Karel Smetana

Summary

Affiliation: Charles University
Country: Czech Republic

Publications

  1. ncbi request reprint Fusion of macrophages on an implant surface is associated with down-regulated expression of ligands for galectin-1 and -3 in the rat
    K Smetana
    Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic
    Biomaterials 19:1799-805. 1998
  2. ncbi request reprint Coexpression of binding sites for A(B) histo-blood group trisaccharides with galectin-3 and Lag antigen in human Langerhans cells
    K Smetana
    Institute for Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic
    J Leukoc Biol 66:644-9. 1999
  3. ncbi request reprint Experimental lesion of medial frontal cortex mediates inhibition of expression of carbohydrate-binding sites in the spleen macrophages in rat
    K Smetana
    Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic
    Folia Biol (Praha) 45:87-90. 1999
  4. ncbi request reprint Synthetic hydrogel capacity to induce formation of foreign-body giant multinucleate cells differs in vivo and in vitro
    K Smetana
    Charles University, 1st Faculty of Medicine, Institute of Anatomy, Prague, Czech Republic
    Folia Biol (Praha) 46:113-8. 2000
  5. ncbi request reprint Diagnostic relevance of Langerin detection in cells from bronchoalveolar lavage of patients with pulmonary Langerhans cell histiocytosis, sarcoidosis and idiopathic pulmonary fibrosis
    Karel Smetana
    Institute of Anatomy, Charles University, 1st Faculty of Medicine, Unemocnice 3, 128 00, Prague 2, Czech Republic
    Virchows Arch 444:171-4. 2004
  6. doi request reprint Loss of adhesion/growth-regulatory galectin-9 from squamous cell epithelium in head and neck carcinomas
    Zdeněk Fík
    Institute of Anatomy, 1st Faculty of Medicine, Charles University, U nemocnice, Prague, Czech Republic
    J Oral Pathol Med 42:166-73. 2013
  7. ncbi request reprint Correlation of expression of nuclear proteins pKi67 and p63 with lectin histochemical features in head and neck squamous cell cancer
    Martin Chovanec
    1st Faculty of Medicine, Institute of Anatomy, Charles University, Prague, Czech Republic
    Int J Oncol 27:409-15. 2005
  8. ncbi request reprint Defining the glycophenotype of squamous epithelia using plant and mammalian lectins. Differentiation-dependent expression of alpha2,6- and alpha2,3-linked N-acetylneuraminic acid in squamous epithelia and carcinomas, and its differential effect on binding
    Zuzana Holíková
    Charles University, 1st Faculty of Medicine, Institute of Anatomy, Prague, Czech Republic
    APMIS 110:845-56. 2002
  9. ncbi request reprint Nuclear presence of adhesion-/growth-regulatory galectins in normal/malignant cells of squamous epithelial origin
    Karel Smetana
    Institute of Anatomy, 1st Faculty of Medicine, Charles University, U nemocnice 3, 128 00, Prague 2, Czech Republic
    Histochem Cell Biol 125:171-82. 2006
  10. doi request reprint Mouse 3T3 fibroblasts under the influence of fibroblasts isolated from stroma of human basal cell carcinoma acquire properties of multipotent stem cells
    Pavol Szabo
    Institute of Anatomy, First Faculty of Medicine, Charles University in Prague, U nemocnice 3, CZ 12800 Prague 2, Czech Republic
    Biol Cell 103:233-48. 2011

Collaborators

Detail Information

Publications61

  1. ncbi request reprint Fusion of macrophages on an implant surface is associated with down-regulated expression of ligands for galectin-1 and -3 in the rat
    K Smetana
    Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic
    Biomaterials 19:1799-805. 1998
    ....
  2. ncbi request reprint Coexpression of binding sites for A(B) histo-blood group trisaccharides with galectin-3 and Lag antigen in human Langerhans cells
    K Smetana
    Institute for Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic
    J Leukoc Biol 66:644-9. 1999
    ..These results suggest that galectin-3 is present in Birbeck granules, where it retains reactivity for its glycoligands...
  3. ncbi request reprint Experimental lesion of medial frontal cortex mediates inhibition of expression of carbohydrate-binding sites in the spleen macrophages in rat
    K Smetana
    Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic
    Folia Biol (Praha) 45:87-90. 1999
    ..These data intimate that distinct brain regions are involved in the control of the phenotype of macrophages in the central lymphoid organs by currently elusive biochemical mechanisms...
  4. ncbi request reprint Synthetic hydrogel capacity to induce formation of foreign-body giant multinucleate cells differs in vivo and in vitro
    K Smetana
    Charles University, 1st Faculty of Medicine, Institute of Anatomy, Prague, Czech Republic
    Folia Biol (Praha) 46:113-8. 2000
    ..They also indicate potential differences between granuloma formation induced by parasites and by foreign bodies...
  5. ncbi request reprint Diagnostic relevance of Langerin detection in cells from bronchoalveolar lavage of patients with pulmonary Langerhans cell histiocytosis, sarcoidosis and idiopathic pulmonary fibrosis
    Karel Smetana
    Institute of Anatomy, Charles University, 1st Faculty of Medicine, Unemocnice 3, 128 00, Prague 2, Czech Republic
    Virchows Arch 444:171-4. 2004
    ..These results provide evidence for the usefulness of Langerin-directed immuno- and glycohistochemical monitoring of bronchoalveolar lavage fluid in the diagnosis of pulmonary Langerhans cell histiocytosis...
  6. doi request reprint Loss of adhesion/growth-regulatory galectin-9 from squamous cell epithelium in head and neck carcinomas
    Zdeněk Fík
    Institute of Anatomy, 1st Faculty of Medicine, Charles University, U nemocnice, Prague, Czech Republic
    J Oral Pathol Med 42:166-73. 2013
    ..This lectin can represent a potential marker of normality in the cases of the studied squamous cell epithelia...
  7. ncbi request reprint Correlation of expression of nuclear proteins pKi67 and p63 with lectin histochemical features in head and neck squamous cell cancer
    Martin Chovanec
    1st Faculty of Medicine, Institute of Anatomy, Charles University, Prague, Czech Republic
    Int J Oncol 27:409-15. 2005
    ..Based on the concept of the sugar code further analysis of cell characteristics such as proliferation together with lectin histochemical features, especially using tissue lectins as probes, is thus warranted...
  8. ncbi request reprint Defining the glycophenotype of squamous epithelia using plant and mammalian lectins. Differentiation-dependent expression of alpha2,6- and alpha2,3-linked N-acetylneuraminic acid in squamous epithelia and carcinomas, and its differential effect on binding
    Zuzana Holíková
    Charles University, 1st Faculty of Medicine, Institute of Anatomy, Prague, Czech Republic
    APMIS 110:845-56. 2002
    ....
  9. ncbi request reprint Nuclear presence of adhesion-/growth-regulatory galectins in normal/malignant cells of squamous epithelial origin
    Karel Smetana
    Institute of Anatomy, 1st Faculty of Medicine, Charles University, U nemocnice 3, 128 00, Prague 2, Czech Republic
    Histochem Cell Biol 125:171-82. 2006
    ..Cytochemical expression profiling is a prerequisite for the selection of distinct proteins for targeted modulation of gene expression as a step toward functional analysis...
  10. doi request reprint Mouse 3T3 fibroblasts under the influence of fibroblasts isolated from stroma of human basal cell carcinoma acquire properties of multipotent stem cells
    Pavol Szabo
    Institute of Anatomy, First Faculty of Medicine, Charles University in Prague, U nemocnice 3, CZ 12800 Prague 2, Czech Republic
    Biol Cell 103:233-48. 2011
    ....
  11. ncbi request reprint Nucleostemin expression in squamous cell carcinoma of the head and neck
    Zdenek Cada
    Institute of Anatomy and Head and Neck Surgery, First Faculty of Medicine, Second Faculty of Medicine, Charles University, Prague, Czech Republic
    Anticancer Res 27:3279-84. 2007
    ..This study presents initial data on presence of nucleostemin--a nucleolar protein typical of stem cells in the normal squamous epithelium of the oropharynx and larynx - in squamous cell carcinoma originating from these epithelia...
  12. ncbi request reprint Immunocyto- and histochemical profiling of nucleostemin expression: marker of epidermal stem cells?
    Lukas Lacina
    Charles University, 1st Faculty of Medicine, Institute of Anatomy, Prague, Czech Republic
    J Dermatol Sci 44:73-80. 2006
    ..In addition, probing the value of this protein as a marker of epidermal stem cells was an aim of this study...
  13. ncbi request reprint Analysis of binding of mannosides in relation to Langerin (CD207) in Langerhans cells of normal and transformed epithelia
    Jan Plzak
    Institute of Anatomy, Department of Otorhinolaryngology, Head and Neck Surgery, 1st Faculty of Medicine, Charles University, Prague, Czech Republic
    Histochem J 34:247-53. 2002
    ....
  14. ncbi request reprint Comparative analysis of alpha2,3/2,6-linked N-acetylneuraminic acid and cytokeratin expression in head and neck squamous cell carcinoma
    Martin Chovanec
    First Faculty of Medicine, Institute of Anatomy, Charles University, 128 00 Prague, Czech Republic
    Oncol Rep 12:297-301. 2004
    ..Monitoring HNSCC using multiparameter phenotype analysis can produce new data important for the understanding of the biological behavior of HNSCC, and useful for the treatment rationalization...
  15. ncbi request reprint Glycobiology of head and neck squamous epithelia and carcinomas
    Jan Plzak
    Department of Otorhinolaryngology and Head and Neck Surgery, Charles University, Prague, Czech Republic
    ORL J Otorhinolaryngol Relat Spec 67:61-9. 2005
    ..Finally, the use of the described data in the diagnosis and prospectively in the treatment of head and neck squamous cell carcinoma is shown...
  16. ncbi request reprint Transient expression of keratin 19 is induced in originally negative interfollicular epidermal cells by adhesion of suspended cells
    Barbora Dvořánková
    Institute of Anatomy, 1st Faculty of Medicine, Charles University, U nemocnice 3, 128 00 Prague 2, Czech Republic
    Int J Mol Med 16:525-31. 2005
    ..In conclusion, the expression of poor levels of differentiation induced by cell adhesion is transient. Also, keratin 19 expression should not be exclusively regarded as a marker of stem cell activity...
  17. doi request reprint Upregulation of IL-6, IL-8 and CXCL-1 production in dermal fibroblasts by normal/malignant epithelial cells in vitro: Immunohistochemical and transcriptomic analyses
    Michal Kolar
    Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics, Academy of Science of the Czech Republic, Prague, Czech Republic
    Biol Cell 104:738-51. 2012
    ..Considering an analogy between wound healing and tumour progression, we studied chemokine and cytokine transcription and expression in normal fibroblasts by co-culture and in situ...
  18. ncbi request reprint Marker profiling of normal keratinocytes identifies the stroma from squamous cell carcinoma of the oral cavity as a modulatory microenvironment in co-culture
    Lukas Lacina
    First Faculty of Medicine, Institute of Anatomy, Charles University in Prague, Prague, Czech Republic
    Int J Radiat Biol 83:837-48. 2007
    ..We address this issue for keratinocytes using tumor-derived stromal cells in a co-culture system...
  19. ncbi request reprint Galectin-3, an endogenous lectin, as a tool for monitoring cell differentiation in head and neck carcinomas with implications for lectin-glycan functionality
    Jan Betka
    Department of Otorhinolaryngology and Head and Neck Surgery, 1st Faculty of Medicine, Faculty Hospital Motol, Prague, Czech Republic
    Acta Otolaryngol 123:261-3. 2003
    ..The aim of this study was to monitor the carbohydrate-dependent binding of labeled galectin-3 to primary head and neck squamous cell carcinomas (from the tonsil, base of the tongue and larynx) and lymph node metastases...
  20. ncbi request reprint Dolichos biflorus agglutinin-binding site expression in basal keratinocytes is associated with cell differentiation
    Barbora Dvořánková
    Charles University, 3rd Faculty of Medicine, Department of Burn Surgery, Prague, Czech Republic
    Biol Cell 94:365-73. 2002
    ..Some role of DBA-reactive glycoligand expressions in a suprabasal movement of differentiated basal cells can be hypothesized...
  21. doi request reprint Cultivation-dependent plasticity of melanoma phenotype
    Ondřej Kodet
    1st Faculty of Medicine, Department of Dermatovenereology, Charles University, Prague, Czech Republic
    Tumour Biol 34:3345-55. 2013
    ..The presented study shows how the conditions of cultivation of melanoma cells can influence their phenotype. This observation can have some impact on considerations about the role of microenvironment in tumor biology. ..
  22. ncbi request reprint Craniopharyngioma: a case report and comparative galectin histochemical analysis
    Jan Plzak
    1st Faculty of Medicine, Institute of Anatomy, Charles University, Prague, Czech Republic
    Histochem J 34:117-22. 2002
    ....
  23. doi request reprint Context-dependent multifunctionality of galectin-1: a challenge for defining the lectin as therapeutic target
    Karel Smetana
    Institute of Anatomy, 1st Faculty of Medicine, Charles University, U nemocnice 3, 128 00 Prague, Czech Republic
    Expert Opin Ther Targets 17:379-92. 2013
    ..A family of endogenous lectins, sharing folding, a distinct sequence signature and affinity for β-galactosides (thus termed galectins), does so effectively in a context-dependent manner...
  24. ncbi request reprint Galectin-3 - an emerging prognostic indicator in advanced head and neck carcinoma
    Jan Plzak
    Department of Otorhinolaryngology and Head and Neck Surgery, 1st Faculty of Medicine, Charles University, Faculty Hospital Motol, V Uvalu 84, 150 06 Prague 5, Czech Republic
    Eur J Cancer 40:2324-30. 2004
    ..Of note, histochemical application of an endogenous effector after its purification and labelling may bear relevance beyond the galectins...
  25. ncbi request reprint Human hair follicle and interfollicular keratinocyte reactivity to mouse HPV16-transformed cells: an in vitro study
    Karel Smetana
    Charles University, First Faculty of Medicine, Institute of Anatomy, 128 00 Prague 2, Czech Republic
    Oncol Rep 20:75-80. 2008
    ..This observation is compatible with the hypothesis that stromal cells play an important role in tumor progression and spreading...
  26. ncbi request reprint To the intranucleolar translocation of AgNORs in leukemic early granulocytic and plasmacytic precursors
    Karel Smetana
    Institute of Hematology and Blood Transfusion, U Nemocnice 1, Prague 2, 128 20, Czech Republic
    Histochem Cell Biol 125:165-70. 2006
    ..However, more studies in this direction are required in the future...
  27. doi request reprint Head and neck squamous cancer stromal fibroblasts produce growth factors influencing phenotype of normal human keratinocytes
    Hynek Strnad
    Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Histochem Cell Biol 133:201-11. 2010
    ..This hypothesis was also verified by inhibition experiments where blocking antibodies were employed in the medium conditioned by cancer-associated fibroblast. Presence of these growth factors was also detected in tumor samples...
  28. doi request reprint Nucleolar and cytoplasmic RNA density-concentration in leukemia granulocytic progenitors in human bone marrow biopsies: A short cytochemical note
    Karel Smetana
    Institute of Hematology and Blood Transfusion, Prague, Czech Republic
    Acta Histochem 113:58-61. 2011
    ..On the other hand, it should be pointed out that late differentiation stages of granulocytes, starting with myelocytes, possessed nucleolar bodies (nucleoli without surrounding perinucleolar chromatin) of a markedly reduced size...
  29. doi request reprint Smooth muscle actin-expressing stromal fibroblasts in head and neck squamous cell carcinoma: increased expression of galectin-1 and induction of poor prognosis factors
    Jaroslav Valach
    Department of Stomatology, First Faculty of Medicine, Charles University, Prague, Czech Republic
    Int J Cancer 131:2499-508. 2012
    ..These results provide new insights into the significance of presence of myofibroblasts in squamous cell carcinoma...
  30. doi request reprint Human galectins induce conversion of dermal fibroblasts into myofibroblasts and production of extracellular matrix: potential application in tissue engineering and wound repair
    Barbora Dvořánková
    Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic
    Cells Tissues Organs 194:469-80. 2011
    ..In conclusion, we describe the differential potential of certain human galectins to induce the conversion of dermal fibroblasts into MFBs and the generation of a bioactive cell culture substratum...
  31. ncbi request reprint Intercellular crosstalk in human malignant melanoma
    Barbora Dvořánková
    Institute of Anatomy, Charles University, 1st Faculty of Medicine, U nemocnice 3, 128 00, Prague, Czech Republic
    Protoplasma . 2016
    ..This concept is directly applicable to the malignant melanoma. Our review article focuses on possible strategies to modify the intercellular crosstalk in melanoma that can be employed for therapeutic purposes...
  32. ncbi request reprint New aspects of galectin functionality in nuclei of cultured bone marrow stromal and epidermal cells: biotinylated galectins as tool to detect specific binding sites
    Tereza Purkrábková
    Charles University, 1st Faculty of Medicine, Clinical Department of Hematology and Nephrology, 128 08 Prague 2, Czech Republic
    Biol Cell 95:535-45. 2003
    ..In conclusion, the application of labeled galectins to localize accessible binding sites adds a new aspect to the functional analysis of these lectins in the nucleus...
  33. ncbi request reprint The effect of 5-aminolevulinic acid-based photodynamic treatment (PDT) on nucleoli of leukemic granulocytic precursors represented by K562 blastic cells in vitro
    Karel Smetana
    Institute of Hematology and Blood Transfusion, Prague, Czech Republic
    Med Sci Monit 10:BR405-9. 2004
    ....
  34. pmc Melanoma cells influence the differentiation pattern of human epidermal keratinocytes
    Ondřej Kodet
    1st Faculty of Medicine, Institute of Anatomy, Charles University in Prague, U nemocnice 3, CZ 12800 Prague, Czech Republic
    Mol Cancer 14:1. 2015
    ..Presence of MC is as an important modulator of the tumor microenvironment and we compare it to the effect of nonmalignant lowly differentiated cells also originating from neural crest (NCSC)...
  35. ncbi request reprint Mammalian lectin as tool in glycochemistry and histochemistry with relevance for diagnostic procedure
    Karel Smetana
    Charles University, 1st Faculty of Medicine, Institute of Anatomy, U nemocnice, Czech Republic
    Methods Mol Biol 418:171-86. 2008
    ..Position of glycocode and endogenous lectins in squamous epithelium biology under physiological condition and after the malignant transformation is shown as example of the employment of mentioned probes in research and diagnostics...
  36. ncbi request reprint Mean diameter of nucleolar bodies in cultured human leukemic myeloblasts is mainly related to the S and G2 phase of the cell cycle
    Karel Smetana
    Institute of Hematology and Blood Transfusion, U Nemocnice 1, Prague 2, Czech Republic, 128 20
    Eur J Histochem 51:269-74. 2007
    ..Thus, MDNoBs in leukemic myeloblasts might be a helpful tool to estimate the incidence of cells in the S+G2 phase at the single cell level in smear preparations when the number of cells is very small...
  37. ncbi request reprint Epithelial-stromal interaction in squamous cell epithelium-derived tumors: an important new player in the control of tumor biological properties
    Jan Plzak
    Department of Otorhinolaryngology, Head and Neck Surgery, Institute of Anatomy, Center of Cell Therapy and Tissue Repair, Charles University in Prague, Prague, Czech Republic
    Anticancer Res 30:455-62. 2010
    ..We propose these stromal cells and their products as novel targets for anticancer therapy...
  38. pmc Functional differences between neonatal and adult fibroblasts and keratinocytes: Donor age affects epithelial-mesenchymal crosstalk in vitro
    Rosana Mateu
    Institute of Anatomy, First Faculty of Medicine, Charles University, Prague, Czech Republic
    Int J Mol Med 38:1063-74. 2016
    ..These results indicate the important differences between neonatal and adult cells that may be associated with improved wound healing during the early neonatal period. ..
  39. pmc Cancer Microenvironment: What Can We Learn from the Stem Cell Niche
    Lukas Lacina
    Institute of Anatomy, 1st Faculty of Medicine, Charles University, U nemocnice 3, 12800 Prague 2, Czech Republic
    Int J Mol Sci 16:24094-110. 2015
    ..The therapeutic consequences of targeting of microenvironment in anticancer therapy and for improved wound healing are included to article. ..
  40. ncbi request reprint Emerging role of tissue lectins as microenvironmental effectors in tumors and wounds
    Karel Smetana
    Charles University, 1st Faculty of Medicine, Institute of Anatomy, Prague, Czech Republic
    Histol Histopathol 30:293-309. 2015
    ..Such insights are assumed to have potential to contribute to rationally manipulate stem/precursor cells as resource in regenerative medicine. ..
  41. pmc Microarray analysis of serum mRNA in patients with head and neck squamous cell carcinoma at whole-genome scale
    Markéta Capková
    Department of Otorhinolaryngology and Head and Neck Surgery, 1st Faculty of Medicine, Charles University, University Hospital Motol, Institute of Postgraduate Medicine, V Uvalu 84, 15006 Prague 5, Czech Republic Academy of Sciences of the Czech Republic, Institute of Molecular Genetics, Department of Genomics and Bioinformatics, Videnska 1083, 14220 Prague 4, Czech Republic
    Biomed Res Int 2014:408683. 2014
    ....
  42. ncbi request reprint Cultivation of human keratinocytes without feeder cells on polymer carriers containing ethoxyethyl methacrylate: in vitro study
    Jiri Vacik
    Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    J Mater Sci Mater Med 19:883-8. 2008
    ..Keratinocytes cultured on these surfaces are able to migrate to the model wound bed in vitro, where they form distinct colonies and have a normal differentiation potential...
  43. doi request reprint Synthetic polyamine BPA-C8 inhibits TGF-β1-mediated conversion of human dermal fibroblast to myofibroblasts and establishment of galectin-1-rich extracellular matrix in vitro
    Alžběta Mifková
    Institute of Anatomy, Charles University, 1st Faculty of Medicine, U nemocnice 3, 128 00 Prague Czech Republic Department of Otorhinolaryngology and Head and Neck Surgery, Charles University, 1st Faculty of Medicine, V Úvalu 5, 150 00 Prague Czech Republic
    Chembiochem 15:1465-70. 2014
    ..The reported data thus extend current insights into polyamine activity, adding interference with tumor progression to the tumor-promoting processes warranting study. ..
  44. ncbi request reprint Mannosides as crucial part of bioactive supports for cultivation of human epidermal keratinocytes without feeder cells
    Jirí Labský
    Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Biomaterials 24:863-72. 2003
    ..In conclusion, we suggest that these polymers are bioactive offering a perspective for keratinocyte cultivation without feeder cells...
  45. ncbi request reprint The role of dendritic cells in the pharynx
    Jan Plzak
    Department of Otorhinolaryngology and Head and Neck Surgery, First Faculty of Medicine, Charles University, Postgraduate Medical School, Faculty Hospital Motol, V Uvalu 84, 150 06 Prague 5, Czech Republic
    Eur Arch Otorhinolaryngol 260:266-72. 2003
    ..The regulation of immune responses in tonsils by dendritic cells is discussed. Their importance in some disease states is also mentioned...
  46. doi request reprint Regulatory Impact of Amniotic Membrane Transplantation on Presence of Adhesion/Growth-Regulatory Galectins-1 and -7 in Corneal Explants from Acanthamoeba Keratitis Patients: Clinical Note
    Natalia Smorodinova
    a First Faculty of Medicine, Institute of Histology and Embryology, Charles University in Prague, Albertov, Prague, Czech Republic
    Curr Eye Res 41:740-6. 2016
    ..To assess the impact of Acanthamoeba keratitis (AK) and amniotic membrane transplantation (AMT) in corneal explants on presence of two multifunctional endogenous lectins, i.e. galectins-1 and -7...
  47. ncbi request reprint Ageing as an Important Risk Factor for Cancer
    Karel Smetana
    Institute of Anatomy, First Faculty of Medicine, Charles University, Prague, Czech Republic
    Anticancer Res 36:5009-5017. 2016
    ..In this context, the role of cancer stroma in controlling multiple biological properties of tumors is a prospective target for translational research with potential therapeutic outcomes...
  48. doi request reprint Simultaneous blocking of IL-6 and IL-8 is sufficient to fully inhibit CAF-induced human melanoma cell invasiveness
    Njainday Pulo Jobe
    Department of Cell Biology, Charles University in Prague, Vinicna 7, Prague, Czech Republic
    Histochem Cell Biol 146:205-17. 2016
    ..In summary, these experiments indicate the important role of CAFs and IL-8 and IL-6 cytokines in melanoma cell invasiveness. ..
  49. ncbi request reprint [Cancer microenvironment affects biological properties of tumor cells--tumor as an embryologic problem?]
    Karel Smetana
    1 lékarská fakulta, Anatomický ustav, Univerzita Karlova v Praze
    Cas Lek Cesk 149:572-5. 2010
    ..The manipulation of cancer microenvironment represents the perspective therapeutic tool for cancer treatment in future...
  50. doi request reprint Cancer-associated fibroblasts are not formed from cancer cells by epithelial-to-mesenchymal transition in nu/nu mice
    Barbora Dvořánková
    First Faculty of Medicine, Institute of Anatomy, Charles University, U nemocnice 3, 128 00, Prague 2, Czech Republic
    Histochem Cell Biol 143:463-9. 2015
    ..These results suggest that cancer-associated stromal fibroblasts are not formed by epithelial-to-mesenchymal transition from cancer cells...
  51. ncbi request reprint A short note on the nuclear diameter in human early granulocytic progenitors
    Karel Smetana
    Institute of Haematology and Blood Transfusion, Prague, Czech Republic
    Hematology 11:399-401. 2006
    ..In addition, the results of the present study also indicated that the nuclear diameter in myeloblasts as well as promyelocytes of studied patients was not substantially influenced by the cytostatic therapy with imatinib mesylate...
  52. ncbi request reprint Differentiation-dependent glycosylation of cells in squamous cell epithelia detected by a mammalian lectin
    Jan Plzak
    Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic
    Cells Tissues Organs 171:135-44. 2002
    ..Further application of tissue lectins for characterizing ligand expression and its modulation is an important step to reveal functional relevance...
  53. doi request reprint Effect of cancer-associated fibroblasts on the migration of glioma cells in vitro
    Jana Trylcova
    Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University in Prague, U nemocnice 5, 128 53, Prague 2, Czech Republic
    Tumour Biol 36:5873-9. 2015
    ..These observations indicate the potential role of CAF-like cells in glioblastoma biology...
  54. doi request reprint Valproic acid triggers differentiation and apoptosis in AML1/ETO-positive leukemic cells specifically
    Michal Zapotocky
    CLIP, Department of Pediatric Hematology and Oncology, Charles University, 2nd Medical School, Prague, Czech Republic
    Cancer Lett 319:144-53. 2012
    ..Our data suggest that AML1/ETO-positive patients might derive the greatest benefit from VPA treatment...
  55. ncbi request reprint A short note on the nucleolar size and density in apoptotic leukemic granulocytic precursors (HL-60 cells)
    Karel Smetana
    Institute of Hematology and Blood Transfusion, Prague, Czech Republic
    Life Sci 75:791-6. 2004
    ..Thus, large and dense nucleoli in apoptotic cells are not necessarily related to the nucleolar biosynthetic or cell proliferation activity...
  56. doi request reprint On the nucleolar and cytoplasmic RNA density during "cell dedifferentiation" represented by blastic transformation of human mature T lymphocytes - a cytochemical study
    Karel Smetana
    Institute of Hematology and Blood Transfusion, Prague, Czech Republic
    Folia Histochem Cytobiol 46:429-32. 2008
    ..Such difference suggested that the RNA content in nucleoli was related mainly to their size...
  57. ncbi request reprint Comparative phenotypic characterization of keratinocytes originating from hair follicles
    Jiri Klima
    Institute of Animal Physiology and Genetics, Academy of Sciences, Libechov, Czech Republic
    J Mol Histol 36:89-96. 2005
    ..It can be considered a potential marker of a distinct cell population, probably at the beginning of a differentiation cascade of keratinocytes...
  58. ncbi request reprint Role of the carbohydrate recognition domains of mouse galectin-4 in oligosaccharide binding and epitope recognition and expression of galectin-4 and galectin-6 in mouse cells and tissues
    Vladimíra Marková
    Laboratory of Molecular Glycobiology, Department of Recombinant Expression and Structural Biology, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic
    Int J Mol Med 18:65-76. 2006
    ..Interestingly, mGalectin-4, but not CRD forms, binds to the suprabasal layer of squamous epithelium of the tongue, suggesting that the link region also plays an important role in ligand recognition...
  59. ncbi request reprint The miniature pig as an animal model in biomedical research
    Petr Vodicka
    Institute of Animal Physiology and Genetics, Department of Physiology of Reproduction, Rumburska 89, Libechov 277 21, Czech Republic
    Ann N Y Acad Sci 1049:161-71. 2005
    ....
  60. ncbi request reprint Reconstruction of epidermis by grafting of keratinocytes cultured on polymer support--clinical study
    Barbora Dvořánková
    Prague Burn Center, 3rd Faculty of Medicine, Center of Cell Therapy and Tissue Repair, Czech Republic
    Int J Dermatol 42:219-23. 2003
    ..We demonstrate the results of a clinical trial of the grafting of cultured keratinocytes directly on a polymer cultivation support in the treatment of skin defects in seriously burned patients and in patients with trophic ulcers...
  61. ncbi request reprint Intercellular cell adhesion molecule-1 and selectin ligands in acute cardiac allograft rejection: a study on gene-deficient mouse models
    Jiri Lacha
    Department of Nephrology, Transplant Unit, Institute for Clinical and Experimental Medicine, Prague 4, Czech Republic
    J Leukoc Biol 71:311-8. 2002
    ....