Iva Dykova

..We present its detailed description here also to demonstrate that amoeba stages of this type of organisms are capable to infect fishes...

..In other organs of these three host species, xenoma-like formations (XLFs) were found that severely injured blood vessels. XLFs contained presporogonic stages of S. fegati, whose species identity was evidenced using molecular methods...

..Sequences obtained in this study have been deposited in GenBank with accession numbers DQ768714-DQ768743...

..This complexity needs to be considered when attempting to define molecular markers for identification of Paramoeba/Neoparamoeba species in tissues of fish and invertebrates...

..Data obtained in this study may be useful in future analyses of relationships of the genera Didymium, Hyperamoeba, Physarum and Pseudodidymium as well as in higher-order phylogeny of Myxogastrea...

..No. EU232760). Phylogenetic analysis of SSU rDNA revealed that S. fegati is closely related to myxosporeans infecting gall bladders of freshwater fish...

..Comparison of phylograms reconstructed from corresponding SSU rDNA sequences of host amoebae and their symbionts revealed a high level of congruence, which argues very strongly for coevolution of these two eukaryotic organisms...

..hilla verified the assumption that the extra- and intra-cellularly multiplying Labyrinthula cells are identical organisms. The association of the marine amoeba T. hilla and Labyrinthula sp. displayed signs of mutualistic symbiosis...

..were positioned within a sister-group containing Paraflabellula spp. Results of phylogenetic analysis confirmed doubts of previous authors regarding generic assignment of several Rhizanmoeba and Ripidomnyxa strains...

..inornata recognized in this study should motivate further screening for infections in its host, which is considered a commercially important game fish with a wide distribution in the Western North Atlantic...

..robusta is sensitive to the method of analysis applied, a tendency to group with Acramoeba dendroida Smirnov, Nassonova et Cavalier-Smith, 2008 is evident...

..On the basis of SSU rDNA, the 6th strain, also isolated from affected fish gills, was identified as Aristerostoma sp...

..with NGD and a direct ISH proof of Naegleria trophozoites attached to hyperplastic gill epithelium are novel findings, expanding the number of possible agents of NGD and supporting the hypothesis on multicausal aetiology of this disease...

..aff. antillarum mortality. The enlargement of the dataset of SSU rDNA sequences brought new insight into the phylogeny of Neoparamoeba species...

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..A new species-specific oligonucleotide probe inferred from recently available SSU rRNA gene sequences was designed and successfully tested in tissue lesions produced by one strain of H. vermiformis in experimentally infected fish...

..Members of this clade are characterized as representatives of a new species, N. branchiphila n. sp. The diagnostic primers for the identification of this species are introduced...

..The pathogenic potential of epizoic gill amoebae in turbots is discussed...

..Spore-laden macrophages were found in various tissues and organs; their infiltration in epidermis including its outermost layers may effectively enhance the spread of infection while the hosts still live...

..pemaquidensis, the closely related species N. aestuarina also has to be taken into consideration as the agent of AGD...

..australiensis. Another 5 strains had a 1.3 kb long group I intron in their SSU rRNA gene and, based on the SSU rRNA sequences and riboprints, RFLP-PCR patterns appeared in phylogenetic trees to be closely related to Naegleria clarki...

..dianae is distinguishable from these five species and that its closest relation is with K. miniauiriculata...

..The damage may result in complete disintegration of the plasmodium. However, the development of the microsporidian is affected by a remarkably high percentage of teratological stages revealing membranaceous and tubular structures...

..pemaquidensis Page, 1970. The identity with P. pemaquidensis, known as an agent of gill diseases of salmonids, is yet to be assessed using non-morphological criteria...

..Phylogenetic analyses elucidated relationship of the newly described Myxobolus lentisuturalis to other Myxobolus species and supported its position of an independent species...

..The bacterial endosymbiont found in N. pattersoni type strain RR2G2 is assigned to the genus Rickettsia...

..Phylogenetic analysis based on these sequences has allowed clearer phylogenetic relationships to be established with other species of the genus Myxobolus sequenced to date...

..Attention is particularly given to specific diagnostic tools for fish-infecting amphizoic amoebae and ways for their further development...

..Three newly sequenced species of the genus Chloromyxum represent three separate lineages within the myxosporean tree and do not support the monophyly of this genus...

..formed a monophyletic group with a sister-group relationship to Vannella anglica Page, 1980. They did not cluster with Gymnamoebae of the families Hartmannellidae, Flabellulidae, Leptomyxidae or Amoebidae presently available in GenBank...

..Establishment of a classification acceptable from morphological, biological and phylogenetical viewpoints is necessary...

..The need of further research is emphasised...

..Cephalic papillae of the external circle differ from those in other congeners in that the dorso-lateral and ventro-lateral papillae are large, dome-shaped, whereas the dorso-dorsal and ventro-ventral papillae are small...

..comb. This is the first record of this parasite from Peru, and P. perruno represents its new host record...

..obtusus from the closely related P. acutus Dujardin, 1845. The finding of the former in H. edwardsii represents a new host record...

..The microsporidium was observed in the abscess aspirate, and its specific DNA was also detected in stool and urine. The patient was successfully treated with albendazole and mebendazole...

..The results imply that tick-borne encephalitis is an immunopathological disease and that the inflammatory reaction significantly contributes to the fatal outcome of the infection...