Affiliation: Institute of Molecular Genetics
Country: Czech Republic
- Svoboda P. Cloning a transgene for transgenic RNAi in mouse oocytes. Cold Spring Harb Protoc. 2009;2009:pdb.prot5134 pubmed publisher..Subsequently, the IR is cloned, inserted into the transgenic cassette, and characterized by sequencing. Finally, the transgene is released from the cassette, purified, and provided to the transgenic facility. ..
- Flemr M, Moravec M, Libova V, Sedlacek R, Svoboda P. Lin28a is dormant, functional, and dispensable during mouse oocyte-to-embryo transition. Biol Reprod. 2014;90:131 pubmed publisher..Our data imply dual repression of Let-7 during OET in the mouse model, the selective suppression of Let-7 biogenesis by Lin28 homologs superimposed on previously reported global suppression of miRNA activity. ..
- Svoboda P. Long and small noncoding RNAs during oocyte-to-embryo transition in mammals. Biochem Soc Trans. 2017;45:1117-1124 pubmed publisher..Since some lncRNAs serve as precursors for small noncoding regulatory RNAs in RNA silencing pathways, both types of noncoding RNA are reviewed together. ..
- Svoboda P. Mammalian zygotic genome activation. Semin Cell Dev Biol. 2018;84:118-126 pubmed publisher..This review will discuss selected aspects of mammalian ZGA, highlighting shared features and evolved differences observed in commonly investigated mammals and non-mammalian model animals. ..