R J Sram

Summary

Affiliation: Academy of Sciences of the Czech Republic
Country: Czech Republic

Publications

  1. pmc Genetic, biochemical, and environmental factors associated with pregnancy outcomes in newborns from the Czech Republic
    Pavel Rossner
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Environ Health Perspect 119:265-71. 2011
  2. doi Health impact of air pollution to children
    Radim J Sram
    Institute of Experimental Medicine AS CR, Prague, Czech Republic
    Int J Hyg Environ Health 216:533-40. 2013
  3. doi Vitamin C for DNA damage prevention
    Radim J Sram
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, 14220 Prague 4, Czech Republic
    Mutat Res 733:39-49. 2012
  4. pmc Global gene expression changes in human embryonic lung fibroblasts induced by organic extracts from respirable air particles
    Helena Libalova
    Department of Genetic Ecotoxicology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic
    Part Fibre Toxicol 9:1. 2012
  5. pmc Air pollution exposure during critical time periods in gestation and alterations in cord blood lymphocyte distribution: a cohort of livebirths
    Caroline E W Herr
    Department of Public Health Sciences, University of California, Davis, USA
    Environ Health 9:46. 2010
  6. pmc Unfavourable birth outcomes of the Roma women in the Czech Republic and the potential explanations: a population-based study
    Martin Bobak
    International Centre for Health and Society, Department of Epidemiology and Public Health, University College London, 1 19 Torrington Place, London WC1E 6BT, UK
    BMC Public Health 5:106. 2005
  7. ncbi The impact of plasma folate levels of mothers and newborns on intrauterine growth retardation and birth weight
    Radim J Sram
    Institute of Experimental Medicine Academy of Sciences of Czech Republic, Health Institute of Central Bohemia, Videnska 1083, 14220 Prague 4, Czech Republic
    Mutat Res 591:302-10. 2005
  8. ncbi Chromosomal aberrations in environmentally exposed population in relation to metabolic and DNA repair genes polymorphisms
    Radim J Sram
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine AS CR and Health Institute of Central Bohemia, Videnska 1083, 142 20 Prague 4, Czech Republic
    Mutat Res 620:22-33. 2007
  9. ncbi Heat shock proteins hsp32 and hsp70 as biomarkers of an early response? In vitro induction of heat shock proteins after exposure of cell culture to carcinogenic compounds and their real mixtures
    Pavel Rossner
    Laboratory of Genetic Ecotoxicology, Health Institute of Central Bohemia and Institute of Experimental Medicine AS CR, Videnska 1083, 142 20 Prague 4, Czech Republic
    Mutat Res 542:105-16. 2003
  10. ncbi Possible genetic damage in the Czech nuclear power plant workers
    Radim J Sram
    Laboratory of Genetic Ecotoxicology, Health Institute of Central Bohemia and Institute of Experimental Medicine AS CR, Videnska 1083, 142 20 Praha 4, Czech Republic
    Mutat Res 593:50-63. 2006

Collaborators

Detail Information

Publications65

  1. pmc Genetic, biochemical, and environmental factors associated with pregnancy outcomes in newborns from the Czech Republic
    Pavel Rossner
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Environ Health Perspect 119:265-71. 2011
    ..Oxidative damage to placental DNA can result in negative pregnancy outcomes, including intrauterine growth restriction (IUGR) and low birth weight (LBW)...
  2. doi Health impact of air pollution to children
    Radim J Sram
    Institute of Experimental Medicine AS CR, Prague, Czech Republic
    Int J Hyg Environ Health 216:533-40. 2013
    ..Especially noticeable is the increase of respiratory morbidity. With the development of molecular epidemiology, we can further evaluate the health risk of air pollution using biomarkers. ..
  3. doi Vitamin C for DNA damage prevention
    Radim J Sram
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, 14220 Prague 4, Czech Republic
    Mutat Res 733:39-49. 2012
    ..It is possible that the impact of vitamin C on DNA damage depends both on background values of vitamin C in the individual as well as on the level of exposure to xenobiotics or oxidative stress...
  4. pmc Global gene expression changes in human embryonic lung fibroblasts induced by organic extracts from respirable air particles
    Helena Libalova
    Department of Genetic Ecotoxicology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic
    Part Fibre Toxicol 9:1. 2012
    ....
  5. pmc Air pollution exposure during critical time periods in gestation and alterations in cord blood lymphocyte distribution: a cohort of livebirths
    Caroline E W Herr
    Department of Public Health Sciences, University of California, Davis, USA
    Environ Health 9:46. 2010
    ..This study investigates the association between cord blood lymphocyte proportions and maternal exposure to air pollution during each gestational month...
  6. pmc Unfavourable birth outcomes of the Roma women in the Czech Republic and the potential explanations: a population-based study
    Martin Bobak
    International Centre for Health and Society, Department of Epidemiology and Public Health, University College London, 1 19 Torrington Place, London WC1E 6BT, UK
    BMC Public Health 5:106. 2005
    ..The objective of this study was to quantify the differences in birth outcomes between Roma and non-Roma mothers in the Czech Republic and to investigate the potential causes of such differences...
  7. ncbi The impact of plasma folate levels of mothers and newborns on intrauterine growth retardation and birth weight
    Radim J Sram
    Institute of Experimental Medicine Academy of Sciences of Czech Republic, Health Institute of Central Bohemia, Videnska 1083, 14220 Prague 4, Czech Republic
    Mutat Res 591:302-10. 2005
    ..It would be warranted to study the effect of folate levels on pregnancy outcomes in the relationship to different environmental pollution and life styles of mothers...
  8. ncbi Chromosomal aberrations in environmentally exposed population in relation to metabolic and DNA repair genes polymorphisms
    Radim J Sram
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine AS CR and Health Institute of Central Bohemia, Videnska 1083, 142 20 Prague 4, Czech Republic
    Mutat Res 620:22-33. 2007
    ..This is the first study that has reported a relationship between DNA adducts (biomarker of exposure) and chromosomal aberrations by FISH (biomarker of effect)...
  9. ncbi Heat shock proteins hsp32 and hsp70 as biomarkers of an early response? In vitro induction of heat shock proteins after exposure of cell culture to carcinogenic compounds and their real mixtures
    Pavel Rossner
    Laboratory of Genetic Ecotoxicology, Health Institute of Central Bohemia and Institute of Experimental Medicine AS CR, Videnska 1083, 142 20 Prague 4, Czech Republic
    Mutat Res 542:105-16. 2003
    ..We conclude that hsp 32 and hsp70 are not suitable biomarkers of an early exposure to PAHs, their nitro-derivates, aromatic amines or EOM under the conditions used...
  10. ncbi Possible genetic damage in the Czech nuclear power plant workers
    Radim J Sram
    Laboratory of Genetic Ecotoxicology, Health Institute of Central Bohemia and Institute of Experimental Medicine AS CR, Videnska 1083, 142 20 Praha 4, Czech Republic
    Mutat Res 593:50-63. 2006
    ..No association between the dose of irradiation and the level of chromosomal aberrations in any nuclear power plant was detected either by the conventional cytogenetic analysis or by FISH...
  11. ncbi Cytogenetic analysis and occupational health in the Czech Republic
    Radim J Sram
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, AS CR and Health Institute of the Central Bohemia, Vi denská 1083, 142 20 Prague 4, Czech Republic
    Mutat Res 566:21-48. 2004
    ....
  12. ncbi Cytogenetic analysis using fluorescence in situ hybridization (FISH) to evaluate occupational exposure to carcinogens
    Radim J Sram
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine AS CR and Health Institute of Central Bohemia, 142 20 Prague 4, Czech Republic
    Toxicol Lett 149:335-44. 2004
    ..72+/-1.57 versus 1.25+/-1.11, P<0.05). The method of FISH with whole chromosome painting seems to be more sensitive to detect chromosomal injury by occupational exposure to carcinogens than conventional method...
  13. pmc Future research directions to characterize environmental mutagens in highly polluted area
    R J Sram
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Environ Health Perspect 104:603-7. 1996
    ..Future research should include validating molecular methods, studying adaptive response to chemical carcinogens, and studying the modulatory effect of antioxidants, as well as the effect of carcinogens on immunity...
  14. pmc Molecular epidemiology studies on occupational and environmental exposure to mutagens and carcinogens, 1997-1999
    R J Sram
    Laboratory of Genetic Ecotoxicology, Regional Institute of Hygiene of Central Bohemia and Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Environ Health Perspect 108:57-70. 2000
    ..Until now there have not been sufficient data to interpret the relationship between genotypes, biomarkers of exposure, and biomarkers of effect for assessing the risk of human exposure to mutagens and carcinogens...
  15. ncbi Adverse reproductive outcomes from exposure to environmental mutagens
    R J Sram
    Laboratory of Genetic Ecotoxicology, Regional Institute of Hygiene of Central Bohemia, c o Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20, Prague, Czech Republic
    Mutat Res 428:203-15. 1999
    ..Pregnancy outcome and semen studies imply that relatively low air pollution (higher than 40 microg PM10/m(3)) can significantly increase the adverse reproductive outcomes affecting both genders...
  16. ncbi Environmental exposure to carcinogenic polycyclic aromatic hydrocarbons--the interpretation of cytogenetic analysis by FISH
    Radim J Sram
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine AS CR, v v i, and Health Institute of Central Bohemia, Videnska 1083, 142 20 Prague 4, Czech Republic
    Toxicol Lett 172:12-20. 2007
    ..This is the first study reporting that translocations induced by c-PAHs in peripheral lymphocytes last only several weeks...
  17. ncbi Chromosomal aberrations, sister-chromatid exchanges, cells with high frequency of SCE, micronuclei and comet assay parameters in 1, 3-butadiene-exposed workers
    R J Sram
    Laboratory of Genetic Ecotoxicology, c o Regional Institute of Hygiene of Central Bohemia and Institute of Experimental Medicine, Academy of Sciences of Czech Republic, Videnska 1083, 142 20, Prague, Czech Republic
    Mutat Res 419:145-54. 1998
    ..GSTM1 genotype affected chromosomal aberrations in exposed group, while GSTT1 genotype affected chromosomal aberrations in controls. No effect of GSTM1 or GSTT1 genotypes was observed on any other biomarkers used...
  18. ncbi Association of DNA adducts and genotypes with birth weight
    Radim J Sram
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, AS CR, Videnska 1083, 142 20 Prague, Czech Republic
    Mutat Res 608:121-8. 2006
    ..This means that in the future, the impact of air pollution on children's health should be studied together with their genetic polymorphisms...
  19. pmc Effect of glutathione S-transferase M1 polymorphisms on biomarkers of exposure and effects
    R J Sram
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine and Regional Institute of Hygiene of Central Bohemia, Prague, Czech Republic
    Environ Health Perspect 106:231-9. 1998
    ..The relationship between genotypes and biomarkers of exposure and effects may provide an important guide to the risk assessment of human exposure to mutagens and carcinogens...
  20. ncbi Single cell gel electrophoresis assay: sensitivity of peripheral white blood cells in human population studies
    R J Sram
    Institute of Experimental Medicine of the Academy of Science of the Czech Republic, Prague, Czech Republic
    Mutagenesis 13:99-103. 1998
    ..According to our results it seems that the Comet assay was not a particularly sensitive technique to determine the effects of environmental pollution at the DNA level if peripheral WBC are used...
  21. ncbi Chromosomal aberration frequencies determined by conventional methods: Parallel increases over time in the region of a petrochemical industry and throughout the Czech Republic
    Radim J Sram
    Institute of Experimental Medicine ASCR and Health Institute of Central Bohemia, Laboratory of Genetic Ecotoxicology, Videnska 1083, 142 20 Prague 4, Czech Republic
    Chem Biol Interact 166:239-44. 2007
    ..This substantial increase in %AB.C. values in 2000 and 2004, therefore, has occurred throughout the CR and is probably unrelated to chemicals uniquely present at the petrochemical industry site...
  22. pmc Ambient air pollution and pregnancy outcomes: a review of the literature
    Radim J Sram
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, Academy of Sciences, and Health Institute of Central Bohemia, Prague, Czech Republic
    Environ Health Perspect 113:375-82. 2005
    ....
  23. doi Biomarkers of exposure to tobacco smoke and environmental pollutants in mothers and their transplacental transfer to the foetus. Part II. Oxidative damage
    P Rossner
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, AS CR, Prague 4, Czech Republic
    Mutat Res 669:20-6. 2009
    ..Our results showed higher lipid peroxidation in newborns than in mothers, close correlation of analyzed oxidative stress markers between newborns and mothers, and a relationship between oxidative stress and induction of DNA adducts...
  24. ncbi Biomarkers of air pollution exposure--a study of policemen in Prague
    J Topinka
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic and the Health Institute of Central Bohemia, Videnska 1083, 142 20 Prague 4, Czech Republic
    Mutat Res 624:9-17. 2007
    ....
  25. doi Biomarkers of exposure to tobacco smoke and environmental pollutants in mothers and their transplacental transfer to the foetus. Part I: bulky DNA adducts
    J Topinka
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, AS CR, Prague 4, Czech Republic
    Mutat Res 669:13-9. 2009
    ..We found a correlation between adduct levels in the blood of mothers and newborns...
  26. ncbi Coke oven workers study: the effect of exposure and GSTM1 and NAT2 genotypes on DNA adduct levels in white blood cells and lymphocytes as determined by 32P-postlabelling
    B Binkova
    Laboratory of Genetic Ecotoxicology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Mutat Res 416:67-84. 1998
    ....
  27. ncbi DNA adducts in human placenta as related to air pollution and to GSTM1 genotype
    J Topinka
    Laboratory of Genetic Ecotoxicology, Regional Institute of Hygiene of Central Bohemia, Czech Academy of Sciences, Prague, Czech Republic
    Mutat Res 390:59-68. 1997
    ....
  28. ncbi PAH-DNA adducts in environmentally exposed population in relation to metabolic and DNA repair gene polymorphisms
    Blanka Binkova
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine AS CR and Health Institute of Central Bohemia, Videnska 1083, 14220 Prague, Czech Republic
    Mutat Res 620:49-61. 2007
    ....
  29. ncbi In vitro genotoxicity of PAH mixtures and organic extract from urban air particles part I: acellular assay
    B Binkova
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, AS CR and Health Institute of Central Bohemia, Videnska 1083, 142 20 Prague 4, Czech Republic
    Mutat Res 620:114-22. 2007
    ..Our findings support a hypothesis that a relatively limited number of EOM components is responsible for a major part of its genotoxicity detectable as DNA adducts by (32)P-postlabelling...
  30. ncbi Spontaneous level of chromosomal aberrations in peripheral blood lymphocytes of control individuals of the Czech Republic population
    P Rossner
    Laboratory of Genetic Toxicology, National Institute of Public Health, Prague, Czech Republic
    Toxicol Lett 96:137-42. 1998
    ..C.) with age. We found 1.10% AB.C. (N = 551) in newborns; 0.71% AB.C. (N = 105) in the group 5-6 yr; 1.20% (N = 1,734) in the group 7-15 yr; 1.25% AB.C. (N = 239) in the group 16-19 yr and 1.59% (N = 2,801) in the group 20-63 yr...
  31. doi The impact of air pollution on the levels of micronuclei measured by automated image analysis
    A Rossnerova
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague 4, Czech Republic
    Mutat Res 669:42-7. 2009
    ..Our work is the first human biomonitoring study focused on the measurement of MN by automated image analysis for assessing chromosomal damage as a result of environmental mutagen exposure...
  32. ncbi The effect of dibenzo[a,1]pyrene and benzo[a]pyrene on human diploid lung fibroblasts: the induction of DNA adducts, expression of p53 and p21(WAF1) proteins and cell cycle distribution
    B Binkova
    Laboratory of Genetic Ecotoxicology, Regional Institute of Hygiene of Central Bohemia, c o Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic
    Mutat Res 471:57-70. 2000
    ..Whether the S phase delay observed in our study is beneficial for the survival of the cells remains to be further established...
  33. ncbi The effects of exposure to different clastogens on the pattern of chromosomal aberrations detected by FISH whole chromosome painting in occupationally exposed individuals
    O Beskid
    Institute of Experimental Medicine AS CR and Health Institute of Central Bohemia, Videnska 1083, 142 20 Prague 4, Czech Republic
    Mutat Res 594:20-9. 2006
    ..Smoking led to significantly increased F(G)/100 (P < 0.05), but did not affect the pattern of chromosomal aberrations. Our results seem to indicate that different carcinogens may induce a different pattern of chromosomal aberrations...
  34. ncbi Frequency of chromosomal aberrations in Prague mothers and their newborns
    A Rossnerova
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, AS CR, v v i, 142 20 Prague 4, Czech Republic
    Mutat Res 699:29-34. 2010
    ..The mother's age affected the level of aberrations in newborns: the group of children born to older mothers (31-40 years) had significantly increased F(G)/100 levels...
  35. ncbi Genotoxicity and embryotoxicity of urban air particulate matter collected during winter and summer period in two different districts of the Czech Republic
    B Binkova
    Laboratory of Genetic Ecotoxicology, Regional Institute of Hygiene of Central Bohemia, c o Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20, Prague 4, Czech Republic
    Mutat Res 440:45-58. 1999
    ..This is the first report in which the biological activities of complex mixtures in short-term assays with remarkably different endpoints such as DNA adduct formation and embryotoxicity have been compared...
  36. ncbi Temporal variation in the genotoxic potential of urban air particulate matter
    O Sevastyanova
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, vvi and Health Institute of Central Bohemia, Videnska 1083, Prague, Czech Republic
    Mutat Res 649:179-86. 2008
    ....
  37. doi Oxidative damage to biological macromolecules in Prague bus drivers and garagemen: impact of air pollution and genetic polymorphisms
    Yana Bagryantseva
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine v v i, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Praha 4, Czech Republic
    Toxicol Lett 199:60-8. 2010
    ..In contrast, GSTM1 null variant seemed to protect DNA integrity...
  38. pmc The impact of polycyclic aromatic hydrocarbons and fine particles on pregnancy outcome
    J Dejmek
    Laboratory of Genetic Ecotoxicology, Regional Institute of Hygiene of Central Bohemia and Institute of Experimental Medicine, Academy of Science of the Czech Republic, Prague, Czech Republic
    Environ Health Perspect 108:1159-64. 2000
    ..The results prove that exposure to c-PAHs in early gestation may influence fetal growth. The particulate matter-IUGR association observed earlier may be at least partly explained by the presence of c-PAHs on particle surfaces...
  39. ncbi Chromosomal aberrations in peripheral lymphocytes of children as biomarkers of environmental exposure and life style
    P Rossner
    Laboratory of Genetic Toxicology, National Institute of Public Health, Prague, Czech Republic
    Toxicol Lett 134:79-85. 2002
    ..Based on our experience, we believe that monitoring the spontaneous level of chromosomal aberrations in children over 5 year periods may be used to examine the general changes in environmental pollution in larger geographic areas...
  40. ncbi Chromosomal aberrations by fluorescence in situ hybridization (FISH)--Biomarker of exposure to carcinogenic PAHs
    Olena Beskid
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine AS CR, v v i, and Health Institute of Central Bohemia, Prague, Czech Republic
    Mutat Res 620:62-70. 2007
    ..According to the original hypothesis it is expected that the most important group of chemicals responsible for the biological activity of air pollution represent c-PAHs...
  41. doi Genetic variability of HVRII mtDNA in cord blood and respiratory morbidity in children
    J Schmuczerova
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, v v i, Videnska 1083, 142 20 Prague 4, Czech Republic
    Mutat Res 666:1-7. 2009
    ..46, p=0.030, 95% CI 1.04-2.06). The results indicate that genetic polymorphisms in mtDNA may be an important factor not only for various types of cancers and neurodegenerative diseases, but also for respiratory morbidity in children...
  42. pmc Fetal growth and maternal exposure to particulate matter during pregnancy
    J Dejmek
    Laboratory of Genetic Ecotoxicology, Regional Institute of Hygiene of Central Bohemia and Institute of Experimental Medicine, Prague, Videnska, Czech Republic
    Environ Health Perspect 107:475-80. 1999
    ..Seasonal factors, one of the other possible explanations, is less probable. More investigation is required to examine these findings and alternative explanations...
  43. ncbi In vitro genotoxicity of PAH mixtures and organic extract from urban air particles part II: human cell lines
    O Sevastyanova
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic
    Mutat Res 620:123-34. 2007
    ....
  44. ncbi Oxidative and nitrosative stress markers in bus drivers
    Pavel Rossner
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine AS CR and Health Institute of Central Bohemia, Videnska 1083, 142 20 Prague, Czech Republic
    Mutat Res 617:23-32. 2007
    ..32, p<0.001). Both oxidative and nitrosative stress markers positively correlated with PM2.5 and PM10 exposure. In conclusion, our study indicates that exposure to PM2.5 and PM10 results in increased oxidative and nitrosative stress...
  45. doi Effect of maternal tobacco smoke exposure on the placental transcriptome
    H Bruchova
    Department of Molecular Genetics, Institute of Hematology and Blood Transfusion, Prague, Czech Republic
    Placenta 31:186-91. 2010
    ..Functional annotation analysis suggested processes and pathways affected by tobacco smoke exposure that may represent molecular mechanisms of smoke-induced placental abnormalities...
  46. ncbi HVRII of mtDNA in cord blood cells of newborn children and in their saliva 10 years later
    J Schmuczerova
    Department of Developmental Genotoxicology, Institute of Experimental Medicine AS CR, Prague, Czech Republic
    Folia Biol (Praha) 57:26-9. 2011
    ....
  47. ncbi DNA adduct formation in mammalian cell cultures by polycyclic aromatic hydrocarbons (PAH) and nitro-PAH in coke oven emission extract
    J Topinka
    Laboratory of Genetic Ecotoxicology, Regional Institute of Hygiene of Central Bohemia and Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20, Prague 4, Czech Republic
    Mutat Res 419:91-105. 1998
    ..Using DNA adduct analysis in rat hepatocytes (+/-pretreatment with TCDD) and in NCI-H322 and in V79NH cells offers a promising approach to determine the genotoxic activity of PAH and nitro-PAH in any complex environmental samples...
  48. ncbi DNA adducts and human atherosclerotic lesions
    B Binkova
    Laboratory of Genetic Ecotoxicology, Regional Institute of Hygiene of Central Bohemia, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Int J Hyg Environ Health 204:49-54. 2001
    ..Furthermore, atherogenesis and carcinogenesis may share a similar etiology, i.e. genotoxic action of environmental chemicals...
  49. doi Effect of vitamin levels on biomarkers of exposure and oxidative damage-the EXPAH study
    R J Sram
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine of Academy AS CR and Health Institute of Central Bohemia, Prague, Czech Republic
    Mutat Res 672:129-34. 2009
    ..This could contribute to the protective role of such a dietary pattern on cancer risk. The protective effect of dietary vitamins is less evident in smokers...
  50. ncbi The distribution of major lymphocyte subsets in cord blood is associated with its pH
    M Dostal
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, Czech Academy of Sciences and Regional Institute of Hygiene of Central Bohemia, 142 20, Prague, Czech Republic
    Clin Biochem 34:119-24. 2001
    ..To assess in venous cord blood the distribution of major lymphocyte subsets according to pH and medications used during labor...
  51. ncbi [Environmental pollution and childhood morbidity]
    M Dostal
    Ustav experimentální medicíny AV CR, Praha
    Cas Lek Cesk 140:529-32. 2001
    ..Comparisons were drawn between children living in district Teplice, known for its high air pollution, and those living in Practice, the district with consistently lower particulate and SO2 exposures...
  52. ncbi [Parental smoking and exposure of pre-school children to tobacco smoke]
    M Dostal
    Ustav experimentální medicíny AV CR a Zdravotní ustav Stredoceského kraje, Praha
    Cas Lek Cesk 145:313-8; discussion 319-21. 2006
    ..During studies on the health of children aged 3 or 4.5 years in Teplice and Prachatice districts of the Czech Republic, we focused also on the extent of smoking in the families and exposure of children to environmental tobacco smoke...
  53. ncbi Impact of air pollution and genotype variability on DNA damage in Prague policemen
    Bozena Novotna
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic and Health Institute of Central Bohemia, Videnska 1083, 142 20 Praha 4, Czech Republic
    Toxicol Lett 172:37-47. 2007
    ..In contrast, GSTM1-null and vitamin C tended rather to protect DNA integrity...
  54. ncbi Air pollution by carcinogenic PAHs and plasma levels of p53 and p21(WAF1) proteins
    Pavel Rossner
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, AS CR and Health Institute of Central Bohemia, Prague, Czech Republic
    Mutat Res 620:34-40. 2007
    ..In conclusion our results suggest that p53 and p21(WAF1) proteins plasma levels may be useful biomarkers of c-PAHs environmental exposure...
  55. ncbi The DNA repair gene XPD/ERCC2 polymorphisms Arg156Arg (exon 6) and Lys751Gln (exon 23) are closely associated
    Jan Topinka
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic
    Toxicol Lett 172:85-9. 2007
    ....
  56. ncbi Environmental tobacco smoke exposure in children in two districts of the Czech Republic
    Miroslav Dostal
    Institute of Experimental Medicine AS CR, Prague, Czech Republic
    Int J Hyg Environ Health 211:318-25. 2008
    ..6% vs. 27.8%). Our results suggest that in the Czech Republic, children under 5 years of age are significantly exposed to tobacco smoke and that more effective regulatory measures are needed to decrease the prevalence of smoking...
  57. ncbi Seasonal variability of oxidative stress markers in city bus drivers. Part II. Oxidative damage to lipids and proteins
    Pavel Rossner
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, AS CR vvi, Videnska 1083, Prague, Czech Republic
    Mutat Res 642:21-7. 2008
    ..Lipid peroxidation was positively correlated with c-PAHs and PM exposure; protein oxidation correlated negatively and was highest in summer suggesting another factor(s) affecting protein carbonyl levels...
  58. doi An acellular assay to assess the genotoxicity of complex mixtures of organic pollutants bound on size segregated aerosol. Part I: DNA adducts
    Jan Topinka
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, v v i, Prague, Czech Republic
    Toxicol Lett 198:304-11. 2010
    ..001). It may be concluded that the analysis of total DNA adducts induced in an acellular assay with/without metabolic activation represents a relatively simple method to assess the genotoxic potential of various complex mixtures...
  59. ncbi Seasonal variability of oxidative stress markers in city bus drivers. Part I. Oxidative damage to DNA
    Pavel Rossner
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, AS CR vvi, Videnska 1083, Prague 4, Czech Republic
    Mutat Res 642:14-20. 2008
    ..5 and PM10 levels, measured by stationary monitors during a 3-day period before urine collection, as the only factors significantly affecting 8-oxodG levels, while the levels of c-PAHs had no significant influence...
  60. doi Comparison of the health of Roma and non-Roma children living in the district of Teplice
    Miroslav Dostal
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Int J Public Health 55:435-41. 2010
    ..To compare the morbidity of 66 Roma and 466 non-Roma children born and living in a diffused type of habitation in the district of Teplice...
  61. doi An acellular assay to assess the genotoxicity of complex mixtures of organic pollutants bound on size segregated aerosol. Part II: oxidative damage to DNA
    Pavel Rossner
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine AS CR, v v i, Videnska 1083, 142 20 Prague, Czech Republic
    Toxicol Lett 198:312-6. 2010
    ..523, p=0.08). In conclusion, we showed that organic extracts of PM were able to induce oxidative damage to DNA in vitro; this ability was increased after S9 metabolic activation of EOM and with decreasing sizes of PM...
  62. pmc Fecundability and parental exposure to ambient sulfur dioxide
    J Dejmek
    Laboratory of Genetic Ecotoxicology, Regional Institute of Hygiene of Central Bohemia and Institute of Experimental Medicine, Academy of Sciences of Czech Republic, Prague
    Environ Health Perspect 108:647-54. 2000
    ..Alternative explanations of our results are also possible...
  63. ncbi Comparison of p53 levels in lymphocytes and in blood plasma of nuclear power plant workers
    Pavel Rossner
    Laboratory of Genetic Ecotoxicology, Health Institute of Central Bohemia and Institute of Experimental Medicine AS CR, Videnska 1083, 14220 Prague, Czech Republic
    Mutat Res 556:55-63. 2004
    ..The results of measurement p53 levels in lymphocytes suggest that this biomarker could reflect the short-term as well as long-term effects of low doses IR. Its impact on human health should be further explored...
  64. ncbi Urinary 8-oxodeoxyguanosine levels in children exposed to air pollutants
    Vlasta Svecova
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine AS CR, v v i, Videnska 1083, 142 20 Prague 4, Czech Republic
    Mutat Res 662:37-43. 2009
    ..The obtained results indicate that 8-oxodG is a sensitive biomarker for measuring the exposure of children to air pollution...