Jiri Homola

..This paper reviews fundamentals of SPR affinity biosensors and discusses recent advances in development and applications of SPR biosensors...

..In addition, we report application of a recently developed multichannel SPR sensor based on spectroscopy of surface plasmons and wavelength division multiplexing of sensing channels to multi-analyte detection...

..We demonstrate a limit of detection of 100 pM and a surface density resolution of only 35 fg×mm-2 that corresponds to less than one DNA molecule per nanoparticle on average...

..Moreover, the limit of detection achieved using this approach is better by a factor of more than 1,000 than limits of detection reported so far for CEA in blood plasma using SPR biosensors...

..Moreover, it is demonstrated that the use of a buffer with the appropriate cation composition can improve the discrimination of complementary and point mismatched DNA targets...

..Detection of specific DNA or RNA sequences can be performed in less than 15 min by the reported SPR sensor...

..The assay is applied to detection of a short synthetic analogue of TP53 containing a "hot spot"-single nucleotide mismatch frequently mutated in germ line cancer-at levels down to 40 pM...

..This chapter reviews the main principles of SPR biosensor technology and discusses applications of this technology for rapid, sensitive and specific detection of chemical and biological analytes...

..In the model experiment, this approach is shown to enable direct detection of hCG in blood plasma at levels which are five times lower than those detected using the conventional detection approach...

..It is demonstrated that the SPRCD sensor is able to measure bulk refractive index changes as small as 3 x 10(-7) RIU (refractive index units) and to detect short oligonucleotides in concentrations down to 200 pM...

..A protein monolayer has been shown to produce a response of 5000 times the baseline noise, suggesting that as few as ~500 proteins could be detected by the sensor...

..A few hundred square micrometers large, continuous and fully defect-free areas of gold nanoparticles were produced on the nearly entire surface of the templated substrates...

..The methodology is evaluated for analysis of miRNA from mouse liver tissues and is found to yield results which agree well with those provided by the quantitative polymerase chain reaction (qPCR)...

..Limits of detection as low as 45 ng/mL (ALCAM) and 100 ng/mL (hCG) were achieved in blood plasma samples...

..It was demonstrated that the sensor is capable of detecting concentrations of anti-EBNA as low as 0.2 ng/ml (approximately 1 pM) both in buffer and 1% human serum and can be stored and regenerated for repeated use...

..The sensitivity of these dips to changes in the refractive index of the analyte is calculated. Moreover, the refractive index resolution of the sensor was estimated to be better than 2 x 10(-6) RIU...

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..The detection limit of 25 ng/mL is achieved. In addition, we demonstrate that the functional surface of the sensor can be regenerated for repeated use...

..The potential of the assay is demonstrated in the study in which the ability of natural and modified oligonucleotides to activate RNase H is examined...

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..We utilize these results to discuss the applicability of this scaling behavior with respect to a generalized affinity-based biosensor...

..It was demonstrated that the sensor is capable of detecting complementary 23-mer ONs in concentrations as low as 0.1 nM with high specificity and reproducibility...

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..The results indicate that the sensitivity is strongly correlated with the profile of the LSP electric field...

..We show that the sensor can be designed to have similar sensitivity and even better resolution compared with those for a conventional configuration...