Jiri Drabek

Summary

Affiliation: Palacky University
Location: Olomouc, Czech Republic
URL: www.dnabased.com
Summary:
Optimization and validation of molecular genetics methods
Paternity and relationship testing
Quality management
Supervising of laboratory technicians
Supervising of university theses
Reviewing grant applications and manuscripts
Expert reporting

Publications

  1. ncbi Familial hydronephrosis unlinked to the HLA complex
    A Santava
    Department of Medical Genetics and Fetal Medicine, Palacky University, Olomouc, Czech Republic
    Am J Med Genet 70:118-20. 1997
  2. ncbi A sugar, laundry detergent, and salt method for extraction of deoxyribonucleic acid from blood
    Jiri Drabek
    Department of Immunology, Palacky University and University Hospital, Olomouc, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 146:37-9. 2002
  3. ncbi Immunohistochemical study of the apoptotic mechanisms in the intestinal mucosa during children's coeliac disease
    Jiri Ehrmann
    Laboratory of Molecular Pathology and Institute of Pathology, Palacky University, Hnevotínská 3 77515, Olomouc, Czech Republic
    Virchows Arch 442:453-61. 2003
  4. ncbi CC chemokine receptor (CCR)2 polymorphism in Czech patients with myocardial infarction
    Jana Petrkova
    Department of Immunology, Palacky University, I P Pavlova str 6, Olomouc, Czech Republic
    Immunol Lett 88:53-5. 2003
  5. ncbi A study on the effects of degradation and template concentration on the amplification efficiency of the STR Miniplex primer sets
    Denise T Chung
    Ohio University, Department of Chemistry and Biochemistry, Athens, OH 45701, USA
    J Forensic Sci 49:733-40. 2004
  6. ncbi Concordance study between Miniplex assays and a commercial STR typing kit
    Jiri Drabek
    Ohio University, Department of Chemistry and Biochemistry, Athens, OH 45701, USA
    J Forensic Sci 49:859-60. 2004
  7. ncbi The MCP-1 -2518 (A to G) single nucleotide polymorphism is not associated with myocardial infarction in the Czech population
    Z Cermakova
    Immunology and Immunogenetics, Palacky University and Faculty Hospital Olomouc, Czech Republic
    Int J Immunogenet 32:315-8. 2005
  8. ncbi The application of miniplex primer sets in the analysis of degraded DNA from human skeletal remains
    Kerry L Opel
    Department of Chemistry and Biochemistry, Florida International University, University Park, 11200 SW, FL 33199, USA
    J Forensic Sci 51:351-6. 2006
  9. ncbi A duplication dup(4)(q28q35.2) de novo in a newborn
    Iveta Cernakova
    Institute of Biology and Medical Genetics, General Faculty Hospital and The 1st Faculty of Medicine, Charles University, Prague 2, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 150:113-6. 2006
  10. ncbi Complement receptor 1 single nucleotide polymorphisms in Czech and Dutch patients with sarcoidosis
    F Mrazek
    Immunology and Respiratory Medicine, Palacky University, Olomouc, Czech Republic
    Tissue Antigens 71:77-80. 2008

Collaborators

Detail Information

Publications29

  1. ncbi Familial hydronephrosis unlinked to the HLA complex
    A Santava
    Department of Medical Genetics and Fetal Medicine, Palacky University, Olomouc, Czech Republic
    Am J Med Genet 70:118-20. 1997
    ..Although formal linkage calculations are not presented, it is apparent that in some families HLA haplotyping is not useful in predicting prevence of renal obstruction...
  2. ncbi A sugar, laundry detergent, and salt method for extraction of deoxyribonucleic acid from blood
    Jiri Drabek
    Department of Immunology, Palacky University and University Hospital, Olomouc, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 146:37-9. 2002
    ..Our method is simple, fast, and inexpensive; its qualitative parameters do not significantly differ from the standard salting-out procedure...
  3. ncbi Immunohistochemical study of the apoptotic mechanisms in the intestinal mucosa during children's coeliac disease
    Jiri Ehrmann
    Laboratory of Molecular Pathology and Institute of Pathology, Palacky University, Hnevotínská 3 77515, Olomouc, Czech Republic
    Virchows Arch 442:453-61. 2003
    ..Our results showed that Fas and/or Fas-L, Bcl-2, and tTG may be involved in apoptotic pathways leading to mucosal atrophy in children with CD. tTG changes are in agreement with the presumed role of this protein in the pathogenesis of CD...
  4. ncbi CC chemokine receptor (CCR)2 polymorphism in Czech patients with myocardial infarction
    Jana Petrkova
    Department of Immunology, Palacky University, I P Pavlova str 6, Olomouc, Czech Republic
    Immunol Lett 88:53-5. 2003
    ..01). If the VI genotype of the CCR2-V64I is indeed a risk factor for an earlier MI onset in females must be checked by independent studies in other centres and/or populations...
  5. ncbi A study on the effects of degradation and template concentration on the amplification efficiency of the STR Miniplex primer sets
    Denise T Chung
    Ohio University, Department of Chemistry and Biochemistry, Athens, OH 45701, USA
    J Forensic Sci 49:733-40. 2004
    ..Overall, our data confirm that our redesigned primers can increase the probability of obtaining a usable profile in situations where standard kits fail...
  6. ncbi Concordance study between Miniplex assays and a commercial STR typing kit
    Jiri Drabek
    Ohio University, Department of Chemistry and Biochemistry, Athens, OH 45701, USA
    J Forensic Sci 49:859-60. 2004
  7. ncbi The MCP-1 -2518 (A to G) single nucleotide polymorphism is not associated with myocardial infarction in the Czech population
    Z Cermakova
    Immunology and Immunogenetics, Palacky University and Faculty Hospital Olomouc, Czech Republic
    Int J Immunogenet 32:315-8. 2005
    ..The data do not support a role for the MCP-1 -2518 single nucleotide polymorphism in susceptibility to CAD manifested by myocardial infarction...
  8. ncbi The application of miniplex primer sets in the analysis of degraded DNA from human skeletal remains
    Kerry L Opel
    Department of Chemistry and Biochemistry, Florida International University, University Park, 11200 SW, FL 33199, USA
    J Forensic Sci 51:351-6. 2006
    ..These data suggest smaller PCR amplicons may provide a useful alternative to mitochondrial DNA for anthropological and forensic analysis of degraded DNA from human skeletal remains...
  9. ncbi A duplication dup(4)(q28q35.2) de novo in a newborn
    Iveta Cernakova
    Institute of Biology and Medical Genetics, General Faculty Hospital and The 1st Faculty of Medicine, Charles University, Prague 2, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 150:113-6. 2006
    ..We compare in this report similarity of the clinical picture of our patient, NBS cases and other patients carrying a duplication of the distal part of 4q as described in the literature...
  10. ncbi Complement receptor 1 single nucleotide polymorphisms in Czech and Dutch patients with sarcoidosis
    F Mrazek
    Immunology and Respiratory Medicine, Palacky University, Olomouc, Czech Republic
    Tissue Antigens 71:77-80. 2008
    ....
  11. ncbi Developmental validation of reduced-size STR Miniplex primer sets
    Kerry L Opel
    International Forensic Research Institute, Florida International University, 11200 SW 8th Street, Miami, FL 33199, USA
    J Forensic Sci 52:1263-71. 2007
    ..Our results demonstrate that Miniplex STR amplification procedures are a robust and sensitive tool for the analysis of degraded DNA...
  12. ncbi Validation of software for calculating the likelihood ratio for parentage and kinship
    J Drabek
    LEM, Faculty of Medicine, Palacky University, Puskinova 6, 77520 Olomouc, Czech Republic
    Forensic Sci Int Genet 3:112-8. 2009
    ....
  13. ncbi Clinical relevance of KRAS in human cancers
    Sylwia Jancík
    Laboratory of Experimental Medicine, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University and University Hospital, 775 20 Olomouc, Czech Republic
    J Biomed Biotechnol 2010:150960. 2010
    ....
  14. ncbi Frequency of 657del(5) mutation of the NBS1 gene in the Czech population by polymerase chain reaction with sequence specific primers
    Jiri Drabek
    Department of Immunology, Faculty of Medicine, Palacky University and Faculty Hospital in Olomouc, Olomouc, Czech Republic
    Cancer Genet Cytogenet 138:157-9. 2002
    ....
  15. ncbi CC chemokine receptor 5 and interleukin-1 receptor antagonist gene polymorphisms in patients with primary Sjögren's syndrome
    M Petrek
    Department of Immunology, Tissue Typing Laboratory, Medical Faculty of Palacky University, I P Pavlova no 6, 775 20 Olomouc, Czech Republic
    Clin Exp Rheumatol 20:701-3. 2002
    ..We have therefore in a case control study assessed the putative role of these "candidate" polymorphic genes in the inflammatory process in Sjögren syndrome...
  16. ncbi [Methods of expressing the strength of expert proof of paternity determination]
    J Drabek
    Ustav imunologie FN a LF UP, Olomouc
    Cas Lek Cesk 141:371-5. 2002
    ..Advantage of likelihood ratio is described and demonstrated on microsatellite typing examples...
  17. ncbi 32 bp deletion in CCR-5 gene and human immunodeficiency virus epidemic in the Czech Republic
    J Drabek
    Acta Virol 42:121-2. 1998
  18. ncbi Class I typing by PCR-SSP in Olomouc
    J Drabek
    Department of Immunology, Teaching Hospital, Olomouc, The Czech Republic
    Bone Marrow Transplant 22:S23-6. 1998
    ..Our experience and tips are described below. Population data of healthy unrelated individuals for HLA-A, -B, -Cw are given in the tables...
  19. ncbi Characterization of a novel HLA-A*24 allele containing an HLA-A*03 sequence motif
    J Drabek
    Department of Immunology, Teaching Hospital, Olomouc, The Czech Republic
    Tissue Antigens 54:98-101. 1999
    ..Results from nucleotide sequencing analyses of polymerase chain reaction (PCR) amplification products derived from genomic and cDNA are compatible with those findings...
  20. ncbi Diversity among wild type and vaccination strains of Trichophyton verrucosum investigated using random amplified polymorphic DNA analysis
    M Hajduch
    Department of Paediatrics, Faculty of Medicine, Palacky University and Faculty Hospital in Olomouc, Czech Republic
    Folia Biol (Praha) 45:151-6. 1999
    ..No marker for a/virulence was detected. These outcomes suggest new possibilities for epidemiological analyses, for discrimination among different vaccination strains and studies of fungal population in vaccinated/infected hosts...
  21. ncbi CC chemokine receptor gene polymorphisms in Czech patients with pulmonary sarcoidosis
    M Petrek
    Departments of Immunology and Respiratory Medicine, PalackýUniversity, Olomouc, Czech Republic
    Am J Respir Crit Care Med 162:1000-3. 2000
    ..01). In conclusion, the observed association of CCR5Delta32 and CCR2-64I with sarcoidosis implicates a role for these polymorphisms in disease susceptibility and protection...
  22. ncbi A commented dictionary of techniques for genotyping
    J Drabek
    Department of Immunology, Olomouc, Czech Republic
    Electrophoresis 22:1024-45. 2001
    ..The distinguishing principle for each method is briefly explained. Sorting of and discussion on the methods give the reader a brief introduction to the field of genotyping...
  23. ncbi Non-expression of HLA-B*5111N is caused by an insertion into the cytosine island at exon 4 creating a frameshift stop codon
    H A Elsner
    Department of Transfusion Medicine, Hannover Medical School, Hannover, Germany
    Tissue Antigens 57:369-72. 2001
    ..In order not to overlook the presence of alleles with altered expression in case of hematopoietic stem cell transplantation, both serological and DNA-based typing should be performed (Note)...
  24. ncbi Human leukocyte antigen-DRB1 position 11 residues are a common protective marker for sarcoidosis
    P J Foley
    Interstitial Lung Disease Unit, Department of Occupational and Environmental Medicine, National Heart and Lung Institute, Imperial College of Science, Technology, and Medicine, London, UK
    Am J Respir Cell Mol Biol 25:272-7. 2001
    ..This suggests this HLA-DR residue is an important protective marker in sarcoidosis...
  25. ncbi [Occurrence of celiac disease in siblings and offspring of patients with celiac disease]
    A Kolek
    Dĕtská klinika FN a LF UP, Olomouc
    Cas Lek Cesk 140:695-8. 2001
    ..3 to 0.5%, however, in direct relatives of patients with coeliac disease incidence rises up to 5-15%. Though the disease in not always clinically manifested, it can be identified by serologic screening...
  26. ncbi Interleukin-1 gene cluster polymorphisms in sarcoidosis and idiopathic pulmonary fibrosis
    Beata Hutyrova
    Departments of Immunology, Biology, and Respiratory Medicine, Palacky University, Olomouc, Czech Republic
    Am J Respir Crit Care Med 165:148-51. 2002
    ..Further population studies are warranted to confirm the observed association between sarcoidosis and the IL-1alpha polymorphism and also to explore mechanisms of IL-1alpha -889 participation in aberrant immune response in sarcoidosis...
  27. ncbi CC chemokine receptor 5 (CCR5) mRNA expression in pulmonary sarcoidosis
    Martin Petrek
    Department of Immunology, Palacky University, I P Pavlova Street 6, Olomouc CZ 775 20, Czech Republic
    Immunol Lett 80:189-93. 2002
    ..Though the data from this pilot study had no clinical correlations we suggest that further studies are warranted on the role of this Th1 subset marker in the pathogenesis of sarcoidosis...
  28. ncbi Expression of the chemokine PARC mRNA in bronchoalveolar cells of patients with sarcoidosis
    Frantisek Mrazek
    Department of Immunology, Palacky University, I P Pavlova str 6, CZ 775 20, Olomouc, Czech Republic
    Immunol Lett 84:17-22. 2002
    ..Further, because several immunomodulators are used in the treatment of sarcoidosis, we have determined the effects of selected drugs on PARC mRNA expression in vitro...
  29. ncbi [Principles of dealing with human DNA and genotyping information]
    Jiri Drabek
    Ustav Molekulární a Translační, Lékarská fakulta Univerzity Palackého, Olomouc Československá Společnost pro Forenzní Genetiku, Olomouc
    Cas Lek Cesk 151:507-13. 2012
    ..We believe that it is a worthwhile task...