Anna Ligasová

Summary

Affiliation: Academy of Sciences of the Czech Republic
Country: Czech Republic

Publications

  1. pmc Secretory glands in cercaria of the neuropathogenic schistosome Trichobilharzia regenti - ultrastructural characterization, 3-D modelling, volume and pH estimations
    Anna Ligasová
    Department of Parasitology, Charles University, Prague, Czech Republic
    Parasit Vectors 4:162. 2011
  2. pmc Organization of human replicon: singles or zipping couples?
    Anna Ligasová
    Laboratory of Cell Biology, Institute of Experimental Medicine, v v i, Academy of Sciences of the Czech Republic, Videnska 1083, 14200 Prague 4, Czech Republic
    J Struct Biol 165:204-13. 2009
  3. pmc In situ reverse transcription: the magic of strength and anonymity
    Anna Ligasová
    Laboratory of Cell Biology, Institute of Experimental Medicine, v v i, AS CR, Videnska 1083, 142 20 Prague 4, Czech Republic
    Nucleic Acids Res 38:e167. 2010
  4. pmc Most anti-BrdU antibodies react with 2'-deoxy-5-ethynyluridine -- the method for the effective suppression of this cross-reactivity
    Radek Liboska
    Oligonucleotide Group, Institute of Organic Chemistry and Biochemistry, ASCR, v v i, Prague, Czech Republic
    PLoS ONE 7:e51679. 2012
  5. ncbi request reprint Electron microscopy of DNA replication in 3-D: evidence for similar-sized replication foci throughout S-phase
    Karel Koberna
    Department of Cell Biology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Albertov 4, CZ 12800 Prague 2, Czech Republic
    J Cell Biochem 94:126-38. 2005
  6. pmc Atomic scissors: a new method of tracking the 5-bromo-2'-deoxyuridine-labeled DNA in situ
    Anna Ligasová
    Department of Molecular Cytology and Cytometry, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
    PLoS ONE 7:e52584. 2012
  7. ncbi request reprint Tracking DNA and RNA sequences at high resolution
    Dusan Cmarko
    Institute of Cellular Biology and Pathology, 1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
    Methods Mol Biol 1117:343-66. 2014

Collaborators

Detail Information

Publications7

  1. pmc Secretory glands in cercaria of the neuropathogenic schistosome Trichobilharzia regenti - ultrastructural characterization, 3-D modelling, volume and pH estimations
    Anna Ligasová
    Department of Parasitology, Charles University, Prague, Czech Republic
    Parasit Vectors 4:162. 2011
    ..mansoni. In the context of different histolytic enzymes used by these two species, we focused also on the estimations of gland volumes and pH in T. regenti...
  2. pmc Organization of human replicon: singles or zipping couples?
    Anna Ligasová
    Laboratory of Cell Biology, Institute of Experimental Medicine, v v i, Academy of Sciences of the Czech Republic, Videnska 1083, 14200 Prague 4, Czech Republic
    J Struct Biol 165:204-13. 2009
    ..Moreover, our data enabled to suggest a new model of the organization of replicated DNA. According to this model, replisome couples produce loop with the associated arms in the form of four tightly associated 30nm fibers...
  3. pmc In situ reverse transcription: the magic of strength and anonymity
    Anna Ligasová
    Laboratory of Cell Biology, Institute of Experimental Medicine, v v i, AS CR, Videnska 1083, 142 20 Prague 4, Czech Republic
    Nucleic Acids Res 38:e167. 2010
    ..When the high pressure freezing technique followed by the freeze substitution is employed for the cell's preparation, the ratio is higher than 80...
  4. pmc Most anti-BrdU antibodies react with 2'-deoxy-5-ethynyluridine -- the method for the effective suppression of this cross-reactivity
    Radek Liboska
    Oligonucleotide Group, Institute of Organic Chemistry and Biochemistry, ASCR, v v i, Prague, Czech Republic
    PLoS ONE 7:e51679. 2012
    ..The use of hydrochloric acid resulted in a significant increase of the non-specific signal. In the case of the second method, no such effect was observed...
  5. ncbi request reprint Electron microscopy of DNA replication in 3-D: evidence for similar-sized replication foci throughout S-phase
    Karel Koberna
    Department of Cell Biology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Albertov 4, CZ 12800 Prague 2, Czech Republic
    J Cell Biochem 94:126-38. 2005
    ..3-D reconstruction of serial sections reveals a network-like organization of the RS in early-S-phase and confirms that numerous smaller-sized replication foci comprise the larger RS characteristic of late-S-phase...
  6. pmc Atomic scissors: a new method of tracking the 5-bromo-2'-deoxyuridine-labeled DNA in situ
    Anna Ligasová
    Department of Molecular Cytology and Cytometry, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
    PLoS ONE 7:e52584. 2012
    ..The alternative presently-used methods result in protein denaturation and/or extensive DNA cleavage followed by the DNA-bound proteins peeling off...
  7. ncbi request reprint Tracking DNA and RNA sequences at high resolution
    Dusan Cmarko
    Institute of Cellular Biology and Pathology, 1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
    Methods Mol Biol 1117:343-66. 2014
    ..In this respect, the technique enabling the enzymatic detection of the polyadenylated RNA sequences is described in detail. ..