprint Identification of Salmonella enterica serovar Typhimurium genes associated with growth suppression in stationary-phase nutrient broth cultures and in the chicken intestine
    Ivan Rychlik
    Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic
    Arch Microbiol 178:411-20. 2002
    ..enterica sv. Typhimurium culture, suggesting that the gene belongs to a new sensing and signaling regulatory pathway in S. enterica sv. Typhimurium...
  18. pmc Immune response of chicken gut to natural colonization by gut microflora and to Salmonella enterica serovar enteritidis infection
    Magdalena Crhanova
    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
    Infect Immun 79:2755-63. 2011
    ..Active modification of chicken gut microbiota in the future may accelerate or potentiate the maturation of the gut immune system and increase its resistance to infection with different pathogens...
  19. doi request reprint Epidemiology and interaction of Salmonella enterica serovar Derby, Infantis and Typhimurium with porcine alveolar macrophages
    Jiri Volf
    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
    Vet Microbiol 146:105-10. 2010
    ..The lower cytokine signaling but higher cytotoxicity of S. Typhimurium for macrophages correlates with the higher virulence for pigs of this serotype when compared with S. Derby or S. Infantis...
  20. doi request reprint SPI1 defective mutants of Salmonella enterica induce cross-protective immunity in chickens against challenge with serovars Typhimurium and Enteritidis
    Marta Matulova
    Veterinary Research Institute, Brno, Czech Republic
    Vaccine 31:3156-62. 2013
    ..e. the mixed vaccine provided an additional protective effect when compared with the chickens vaccinated with a vaccine formed by only a single Salmonella serovar...
  21. doi request reprint Immune response of pigs to Salmonella enterica serovar Derby and Typhimurium infections
    Jan Matiasovic
    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic Electronic address
    Vet Microbiol 170:284-90. 2014
    ..Cellular immune response to both strains was very low and was detected later than was the onset of IgG antibody production. ..
  22. doi request reprint Impact of maternally-derived antibodies against Salmonella enterica serovar Typhimurium on the bacterial load in suckling piglets
    Jan Matiasovic
    Veterinary Research Institute, Hudcova 70, Brno, Czech Republic
    Vet J 196:114-5. 2013
    ..Such a vaccine, delivering colostral/lactogenic immunity to piglets thus has the potential to reduce the prevalence non-typhoid Salmonella spp. infection...
  23. ncbi request reprint Retron reverse transcriptase (rrtT) can be lost in multidrug resistant Salmonella enterica serovar Typhimurium DT 104 strains and influences virulence for mice
    Lenka Pilousova
    Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic
    Vet Microbiol 111:191-7. 2005
    ..Since the retron excision occurred exclusively in multidrug resistant S. typhimurium, it cannot be excluded that such strains may increase their virulence in the future...
  24. pmc Vaccination of chickens with SPI1-lon and SPI1-lon-fliC mutant of Salmonella enterica Serovar Enteritidis
    Marta Matulova
    Veterinary Research Institute, Brno, Czech Republic
    PLoS ONE 8:e66172. 2013
    ..The SPI1-lon-fliC mutant is therefore a suitable marker vaccine strain for oral vaccination of poultry...
  25. pmc SPI-1 encoded genes of Salmonella Typhimurium influence differential polarization of porcine alveolar macrophages in vitro
    Kamila Kyrova
    Veterinary Research Institute, Hudcova Brno, Czech Republic
    BMC Vet Res 8:115. 2012
    ..Some pathogens are capable of interfering with differentiation in order to down-regulate the anti-microbial activity and enhance their survival in the host...
  26. ncbi request reprint Salmonella enterica subsp. enterica serovar Enteritidis harbours ColE1, ColE2, and rolling-circle-like replicating plasmids
    Daniela Gregorova
    Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic
    Can J Microbiol 50:107-12. 2004
    ....
  27. pmc aro mutations in Salmonella enterica cause defects in cell wall and outer membrane integrity
    Alena Sebkova
    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
    J Bacteriol 190:3155-60. 2008
    ....
  28. doi request reprint Association of attenuated mutants of Salmonella enterica serovar Enteritidis with porcine peripheral blood leukocytes
    Hana Stepanova
    Veterinary Research Institute, Brno, Czech Republic
    FEMS Microbiol Lett 321:37-42. 2011
    ..Enteritidis with porcine leukocytes. This information can be used for the design of live Salmonella vaccines preferentially targeting particular cell types including cancer or tumor cells...
  29. pmc Influence of Salmonella enterica serovar Enteritidis infection on the composition of chicken cecal microbiota
    Petra Videnska
    Veterinary Research Institute, Brno, Czech Republic
    BMC Vet Res 9:140. 2013
    ..To reach this aim, we compared the cecal microbiota of non-infected chickens and those infected by S. Enteritidis by pyrosequencing the V3/V4 variable regions of genes coding for 16S rRNA...
  30. doi request reprint allB, allantoin utilisation and Salmonella enterica serovar Enteritidis and Typhimurium colonisation of poultry and mice
    Jitka Matiasovicova
    Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic
    Folia Microbiol (Praha) 56:264-9. 2011
    ..The decrease in virulence of the allB mutant was relatively minor as it could be observed only after a mixed infection model, consistent with a lower prevalence, but not a total absence of such clones in poultry flocks...
  31. pmc Transient and Prolonged Response of Chicken Cecum Mucosa to Colonization with Different Gut Microbiota
    Jiri Volf
    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
    PLoS ONE 11:e0163932. 2016
    ....
  32. pmc Immune protection of chickens conferred by a vaccine consisting of attenuated strains of Salmonella Enteritidis, Typhimurium and Infantis
    Karolina Varmuzova
    Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic
    Vet Res 47:94. 2016
    ..Adjusting the vaccine composition and changing the administration route therefore affects vaccine efficacy...
  33. doi request reprint Antibiotic Resistance, Core-Genome and Protein Expression in IncHI1 Plasmids in Salmonella Typhimurium
    Tereza Kubasova
    Veterinary Research Institute, Hudcova 70, Brno 621 00, Czech Republic
    Genome Biol Evol 8:1661-71. 2016
    ..This is the likely mechanism that facilitates host cell survival when antibiotics suddenly emerge in the environment. ..
  34. doi request reprint Cytokine mRNA expression in porcine cell lines stimulated by enterotoxigenic Escherichia coli
    Barbora Pavlova
    Veterinary Research Institute, Hudcova 70, Brno 621 00, Czech Republic
    Vet Microbiol 132:105-10. 2008
    ..The highest IL-8 and TNF-alpha mRNA expression in 3D4/31 was detected after stimulation with serotype O149:K88 frequently associated with hemorrhagic gastroenteritis...
  35. ncbi request reprint Retron reverse transcriptase rrtT is ubiquitous in strains of Salmonella enterica serovar Typhimurium
    Jitka Matiasovicova
    Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic
    FEMS Microbiol Lett 223:281-6. 2003
    ..Detailed computer analysis allowed us to predict the sequence of msDNA and to propose that the final msDNA is free of any RNA. Furthermore, we predict that there are at least three different classes of retron reverse transcriptases...
  36. doi request reprint Characterization of Salmonella enterica serovar Typhimurium conjugative plasmids transferring resistance to antibiotics and their interaction with the virulence plasmid
    H Hradecka
    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
    J Antimicrob Chemother 62:938-41. 2008
    ..In this study, we analysed field isolates of Salmonella enterica serovar Typhimurium for the presence of conjugative plasmids transferring resistances to antibiotics...
  37. doi request reprint Curcuma and Scutellaria plant extracts protect chickens against inflammation and Salmonella Enteritidis infection
    Karolina Varmuzova
    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
    Poult Sci 94:2049-58. 2015
    ..The supplementation of chicken feed with extracts from Curcuma and Scutelleria thus may be used in poultry production to effectively decrease gut inflammation and increase chicken performance. ..
  38. doi request reprint Influence of the lipopolysaccharide structure of Salmonella enterica serovar Enteritidis on interactions with pig neutrophils
    Jan Matiasovic
    Veterinary Research Institute, Hudcova 70, Brno 621 00, Czech Republic
    Vet Microbiol 150:167-72. 2011
    ....
  39. pmc The response of porcine monocyte derived macrophages and dendritic cells to Salmonella Typhimurium and lipopolysaccharide
    Kamila Kyrova
    Veterinary Research Institute, Hudcova 70, Brno 621 00, Czech Republic
    BMC Vet Res 10:244. 2014
    ..To avoid any bias, the expression was determined by protein LC-MS/MS and verified at the level of transcription by quantitative RT-PCR...
  40. pmc Chicken faecal microbiota and disturbances induced by single or repeated therapy with tetracycline and streptomycin
    Petra Videnska
    Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic
    BMC Vet Res 9:30. 2013
    ..This enabled us to characterise dynamics of the changes after antibiotic administration and withdrawal, and to identify genera repeatedly resistant to tetracycline and streptomycin...
  41. ncbi request reprint Role of SdiA in Salmonella enterica serovar Typhimurium physiology and virulence
    Jiri Volf
    Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic
    Arch Microbiol 178:94-101. 2002
    ..entericasv. Typhimurium mutant. sdiA, dually controlled by iron concentration and culture-density-derived signals, may therefore play an important role in S. entericasv. Typhimurium virulence regulation...
  42. doi request reprint Deletion of sodCI and spvBC in Salmonella enterica serovar Enteritidis reduced its virulence to the natural virulence of serovars Agona, Hadar and Infantis for mice but not for chickens early after infection
    D Karasova
    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
    Vet Microbiol 139:304-9. 2009
    ..Enteritidis virulence for chickens directly, they may be of an indirect effect through the increased persistence of S. Enteritidis in mice and increased probability of the reintroduction of S. Enteritidis into poultry flocks...
  43. doi request reprint Distribution of integrons and SGI1 among antibiotic-resistant Salmonella enterica isolates of animal origin
    H Havlickova
    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
    Vet Microbiol 133:193-8. 2009
    ..Sandiego and S. Pullorum, dfrA1 and aadA1 gene cassettes in S. Typhimurium integron, and aadA21 gene cassette in S. Braenderub and S. Zanzibar...
  44. pmc Characterization of Antibiotic Resistance Gene Abundance and Microbiota Composition in Feces of Organic and Conventional Pigs from Four EU Countries
    Lenka Gerzova
    Veterinary Research Institute, Brno, Czech Republic
    PLoS ONE 10:e0132892. 2015
    ..Therefore, the geographical location of the herd influenced the antibiotic resistance in the fecal microbiota more than farm's status as organic or conventional. ..
  45. pmc phoP, SPI1, SPI2 and aroA mutants of Salmonella Enteritidis induce a different immune response in chickens
    Marta Elsheimer-Matulova
    Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic
    Vet Res 46:96. 2015
    ..The differences in interaction between the mutants and chicken host can be used for a more detailed understanding of the chicken immune system. ..
  46. pmc Important Metabolic Pathways and Biological Processes Expressed by Chicken Cecal Microbiota
    Ondrej Polansky
    Veterinary Research Institute, Brno, Czech RepublicINRS Institut Armand Frappier
    Appl Environ Microbiol 82:1569-76. 2015
    ..However, certain care should be taken during microbiota transplantation because the microbiota may behave differently in the intestinal tract of a recipient depending on how well the existing communities are established. ..
  47. pmc Succession and replacement of bacterial populations in the caecum of egg laying hens over their whole life
    Petra Videnska
    Veterinary Research Institute, Brno, Czech Republic
    PLoS ONE 9:e115142. 2014
    ..The fourth stage was typical for adult hens in full egg production aged 7 months or more and was characterised by a constant ratio of Bacteroidetes and Firmicutes formed by equal numbers of the representatives of both phyla. ..
  48. ncbi request reprint Differentiation of Actinobacillus pleuropneumoniae by PCR-REA based on sequence variability of the apxIVA gene and by ribotyping
    Zoran Jaglic
    Veterinary Research Institute Brno, Hudcova 70, 621 32 Brno, Czech Republic
    Vet Microbiol 103:63-9. 2004
    ..Field isolates with serotype cross-reactivity 9, 11, and/or 1 could not be differentiated by either of these methods...
  49. pmc The early innate response of chickens to Salmonella enterica is dependent on the presence of O-antigen but not on serovar classification
    Karolina Varmuzova
    Veterinary Research Institute, Brno, Czech Republic
    PLoS ONE 9:e96116. 2014
    ....
  50. doi request reprint Retron Se72 utilizes a unique strategy of the self-priming initiation of reverse transcription
    Lenka Pilousova
    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
    Cell Mol Life Sci 68:3607-17. 2011
    ..Due to the relaxed demands for the initiation of reverse transcription, RrtE has the potential to generate dsDNA from different RNA transcripts in vivo...
  51. doi request reprint aroA and aroD mutations influence biofilm formation in Salmonella Enteritidis
    Marcela Malcova
    Veterinary Research Institute, Brno, Czech Republic
    FEMS Microbiol Lett 291:44-9. 2009
    ..aroA and aroD containing plasmids complemented all the defects of the aro mutants. Beside its attenuation for different hosts, the loss of ability to form biofilm is an additional interesting characteristic of aro mutants...
  52. ncbi request reprint The presence of genes homologous to the K88 genes faeH and faeI on the virulence plasmid of Salmonella gallinarum
    I Rychlik
    Veterinary Research Institute, Brno, Czech Republic
    FEMS Microbiol Lett 159:255-60. 1998
    ..A similar region of homology was found by DNA-DNA hybridization on the virulence plasmids of S. pullorum, S. dublin and other S. gallinarum strains but not in the plasmids of S. typhimurium, S. enteritidis or S. choleraesuis...
  53. ncbi request reprint Plasmid pC present in Salmonella enterica serovar Enteritidis PT14b strains encodes a restriction modification system
    Daniela Gregorova
    Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic
    FEMS Microbiol Lett 214:195-8. 2002
    ..coli. Using insertional mutagenesis we confirmed experimentally that the plasmid pC-encoded restriction modification system was functional and could explain the high resistance of S. Enteritidis PT14b strains to phage infection...
  54. ncbi request reprint Standardisation of restriction fragment length polymorphism analysis for Mycobacterium avium subspecies paratuberculosis
    I Pavlik
    Veterinary Research Institute, Brno, Czech Republic
    J Microbiol Methods 38:155-67. 1999
    ..A combination of both RFLP (PstI) and RFLP (BstEII) results revealed a total of 28 different RFLP types. All the RFLP types and detailed protocols are available at Intemet web site WWW...: http:/ /www.vri.cz/wwwrflptext.htm...
  55. pmc Relationship between IS901 in the Mycobacterium avium complex strains isolated from birds, animals, humans, and the environment and virulence for poultry
    I Pavlik
    Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic
    Clin Diagn Lab Immunol 7:212-7. 2000
    ..The remaining 111 strains, of serotypes 4 to 28, were nonvirulent and did not incorporate IS901. None of the 152 strains isolated from humans was virulent for poultry, including 12 strains which were IS901 positive...
  56. ncbi request reprint Identification of putative ancestors of the multidrug-resistant Salmonella enterica serovar typhimurium DT104 clone harboring the Salmonella genomic island 1
    J Matiasovicova
    Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic
    Arch Microbiol 187:415-24. 2007
    ..We therefore propose that a lineage of the S. typhimurium DT104 sensitive strain first lost the ability to metabolize allantoin and then acquired SGI1...
  57. pmc Bipartite Graphs for Visualization Analysis of Microbiome Data
    Karel Sedlar
    Department of Biomedical Engineering, Brno University of Technology, Brno, Czech Republic
    Evol Bioinform Online 12:17-23. 2016
    ..Capturing labels in the vertices also brings the possibility of clearly comparing two or more microbial communities by showing their common and unique parts. ..
  58. pmc Characterization of egg laying hen and broiler fecal microbiota in poultry farms in Croatia, Czech Republic, Hungary and Slovenia
    Petra Videnska
    Veterinary Research Institute, Brno, Czech Republic
    PLoS ONE 9:e110076. 2014
    ....
  59. doi request reprint Comparative analysis of Salmonella enterica serovar Enteritidis mutants with a vaccine potential
    D Karasova
    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
    Vaccine 27:5265-70. 2009
    ..Furthermore, when a detailed comparison of polymyxin and serum sensitive mutants was performed, the serum sensitive mutants were more immunogenic...
  60. ncbi request reprint Cytokine response of porcine cell lines to Salmonella enterica serovar typhimurium and its hilA and ssrA mutants
    J Volf
    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
    Zoonoses Public Health 54:286-93. 2007
    ..The ssrA mutant can therefore be considered as more suitable for further vaccine development as the stimulation of innate immune response is important for animal protection against a challenge with virulent strains...
  61. ncbi request reprint Ordered expression of virulence genes in Salmonella enterica serovar typhimurium
    K Papezova
    Veterinary Research Institute, 621 00 Brno, Czechia
    Folia Microbiol (Praha) 52:107-14. 2007
    ..Among SPI-1 genes, those with regulatory functions preceded in expression the effector genes and sop genes were expressed in the order of sopA, sopB and sopE2, showing hierarchy in the expression of S. Typhimurium virulence genes...
  62. pmc Evolution of antibiotic resistance in Salmonella enterica serovar typhimurium strains isolated in the Czech Republic between 1984 and 2002
    M Faldynova
    Veterinary Research Institute, 621 32 Brno, Czech Republic
    Antimicrob Agents Chemother 47:2002-5. 2003
    ..A new variant of the aadA gene designated aadA21 is described, the 5' end of which was identical to aadA2 and the 3' end of which was identical to aadA1...
  63. pmc An outbreak of human salmonellosis caused by ampicillin-resistant Salmonella enterica serovar Enteritidis PT13 in the Czech Republic
    H Hradecka
    Veterinary Research Institute, Hudcova, Brno, Czech Republic
    Epidemiol Infect 134:737-40. 2006
    ..Our results indicate that all S. Enteritidis PT13 tested from 2004 belonged to a single outbreak strain which prior to 2004 had not been recognized in the Czech Republic...
  64. pmc Housing Systems Influence Gut Microbiota Composition of Sows but Not of Their Piglets
    Tereza Kubasova
    Veterinary Research Institute, Hudcova, Brno, Czech Republic
    PLoS ONE 12:e0170051. 2017
    ..The microbiota of 1- and 4-day-old piglets differed from the microbiota of sows and sows therefore did not represent the most important source for their colonization in early days of life...
  65. pmc Characterization of microbiota composition and presence of selected antibiotic resistance genes in carriage water of ornamental fish
    Lenka Gerzova
    Veterinary Research Institute, Brno, Czech Republic
    PLoS ONE 9:e103865. 2014
    ....
  66. doi request reprint Biofilm formation in field strains of Salmonella enterica serovar Typhimurium: identification of a new colony morphology type and the role of SGI1 in biofilm formation
    M Malcova
    Veterinary Research Institute, Brno, Czech Republic
    Vet Microbiol 129:360-6. 2008
    ..Due to a mucoid and brown appearance on Congo Red agar we designated these strains as belonging to the SBAM (smooth brown and mucoid) morphotype...
  67. doi request reprint Complete genome analysis of two new bacteriophages isolated from impetigo strains of Staphylococcus aureus
    Tibor Botka
    Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlarska 2, 611 37, Brno, Czech Republic
    Virus Genes 51:122-31. 2015
    ..Thus, both phages represent two new lineages of as yet not characterized bacteriophages of the Siphoviridae family having impact on pathogenicity of impetigo strains of S. aureus...