Petr Svoboda


Affiliation: Institute of Molecular Genetics
Country: Czech Republic


  1. Abe K, Yamamoto R, Franke V, Cao M, Suzuki Y, Suzuki M, et al. The first murine zygotic transcription is promiscuous and uncoupled from splicing and 3' processing. EMBO J. 2015;34:1523-37 pubmed publisher
    ..The results also suggest that the first zygotic transcription itself is an active component of chromatin remodeling in 1-cell embryos. ..
  2. Demeter T, Vaskovicova M, Malik R, Horvat F, Pasulka J, Svobodova E, et al. Main constraints for RNAi induced by expressed long dsRNA in mouse cells. Life Sci Alliance. 2019;2: pubmed publisher
    ..Although optimized endogenous Dicer substrates mimicking miRNA features could evolve for endogenous regulations, the same principles would make antiviral RNAi inefficient as viruses would adapt to avoid efficacy. ..
  3. Svoboda P. A toolbox for miRNA analysis. FEBS Lett. 2015;589:1694-701 pubmed publisher
    ..Accordingly, this review provides an elementary roadmap to navigate among available tools for miRNA analysis. The most common problems and errors observed in miRNA research are also discussed. ..
  4. Svoboda P, Franke V, Schultz R. Sculpting the Transcriptome During the Oocyte-to-Embryo Transition in Mouse. Curr Top Dev Biol. 2015;113:305-49 pubmed publisher
  5. Ganesh S, Svoboda P. Retrotransposon-associated long non-coding RNAs in mice and men. Pflugers Arch. 2016;468:1049-60 pubmed publisher
    ..In addition to summarizing essential features of mobile elements and lncRNAs, we focus on how retrotransposons contribute to lncRNA evolution, structure, and function in mammals. ..
  6. Svoboda P, Flemr M. The role of miRNAs and endogenous siRNAs in maternal-to-zygotic reprogramming and the establishment of pluripotency. EMBO Rep. 2010;11:590-7 pubmed publisher
  7. Svobodova E, Kubikova J, Svoboda P. Production of small RNAs by mammalian Dicer. Pflugers Arch. 2016;468:1089-102 pubmed publisher
    ..Its functionality differs between mammals and invertebrates: the mammalian Dicer is well adapted to produce miRNAs while its ability to support RNAi is limited. ..
  8. request reprint
    Svoboda P, Fulka H, Malik R. Clearance of Parental Products. Adv Exp Med Biol. 2017;953:489-535 pubmed
  9. Svoboda P. Cloning and sequencing an inverted repeat. Cold Spring Harb Protoc. 2009;2009:pdb.ip64 pubmed publisher
    ..Although we have successfully sequenced IRs using both strategies, we suggest using the latter to sequence transgenic constructs. ..

More Information


  1. Svoboda P. Cloning a transgene for transgenic RNAi in mouse oocytes. Cold Spring Harb Protoc. 2009;2009:pdb.prot5134 pubmed publisher
    ..Subsequently, the IR is cloned, inserted into the transgenic cassette, and characterized by sequencing. Finally, the transgene is released from the cassette, purified, and provided to the transgenic facility. ..
  2. Flemr M, Moravec M, Libova V, Sedlacek R, Svoboda P. Lin28a is dormant, functional, and dispensable during mouse oocyte-to-embryo transition. Biol Reprod. 2014;90:131 pubmed publisher
    ..Our data imply dual repression of Let-7 during OET in the mouse model, the selective suppression of Let-7 biogenesis by Lin28 homologs superimposed on previously reported global suppression of miRNA activity. ..
  3. Svoboda P. Long and small noncoding RNAs during oocyte-to-embryo transition in mammals. Biochem Soc Trans. 2017;45:1117-1124 pubmed publisher
    ..Since some lncRNAs serve as precursors for small noncoding regulatory RNAs in RNA silencing pathways, both types of noncoding RNA are reviewed together. ..
  4. Svoboda P. Mammalian zygotic genome activation. Semin Cell Dev Biol. 2018;84:118-126 pubmed publisher
    ..This review will discuss selected aspects of mammalian ZGA, highlighting shared features and evolved differences observed in commonly investigated mammals and non-mammalian model animals. ..