David Smajs

Summary

Affiliation: Masaryk University
Country: Czech Republic

Publications

  1. pmc Bacteriocin-encoding genes and ExPEC virulence determinants are associated in human fecal Escherichia coli strains
    Lenka Micenková
    Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, Brno 625 00, Czech Republic
    BMC Microbiol 14:109. 2014
  2. pmc Structure of rrn operons in pathogenic non-cultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains
    Darina Čejková
    Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, 62500 Brno, Czech Republic
    J Med Microbiol 62:196-207. 2013
  3. pmc Genome analysis of Treponema pallidum subsp. pallidum and subsp. pertenue strains: most of the genetic differences are localized in six regions
    Lenka Mikalová
    Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
    PLoS ONE 5:e15713. 2010
  4. pmc Whole genome sequence of the Treponema Fribourg-Blanc: unspecified simian isolate is highly similar to the yaws subspecies
    Marie Zobaníková
    Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
    PLoS Negl Trop Dis 7:e2172. 2013
  5. pmc Complete genome sequence of Treponema pallidum strain DAL-1
    Marie Zobaníková
    Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
    Stand Genomic Sci 7:12-21. 2012
  6. pmc Genetic diversity in Treponema pallidum: implications for pathogenesis, evolution and molecular diagnostics of syphilis and yaws
    David Smajs
    Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, 625 00 Brno, Czech Republic
    Infect Genet Evol 12:191-202. 2012
  7. pmc Bacteriocin synthesis in uropathogenic and commensal Escherichia coli: colicin E1 is a potential virulence factor
    David Smajs
    Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Brno, Czech Republic
    BMC Microbiol 10:288. 2010
  8. ncbi request reprint Complete sequence of low-copy-number plasmid MccC7-H22 of probiotic Escherichia coli H22 and the prevalence of mcc genes among human E. coli
    David Smajs
    Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, 625 00 Brno, Czech Republic
    Plasmid 59:1-10. 2008
  9. pmc Complete genome sequence of Treponema paraluiscuniculi, strain Cuniculi A: the loss of infectivity to humans is associated with genome decay
    David Smajs
    Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
    PLoS ONE 6:e20415. 2011
  10. ncbi request reprint Experimental administration of the probiotic Escherichia coli strain Nissle 1917 results in decreased diversity of E. coli strains in pigs
    David Smajs
    Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Brno, Czech Republic
    Curr Microbiol 64:205-10. 2012

Collaborators

Detail Information

Publications35

  1. pmc Bacteriocin-encoding genes and ExPEC virulence determinants are associated in human fecal Escherichia coli strains
    Lenka Micenková
    Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, Brno 625 00, Czech Republic
    BMC Microbiol 14:109. 2014
    ..coli strains of human fecal origin isolated in the South Moravia region of the Czech Republic was collected during the years 2007-2010. Altogether, 17 virulence determinants and 31 bacteriocin-encoding genes were tested in each of them...
  2. pmc Structure of rrn operons in pathogenic non-cultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains
    Darina Čejková
    Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, 62500 Brno, Czech Republic
    J Med Microbiol 62:196-207. 2013
    ..It is suggested that the random distribution of tRNA genes is caused by reciprocal translocation between repetitive sequences mediated by a recBCD-like system...
  3. pmc Genome analysis of Treponema pallidum subsp. pallidum and subsp. pertenue strains: most of the genetic differences are localized in six regions
    Lenka Mikalová
    Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
    PLoS ONE 5:e15713. 2010
    ..p. pallidum and T. p. pertenue strains. These regions of sequence divergence could be used for the molecular detection and discrimination of syphilis and yaws strains...
  4. pmc Whole genome sequence of the Treponema Fribourg-Blanc: unspecified simian isolate is highly similar to the yaws subspecies
    Marie Zobaníková
    Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
    PLoS Negl Trop Dis 7:e2172. 2013
    ..This strain was morphologically indistinguishable from T. pallidum ssp. pallidum or ssp. pertenue strains, and it was shown to cause human infections...
  5. pmc Complete genome sequence of Treponema pallidum strain DAL-1
    Marie Zobaníková
    Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
    Stand Genomic Sci 7:12-21. 2012
    ..pallidum strain Nichols. The comparison of the complete DAL-1 genome sequence with the Nichols sequence revealed a list of genetic differences that are potentially responsible for the increased rabbit virulence of the DAL-1 strain...
  6. pmc Genetic diversity in Treponema pallidum: implications for pathogenesis, evolution and molecular diagnostics of syphilis and yaws
    David Smajs
    Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, 625 00 Brno, Czech Republic
    Infect Genet Evol 12:191-202. 2012
    ..Genome analyses also shed light on treponemal evolution and on chromosomal targets for molecular diagnostics of treponemal infections...
  7. pmc Bacteriocin synthesis in uropathogenic and commensal Escherichia coli: colicin E1 is a potential virulence factor
    David Smajs
    Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Brno, Czech Republic
    BMC Microbiol 10:288. 2010
    ..coli phylogroups were tested in a set of 361 E. coli strains isolated from human urinary tract infections (UTI) and in 411 control strains isolated from feces of patients without bacterial gut infection...
  8. ncbi request reprint Complete sequence of low-copy-number plasmid MccC7-H22 of probiotic Escherichia coli H22 and the prevalence of mcc genes among human E. coli
    David Smajs
    Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, 625 00 Brno, Czech Republic
    Plasmid 59:1-10. 2008
    ..coli H22. Screening of 105 E. coli strains isolated from human fecal samples revealed 2 (1.9%) strains that produced microcin C7...
  9. pmc Complete genome sequence of Treponema paraluiscuniculi, strain Cuniculi A: the loss of infectivity to humans is associated with genome decay
    David Smajs
    Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
    PLoS ONE 6:e20415. 2011
    ..The genes that are inactivated or altered in T. paraluiscuniculi are candidates for virulence factors important in the infectivity and pathogenesis of T. pallidum subspecies...
  10. ncbi request reprint Experimental administration of the probiotic Escherichia coli strain Nissle 1917 results in decreased diversity of E. coli strains in pigs
    David Smajs
    Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Brno, Czech Republic
    Curr Microbiol 64:205-10. 2012
    ..The experimental administration of EcN strain was not sufficient for stable colonization of porcine gut, but induced significant changes in the enterobacterial microbiota...
  11. ncbi request reprint Recognition of pore-forming colicin Y by its cognate immunity protein
    David Smajs
    Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
    FEMS Microbiol Lett 258:108-13. 2006
    ..Mutations in cui, which converted Cui sequence to Cyi sequence in positions 104, 107, 110, 112 and 159, resulted in an immunity gene that also conferred (besides immunity to colicin U) a high degree of immunity to colicin Y...
  12. pmc Transcriptome of Treponema pallidum: gene expression profile during experimental rabbit infection
    David Smajs
    Human Genome Sequencing Center, University of Texas Houston Medical School, Houston, Texas, USA
    J Bacteriol 187:1866-74. 2005
    ..This characterization of the T. pallidum transcriptome during experimental infection provides further insight into the importance of gene expression levels for the survival and pathogenesis of this bacterium...
  13. ncbi request reprint Inactivation of colicin Y by intramembrane helix-helix interaction with its immunity protein
    David Smajs
    Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
    FEBS J 275:5325-31. 2008
    ..These data are consistent with antiparallel helix-helix interactions between Cyi helix T3 and Cya helix 8 of the pore-forming domain as a molecular mechanism of colicin Y inactivation by its immunity protein...
  14. pmc Whole genome sequence of Treponema pallidum ssp. pallidum, strain Mexico A, suggests recombination between yaws and syphilis strains
    Helena Pětrošová
    Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
    PLoS Negl Trop Dis 6:e1832. 2012
    ..Attempts to cultivate TPA Mexico A strain under in vitro conditions have revealed lower growth potential compared to other tested TPA strains...
  15. pmc A novel Treponema pallidum antigen, TP0136, is an outer membrane protein that binds human fibronectin
    Mary Beth Brinkman
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Infect Immun 76:1848-57. 2008
    ..These results demonstrate that TP0136 is expressed on the outer membrane of the treponeme during infection and may be involved in attachment to host extracellular matrix components...
  16. pmc Complete genome sequence of Treponema pallidum ssp. pallidum strain SS14 determined with oligonucleotide arrays
    Petra Matejková
    Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Alkek N1619, Houston, TX 77030, USA
    BMC Microbiol 8:76. 2008
    ..pallidum remains the enigmatic pathogen, since no virulence factors have been identified and the pathogenesis of the disease is poorly understood. Increasing rates of new syphilis cases per year have been observed recently...
  17. pmc Resequencing of Treponema pallidum ssp. pallidum strains Nichols and SS14: correction of sequencing errors resulted in increased separation of syphilis treponeme subclusters
    Helena Pětrošová
    Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
    PLoS ONE 8:e74319. 2013
    ..Since publication of their complete genome sequences, a number of sequencing errors in each genome have been reported. Therefore, we have resequenced TPA Nichols and SS14 strains using next-generation sequencing techniques...
  18. pmc Genome differences between Treponema pallidum subsp. pallidum strain Nichols and T. paraluiscuniculi strain Cuniculi A
    Michal Strouhal
    Department of Biology, Building A6, Faculty of Medicine, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
    Infect Immun 75:5859-66. 2007
    ..6 to 99.3%. These results are consistent with a close genetic relationship among the T. pallidum strains and subspecies and a strong, but relatively divergent connection between the human and rabbit pathogens...
  19. pmc Whole genome sequences of three Treponema pallidum ssp. pertenue strains: yaws and syphilis treponemes differ in less than 0.2% of the genome sequence
    Darina Čejková
    Department of Biology, Masaryk University, Brno, Czech Republic
    PLoS Negl Trop Dis 6:e1471. 2012
    ..pallidum (TPA). Both yaws and syphilis are distinguished on the basis of epidemiological characteristics, clinical symptoms, and several genetic signatures of the corresponding causative agents...
  20. doi request reprint Macrolide treatment failure in a case of secondary syphilis: a novel A2059G mutation in the 23S rRNA gene of Treponema pallidum subsp. pallidum
    Petra Matejková
    Department of Biology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic
    J Med Microbiol 58:832-6. 2009
    ..The prevalence of A2058G and A2059G mutations among clinical specimens was 18.2 and 18.2 %, respectively...
  21. pmc Novel colicin Fy of Yersinia frederiksenii inhibits pathogenic Yersinia strains via YiuR-mediated reception, TonB import, and cell membrane pore formation
    Juraj Bosák
    Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
    J Bacteriol 194:1950-9. 2012
    ....
  22. pmc Comparison of CDC and sequence-based molecular typing of syphilis treponemes: tpr and arp loci are variable in multiple samples from the same patient
    Lenka Mikalová
    Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
    BMC Microbiol 13:178. 2013
    ..We tested whether identified molecular subtypes were identical in PCR-positive parallel samples taken from the same patient at a same time. We also tested whether subtype prevalence differs in skin and blood samples...
  23. doi request reprint Sequencing-based molecular typing of treponema pallidum strains in the Czech Republic: all identified genotypes are related to the sequence of the SS14 strain
    Magdalena Flasarová
    Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, 625 00 Brno, Czech Republic
    Acta Derm Venereol 92:669-74. 2012
    ..The variants of arp and tpr genes appear to combine independently with sequence variants of TP0136, TP0548 and 23S rRNA genes...
  24. pmc Systematic cloning of Treponema pallidum open reading frames for protein expression and antigen discovery
    Matthew McKevitt
    Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030, USA
    Genome Res 13:1665-74. 2003
    ..pallidum antigens, and the remaining five represent novel antigens. These results demonstrate the potential of the T. pallidum clone set for antigen discovery and, more generally, for advancing the biology of this enigmatic spirochete...
  25. pmc BAC library of T. pallidum DNA in E. coli
    David Smajs
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston, TX 77030, USA
    Genome Res 12:515-22. 2002
    ..Using this minimal set of clones, at least 12 T. pallidum proteins were shown to react with pooled sera from rabbits immunized with T. pallidum, indicating that at least some T. pallidum genes are transcribed and expressed in E. coli...
  26. ncbi request reprint Occurrence of strains producing specific antibacterial inhibitory agents in five genera of Enterobacteriaceae
    Jan Smarda
    Department of Biology, Faculty of Medicine, Masaryk University, Tomesova 12, 602 00, Brno, Czech Republic
    Curr Microbiol 54:113-8. 2007
    ..The siderophore activity spectrum was rather wide in any genus, but the siderophores were usually not produced by strains producing phages or colicins...
  27. ncbi request reprint Construction of small genome BAC library for functional and genomic applications
    David Smajs
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, USA
    Methods Mol Biol 255:47-56. 2004
  28. pmc Unique Activity Spectrum of Colicin FY: All 110 Characterized Yersinia enterocolitica Isolates Were Colicin FY Susceptible
    Juraj Bosák
    Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
    PLoS ONE 8:e81829. 2013
    ..The universal susceptibility of Y. enterocolitica strains to colicin FY together with the absence of activity towards strains outside the Yersinia genus suggests potential therapeutic applications for colicin FY. ..
  29. doi request reprint Human Escherichia coli strains of different geographical and time source: bacteriocin types and their gene sequences are population-specific
    David Smajs
    Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic Departamento de Biologia Geral, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
    Environ Microbiol Rep 4:459-66. 2012
    ....
  30. pmc Detection of Treponema pallidum subsp. pallidum from skin lesions, serum, and cerebrospinal fluid in an infant with congenital syphilis after clindamycin treatment of the mother during pregnancy
    Vladana Woznicová
    Department of Medical Microbiology, Faculty of Medicine, Masaryk University, Pekarska 53, 656 91 Brno, Czech Republic
    J Clin Microbiol 45:659-61. 2007
    ..Using PCR detection of tmpC (TP0319) and DNA sequencing of the genes TP0136 and TP0548, DNA sequences identical to Treponema pallidum subsp. pallidum strain SS14 were detected in the infant's skin lesions, serum, and cerebrospinal fluid...
  31. ncbi request reprint Three recently acknowledged Escherichia species strikingly differ in the incidence of bacteriocinogenic and lysogenic strains
    Jan Smarda
    Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
    J Basic Microbiol 42:429-33. 2002
    ..From the 6 bacteriocinogenic strains of E. fergusonii, 3 were producers of colicin E1, 2 of colicin Ib and 1 of colicin Ia. In addition, 3 E. fergusonii strains produced aerobactin...
  32. ncbi request reprint Colicins produced by the Escherichia fergusonii strains closely resemble colicins encoded by Escherichia coli
    David Smajs
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston, TX, USA
    FEMS Microbiol Lett 208:259-62. 2002
    ..fergusonii strain EF36 (pColE1-EF36) was found to be only partially immune to E1 colicins produced by two other E. fergusonii strains suggesting that pColE1-EF36 may represent an ancestor ColE1 plasmid...
  33. ncbi request reprint Syphilis-causing strains belong to separate SS14-like or Nichols-like groups as defined by multilocus analysis of 19 Treponema pallidum strains
    Lukáš Nechvátal
    Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
    Int J Med Microbiol 304:645-53. 2014
    ....
  34. ncbi request reprint S-layers on cell walls of cyanobacteria
    Jan Smarda
    Department of Biology, Faculty of Medicine, Masaryk University, Jostova 10, CZ 662 44, Brno, Czech Republic
    Micron 33:257-77. 2002
    ..This cyanobacterium induces mineralization of fine-grain gypsum and calcite in a saturated lake fresh water solution. This process is involved in the formation of stromatolites...
  35. pmc Comparison of the genome of the oral pathogen Treponema denticola with other spirochete genomes
    Rekha Seshadri
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Proc Natl Acad Sci U S A 101:5646-51. 2004
    ....