P Svoboda

Summary

Affiliation: Academy of Sciences of the Czech Republic
Country: Czech Republic

Publications

  1. ncbi request reprint Opioid-receptor (OR) signaling cascades in rat cerebral cortex and model cell lines: the role of plasma membrane structure
    H Ujcikova
    Department of Biochemistry of Membrane Receptors, Institute of Physiology Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Physiol Res 63:S165-76. 2014
  2. ncbi request reprint Plasma membrane density of GABA(B)-R1a, GABA(B)-R1b, GABA-R2 and trimeric G-proteins in the course of postnatal development of rat brain cortex
    K Dlouha
    Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Physiol Res 62:547-59. 2013
  3. ncbi request reprint Hormone-induced subcellular redistribution of trimeric G proteins
    P Svoboda
    Institute of Physiology, Academy of Sciences of the Czech Republic, Prague
    Cell Mol Life Sci 59:501-12. 2002
  4. ncbi request reprint Biochemistry of transmembrane signaling mediated by trimeric G proteins
    P Svoboda
    Department of Membrane Receptors, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Physiol Res 53:S141-52. 2004
  5. ncbi request reprint Subcellular redistribution of trimeric G-proteins--potential mechanism of desensitization of hormone response: internalization, solubilization, down-regulation
    Z Drastichova
    Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic
    Physiol Res 57:S1-10. 2008
  6. ncbi request reprint Membrane-Bound and cytosolic forms of heterotrimeric G proteins in young and adult rat myocardium: influence of neonatal hypo- and hyperthyroidism
    J Novotny
    Institute of Physiology, Academy of Sciences of the Czech Republic, Prague
    J Cell Biochem 82:215-24. 2001
  7. ncbi request reprint Ontogenetic development of GABA(B)-receptor signaling cascade in plasma membranes isolated from rat brain cortex; the number of GABA(B)-receptors is high already shortly after the birth
    D Kagan
    Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Physiol Res 61:629-35. 2012
  8. ncbi request reprint The time-course of agonist-induced solubilization of trimeric G(q)alpha/G(11)alpha proteins resolved by two-dimensional electrophoresis
    D Durchankova
    Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Physiol Res 57:195-203. 2008
  9. ncbi request reprint Subcellular shifts of trimeric G-proteins following activation of baker's yeast by glucose
    A Kotyk
    Department of Membrane Transport, Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia
    Folia Microbiol (Praha) 46:391-6. 2001
  10. ncbi request reprint Biguanides inhibit complex I, II and IV of rat liver mitochondria and modify their functional properties
    Z Drahota
    Centre of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
    Physiol Res 63:1-11. 2014

Collaborators

  • V Lisy
  • M Cahova
  • A Kotyk
  • P Mares
  • T Soukup
  • G Milligan
  • K Dlouha
  • D Kagan
  • M Vosahlikova
  • J Novotny
  • H Ujcikova
  • L Roubalová
  • Z Drastichova
  • D Durchankova
  • J Brejchová
  • Z Drahota
  • P Ostasov
  • F W Khwaja
  • M Hof
  • M Parenti
  • M Kalous
  • E Páleníčková
  • M Milerova
  • J Sykora
  • L Kazdova
  • L Merta
  • Z Cervinkova
  • R Endlicher
  • I Ihnatovych
  • J Stohr
  • L Hejnova
  • L Bourova
  • J Teisinger
  • V Rudajev
  • J Pohl
  • B Pyrzynska
  • E G Van Meir
  • M Reed

Detail Information

Publications14

  1. ncbi request reprint Opioid-receptor (OR) signaling cascades in rat cerebral cortex and model cell lines: the role of plasma membrane structure
    H Ujcikova
    Department of Biochemistry of Membrane Receptors, Institute of Physiology Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Physiol Res 63:S165-76. 2014
    ....
  2. ncbi request reprint Plasma membrane density of GABA(B)-R1a, GABA(B)-R1b, GABA-R2 and trimeric G-proteins in the course of postnatal development of rat brain cortex
    K Dlouha
    Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Physiol Res 62:547-59. 2013
    ..Major change occurred between the birth and PD25. In adult rats, membrane content of Na, K-ATPase was 3-times higher than around the birth. ..
  3. ncbi request reprint Hormone-induced subcellular redistribution of trimeric G proteins
    P Svoboda
    Institute of Physiology, Academy of Sciences of the Czech Republic, Prague
    Cell Mol Life Sci 59:501-12. 2002
    ..e., beside receptor-based mechanisms) to dampen the hormone or neurotransmitter response after sustained (long-term) exposure...
  4. ncbi request reprint Biochemistry of transmembrane signaling mediated by trimeric G proteins
    P Svoboda
    Department of Membrane Receptors, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Physiol Res 53:S141-52. 2004
    ..This review provides general information about the basic principles of G protein-regulated transmembrane signaling as well as about our contribution to the current progress in the field...
  5. ncbi request reprint Subcellular redistribution of trimeric G-proteins--potential mechanism of desensitization of hormone response: internalization, solubilization, down-regulation
    Z Drastichova
    Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic
    Physiol Res 57:S1-10. 2008
    ..We have also mentioned sodium plus potassium dependent adenosine triphosphatase (Na, K-ATPase) and 3H-ouabain binding as useful reference standard of plasma membrane purity in the brain...
  6. ncbi request reprint Membrane-Bound and cytosolic forms of heterotrimeric G proteins in young and adult rat myocardium: influence of neonatal hypo- and hyperthyroidism
    J Novotny
    Institute of Physiology, Academy of Sciences of the Czech Republic, Prague
    J Cell Biochem 82:215-24. 2001
    ..Alterations in the membrane-cytosol balance of G protein subunits associated with maturation or induced by altered thyroid status indicate physiological importance of cytosolic forms of these proteins in the rat myocardium...
  7. ncbi request reprint Ontogenetic development of GABA(B)-receptor signaling cascade in plasma membranes isolated from rat brain cortex; the number of GABA(B)-receptors is high already shortly after the birth
    D Kagan
    Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Physiol Res 61:629-35. 2012
    ..The further development was reflected in a decrease of [(3)H]CGP54626 binding as the B(max) values of 1.38+/-0.05 and 0.93+/-0.04 pmol · mg(-1) were determined in PM isolated from 13- and 90-days old rats, respectively...
  8. ncbi request reprint The time-course of agonist-induced solubilization of trimeric G(q)alpha/G(11)alpha proteins resolved by two-dimensional electrophoresis
    D Durchankova
    Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Physiol Res 57:195-203. 2008
    ..Our data therefore rather indicate tight/persisting binding of G(q)alpha/G(11)alpha proteins to the membrane...
  9. ncbi request reprint Subcellular shifts of trimeric G-proteins following activation of baker's yeast by glucose
    A Kotyk
    Department of Membrane Transport, Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia
    Folia Microbiol (Praha) 46:391-6. 2001
    ..The question remains open as to the functional significance of the internalized forms of the G-proteins as these remain sequestered from the plasma membrane well after glucose has been consumed...
  10. ncbi request reprint Biguanides inhibit complex I, II and IV of rat liver mitochondria and modify their functional properties
    Z Drahota
    Centre of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
    Physiol Res 63:1-11. 2014
    ..Our data therefore suggest that biguanides could be considered as agents with high efficacy but low specifity. ..
  11. ncbi request reprint Maturation of rat brain is accompanied by differential expression of the long and short splice variants of G(s)alpha protein: identification of cytosolic forms of G(s)alpha
    I Ihnatovych
    Department of Developmental Epileptology and Biochemistry of Membrane Receptors, Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska, Prague, Czech Republic
    J Neurochem 79:88-97. 2001
    ..The number of beta-adrenoceptors increased sharply during early post-natal development but only slightly in adulthood, and both GTP- and isoproterenol-stimulated adenylate cyclase activity reached peak values around PD12...
  12. ncbi request reprint The influence of monovalent cations on trimeric G protein G(i)1α activity in HEK293 cells stably expressing DOR-G(i)1α (Cys(351)-Ile(351)) fusion protein
    M Vosahlikova
    Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Physiol Res 60:541-7. 2011
    ....
  13. ncbi request reprint Proteomic identification of the wt-p53-regulated tumor cell secretome
    F W Khwaja
    Laboratory of Molecular Neuro Oncology, Department of Neurosurgery, Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA 30322, USA
    Oncogene 25:7650-61. 2006
    ..Evidence for p53-controlled post-translational modifications on nine secreted proteins was also found. These findings will enhance our understanding of wt-p53 modulated interactions of the tumor with its environment...
  14. ncbi request reprint Agonist-induced transfer of the alpha subunits of the guanine-nucleotide-binding regulatory proteins Gq and G11 and of muscarinic m1 acetylcholine receptors from plasma membranes to a light-vesicular membrane fraction
    P Svoboda
    Department of Biochemistry, University of Glasgow, Scotland
    Eur J Biochem 224:455-62. 1994
    ..The second step is represented by an agonist-specific down-regulation pathway. Both the Hm1 receptor and Gq alpha/G11 alpha appear to follow similar sequestration and down-regulation patterns...