Karel Holada

Summary

Affiliation: Charles University
Country: Czech Republic

Publications

  1. ncbi request reprint Expression of cellular prion protein on platelets from patients with gray platelet or Hermansky-Pudlak syndrome and the protein's association with alpha-granules
    Karel Holada
    Institute of Immunology and Microbiology, 1st Faculty of Medicine Charles University Prague, Studnickova 7, 128 20 Prague 2, Czech Republic
    Haematologica 91:1126-9. 2006
  2. ncbi request reprint Divergent expression of cellular prion protein on blood cells of human and nonhuman primates
    Karel Holada
    Department of Immunology and Microbiology, 1st Medical Faculty, Charles University, Prague, Czech Republic
    Transfusion 47:2223-32. 2007
  3. doi request reprint Underestimation of the expression of cellular prion protein on human red blood cells
    Martin Panigaj
    Institute of Immunology and Microbiology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic
    Transfusion 51:1012-21. 2011
  4. doi request reprint Blood storage affects the detection of cellular prion protein on peripheral blood leukocytes and circulating dendritic cells in part by promoting platelet satellitism
    Hana Glier
    Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
    J Immunol Methods 380:65-72. 2012
  5. pmc Development of monoclonal antibodies specific for glycated prion protein
    Eva Dvorakova
    Institute of Immunology and Microbiology, 1st Faculty of Medicine, Charles University Prague, Studnickova 7, Prague 2, Czech Republic
    J Toxicol Environ Health A 74:1469-75. 2011
  6. pmc Expression of prion protein in mouse erythroid progenitors and differentiating murine erythroleukemia cells
    Martin Panigaj
    Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
    PLoS ONE 6:e24599. 2011
  7. doi request reprint Cellular prion protein in blood platelets associates with both lipid rafts and the cytoskeleton
    Adela Brouckova
    Charles University, Institute of Immunology and Microbiology, Prague 2, Czech Republic
    Thromb Haemost 102:966-74. 2009
  8. doi request reprint Photodynamic inactivation of prions by disulfonated hydroxyaluminium phthalocyanine
    Olga Janouskova
    Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague, Studnickova 7, 128 00 Prague 2, Czech Republic
    J Gen Virol 93:2512-7. 2012
  9. ncbi request reprint Reduced erythroid cell and erythropoietin production in response to acute anemia in prion protein-deficient (Prnp-/-) mice
    Jan H Zivny
    1st School of Medicine, Charles University, Prague, Czech Republic
    Blood Cells Mol Dis 40:302-7. 2008
  10. doi request reprint Gerstmann-Sträussler-Scheinker syndrome with the P102L pathogenic mutation presenting as familial Creutzfeldt-Jakob disease: a case report and review of the literature
    Robert Rusina
    Department of Neurology, Thomayer Teaching Hospital and Institute for Postgraduate Education in Medicine, Prague, Czech Republic
    Neurocase 19:41-53. 2013

Collaborators

  • Robert Rusina
  • Paul Brown
  • Monique P Gelderman
  • Martin Panigaj
  • Jan Simak
  • Jaroslav G Vostal
  • Olga Janouskova
  • Hana Glier
  • Eva Dvorakova
  • Adela Brouckova
  • Jan H Zivny
  • Antonio M Risitano
  • Neal S Young
  • Jan Rakusan
  • Marie Karásková
  • Marek Prouza
  • Marcela Wildová
  • Hana Glierova
  • Fei Xu
  • John Piper
  • Jaroslaw P Maciejewski
  • Guibin Chen
  • Jan Janota
  • FELICE D'AGNILLO

Detail Information

Publications15

  1. ncbi request reprint Expression of cellular prion protein on platelets from patients with gray platelet or Hermansky-Pudlak syndrome and the protein's association with alpha-granules
    Karel Holada
    Institute of Immunology and Microbiology, 1st Faculty of Medicine Charles University Prague, Studnickova 7, 128 20 Prague 2, Czech Republic
    Haematologica 91:1126-9. 2006
    ..Our results warrant further studies on the role of platelet PrPc in the transmission of prion diseases by blood transfusion...
  2. ncbi request reprint Divergent expression of cellular prion protein on blood cells of human and nonhuman primates
    Karel Holada
    Department of Immunology and Microbiology, 1st Medical Faculty, Charles University, Prague, Czech Republic
    Transfusion 47:2223-32. 2007
    ..Nonhuman primates are the most relevant models of human prion diseases...
  3. doi request reprint Underestimation of the expression of cellular prion protein on human red blood cells
    Martin Panigaj
    Institute of Immunology and Microbiology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic
    Transfusion 51:1012-21. 2011
    ..According to published studies, most of PrP(C) in blood cells resides in platelets (PLTs) and white blood cells...
  4. doi request reprint Blood storage affects the detection of cellular prion protein on peripheral blood leukocytes and circulating dendritic cells in part by promoting platelet satellitism
    Hana Glier
    Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
    J Immunol Methods 380:65-72. 2012
    ..However, flow cytometry data usually show large individual variations in detected PrP(C) levels in both infected and control groups, rendering the interpretation of individual patient data difficult...
  5. pmc Development of monoclonal antibodies specific for glycated prion protein
    Eva Dvorakova
    Institute of Immunology and Microbiology, 1st Faculty of Medicine, Charles University Prague, Studnickova 7, Prague 2, Czech Republic
    J Toxicol Environ Health A 74:1469-75. 2011
    ..Besides possible implication in TSE diagnostics, the antibodies may serve as tolls to advance our knowledge regarding the role of glycation in the prion pathophysiology...
  6. pmc Expression of prion protein in mouse erythroid progenitors and differentiating murine erythroleukemia cells
    Martin Panigaj
    Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
    PLoS ONE 6:e24599. 2011
    ..The efficient silencing of PrP(C) levels, despite not affecting MEL cell differentiation, enables created MEL lines to be used for studies of PrP(C) cellular function...
  7. doi request reprint Cellular prion protein in blood platelets associates with both lipid rafts and the cytoskeleton
    Adela Brouckova
    Charles University, Institute of Immunology and Microbiology, Prague 2, Czech Republic
    Thromb Haemost 102:966-74. 2009
    ..This localisation places PrPc in a position where it can interact with proteins involved in platelet signalling and eventually with vCJD prions...
  8. doi request reprint Photodynamic inactivation of prions by disulfonated hydroxyaluminium phthalocyanine
    Olga Janouskova
    Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague, Studnickova 7, 128 00 Prague 2, Czech Republic
    J Gen Virol 93:2512-7. 2012
    ..The photodynamic potential of Pcs offers a new way to inactivate prions using biodegradable compounds at room temperature and normal pressure, which could be useful for treating thermolabile materials and liquids...
  9. ncbi request reprint Reduced erythroid cell and erythropoietin production in response to acute anemia in prion protein-deficient (Prnp-/-) mice
    Jan H Zivny
    1st School of Medicine, Charles University, Prague, Czech Republic
    Blood Cells Mol Dis 40:302-7. 2008
    ..These observations indicate that PrPc may modulate tissue hypoxia-sensing mechanisms or effect hypoxia target gene expression...
  10. doi request reprint Gerstmann-Sträussler-Scheinker syndrome with the P102L pathogenic mutation presenting as familial Creutzfeldt-Jakob disease: a case report and review of the literature
    Robert Rusina
    Department of Neurology, Thomayer Teaching Hospital and Institute for Postgraduate Education in Medicine, Prague, Czech Republic
    Neurocase 19:41-53. 2013
    ....
  11. ncbi request reprint Activated platelets of patients with paroxysmal nocturnal hemoglobinuria express cellular prion protein
    Karel Holada
    Laboratory of Cellular Hematology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    Blood 100:341-3. 2002
    ..Our results indicate that in the absence of GPI anchor, PrPc can be expressed intracellularly and up-regulated on the platelet membrane, likely in a transmembrane form with the C-terminal part of the molecule inserted into the cytoplasm...
  12. ncbi request reprint Cellular prion protein is expressed on endothelial cells and is released during apoptosis on membrane microparticles found in human plasma
    Jan Simak
    Laboratory of Cellular Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 28092, USA
    Transfusion 42:334-42. 2002
    ..In orally infected animals, PrPsc accumulates in intestinal endothelial cells. In blood, two thirds of PrPc resides in plasma, but its source is not known...
  13. ncbi request reprint Elevated circulating endothelial membrane microparticles in paroxysmal nocturnal haemoglobinuria
    Jan Simak
    Laboratory of Cellular Hematology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD, USA
    Br J Haematol 125:804-13. 2004
    ..Analysis of circulating ECMP appears promising to provide useful information on the status of the vascular endothelium in PNH and SCD...
  14. ncbi request reprint CD34+ cells from paroxysmal nocturnal hemoglobinuria (PNH) patients are deficient in surface expression of cellular prion protein (PrPc)
    Antonio M Risitano
    Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Exp Hematol 31:65-72. 2003
    ..Conversely, PrP(c) is a good model to elucidate the fate of GPI-AP in PIG-A-deficient cells...
  15. pmc Scrapie infectivity in hamster blood is not associated with platelets
    Karel Holada
    Laboratory of Cellular Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
    J Virol 76:4649-50. 2002
    ..This result indicates that platelets are not the source of blood-borne infectivity in transmissible spongiform encephalopathy-infected hamsters...