Jana Humpolickova

Summary

Affiliation: Academy of Sciences of the Czech Republic
Country: Czech Republic

Publications

  1. doi request reprint Dynamic saturation optical microscopy: employing dark-state formation kinetics for resolution enhancement
    Jana Humpolickova
    J Heyrovsky Institute of Physical Chemistry of the Academy of Sciences of the Czech Republic, v v i Dolejskova 3, Prague 8, 18223, Czech Republic
    Phys Chem Chem Phys 12:12457-65. 2010
  2. pmc Optical saturation as a versatile tool to enhance resolution in confocal microscopy
    Jana Humpolickova
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Biophys J 97:2623-9. 2009
  3. ncbi request reprint Solvent relaxation in phospholipid bilayers: principles and recent applications
    Piotr Jurkiewicz
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 3, CZ 18223, Prague 8, Czech Republic
    J Fluoresc 15:883-94. 2005
  4. pmc Dynamics and size of cross-linking-induced lipid nanodomains in model membranes
    Martin Stefl
    Department of Biophysical Chemistry, J Heyrovsky Institute of Physical Chemistry of the Academy of Sciences of the Czech Republic, Dolejskova, Prague, Czech Republic
    Biophys J 102:2104-13. 2012
  5. pmc Probing diffusion laws within cellular membranes by Z-scan fluorescence correlation spectroscopy
    Jana Humpolickova
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Biophys J 91:L23-5. 2006
  6. ncbi request reprint Fluorescence lifetime correlation spectroscopy reveals compaction mechanism of 10 and 49 kbp DNA and differences between polycation and cationic surfactant
    Jana Humpolickova
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, v v i, Dolejskova 2155 3, CZ 18223, Prague 8, Czech Republic
    J Phys Chem B 112:16823-9. 2008
  7. ncbi request reprint Lipid diffusion in giant unilamellar vesicles is more than 2 times faster than in supported phospholipid bilayers under identical conditions
    Magdalena Przybylo
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 3, 18223 Prague 8, Czech Republic
    Langmuir 22:9096-9. 2006
  8. doi request reprint Effect of heavy water on phospholipid membranes: experimental confirmation of molecular dynamics simulations
    Lenka Beranova
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, vvi, Prague 8, Czech Republic
    Phys Chem Chem Phys 14:14516-22. 2012
  9. doi request reprint Advanced imaging of cellular signaling events
    Marek Cebecauer
    Department of Biophysics, J Heyrovsky Institute of Physical Chemistry ASCR, Prague, Czech Republic
    Methods Enzymol 505:273-89. 2012
  10. pmc Limitations of electronic energy transfer in the determination of lipid nanodomain sizes
    Radek Sachl
    J Heyrovsky Institute of Physical Chemistry, v v i, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Biophys J 101:L60-2. 2011

Collaborators

Detail Information

Publications12

  1. doi request reprint Dynamic saturation optical microscopy: employing dark-state formation kinetics for resolution enhancement
    Jana Humpolickova
    J Heyrovsky Institute of Physical Chemistry of the Academy of Sciences of the Czech Republic, v v i Dolejskova 3, Prague 8, 18223, Czech Republic
    Phys Chem Chem Phys 12:12457-65. 2010
    ..By mapping the intensity dependent decay kinetics, enhanced resolution images can be obtained. Generally, any dark state of fluorescent molecules can be employed in DSOM. Here, we focus our attention on triplet state formation...
  2. pmc Optical saturation as a versatile tool to enhance resolution in confocal microscopy
    Jana Humpolickova
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Biophys J 97:2623-9. 2009
    ..We demonstrate our approach imaging GFP-labeled protein patches within the plasma membrane of yeast cells...
  3. ncbi request reprint Solvent relaxation in phospholipid bilayers: principles and recent applications
    Piotr Jurkiewicz
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 3, CZ 18223, Prague 8, Czech Republic
    J Fluoresc 15:883-94. 2005
    ..Moreover, limitations and potential problems are discussed...
  4. pmc Dynamics and size of cross-linking-induced lipid nanodomains in model membranes
    Martin Stefl
    Department of Biophysical Chemistry, J Heyrovsky Institute of Physical Chemistry of the Academy of Sciences of the Czech Republic, Dolejskova, Prague, Czech Republic
    Biophys J 102:2104-13. 2012
    ....
  5. pmc Probing diffusion laws within cellular membranes by Z-scan fluorescence correlation spectroscopy
    Jana Humpolickova
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Biophys J 91:L23-5. 2006
    ..e., by performing a Z-scan...
  6. ncbi request reprint Fluorescence lifetime correlation spectroscopy reveals compaction mechanism of 10 and 49 kbp DNA and differences between polycation and cationic surfactant
    Jana Humpolickova
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, v v i, Dolejskova 2155 3, CZ 18223, Prague 8, Czech Republic
    J Phys Chem B 112:16823-9. 2008
    ..The conclusions drawn are furthermore supported by two standard techniques, dynamic light scattering (DLS), and fluorescence correlation spectroscopy (FCS)...
  7. ncbi request reprint Lipid diffusion in giant unilamellar vesicles is more than 2 times faster than in supported phospholipid bilayers under identical conditions
    Magdalena Przybylo
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 3, 18223 Prague 8, Czech Republic
    Langmuir 22:9096-9. 2006
    ..The results obtained revealed that the lipid mobility in free-standing bilayers (D=7.8+/-0.8 microm2 s-1) is significantly higher than in the bilayer created on the solid support (mica) (D=3.1+/-0.3 microm2 s-1)...
  8. doi request reprint Effect of heavy water on phospholipid membranes: experimental confirmation of molecular dynamics simulations
    Lenka Beranova
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, vvi, Prague 8, Czech Republic
    Phys Chem Chem Phys 14:14516-22. 2012
    ..Together with the simulations our experiments provide a comprehensive, molecular view on the effect of D(2)O on phospholipid bilayers...
  9. doi request reprint Advanced imaging of cellular signaling events
    Marek Cebecauer
    Department of Biophysics, J Heyrovsky Institute of Physical Chemistry ASCR, Prague, Czech Republic
    Methods Enzymol 505:273-89. 2012
    ..Presented techniques provide a new level of detail one can learn about higher organization of signaling events in human cells...
  10. pmc Limitations of electronic energy transfer in the determination of lipid nanodomain sizes
    Radek Sachl
    J Heyrovsky Institute of Physical Chemistry, v v i, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Biophys J 101:L60-2. 2011
    ..Here the detection limits of FRET experiments employing several commonly used D/A pairs have been investigated...
  11. doi request reprint On mechanism of intermediate-sized circular DNA compaction mediated by spermine: contribution of fluorescence lifetime correlation spectroscopy
    Jana Humpolickova
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 3, Prague 8, Czech Republic
    J Fluoresc 18:679-84. 2008
    ..Furthermore, we show that it allows for observation of the equilibrium state transition dynamics...
  12. pmc The C-terminal domain of Brd2 is important for chromatin interaction and regulation of transcription and alternative splicing
    Jarmila Hnilicová
    Department of RNA Biology, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague 142 20, Czech Republic Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague 142 20, Czech Republic Max Planck Institute for Molecular Cell Biology and Genetics, Dresden 01307, Germany J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Prague 182 23, Czech Republic
    Mol Biol Cell 24:3557-68. 2013
    ..Finally, we show that bromodomains and the C-terminal domain are equally important for transcription and splicing regulation, which correlates with the role of these domains in Brd2 binding to chromatin. ..