M Stiborova

Summary

Affiliation: Charles University
Country: Czech Republic

Publications

  1. doi request reprint Cytochrome b5 and epoxide hydrolase contribute to benzo[a]pyrene-DNA adduct formation catalyzed by cytochrome P450 1A1 under low NADPH:P450 oxidoreductase conditions
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic Electronic address
    Toxicology 318:1-12. 2014
  2. ncbi request reprint The effect of benzo[a]pyrene on metabolic activation of anticancer drug ellipticine in mice
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 34:43-54. 2013
  3. ncbi request reprint Oxidation of carcinogenic benzo[a]pyrene by human and rat cytochrome P450 1A1 and its influencing by cytochrome b5 - a comparative study
    Radek Indra
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 34:55-63. 2013
  4. ncbi request reprint Ellipticines as DNA-targeted chemotherapeutics
    Marie Stiborova
    Department of Biochemistry, Charles University in Prague, Czech Republic
    Curr Med Chem 21:575-91. 2014
  5. ncbi request reprint Enzymes metabolizing aristolochic acid and their contribution to the development of aristolochic acid nephropathy and urothelial cancer
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University Prague, Albertov 2030, 128 40 Prague 2, Czech Republic
    Curr Drug Metab 14:695-705. 2013
  6. doi request reprint Induced expression of cytochrome P450 1A and NAD(P)H:quinone oxidoreductase determined at mRNA, protein, and enzyme activity levels in rats exposed to the carcinogenic azo dye 1-phenylazo-2-naphthol (Sudan I)
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic
    Chem Res Toxicol 26:290-9. 2013
  7. ncbi request reprint Effects of cytochrome P450 inhibitors on peroxidase activity
    Marketa Martinkova
    Department of Biochemistry, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 33:33-40. 2012
  8. ncbi request reprint Theoretical investigation of differences in nitroreduction of aristolochic acid I by cytochromes P450 1A1, 1A2 and 1B1
    Petr Jerabek
    Department of Biochemistry, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 33:25-32. 2012
  9. ncbi request reprint Activation and detoxification metabolism of urban air pollutants 2-nitrobenzanthrone and carcinogenic 3-nitrobenzanthrone by rat and mouse hepatic microsomes
    Marie Stiborova
    Department of Biochemistry, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 33:8-15. 2012
  10. ncbi request reprint Impact of histone deacetylase inhibitor valproic acid on the anticancer effect of etoposide on neuroblastoma cells
    Tomas Groh
    Department of Biochemistry, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 33:16-24. 2012

Collaborators

Detail Information

Publications86

  1. doi request reprint Cytochrome b5 and epoxide hydrolase contribute to benzo[a]pyrene-DNA adduct formation catalyzed by cytochrome P450 1A1 under low NADPH:P450 oxidoreductase conditions
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic Electronic address
    Toxicology 318:1-12. 2014
    ..Our results suggest that in livers of HRN mice Cyp1a1, cytochrome b5 and mEH can effectively activate BaP to DNA binding species, even in the presence of very low amounts of POR. ..
  2. ncbi request reprint The effect of benzo[a]pyrene on metabolic activation of anticancer drug ellipticine in mice
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 34:43-54. 2013
    ....
  3. ncbi request reprint Oxidation of carcinogenic benzo[a]pyrene by human and rat cytochrome P450 1A1 and its influencing by cytochrome b5 - a comparative study
    Radek Indra
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 34:55-63. 2013
    ..The aim of this work was to compare BaP oxidation by human CYP1A1 and CYP1A1 of one animal model, rat. Investigation of the effect of cytochrome b5 on BaP oxidation by CYP1A1 was another target of this study...
  4. ncbi request reprint Ellipticines as DNA-targeted chemotherapeutics
    Marie Stiborova
    Department of Biochemistry, Charles University in Prague, Czech Republic
    Curr Med Chem 21:575-91. 2014
    ..The aim of this review is to summarize our knowledge on the molecular mechanisms with the aim to explain the effectiveness of ellipticines as DNA-targeted chemotherapeutics in cancer cells. ..
  5. ncbi request reprint Enzymes metabolizing aristolochic acid and their contribution to the development of aristolochic acid nephropathy and urothelial cancer
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University Prague, Albertov 2030, 128 40 Prague 2, Czech Republic
    Curr Drug Metab 14:695-705. 2013
    ..Molecular modeling also shows why CYP1A2 plays an important role in the oxidation of AAI to AAIa. ..
  6. doi request reprint Induced expression of cytochrome P450 1A and NAD(P)H:quinone oxidoreductase determined at mRNA, protein, and enzyme activity levels in rats exposed to the carcinogenic azo dye 1-phenylazo-2-naphthol (Sudan I)
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic
    Chem Res Toxicol 26:290-9. 2013
    ..In addition, the induction of P450 1A1/2 and NQO1 enzymes can influence individual human susceptibility to other environmental carcinogens and have an effect on cancer risk...
  7. ncbi request reprint Effects of cytochrome P450 inhibitors on peroxidase activity
    Marketa Martinkova
    Department of Biochemistry, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 33:33-40. 2012
    ..The aim of this study was to investigate specificities of several known CYP inhibitors to these enzymes; whether they inhibit only the CYP enzymes and do not inhibit peroxidases...
  8. ncbi request reprint Theoretical investigation of differences in nitroreduction of aristolochic acid I by cytochromes P450 1A1, 1A2 and 1B1
    Petr Jerabek
    Department of Biochemistry, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 33:25-32. 2012
    ..However, knowledge of the differences in mechanistic details of CYP1A1-, 1A2-, and 1B1- mediated reduction is still lacking. Therefore, this feature is the aim of the present study...
  9. ncbi request reprint Activation and detoxification metabolism of urban air pollutants 2-nitrobenzanthrone and carcinogenic 3-nitrobenzanthrone by rat and mouse hepatic microsomes
    Marie Stiborova
    Department of Biochemistry, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 33:8-15. 2012
    ..In addition, using the same microsomal systems, 2-NBA and 3-NBA were evaluated to be enzymatically activated under anaerobic conditions to species generating 2-NBA- and 3-NBA-derived DNA adducts...
  10. ncbi request reprint Impact of histone deacetylase inhibitor valproic acid on the anticancer effect of etoposide on neuroblastoma cells
    Tomas Groh
    Department of Biochemistry, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 33:16-24. 2012
    ..In this study, we investigated the effect of this anticancer drug alone and in combination with a histone deacetylase (HDAC) inhibitor, valproic acid (VPA), on a human UKF-NB-4 neuroblastoma cell line...
  11. doi request reprint Ellipticine oxidation and DNA adduct formation in human hepatocytes is catalyzed by human cytochromes P450 and enhanced by cytochrome b5
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic
    Toxicology 302:233-41. 2012
    ..7 μM. In liver CYP3A4 is the predominant ellipticine activating CYP species, which is expected to result in efficient metabolism after oral ingestion of ellipticine in humans...
  12. ncbi request reprint The synergistic effects of DNA-targeted chemotherapeutics and histone deacetylase inhibitors as therapeutic strategies for cancer treatment
    M Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Curr Med Chem 19:4218-38. 2012
    ..Several molecular mechanisms are responsible for the observed higher sensitivity of tumor cells towards therapeutic agents elicited by HDAC inhibitors. These mechanisms are discussed also in this review...
  13. doi request reprint Cytochrome b5 increases cytochrome P450 3A4-mediated activation of anticancer drug ellipticine to 13-hydroxyellipticine whose covalent binding to DNA is elevated by sulfotransferases and N,O-acetyltransferases
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, Prague 2, Czech Republic
    Chem Res Toxicol 25:1075-85. 2012
    ..The calculated reaction free energies of heterolysis of the sulfate and acetate esters are by 10-17 kcal/mol more favorable than the energy of hydrolysis of 13-hydroxyellipticine, which could explain the experimental data...
  14. ncbi request reprint Role of cytochromes P450 in metabolism of carcinogenic aristolochic acid I: evidence of their contribution to aristolochic acid I detoxication and activation in rat liver
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 32:121-30. 2011
    ..Therefore, the present study has been designed to evaluate the cytochrome P450 (CYP)-mediated oxidative detoxification and reductive activation of AAI in a rat model...
  15. pmc Bioactivation versus detoxication of the urothelial carcinogen aristolochic acid I by human cytochrome P450 1A1 and 1A2
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, 12840 Prague 2, Czech Republic
    Toxicol Sci 125:345-58. 2012
    ....
  16. ncbi request reprint Metabolic activation of carcinogenic aristolochic acid, a risk factor for Balkan endemic nephropathy
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic
    Mutat Res 658:55-67. 2008
    ....
  17. ncbi request reprint Formation and persistence of DNA adducts of anticancer drug ellipticine in rats
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 12840 Prague 2, Czech Republic
    Toxicology 236:50-60. 2007
    ..These results, the first characterization of persistence of ellipticine-DNA adducts in vivo, are necessary to evaluate genotoxic side effects of ellipticine...
  18. ncbi request reprint Oxidation pattern of the anticancer drug ellipticine by hepatic microsomes - similarity between human and rat systems
    M Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic
    Gen Physiol Biophys 25:245-61. 2006
    ..The results underline the suitability of rat species as a model to evaluate human susceptibility to ellipticine...
  19. ncbi request reprint Modulation of CYP1A1-mediated oxidation of carcinogenic azo dye Sudan I and its binding to DNA by cytochrome b5
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 27:35-9. 2006
    ..Another aim of the study was to examine the formation of Sudan I-DNA adducts in vivo...
  20. ncbi request reprint Mammalian peroxidases activate anticancer drug ellipticine to intermediates forming deoxyguanosine adducts in DNA identical to those found in vivo and generated from 12-hydroxyellipticine and 13-hydroxyellipticine
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Int J Cancer 120:243-51. 2007
    ..The study forms the basis to further predict the susceptibility of human cancers to ellipticine...
  21. ncbi request reprint Molecular mechanisms of antineoplastic action of an anticancer drug ellipticine
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, Prague 2, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 150:13-23. 2006
    ..The study forms the basis to further predict the susceptibility of human cancers to ellipticine...
  22. ncbi request reprint Role of hepatic cytochromes P450 in bioactivation of the anticancer drug ellipticine: studies with the hepatic NADPH:cytochrome P450 reductase null mouse
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic
    Toxicol Appl Pharmacol 226:318-27. 2008
    ....
  23. doi request reprint The environmental pollutant and carcinogen 3-nitrobenzanthrone induces cytochrome P450 1A1 and NAD(P)H:quinone oxidoreductase in rat lung and kidney, thereby enhancing its own genotoxicity
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic
    Toxicology 247:11-22. 2008
    ..These results demonstrate that 3-NBA is capable to induce NQO1 and CYP1A1 in lungs and kidney of rats thereby enhancing its own genotoxic and carcinogenic potential...
  24. doi request reprint Biotransformation enzymes in development of renal injury and urothelial cancer caused by aristolochic acid
    M Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Kidney Int 73:1209-11. 2008
    ..Xiao et al. demonstrate that hepatic cytochrome P450s in mice detoxicate AA and thereby protect kidney from injury. The relative contribution of enzymes activating AA to induce urothelial cancer in humans remains to be resolved...
  25. doi request reprint 3-aminobenzanthrone, a human metabolite of the carcinogenic environmental pollutant 3-nitrobenzanthrone, induces biotransformation enzymes in rat kidney and lung
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic
    Mutat Res 676:93-101. 2009
    ....
  26. doi request reprint Mechanisms of the different DNA adduct forming potentials of the urban air pollutants 2-nitrobenzanthrone and carcinogenic 3-nitrobenzanthrone
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, Prague 2, Czech Republic
    Chem Res Toxicol 23:1192-201. 2010
    ..These findings explain the very different genotoxicity, mutagenicity, and DNA adduct forming potential of the two compounds. Collectively, our results suggest that 2-NBA possesses a relatively lower risk to humans than 3-NBA...
  27. doi request reprint Cytochrome P450- and peroxidase-mediated oxidation of anticancer alkaloid ellipticine dictates its anti-tumor efficiency
    M Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 12840 Prague 2, Czech Republic
    Biochim Biophys Acta 1814:175-85. 2011
    ..It also suggests ellipticine reactive metabolites 13-hydroxyellipticine and 12-hydroxyellipticine to be good candidates for targeting to tumors absent from the CYP and peroxidase activation enzymes...
  28. ncbi request reprint Role of cytochromes P450 and peroxidases in metabolism of the anticancer drug ellipticine: additional evidence of their contribution to ellipticine activation in rat liver, lung and kidney
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 31:26-35. 2010
    ..The contribution of pulmonary and renal CYP- and peroxidase enzymes to ellipticine metabolic activation is investigated and compared with that found in the liver...
  29. doi request reprint The human carcinogen aristolochic acid i is activated to form DNA adducts by human NAD(P)H:quinone oxidoreductase without the contribution of acetyltransferases or sulfotransferases
    Marie Stiborova
    Department of Biochemistry, Charles University, Albertov, Prague, Czech Republic
    Environ Mol Mutagen 52:448-59. 2011
    ..These results emphasize the major importance of NQO1 in the metabolic activation of AAI and provide the first evidence that initial nitroreduction is the rate limiting step in AAI activation...
  30. ncbi request reprint The environmental pollutant and carcinogen 3-nitrobenzanthrone and its human metabolite 3-aminobenzanthrone are potent inducers of rat hepatic cytochromes P450 1A1 and -1A2 and NAD(P)H:quinone oxidoreductase
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic
    Drug Metab Dispos 34:1398-405. 2006
    ....
  31. ncbi request reprint DNA adduct formation by the anticancer drug ellipticine in rats determined by 32P postlabeling
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030 40 Prague 2, Czech Republic
    Int J Cancer 107:885-90. 2003
    ..The results presented here are the first report showing the formation of CYP-mediated covalent DNA adducts by ellipticine in vivo and confirm the formation of covalent DNA adducts as a new mode of ellipticine action...
  32. ncbi request reprint The anticancer drug ellipticine forms covalent DNA adducts, mediated by human cytochromes P450, through metabolism to 13-hydroxyellipticine and ellipticine N2-oxide
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Cancer Res 64:8374-80. 2004
    ..Homologue modeling and docking of ellipticine to the CYP3A4 active center was used to explain the predominance of ellipticine oxidation by CYP3A4 to 13-hydroxyellipticine and N(2)-oxide...
  33. ncbi request reprint Human hepatic and renal microsomes, cytochromes P450 1A1/2, NADPH:cytochrome P450 reductase and prostaglandin H synthase mediate the formation of aristolochic acid-DNA adducts found in patients with urothelial cancer
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic
    Int J Cancer 113:189-97. 2005
    ..In addition, prostaglandin H synthase is another enzyme activating AAI in renal microsomes. The results demonstrate for the first time the potential of microsomal enzymes in human liver and kidney to activate AAI by nitroreduction...
  34. ncbi request reprint Carcinogenic and nephrotoxic alkaloids aristolochic acids upon activation by NADPH : cytochrome P450 reductase form adducts found in DNA of patients with Chinese herbs nephropathy
    M Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Gen Physiol Biophys 20:375-92. 2001
    ..The results presented here are the first report demonstrating a reductive activation of natural nitroaromatic compounds, AA, by NADPH: CYP reductase...
  35. ncbi request reprint DNA adduct formation from quaternary benzo[c]phenanthridine alkaloids sanguinarine and chelerythrine as revealed by the 32P-postlabeling technique
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 2 Prague, Czech Republic
    Chem Biol Interact 140:231-42. 2002
    ..Our results demonstrate that sanguinarine and chelerythrine are metabolized by hepatic microsomes to species, which generate DNA adducts...
  36. ncbi request reprint Hydroxylation of phenol to catechol by Candida tropicalis: involvement of cytochrome P450
    M Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Gen Physiol Biophys 22:167-79. 2003
    ..The data demonstrate the progress in resolving the enzymes responsible for the first step of phenol degradation by the C. tropicalis strain...
  37. ncbi request reprint Sudan I is a potential carcinogen for humans: evidence for its metabolic activation and detoxication by human recombinant cytochrome P450 1A1 and liver microsomes
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, 128 40 Prague 2, The Czech Republic
    Cancer Res 62:5678-84. 2002
    ..These results, the first report on the metabolism of Sudan I by human CYP enzymes, strongly suggest a carcinogenic potency of this rodent carcinogen for humans...
  38. ncbi request reprint Rat microsomes activating the anticancer drug ellipticine to species covalently binding to deoxyguanosine in DNA are a suitable model mimicking ellipticine bioactivation in humans
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 12840 Prague 2, Czech Republic
    Chem Res Toxicol 16:38-47. 2003
    ..The results strongly suggest that rats are more suitable models than rabbits mimicking the metabolic activation of ellipticine in humans...
  39. ncbi request reprint Identification of a genotoxic mechanism for 2-nitroanisole carcinogenicity and of its carcinogenic potential for humans
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic
    Carcinogenesis 25:833-40. 2004
    ..The results of our study, the first report on the potential of human cytosolic enzymes to contribute to the activation of 2-NA by nitroreduction, strongly suggest a carcinogenic potency of this rodent carcinogen for humans...
  40. ncbi request reprint Human cytosolic enzymes involved in the metabolic activation of carcinogenic aristolochic acid: evidence for reductive activation by human NAD(P)H:quinone oxidoreductase
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, The Czech Republic
    Carcinogenesis 24:1695-703. 2003
    ..The orientation of AAI in the active site of human NQO1 was predicted from molecular modeling based on published X-ray structures. The results demonstrate for the first time the potential of human NQO1 to activate AAI by nitroreduction...
  41. ncbi request reprint Expression of cytochrome P450 1A1 and its contribution to oxidation of a potential human carcinogen 1-phenylazo-2-naphthol (Sudan I) in human livers
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic
    Cancer Lett 220:145-54. 2005
    ..Even though levels of CYP1A1 expression are low, <0.7% of total hepatic CYP, the CYP1A1 contribution to oxidation of carcinogenic Sudan I in the test set of human liver microsomes ranges from 12 to 30%...
  42. ncbi request reprint Identification of a genotoxic mechanism for the carcinogenicity of the environmental pollutant and suspected human carcinogen o-anisidine
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Int J Cancer 116:667-78. 2005
    ..The results, the first report on the potential of the human microsomal CYP enzymes to activate o-anisidine, strongly suggest a carcinogenic potential of this rodent carcinogen for humans...
  43. ncbi request reprint Molecular mechanism of genotoxicity of the environmental pollutant 3-nitrobenzanthrone
    Marie Stiborova
    Department of Biochemistry, Charles University, Albertov 2030, 12840 Prague 2, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 149:191-7. 2005
    ..The results suggest that both CYPs and peroxidases may play an important role in metabolism of 3-ABA to reactive species forming DNA adducts, participating in genotoxicity of this compound and its parental counterpart, 3-NBA...
  44. ncbi request reprint Carcinogenic aristolochic acids upon activation by DT-diaphorase form adducts found in DNA of patients with Chinese herbs nephropathy
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, The Czech Republic
    Carcinogenesis 23:617-25. 2002
    ..The results presented here are the first report demonstrating a reductive activation of carcinogenic AAs by DT-diaphorase...
  45. ncbi request reprint The binding of aristolochic acid I to the active site of human cytochromes P450 1A1 and 1A2 explains their potential to reductively activate this human carcinogen
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, The Czech Republic
    Cancer Lett 229:193-204. 2005
    ..This observation explains the lower reductive potential of CYP1A1 for AAI than CYP1A2, detected experimentally...
  46. ncbi request reprint Human enzymes involved in the metabolic activation of carcinogenic aristolochic acids: evidence for reductive activation by cytochromes P450 1A1 and 1A2
    M Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, The Czech Republic
    Chem Res Toxicol 14:1128-37. 2001
    ..These results are the first report on the activation of AA by human enzymes and clearly demonstrate the role of P450 1A1, 1A2, and NADPH:P450 reductase in catalyzing the reductive activation of AA...
  47. ncbi request reprint Evidence for reductive activation of carcinogenic aristolochic acids by prostaglandin H synthase -- (32)P-postlabeling analysis of DNA adduct formation
    M Stiborova
    Faculty of Science, Department of Biochemistry, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic
    Mutat Res 493:149-60. 2001
    ..The results presented here are the first report demonstrating a reductive activation of nitroaromatic compounds by PHS-1...
  48. ncbi request reprint The anticancer drug ellipticine is a potent inducer of rat cytochromes P450 1A1 and 1A2, thereby modulating its own metabolism
    Dagmar Aimová
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic
    Drug Metab Dispos 35:1926-34. 2007
    ....
  49. ncbi request reprint New selective inhibitors of cytochromes P450 2B and their application to antimutagenesis of tamoxifen
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic
    Arch Biochem Biophys 403:41-9. 2002
    ..2-PMADA and 3-PMDIA are very potent for inhibition of formation of these DNA adducts and warrant consideration as candidates for preventing endometrial cancer development by tamoxifen in humans treated with this anticancer drug...
  50. ncbi request reprint alpha-Naphthoflavone acts as activator and reversible or irreversible inhibitor of rabbit microsomal CYP3A6
    L Boek-Dohalská
    Department of Biochemistry, Faculty of Natural Sciences, Charles University, Albertov 2030, 128 40 2, Prague, Czech Republic
    Chem Biol Interact 138:85-106. 2001
    ..The obtained results strongly suggest that the CYP3A active center contains at least two and probably three distinct binding sites for substrates...
  51. ncbi request reprint The anticancer agent ellipticine on activation by cytochrome P450 forms covalent DNA adducts
    M Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 2, The, Prague, Czech Republic
    Biochem Pharmacol 62:1675-84. 2001
    ..The DNA adduct formation we describe is a novel mechanism for the ellipticine action and might in part explain its tumor specificity...
  52. ncbi request reprint Evidence for activation of carcinogenic o-anisidine by prostaglandin H synthase: 32P-postlabelling analysis of DNA adduct formation
    M Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Gen Physiol Biophys 20:267-79. 2001
    ..The results presented here are the first report demonstrating a PHS-mediated activation of o-anisidine to reactive species forming covalent DNA adducts...
  53. ncbi request reprint Prostaglandin H synthase-medicated oxidation and binding to DNA of a detoxication metabolite of carcinogenic Sudan I, 1-(phenylazo)-2,6-dihydroxynaphthalene
    M Stiborova
    Department of Biochemistry, Faculty of Natural Sciences, Charles University, Prague, Czech Republic
    Cancer Lett 146:53-60. 1999
    ....
  54. ncbi request reprint The role of biotransformation enzymes in the development of renal injury and urothelial cancer caused by aristolochic acid: urgent questions and difficult answers
    Marie Stiborova
    Department of Biochemistry, Faculty of Sciences, Charles University, Albertov 2030, Prague, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 153:5-11. 2009
    ....
  55. ncbi request reprint Carcinogenic pollutants o-nitroanisole and o-anisidine are substrates and inducers of cytochromes P450
    Helena Rýdlová
    Department of Biochemistry, Charles University, Albertov 2030, Prague 2, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 149:441-7. 2005
    ..Furthermore, by induction of rat hepatic and renal CYP1A1/2, both o-nitroanisole and o-anisidine influence their carcinogenic effects, modifying their detoxification and/or activation pathways...
  56. ncbi request reprint Oxidation of xenobiotics by plant microsomes, a reconstituted cytochrome P450 system and peroxidase: a comparative study
    M Stiborova
    Department of Biochemistry, Faculty of Sciences, Charles University, Prague, Czech Republic
    Phytochemistry 54:353-62. 2000
    ..The results demonstrate the progress in resolving the role of plant CYP and peroxidase enzymes in oxidation of xenobiotics...
  57. ncbi request reprint Human cytochromes P450 1A1 and 1A2 participate in detoxication of carcinogenic aristolochic acid
    Jana Sistkova
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 29:733-7. 2008
    ..The aim of the study was to resolve which cytochromes P450 (CYP) detoxicate the major component of AA, aristolochic acid I (AAI), to its O-demethylated metabolite, aristolochic acid Ia (AAIa)...
  58. doi request reprint Redox cycling in the metabolism of the environmental pollutant and suspected human carcinogen o-anisidine by rat and rabbit hepatic microsomes
    Karel Naiman
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic
    Chem Res Toxicol 21:1610-21. 2008
    ..The data demonstrate the participation of different rat and rabbit P450s in o-anisidine metabolism and indicate that both experimental animal species might serve as suitable models to mimic the fate of o-anisidine in human...
  59. ncbi request reprint Enzymes involved in the metabolism of the carcinogen 2-nitroanisole: evidence for its oxidative detoxication by human cytochromes P450
    Marketa Miksanova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, The Czech Republic
    Chem Res Toxicol 17:663-71. 2004
    ..These results, the first report on the metabolism of 2-NA by human P450 enzymes, clearly demonstrate that P450 2E1 is the major human enzyme oxidizing this carcinogen in human liver...
  60. doi request reprint Chemopreventive compounds--view from the other side
    P Hodek
    Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova, Prague 2, Czech Republic
    Chem Biol Interact 180:1-9. 2009
    ..Hence, the predicted chemopreventive potential is not attained in respect of cancer prevention; moreover, the administration of high doses of chemopreventive compounds might be even detrimental for the human health...
  61. ncbi request reprint The binding affinity of carcinogenic N-nitrosodimethylamine and N-nitrosomethylaniline to cytochromes P450 2B4, 2E1 and 3A6 does not dictate the rate of their enzymatic N-demethylation
    Miroslav Sulc
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 43 Prague 2, Czech Republic
    Gen Physiol Biophys 29:175-85. 2010
    ..The results demonstrate that investigations utilizing several enzymatic approaches are necessary to properly evaluate the mechanism and efficiency of NDMA and NMA oxidation by CYPs in vitro...
  62. ncbi request reprint Oxidation of 3-aminobenzanthrone, a human metabolite of carcinogenic environmental pollutant 3-nitrobenzanthrone, by cytochromes P450 - similarity between human and rat enzymes
    Jana Mizerovska
    Department of Biochemistry, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 30:52-9. 2009
    ..Characterization of 3-ABA metabolites formed by rat hepatic microsomes containing cytochromes P450 (CYPs) and identification of the major rat and human CYPs participating in this process are aims of this study...
  63. ncbi request reprint Induction of cytochromes P450 in small intestine by chemopreventive compounds
    Jitka Krizkova
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 29:717-21. 2008
    ..However, they can modulate the activity of xenobiotic-metabolizing enzymes involved in activation and detoxification of food and environmental carcinogens. Thus, their potential negative effects should be examined...
  64. ncbi request reprint Oxidative detoxication of carcinogenic 2-nitroanisole by human, rat and rabbit cytochrome P450
    Helena Dracinska
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 27:9-13. 2006
    ..Comparison between P450s of experimental animals and humans is essential for the extrapolation of animal carcinogenicity data to the human situation and to assess health risk...
  65. ncbi request reprint Cytochromes P450 reconstituted with NADPH: P450 reductase mimic the activating and detoxicating metabolism of the anticancer drug ellipticine in microsomes
    Vera Kotrbova
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 27:18-22. 2006
    ..Recently, we found that after cytochrome P450 (CYP)-mediated oxidation ellipticine forms covalent DNA adducts. Ellipticine oxidation by isolated CYP and its binding to DNA is the target of this study...
  66. ncbi request reprint Cytotoxicity of and DNA adduct formation by ellipticine in human U87MG glioblastoma cancer cells
    Eva Martinkova
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 30:60-6. 2009
    ..The toxicity of ellipticine to U87MG glioblastoma cells and mechanisms of its action to these cells are aims of this study...
  67. ncbi request reprint Rat cytochromes P450 oxidize 2-nitrophenol, a human metabolite of carcinogenic 2-nitroanisole
    Martina Svobodova
    Department of Biochemistry, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 30:46-51. 2009
    ..Characterization of the products of 2-NP metabolism by rat hepatic microsomes containing cytochromes P450 (CYPs) and identification of the major CYP enzymes participating in this process are aims of this study...
  68. ncbi request reprint A one-electron oxidation of carcinogenic nonaminoazo dye Sudan I by horseradish peroxidase
    Marcela Semanska
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 29:712-6. 2008
    ..The aim of the study was to examine oxidation of carcinogenic Sudan I by peroxidase and characterize the structure of its two major peroxidasemediated metabolites. Another target of the study was to evaluate a mechanism of this oxidation...
  69. ncbi request reprint Structural analysis of binding of a diamantoid substrate to cytochrome P450 2B4: possible role of Arg 133 in modulation of function and activity of this enzyme
    Miroslav Sulc
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 29:722-7. 2008
    ..Understanding the enzyme mechanism of P450 enzymes needs a detailed knowledge of substrate-enzyme interactions. Here, we examined the interaction of cytochrome P450 2B4 with a diamantanoid substrate...
  70. ncbi request reprint An investigation of the metabolism of N-nitrosodimethylamine and N-nitrosomethylaniline by horseradish peroxidase in vitro
    M Stiborova
    Department of Biochemistry, Faculty of Natural Sciences, Charles University, Prague, Czech Republic
    Gen Physiol Biophys 16:285-97. 1997
    ..The results are discussed from the point of view of the role of peroxidases in the metabolism of N-nitrosamines...
  71. ncbi request reprint Modulation of cytochrome P450 enzyme system by selected flavonoids
    Petr Hodek
    Department of Biochemistry, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 30:67-71. 2009
    ....
  72. doi request reprint The mechanism of cytotoxicity and DNA adduct formation by the anticancer drug ellipticine in human neuroblastoma cells
    Jitka Poljakova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic
    Biochem Pharmacol 77:1466-79. 2009
    ..The results demonstrate that among the multiple modes of ellipticine antitumor action, formation of covalent DNA adducts by ellipticine is the predominant mechanism of cytotoxicity to IMR-32 and UKF-NB-4 neuroblastoma cells...
  73. ncbi request reprint Structural requirements for inhibitors of cytochromes P450 2B: assessment of the enzyme interaction with diamondoids
    Petr Hodek
    Department of Biochemistry, Faculty of Science, Charles University, Hlavova 2030, Prague 2 CZ 12840, Czech Republic
    J Enzyme Inhib Med Chem 20:25-33. 2005
    ..9A inner diameter, 7.9A length) forming an angle of approximately 43 degrees with the heme plane. CYP2B specific diamondoids, namely 3-IPMDIA, showing the highest binding affinity, should be considered for a potential clinical use...
  74. ncbi request reprint Toxicological aspects of flavonoid interaction with biomacromolecules
    Petr Hodek
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 27:14-7. 2006
    ..Thus, their potential harmful effect on the human body should be examined in detail...
  75. ncbi request reprint Flavonoids-potent and versatile biologically active compounds interacting with cytochromes P450
    Petr Hodek
    Department of Biochemistry, Charles University in Prague, Hlavova 2030, CZ 128 40 Prague, Czech Republic
    Chem Biol Interact 139:1-21. 2002
    ..For these reasons the structure-function relationship of flavonoids is extensively studied to provide an inspiration for a rational drug and/or chemopreventive agent design of future pharmaceuticals...
  76. ncbi request reprint Mapping of cytochrome P450 2B4 substrate binding sites by photolabile probe 3-azidiamantane: identification of putative substrate access regions
    Petr Hodek
    Department of Biochemistry, Faculty of Science, Charles University in Prague, Albertov 2030, 128 40 Prague, Czech Republic
    Arch Biochem Biophys 468:82-91. 2007
    ..In addition to crystal structures of CYP2B4 chimeras and molecular dynamics simulations, our data of photoaffinity labeling of the full CYP2B4 molecule provide further insight into functional and structural aspects of substrate binding...
  77. ncbi request reprint Mechanism of peroxidase-mediated oxidation of carcinogenic o-anisidine and its binding to DNA
    Marie Stiborova
    Department of Biochemistry, Faculty of Natural Sciences, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic
    Mutat Res 500:49-66. 2002
    ..The limitation of the 32P-postlabeling technique to analyze DNA adducts derived from o-anisidine as a means to estimate its genotoxicity is discussed...
  78. ncbi request reprint Preparation of apo-cytochrome b5 utilizing heme transfer to apo-myoglobin
    Barbora Mrazova
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 30:72-9. 2009
    ..To prepare apo-cyt b5, heme transfer from native cyt b5 to a protein with higher affinity toward the heme, the horse heart apo-myoglobin, was utilized...
  79. ncbi request reprint Isolation and partial characterization of the adduct formed by 13-hydroxyellipticine with deoxyguanosine in DNA
    Michaela Moserova
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 29:728-32. 2008
    ..The development of the methods suitable for the preparation of this adduct in the amounts sufficient for identification of its structure and those for its isolation and partial characterization is the aim of this study...
  80. ncbi request reprint Macleaya cordata extract and Sangrovit genotoxicity. Assessment in vivo
    Marie Stiborova
    Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 152:35-9. 2008
    ..cordata extract and powdered M. cordata) in a 90-day pilot study...
  81. ncbi request reprint Antitumor drug ellipticine inhibits the activities of rat hepatic cytochromes P450
    Dagmar Aimová
    Department of Biochemistry, Charles University, Albertov 2030, Prague 2, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 149:437-40. 2005
    ..The results indicate that inhibition of CYPs by ellipticine cannot be explained only by its differential potency to bind to individual CYPs...
  82. ncbi request reprint Continuous aerobic phenol degradation by defined mixed immobilized culture in packed bed reactors
    J Paca
    Department of Biochemistry, Faculty of Science, Charles University, 128 40 Prague 2, Czechia
    Folia Microbiol (Praha) 50:301-8. 2005
    ..2-4.0 and no incompletely oxidized metabolic intermediates were found. The free cell concentration in the effluent increased after the phenol overloading time period...
  83. doi request reprint Preparation of a biologically active apo-cytochrome b5 via heterologous expression in Escherichia coli
    Vera Kotrbova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 43 Prague 2, Czech Republic
    Protein Expr Purif 66:203-9. 2009
    ..The reconstituted apo-b(5) is also fully biologically active, exhibiting the comparable stimulation effect on the CYP3A4 enzymatic activity towards oxidation of 1-phenylazo-2-hydroxynaphthalene (Sudan I) as native rabbit and human b(5)...
  84. ncbi request reprint Oxidation of an antitumor drug ellipticine by peroxidases
    Jitka Poljakova
    Department of Biochemistry, Charles University, Albertov 2030, Prague 2, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 149:449-53. 2005
    ..The implication of the oxidation of ellipticine by peroxidases in its mechanism of action is discussed...
  85. ncbi request reprint DNA adduct formation by the anticancer drug ellipticine in human leukemia HL-60 and CCRF-CEM cells
    Jitka Poljakova
    Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic
    Cancer Lett 252:270-9. 2007
    ..The results suggest the adduct formation as a new mode of antitumor action of ellipticine for leukemia...
  86. ncbi request reprint Isolation and partial characterization of catechol 1,2-dioxygenase of phenol degrading yeast Candida tropicalis
    Lenka Vilimkova
    Department of Biochemistry, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 30:80-7. 2009
    ..The development of the procedure suitable for catechol 1,2-dioxygenase isolation and partial characterization of this enzyme are the aims of this study...