Karel Slais

Summary

Affiliation: Institute of Analytical Chemistry
Country: Czech Republic

Publications

  1. ncbi request reprint Capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate
    Marie Horka
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veveri 97, 61142 Brno, Czech Republic
    Anal Chem 78:8438-44. 2006
  2. doi request reprint Isoelectric focusing in continuously tapered fused silica capillary prepared by etching with supercritical water
    Karel Slais
    Institute of Analytical Chemistry of the ASCR, v v i, Brno, Czech Republic
    Anal Chem 85:4296-300. 2013
  3. ncbi request reprint Fluorescein-based pI markers for capillary isoelectric focusing with laser-induced fluorescence detection
    Karel Slais
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veveri 97, CZ 61142 Brno, Czech Republic
    Electrophoresis 23:1682-8. 2002
  4. doi request reprint Divergent flow isoelectric focusing
    Karel Slais
    Institute of Analytical Chemistry, Academy of Sciences, Brno, Czech Republic
    Electrophoresis 29:2451-7. 2008
  5. ncbi request reprint CE separation of proteins and yeasts dynamically modified by PEG pyrenebutanoate with fluorescence detection
    Marie Horka
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic
    Electrophoresis 28:2300-7. 2007
  6. ncbi request reprint Capillary isoelectric focusing of proteins and microorganisms in dynamically modified fused silica with UV detection
    Marie Horka
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veveri 97, 611 42 Brno, Czech Republic
    J Chromatogr B Analyt Technol Biomed Life Sci 841:152-9. 2006
  7. doi request reprint Rapid separation and identification of the subtypes of swine and equine influenza A viruses by electromigration techniques with UV and fluorometric detection
    Marie Horka
    Institute of Analytical Chemistry of the ASCR, v v i, Veveri 97, 602 00 Brno, Czech Republic
    Analyst 136:3010-5. 2011
  8. doi request reprint Free flow and capillary isoelectric focusing of bacteria from the tomatoes plant tissues
    Marie Horka
    Institute of Analytical Chemistry of the ASCR, v v i, Veveri 97, 602 00 Brno, Czech Republic
    J Chromatogr A 1216:1019-24. 2009
  9. doi request reprint Capillary electromigration separation of proteins and microorganisms dynamically modified by chromophoric nonionogenic surfactant
    Marie Horka
    Institute of Analytical Chemistry Academy of Sciences of the Czech Republic, v v i, 602 00 Brno, Czech Republic
    Anal Chem 81:6897-904. 2009
  10. doi request reprint Dynamic labeling of diagnostically significant microbial cells in cerebrospinal fluid by red chromophoric non-ionogenic surfactant for capillary electrophoresis separations
    Marie Horka
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, vvi, Brno, Czech Republic
    Anal Chim Acta 728:86-92. 2012

Detail Information

Publications39

  1. ncbi request reprint Capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate
    Marie Horka
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veveri 97, 61142 Brno, Czech Republic
    Anal Chem 78:8438-44. 2006
    ..Using UV excitation for the on-column fluorometric detection, the minimum detectable amount was down to 10 cells injected on the separation capillary...
  2. doi request reprint Isoelectric focusing in continuously tapered fused silica capillary prepared by etching with supercritical water
    Karel Slais
    Institute of Analytical Chemistry of the ASCR, v v i, Brno, Czech Republic
    Anal Chem 85:4296-300. 2013
    ..The method described opens the way to increase the number of separable compounds without resorting to excessively high voltage...
  3. ncbi request reprint Fluorescein-based pI markers for capillary isoelectric focusing with laser-induced fluorescence detection
    Karel Slais
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veveri 97, CZ 61142 Brno, Czech Republic
    Electrophoresis 23:1682-8. 2002
    ..The examples of CIEF with fluorometric detection of new compounds together with fluorescein isothiocyanate (FITC) derivatized proteins are presented...
  4. doi request reprint Divergent flow isoelectric focusing
    Karel Slais
    Institute of Analytical Chemistry, Academy of Sciences, Brno, Czech Republic
    Electrophoresis 29:2451-7. 2008
    ..The developed divergent-flow IEF combines the speed of micro flow channels with the separation efficiency and sample load capacity of preparative devices...
  5. ncbi request reprint CE separation of proteins and yeasts dynamically modified by PEG pyrenebutanoate with fluorescence detection
    Marie Horka
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic
    Electrophoresis 28:2300-7. 2007
    ..Using UV excitation for the on-column fluorometric detection, the minimum detectable amounts of analytes, femtograms of proteins and down to ten cells injected on the separation capillary, were estimated...
  6. ncbi request reprint Capillary isoelectric focusing of proteins and microorganisms in dynamically modified fused silica with UV detection
    Marie Horka
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veveri 97, 611 42 Brno, Czech Republic
    J Chromatogr B Analyt Technol Biomed Life Sci 841:152-9. 2006
    ..The minimum detectable number of microbial cells was 5x10(2) to 1x10(3) with on-column UV detection at 280 nm...
  7. doi request reprint Rapid separation and identification of the subtypes of swine and equine influenza A viruses by electromigration techniques with UV and fluorometric detection
    Marie Horka
    Institute of Analytical Chemistry of the ASCR, v v i, Veveri 97, 602 00 Brno, Czech Republic
    Analyst 136:3010-5. 2011
    ..According to our experiments these methods appear to be useful for the fast preliminary differentiation of influenza viruses in future...
  8. doi request reprint Free flow and capillary isoelectric focusing of bacteria from the tomatoes plant tissues
    Marie Horka
    Institute of Analytical Chemistry of the ASCR, v v i, Veveri 97, 602 00 Brno, Czech Republic
    J Chromatogr A 1216:1019-24. 2009
    ..The microorganisms were of different origin, native and/or dynamically modified by the non-ionogenic tenside...
  9. doi request reprint Capillary electromigration separation of proteins and microorganisms dynamically modified by chromophoric nonionogenic surfactant
    Marie Horka
    Institute of Analytical Chemistry Academy of Sciences of the Czech Republic, v v i, 602 00 Brno, Czech Republic
    Anal Chem 81:6897-904. 2009
    ..At the same time, it has enabled the determination of albumin in human urine as a major clinical marker of urinary tract infections and kidney diseases...
  10. doi request reprint Dynamic labeling of diagnostically significant microbial cells in cerebrospinal fluid by red chromophoric non-ionogenic surfactant for capillary electrophoresis separations
    Marie Horka
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, vvi, Brno, Czech Republic
    Anal Chim Acta 728:86-92. 2012
    ....
  11. doi request reprint Candida "psilosis"--electromigration techniques and MALDI-TOF mass spectrometry for phenotypical discrimination
    Anna Kubesova
    Institute of Analytical Chemistry of the ASCR, v v i, Veveri 97, 602 00 Brno, Czech Republic
    Analyst 137:1937-43. 2012
    ....
  12. doi request reprint Combination of capillary isoelectric focusing in a tapered capillary with MALDI-TOF MS for rapid and reliable identification of Dickeya species from plant samples
    Marie Horka
    Institute of Analytical Chemistry of the ASCR, vvi, Brno, Czech Republic
    Anal Chem 85:6806-12. 2013
    ..It was found that the presence of plant tissue did not affect the results, making the proposed procedure very promising with respect to the fast and reliable detection and identification of bacteria in plant-tissue-containing samples. ..
  13. doi request reprint Capillary and gel electromigration techniques and MALDI-TOF MS--suitable tools for identification of filamentous fungi
    Marie Horka
    Institute of Analytical Chemistry of the ASCR, v v i, Brno, Czech Republic
    Anal Chim Acta 716:155-62. 2012
    ..On the other hand, the host origin of the fungi can play an important role in the precise fungi identification by the other techniques...
  14. ncbi request reprint Capillary isoelectric focusing of microorganisms in the pH range 2-5 in a dynamically modified FS capillary with UV detection
    Marie Horka
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veveri 97, 61142, Brno, Czech Republic
    Anal Bioanal Chem 385:840-6. 2006
    ..This CIEF method enables the separation and detection of the microbes from the mixed cultures within several minutes. The minimum detectable number of microbial cells was less than 10(3)...
  15. doi request reprint Divergent flow isoelectric focusing: fast and efficient method for protein sample preparation for mass spectrometry
    Karel Mazanec
    Institute of Analytical Chemistry of the ASCR, v v i, Veveri 97, 602 00, Brno, Czech Republic
    Anal Bioanal Chem 393:1769-78. 2009
    ..Results have shown the real potential of the suggested DF IEF device lay-out as an efficient preparative tool for separation and purification of complex protein mixtures for further analyses...
  16. ncbi request reprint Separation of plant pathogens from different hosts and tissues by capillary electromigration techniques
    Marie Horka
    Institute of Analytical Chemistry, v v i, Academy of Sciences of the Czech Republic, Veveri 97, 602 00 Brno, Czech Republic
    Anal Chem 79:9539-46. 2007
    ..Capillary electromigration was successfully applied for the separation of microbes directly from plant tissue suspensions...
  17. doi request reprint Capillary isoelectric focusing of probiotic bacteria from cow's milk in tapered fused silica capillary with off-line matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification
    Marie Horka
    Institute of Analytical Chemistry of the ASCR, vvi, Brno, Czech Republic
    Anal Chim Acta 788:193-9. 2013
    ..Our results have revealed that the proposed procedure can be advantageously used for unambiguous identification of probiotic bacteria in a real sample...
  18. ncbi request reprint Novel staining-free proteomic method for simultaneous identification of proteins and determination of their pI values by using low-molecular-mass pI markers
    Josef Chmelik
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic
    Electrophoresis 28:3315-23. 2007
    ..This method can be very useful for identification of proteins and their post-translational modifications in prefractioned samples, where post-translational modifications (e.g., glycation) are frequent...
  19. ncbi request reprint Dynamic modification of microorganisms by pyrenebutanoate for fluorometric detection in capillary zone electrophoresis
    Marie Horka
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno
    Electrophoresis 26:548-55. 2005
    ..Using UV excitation for on-column fluorometric detection, a detection sensitivity for the microorganisms on the order of from one to tens of injected cells was achieved...
  20. doi request reprint Separation of phenotypically indistinguishable Candida species, C. orthopsilosis, C. metapsilosis and C. parapsilosis, by capillary electromigration techniques
    Marie Horka
    Institute of Analytical Chemistry Academy of Sciences of the Czech Republic, vvi, Brno, Czech Republic
    J Chromatogr A 1218:3900-7. 2011
    ..Capillary isoelectric focusing was examined also for the separation and detection of the cultivated biofilm-negative C. parapsilosis in the blood serum...
  21. doi request reprint Separation of similar yeast strains by IEF techniques
    Marie Horka
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic
    Electrophoresis 30:2134-41. 2009
    ..Simultaneously, a fast and a simple technique was developed for the lysis of the outer membrane cell and characteristic fingerprints were found in lysate electrophoreograms and in gels from the capillary or the gel IEF, respectively...
  22. doi request reprint Electromigration techniques--a fast and economical tool for differentiation of similar strains of microorganisms
    Marie Horka
    Institute of Analytical Chemistry of the ASCR, v v i, Veveri 97, 602 00 Brno, Czech Republic
    Analyst 135:1636-44. 2010
    ..According to our experiments appropriate combination of electromigration techniques appears to be useful for the fast and economical differentiation of unknown microorganisms...
  23. ncbi request reprint Sol-gel column technology for capillary isoelectric focusing of microorganisms and biopolymers with UV or fluorometric detection
    Marie Horka
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno
    Electrophoresis 24:1383-90. 2003
    ..The compatibility of sol-gel capillaries with sensitive fluorometric detection of fluorescent pI markers together with fluorescein labeled proteins is demonstrated...
  24. doi request reprint Divergent-flow isoelectric focusing for separation and preparative analysis of peptides
    Filip Duša
    Institute of Analytical Chemistry of Academy of Sciences of Czech Republic, v v i, Brno, Czech Republic
    Electrophoresis 33:1687-94. 2012
    ..The potential of the DF IEF device as an efficient tool for the preparative scale separations was demonstrated by the isolation of caseinomacropeptide (CMP) from a crude whey solution...
  25. doi request reprint The trace analysis of microorganisms in real samples by combination of a filtration microcartridge and capillary isoelectric focusing
    Marie Horka
    Institute of Analytical Chemistry of the ASCR, Brno, Czech Republic
    Anal Bioanal Chem 400:3133-40. 2011
    ..The efficiency of preconcentration and a preliminary identification was verified by the phenotyping technique after cultivation of the spores sampled from the apple surface...
  26. doi request reprint Separation of attogram terpenes by the capillary zone electrophoresis with fluorometric detection
    Anna Kubesova
    Institute of Analytical Chemistry of the ASCR, v v i, Veveri 97, 602 00 Brno, Czech Republic
    J Chromatogr A 1217:7288-92. 2010
    ..It enables this yeast to cause serious nosocomial infections. The sensitivity of this method was demonstrated on the separation of farnesol directly from the cultivation medium...
  27. doi request reprint Preparative divergent flow IEF without carrier ampholytes for separation of complex biological samples
    Miroslava Stastna
    Institute of Analytical Chemistry of the ASCR, Brno, Czech Republic
    Electrophoresis 31:433-9. 2010
    ..3 as detected by polyacrylamide gel IEF with CBB staining. During DF IEF, the selected protein bands have been concentrated up to 16.8-fold...
  28. doi request reprint Testing of the influenza virus purification by CIEF
    Marie Horka
    Institute of Analytical Chemistry of the ASCR, Brno, Czech Republic
    Electrophoresis 31:331-8. 2010
    ..The pIs of both influenza viruses were determined. Simultaneously, the CIEF was found to be a suitable method for the rapid testing of the efficiency of the virus purification...
  29. ncbi request reprint Two-dimensional gel isoelectric focusing
    Miroslava Stastna
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic
    Electrophoresis 26:3586-91. 2005
    ..g., mass spectrometry analysis. The focusing properties of IEF could be useful especially in separation of mixtures, where components are at low concentration levels...
  30. doi request reprint Single-input divergent flow IEF for preparative analysis of proteins
    Miroslava Stastna
    Institute of Analytical Chemistry of the ASCR, Brno, Czech Republic
    Electrophoresis 29:4503-7. 2008
    ..26 to 3.96% of the bed output width...
  31. ncbi request reprint Colored pI standards and gel isoelectric focusing in strongly acidic pH
    Miroslava Stastna
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veveri 97, 611 42, Brno, Czech Republic
    Anal Bioanal Chem 382:65-72. 2005
    ..By using an experimental arrangement without electrode electrolyte reservoirs with electrodes creating the fixed end of the gel, the strongly acidic pH gradient was stable even for prolonged focusing time...
  32. ncbi request reprint Miniaturized liquid core waveguide-based fluorimetric detection cell for capillary separation methods: application in CE of amino acids
    Vratislav Kostal
    Department of Analytical Chemistry, Faculty of Science, Palacky University, Olomouc, Czech Republic
    Electrophoresis 27:4658-65. 2006
    ..The comparison of plasma amino acid levels of individuals being in good health with those of patients with inherited metabolic disorders is also shown...
  33. ncbi request reprint Fluorescence detection system for capillary separations utilizing a liquid core waveguide with an optical fibre-coupled compact spectrometer
    Vratislav Kostal
    Department of Analytical Chemistry, Faculty of Science, Palacky University, tr Svobody 26, 771 46 Olomouc, Czech Republic
    J Chromatogr A 1081:36-41. 2005
    ..The ability of the system to acquire fluorescent spectra is also demonstrated...
  34. doi request reprint Capillary electrophoresis of conidia from cultivated microscopic filamentous fungi
    Marie Horka
    Institute of Analytical Chemistry Academy of Sciences of the Czech Republic, 602 00 Brno, Czech Republic
    Anal Chem 81:3997-4004. 2009
    ..The observed isoelectric points were compared with those obtained from the separation of the cultured clinical samples, and they were found to be not host-specific...
  35. ncbi request reprint New azo dyes as colored isoelectric point markers for isoelectric focusing in acidic pH region
    Miroslava Stastna
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic
    Electrophoresis 26:53-9. 2005
    ..Subjected to gel IEF they show narrow and well-observable zones of different colors. Finally, our work resulted in suggestion of a color ladder composed of pI markers covering the pH range from 1.5 to 4.7...
  36. ncbi request reprint Mass spectrometric characterization of low-molecular-mass color pI markers and their use for direct determination of pI value of proteins
    Karel Mazanec
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veveri 97, 602 00 Brno, Czech Republic
    J Mass Spectrom 41:1570-7. 2006
    ..This work has confirmed that our approach can give information about the correct pI values of particular proteins and shorten significantly the time of analysis...
  37. ncbi request reprint Pyrenebutanoate as a dynamic protein modifier for fluorometric detection in capillary zone electrophoresis
    Marie Horka
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic
    Electrophoresis 23:1090-5. 2002
    ..Using deuterium lamp UV excitation for the on-column fluorometric detection, minimum detectable amounts in the amol-range of the proteins sampled on the CZE capillary was achieved...
  38. doi request reprint New solution IEF device for micropreparative separation of peptides and proteins
    Filip Duša
    Institute of Analytical Chemistry of the Academy of Sciences of the Czech Republic, v v i, Brno, Czech Republic
    Electrophoresis 34:1519-25. 2013
    ..We confirmed that the method is capable to process directly few tenths of milliliters of raw samples including the salty ones...
  39. ncbi request reprint Dynamics of gel isoelectric focusing with ampholytic dyes monitored by camera in real-time
    Miroslava Stastna
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veveri 97, 611 42 Brno, Czech Republic
    J Chromatogr A 1008:193-203. 2003
    ..The obtained animated records enabled the observation of the entire real focusing run within fractions of a minute, which is useful both for the understanding and optimization of the focusing...