Lumir Krejci

Summary

Affiliation: Masaryk University
Country: Czech Republic

Publications

  1. Samadder P, Suchankova T, Hylse O, Khirsariya P, Nikulenkov F, Drápela S, et al. Synthesis and Profiling of a Novel Potent Selective Inhibitor of CHK1 Kinase Possessing Unusual N-trifluoromethylpyrazole Pharmacophore Resistant to Metabolic N-dealkylation. Mol Cancer Ther. 2017;16:1831-1842 pubmed publisher
    ..Overall, MU380 represents a novel state-of-the-art CHK1 inhibitor with high potency, selectivity, and improved metabolic robustness to oxidative N-dealkylation. Mol Cancer Ther; 16(9); 1831-42. ©2017 AACR. ..
  2. Sedlackova H, Cechova B, Mlcousková J, Krejci L. RECQ4 selectively recognizes Holliday junctions. DNA Repair (Amst). 2015;30:80-9 pubmed publisher
    ..This study demonstrates biochemical activities of RECQ4 that could be involved in genome maintenance and suggest its possible role in processing replication and recombination intermediates. ..
  3. Lyakhovich A, Graifer D, Stefanovie B, Krejci L. Mitochondrial dysfunction in DDR-related cancer predisposition syndromes. Biochim Biophys Acta. 2016;1865:184-9 pubmed publisher
    ..Here, we will focus on the dual role of these syndromes in cancer predisposition and MDF with specific emphasis on impaired autophagy. ..
  4. Samadder P, Aithal R, Belan O, Krejci L. Cancer TARGETases: DSB repair as a pharmacological target. Pharmacol Ther. 2016;161:111-131 pubmed publisher
    ..Here we review the various alternative approaches to target the various protein classes termed as cancer TARGETases in DSB repair pathway to obtain more beneficial and selective therapy. ..
  5. Chavdarova M, Marini V, Sisakova A, Sedlackova H, Vigasová D, Brill S, et al. Srs2 promotes Mus81-Mms4-mediated resolution of recombination intermediates. Nucleic Acids Res. 2015;43:3626-42 pubmed publisher
    ..Concomitantly, Mus81-Mms4 inhibits the helicase activity of Srs2. Taken together, our data point to a coordinated role of Mus81-Mms4 and Srs2 in processing of recombination as well as replication intermediates. ..
  6. Kolinjivadi A, Sannino V, De Antoni A, Zadorozhny K, Kilkenny M, Técher H, et al. Smarcal1-Mediated Fork Reversal Triggers Mre11-Dependent Degradation of Nascent DNA in the Absence of Brca2 and Stable Rad51 Nucleofilaments. Mol Cell. 2017;67:867-881.e7 pubmed publisher
    ..These results indicate that Brca2 and Rad51 prevent formation of abnormal DNA replication intermediates, whose processing by Smarcal1 and Mre11 predisposes to genome instability. ..
  7. Lenart P, Krejci L. Reprint of "DNA, the central molecule of aging". Mutat Res. 2016;788:25-31 pubmed publisher
    ..Here, we review the roles of chromatin structure, DNA damage, and shortening of telomeres in aging and propose a hypothesis for how their interplay leads to aging phenotypes. ..
  8. Lenart P, Krejci L. DNA, the central molecule of aging. Mutat Res. 2016;786:1-7 pubmed publisher
    ..Here, we review the roles of chromatin structure, DNA damage, and shortening of telomeres in aging and propose a hypothesis for how their interplay leads to aging phenotypes. ..
  9. Kolesar P, Altmannova V, Silva S, Lisby M, Krejci L. Pro-recombination Role of Srs2 Protein Requires SUMO (Small Ubiquitin-like Modifier) but Is Independent of PCNA (Proliferating Cell Nuclear Antigen) Interaction. J Biol Chem. 2016;291:7594-607 pubmed publisher
    ..Notably, sumoylation of Srs2 itself specifically stimulates recombination at the rDNA locus. ..

More Information

Publications17

  1. Burkovics P, Dome L, Juhasz S, Altmannova V, Sebesta M, Pacesa M, et al. The PCNA-associated protein PARI negatively regulates homologous recombination via the inhibition of DNA repair synthesis. Nucleic Acids Res. 2016;44:3176-89 pubmed publisher
    ..Our data reveal a novel human regulatory mechanism that limits the extent of HR and represents a new potential target for anticancer therapy. ..
  2. Sebesta M, Urulangodi M, Stefanovie B, Szakal B, Pacesa M, Lisby M, et al. Esc2 promotes Mus81 complex-activity via its SUMO-like and DNA binding domains. Nucleic Acids Res. 2017;45:215-230 pubmed publisher
  3. Di Marco S, Hasanova Z, Kanagaraj R, Chappidi N, Altmannova V, Menon S, et al. RECQ5 Helicase Cooperates with MUS81 Endonuclease in Processing Stalled Replication Forks at Common Fragile Sites during Mitosis. Mol Cell. 2017;66:658-671.e8 pubmed publisher
    ..These data suggest that RECQ5 removes RAD51 filaments stabilizing stalled replication forks at CFSs and hence facilitates CFS cleavage by MUS81-EME1. ..
  4. Taylor M, Špírek M, Chaurasiya K, Ward J, Carzaniga R, Yu X, et al. Rad51 Paralogs Remodel Pre-synaptic Rad51 Filaments to Stimulate Homologous Recombination. Cell. 2015;162:271-286 pubmed publisher
    ..We propose that Rad51 paralogs stimulate HR by remodeling the Rad51 filament, priming it for strand exchange with the template duplex. ..
  5. Sarangi P, Altmannova V, Holland C, Bartosova Z, Hao F, Anrather D, et al. A versatile scaffold contributes to damage survival via sumoylation and nuclease interactions. Cell Rep. 2014;9:143-52 pubmed publisher
    ..These effects of Saw1 and its sumoylation suggest that Saw1 is a multifunctional scaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions. ..
  6. Zadorozhny K, Sannino V, Belan O, Mlcousková J, Spirek M, Costanzo V, et al. Fanconi-Anemia-Associated Mutations Destabilize RAD51 Filaments and Impair Replication Fork Protection. Cell Rep. 2017;21:333-340 pubmed publisher
    ..Our results further interconnect the FA pathway with DNA replication and provide mechanistic insight into the role of RAD51 in recombination-independent mechanisms of genome maintenance. ..
  7. Taylor M, Spirek M, Jian Ma C, Carzaniga R, Takaki T, Collinson L, et al. A Polar and Nucleotide-Dependent Mechanism of Action for RAD51 Paralogs in RAD51 Filament Remodeling. Mol Cell. 2016;64:926-939 pubmed publisher
    ..Finally, we discover that filament capping and stabilization are dependent on nucleotide binding, but not hydrolysis by RFS-1/RIP-1. These data define the mechanism of RAD51 filament remodeling by RAD51 paralogs. ..
  8. Sisakova A, Altmannova V, Sebesta M, Krejci L. Role of PCNA and RFC in promoting Mus81-complex activity. BMC Biol. 2017;15:90 pubmed publisher