J Petrak

Summary

Affiliation: Institute of Hematology and Blood Transfusion
Country: Czech Republic

Publications

  1. ncbi request reprint Detection of iron-containing proteins contributing to the cellular labile iron pool by a native electrophoresis metal blotting technique
    J V Petrák
    Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20 2, Praha, Czech Republic
    J Inorg Biochem 86:669-75. 2001
  2. doi request reprint Déjà vu in proteomics. A hit parade of repeatedly identified differentially expressed proteins
    Jiri Petrak
    Institute of Hematology and Blood Transfusion, Prague, Czech Republic
    Proteomics 8:1744-9. 2008
  3. ncbi request reprint Iron-independent specific protein expression pattern in the liver of HFE-deficient mice
    Jiri Petrak
    Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20 Prague, Czech Republic
    Int J Biochem Cell Biol 39:1006-15. 2007
  4. ncbi request reprint Hephaestin--a ferroxidase of cellular iron export
    Jiri Petrak
    Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20 Praha 2, Czech Republic
    Int J Biochem Cell Biol 37:1173-8. 2005
  5. ncbi request reprint [Proteomics and its role in medicine]
    J Petrak
    Ustav hematologie a krevni transfuze, Praha
    Cas Lek Cesk 144:365-70; discussion 370-1. 2005
  6. ncbi request reprint Proteomic analysis of iron overload in human hepatoma cells
    Jiri Petrak
    Institute of Hematology and Blood Transfusion, U Nemocnice 1, Prague, Czech Republic
    Am J Physiol Gastrointest Liver Physiol 290:G1059-66. 2006
  7. ncbi request reprint Proteomic analysis of hepatic iron overload in mice suggests dysregulation of urea cycle, impairment of fatty acid oxidation, and changes in the methylation cycle
    Jiri Petrak
    Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20 Prague, Czech Republic
    Am J Physiol Gastrointest Liver Physiol 292:G1490-8. 2007
  8. ncbi request reprint Proteomic analysis of erythroid differentiation induced by hexamethylene bisacetamide in murine erythroleukemia cells
    Jiri Petrak
    Institute of Hematology and Blood Transfusion, Prague, Czech Republic
    Exp Hematol 35:193-202. 2007
  9. ncbi request reprint Iron transport in K562 cells: a kinetic study using native gel electrophoresis and 59Fe autoradiography
    D Vyoral
    Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20, Praha 2, Czech Republic
    Biochim Biophys Acta 1403:179-88. 1998
  10. ncbi request reprint Native proteomic analysis of protein complexes in murine intestinal brush border membranes
    Marek Babusiak
    Institute of Hematology and Blood Transfusion, Prague, Czech Republic
    Proteomics 7:121-9. 2007

Collaborators

  • D Vyoral
  • Marek Babusiak
  • Petr Halada
  • Prem Ponka
  • Jan Cerny
  • Des R Richardson
  • Radek Cmejla
  • Jana Cmejlova
  • Dagmar Pospisilova
  • Gabriela Peslova
  • Helena Handrkova
  • Kvetoslava Petrtylova
  • O Fuchs
  • Ondrej Sebesta
  • Filipp Savvulidi
  • Pavlina Ptackova
  • Ivan Hrdy
  • Erika Becker
  • Philip W Kuchel
  • Yahia Jabali
  • Michael Li Hsuan Huang
  • Vladimir Mihal
  • Zdena Cerna
  • Yohan Suryo Rahmanto
  • Shan Soe-Lin
  • Robert Sutak
  • Katerina Kuzelova
  • Jan Stary
  • Ludmila Dolezalova

Detail Information

Publications18

  1. ncbi request reprint Detection of iron-containing proteins contributing to the cellular labile iron pool by a native electrophoresis metal blotting technique
    J V Petrák
    Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20 2, Praha, Czech Republic
    J Inorg Biochem 86:669-75. 2001
    ..Using a new native metal blotting method, we have confirmed that specific high molecular weight proteins bind the labile iron pool...
  2. doi request reprint Déjà vu in proteomics. A hit parade of repeatedly identified differentially expressed proteins
    Jiri Petrak
    Institute of Hematology and Blood Transfusion, Prague, Czech Republic
    Proteomics 8:1744-9. 2008
    ..We consider if these commonly observed changes represent common cellular stress responses or are a reflection of the technical limitations of 2-DE...
  3. ncbi request reprint Iron-independent specific protein expression pattern in the liver of HFE-deficient mice
    Jiri Petrak
    Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20 Prague, Czech Republic
    Int J Biochem Cell Biol 39:1006-15. 2007
    ....
  4. ncbi request reprint Hephaestin--a ferroxidase of cellular iron export
    Jiri Petrak
    Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20 Praha 2, Czech Republic
    Int J Biochem Cell Biol 37:1173-8. 2005
    ..In addition to the small intestine, hephaestin is expressed to a lesser extent in colon, spleen, placenta and kidney but its role in these tissues remains unknown. So far, hephaestin has not been linked to a human disease...
  5. ncbi request reprint [Proteomics and its role in medicine]
    J Petrak
    Ustav hematologie a krevni transfuze, Praha
    Cas Lek Cesk 144:365-70; discussion 370-1. 2005
    ....
  6. ncbi request reprint Proteomic analysis of iron overload in human hepatoma cells
    Jiri Petrak
    Institute of Hematology and Blood Transfusion, U Nemocnice 1, Prague, Czech Republic
    Am J Physiol Gastrointest Liver Physiol 290:G1059-66. 2006
    ..Many of these molecules have not been previously suggested to be involved in the response to iron overload and the consequent oxidative stress...
  7. ncbi request reprint Proteomic analysis of hepatic iron overload in mice suggests dysregulation of urea cycle, impairment of fatty acid oxidation, and changes in the methylation cycle
    Jiri Petrak
    Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20 Prague, Czech Republic
    Am J Physiol Gastrointest Liver Physiol 292:G1490-8. 2007
    ..This pattern of changes likely reflects compensatory and pathological changes associated with liver iron overload and provides a window into these processes...
  8. ncbi request reprint Proteomic analysis of erythroid differentiation induced by hexamethylene bisacetamide in murine erythroleukemia cells
    Jiri Petrak
    Institute of Hematology and Blood Transfusion, Prague, Czech Republic
    Exp Hematol 35:193-202. 2007
    ..To deepen our understanding of the molecular mechanisms underlying and erythroid differentiation and leukemia we monitored changes in protein expression in differentiating MEL cells...
  9. ncbi request reprint Iron transport in K562 cells: a kinetic study using native gel electrophoresis and 59Fe autoradiography
    D Vyoral
    Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20, Praha 2, Czech Republic
    Biochim Biophys Acta 1403:179-88. 1998
    ..The nature of the labile iron pool and its susceptibility to iron chelation is discussed...
  10. ncbi request reprint Native proteomic analysis of protein complexes in murine intestinal brush border membranes
    Marek Babusiak
    Institute of Hematology and Blood Transfusion, Prague, Czech Republic
    Proteomics 7:121-9. 2007
    ..In addition, BN-PAGE technique preserves metal-protein interactions, as shown by the presence of 65Zn in metalloprotein complexes, isolated from zinc-radiolabeled BBMs...
  11. ncbi request reprint Translational efficiency in patients with Diamond-Blackfan anemia
    Jana Cmejlova
    Department of Cell Physiology, Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20, Prague, Czech Republic
    Haematologica 91:1456-64. 2006
    ..We attempted to elucidate the importance of RPS19 in translation in relation to the pathogenesis of DBA...
  12. ncbi request reprint Identification of heme binding protein complexes in murine erythroleukemic cells: study by a novel two-dimensional native separation -- liquid chromatography and electrophoresis
    Marek Babusiak
    Institute of Hematology and Blood Transfusion, Prague, Czech Republic
    Proteomics 5:340-50. 2005
    ..Thirty-three individual proteins were identified and attributed to four novel multiprotein complexes representing four different 'snapshots' of cellular events involved in hemoglobin biosynthesis...
  13. ncbi request reprint Role of zinc in eukaryotic cells, zinc transporters and zinc-containing proteins. Review article
    O Fuchs
    Institute of Haematology and Blood Transfusion, U Nemocnice 1, 128 20 Prague 2, Czech Republic
    Sb Lek 104:157-70. 2003
    ..The present explosive growth of new knowledge about various biological roles of zinc will undoubtedly lead to the future development of new powerful drugs and to treatment of many diseases including cancer...
  14. ncbi request reprint A novel iron responsive element in the 3'UTR of human MRCKalpha
    Radek Cmejla
    Institute of Hematology and Blood Transfusion, Department of Cell Physiology, U Nemocnice 1, Prague, Czech Republic
    Biochem Biophys Res Commun 341:158-66. 2006
    ..The level of MRCKalpha mRNA in various tissues strongly positively correlates with the level of TfR mRNA, indicating its possible role in the transferrin iron uptake pathway...
  15. ncbi request reprint Ribosomal protein S17 gene (RPS17) is mutated in Diamond-Blackfan anemia
    Radek Cmejla
    Department of Cell Physiology, Institute of Hematology and Blood Transfusion, Prague, Czech Republic
    Hum Mutat 28:1178-82. 2007
    ..The identification of a mutation in the third RP of the small ribosomal subunit in DBA patients further supports the theory that impaired translation may be the main cause of DBA pathogenesis...
  16. doi request reprint Identification of mutations in the ribosomal protein L5 (RPL5) and ribosomal protein L11 (RPL11) genes in Czech patients with Diamond-Blackfan anemia
    Radek Cmejla
    Department of Cell Physiology, Institute of Hematology and Blood Transfusion, Prague, Czech Republic
    Hum Mutat 30:321-7. 2009
    ..Since RPL5 and RPL11, together with RPL23, are also involved in the MDM2-mediated p53 pathway regulation, we also screened the RPL23 gene for mutations; however, no mutations were identified...
  17. doi request reprint Hepcidin, the hormone of iron metabolism, is bound specifically to alpha-2-macroglobulin in blood
    Gabriela Peslova
    Institute of Hematology and Blood Transfusion, Prague, Czech Republic
    Blood 113:6225-36. 2009
    ..The demonstration that alpha2-M is the hepcidin transporter could lead to better understanding of hepcidin physiology, methods for its sensitive measurement and the development of novel drugs for the treatment of iron-related diseases...
  18. doi request reprint Human MRCKalpha is regulated by cellular iron levels and interferes with transferrin iron uptake
    Radek Cmejla
    Institute of Hematology and Blood Transfusion, Department of Cell Physiology, U Nemocnice 1, Prague 128 20, Czech Republic
    Biochem Biophys Res Commun 395:163-7. 2010
    ..Regulation of the MRCKalpha activity by intracellular iron levels could thus represent another molecular feedback mechanism cells could use to finely tune iron uptake to actual needs...