Jorge Fraga

Summary

Affiliation: Ciudad de La Habana
Country: Cuba

Publications

  1. ncbi Mycoplasma hominis in Cuban Trichomonas vaginalis isolates: association with parasite genetic polymorphism
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodía Km 6, Marianao 13, La Habana, Cuba
    Exp Parasitol 131:393-8. 2012
  2. ncbi Species typing of Cuban Trichomonas vaginalis virus by RT-PCR, and association of TVV-2 with high parasite adhesion levels and high pathogenicity in patients
    Jorge Fraga
    Laboratorio de Biologia Molecular, Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodia km 61 2, Apartado Postal 601, Marianao 13, Ciudad de La Habana, Cuba
    Arch Virol 157:1789-95. 2012
  3. ncbi Optimization of random amplified polymorphic DNA techniques for its use in genetic studies of Trichomonas vaginalis isolates
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodia km 61 2, Apartado Postal 601, Marianao 13, Ciudad de La Havana, Cuba
    Infect Genet Evol 2:73-5. 2002
  4. ncbi Double-stranded RNA viral infection in Cuban Trichomonas vaginalis isolates
    Jorge Fraga
    Department of Parasitology, Institute of Tropical Medicine, Havana, Cuba
    Braz J Infect Dis 9:521-4. 2005
  5. ncbi Optimization of random amplified polymorphic DNA techniques for use in genetic studies of Cuban Triatominae
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Ciudad de La Habana, Cuba
    Rev Inst Med Trop Sao Paulo 47:295-300. 2005
  6. ncbi Double-stranded RNA viral infection of Trichomonas vaginalis and correlation with genetic polymorphism of isolates
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Apartado Postal 601, Marianao 13, Ciudad de La Habana, Cuba
    Exp Parasitol 127:593-9. 2011
  7. ncbi Genetic variability of Triatoma flavida and Triatoma bruneri (Hemiptera: Reduviidae) by RAPD-PCR technique
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodia km 61 2, Apartado Postal 601, Marianao 13, Ciudad de La Habana, Cuba
    Rev Inst Med Trop Sao Paulo 53:19-24. 2011
  8. ncbi Genetic characterization of three Cuban Trichomonas vaginalis virus. Phylogeny of Totiviridae family
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodia km 61 2, Apartado Postal 601, Marianao 13, Ciudad de La Habana, Cuba
    Infect Genet Evol 12:113-20. 2012
  9. ncbi Molecular diagnosis of Toxoplasma gondii infection in cerebrospinal fluid from AIDS patients
    Yenisey Alfonso
    Parasitology Department, Institute of Tropical Medicine Pedro Kouri, PO Box 601, Marianao 13, Ciudad de La Habana, Cuba
    Cerebrospinal Fluid Res 6:2. 2009
  10. ncbi Detection of Toxoplasma gondii in cerebrospinal fluid from AIDS patients by nested PCR and rapid identification of type I allele at B1 gene by RFLP analysis
    Yenisey Alfonso
    Parasitology Department, Molecular Biology Laboratory, Institute of Tropical Medicine Pedro Kouri, Marianao 13, Ciudad de La Habana, Cuba
    Exp Parasitol 122:203-7. 2009

Collaborators

Detail Information

Publications23

  1. ncbi Mycoplasma hominis in Cuban Trichomonas vaginalis isolates: association with parasite genetic polymorphism
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodía Km 6, Marianao 13, La Habana, Cuba
    Exp Parasitol 131:393-8. 2012
    ..hominis in T. vaginalis isolates. This result could point to a predisposition of T. vaginalis for the bacterial enters and/or survival...
  2. ncbi Species typing of Cuban Trichomonas vaginalis virus by RT-PCR, and association of TVV-2 with high parasite adhesion levels and high pathogenicity in patients
    Jorge Fraga
    Laboratorio de Biologia Molecular, Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodia km 61 2, Apartado Postal 601, Marianao 13, Ciudad de La Habana, Cuba
    Arch Virol 157:1789-95. 2012
    ..According to our results, it seems that only two TVV species are infecting the Cuban isolates. Further studies using higher number of strains should be conducted in order to corroborate these results...
  3. ncbi Optimization of random amplified polymorphic DNA techniques for its use in genetic studies of Trichomonas vaginalis isolates
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodia km 61 2, Apartado Postal 601, Marianao 13, Ciudad de La Havana, Cuba
    Infect Genet Evol 2:73-5. 2002
    ....
  4. ncbi Double-stranded RNA viral infection in Cuban Trichomonas vaginalis isolates
    Jorge Fraga
    Department of Parasitology, Institute of Tropical Medicine, Havana, Cuba
    Braz J Infect Dis 9:521-4. 2005
    ..vaginalis isolates. This gives an estimate of the infection rate of Cuban T. vaginalis isolates by the dsRNA virus. Future research should focus on the association between trichomonosis symptoms and the presence of TVV...
  5. ncbi Optimization of random amplified polymorphic DNA techniques for use in genetic studies of Cuban Triatominae
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Ciudad de La Habana, Cuba
    Rev Inst Med Trop Sao Paulo 47:295-300. 2005
    ..T. flavida present low interpopulation variability that could result in greater susceptibility to pesticides in control programs. The RAPD protocol and the selected primers are useful for molecular characterization of Cuban Triatominae...
  6. ncbi Double-stranded RNA viral infection of Trichomonas vaginalis and correlation with genetic polymorphism of isolates
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Apartado Postal 601, Marianao 13, Ciudad de La Habana, Cuba
    Exp Parasitol 127:593-9. 2011
    ..028) demonstrating the existence of concordance between the genetic relatedness and the presence of TVV in T. vaginalis isolates. This result could point to a predisposition of T. vaginalis for the viral enters and/or survival...
  7. ncbi Genetic variability of Triatoma flavida and Triatoma bruneri (Hemiptera: Reduviidae) by RAPD-PCR technique
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodia km 61 2, Apartado Postal 601, Marianao 13, Ciudad de La Habana, Cuba
    Rev Inst Med Trop Sao Paulo 53:19-24. 2011
    ..Our results confirm the genetic variability among T. flavida and T. bruneri species...
  8. ncbi Genetic characterization of three Cuban Trichomonas vaginalis virus. Phylogeny of Totiviridae family
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodia km 61 2, Apartado Postal 601, Marianao 13, Ciudad de La Habana, Cuba
    Infect Genet Evol 12:113-20. 2012
    ....
  9. ncbi Molecular diagnosis of Toxoplasma gondii infection in cerebrospinal fluid from AIDS patients
    Yenisey Alfonso
    Parasitology Department, Institute of Tropical Medicine Pedro Kouri, PO Box 601, Marianao 13, Ciudad de La Habana, Cuba
    Cerebrospinal Fluid Res 6:2. 2009
    ..The aim of the present study was to evaluate a rapid PCR method using the B1 gene to detect T. gondii in cerebrospinal fluid (CSF) samples from patients with suspected TE...
  10. ncbi Detection of Toxoplasma gondii in cerebrospinal fluid from AIDS patients by nested PCR and rapid identification of type I allele at B1 gene by RFLP analysis
    Yenisey Alfonso
    Parasitology Department, Molecular Biology Laboratory, Institute of Tropical Medicine Pedro Kouri, Marianao 13, Ciudad de La Habana, Cuba
    Exp Parasitol 122:203-7. 2009
    ..This is the first report of evaluation of S1-AS1/S2-AS2 set of primers in more than 100 clinical samples as well as the first genotyping study of T. gondii in Cuba...
  11. ncbi Conventional polymerase chain reaction for the diagnosis of neurotoxoplasmosis: comparison of three sets of primers for the B1 gene using CSF samples
    Yenisey Alfonso
    Parasitology Department, Institute of Tropical Medicine Pedro Kouri, Havana, Cuba
    Diagn Microbiol Infect Dis 75:150-4. 2013
    ....
  12. ncbi Genetic variability between Trichomonas vaginalis isolates and correlation with clinical presentation
    Lazara Rojas
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kourí Autopista Novia del Mediodía Km 6 1 2, Apartado Postal 601, Marianao 13, Ciudad de La Habana, Cuba
    Infect Genet Evol 4:53-8. 2004
    ..This is the first description of a possible virulence marker for T. vaginalis. Further studies will be necessary to ascertain the importance and function of this genetic marker in clinical infection...
  13. ncbi Comparison of four DNA extraction methods from cerebrospinal fluid for the detection of Toxoplasma gondii by polymerase chain reaction in AIDS patients
    Yenisey Alfonso
    Department of Parasitology, Institute of Tropical Medicine Pedro Kouri, Ciudad de La Habana, Cuba
    Med Sci Monit 14:MT1-6. 2008
    ..The aim of the present study was to compare four DNA extraction methods for the detection of T. gondii on cerebrospinal fluid (CSF) using the PCR technology...
  14. ncbi Phylogeny of Leishmania species based on the heat-shock protein 70 gene
    Jorge Fraga
    Parasitology Department, Institute of Tropical Medicine Pedro Kouri, La Havana, Cuba
    Infect Genet Evol 10:238-45. 2010
    ..L.) amazonensis did not form monophyletic clusters. These findings are discussed in relation to results from other genes and proteins, which have to be integrated in order to build a genetically supported taxonomy for the entire genus...
  15. ncbi Use of in vitro cytoadherence assays in the comparative study of the virulence of isolates of Trichomonas vaginalis
    Lazara Rojas
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodia km 61 2, Apartado Postal 601, Marianao 13 Ciudad de La Habana, Cuba
    Parasitol Res 93:332-7. 2004
    ..A correlation coefficient of 0.97 was found between the pattern curves obtained by both labeling procedures. The level of adhesion to HeLa cells was directly proportional to the severity of the clinical manifestations (P < 0.05)...
  16. ncbi Detection and genetic distance of resistant populations of Pseudosuccinea columella (Mollusca: Lymnaeidae) to Fasciola hepatica (Trematoda: Digenea) using RAPD markers
    Aymé Fernandez Calienes
    Laboratorio de Malacologia, Instituto Pedro Kouri, IPK Apartado 601, Marianao 13, Ciudad Habana, Cuba
    Acta Trop 92:83-7. 2004
    ..hepatica. However, clear differences in RAPD banding patterns and genetic distance were observed between resistant and susceptible isolates...
  17. ncbi Accurate and rapid species typing from cutaneous and mucocutaneous leishmaniasis lesions of the New World
    Jorge Fraga
    Institute of Tropical Medicine Pedro Kouri, 13600 Havana, Cuba
    Diagn Microbiol Infect Dis 74:142-50. 2012
    ..This study is a paradigm for the implementation of a globally applicable upgraded tool for the identification of Leishmania directly on human specimens from cutaneous and mucocutaneous lesions in the New World...
  18. ncbi [Leishmaniasis diagnosis: going from microscopic observation of parasite to DNA detection]
    Ana M Montalvo
    Instituto de Medicina Tropical Pedro Kouri, La Habana, Cuba
    Rev Cubana Med Trop 64:108-31. 2012
    ..All the aforementioned has an impact on the diagnostic method that should be used...
  19. ncbi [Amplification at random of the DNA from 5 Cuban populations of larviphage fishes of the Rivulus genus]
    Aymé Fernández-Calienes
    Instituto de Medicina Tropical Pedro Kouri, Ciudad de La Habana, Cuba
    Rev Cubana Med Trop 55:203-7. 2003
    ..These results support the use of RAPD as an efficient tool for the genetic characterization of Cuban fishes of the Rivulus genus...
  20. ncbi [PCR-RFLP and RAPD for typing neotropical Leishmania]
    Ana Margarita Montalvo
    Departamento de Parasitologia, Instituto Pedro Kouri, La Habana, Cuba
    Biomedica 28:597-606. 2008
    ..The analysis of the PCR-restriction fragment length polymorphism and random amplified polymorphic DNA have been useful tools for Leishmania identification...
  21. ncbi Genetic characterization by random amplified polymorphic DNA analysis (RAPD) of 18 isolates of Giardia lamblia obtained from day care children
    Liliana Pelayo
    Department of Parasitology, Tropical Medicine Institute Pedro Kour, , Central, Apartado Postal 601, CP 11300, Ciudad de La Habana, Cuba
    Exp Parasitol 104:162-6. 2003
  22. ncbi In vitro antimicrobial assessment of Cuban propolis extracts
    Lianet Monzote
    Department of Parasitology, Institute of Tropical Medicine Pedro Kouri, Havana, Cuba
    Mem Inst Oswaldo Cruz 107:978-84. 2012
    ..The observed chemical composition-activity relationship of propolis can contribute to the identification of the active principles and standardisation of this bee product...
  23. ncbi Fasciola hepatica: identification of molecular markers for resistant and susceptible Pseudosuccinea columella snail hosts
    Alfredo Gutierrez
    Laboratorio de Malacologia, Instituto Pedro Kouri, IPK Apartado 601, Marianao 13, Ciudad Habana, Cuba
    Exp Parasitol 105:211-8. 2003
    ..This is the first time that a genetic variation using RAPD markers is demonstrated between susceptible and resistant lymnaeid snails vis-a-vis of F. hepatica infection in absence of experimental selection...