Martin Hof

Summary

Affiliation: Academy of Sciences of the Czech Republic
Country: Czech Republic

Publications

  1. pmc Fluorescence Lifetime Correlation Spectroscopy (FLCS): Concepts, Applications and Outlook
    Peter Kapusta
    J Heyrovsky Institute of Physical Chemistry of ASCR, v v i, Dolejskova 3, 18223 Prague 8, Czech Republic
    Int J Mol Sci 13:12890-910. 2012
  2. pmc Förster Resonance Energy Transfer (FRET) between Heterogeneously Distributed Probes: Application to Lipid Nanodomains and Pores
    Radek Sachl
    Department of Biophysical Chemistry, Jaroslav Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 2155 3, Prague 8, 182 23, Czech Republic
    Int J Mol Sci 13:16141-56. 2012
  3. doi request reprint Effect of heavy water on phospholipid membranes: experimental confirmation of molecular dynamics simulations
    Lenka Beranova
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, vvi, Prague 8, Czech Republic
    Phys Chem Chem Phys 14:14516-22. 2012
  4. pmc Dynamics and size of cross-linking-induced lipid nanodomains in model membranes
    Martin Stefl
    Department of Biophysical Chemistry, J Heyrovsky Institute of Physical Chemistry of the Academy of Sciences of the Czech Republic, Dolejskova, Prague, Czech Republic
    Biophys J 102:2104-13. 2012
  5. doi request reprint Oxidation changes physical properties of phospholipid bilayers: fluorescence spectroscopy and molecular simulations
    Lenka Beranova
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, v v i, Dolejskova 3, 18223 Prague 8, Czech Republic
    Langmuir 26:6140-4. 2010
  6. doi request reprint Biophysics of lipid bilayers containing oxidatively modified phospholipids: insights from fluorescence and EPR experiments and from MD simulations
    Piotr Jurkiewicz
    Academy of Sciences of the Czech Republic, Prague 8, Czech Republic
    Biochim Biophys Acta 1818:2388-402. 2012
  7. ncbi request reprint Fluorescence lifetime correlation spectroscopy reveals compaction mechanism of 10 and 49 kbp DNA and differences between polycation and cationic surfactant
    Jana Humpolickova
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, v v i, Dolejskova 2155 3, CZ 18223, Prague 8, Czech Republic
    J Phys Chem B 112:16823-9. 2008
  8. doi request reprint Fluorescence lifetime tuning--a novel approach to study flip-flop kinetics in supported phospholipid bilayers
    Anna Kułakowska
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, v v i, Dolejskova 3, 18223 Prague 8, Czech Republic
    J Fluoresc 20:563-9. 2010
  9. ncbi request reprint Headgroup hydration and mobility of DOTAP/DOPC bilayers: a fluorescence solvent relaxation study
    Piotr Jurkiewicz
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 3, CZ 18223 Prague 8, Czech Republic
    Langmuir 22:8741-9. 2006
  10. ncbi request reprint Comprehensive portrait of cholesterol containing oxidized membrane
    Martin Stefl
    J Heyrovsky Institute of Physical Chemistry of the Academy of Sciences of the Czech Republic, v v i, Dolejskova 2155 3, 182 23 Prague 8, Czech Republic Electronic address
    Biochim Biophys Acta 1838:1769-76. 2014

Collaborators

Detail Information

Publications43

  1. pmc Fluorescence Lifetime Correlation Spectroscopy (FLCS): Concepts, Applications and Outlook
    Peter Kapusta
    J Heyrovsky Institute of Physical Chemistry of ASCR, v v i, Dolejskova 3, 18223 Prague 8, Czech Republic
    Int J Mol Sci 13:12890-910. 2012
    ..The generalization of the idea of FLCS paves the way for further promising application of the principle of statistical filtering of signals. Specifically, the idea of fluorescence spectral correlation spectroscopy is here outlined...
  2. pmc Förster Resonance Energy Transfer (FRET) between Heterogeneously Distributed Probes: Application to Lipid Nanodomains and Pores
    Radek Sachl
    Department of Biophysical Chemistry, Jaroslav Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 2155 3, Prague 8, 182 23, Czech Republic
    Int J Mol Sci 13:16141-56. 2012
    ..The experimental setup where donors and acceptors are excluded from the domains/pores should be avoided...
  3. doi request reprint Effect of heavy water on phospholipid membranes: experimental confirmation of molecular dynamics simulations
    Lenka Beranova
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, vvi, Prague 8, Czech Republic
    Phys Chem Chem Phys 14:14516-22. 2012
    ..Together with the simulations our experiments provide a comprehensive, molecular view on the effect of D(2)O on phospholipid bilayers...
  4. pmc Dynamics and size of cross-linking-induced lipid nanodomains in model membranes
    Martin Stefl
    Department of Biophysical Chemistry, J Heyrovsky Institute of Physical Chemistry of the Academy of Sciences of the Czech Republic, Dolejskova, Prague, Czech Republic
    Biophys J 102:2104-13. 2012
    ....
  5. doi request reprint Oxidation changes physical properties of phospholipid bilayers: fluorescence spectroscopy and molecular simulations
    Lenka Beranova
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, v v i, Dolejskova 3, 18223 Prague 8, Czech Republic
    Langmuir 26:6140-4. 2010
    ..Molecular dynamics simulations corroborate the experimental findings, providing at the same time a detailed molecular interpretation in terms of changes in bilayer structure and phospholipid orientation...
  6. doi request reprint Biophysics of lipid bilayers containing oxidatively modified phospholipids: insights from fluorescence and EPR experiments and from MD simulations
    Piotr Jurkiewicz
    Academy of Sciences of the Czech Republic, Prague 8, Czech Republic
    Biochim Biophys Acta 1818:2388-402. 2012
    ..This article is part of a Special Issue entitled: Oxidized phospholipids-their properties and interactions with proteins...
  7. ncbi request reprint Fluorescence lifetime correlation spectroscopy reveals compaction mechanism of 10 and 49 kbp DNA and differences between polycation and cationic surfactant
    Jana Humpolickova
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, v v i, Dolejskova 2155 3, CZ 18223, Prague 8, Czech Republic
    J Phys Chem B 112:16823-9. 2008
    ..The conclusions drawn are furthermore supported by two standard techniques, dynamic light scattering (DLS), and fluorescence correlation spectroscopy (FCS)...
  8. doi request reprint Fluorescence lifetime tuning--a novel approach to study flip-flop kinetics in supported phospholipid bilayers
    Anna Kułakowska
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, v v i, Dolejskova 3, 18223 Prague 8, Czech Republic
    J Fluoresc 20:563-9. 2010
    ..Moreover, we compare and discuss the flip-flop rates of the probes with respect to their lateral diffusion coefficients...
  9. ncbi request reprint Headgroup hydration and mobility of DOTAP/DOPC bilayers: a fluorescence solvent relaxation study
    Piotr Jurkiewicz
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 3, CZ 18223 Prague 8, Czech Republic
    Langmuir 22:8741-9. 2006
    ..Above this concentration, elevated lipid bilayer water penetration was observed...
  10. ncbi request reprint Comprehensive portrait of cholesterol containing oxidized membrane
    Martin Stefl
    J Heyrovsky Institute of Physical Chemistry of the Academy of Sciences of the Czech Republic, v v i, Dolejskova 2155 3, 182 23 Prague 8, Czech Republic Electronic address
    Biochim Biophys Acta 1838:1769-76. 2014
    ..In this view cholesterol seems to have a tendency to repair, rather than condense, the bilayer. ..
  11. pmc The C-terminal domain of Brd2 is important for chromatin interaction and regulation of transcription and alternative splicing
    Jarmila Hnilicová
    Department of RNA Biology, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague 142 20, Czech Republic Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague 142 20, Czech Republic Max Planck Institute for Molecular Cell Biology and Genetics, Dresden 01307, Germany J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Prague 182 23, Czech Republic
    Mol Biol Cell 24:3557-68. 2013
    ..Finally, we show that bromodomains and the C-terminal domain are equally important for transcription and splicing regulation, which correlates with the role of these domains in Brd2 binding to chromatin. ..
  12. ncbi request reprint Time-dependent stokes shifts of fluorescent dyes in the hydrophobic backbone region of a phospholipid bilayer: combination of fluorescence spectroscopy and ab initio calculations
    Jan Sykora
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 3, Prague 8, Czech Republic
    J Phys Chem B 111:5869-77. 2007
    ..A slowdown in solvent relaxation is observed upon moving toward the center of the bilayer. A mechanism similar to preferential solvation present in the mixture of a polar and nonpolar solvent is considered to explain the obtained data...
  13. doi request reprint Structure, dynamics, and hydration of POPC/POPS bilayers suspended in NaCl, KCl, and CsCl solutions
    Piotr Jurkiewicz
    Academy of Sciences of the Czech Republic, Prague 8, Czech Republic
    Biochim Biophys Acta 1818:609-16. 2012
    ..All these effects follow the same Hofmeister ordering as the cationic adsorption to the bilayer...
  14. pmc The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells
    Martina Huranová
    Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 142 20 Prague, Czech Republic
    J Cell Biol 191:75-86. 2010
    ..These results are consistent with the predictions of the step-wise model of spliceosome assembly and provide an estimate on the rate of splicing in human cells...
  15. ncbi request reprint Peripheral and integral membrane binding of peptides characterized by time-dependent fluorescence shifts: focus on antimicrobial peptide LAH₄
    Radek Macháň
    J Heyrovsky Institute of Physical Chemistry of ASCR, v v i, Dolejskova 3, Prague 8, CZ 18223, Czech Repulic
    Langmuir 30:6171-9. 2014
    ..In both cases, the addition of LAH4 does not lead to vesicle disintegration. The influence of negatively charged lipids on the bilayer permeation is also addressed. ..
  16. doi request reprint Real-time monitoring of melittin-induced pore and tubule formation from supported lipid bilayers and its physiological relevance
    Radek Macháň
    J Heyrovsky Institute of Physical Chemistry v v i, Academy of Sciences of the Czech Republic, Dolejskova 3, CZ18223 Prague, Czech Republic
    Chem Phys Lipids 163:200-6. 2010
    ..Flushing of the sample with excess of buffer removes most of the tubules, but it does not affect the pores. Further experiments performed under various conditions demonstrated reproducibility of both phenomena...
  17. ncbi request reprint Interactions of monovalent salts with cationic lipid bilayers
    Sarka Pokorna
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, v v i, Dolejskova 3, 18223 Prague 8, Czech Republic
    Faraday Discuss 160:341-58; discussion 389-403. 2013
    ..e. it increases local mobility at the lipid carbonyl level. We attribute this effect to the disruption of the hydrogen-bonded structure of the molecules of lipids and water caused by the presence of the adsorbed F-...
  18. pmc Limitations of electronic energy transfer in the determination of lipid nanodomain sizes
    Radek Sachl
    J Heyrovsky Institute of Physical Chemistry, v v i, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Biophys J 101:L60-2. 2011
    ..Here the detection limits of FRET experiments employing several commonly used D/A pairs have been investigated...
  19. ncbi request reprint Lipid diffusion in giant unilamellar vesicles is more than 2 times faster than in supported phospholipid bilayers under identical conditions
    Magdalena Przybylo
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 3, 18223 Prague 8, Czech Republic
    Langmuir 22:9096-9. 2006
    ..The results obtained revealed that the lipid mobility in free-standing bilayers (D=7.8+/-0.8 microm2 s-1) is significantly higher than in the bilayer created on the solid support (mica) (D=3.1+/-0.3 microm2 s-1)...
  20. pmc Probing diffusion laws within cellular membranes by Z-scan fluorescence correlation spectroscopy
    Jana Humpolickova
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Biophys J 91:L23-5. 2006
    ..e., by performing a Z-scan...
  21. ncbi request reprint Solvent relaxation in phospholipid bilayers: principles and recent applications
    Piotr Jurkiewicz
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 3, CZ 18223, Prague 8, Czech Republic
    J Fluoresc 15:883-94. 2005
    ..Moreover, limitations and potential problems are discussed...
  22. doi request reprint Dynamic saturation optical microscopy: employing dark-state formation kinetics for resolution enhancement
    Jana Humpolickova
    J Heyrovsky Institute of Physical Chemistry of the Academy of Sciences of the Czech Republic, v v i Dolejskova 3, Prague 8, 18223, Czech Republic
    Phys Chem Chem Phys 12:12457-65. 2010
    ..By mapping the intensity dependent decay kinetics, enhanced resolution images can be obtained. Generally, any dark state of fluorescent molecules can be employed in DSOM. Here, we focus our attention on triplet state formation...
  23. doi request reprint Formation of arenicin-1 microdomains in bilayers and their specific lipid interaction revealed by Z-scan FCS
    Radek Macháň
    J Heyrovsky Institute of Physical Chemistry v v i, Academy of Sciences of the Czech Republic, Dolejskova 2155 3, 182 23 Prague 8, Czech Republic
    Anal Bioanal Chem 399:3547-54. 2011
    ..Additionally, our FCS results imply that arenicin-1 exists in the form of oligomers and/or aggregates when interacting with membranes of both compositions...
  24. doi request reprint Numerical studies of the membrane fluorescent dyes dynamics in ground and excited states
    Justyna Barucha-Kraszewska
    Ustav fyzikální chemie J Heyrovského AV CR, v v i, Dolejskova 2155 3, 18223 Praha 8, Czech Republic
    Biochim Biophys Acta 1798:1724-34. 2010
    ..Four different relaxation time constants, corresponding to different relaxation processes, where observed when the dyes were embedded into the membrane...
  25. doi request reprint Accurate determination of the orientational distribution of a fluorescent molecule in a phospholipid membrane
    Stepán Timr
    Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nam 2, 16610 Prague 6, Czech Republic
    J Phys Chem B 118:855-63. 2014
    ..Such determinations enable rational development of a novel class of sensitive fluorescent optical probes, reporting on cellular events through changes in linear dichroism. ..
  26. ncbi request reprint Absence of ethanol-induced interdigitation in supported phospholipid bilayers on silica surfaces
    Adam Miszta
    J Heyrovsky Institute of Physical Chemistry v v i, Academy of Sciences of the Czech Republic, Dolejskova 3, Prague, Czech Republic
    Langmuir 24:19-21. 2008
    ..In addition, pure DOPC and DLPC membranes were studied. It is demonstrated that ethanol-induced membrane interdigitation, as demonstrated for free-standing bilayers, does not occur in these supported membranes...
  27. doi request reprint Absorption and fluorescence of PRODAN in phospholipid bilayers: a combined quantum mechanics and classical molecular dynamics study
    Lukasz Cwiklik
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, v v i, Prague, Czech Republic
    J Phys Chem A 115:11428-37. 2011
    ..Orientation of the excited dye in the lipid bilayer significantly depends on configuration. In the bilayer, the fluorescence spectrum can be regarded as a combination of emission from both planar and twisted structures...
  28. ncbi request reprint Fluorescence lifetime correlation spectroscopy combined with lifetime tuning: new perspectives in supported phospholipid bilayer research
    Ales Benda
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 3, 18223 Praha 8, Czech Republic
    Langmuir 22:9580-5. 2006
    ..Possible applications of this virtual two-channel measurement using single dye labeling as well as one detection channel are discussed...
  29. ncbi request reprint Di- and tri-oxalkyl derivatives of a boron dipyrromethene (BODIPY) rotor dye in lipid bilayers
    Marie Olšinová
    Department of Biophysical Chemistry, J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, v v i, Dolejskova 3, 18223 Prague, Czech Republic
    Phys Chem Chem Phys 16:10688-97. 2014
    ..The results presented herein also underline the importance of the detailed characterisation of fluorescent membrane dyes and provide a guide for future testing. ..
  30. ncbi request reprint Dynamics and hydration explain failed functional transformation in dehalogenase design
    Jan Sykora
    1 J Heyrovsky Institute of Physical Chemistry of the ASCR, v v i, Academy of Sciences of the Czech Republic, Prague, Czech Republic 2
    Nat Chem Biol 10:428-30. 2014
    ..Time-dependent fluorescence shifts and computer simulations revealed that dynamics and hydration at the tunnel mouth differ substantially between the redesigned and target dehalogenase. ..
  31. doi request reprint Are time-dependent fluorescence shifts at the tunnel mouth of haloalkane dehalogenase enzymes dependent on the choice of the chromophore?
    Mariana Amaro
    J Heyrovsky Institute of Physical Chemistry of the ASCR, v v i, Academy of Sciences of the Czech Republic, Dolejskova 3, 182 23 Praha, Czech Republic
    J Phys Chem B 117:7898-906. 2013
    ..Our study shows, for the first time, that TDFS may strongly depend on the choice of the chromophore, even though the identical region of a protein is explored. ..
  32. ncbi request reprint Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy
    Radek Macháň
    J Heyrovsky Institute of Physical Chemistry v v i, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Biochim Biophys Acta 1798:1377-91. 2010
    ..The recent FCS studies addressing those questions are reviewed and possible explanations of eventual discrepancies are mentioned...
  33. doi request reprint Lipopolythiourea/DNA interaction: a biophysical study
    Teresa Kral
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 3, Prague 8, Czech Republic
    Biophys Chem 148:68-73. 2010
    ..From previous in vitro experiments, we could conclude that the lipid leading to the more condensed state of DNA was also the more efficient to transfect cells...
  34. ncbi request reprint DNA-spermine and DNA-lipid aggregate formation visualized by fluorescence correlation spectroscopy
    Teresa Kral
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Chemotherapy 52:196-9. 2006
    ..Since diffusion coefficients are correlated with size and shape of the labeled species, FCS provides information on conformational changes in plasmids aggregates...
  35. doi request reprint Spectral analysis of doxorubicin accumulation and the indirect quantification of its DNA intercalation
    Ondrej Hovorka
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Eur J Pharm Biopharm 76:514-24. 2010
    ....
  36. doi request reprint On mechanism of intermediate-sized circular DNA compaction mediated by spermine: contribution of fluorescence lifetime correlation spectroscopy
    Jana Humpolickova
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 3, Prague 8, Czech Republic
    J Fluoresc 18:679-84. 2008
    ..Furthermore, we show that it allows for observation of the equilibrium state transition dynamics...
  37. doi request reprint Properties of mixed cationic membranes studied by fluorescence solvent relaxation
    Agnieszka Olzyńska
    J Heyrovsky Institute of Physical Chemistry of the ASCR, v v i, Dolejskova 3, Prague 8, 18223, Czech Republic
    J Fluoresc 18:925-8. 2008
    ..Langmuir 22:8741-8749, 2006), for DMPC/DMTAP system it was found at 45 mol% of DMTAP, which agrees with the literature. Both membranes increased their hydration upon increased cationic lipid content...
  38. ncbi request reprint Combination of ellipsometry, laser scanning microscopy and Z-scan fluorescence correlation spectroscopy elucidating interaction of cryptdin-4 with supported phospholipid bilayers
    Adam Miszta
    J Heyrovsky Institute of Physical Chemistry v v i, Academy of Sciences of the Czech Republic, Czech Republic
    J Pept Sci 14:503-9. 2008
    ..It seems likely that differences in peptide mode of action, readily distinguished using these combined experimental methods, are related to the distinctive beta-sheet and alpha-helical structures of the respective peptides...
  39. doi request reprint Photoactive oriented films of layered double hydroxides
    Kamil Lang
    Institute of Inorganic Chemistry, v v i, Academy of Sciences of the Czech Republic, 250 68 Rez, Czech Republic
    Phys Chem Chem Phys 10:4429-34. 2008
    ..The hybrid films contained homogeneously distributed porphyrin molecules with preserved photophysical properties such as fluorescence, triplet state formation, and energy transfer, thus forming singlet oxygen...
  40. pmc Recent developments in fluorescence correlation spectroscopy for diffusion measurements in planar lipid membranes
    Radek Macháň
    J Heyrovsky Institute of Physical Chemistry of ASCR, Prague, Czech Republic
    Int J Mol Sci 11:427-57. 2010
    ....
  41. ncbi request reprint Influence of the curvature on the water structure in the headgroup region of phospholipid bilayer studied by the solvent relaxation technique
    Jan Sykora
    J Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 3, CZ 18223 Prague 8, Czech Republic
    Chem Phys Lipids 135:213-21. 2005
    ..DTMAC (first time used in SR) shows a broad distribution of locations along the z-axis. A substantial amount of the coumarin chromophores face bulk water. No effect of curvature on SR probed by DTMAC is detectable...
  42. pmc In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer
    Martina Huranová
    Department of RNA Biology, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, CZ 14220 Prague 4, Czech Republic
    RNA 15:2063-71. 2009
    ....
  43. doi request reprint Fluorescence spectroscopy studies of HEK293 cells expressing DOR-Gi1α fusion protein; the effect of cholesterol depletion
    Jana Brejchová
    Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic
    Biochim Biophys Acta 1808:2819-29. 2011
    ..The biophysical state of hydrophobic plasma (cell) membrane interior should be regarded as regulatory factor of DOR-signaling cascade...