Affiliation: Palacky University
Location: Olomouc, Czech Republic
M.D. in 1990, Ph.D. in 1997, works part-time as principal investigator and part-time as routine mycologist in University Hospital.
My aim is to work for rapid translation of new scientific knowledge into clinical microbiology practice for the benefit of patient. I also strive to supplement this translational research by basic research focused on medically relevant topics in yeasts. Individual topics pursued are as follows:
Hypoxia-response in Cryptococcus neoformans
Ability to adapt to oxygen limitation was identified as a signal for unbudded G2-arrest in this obligate aerobic pathogenic yeast. Furthermore, a role of hypoxia response in cryptococcal virulence has emerged recently. Therefore, we focus on characterisation of cryptococcal hypoxia response useful for development of a cultivating system that should serve as a model for cryptococcal growth in human tissue.
Identification and typing of medically relevant yeasts
Recently, the technology of high resolution melting analysis of double-stranded DNA (typically PCR amplicons) is gradually more affordable to routine clinical microbiology laboratories. Therefore, we focus on developing competitive amplification systems which should best cover the whole spectrum of medically important yeast, enabling for their rapid species identification and possibly also strain typing in a convenient automated or semi-automated way. The McRAPD and PCR-HRMA approaches are at the center of our efforts, including development of multi-purpose software QAS useful for quantitative comparison of complex melting data (melting curves).
- Trtkova J, Raclavsky V. Molecular-genetic approaches to identification and typing of pathogenic Candida yeasts. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2006;150:51-61 pubmed
- Raclavsky V, Pavlicek J, Novotny R, Moranova Z, Ohkusu M, Trtkova J, et al. Peculiar clusters of daughter cells observed in Cryptococcus neoformans grown in sealed microtiter plates. Folia Microbiol (Praha). 2009;54:369-71 pubmed publisher..However, their daughters were unable to separate from them and to continue their proliferation under such conditions. ..
- Raclavsky V, Novotny R, Stary L, Navrátilová L, Zatloukal J, Jakubec P, et al. NAS agar is more suitable than McKay agar for primary culture of Streptococcus milleri group (SMG) fastidious bacteria, S. intermedius in particular. Folia Microbiol (Praha). 2017;62:11-15 pubmed publisher..Therefore, when economic conditions are not limiting and maximum recovery rate is desirable, both plates are advised to be used in parallel for primary cultivation of clinical samples. ..