Karel Slais

..Using UV excitation for the on-column fluorometric detection, the minimum detectable amount was down to 10 cells injected on the separation capillary...

..The developed divergent-flow IEF combines the speed of micro flow channels with the separation efficiency and sample load capacity of preparative devices...

..The examples of CIEF with fluorometric detection of new compounds together with fluorescein isothiocyanate (FITC) derivatized proteins are presented...

..Using UV excitation for the on-column fluorometric detection, the minimum detectable amounts of analytes, femtograms of proteins and down to ten cells injected on the separation capillary, were estimated...

..The minimum detectable number of microbial cells was 5x10(2) to 1x10(3) with on-column UV detection at 280 nm...

..According to our experiments these methods appear to be useful for the fast preliminary differentiation of influenza viruses in future...

..The microorganisms were of different origin, native and/or dynamically modified by the non-ionogenic tenside...

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..On the other hand, the host origin of the fungi can play an important role in the precise fungi identification by the other techniques...

..Results have shown the real potential of the suggested DF IEF device lay-out as an efficient preparative tool for separation and purification of complex protein mixtures for further analyses...

..This CIEF method enables the separation and detection of the microbes from the mixed cultures within several minutes. The minimum detectable number of microbial cells was less than 10(3)...

..Capillary electromigration was successfully applied for the separation of microbes directly from plant tissue suspensions...

..Simultaneously, a fast and a simple technique was developed for the lysis of the outer membrane cell and characteristic fingerprints were found in lysate electrophoreograms and in gels from the capillary or the gel IEF, respectively...

..According to our experiments appropriate combination of electromigration techniques appears to be useful for the fast and economical differentiation of unknown microorganisms...

..This method can be very useful for identification of proteins and their post-translational modifications in prefractioned samples, where post-translational modifications (e.g., glycation) are frequent...

..Capillary isoelectric focusing was examined also for the separation and detection of the cultivated biofilm-negative C. parapsilosis in the blood serum...

..Using UV excitation for on-column fluorometric detection, a detection sensitivity for the microorganisms on the order of from one to tens of injected cells was achieved...

..It enables this yeast to cause serious nosocomial infections. The sensitivity of this method was demonstrated on the separation of farnesol directly from the cultivation medium...

..The efficiency of preconcentration and a preliminary identification was verified by the phenotyping technique after cultivation of the spores sampled from the apple surface...

..3 as detected by polyacrylamide gel IEF with CBB staining. During DF IEF, the selected protein bands have been concentrated up to 16.8-fold...

..By using an experimental arrangement without electrode electrolyte reservoirs with electrodes creating the fixed end of the gel, the strongly acidic pH gradient was stable even for prolonged focusing time...

..26 to 3.96% of the bed output width...

..g., mass spectrometry analysis. The focusing properties of IEF could be useful especially in separation of mixtures, where components are at low concentration levels...

..The compatibility of sol-gel capillaries with sensitive fluorometric detection of fluorescent pI markers together with fluorescein labeled proteins is demonstrated...

..The pIs of both influenza viruses were determined. Simultaneously, the CIEF was found to be a suitable method for the rapid testing of the efficiency of the virus purification...

..At the same time, it has enabled the determination of albumin in human urine as a major clinical marker of urinary tract infections and kidney diseases...

..The observed isoelectric points were compared with those obtained from the separation of the cultured clinical samples, and they were found to be not host-specific...

..The ability of the system to acquire fluorescent spectra is also demonstrated...

..The comparison of plasma amino acid levels of individuals being in good health with those of patients with inherited metabolic disorders is also shown...

..The potential of the DF IEF device as an efficient tool for the preparative scale separations was demonstrated by the isolation of caseinomacropeptide (CMP) from a crude whey solution...

..Subjected to gel IEF they show narrow and well-observable zones of different colors. Finally, our work resulted in suggestion of a color ladder composed of pI markers covering the pH range from 1.5 to 4.7...

..This work has confirmed that our approach can give information about the correct pI values of particular proteins and shorten significantly the time of analysis...

..Using deuterium lamp UV excitation for the on-column fluorometric detection, minimum detectable amounts in the amol-range of the proteins sampled on the CZE capillary was achieved...

..The obtained animated records enabled the observation of the entire real focusing run within fractions of a minute, which is useful both for the understanding and optimization of the focusing...