Jaroslav Dolezel

..These experiments enabled assignment of the linkage groups IV and VII to chromosomes 4 and 7, respectively...

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..This physical map establishes a template for the remaining wheat chromosomes and demonstrates the feasibility of constructing physical maps in large, complex, polyploid genomes with a chromosome-based approach...

..This paper provides a comprehensive review of the methodology and application of flow cytogenetics, and assesses its potential for plant genome analysis...

..This review explains the principles of flow cytometric chromosome analysis and sorting (flow cytogenetics), discusses the major uses of this technology in genome analysis, and outlines future directions...

..abstract:..

..Recently, the gene AP3 was characterized in the dioecious plant species Silene latifolia. It was suggested that this gene had been transferred from an autosome to the Y chromosome...

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..Chromosomes isolated according to this protocol have well-preserved morphology, withstand shearing forces during sorting, and their DNA is intact and suitable for a range of applications...

..While the former applications are relatively straightforward, estimation of absolute DNA amount requires special attention to possible errors in sample preparation and analysis...

..The purpose and requirement of internal and external standardization are discussed. The importance of using a correct terminology for DNA amounts and genome size is underlined, and its basic principles are explained...

..This article assesses the current state of DNA reference standards for flow cytometry and the issues related to their calibration...

..This study considerably expands the potential of flow cytogenetics for use in wheat genomics and opens the possibility of sequencing the genome of this important crop one chromosome arm at a time...

..6% of the total rye genome. The 1RS arm is an attractive target as it carries many important genes and because it became part of the wheat gene pool as the 1BL.1RS translocation...

..1RS translocation. A better knowledge of the rye genome could facilitate rye improvement and increase the efficiency of utilizing rye genes in wheat breeding...

..The PKW BAC library represents a publicly available tool, and is currently used to reveal the integration and activation mechanisms of BSV sequences and to study banana genome structure and evolution...

..This advance makes the flow cytogenetics an attractive tool, which may greatly facilitate genome analysis and gene cloning in barley...

..The methods outlined in this article describe a procedure for preparation of chromosomal DNA suitable for next-generation sequencing...

..While all bulks were suitable for identification of hybrids, only bulks of few plants have been found to reveal the rare markers...

..Yet, many genomic applications such as development of genetic maps or physical mapping do not require large DNA fragments. In such cases time-consuming de novo sorting can be minimized by utilizing whole-genome amplification...

..As a result, two clones were identified localizing specifically to chromosomes E and H, for which no cytogenetic markers were yet available...

..The small number of clones combined with high coverage made the screening highly efficient and cost effective...

..The annotated DArTFest array resources could accelerate further studies and improvement of desired traits in Festuca-Lolium species...

..pratensis, F. arundinacea, F. glaucescens, L. perenne and L. multiflorum...

..Their availability should facilitate the analysis of wheat genome structure and evolution, development of cytogenetic maps, construction of local physical maps and map-based cloning of agronomically important genes...

..This slows down the study of nuclear genome and the development of molecular tools to facilitate banana improvement...

..In this work the evolutionary relationships within the Musaceae family were studied using 13 species and DNA sequences obtained from a set of 19 unlinked nuclear genes...

..With the aim of providing a simple and robust approach for molecular characterization of Musa species, we developed an optimized genotyping platform using 19 published simple sequence repeat markers...

..This report on the successful cloning of flow-sorted chromosomes into BACs marks the integration of flow cytogenetics and genomics and represents a great leap forward in genetics and genomic analysis...

..As a result, the number of genes which are non-collinear to genomes of related species seems markedly increased in wheat...

..The observation of only one type of ITS sequence in some of the presumed interspecific hybrid clones warrants further study to confirm their hybrid origin and to unravel processes leading to evolution of their genomes...

..Our results suggest that the acquisition of autosomal genes is an important event in the evolution of plant Y chromosomes...

..g. haploids, hybrids, etc.) which will need minor extensions of the present scheme in a future paper...

..Therefore, these characteristics and their variability were determined for populations of six central European Koeleria taxa...

..In this study, the effect of tannic acid on estimation of nuclear DNA content was evaluated in Pisum sativum and Zea mays, which were chosen as model species...

..This knowledge is required to select the appropriate buffer for a given species and tissue...

..In this work, recent data on the performance of nuclear isolation buffers are utilized in order to develop new buffers, general purpose buffer (GPB) and woody plant buffer (WPB), for plant DNA flow cytometry...

..Hence MK17 represents a valuable marker to isolate genes controlling the gynoecium development suppression on the Y chromosome of S. latifolia...

..The availability of a BAC library opens avenues for the development of a physical contig map and positional gene cloning, as well as for the analysis of the plant's genome structure and evolution...

..This approach allows the distinguishing of sequences that differ in only a few nucleotides and has proved to be valuable for studies of satellite DNA evolution in plants...