Vladimir Kren

Summary

Affiliation: Academy of Sciences of the Czech Republic
Country: Czech Republic

Publications

  1. ncbi request reprint beta-Glucosylation of chitooligomers by galactosyltransferase
    V Kren
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Biotransformation, Prague, Czech Republic
    Carbohydr Res 305:517-23. 1997
  2. ncbi request reprint Chemistry of silybin
    D Biedermann
    Institute of Microbiology AS CR, Centre of Biocatalysis and Biotransformation, Videnska 1083, Prague 4, CZ 14220, Czech Republic
    Nat Prod Rep 31:1138-57. 2014
  3. pmc Structure of the dimeric N-glycosylated form of fungal beta-N-acetylhexosaminidase revealed by computer modeling, vibrational spectroscopy, and biochemical studies
    Rudiger Ettrich
    Laboratory of High Performance Computing, Institute of Systems Biology and Ecology of the Academy of Sciences of the Czech Republic and Institute of Physical Biology of USB, Zámek136, Nove Hrady, Czech Republic
    BMC Struct Biol 7:32. 2007
  4. ncbi request reprint Pergolide, terguride and N,N'-spacer-linked oligomers of both interact with 5-HT2A receptors of rat tail artery
    V Kren
    Institute of Microbiology, Laboratory of Biotransformation, Academy of Sciences of the Czech Republic, Prague 4, Czech Republic
    Physiol Res 53:35-43. 2004
  5. ncbi request reprint Clustered ergot alkaloids modulate cell-mediated cytotoxicity
    Vladimir Kren
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 142 20 4, Prague, Czech Republic
    Bioorg Med Chem 10:415-24. 2002
  6. doi request reprint Sweet antibiotics - the role of glycosidic residues in antibiotic and antitumor activity and their randomization
    Vladimir Kren
    Centre of Biocatalysis and Biotransformation, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    FEMS Microbiol Rev 32:858-89. 2008
  7. ncbi request reprint Glycosides in medicine: "The role of glycosidic residue in biological activity"
    V Kren
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Biotransformation, Videnska 1083, Prague, CZ 142 20, Czech Republic
    Curr Med Chem 8:1303-28. 2001
  8. ncbi request reprint Chemoenzymatic preparation of silybin beta-glucuronides and their biological evaluation
    V Kren
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Biotransformation, Prague, Czech Republic
    Drug Metab Dispos 28:1513-7. 2000
  9. doi request reprint Purification and characterization of heterologously expressed nitrilases from filamentous fungi
    Alena Petříčková
    Institute of Microbiology, Centre of Biocatalysis and Biotransformation, Prague, Czech Republic
    Appl Microbiol Biotechnol 93:1553-61. 2012
  10. ncbi request reprint Purification and characterization of a nitrilase from Aspergillus niger K10
    Ondrej Kaplan
    Institute of Microbiology, Centre of Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague, Czech Republic
    Appl Microbiol Biotechnol 73:567-75. 2006

Collaborators

Detail Information

Publications95

  1. ncbi request reprint beta-Glucosylation of chitooligomers by galactosyltransferase
    V Kren
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Biotransformation, Prague, Czech Republic
    Carbohydr Res 305:517-23. 1997
    ..beta-Glucosylated products were used in binding studies with NKR-P1A protein cloned from rat natural killer cells...
  2. ncbi request reprint Chemistry of silybin
    D Biedermann
    Institute of Microbiology AS CR, Centre of Biocatalysis and Biotransformation, Videnska 1083, Prague 4, CZ 14220, Czech Republic
    Nat Prod Rep 31:1138-57. 2014
    ..This review covers articles dealing with silybin chemistry and also summarizes all the derivatives prepared. ..
  3. pmc Structure of the dimeric N-glycosylated form of fungal beta-N-acetylhexosaminidase revealed by computer modeling, vibrational spectroscopy, and biochemical studies
    Rudiger Ettrich
    Laboratory of High Performance Computing, Institute of Systems Biology and Ecology of the Academy of Sciences of the Czech Republic and Institute of Physical Biology of USB, Zámek136, Nove Hrady, Czech Republic
    BMC Struct Biol 7:32. 2007
    ..The fungal beta-N-acetylhexosaminidases are robust enzymes commonly used in chemoenzymatic syntheses of oligosaccharides. The enzyme from Aspergillus oryzae was purified and its sequence was determined...
  4. ncbi request reprint Pergolide, terguride and N,N'-spacer-linked oligomers of both interact with 5-HT2A receptors of rat tail artery
    V Kren
    Institute of Microbiology, Laboratory of Biotransformation, Academy of Sciences of the Czech Republic, Prague 4, Czech Republic
    Physiol Res 53:35-43. 2004
    ..4). Oligomers of terguride showed 5 to 50-fold lower affinity. It is concluded that pergolide and terguride show a high affinity for 5-HT2A receptors, but dimerization (oligomerization) of both drugs fails to increase affinity...
  5. ncbi request reprint Clustered ergot alkaloids modulate cell-mediated cytotoxicity
    Vladimir Kren
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 142 20 4, Prague, Czech Republic
    Bioorg Med Chem 10:415-24. 2002
    ..This effect can be reversed by higher oligomerization of the respective alkaloids...
  6. doi request reprint Sweet antibiotics - the role of glycosidic residues in antibiotic and antitumor activity and their randomization
    Vladimir Kren
    Centre of Biocatalysis and Biotransformation, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    FEMS Microbiol Rev 32:858-89. 2008
    ..The new enzymatic methodology 'glycorandomization' enabled the preparation of glycoside libraries and opened up new ways to prepare optimized or entirely novel glycoside antibiotics...
  7. ncbi request reprint Glycosides in medicine: "The role of glycosidic residue in biological activity"
    V Kren
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Biotransformation, Videnska 1083, Prague, CZ 142 20, Czech Republic
    Curr Med Chem 8:1303-28. 2001
    ..The physiological role of the glycosyl and structure-activity relations (SAR) in the glycosidic moiety (-ies) are discussed...
  8. ncbi request reprint Chemoenzymatic preparation of silybin beta-glucuronides and their biological evaluation
    V Kren
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Biotransformation, Prague, Czech Republic
    Drug Metab Dispos 28:1513-7. 2000
    ..This is first pharmacological study using optically pure silybin...
  9. doi request reprint Purification and characterization of heterologously expressed nitrilases from filamentous fungi
    Alena Petříčková
    Institute of Microbiology, Centre of Biocatalysis and Biotransformation, Prague, Czech Republic
    Appl Microbiol Biotechnol 93:1553-61. 2012
    ..5 μmol min(-1) mg(-1) protein, respectively), and hydrolyzed also (R,S)-mandelonitrile with higher K(m) values. Significant amounts of amides were only formed by the G. moniliformis nitrilase from phenylacetonitrile and 4-cyanopyridine...
  10. ncbi request reprint Purification and characterization of a nitrilase from Aspergillus niger K10
    Ondrej Kaplan
    Institute of Microbiology, Centre of Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague, Czech Republic
    Appl Microbiol Biotechnol 73:567-75. 2006
    ..The enzyme stability was markedly improved in the presence of D: -sorbitol or xylitol (20% w/v each). p-Hydroxymercuribenzoate and heavy metal ions were the most powerful inhibitors of the enzyme...
  11. ncbi request reprint Novel double-congenic strain reveals effects of spontaneously hypertensive rat chromosome 2 on specific lipoprotein subfractions and adiposity
    Ondrej Seda
    Institute of Biology and Medical Genetics of the First Faculty of Medicine of Charles University and the General Teaching Hospital, Prague, Czech Republic
    Physiol Genomics 27:95-102. 2006
    ..For the first time, the chromosome 2 segments of SHR origin are shown to influence other than blood pressure-related features of metabolic syndrome or to be involved in relevant nutrigenomic interactions...
  12. ncbi request reprint Synthetic N-acetyl-D-glucosamine based fully branched tetrasaccharide, a mimetic of the endogenous ligand for CD69, activates CD69+ killer lymphocytes upon dimerization via a hydrophilic flexible linker
    Anna Kovalová
    Institute of Organic Chemistry and Biochemistry, vvi, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    J Med Chem 53:4050-65. 2010
    ..This compound is thus a candidate for carbohydrate-based immunomodulators with promising antitumor potential...
  13. doi request reprint Enzymatic synthesis of dimeric glycomimetic ligands of NK cell activation receptors
    Anna Drozdová
    Institute of Microbiology, Academy of Sciences of Czech Republic, Videnska 1083, CZ 14220 Praha 4, Czech Republic
    Carbohydr Res 346:1599-609. 2011
    ....
  14. ncbi request reprint Fluorescent labelled thiourea-bridged glycodendrons
    Pavel Krist
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic
    Chembiochem 5:445-52. 2004
    ..These studies are useful for probing the mechanism of action and fate of artificial ligands and the cell receptors involved...
  15. doi request reprint Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal β-N-acetylhexosaminidase
    Helena Ryšlavá
    Department of Biochemistry, Faculty of Science, Charles University Prague, Prague, Czech Republic
    FEBS J 278:2469-84. 2011
    ..oxalicum is unique among the family of fungal hexosaminidases...
  16. pmc Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10
    Ondrej Kaplan
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    BMC Biotechnol 11:2. 2011
    ..Nitrilases are typically heterogeneous in their quaternary structures, forming short spirals and extended filaments, these features making their structural studies difficult...
  17. ncbi request reprint Enzymatic preparation of silybin phase II metabolites: sulfation using aryl sulfotransferase from rat liver
    Katerina Purchartová
    Institute of Microbiology, Laboratory of Biotransformation, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 14220, Prague, Czech Republic
    Appl Microbiol Biotechnol 97:10391-8. 2013
    ..The sulfated product was isolated by solid phase extraction and its structure was characterized by HRMS and NMR. Sulfation reaction of silybin appeared strictly stereoselective; only silybin B was sulfated by AstIV...
  18. ncbi request reprint A chemoenzymatic route to mannosamine derivatives bearing different N-acyl groups
    Veronika Kristová
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Biotransformation, Videnska 1083, CZ 142 20 Prague 4, Czech Republic
    J Biotechnol 115:157-66. 2005
    ..Its activity was slightly inhibited by the addition of 1 mM Al3+, Ca2+, Co2+, Cu2+, Mn2+ or Zn2+ and activated by 1 mM Mg2+. The concentrated enzyme is highly stable at 4 degrees C in the presence of 0.1 M ammonium sulfate...
  19. doi request reprint [Erratum] Effects of N-acetyl-glucosamine-coated glycodendrimers as biological modulators in the B16F10 melanoma model in vivo
    Luca Vannucci
    Laboratory of Natural Cell Immunity, Department of Immunology and Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic, 14220 Prague 4, Czech Republic
    Int J Oncol 44:1410. 2014
    ..the original article was published in the International Journal of Oncology 23: 285-296, 2003]. ..
  20. doi request reprint Production of Aspergillus niger β-mannosidase in Pichia pastoris
    Barbora Fliedrová
    Laboratory of Biotransformation, Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, Prague, Czech Republic
    Protein Expr Purif 85:159-64. 2012
    ..28 and 0.44 mmol/l for p-nitrophenyl β-d-mannopyranoside, pI 4.2 and 4.0, and their pH optima were at pH 4.5 and 5.5 and 65°C, respectively)...
  21. doi request reprint Facile production of Aspergillus niger α-N-acetylgalactosaminidase in yeast
    Hynek Mrázek
    Laboratory of Protein Architecture, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Protein Expr Purif 81:106-14. 2012
    ..N-Glycosylation was confirmed at six of the eight potential N-glycosylation sites in both wild type and recombinant α-GalNAc-ase...
  22. ncbi request reprint Fungal nitrilases as biocatalysts: Recent developments
    Ludmila Martinkova
    Centre of Biocatalysis and Biotransformation, Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 142 20 Prague, Czech Republic
    Biotechnol Adv 27:661-70. 2009
    ..Operational stability was examined using continuous stirred membrane bioreactors. Fungal nitrilases appear promising for biocatalytic applications and biodegradation of nitrile environmental contaminants...
  23. ncbi request reprint Pharmacogenetic model of retinoic acid-induced dyslipidemia and insulin resistance
    Michaela Krupková
    Institute of Biology and Medical Genetics, The First Faculty of Medicine, Charles University, General Teaching Hospital, Prague, Czech Republic
    Pharmacogenomics 10:1915-27. 2009
    ....
  24. ncbi request reprint N-acetyl-D-glucosamine substituted calix[4]arenes as stimulators of NK cell-mediated antitumor immune response
    Karel Krenek
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Praha 4, Czech Republic
    Carbohydr Res 342:1781-92. 2007
    ..These calix-sugar compounds were superior to the previously tested PAMAM-GlcNAc(8)5...
  25. doi request reprint Genome mining for the discovery of new nitrilases in filamentous fungi
    Ondrej Kaplan
    Centre of Biocatalysis and Biotransformation, Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague, Czech Republic
    Biotechnol Lett 33:309-12. 2011
    ..This was much less than the activities of the recombinant enzymes derived from A. niger and N. crassa that had activities of 2500 and 2700 U l(-1) culture, respectively, with phenylacetonitrile as substrate...
  26. ncbi request reprint Identification of a mutation in ADD1/SREBP-1 in the spontaneously hypertensive rat
    M Pravenec
    Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic
    Mamm Genome 12:295-8. 2001
    ....
  27. ncbi request reprint Synthesis of LacdiNAc-terminated glycoconjugates by mutant galactosyltransferase--a way to new glycodrugs and materials
    Pavla Bojarová
    Institute of Microbiology, Center for Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Czech Republic
    Glycobiology 19:509-17. 2009
    ..A synthetic bivalent tethered di-LacdiNAc is the best currently known precipitation agent for both of these receptors and has promising potential for the development of immunoactive glycodrugs...
  28. doi request reprint Preparatory production of quercetin-3-β-D-glucopyranoside using alkali-tolerant thermostable α-L-rhamnosidase from Aspergillus terreus
    Lenka Weignerova
    Institute of Microbiology, Center for Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Bioresour Technol 115:222-7. 2012
    ..Isoquercitrin can be produced to a very high purity (98%) in multikilogram amounts, without any quercetin and directly applicable in nutraceuticals...
  29. ncbi request reprint Preparative production and separation of 2-acetamido-2-deoxymannopyranoside-containing saccharides using borate-saturated polyolic exclusion gels
    Lucie Petrásková
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Biotransformation, Videnska 1083, 142 20 Prague 4, Czech Republic
    J Chromatogr A 1127:126-36. 2006
    ..NMR studies of the interactions of N-acetylhexosamines with borate confirmed the importance of a proper stereochemical arrangement of acetamido sugars for their interactions with borate anions...
  30. doi request reprint 4-Deoxy-substrates for beta-N-acetylhexosaminidases: how to make use of their loose specificity
    Kristyna Slamova
    Centre of Biocatalysis and Biotransformation, Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 14220 Praha 4, Czech Republic
    Glycobiology 20:1002-9. 2010
    ....
  31. ncbi request reprint Inhibition of GlcNAc-processing glycosidases by C-6-azido-NAG-thiazoline and its derivatives
    Jana Krejzová
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Praha 4, Czech Republic
    Molecules 19:3471-88. 2014
    ....
  32. ncbi request reprint Oxidised derivatives of silybin and their antiradical and antioxidant activity
    Radek Gažák
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Biotransformation, Videnska 1083, CZ 142 20 Prague, Czech Republic
    Bioorg Med Chem 12:5677-87. 2004
    ..On the other hand, 2,3-dehydrosilybinic acid is a fairly soluble derivative with antilipoperoxidation and antiradical activities better than that of silybin...
  33. ncbi request reprint Differential effects of 5-HT3 receptor antagonist on lipid profile in spontaneously hypertensive rat and chromosome 8 congenic strain
    Michaela Krupková
    Institute of Biology and Medical Genetics, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 33:43-9. 2012
    ....
  34. ncbi request reprint Molecular mechanisms of silybin and 2,3-dehydrosilybin antiradical activity--role of individual hydroxyl groups
    Radek Gažák
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 142 20 Prague, Czech Republic
    Free Radic Biol Med 46:745-58. 2009
    ..The pivotal role of the 3-OH group in the radical-scavenging activity of 2 was confirmed through the formation of another type of dimer from its selectively methylated derivative...
  35. doi request reprint Synthesis and antiangiogenic activity of new silybin galloyl esters
    Radek Gažák
    Centre of Biotransformation and Biocatalysis, Institute of Microbiology Academy of Sciences of the Czech Republic, Videnska 1083, Prague 4, CZ 142 20, Czech Republic
    J Med Chem 54:7397-407. 2011
    ..As with silybin itself, the B isomer of 7-O-galloylsilybin was more active than the A isomer...
  36. doi request reprint The α-galactosidase type A gene aglA from Aspergillus niger encodes a fully functional α-N-acetylgalactosaminidase
    Natallia Kulik
    Center for Biocatalysis and Biotransformation, Institute of Systems Biology and Ecology, Academy of Sciences of the Czech Republic, Zamek 136, CZ37333 Nové Hrady, Czech Republic
    Glycobiology 20:1410-9. 2010
    ..Thus, we provide evidence that the α-galactosidase type A gene aglA from A. niger in fact encodes a fully functional α-N-acetylgalactosaminidase using a retaining mechanism...
  37. ncbi request reprint Y-chromosome transfer induces changes in blood pressure and blood lipids in SHR
    V Kren
    Institute of Biology and Medical Genetics, First Medical Faculty, Charles University, Prague, Czech Republic
    Hypertension 37:1147-52. 2001
    ..Thus, genetic variation in genes on the Y chromosome may contribute to variation in blood pressure and lipid levels and may influence the risk for cardiovascular disease...
  38. ncbi request reprint Linkage mapping of the Fos cellular oncogene (Fos) to rat chromosome 6 and its possible role in the regulation of compensatory renal growth
    M Pravenec
    Institute of Physiology, Academy of Sciences of the Czech Republic, Prague
    Folia Biol (Praha) 44:151-3. 1998
    ..Despite relatively wide confidence intervals for mapping QTLs with modest effects in a small population of RI strains, this finding probably excludes the Fos as a positional candidate for CRG...
  39. pmc Genetic isolation of a region of chromosome 8 that exerts major effects on blood pressure and cardiac mass in the spontaneously hypertensive rat
    V Kren
    Institute of Biology, First Medical Faculty, Charles University, Prague, Czech Republic
    J Clin Invest 99:577-81. 1997
    ..BN-Lx rats...
  40. doi request reprint Antioxidant and antiviral activities of silybin fatty acid conjugates
    Radek Gažák
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 142 20 Prague, Czech Republic
    Eur J Med Chem 45:1059-67. 2010
    ..The acyl chain length is an important prerequisite for both biological activities, as they improved with increasing length of the acyl moiety...
  41. ncbi request reprint Effects of N-acetyl-glucosamine-coated glycodendrimers as biological modulators in the B16F10 melanoma model in vivo
    Luca Vannucci
    Institute of Microbiology, Czech Academy of Sciences, 14220 Prague 4, Czech Republic
    Int J Oncol 23:285-96. 2003
    ..Our data suggest that well-defined multivalent neo-glycoconjugates can stimulate an antitumor immune response engaging both innate and acquired immunity...
  42. doi request reprint Sequencing, cloning and high-yield expression of a fungal β-N-acetylhexosaminidase in Pichia pastoris
    Kristyna Slamova
    Institute of Microbiology, Center for Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 14220, Prague 4, Czech Republic
    Protein Expr Purif 82:212-7. 2012
    ..This is the first example of the overexpression of a fungal β-N-acetylhexosaminidase by a single-cell producer in liquid medium. It represents a promising solution for wider biotechnological applications of this outstanding enzyme...
  43. ncbi request reprint Biotransformations with nitrilases
    Ludmila Martinkova
    Laboratory of Biotransformation, Institute of Microbiology of the Academy of Science of the Czech Republic, 142 20 Prague, Czech Republic
    Curr Opin Chem Biol 14:130-7. 2010
    ..Such processes have been developed, for instance, for the synthesis of (S)-mandelic acid, (S)-mandeloamide or glycolic acid...
  44. pmc Mapping of quantitative trait loci for blood pressure and cardiac mass in the rat by genome scanning of recombinant inbred strains
    M Pravenec
    Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic
    J Clin Invest 96:1973-8. 1995
    ....
  45. ncbi request reprint Synthesis of chitooligomer-based glycoconjugates and their binding to the rat natural killer cell activation receptor NKR-P1
    T Semenuk
    Institute of Microbiology, Laboratory of Biotransformation, Academy of Sciences of the Czech Republic, , CZ-142 20 Prague 4, Czech Republic
    Glycoconj J 18:817-26. 2001
    ..In analogy with our previous observations with GlcNAc clustered on protein or PAMAM backbones the synthetic chitooligomer clusters should provide considerably better ligands in the in vivo antitumor treatment...
  46. ncbi request reprint Access to bifunctionalized biomolecular platforms using oxime ligation
    Karel Krenek
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 14220 Praha 4, Czech Republic
    Carbohydr Res 393:9-14. 2014
    ..The well-defined structure of these conjugates was confirmed by high-resolution mass spectrometry. ..
  47. doi request reprint Enzymatic glycosylation of multivalent scaffolds
    Pavla Bojarová
    Institute of Microbiology, Center for Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 142 20, Prague 4, Czech Republic
    Chem Soc Rev 42:4774-97. 2013
    ..Though there is still a long way until the Nature's ideal of multivalent glycans is achievable in the laboratory, the sketched pathways to multivalent glycostructures open tremendous possibilities for the future glycobiological research...
  48. doi request reprint Laboratory evolution of an epoxide hydrolase - towards an enantioconvergent biocatalyst
    Michael Kotik
    Laboratory of Biotransformation, Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic
    J Biotechnol 156:1-10. 2011
    ....
  49. ncbi request reprint Biotransformation of heterocyclic dinitriles by Rhodococcus erythropolis and fungal nitrilases
    Vojtech Vejvoda
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeská 1083, CZ 142 20, Prague 4, Czech Republic
    Biotechnol Lett 29:1119-24. 2007
    ..3,4-Pyridinedicarbonitrile (3a) and 2,3-pyrazinedicarbonitrile (4a) were inferior substrates of nitrile hydratase and nitrilase...
  50. ncbi request reprint Selection and screening for enzymes of nitrile metabolism
    Ludmila Martinkova
    Centre of Biocatalysis and Biotransformation, Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic
    J Biotechnol 133:318-26. 2008
    ..However, the potential of these principles, in particular spectrophotometric and fluorimetric methods, will be probably further exploited and adapted to multiwell plate and robotic systems...
  51. ncbi request reprint Transgenic rescue of defective Cd36 ameliorates insulin resistance in spontaneously hypertensive rats
    M Pravenec
    Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic
    Nat Genet 27:156-8. 2001
    ..Our results provide direct evidence that Cd36 deficiency can promote defective insulin action and disordered fatty-acid metabolism in spontaneous hypertension...
  52. ncbi request reprint Mapping of quantitative trait loci for seminal vesicle mass and litter size to rat chromosome 8
    V Zidek
    Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic
    J Reprod Fertil 116:329-33. 1999
    ....
  53. ncbi request reprint Chemo-enzymatic synthesis of silybin and 2,3-dehydrosilybin dimers
    Eva Vavríková
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 142 20 Prague 4, Czech Republic
    Molecules 19:4115-34. 2014
    ..Silybin dimer was more cytotoxic than the parent compound and in the case of 2,3-dehydrosilybin its dimer showed weaker cytotoxicity than the monomer. ..
  54. doi request reprint Expression, characterization and homology modeling of a novel eukaryotic GH84 β-N-acetylglucosaminidase from Penicillium chrysogenum
    Kristyna Slamova
    Laboratory of Biotransformation, Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Praha 4, Czech Republic Electronic address
    Protein Expr Purif 95:204-10. 2014
    ..This work helped to increase the knowledge on the scarcely studied glycosidase family and revealed a new type of eukaryotic β-N-acetylglucosaminidase. ..
  55. pmc Facile synthesis of nitrophenyl 2-acetamido-2-deoxy-α-D-mannopyranosides from ManNAc-oxazoline
    Karel Krenek
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 142 20, Prague, Czech Republic
    Beilstein J Org Chem 8:428-32. 2012
    ..The synthetic pathway employs the glycosylation of phenol with ManNAc oxazoline, followed by nitration of the aromatic moiety yielding a separable mixture of the o- and p-nitrophenyl derivative in a 2:3 ratio...
  56. doi request reprint Synthesis and biological activity of glycosyl-1H-1,2,3-triazoles
    Kristyna Slamova
    Centre of Biocatalysis and Biotransformation, Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 14220, Prague, Czech Republic
    Bioorg Med Chem Lett 20:4263-5. 2010
    ..Beta-GlcNAc-triazole was furthermore established to act as a strong ligand of rat and human natural killer cell activating receptors...
  57. ncbi request reprint Glycosidases in carbohydrate synthesis: when organic chemistry falls short
    Pavla Bojarová
    Academy of Sciences of the Czech Republic, Institute of Microbiology, Center of Biocatalysis and Biotransformation, Videnska 1083, CZ 142 20 Praha 4, Czech Republic
    Chimia (Aarau) 65:65-70. 2011
    ..Products of glycosidase-catalyzed synthesis are applicable in a range of areas such as immunology, therapy of Alzheimer's or Parkinson's diseases and the synthesis of neoglycoproteins...
  58. doi request reprint Biodegradation of tetrabromobisphenol A by oxidases in basidiomycetous fungi and estrogenic activity of the biotransformation products
    Bronislava Uhnáková
    Institute of Microbiology, Laboratory of Biotransformation, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic
    Bioresour Technol 102:9409-15. 2011
    ..2006), laccase can provide a simple means of preparing these metabolites for toxicity studies. Products 1 and 2 exhibited estrogenic effects, unlike TBBPA, but lower cell toxicity...
  59. ncbi request reprint Genetic isolation of quantitative trait loci for blood pressure development and renal mass on chromosome 5 in the spontaneously hypertensive rat
    M Pravenec
    Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic
    Physiol Res 52:285-9. 2003
    ..The findings of the current study demonstrate that gene(s) with major effects on blood pressure and renal mass exist in the differential segment of chromosome 5 trapped within the new SHR.BN congenic strain...
  60. ncbi request reprint Genetic isolation of a blood pressure quantitative trait locus on chromosome 2 in the spontaneously hypertensive rat
    M Pravenec
    Institute of Physiology and Center for Integrated Genomics, Czech Academy of Sciences, Prague, Czech Republic
    J Hypertens 19:1061-4. 2001
    ..The objective of the current study was to directly test for the presence of a blood pressure QTL on rat chromosome 2...
  61. ncbi request reprint Genetic analysis of cardiovascular risk factor clustering in spontaneous hypertension
    M Pravenec
    Institute of Physiology, Academy of Sciences of the Czech Republic, Prague
    Folia Biol (Praha) 46:233-40. 2000
    ....
  62. ncbi request reprint Chromosomal mapping of a major quantitative trait locus regulating compensatory renal growth in the rat
    M Pravenec
    Institute of Physiology, Czech Academy of Sciences, Czech Republic
    J Am Soc Nephrol 11:1261-5. 2000
    ....
  63. ncbi request reprint Isotretinoin and fenofibrate induce adiposity with distinct effect on metabolic profile in a rat model of the insulin resistance syndrome
    L Sedova
    Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University, Prague, Czech Republic
    Int J Obes Relat Metab Disord 28:719-25. 2004
    ....
  64. ncbi request reprint Genetic analysis of "metabolic syndrome" in the spontaneously hypertensive rat
    M Pravenec
    Department of Genetics of Model Diseases, Institute of Physiology, Academy of Sciences of the Czech Republic and Center for Integrated Genomics, Prague, Czech Republic
    Physiol Res 53:S15-22. 2004
    ....
  65. ncbi request reprint N-glycosylated catalytic unit meets O-glycosylated propeptide: complex protein architecture in a fungal hexosaminidase
    O Plíhal
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 14220 Praha 4, Czech Republic
    Biochem Soc Trans 32:764-5. 2004
    ..Deglycosylation experiments revealed that the N-glycosylation increased the stability and solubility of the enzyme. In contrast, O-glycosylated propeptide was necessary to attain the full enzymic activity...
  66. ncbi request reprint A 14-gene region of rat chromosome 8 in SHR-derived polydactylous congenic substrain affects muscle-specific insulin resistance, dyslipidaemia and visceral adiposity
    O Seda
    Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University, Prague, Czech Republic
    Folia Biol (Praha) 51:53-61. 2005
    ....
  67. ncbi request reprint Assignment of the cellular retinol binding protein 1 (Rbp1) and hepatic lipase (Lipc) genes to rat chromosome 8
    M Pravenec
    Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Folia Biol (Praha) 43:97-9. 1997
    ..The current findings extend homologies between rat chromosome 8, mouse chromosome 9, and human chromosomes 3q and 15q...
  68. ncbi request reprint HXB/Ipcv and BXH/Cub recombinant inbred strains of the rat: strain distribution patterns of 632 alleles
    M Pravenec
    Institute of Physiology, Academy of Sciences of the Czech Republic, Prague
    Folia Biol (Praha) 45:203-15. 1999
    ..The RI strains were phenotyped in multiple hemodynamic and metabolic traits. In the current study, we describe strain distribution patterns of 632 genetic markers...
  69. ncbi request reprint Linkage mapping of the interleukin 1beta converting enzyme (Il1bc) and the glutamate receptor subunit KA1 (Grik4) genes to rat chromosome 8
    M Pravenec
    Institute of Physiology, Academy of Sciences of the Czech Republic, Prague
    Folia Biol (Praha) 44:107-9. 1998
    ..The current results demonstrate that rat chromosome 8 is largely homologous to mouse chromosome 9...
  70. ncbi request reprint Hydrolysis of lysergamide to lysergic acid by Rhodococcus equi A4
    L Martinkova
    Laboratory of Biotransformation, Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 142 20 4, Prague, Czech Republic
    J Biotechnol 84:63-6. 2001
    ..From a mixture of lysergamide and its epimer isolysergamide, Rhodococcus equi A4 containing amidase preferentially hydrolyzed lysergamide into lysergic acid...
  71. ncbi request reprint GlcNAc-terminated glycodendrimers form defined precipitates with the soluble dimeric receptor of rat natural killer cells, sNKR-P1A
    K Bezouska
    Department of Biochemistry, Faculty of Science, Charles University Prague, Phaha, Czech Republic
    FEBS Lett 426:243-7. 1998
    ..These results provide new tools for further studies on carbohydrate recognition by NKR-P1A...
  72. ncbi request reprint Silybin and silymarin--new effects and applications
    Vladimir Kren
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 149:29-41. 2005
    ..New derivatives of silybin open new ways to its therapeutic applications. Pharmacology dealing with optically pure silybin diastereomers may suggest new mechanisms of its action...
  73. ncbi request reprint Large propeptides of fungal beta-N-acetylhexosaminidases are novel enzyme regulators that must be intracellularly processed to control activity, dimerization, and secretion into the extracellular environment
    Ondřej Plíhal
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Praha 4, Czech Republic
    Biochemistry 46:2719-34. 2007
    ..This novel regulatory mechanism enables the control of the catalytic activity and architecture of the secreted enzyme according to the needs of the producing cell at various stages of its growth cycle...
  74. ncbi request reprint Enzymatic processing of bioactive glycosides from natural sources
    Lenka Weignerova
    Institute of Microbiology, Centre for Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, Vídeíská 1083, CZ 142 20 Praha 4, Czech Republic
    Top Curr Chem 295:121-46. 2010
    ..g., in nutraceuticals. This chapter concentrates on naturally occurring glycosides used in medicine but also in the food and flavor industry (e.g., sweeteners). Both "classical" and modern methods will be discussed...
  75. doi request reprint Biodegradation potential of the genus Rhodococcus
    Ludmila Martinkova
    Centre of Biocatalysis and Biotransformation, Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 142 20 Prague 4, Czech Republic
    Environ Int 35:162-77. 2009
    ..Examples of regulatory mechanisms for the expression of the catabolic genes are given. The strains that efficiently degrade the compounds in question are highlighted and examples of their use in biodegradation processes are presented...
  76. doi request reprint β-N-acetylhexosaminidase: what's in a name…?
    Kristyna Slamova
    Institute of Microbiology, Centre of Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, Videnska 1083, Prague 4, Czech Republic
    Biotechnol Adv 28:682-93. 2010
    ..Thus, they have proved to be versatile biosynthetic tools for the preparation of both natural and modified hexosaminides under mild conditions with good yields...
  77. ncbi request reprint Toward an optimal oligosaccharide ligand for rat natural killer cell activation receptor NKR-P1
    P Krist
    Institute of Microbiology, Laboratory of Biotransformation, Academy of Sciences of Czech Republic, , CZ-142 20, Prague 4, Czech Republic
    Biochem Biophys Res Commun 287:11-20. 2001
    ....
  78. ncbi request reprint Metabolic characterization of insulin resistance syndrome feature loci in three brown Norway-derived congenic strains
    O Seda
    Institute of Biology and Medical Genetics, 1st Medical Faculty, Charles University, Prague, Czech Republic
    Folia Biol (Praha) 48:81-8. 2002
    ..The set of defined congenic strains provides a possibility of assessing individual features of such a complex phenotype...
  79. ncbi request reprint Rosiglitazone improves insulin resistance, lipid profile and promotes adiposity in a genetic model of metabolic syndrome X
    O Seda
    Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University, Prague, Czech Republic
    Folia Biol (Praha) 48:237-41. 2002
    ..In consent with previously published data we suggest the current pharmacogenetic study as a further proof of substantial influence of genetic background on the physiological outcome of TZD therapy...
  80. ncbi request reprint Recombinant inbred and congenic strains of the rat for genetic analysis of limb morphogenesis
    V Kren
    Department of Biology, 1st Medical Faculty, Charles University, Prague, Czech Republic
    Folia Biol (Praha) 42:159-66. 1996
    ..5 to 0.6). The verification of all these findings is in progress by means of double congenic strains which, in addition to the Lx gene, carry differential chromosome segments with putative modifiers...
  81. ncbi request reprint PXO set of recombinant inbred strains of the rat: a new strain distribution pattern containing 448 markers
    D Kemlink
    Institute of Biology and Medical Genetics, 1st Medical Faculty, Charles University, Prague, Czech Republic
    Folia Biol (Praha) 49:165-76. 2003
    ..The SDP of PXO strains with polymorphic markers arranged in approximately 5 cM intervals is ready for the association analysis and interval mapping in interconnection with the SDP of HXB/BXH strains...
  82. ncbi request reprint Isoquercitrin: pharmacology, toxicology, and metabolism
    Katerina Valentova
    Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, CZ 775 15 Olomouc, Czech Republic Laboratory of Biotransformation, Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, Prague 4 CZ 142 20, Czech Republic Centre of Drug Dietary Supplements Interactions and Nutrigenetics, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, CZ 775 15 Olomouc, Czech Republic Electronic address
    Food Chem Toxicol 68:267-82. 2014
    ..With respect to the safety, affordability and beneficial pharmacological activities, highly pure isoquercitrin is a prospective substance for food supplementation. ..
  83. pmc Re-Evaluation of Binding Properties of Recombinant Lymphocyte Receptors NKR-P1A and CD69 to Chemically Synthesized Glycans and Peptides
    Daniel Rozbeský
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, Prague 4 CZ14220, Czech Republic
    Int J Mol Sci 15:1271-83. 2014
    ....
  84. ncbi request reprint Induction and characterization of an unusual alpha-D-galactosidase from Talaromyces flavus
    Pavla Simerska
    Institute of Microbiology, Academy of Sciences of Czech Republic, Videnska 1083, CZ 142 20 Prague 4, Czech Republic
    J Biotechnol 128:61-71. 2007
    ..Moreover, it was active on polymeric substrates (locust bean gum, guar gum) and significantly inhibited by alpha-D-galactopyranosyl azide, D-galactose, D-xylose, melibiose, methyl alpha- and beta-D-galactopyranoside and lactose...
  85. doi request reprint Glycosidases: a key to tailored carbohydrates
    Pavla Bojarová
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Center of Biocatalysis and Biotransformation, Videnska 1083, CZ 142 20, Praha 4, Czech Republic
    Trends Biotechnol 27:199-209. 2009
    ..Selective glycosidase inhibitors and highly specific glycosidases are finding attractive applications in biomedicine, biology and proteomics...
  86. ncbi request reprint Enzymatic glycosylation using 6-O-acylated sugar donors and acceptors: beta-N-acetylhexosaminidase-catalysed synthesis of 6-O,N,N'-triacetylchitobiose and 6'-O,N,N'-triacetylchitobiose
    L Husakova
    Laboratory of Biotransformation, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague
    Carbohydr Res 331:143-8. 2001
    ....
  87. ncbi request reprint Application of selectively acylated glycosides for the alpha-galactosidase-catalyzed synthesis of disaccharides
    P Simerska
    Laboratory of Biotransformation, Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia
    Folia Microbiol (Praha) 48:329-37. 2003
    ..2-Methylpropan-2-ol and acetone (increasing the solubility of the modified substrate acceptors and displaying the minimum impairment of the activity and stability of the enzyme) were used as co-solvents in transglycosylation reactions...
  88. ncbi request reprint Enzymatic glycosylation of lincomycin
    L Weignerova
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague
    Biosci Biotechnol Biochem 65:1897-9. 2001
    ..Compound 1 was glycosylated using jack bean alpha-mannosidase to produce 7-O-alpha-D-mannopyranosyl-lincomycin (2)...
  89. ncbi request reprint Purification and characterization of the enantioselective nitrile hydratase from Rhodococcus equi A4
    I Prepechalová
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague
    Appl Microbiol Biotechnol 55:150-6. 2001
    ..The addition of 5% (v/v) of n-hexane, n-heptane, isooctane, n-hexadecane, pristane and methanol increased the E-value for the enzymatic hydration of 2-(6-methoxynaphthyl)propionitrile...
  90. ncbi request reprint Fungal beta-N-acetylhexosaminidases with high beta-N-acetylgalactosaminidase activity and their use for synthesis of beta-GalNAc-containing oligosaccharides
    Lenka Weignerova
    Laboratory of Biotransformation, Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, Prague 4, CZ 142 20, Czech Republic
    Carbohydr Res 338:1003-8. 2003
    ....
  91. pmc Pharmacogenetic interaction between dexamethasone and Cd36-deficient segment of spontaneously hypertensive rat chromosome 4 affects triacylglycerol and cholesterol distribution into lipoprotein fractions
    Michaela Krupková
    Institute of Biology and Medical Genetics, Charles University, General Teaching Hospital, Prague, Czech Republic
    Lipids Health Dis 9:38. 2010
    ..In summary, we report a pharmacogenetic interaction between limited genomic segment with mutated Cd36 gene and dexamethasone-induced glucose intolerance and triacylglycerol and cholesterol redistribution into lipoprotein fractions...
  92. ncbi request reprint Silybin and silymarin--new and emerging applications in medicine
    Radek Gažák
    Laboratory of Biotransformation, Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídezská 1083, CZ 142 20 Prague, Czech Republic
    Curr Med Chem 14:315-38. 2007
    ..Significance of silymarin and its components in the medicine is clearly indicated by an exponential growth of publications on this topic--over 800 papers in the last 5 years...
  93. ncbi request reprint Hemodynamic characterization of recombinant inbred strains: twenty years later
    Jaroslav Kunes
    Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Hypertens Res 31:1659-68. 2008
    ..Our study confirms the validity of RI strains for the further genetic and physiological research of hypertension...
  94. ncbi request reprint The CD36 protein functions as an immunogenic domain of the RT8 alloantigen
    P Mlejnek
    Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic
    Eur J Immunogenet 30:325-7. 2003
    ....
  95. ncbi request reprint Differential linkage of triglyceride and glucose levels on rat chromosome 4 in two segregating rat populations
    O Seda
    Institute of Biology and Medical Genetics, First Faculty of Medcine, Charles University, Prague, Czech Republic
    Folia Biol (Praha) 49:223-6. 2003
    ..The Cd36/Fat and Il-6 genes are the preliminary positional candidates for the observed effect...