Affiliation: Institute of Molecular Genetics
Country: Czech Republic
Abe K, Yamamoto R, Franke V, Cao M, Suzuki Y, Suzuki M, et al
. The first murine zygotic transcription is promiscuous and uncoupled from splicing and 3' processing. EMBO J. 2015;34:1523-37 pubmed publisher
..The results also suggest that the first zygotic transcription itself is an active component of chromatin remodeling in 1-cell embryos. ..
Svoboda P. A toolbox for miRNA analysis. FEBS Lett. 2015;589:1694-701 pubmed publisher
..Accordingly, this review provides an elementary roadmap to navigate among available tools for miRNA analysis. The most common problems and errors observed in miRNA research are also discussed. ..
Svoboda P, Franke V, Schultz R. Sculpting the Transcriptome During the Oocyte-to-Embryo Transition in Mouse. Curr Top Dev Biol. 2015;113:305-49 pubmed publisher
Ganesh S, Svoboda P. Retrotransposon-associated long non-coding RNAs in mice and men. Pflugers Arch. 2016;468:1049-60 pubmed publisher
..In addition to summarizing essential features of mobile elements and lncRNAs, we focus on how retrotransposons contribute to lncRNA evolution, structure, and function in mammals. ..
Svoboda P, Flemr M. The role of miRNAs and endogenous siRNAs in maternal-to-zygotic reprogramming and the establishment of pluripotency. EMBO Rep. 2010;11:590-7 pubmed publisher
Svobodova E, Kubikova J, Svoboda P. Production of small RNAs by mammalian Dicer. Pflugers Arch. 2016;468:1089-102 pubmed publisher
..Its functionality differs between mammals and invertebrates: the mammalian Dicer is well adapted to produce miRNAs while its ability to support RNAi is limited. ..
Svoboda P, Fulka H, Malik R. Clearance of Parental Products. Adv Exp Med Biol. 2017;953:489-535 pubmed
Svoboda P. Cloning and sequencing an inverted repeat. Cold Spring Harb Protoc. 2009;2009:pdb.ip64 pubmed publisher
..Although we have successfully sequenced IRs using both strategies, we suggest using the latter to sequence transgenic constructs. ..
Svoboda P. Cloning a transgene for transgenic RNAi in mouse oocytes. Cold Spring Harb Protoc. 2009;2009:pdb.prot5134 pubmed publisher
..Subsequently, the IR is cloned, inserted into the transgenic cassette, and characterized by sequencing. Finally, the transgene is released from the cassette, purified, and provided to the transgenic facility. ..