Jaroslav Chladek

Summary

Affiliation: Charles University
Country: Czech Republic

Publications

  1. doi request reprint Assessment of methotrexate hepatotoxicity in psoriasis patients: a prospective evaluation of four serum fibrosis markers
    J Chladek
    Department of Pharmacology, Faculty of Medicine, Charles University, Hradec Kralove, Czech Republic
    J Eur Acad Dermatol Venereol 27:1007-14. 2013
  2. ncbi request reprint High-performance liquid chromatographic assay for the determination of 5-methyltetrahydrofolate in human plasma
    J Chladek
    Department of Pharmacology, Faculty of Medicine, Charles University, Hradec Kralove, Czech Republic
    J Chromatogr B Biomed Sci Appl 744:307-13. 2000
  3. ncbi request reprint No effect of phenobarbital pretreatment of rats on methotrexate pharmacokinetics in the isolated liver perfused in a single-pass way
    J Chladek
    Department of Pharmacology, Faculty of Medicine, Charles University, Hradec Kralove, Czech Republic
    Pharmacology 62:92-7. 2001
  4. ncbi request reprint Bioequivalence of two rimantadine tablet formulations in healthy male volunteers after single dose administration
    J Chladek
    Department of Pharmacology, Faculty of Medicine, Charles University, Hradec Kralove, Czech Republic
    Int J Clin Pharmacol Ther 39:179-84. 2001
  5. ncbi request reprint The effect of folic acid supplementation on the pharmacokinetics and pharmacodynamics of oral methotrexate during the remission-induction period of treatment for moderate-to-severe plaque psoriasis
    Jaroslav Chladek
    Department of Pharmacology, Faculty of Medicine, Charles University, Simkova 870, 500 38, Hradec Kralove, Czech Republic
    Eur J Clin Pharmacol 64:347-55. 2008
  6. ncbi request reprint Low-dose methotrexate pharmacokinetics and pharmacodynamics in the therapy of severe psoriasis
    Jaroslav Chladek
    Department of Pharmacology, Charles University, Prague, Czech Republic
    Basic Clin Pharmacol Toxicol 96:247-8. 2005
  7. ncbi request reprint Intravenous application of HI-6 salts (dichloride and dimethansulphonate) in pigs: comparison with pharmacokinetics profile after intramuscular administration
    Jana Zdarova Karasova
    Biomedical Research Center, University Hospital, Hradec Kralove, Czech Republic
    Neuro Endocrinol Lett 34:74-8. 2013
  8. doi request reprint Alveolar concentration and bronchial flux of nitric oxide: two linear modeling methods evaluated in children and adolescents with allergic rhinitis and atopic asthma
    Jirina Chladkova
    Department of Pediatrics, Charles University in Prague, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic
    Pediatr Pulmonol 47:1070-9. 2012
  9. doi request reprint Hyaluronidase: its effects on HI-6 dichloride and dimethanesulphonate pharmacokinetic profile in pigs
    Jana Zdarova Karasova
    Biomedical Research Center, University Hospital, Hradec Kralove, Czech Republic
    Toxicol Lett 220:167-71. 2013
  10. doi request reprint 677TT genotype is associated with elevated risk of methotrexate (MTX) toxicity in juvenile idiopathic arthritis: treatment outcome, erythrocyte concentrations of MTX and folates, and MTHFR polymorphisms
    Jana Tuková
    Department of Paediatrics and Adolescent Medicine, Charles University in Prague, 1st Medical School, Prague, Czech Republic
    J Rheumatol 37:2180-6. 2010

Collaborators

Detail Information

Publications26

  1. doi request reprint Assessment of methotrexate hepatotoxicity in psoriasis patients: a prospective evaluation of four serum fibrosis markers
    J Chladek
    Department of Pharmacology, Faculty of Medicine, Charles University, Hradec Kralove, Czech Republic
    J Eur Acad Dermatol Venereol 27:1007-14. 2013
    ..Low-dose oral methotrexate (MTX) is an effective immunosuppressive therapy for chronic plaque psoriasis. However, its use is hampered by the risk of liver fibrosis...
  2. ncbi request reprint High-performance liquid chromatographic assay for the determination of 5-methyltetrahydrofolate in human plasma
    J Chladek
    Department of Pharmacology, Faculty of Medicine, Charles University, Hradec Kralove, Czech Republic
    J Chromatogr B Biomed Sci Appl 744:307-13. 2000
    ..The limit of detection was 0.17 pmol which corresponds to a plasma concentration of 1.7 nmol/l. Thus, the assay could potentially be used for the measurement of 5-MTHF in the range of physiological concentrations in plasma (5-20 nmol/l)...
  3. ncbi request reprint No effect of phenobarbital pretreatment of rats on methotrexate pharmacokinetics in the isolated liver perfused in a single-pass way
    J Chladek
    Department of Pharmacology, Faculty of Medicine, Charles University, Hradec Kralove, Czech Republic
    Pharmacology 62:92-7. 2001
    ..The results suggest that in rats, pretreatment with PB has no effect on the hepatobiliary transport and hydroxylation of MTX...
  4. ncbi request reprint Bioequivalence of two rimantadine tablet formulations in healthy male volunteers after single dose administration
    J Chladek
    Department of Pharmacology, Faculty of Medicine, Charles University, Hradec Kralove, Czech Republic
    Int J Clin Pharmacol Ther 39:179-84. 2001
    ..The bioequivalence of two rimantadine tablet formulations was determined...
  5. ncbi request reprint The effect of folic acid supplementation on the pharmacokinetics and pharmacodynamics of oral methotrexate during the remission-induction period of treatment for moderate-to-severe plaque psoriasis
    Jaroslav Chladek
    Department of Pharmacology, Faculty of Medicine, Charles University, Simkova 870, 500 38, Hradec Kralove, Czech Republic
    Eur J Clin Pharmacol 64:347-55. 2008
    ..We assessed the effect of folic acid (FA) on the pharmacokinetics and pharmacodynamics of low-dose oral methotrexate (MTX) during the remission-induction phase of psoriasis treatment...
  6. ncbi request reprint Low-dose methotrexate pharmacokinetics and pharmacodynamics in the therapy of severe psoriasis
    Jaroslav Chladek
    Department of Pharmacology, Charles University, Prague, Czech Republic
    Basic Clin Pharmacol Toxicol 96:247-8. 2005
  7. ncbi request reprint Intravenous application of HI-6 salts (dichloride and dimethansulphonate) in pigs: comparison with pharmacokinetics profile after intramuscular administration
    Jana Zdarova Karasova
    Biomedical Research Center, University Hospital, Hradec Kralove, Czech Republic
    Neuro Endocrinol Lett 34:74-8. 2013
    ..We evaluated the pharmacokinetic profiles of two HI-6 salts after their intravenous (i.v.) administration, and compare the results with the known pharmacokinetics after i.m. administration...
  8. doi request reprint Alveolar concentration and bronchial flux of nitric oxide: two linear modeling methods evaluated in children and adolescents with allergic rhinitis and atopic asthma
    Jirina Chladkova
    Department of Pediatrics, Charles University in Prague, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic
    Pediatr Pulmonol 47:1070-9. 2012
    ..Furthermore, differences between patients with atopic asthma (AA) and allergic rhinitis (AR) and between methods P and T were assessed...
  9. doi request reprint Hyaluronidase: its effects on HI-6 dichloride and dimethanesulphonate pharmacokinetic profile in pigs
    Jana Zdarova Karasova
    Biomedical Research Center, University Hospital, Hradec Kralove, Czech Republic
    Toxicol Lett 220:167-71. 2013
    ..Improved bioavailability could to go hand in hand with increased effectiveness of therapy without the need of multiple autoinjector applications...
  10. doi request reprint 677TT genotype is associated with elevated risk of methotrexate (MTX) toxicity in juvenile idiopathic arthritis: treatment outcome, erythrocyte concentrations of MTX and folates, and MTHFR polymorphisms
    Jana Tuková
    Department of Paediatrics and Adolescent Medicine, Charles University in Prague, 1st Medical School, Prague, Czech Republic
    J Rheumatol 37:2180-6. 2010
    ....
  11. doi request reprint An improved high-performance liquid chromatography method for quantification of methotrexate polyglutamates in red blood cells of children with juvenile idiopathic arthritis
    Milos Hroch
    Department of Pharmacology, Charles University in Prague, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic
    Biopharm Drug Dispos 30:138-48. 2009
    ..9 and 32.9 nmol/l, respectively. The method was proved suitable for monitoring the concentration of methotrexate polyglutamates in erythrocytes of patients with juvenile idiopathic arthritis...
  12. doi request reprint Activation of p38 MAPK and expression of TGF-β1 in rat colon enterocytes after whole body γ-irradiation
    Jaroslav Pejchal
    Center of Advanced Studies, University of Defence, Hradec Kralove, Czech Republic
    Int J Radiat Biol 88:348-58. 2012
    ....
  13. ncbi request reprint Pharmacokinetics and pharmacodynamics of methotrexate in non-neoplastic diseases
    Jiri Grim
    Department of Pharmacology, Charles University, Hradec Kralove, Czech Republic
    Clin Pharmacokinet 42:139-51. 2003
    ..However, the data are still in the process of being completed and are not yet published...
  14. doi request reprint High-dose methotrexate in children with acute lymphoblastic leukemia: 7-hydroxymethotrexate systemic exposure and urinary concentrations at the steady state correlate well with those of methotrexate
    Jirina Chladkova
    Department of Paediatrics, Charles University in Prague, Faculty of Medicine and Hospital, Hradec Kralove, Czech Republic
    Arzneimittelforschung 60:769-75. 2010
    ....
  15. ncbi request reprint Lithium microdialysis and its use for monitoring of stomach and colon submucosal blood perfusion--a pilot study using ischemic preconditioning in rats
    Norbert Cibicek
    Charles University in Prague, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic
    Acta Medica (Hradec Kralove) 49:227-31. 2006
    ..However, we failed to demonstrate any beneficial effect of ISP on pancreas, systemic nitric oxide and local/remote microcirculation within studied organs...
  16. ncbi request reprint A rat model of early sepsis: relationships between gentamicin pharmacokinetics and systemic and renal effects of bacterial lipopolysaccharide combined with interleukin-2
    Sarka Studena
    Department of Pharmacology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Czech Republic
    Biol Pharm Bull 35:1703-10. 2012
    ..Nonrenal routes which, for the most part, compensate the reduced renal CL(ge) in septic rats deserve further study...
  17. doi request reprint HPLC determination of arginases inhibitor N-(ω)-hydroxy-nor-L-arginine using core-shell particle column and LC-MS/MS identification of principal metabolite in rat plasma
    Milos Hroch
    Charles University in Prague, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic
    J Chromatogr B Analyt Technol Biomed Life Sci 880:90-9. 2012
    ..v. 10 mg kg(-1)). The metabolite was identified as nor-L-arginine using mass spectrometry. Validated method was successfully used for pilot pharmacokinetic experiment on rats...
  18. doi request reprint Analysis of single-breath profiles of exhaled nitric oxide in children with allergy and asthma: guideline-derived plateau concentrations compared to results of automatic evaluation by two analyzers
    Jirina Chladkova
    Department of Pediatrics, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Hradec Kralove, Czech Republic
    J Asthma 45:820-6. 2008
    ..The Ecomedics FE(NO) concentrations of children more than 12 years of age calculated in the interval of 7 to 10 seconds represent plateau values and agree well with NIOX MINO results obtained during a standard 10-second exhalation...
  19. ncbi request reprint Validation of nitrite and nitrate measurements in exhaled breath condensate
    Jirina Chladkova
    Department of Pediatrics, Charles University in Prague, Faculty of Medicine in Hradec Kralove, Prague, Czech Republic
    Respiration 73:173-9. 2006
    ..EBC concentrations of nitrite and nitrate, the stable end products of oxidative metabolism of nitric oxide, are increased in patients with asthma, especially during acute exacerbations...
  20. pmc Pharmacokinetics and pharmacodynamics of low-dose methotrexate in the treatment of psoriasis
    Jaroslav Chladek
    Department of Pharmacology, Faculty of Medicine Charles University, Hradec Kralove, Czech Republic
    Br J Clin Pharmacol 54:147-56. 2002
    ..The aim of this 13 week, randomized, parallel-group study was to evaluate the relationship between the pharmacokinetics (PK) and pharmacodynamics (PD) of low-dose intermittent oral methotrexate (LDMTX) in patients with psoriasis...
  21. ncbi request reprint Single- and multiple-dose pharmacokinetics of arginase inhibitor Nω-hydroxy-nor-L-arginine, and its effect on plasma amino acids concentrations in Wistar rats
    Zuzana Havlínová
    Department of Pharmacology, Faculty of Medicine, Charles University, Hradec Kralove, Czech Republic
    Gen Physiol Biophys 33:189-98. 2014
    ..In conclusion, pharmacokinetic data of the present study can guide rational dosing of nor-NOHA in future studies. Limitations of the strategy of NO modulation via arginase inhibition should be further explored. ..
  22. doi request reprint Early changes in L-arginine-nitric oxide metabolic pathways in response to the whole-body gamma irradiation of rats
    Andrea Babicova
    Department of Medical Biochemistry, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic
    Int J Radiat Biol 87:1067-73. 2011
    ..This study investigated the changes in L-arginine-NO metabolic pathways within a 24-hour period after whole-body gamma irradiation of rats with the range of low to supra-lethal doses...
  23. doi request reprint Tolerability and outcomes of kinetically guided therapy with gentamicin in critically ill neonates during the first week of life: an open-label, prospective study
    Jirina Martinkova
    Department of Pharmacology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Hradec Kralove, Czech Republic
    Clin Ther 32:2400-14. 2010
    ..Critically ill neonates constitute a unique challenge in dosing owing to the pathologic alterations that accompany severe illness and the rapidly changing conditions of these patients...
  24. doi request reprint Pharmacokinetic study of two acetylcholinesterase reactivators, trimedoxime and newly synthesized oxime K027, in rat plasma
    Jana Zdarova Karasova
    Department of Public Health, Faculty of Health Sciences, Defence University, Hradec Kralove, Czech Republic
    J Appl Toxicol 33:18-23. 2013
    ..In conclusion, oxime K027 might have superior pK properties that may be translated in its faster absorption and subsequent tissue distribution...
  25. ncbi request reprint Inhibitory effects of memantine on human cytochrome P450 activities: prediction of in vivo drug interactions
    Stanislav Micuda
    Department of Pharmacology, Medical Faculty of Charles University, Simkova 870, PO Box 38, 500 38, Hradec Kralove, Czech Republic
    Eur J Clin Pharmacol 60:583-9. 2004
    ..The aim of the present study was to predict the drug interaction potential of memantine by elucidation of its inhibitory effects on cytochrome P450 enzymes using pooled human liver microsomes (HLM) and recombinant P450s...
  26. ncbi request reprint Pharmacokinetics and tissue distribution of platinum in rats following single and multiple oral doses of LA-12 [(OC-6-43)-bis(acetato)(1-adamantylamine)amminedichloroplatinum(IV)]
    Petr Sova
    PLIVA Lachema a s, Brno, Czech Republic
    Int J Pharm 288:123-9. 2005
    ..3 to 38.6 mg kg(-1). At the same time, the increase in total plasma Pt concentrations was 40% less than proportional. Concentrations of Pt in the plasma ultrafiltrate decreased rapidly with the initial half-life of 1 h...