Maria G Guzman

Summary

Affiliation: Ciudad de La Habana
Country: Cuba

Publications

  1. ncbi request reprint Do escape mutants explain rapid increases in dengue case-fatality rates within epidemics?
    M G Guzman
    Institute of Tropical Medicine Pedro Kouri, Havana, Cuba
    Lancet 355:1902-3. 2000
  2. ncbi request reprint [Thirty years after the Cuban dengue hemorrhagic epidemic occurred in 1981]
    C María G Guzmán Tirado
    Instituto de Medicina Tropical Pedro Kouri, La Habana, Cuba
    Rev Cubana Med Trop 64:5-14. 2012
  3. doi request reprint Secondary infection as a risk factor for dengue hemorrhagic fever/dengue shock syndrome: an historical perspective and role of antibody-dependent enhancement of infection
    Maria G Guzman
    Institute of Tropical Medicine Pedro Kouri, Havana, Cuba
    Arch Virol 158:1445-59. 2013
  4. ncbi request reprint [Quality control of the serological diagnosis of dengue in laboratories throughout the Americas, 1996-2001]
    Maria G Guzman
    Instituto de Medicina Tropical, Departamento de Virologia, Centro Colaborador OPS OMS para el Estudio de las Enfermedades Víricas, Ciudad de La Habana, Cuba
    Rev Panam Salud Publica 14:371-6. 2003
  5. doi request reprint Dengue haemorrhagic fever integral hypothesis: confirming observations, 1987-2007
    Maria G Guzman
    Virology Department, PAHO WHO Collaborating Center for the Study of Dengue and its Vector, Institute of Tropical Medicine, Pedro Kouri, Habana, Cuba
    Trans R Soc Trop Med Hyg 102:522-3. 2008
  6. ncbi request reprint Dengue vaccine: priorities and progress
    Maria G Guzman
    Pedro Kouri Tropical Medicine Institute, Autopista Novia del Mediodia, Km 6 1 2 P O Box Marianao 13, C Habana, Cuba
    Expert Rev Anti Infect Ther 2:895-911. 2004
  7. pmc Neutralizing antibodies after infection with dengue 1 virus
    Maria G Guzman
    Pedro Kouri Tropical Medicine Institute, Havana, Cuba
    Emerg Infect Dis 13:282-6. 2007
  8. ncbi request reprint [Final characterization of and lessons learned from the dengue 3 epidemic in Cuba, 2001-2002]
    Maria G Guzman
    Centro Colaborador OPS OMS para el Estudio del Dengue y su Vector, Instituto de Medicina Tropical Pedro Kouri, Ciudad de La Habana, Cuba
    Rev Panam Salud Publica 19:282-9. 2006
  9. ncbi request reprint [Notes on the laboratory diagnosis of dengue virus]
    Mará G Guzmán
    Departmento de Virologia, Centro Colaborador de la OPS OMS para el Estudio de las Enfermedades Víricas, Instituto de Medicina Tropical Pedro Kouri, Ciudad de La Habana, Cuba
    Rev Cubana Med Trop 54:180-8. 2002
  10. ncbi request reprint [Dengue and dengue hemorrhagic fever: research priorities]
    Maria G Guzman
    Instituto de Medicina Tropical Pedro Kouri, Ciudad de La Habana, Cuba
    Rev Panam Salud Publica 19:204-15. 2006

Collaborators

Detail Information

Publications68

  1. ncbi request reprint Do escape mutants explain rapid increases in dengue case-fatality rates within epidemics?
    M G Guzman
    Institute of Tropical Medicine Pedro Kouri, Havana, Cuba
    Lancet 355:1902-3. 2000
    ..When inoculated into a new dengue-1-immune host, these viruses are free to interact with the more abundant infection-enhancing antibodies to produce severe disease...
  2. ncbi request reprint [Thirty years after the Cuban dengue hemorrhagic epidemic occurred in 1981]
    C María G Guzmán Tirado
    Instituto de Medicina Tropical Pedro Kouri, La Habana, Cuba
    Rev Cubana Med Trop 64:5-14. 2012
    ..This paper summarized some interesting aspects related with this epidemic as well as with the laboratory surveillance and results of some of the main research works...
  3. doi request reprint Secondary infection as a risk factor for dengue hemorrhagic fever/dengue shock syndrome: an historical perspective and role of antibody-dependent enhancement of infection
    Maria G Guzman
    Institute of Tropical Medicine Pedro Kouri, Havana, Cuba
    Arch Virol 158:1445-59. 2013
    ..This review is focused on the etiological role of secondary infections (SI) and mechanisms of ADE. ..
  4. ncbi request reprint [Quality control of the serological diagnosis of dengue in laboratories throughout the Americas, 1996-2001]
    Maria G Guzman
    Instituto de Medicina Tropical, Departamento de Virologia, Centro Colaborador OPS OMS para el Estudio de las Enfermedades Víricas, Ciudad de La Habana, Cuba
    Rev Panam Salud Publica 14:371-6. 2003
    ..To report the results from participating laboratories for four external quality control proficiency tests of dengue serological diagnosis that were carried out in the Region of the Americas in the period of 1996-2001...
  5. doi request reprint Dengue haemorrhagic fever integral hypothesis: confirming observations, 1987-2007
    Maria G Guzman
    Virology Department, PAHO WHO Collaborating Center for the Study of Dengue and its Vector, Institute of Tropical Medicine, Pedro Kouri, Habana, Cuba
    Trans R Soc Trop Med Hyg 102:522-3. 2008
    ..Investigations on the interaction among host, virus and mosquito with an ecosystemic view are needed...
  6. ncbi request reprint Dengue vaccine: priorities and progress
    Maria G Guzman
    Pedro Kouri Tropical Medicine Institute, Autopista Novia del Mediodia, Km 6 1 2 P O Box Marianao 13, C Habana, Cuba
    Expert Rev Anti Infect Ther 2:895-911. 2004
    ..This review will discuss the requirements of a dengue vaccine, problems, and advances that have been made. Finally, new targets for research will be presented...
  7. pmc Neutralizing antibodies after infection with dengue 1 virus
    Maria G Guzman
    Pedro Kouri Tropical Medicine Institute, Havana, Cuba
    Emerg Infect Dis 13:282-6. 2007
    ..This finding may be related to the time-dependent changes in severity of disease found with secondary dengue infection...
  8. ncbi request reprint [Final characterization of and lessons learned from the dengue 3 epidemic in Cuba, 2001-2002]
    Maria G Guzman
    Centro Colaborador OPS OMS para el Estudio del Dengue y su Vector, Instituto de Medicina Tropical Pedro Kouri, Ciudad de La Habana, Cuba
    Rev Panam Salud Publica 19:282-9. 2006
  9. ncbi request reprint [Notes on the laboratory diagnosis of dengue virus]
    Mará G Guzmán
    Departmento de Virologia, Centro Colaborador de la OPS OMS para el Estudio de las Enfermedades Víricas, Instituto de Medicina Tropical Pedro Kouri, Ciudad de La Habana, Cuba
    Rev Cubana Med Trop 54:180-8. 2002
    ..Some aspects of interests connected with the virological, serological and molecular diagnosis of dengue and its most severe form, dengue hemorrhagic fever, which has turned into the most important arthropod-borne viral disease, are updated...
  10. ncbi request reprint [Dengue and dengue hemorrhagic fever: research priorities]
    Maria G Guzman
    Instituto de Medicina Tropical Pedro Kouri, Ciudad de La Habana, Cuba
    Rev Panam Salud Publica 19:204-15. 2006
    ....
  11. ncbi request reprint Dengue diagnosis, advances and challenges
    Maria G Guzman
    Virology Department, PAHO WHO Collaborating Center for Viral Diseases, Pedro Kouri Tropical Medicine Institute, Autopista Novia del Mediodi a, Km 6, Ciudad Habana, Cuba
    Int J Infect Dis 8:69-80. 2004
    ....
  12. ncbi request reprint Global voices of science. Deciphering dengue: the Cuban experience
    Maria G Guzman
    Virology Department, PAHO WHO Collaborating Center for Viral Diseases, Instituto de Medicina Tropical Pedro Kouri Autopista Novia del Mediodía, Km 1 2, Post Office Box Mariano 13, Habana, Cuba
    Science 309:1495-7. 2005
  13. ncbi request reprint Dengue: an update
    Maria G Guzman
    Virology Department, PAHO WHO Collaborating Center for Viral Diseases, Pedro Kouri Tropical Medicine Institute, Autopista Novia del Mediodia, Km 6, PO Box Marianao 13, Ciudad Habana, Cuba
    Lancet Infect Dis 2:33-42. 2002
    ..We describe the status of vaccine development and emphasise that the only alternative that we have today to control the disease is through control of its vector Aedes aegypti...
  14. doi request reprint Epidemiological studies on dengue virus type 3 in Playa municipality, Havana, Cuba, 2001-2002
    Maria G Guzman
    Department of Virology, PAHO WHO Collaborating Center for Study of Dengue and its Vector, Pedro Kouri Tropical Medicine Institute, Autopista Novia del Mediodía Km 6, PO Box 601, Marianao 13, Havana, Cuba
    Int J Infect Dis 16:e198-203. 2012
    ....
  15. ncbi request reprint Dengue and dengue hemorrhagic fever in the Americas: lessons and challenges
    Maria G Guzman
    Virology Department, PAHO WHO Collaborating Center for Viral Diseases, Pedro Kouri Tropical Medicine Institute, Autopista Novia del Mediodia, Km 6 P O Box Marianao 13, Havana, Cuba
    J Clin Virol 27:1-13. 2003
    ..In conclusion, without urgent action for the prevention and control of dengue/DHF and its vector, the current situation will worsen and, more dramatical, there is a risk of the urbanization of yellow fever...
  16. doi request reprint Domain III of the envelope protein as a dengue vaccine target
    Maria G Guzman
    Department of Virology, PAHO WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kouri Tropical Medicine Institute, Autopista Novia del Mediodia, Km 6, Apdo 601, Marianao 13, Havana, Cuba
    Expert Rev Vaccines 9:137-47. 2010
    ..Due to the results obtained concerning the immune response and protection in mice and monkeys, particular attention is paid to the chimeric protein domain III fused to p64k of Neisseria meningitidis...
  17. ncbi request reprint Oxidative stress in adult dengue patients
    Lizette Gil
    Department of Clinical Pharmacology, Hospital Institute of Tropical Medicine Pedro Kourí, Marianao 13, Havana City, Havana, Cuba
    Am J Trop Med Hyg 71:652-7. 2004
    ..These findings suggest that the alteration in redox status could result of increased oxidative stress and it may play a role in the pathogenesis of the disease...
  18. ncbi request reprint Induction of neutralizing antibodies and partial protection from viral challenge in Macaca fascicularis immunized with recombinant dengue 4 virus envelope glycoprotein expressed in Pichia pastoris
    Maria G Guzman
    Virology Department, Pan American Health Organization World Health Organization Collaborating Center for Viral Diseases, Pedro Kouri Tropical Medicine Institute, Autopista Novia del Mediodia, Havana, Cuba
    Am J Trop Med Hyg 69:129-34. 2003
    ..pastoris can provide partial protection against viremia. However, the results were not effective enough to use it as a vaccine candidate. Further work is required to improve the quality of the immunogen...
  19. ncbi request reprint Amino acid changes in the recombinant Dengue 3 Envelope domain III determine its antigenicity and immunogenicity in mice
    Aida Zulueta
    Vaccines Division, Center for Genetic Engineering and Biotechnology, Ave 31 e 158 y 190, P O Box 6162, CP 10600, Cubanacan, Playa, Havana, Cuba
    Virus Res 121:65-73. 2006
    ..This finding has direct implications for the design of vaccines based on fragments of the Envelope protein...
  20. doi request reprint Immunogenicity and protective efficacy of a recombinant fusion protein containing the domain III of the dengue 1 envelope protein in non-human primates
    Lidice Bernardo
    Department of Virology, PAHO WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kourí Tropical Medicine Institute IPK, Havana, Cuba
    Antiviral Res 80:194-9. 2008
    ..These preliminary experiments are encouraging. Other formulations or vaccine schedules are being studied in an attempt to find regimens that enhance immunological protection...
  21. doi request reprint Anamnestic antibody response after viral challenge in monkeys immunized with dengue 2 recombinant fusion proteins
    Lidice Bernardo
    PAHO WHO Collaborating Center for Viral Diseases, Department of Virology, Pedro Kourí Tropical Medicine Institute IPK, Autopista Novia del Mediodia, km 6 1 2, PO Box 601, Marianao 13, Havana, Cuba
    Arch Virol 153:849-54. 2008
    ..The induction of an early HAI and IgM antibody response after challenge suggest a protective role against dengue virus (DENV) infection in monkeys, supporting their use as correlates of protection in vaccine studies...
  22. doi request reprint Serotype-specificity of recombinant fusion proteins containing domain III of dengue virus
    Alienys Izquierdo
    Virology Department, PAHO WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kouri Tropical Medicine Institute, Autopista Novia del Mediodia, km 6 1 2 P O Box 601 Marianao 13, Havana, Cuba
    Virus Res 138:135-8. 2008
    ..The serotype-specificity associated with these recombinant proteins in addition to the high antigenicity, immunogenicity and protecting capacity suggest their advantages as possible vaccine candidates...
  23. doi request reprint Phage-displayed antibody fragments recognizing dengue 3 and dengue 4 viruses as tools for viral serotyping in sera from infected individuals
    Sheila Cabezas
    Department of Virology, PAHO WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kouri Tropical Medicine Institute, Marianao, Havana, Cuba
    Arch Virol 154:1035-45. 2009
    ..These results could be the basis for the development of a high-throughput method for identifying dengue virus serotypes, which is crucial for the management and control of the disease...
  24. ncbi request reprint Dengue hemorrhagic Fever caused by sequential dengue 1-3 virus infections over a long time interval: Havana epidemic, 2001-2002
    Mayling Alvarez
    Department of Virology, Pan American Health Organization World Health Organization Collaborating Center for the Study of Dengue and its Vector, Pedro Kouri Tropical Medicine Institute, Havana, Cuba
    Am J Trop Med Hyg 75:1113-7. 2006
    ..However definitive categorization of a tertiary infection was not possible because of broadly reactive antibodies, which could have been raised by infections in the sequence DENV-1 then DENV-3...
  25. pmc Variation in inflammatory/regulatory cytokines in secondary, tertiary, and quaternary challenges with dengue virus
    Beatriz Sierra
    Department of Virology, Tropical Medicine Institute Pedro Kouri, Havana, Cuba
    Am J Trop Med Hyg 87:538-47. 2012
    ....
  26. doi request reprint Immunological evaluation in nonhuman primates of formulations based on the chimeric protein P64k-domain III of dengue 2 and two components of Neisseria meningitidis
    Iris Valdes
    Center for Genetic Engineering and Biotechnology CIGB, Havana, Cuba
    Vaccine 27:995-1001. 2009
    ..This is the first study in which the polysaccharide A of N. meningitidis is successfully employed as adjuvant for viral antigens...
  27. ncbi request reprint The fusion site of envelope fragments from each serotype of Dengue virus in the P64k protein, influence some parameters of the resulting chimeric constructs
    Aida Zulueta
    División de Vacunas, Centro de Ingeniería Genética y Biotecnologi a, Habana, Cuba
    Biochem Biophys Res Commun 308:619-26. 2003
    ..Regarding these results, we can affirm the suitability of the C-terminus fusion variants for further vaccine development as well as for a diagnostic system...
  28. doi request reprint Serotype specificity of recombinant fusion protein containing domain III and capsid protein of dengue virus 2
    Alienys Izquierdo
    PAHO WHO Collaborating Center for the Study of Dengue and its Vector, Department of Virology, Tropical Medicine Institute Pedro Kourí IPK, Autopista Novia del Mediodía Km 6, P O Box 601, Marianao 13, Havana, Cuba
    Antiviral Res 95:1-8. 2012
    ....
  29. doi request reprint The two component adjuvant IC31® potentiates the protective immunity induced by a dengue 2 recombinant fusion protein in mice
    Lidice Bernardo
    Department of Virology, PAHO WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kouri Tropical Medicine Institute, Autopista Novia del Mediodia, km 6 P O Box 601 Marianao 13, Havana, Cuba
    Vaccine 29:4256-63. 2011
    ..IC31® is a promising adjuvant for PD5 recombinant protein based vaccination against dengue. Future work should address the suitability of PD5/IC31® formulations in non-human primate models...
  30. doi request reprint Recombinant nucleocapsid-like particles from dengue-2 virus induce protective CD4+ and CD8+ cells against viral encephalitis in mice
    Lázaro Gil
    Vaccines Division, Center for Genetic Engineering and Biotechnology, Playa, Havana, Cuba
    Int Immunol 21:1175-83. 2009
    ..Further studies in non-human primates or humanized mice should be carried out to elucidate the usefulness of the NLPs as a potential vaccine candidate against dengue disease...
  31. doi request reprint Immunoglobulin G antibody response in children and adults with acute dengue 3 infection
    Susana Vazquez
    Pedro Kourí Tropical Medicine Institute IPK, PAHO WHO Collaborating Center for the Study of Dengue and its Vector, Havana City, Cuba
    J Virol Methods 159:6-9. 2009
    ..4%) and > or =1280 (83.9%), respectively. These results permitted the definition of a primary or secondary case of dengue virus infection in serum samples collected during the acute phase of dengue virus infection...
  32. pmc Primary and secondary infections of Macaca fascicularis monkeys with Asian and American genotypes of dengue virus 2
    Lidice Bernardo
    Department of Virology, PAHO WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kouri Tropical Medicine Institute, Autopista Novia del Mediodia, km 6 1 2, P O Box 601, Marianao 13, Havana, Cuba
    Clin Vaccine Immunol 15:439-46. 2008
    ..Despite the demonstration of the protective effect after homologous challenge, a strong anamnestic antibody response was observed...
  33. doi request reprint Association of MICA and MICB alleles with symptomatic dengue infection
    Gissel Garcia
    Department of Virology, Tropical Medicine Institute Pedro Kouri, Habana, Cuba
    Hum Immunol 72:904-7. 2011
    ..2, p = 0.0025) rather than the severe cases. Major histocompatibility class I-related gene-related natural killer cells and/or γδ and αβ T-cell activation might regulate the development of symptomatic DF and DHF...
  34. doi request reprint MCP-1 and MIP-1α expression in a model resembling early immune response to dengue
    Beatriz Sierra
    Virology Department, PAHO WHO Collaborating Center for the Study of Dengue and its Vector, Institute of Tropical Medicine Pedro Kouri, Autopista Novia del Mediodía Km 6, La Lisa, Havana City, Cuba
    Cytokine 52:175-83. 2010
    ....
  35. pmc Antibodies induced by dengue virus type 1 and 2 envelope domain III recombinant proteins in monkeys neutralize strains with different genotypes
    Lidice Bernardo
    Department of Virology, PAHO WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kouri Tropical Medicine Institute, Autopista Novia del Mediodia, km 6 1 2, P O Box 601, Marianao 13, Havana, Cuba
    Clin Vaccine Immunol 16:1829-31. 2009
    ..Here we demonstrated that dengue virus type 1 and 2 recombinant proteins induced high titers of neutralizing antibodies against different genotype strains...
  36. doi request reprint Neutralizing antibody response variation against dengue 3 strains
    Mayling Alvarez
    Department of Virology, PAHO WHO Collaborating Center for the Study of Dengue and its Vector Pedro Kourí Tropical Medicine Institute, Havana, Cuba
    J Med Virol 80:1783-9. 2008
    ..Observed results suggest that virus changed in the course of the epidemic. The implications of this finding in terms of dengue pathogenesis and vaccine development need to be considered...
  37. doi request reprint [Low-affinity Fcγ receptor IIa gene polymorphism and dengue bleeding disorder]
    Gissel Garcia
    Departamento de Virologia, Centro Colaborador para el estudio del Dengue y su Vector, Instituto de Medicina Tropical Pedro Kouri, La Habana, Cuba
    Enferm Infecc Microbiol Clin 30:621-3. 2012
    ..The pathophysiological changes that determine the severity of dengue are still not well known, therefore it is important to study the probable relationship with the host genetic...
  38. ncbi request reprint Effect of age on outcome of secondary dengue 2 infections
    Maria G Guzman
    Department of Virology, PAHO WHO Collaborating Center for Viral Diseases, Tropical Medicine Institute of Havana, Cuba
    Int J Infect Dis 6:118-24. 2002
    ..Involved were individuals, 3-40 year old, whose only lifetime dengue exposure was to DEN-1 in 1977 and DEN-2 in 1981. In this report we calculate age-specific DHF/DSS hospitalization and death rates based on secondary DEN 2 infections...
  39. ncbi request reprint PCR detection of dengue virus using dried whole blood spotted on filter paper
    Irina Prado
    Virology Department, PAHO WHO Collaborating Center for Viral Diseases, Pedro Kouri Tropical Medicine Institute, Autopista Novia del Mediodia, km 6 1 2, P O Box Marianao 13, Havana, Cuba
    J Virol Methods 125:75-81. 2005
    ..PCR results showed a 100% specificity and 93% sensitivity for dried blood samples. This storage method facilitates the transportation and analysis by nucleic acid amplification techniques even when freezing conditions are not available...
  40. pmc Antibody responses to Asian and American genotypes of dengue 2 virus in immunized mice
    Lidice Bernardo
    Virology Department, PAHO WHO Collaborating Center for Viral Diseases, Pedro Kouri Tropical Medicine Institute, Autopista Novia del Mediodia, Km 6, Havana, Cuba
    Clin Diagn Lab Immunol 12:361-2. 2005
    ..Differences in the neutralization pattern between the strains studied were observed, suggesting the presence of slight antigenic variations among them. The lack of recognition of one of the Asian genotype strains was remarkable...
  41. doi request reprint Tumor necrosis factor-alpha, transforming growth factor-β1, and interleukin-10 gene polymorphisms: implication in protection or susceptibility to dengue hemorrhagic fever
    Ana B Perez
    PAHO WHO Collaborating Center for the Study of Dengue and its Vector, Institute for Tropical Medicine Pedro Kouri, Havana, Cuba
    Hum Immunol 71:1135-40. 2010
    ....
  42. ncbi request reprint A fragment of the envelope protein from dengue-1 virus, fused in two different sites of the meningococcal P64k protein carrier, induces a functional immune response in mice
    Lisset Hermida
    Centro de Ingenieria Genetica y Biotecnologia, Habana, Cuba
    Biotechnol Appl Biochem 39:107-14. 2004
    ..These results reveal the availability of two sites within the P64k for the further insertion of DEN fragments, enabling a construct carrying two fragments from heterologous serotypes within the same molecule of this protein carrier...
  43. ncbi request reprint Ethnicity and difference in dengue virus-specific memory T cell responses in Cuban individuals
    Beatriz de la C Sierra
    Immunology Laboratory and Arbovirus Laboratory, Department of Virology, Pedro Kouri Institute of Tropical Medicine, La Lisa, Havana, Cuba
    Viral Immunol 19:662-8. 2006
    ....
  44. doi request reprint Virus role during intraepidemic increase in dengue disease severity
    Rosmari Rodriguez-Roche
    Pedro Kouri Tropical Medicine Institute, Havana, Cuba
    Vector Borne Zoonotic Dis 11:675-81. 2011
    ....
  45. ncbi request reprint Virus evolution during a severe dengue epidemic in Cuba, 1997
    Rosmari Rodriguez-Roche
    Department of Virology, PAHO WHO Collaborating Center for Viral Diseases, Pedro Kouri Tropical Medicine Institute, Autopista Novia del Mediodia, Km 6, PO Box 601, Marianao 13, Havana, Cuba
    Virology 334:154-9. 2005
    ..Further studies are therefore required to define the functional role of amino acid replacements observed and their possible relation to disease severity...
  46. ncbi request reprint Classical dengue hemorrhagic fever resulting from two dengue infections spaced 20 years or more apart: Havana, Dengue 3 epidemic, 2001-2002
    Daniel Gonzalez
    Pedro Kouri Tropical Medicine Institute, Virology Department, Autopista Novia del Mediodia, La Lisa, Havana City, Cuba
    Int J Infect Dis 9:280-5. 2005
    ..To report the clinical, laboratory and sonographic findings in 76 adult cases of dengue hemorrhagic fever (DHF)...
  47. pmc Diagnosis of dengue virus infection by the visual and simple AuBioDOT immunoglobulin M capture system
    Susana Vazquez
    Pedro Kouri Institute, PAHO WHO Collaborating Center for Viral Diseases, Havana City, Cuba
    Clin Diagn Lab Immunol 10:1074-7. 2003
    ..The system is useful under field conditions and in laboratories and requires little equipment...
  48. ncbi request reprint MAC-ELISA and ELISA inhibition methods for detection of antibodies after yellow fever vaccination
    Susana Vazquez
    Pedro Kouri Institute IPK, PAHO WHO Collaborating Center for Viral Diseases, Havana, Cuba
    J Virol Methods 110:179-84. 2003
    ..They are relatively rapid, simple, and they do not require sophisticated equipment. Both MAC-ELISA and ELISA inhibition methods for yellow fever could be useful for diagnosis, surveillance and yellow fever vaccine evaluation...
  49. doi request reprint Secondary heterologous dengue infection risk: Disequilibrium between immune regulation and inflammation?
    Beatriz Sierra
    Cellular Immunology Lab, Virology Department, PAHO WHO Collaborating Center for the Study of Dengue and its Vector, Institute of Tropical Medicine, Pedro Kouri Autopista Novia del Mediodia, La Lisa, Ciudad Habana, Cuba
    Cell Immunol 262:134-40. 2010
    ..pro-inflammatory pattern (IFN-gamma, TNF-alpha) in heterologous dengue virus re-challenge were found, suggesting a possible association with the higher incidence of severe dengue cases in the latter case...
  50. pmc Asymptomatic dengue infection in a Cuban population confirms the protective role of the RR variant of the FcgammaRIIa polymorphism
    Gissel Garcia
    Department of Virology, Tropical Medicine Institute Pedro Kouri, Havana, Cuba
    Am J Trop Med Hyg 82:1153-6. 2010
    ..016; odds ratio = 4.425; 95% confidence interval = 1.10-20.52) or DHF (P = 0.00018; odds ratio = 10.56; 95% confidence interval = 2.33-54.64) with respect to the subclinical infection...
  51. ncbi request reprint Enhanced severity of secondary dengue-2 infections: death rates in 1981 and 1997 Cuban outbreaks
    Maria G Guzman
    Institute of Tropical Medicine Pedro Kouri, Department of Virology, Tropical Medicine Institute, Autopista Novia del Mediodía Km 6, P O Box 601, Marianao 13, Havana, Cuba
    Rev Panam Salud Publica 11:223-7. 2002
    ..To understand the possible effect that length of time has on disease severity with sequential dengue infections...
  52. ncbi request reprint Selection of phage-displayed human antibody fragments on Dengue virus particles captured by a monoclonal antibody: application to the four serotypes
    Sheila Cabezas
    Department of Virology, PAHO WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kouri Tropical Medicine Institute, Autopista Novia del Mediodia, km 6 5, Marianao 13, Habana, Cuba
    J Virol Methods 147:235-43. 2008
    ..The simple phage selection method on captured virus could be applied in a high throughput way to obtain larger panels of antibody fragments to Dengue virus for multiple applications...
  53. ncbi request reprint Evaluation of immunoglobulin M and G capture enzyme-linked immunosorbent assay Panbio kits for diagnostic dengue infections
    Susana Vazquez
    Pedro Kouri Tropical Medicine Institute, PAHO WHO Collaborating Center for the Study of Dengue and its Vector, Havana City, Cuba
    J Clin Virol 39:194-8. 2007
    ..Serological assays are widely used to confirm dengue virus infections and to differentiate between a primary and a secondary infection...
  54. ncbi request reprint IL-10 levels in Dengue patients: some findings from the exceptional epidemiological conditions in Cuba
    Ana B Perez
    Virology Department, Pedro Kouri Tropical Medicine Institute, Ciudad de La Habana, Cuba
    J Med Virol 73:230-4. 2004
    ..The association of increased levels of IL-10 in Dengue patients with a sequential infection suggests a possible role of this cytokine in the pathogenesis of Dengue disease...
  55. pmc Dengue 3 epidemic, Havana, 2001
    Otto Peláez
    Centro Provincial de Higiene y Epidemiología de Ciudad Habana, Habana, Cuba
    Emerg Infect Dis 10:719-22. 2004
    ..Dengue 3, the etiologic agent of the epidemic, caused the dengue hemorrhagic fever only in adults, with 78 cases and 3 deaths. After intensive vector control efforts, no new cases have been detected...
  56. ncbi request reprint HLA-A, -B, -C, and -DRB1 allele frequencies in Cuban individuals with antecedents of dengue 2 disease: advantages of the Cuban population for HLA studies of dengue virus infection
    Beatriz Sierra
    Institute of Tropical Medicine Pedro Kouri, WHO Collaborator Centre for Viral Diseases, Havana, Cuba
    Hum Immunol 68:531-40. 2007
    ....
  57. doi request reprint Long-term persistence of clinical symptoms in dengue-infected persons and its association with immunological disorders
    Gissel Garcia
    Tropical Medicine Institute Pedro Kouri, Autopista Novia del Mediodia, Km 6, entre Autopista Nacional y Carretera Central, La Lisa, Apartado 601, Marianao 13, C Havana, Cuba
    Int J Infect Dis 15:e38-43. 2011
    ..This study was undertaken to determine the prevalence of persistent clinical symptoms among individuals infected during the 2006 Cuban epidemic and to evaluate the immunological and genetic factors associated with their occurrence...
  58. ncbi request reprint Long-term memory cellular immune response to dengue virus after a natural primary infection
    Beatriz Sierra
    Department of Virology, PAHO WHO Collaborating Center for Viral Diseases, Tropical Medicine Institute, Havana, Cuba
    Int J Infect Dis 6:125-8. 2002
    ..Thereafter, no dengue virus circulation was subsequently observed, except for the Santiago de Cuba municipality...
  59. doi request reprint Role of CC chemokine receptor 1 and two of its ligands in human dengue infection. Three approaches under the Cuban situation
    Beatriz Sierra
    Virology Department, PAHO WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kouri Institute of Tropical Medicine, Autopista Novia del Mediodia, Km 6, La Lisa, Habana, Cuba Electronic address
    Microbes Infect 16:40-50. 2014
    ....
  60. ncbi request reprint Apoptosis in tissues from fatal dengue shock syndrome
    Daniel Limonta
    Virology Department, PAHO WHO Collaborating Center for the Study of Dengue and its Vector, Havana, Cuba
    J Clin Virol 40:50-4. 2007
    ..Apoptosis, or programmed cell death, has been implicated in dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) pathogenesis...
  61. ncbi request reprint [Detection of lymphoproliferative response in monkeys innoculated with dengue 4 virus]
    Gissel Garcia
    Instituto de Medicina Tropical Pedro Kouni, Autopista Novia del Mediodia, km 6 1 2 La Lisa, Apartado Postal 601 Marianao 13, Ciudad de La Habana, Cuba
    Rev Cubana Med Trop 55:27-9. 2003
    ..These results showed a behavior of primates similar in some extent to that of human beings and mice regarding the specific serotype and cross serotype response to dengue virus...
  62. doi request reprint Population structure of the dengue viruses, Aragua, Venezuela, 2006-2007. Insights into dengue evolution under hyperendemic transmission
    Rosmari Rodriguez-Roche
    Pedro Kouri Tropical Medicine Institute, PO Box 601, Marianao 13, Havana, Cuba
    Infect Genet Evol 12:332-44. 2012
    ....
  63. ncbi request reprint Dual infection with dengue virus 3 and human immunodeficiency virus 1 in Havana, Cuba
    Daniel Gonzalez
    Department of Medicine, Pedro Kouri Tropical Medicine Institute, Havana, Cuba
    J Infect Dev Ctries 3:318-20. 2009
    ..To our knowledge, DEN cases caused by DEN-3 in HIV-infected individuals have not been reported previously. Further research is needed to diagnose this likely underreported mixed viral infection in DEN endemic areas...
  64. ncbi request reprint Prevalence of febrile syndromes in dengue surveillance, havana city, 2007
    Otto Peláez
    Pedro Kouri Tropical Medicine Institute, PAHO WHO Collaborating Center for the Study and Control of Dengue, Havana, Cuba
    MEDICC Rev 13:47-51. 2011
    ..Determine point prevalence of febrile syndromes and compare with prevalence reported by habitual clinical and seroepidemiologic dengue surveillance system in Havana City...
  65. doi request reprint Dengue: a continuing global threat
    Maria G Guzman
    Instituto de Medicina Tropical, Pedro Kouri, PO Box 601, Marianao 13, Ciucad de la Habana, Cuba
    Nat Rev Microbiol 8:S7-16. 2010
    ..Antiviral drugs and vaccines that are currently under development could also make an important contribution to dengue control in the future...
  66. doi request reprint Dual infection with hepatitis A and E viruses in outbreaks and in sporadic clinical cases: Cuba 1998-2003
    Licel de los Angeles Rodriguez Lay
    Institute for Tropical Medicine Pedro Kourí IPK, Havana, Cuba
    J Med Virol 80:798-802. 2008
    ..The results demonstrate the co-circulation of and co-infection with two enterically transmitted viruses; however a higher positivity was observed for anti-HAV than to anti-HEV (53.1% vs. 7.8%) in outbreaks...
  67. doi request reprint A myocarditis outbreak with fatal cases associated with adenovirus subgenera C among children from Havana City in 2005
    Clara Savón
    Tropical Medicine Institute Pedro Kouri, Havana, Cuba
    J Clin Virol 43:152-7. 2008
    ..Eleven patients had a rapid evolution of disease and there were 8 fatalities from cardiac failure secondary to myocarditis...
  68. ncbi request reprint [Economic impact of dengue 2 epidemic in Santiago de Cuba, 1997]
    Luis G Valdés
    Centro Provincial de Higiene y Epidemiología de Santiago de Cuba, Instituto de Medicina Tropical Pedro Kouri, Ciudad de La Habana
    Rev Cubana Med Trop 54:220-7. 2002