Research Topics
| H SychrovaSummaryAffiliation: Academy of Sciences of the Czech Republic Country: Czech Republic Publications
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Detail Information
Publications
Involvement of Nha1 antiporter in regulation of intracellular pH in Saccharomyces cerevisiaeH Sychrova
Department of Membrane Transport, Czech Academy of Sciences, Prague, Czech Republic
FEMS Microbiol Lett 171:167-72. 1999..The H+/K+(Na+) exchange mechanism of Nha1p was confirmed in reconstituted plasma membrane vesicles...
Organization of specific genomic regions of Zygosaccharomyces rouxii and Pichia sorbitophila: comparison with Saccharomyces cerevisiaeH Sychrova
Department of Membrane Transport, Institute of Physiology CzAcadSci, Videnska 1083, 142 20 Prague 4, Czech Republic
Yeast 16:1377-85. 2000..cerevisiae. According to our results, the presence of two overlapping ORFs in S. cerevisiae (YJL107c-YJL108c) could be interpreted as the result of a frameshift mutation...
Functional comparison of plasma-membrane Na+/H+ antiporters from two pathogenic Candida speciesYannick Krauke
Department of Membrane Transport, Institute of Physiology AS CR, V, V, I, Videnska 1083, 14220 Prague 4, Czech Republic
BMC Microbiol 8:80. 2008....
Yeast as a model organism to study transport and homeostasis of alkali metal cationsH Sychrova
Department of Membrane Transport, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
Physiol Res 53:S91-8. 2004....
Molecular cloning and sequence analysis of the Zygosaccharomyces rouxiiLEU2 gene encoding a beta-isopropylmalate dehydrogenaseH Sychrova
Department of Membrane Transport, Institute of Physiology, CzAcadSci, 142 20 Prague 4, Czech Republic
Yeast 18:989-94. 2001..The cloned DNA fragment fully complemented the leu2 mutations of Saccharomyces cerevisiae and Z. rouxii...
Molecular cloning and sequence analysis of Zygosaccharomyces rouxii ADE2 gene encoding a phosphoribosyl-aminoimidazole carboxylaseH Sychrova
Department of Membrane Transport, Institute of Physiology CzAcadSci, 142 20 Prague 4, Czech Republic
Yeast 15:1399-402. 1999..The sequenced DNA fragment contains a 1710 bp open reading frame predicting a protein of 570 amino acids. The deduced amino acid sequence shares a high degree of homology with Ade2p homologues in five other yeast species...
Sequence and organization analyses of a Zygosaccharomyces rouxii DNA fragment containing the HIS3 geneH Sychrova
Department of Membrane Transport, Institute of Physiology CzAcadSci, 142 20 Prague 4, Czech Republic
Yeast 16:581-7. 2000..In the promoter region of the putative ZrHIS3 gene, a T(c) element required for constitutive transcription was found. The GenBank Accession No. of the sequenced DNA region is Y18561...
Functional study of the Saccharomyces cerevisiae Nha1p C-terminusO Kinclová
Department of Membrane Transport, Institute of Physiology CzAcadSci, 14220 Prague 4, Czech Republic
Mol Microbiol 40:656-68. 2001..We also present evidence that the Nha1p C-terminus is involved in the cell response to sudden changes in environmental osmolarity...
Exploration of yeast alkali metal cation/H+ antiporters: sequence and structure comparisonL Pribylova
Department of Membrane Transport, Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia
Folia Microbiol (Praha) 51:413-24. 2006..Yeast Kha1 proteins probably belong to the same subfamily as bacterial antiporters, whereas Nhal proteins form a distinct subfamily...
The Candida albicans Na(+)/H(+) antiporter exports potassium and rubidiumO Kinclová
Department of Membrane Transport, Institute of Physiology, Prague, Czech Republic
FEBS Lett 504:11-5. 2001..The cation efflux activity of Cnh1p in S. cerevisiae depends on the gradient of protons across the plasma membrane, and a Cnh1p-mediated K(+) efflux is involved in a cell response to sudden rise of cytoplasmic pH...
Physiological characterization of osmotolerant yeast Pichia sorbitophila and comparison with a putative synonym Pichia farinosaL Maresova
Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia
Folia Microbiol (Praha) 48:211-7. 2003..farinosa, comparative physiological studies together with PCR amplification of P. farinosa DNA fragments homologous to known P. sorbitophila genes provided a strong indication that this strain should be classified as a separate species...
Trk2 transporter is a relevant player in K+ supply and plasma-membrane potential control in Saccharomyces cerevisiaeS Petrezselyova
Department of Membrane Transport, Institute of Physiology, Academy of Sciences of the Czech Republic, v v i, 14220, Prague 4, Czech Republic
Folia Microbiol (Praha) 56:23-8. 2011....
Rice Na+/H+-antiporter Nhx1 partially complements the alkali-metal-cation sensitivity of yeast strains lacking three sodium transportersO Kinclova-Zimmermannova
Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czechia
Folia Microbiol (Praha) 49:519-25. 2004..Its activity contributes to sequestration of alkali metal cations in intracellular vesicles...
Production of Yarrowia lipolytica Nha2 Na+/H+ antiporter improves the salt tolerance of Saccharomyces cerevisiaeK Papouskova
Department of Membrane Transport, Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia
Folia Microbiol (Praha) 52:600-2. 2007..Its heterologous expression in Saccharomyces cerevisiae wild-type laboratory strains increased their sodium tolerance more efficiently than the expression of ZrSod2-22 antiporter from the osmotolerant yeast Zygosaccharomvces rouxii...
The Zygosaccharomyces rouxii strain CBS732 contains only one copy of the HOG1 and the SOD2 genesO Kinclová
Department of Membrane Transport, Institute of Physiology CzAcadSci, Videnska 1083, 142 20 4, Prague, Czech Republic
J Biotechnol 88:151-8. 2001..cerevisiae shows better activity toward toxic Na(+) and Li(+) cations than does S. cerevisiae's own Nha1 antiporter, and is efficient in improving the halotolerance of some S. cerevisiae wild types...
Chimeras between C. glabrata Cnh1 and S. cerevisiae Nha1 Na+/H+-antiporters are functional proteins increasing the salt tolerance of yeast cellsY Krauke
Department of Membrane Transport, Institute of Physiology Academy of Sciences of the Czech Republic v v i, 142 20 Prague, Czech Republic
Folia Microbiol (Praha) 55:435-41. 2010..cerevisiae and C. glabrata plasma-membrane Na(+)/H(+) antiporters, various parts of these proteins are not exchangeable without affecting the antiporter's transport capacity...
Differences in osmotolerant and cell-wall properties of two Zygosaccharomyces rouxii strainsL Pribylova
Department of Membrane Transport, Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia
Folia Microbiol (Praha) 52:241-5. 2007....
Expression of the Saccharomyces cerevisiae MPR1 gene encoding N-acetyltransferase in Zygosaccharomyces rouxii confers resistance to L-azetidine-2-carboxylateL Pribylova
Department of Membrane Transport, Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia
Folia Microbiol (Praha) 51:203-7. 2006..The same was found for an AZC-sensitive S. cerevisiae strain in which the ScMPR1 was expressed. In both yeasts, ScMPR1 can be used only as an auxiliary marker gene...
The Candida albicans GAP gene family encodes permeases involved in general and specific amino acid uptake and sensingLucie Kraidlova
Department of Membrane Transport, Institute of Physiology AS CR, Videnska 1083, 142 20 Prague 4, Czech Republic
Eukaryot Cell 10:1219-29. 2011..Our data show that CaGAP genes can be functionally expressed in S. cerevisiae and that CaGap permeases communicate to the intracellular signal transduction pathway similarly to ScGap1...
