Jaroslav Dolezel

Summary

Affiliation: Institute of Experimental Botany
Country: Czech Republic

Publications

  1. ncbi request reprint Chromosome sorting and PCR-based physical mapping in pea (Pisum sativum L.)
    Pavel Neumann
    Institute of Plant Molecular Biology, Ceske Budejovice, Czech Republic
    Chromosome Res 10:63-71. 2002
  2. ncbi request reprint Advanced resources for plant genomics: a BAC library specific for the short arm of wheat chromosome 1B
    Jaroslav Janda
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovska 6, CZ 77200 Olomouc, Czech Republic
    Plant J 47:977-86. 2006
  3. doi request reprint A physical map of the 1-gigabase bread wheat chromosome 3B
    Etienne Paux
    Institut National de la Recherche Agronomique, Université Blaise Pascal INRA UBP, UMR 1095, Genetics Diversity and Ecophysiology of Cereals, Clermont Ferrand, France
    Science 322:101-4. 2008
  4. ncbi request reprint Introduction: chromosomes go with the flow
    Jaroslav Dolezel
    Chromosome Res 12:1-4. 2004
  5. ncbi request reprint Flow cytogenetics and plant genome mapping
    Jaroslav Dolezel
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolorakrá 6, Olomouc, CZ 77200, Czech Republic
    Chromosome Res 12:77-91. 2004
  6. pmc Molecular analysis and genomic organization of major DNA satellites in banana (Musa spp.)
    Jana Čížková
    Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic
    PLoS ONE 8:e54808. 2013
  7. pmc A platform for efficient genotyping in Musa using microsatellite markers
    Pavla Christelová
    Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Sokolovska 6, Olomouc CZ 77200, Czech Republic
    AoB Plants 2011:plr024. 2011
  8. pmc The ITS1-5.8S-ITS2 sequence region in the Musaceae: structure, diversity and use in molecular phylogeny
    Eva Hřibová
    Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Olomouc, Czech Republic
    PLoS ONE 6:e17863. 2011
  9. pmc Chromosome isolation by flow sorting in Aegilops umbellulata and Ae. comosa and their allotetraploid hybrids Ae. biuncialis and Ae. geniculata
    Istvan Molnar
    Agricultural Research Institute of the Hungarian Academy of Sciences, Martonvasar, Hungary
    PLoS ONE 6:e27708. 2011
  10. doi request reprint Advances in plant chromosome genomics
    Jaroslav Dolezel
    Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Šlechtitelů 31, Olomouc CZ 78371, Czech Republic Electronic address
    Biotechnol Adv 32:122-36. 2014

Collaborators

Detail Information

Publications51

  1. ncbi request reprint Chromosome sorting and PCR-based physical mapping in pea (Pisum sativum L.)
    Pavel Neumann
    Institute of Plant Molecular Biology, Ceske Budejovice, Czech Republic
    Chromosome Res 10:63-71. 2002
    ..These experiments enabled assignment of the linkage groups IV and VII to chromosomes 4 and 7, respectively...
  2. ncbi request reprint Advanced resources for plant genomics: a BAC library specific for the short arm of wheat chromosome 1B
    Jaroslav Janda
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovska 6, CZ 77200 Olomouc, Czech Republic
    Plant J 47:977-86. 2006
    ....
  3. doi request reprint A physical map of the 1-gigabase bread wheat chromosome 3B
    Etienne Paux
    Institut National de la Recherche Agronomique, Université Blaise Pascal INRA UBP, UMR 1095, Genetics Diversity and Ecophysiology of Cereals, Clermont Ferrand, France
    Science 322:101-4. 2008
    ..This physical map establishes a template for the remaining wheat chromosomes and demonstrates the feasibility of constructing physical maps in large, complex, polyploid genomes with a chromosome-based approach...
  4. ncbi request reprint Introduction: chromosomes go with the flow
    Jaroslav Dolezel
    Chromosome Res 12:1-4. 2004
  5. ncbi request reprint Flow cytogenetics and plant genome mapping
    Jaroslav Dolezel
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolorakrá 6, Olomouc, CZ 77200, Czech Republic
    Chromosome Res 12:77-91. 2004
    ..This paper provides a comprehensive review of the methodology and application of flow cytogenetics, and assesses its potential for plant genome analysis...
  6. pmc Molecular analysis and genomic organization of major DNA satellites in banana (Musa spp.)
    Jana Čížková
    Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic
    PLoS ONE 8:e54808. 2013
    ..In addition to improving the knowledge on Musa satellite DNA, our study increases the number of cytogenetic markers and the number of individual chromosomes, which can be identified in Musa...
  7. pmc A platform for efficient genotyping in Musa using microsatellite markers
    Pavla Christelová
    Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Sokolovska 6, Olomouc CZ 77200, Czech Republic
    AoB Plants 2011:plr024. 2011
    ..With the aim of providing a simple and robust approach for molecular characterization of Musa species, we developed an optimized genotyping platform using 19 published simple sequence repeat markers...
  8. pmc The ITS1-5.8S-ITS2 sequence region in the Musaceae: structure, diversity and use in molecular phylogeny
    Eva Hřibová
    Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Olomouc, Czech Republic
    PLoS ONE 6:e17863. 2011
    ..The observation of only one type of ITS sequence in some of the presumed interspecific hybrid clones warrants further study to confirm their hybrid origin and to unravel processes leading to evolution of their genomes...
  9. pmc Chromosome isolation by flow sorting in Aegilops umbellulata and Ae. comosa and their allotetraploid hybrids Ae. biuncialis and Ae. geniculata
    Istvan Molnar
    Agricultural Research Institute of the Hungarian Academy of Sciences, Martonvasar, Hungary
    PLoS ONE 6:e27708. 2011
    ..The results obtained in this work provide new opportunities for dissecting genomes of wild relatives of wheat with the aim to assist in alien gene transfer and discovery of novel genes for wheat improvement...
  10. doi request reprint Advances in plant chromosome genomics
    Jaroslav Dolezel
    Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Šlechtitelů 31, Olomouc CZ 78371, Czech Republic Electronic address
    Biotechnol Adv 32:122-36. 2014
    ....
  11. pmc Chromosome arm-specific BAC end sequences permit comparative analysis of homoeologous chromosomes and genomes of polyploid wheat
    Sunish K Sehgal
    Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA
    BMC Plant Biol 12:64. 2012
    ..Here we report on the use of a BAC library made from flow-sorted telosomic chromosome 3A short arm (t3AS) for marker development and analysis of sequence composition and comparative evolution of homoeologous genomes of hexaploid wheat...
  12. pmc Integration of mate pair sequences to improve shotgun assemblies of flow-sorted chromosome arms of hexaploid wheat
    Tatiana Belova
    Department of Plant and Environmental Sciences, University of Life Sciences, As, Norway
    BMC Genomics 14:222. 2013
    ..The aim of this work was to assess how mate pair data generated from multiple displacement amplified DNA of flow-sorted chromosomes affect assembly fragmentation of shotgun assemblies of the wheat chromosomes...
  13. pmc Chromosomes in the flow to simplify genome analysis
    Jaroslav Dolezel
    Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Sokolovska 6, Olomouc, Czech Republic
    Funct Integr Genomics 12:397-416. 2012
    ..This review explains the principles of flow cytometric chromosome analysis and sorting (flow cytogenetics), discusses the major uses of this technology in genome analysis, and outlines future directions...
  14. pmc Plant centromeric retrotransposons: a structural and cytogenetic perspective
    Pavel Neumann
    Biology Centre of the Academy of Sciences of the Czech Republic, Institute of Plant Molecular Biology, Branisovska 31, České Budějovice CZ 37005, Czech Republic
    Mob DNA 2:4. 2011
    ..abstract:..
  15. pmc Structure and evolution of Apetala3, a sex-linked gene in Silene latifolia
    Radim Cegan
    Laboratory of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, V, V, I, Kralovopolska 135, CZ 612 65 Brno, Czech Republic
    BMC Plant Biol 10:180. 2010
    ..Recently, the gene AP3 was characterized in the dioecious plant species Silene latifolia. It was suggested that this gene had been transferred from an autosome to the Y chromosome...
  16. pmc Feasibility of physical map construction from fingerprinted bacterial artificial chromosome libraries of polyploid plant species
    Ming Cheng Luo
    Department of Plant Sciences, University of California, Davis, CA 95616, USA
    BMC Genomics 11:122. 2010
    ....
  17. ncbi request reprint Chromosome-based genomics in the cereals
    Jaroslav Dolezel
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovska 6, CZ 77200, Olomouc, Czech Republic
    Chromosome Res 15:51-66. 2007
    ....
  18. ncbi request reprint Estimation of nuclear DNA content in plants using flow cytometry
    Jaroslav Dolezel
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovska 6, Olomouc, Czech Republic
    Nat Protoc 2:2233-44. 2007
    ..The purpose and requirement of internal and external standardization are discussed. The importance of using a correct terminology for DNA amounts and genome size is underlined, and its basic principles are explained...
  19. ncbi request reprint Plant DNA flow cytometry and estimation of nuclear genome size
    Jaroslav Dolezel
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovska 6, Olomouc, CZ 77200, Czech Republic
    Ann Bot 95:99-110. 2005
    ..While the former applications are relatively straightforward, estimation of absolute DNA amount requires special attention to possible errors in sample preparation and analysis...
  20. doi request reprint Nuclear genome size: are we getting closer?
    Jaroslav Dolezel
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovska 6, CZ 77200 Olomouc, Czech Republic
    Cytometry A 77:635-42. 2010
    ..This article assesses the current state of DNA reference standards for flow cytometry and the issues related to their calibration...
  21. doi request reprint Chromosome analysis and sorting using flow cytometry
    Jaroslav Dolezel
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Olomouc, Czech Republic
    Methods Mol Biol 701:221-38. 2011
    ..Chromosomes isolated according to this protocol have well-preserved morphology, withstand shearing forces during sorting, and their DNA is intact and suitable for a range of applications...
  22. pmc Chromosome sorting in tetraploid wheat and its potential for genome analysis
    Marie Kubaláková
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Olomouc, Czech Republic
    Genetics 170:823-9. 2005
    ..This study considerably expands the potential of flow cytogenetics for use in wheat genomics and opens the possibility of sequencing the genome of this important crop one chromosome arm at a time...
  23. pmc A novel resource for genomics of Triticeae: BAC library specific for the short arm of rye (Secale cereale L.) chromosome 1R (1RS)
    Hana Simkova
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovska 6, CZ 77200 Olomouc, Czech Republic
    BMC Genomics 9:237. 2008
    ..6% of the total rye genome. The 1RS arm is an attractive target as it carries many important genes and because it became part of the wheat gene pool as the 1BL.1RS translocation...
  24. pmc A first survey of the rye (Secale cereale) genome composition through BAC end sequencing of the short arm of chromosome 1R
    Jan Bartos
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Olomouc, Czech Republic
    BMC Plant Biol 8:95. 2008
    ..1RS translocation. A better knowledge of the rye genome could facilitate rye improvement and increase the efficiency of utilizing rye genes in wheat breeding...
  25. pmc Flow sorting and sequencing meadow fescue chromosome 4F
    David Kopecky
    Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, 78371 Olomouc, Czech Republic
    Plant Physiol 163:1323-37. 2013
    ..Our results confirm that next-generation sequencing of flow-sorted chromosomes enables an overview of chromosome structure and evolution at a resolution never achieved before. ..
  26. ncbi request reprint Creation of a BAC resource to study the structure and evolution of the banana (Musa balbisiana) genome
    Jan Safar
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovska 6, CZ 77200 Olomouc, Czech Republic
    Genome 47:1182-91. 2004
    ..The PKW BAC library represents a publicly available tool, and is currently used to reveal the integration and activation mechanisms of BSV sequences and to study banana genome structure and evolution...
  27. ncbi request reprint Dissection of the nuclear genome of barley by chromosome flow sorting
    Pavla Suchánková
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovska 6, 77200 Olomouc, Czech Republic
    Theor Appl Genet 113:651-9. 2006
    ..This advance makes the flow cytogenetics an attractive tool, which may greatly facilitate genome analysis and gene cloning in barley...
  28. ncbi request reprint Flow cytometric chromosome sorting in plants: the next generation
    Jan Vrana
    Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Sokolovska 6, CZ 77200 Olomouc, Czech Republic
    Methods 57:331-7. 2012
    ..The methods outlined in this article describe a procedure for preparation of chromosomal DNA suitable for next-generation sequencing...
  29. pmc Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley
    Hana Simkova
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovska 6, CZ 77200 Olomouc, Czech Republic
    BMC Genomics 9:294. 2008
    ..Yet, many genomic applications such as development of genetic maps or physical mapping do not require large DNA fragments. In such cases time-consuming de novo sorting can be minimized by utilizing whole-genome amplification...
  30. doi request reprint Genomic constitution of Festuca × Lolium hybrids revealed by the DArTFest array
    David Kopecky
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovska 6, 77200, Olomouc, Czech Republic
    Theor Appl Genet 122:355-63. 2011
    ..While all bulks were suitable for identification of hybrids, only bulks of few plants have been found to reveal the rare markers...
  31. ncbi request reprint Dissecting large and complex genomes: flow sorting and BAC cloning of individual chromosomes from bread wheat
    Jan Safar
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovska 6, CZ 77200 Olomouc, Czech Republic
    Plant J 39:960-8. 2004
    ..This report on the successful cloning of flow-sorted chromosomes into BACs marks the integration of flow cytogenetics and genomics and represents a great leap forward in genetics and genomic analysis...
  32. ncbi request reprint Construction of a subgenomic BAC library specific for chromosomes 1D, 4D and 6D of hexaploid wheat
    Jaroslav Janda
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovska 6, 77200 Olomouc, Czech Republic
    Theor Appl Genet 109:1337-45. 2004
    ..Their availability should facilitate the analysis of wheat genome structure and evolution, development of cytogenetic maps, construction of local physical maps and map-based cloning of agronomically important genes...
  33. pmc BAC libraries from wheat chromosome 7D: efficient tool for positional cloning of aphid resistance genes
    Hana Simkova
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovska 6, CZ 772 00 Olomouc, Czech Republic
    J Biomed Biotechnol 2011:302543. 2011
    ..The small number of clones combined with high coverage made the screening highly efficient and cost effective...
  34. doi request reprint Integration of genetic and physical maps of the chickpea (Cicer arietinum L.) genome using flow-sorted chromosomes
    Pavlína Zatloukalová
    Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Sokolovska 6, 77200 Olomouc, Czech Republic
    Chromosome Res 19:729-39. 2011
    ..As a result, two clones were identified localizing specifically to chromosomes E and H, for which no cytogenetic markers were yet available...
  35. doi request reprint Genetic mapping of DArT markers in the Festuca-Lolium complex and their use in freezing tolerance association analysis
    Jan Bartos
    Institute of Experimental Botany, Sokolovska 6, 77200, Olomouc, Czech Republic
    Theor Appl Genet 122:1133-47. 2011
    ..The annotated DArTFest array resources could accelerate further studies and improvement of desired traits in Festuca-Lolium species...
  36. pmc Repetitive part of the banana (Musa acuminata) genome investigated by low-depth 454 sequencing
    Eva Hřibová
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovska 6, Olomouc CZ 77200, Czech Republic
    BMC Plant Biol 10:204. 2010
    ..This slows down the study of nuclear genome and the development of molecular tools to facilitate banana improvement...
  37. pmc Syntenic relationships between the U and M genomes of Aegilops, wheat and the model species Brachypodium and rice as revealed by COS markers
    Istvan Molnar
    Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvasar, Hungary
    PLoS ONE 8:e70844. 2013
    ....
  38. ncbi request reprint Flow cytometric chromosome sorting from diploid progenitors of bread wheat, T. urartu, Ae. speltoides and Ae. tauschii
    Istvan Molnar
    Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Brunszvik u 2, H 2462, Martonvasar, Hungary
    Theor Appl Genet 127:1091-104. 2014
    ..The isolation of individual chromosomes of wild progenitors provides an attractive opportunity to investigate the structure and evolution of the polyploid genome and to deliver tools for wheat improvement...
  39. pmc A multi gene sequence-based phylogeny of the Musaceae (banana) family
    Pavla Christelová
    Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Sokolovska 6, 772 00 Olomouc, Czech Republic
    BMC Evol Biol 11:103. 2011
    ..In this work the evolutionary relationships within the Musaceae family were studied using 13 species and DNA sequences obtained from a set of 19 unlinked nuclear genes...
  40. pmc Development and mapping of DArT markers within the Festuca - Lolium complex
    David Kopecky
    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovska 6, CZ 77200, Olomouc, Czech Republic
    BMC Genomics 10:473. 2009
    ..pratensis, F. arundinacea, F. glaucescens, L. perenne and L. multiflorum...
  41. pmc Intraspecific sequence comparisons reveal similar rates of non-collinear gene insertion in the B and D genomes of bread wheat
    Jan Bartos
    Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Sokolovska 6, Olomouc, CZ 77200, Czech Republic
    BMC Plant Biol 12:155. 2012
    ..As a result, the number of genes which are non-collinear to genomes of related species seems markedly increased in wheat...
  42. ncbi request reprint Duplicative transfer of a MADS box gene to a plant Y chromosome
    Sachihiro Matsunaga
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba, Japan
    Mol Biol Evol 20:1062-9. 2003
    ..Our results suggest that the acquisition of autosomal genes is an important event in the evolution of plant Y chromosomes...
  43. ncbi request reprint The origin, evolution and proposed stabilization of the terms 'genome size' and 'C-value' to describe nuclear DNA contents
    Johann Greilhuber
    Institute of Botany and Botanical Garden of the University of Vienna, Austria
    Ann Bot 95:255-60. 2005
    ..Reconsideration of the terminology is required...
  44. pmc Nuclear DNA content variation among Central European Koeleria taxa
    Ales Pecinka
    Institute for Plant Genetics and Crop Plant Research IPK, Corrensstrasse 3, Gatersleben, D 06466, Germany
    Ann Bot 98:117-22. 2006
    ..Therefore, these characteristics and their variability were determined for populations of six central European Koeleria taxa...
  45. pmc Flow cytometric and microscopic analysis of the effect of tannic acid on plant nuclei and estimation of DNA content
    João Loureiro
    Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitario de Santiago, 3810 193 Aveiro, Portugal
    Ann Bot 98:515-27. 2006
    ..In this study, the effect of tannic acid on estimation of nuclear DNA content was evaluated in Pisum sativum and Zea mays, which were chosen as model species...
  46. pmc Comparison of four nuclear isolation buffers for plant DNA flow cytometry
    João Loureiro
    Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitario de Santiago, 3810 193 Aveiro, Portugal
    Ann Bot 98:679-89. 2006
    ..This knowledge is required to select the appropriate buffer for a given species and tissue...
  47. doi request reprint Plant flow cytometry-far beyond the stone age
    João Loureiro
    Department of Botany, Faculty of Science, Charles University in Prague, Prague, Czech Republic
    Cytometry A 73:579-80. 2008
  48. pmc Two new nuclear isolation buffers for plant DNA flow cytometry: a test with 37 species
    João Loureiro
    Laboratory of Biotechnology and Cytomics, CESAM and Department of Biology, University of Aveiro, Campus Universitario de Santiago, 3810 193 Aveiro, Portugal
    Ann Bot 100:875-88. 2007
    ..In this work, recent data on the performance of nuclear isolation buffers are utilized in order to develop new buffers, general purpose buffer (GPB) and woody plant buffer (WPB), for plant DNA flow cytometry...
  49. ncbi request reprint MK17, a specific marker closely linked to the gynoecium suppression region on the Y chromosome in Silene latifolia
    Roman Hobza
    Laboratory of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska Street 135, 61265 Brno, Czech Republic
    Theor Appl Genet 113:280-7. 2006
    ..Hence MK17 represents a valuable marker to isolate genes controlling the gynoecium development suppression on the Y chromosome of S. latifolia...
  50. ncbi request reprint The bacterial artificial chromosome (BAC) library of the narrow-leafed lupin (Lupinus angustifolius L.)
    Andrzej Kasprzak
    Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, Poznan, Poland
    Cell Mol Biol Lett 11:396-407. 2006
    ..The availability of a BAC library opens avenues for the development of a physical contig map and positional gene cloning, as well as for the analysis of the plant's genome structure and evolution...
  51. ncbi request reprint PRINS on plant chromosomes
    Jiri Macas
    Institute of Plant Molecular Biology, Ceske Budejovice, Czech Republic
    Methods Mol Biol 334:133-9. 2006
    ..This approach allows the distinguishing of sequences that differ in only a few nucleotides and has proved to be valuable for studies of satellite DNA evolution in plants...