Jorge Fraga

Summary

Affiliation: Ciudad de La Habana
Country: Cuba

Publications

  1. doi request reprint Mycoplasma hominis in Cuban Trichomonas vaginalis isolates: association with parasite genetic polymorphism
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodía Km 6, Marianao 13, La Habana, Cuba
    Exp Parasitol 131:393-8. 2012
  2. doi request reprint Species typing of Cuban Trichomonas vaginalis virus by RT-PCR, and association of TVV-2 with high parasite adhesion levels and high pathogenicity in patients
    Jorge Fraga
    Laboratorio de Biologia Molecular, Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodia km 61 2, Apartado Postal 601, Marianao 13, Ciudad de La Habana, Cuba
    Arch Virol 157:1789-95. 2012
  3. ncbi request reprint Optimization of random amplified polymorphic DNA techniques for its use in genetic studies of Trichomonas vaginalis isolates
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodia km 61 2, Apartado Postal 601, Marianao 13, Ciudad de La Havana, Cuba
    Infect Genet Evol 2:73-5. 2002
  4. pmc Molecular diagnosis of Toxoplasma gondii infection in cerebrospinal fluid from AIDS patients
    Yenisey Alfonso
    Parasitology Department, Institute of Tropical Medicine Pedro Kouri, PO Box 601, Marianao 13, Ciudad de La Habana, Cuba
    Cerebrospinal Fluid Res 6:2. 2009
  5. doi request reprint Double-stranded RNA viral infection of Trichomonas vaginalis and correlation with genetic polymorphism of isolates
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Apartado Postal 601, Marianao 13, Ciudad de La Habana, Cuba
    Exp Parasitol 127:593-9. 2011
  6. ncbi request reprint Double-stranded RNA viral infection in Cuban Trichomonas vaginalis isolates
    Jorge Fraga
    Department of Parasitology, Institute of Tropical Medicine, Havana, Cuba
    Braz J Infect Dis 9:521-4. 2005
  7. ncbi request reprint Optimization of random amplified polymorphic DNA techniques for use in genetic studies of Cuban Triatominae
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Ciudad de La Habana, Cuba
    Rev Inst Med Trop Sao Paulo 47:295-300. 2005
  8. ncbi request reprint Genetic variability of Triatoma flavida and Triatoma bruneri (Hemiptera: Reduviidae) by RAPD-PCR technique
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodia km 61 2, Apartado Postal 601, Marianao 13, Ciudad de La Habana, Cuba
    Rev Inst Med Trop Sao Paulo 53:19-24. 2011
  9. doi request reprint Genetic characterization of three Cuban Trichomonas vaginalis virus. Phylogeny of Totiviridae family
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodia km 61 2, Apartado Postal 601, Marianao 13, Ciudad de La Habana, Cuba
    Infect Genet Evol 12:113-20. 2012
  10. doi request reprint Detection of Toxoplasma gondii in cerebrospinal fluid from AIDS patients by nested PCR and rapid identification of type I allele at B1 gene by RFLP analysis
    Yenisey Alfonso
    Parasitology Department, Molecular Biology Laboratory, Institute of Tropical Medicine Pedro Kouri, Marianao 13, Ciudad de La Habana, Cuba
    Exp Parasitol 122:203-7. 2009

Collaborators

Detail Information

Publications27

  1. doi request reprint Mycoplasma hominis in Cuban Trichomonas vaginalis isolates: association with parasite genetic polymorphism
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodía Km 6, Marianao 13, La Habana, Cuba
    Exp Parasitol 131:393-8. 2012
    ..hominis in T. vaginalis isolates. This result could point to a predisposition of T. vaginalis for the bacterial enters and/or survival...
  2. doi request reprint Species typing of Cuban Trichomonas vaginalis virus by RT-PCR, and association of TVV-2 with high parasite adhesion levels and high pathogenicity in patients
    Jorge Fraga
    Laboratorio de Biologia Molecular, Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodia km 61 2, Apartado Postal 601, Marianao 13, Ciudad de La Habana, Cuba
    Arch Virol 157:1789-95. 2012
    ..According to our results, it seems that only two TVV species are infecting the Cuban isolates. Further studies using higher number of strains should be conducted in order to corroborate these results...
  3. ncbi request reprint Optimization of random amplified polymorphic DNA techniques for its use in genetic studies of Trichomonas vaginalis isolates
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodia km 61 2, Apartado Postal 601, Marianao 13, Ciudad de La Havana, Cuba
    Infect Genet Evol 2:73-5. 2002
    ....
  4. pmc Molecular diagnosis of Toxoplasma gondii infection in cerebrospinal fluid from AIDS patients
    Yenisey Alfonso
    Parasitology Department, Institute of Tropical Medicine Pedro Kouri, PO Box 601, Marianao 13, Ciudad de La Habana, Cuba
    Cerebrospinal Fluid Res 6:2. 2009
    ..The aim of the present study was to evaluate a rapid PCR method using the B1 gene to detect T. gondii in cerebrospinal fluid (CSF) samples from patients with suspected TE...
  5. doi request reprint Double-stranded RNA viral infection of Trichomonas vaginalis and correlation with genetic polymorphism of isolates
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Apartado Postal 601, Marianao 13, Ciudad de La Habana, Cuba
    Exp Parasitol 127:593-9. 2011
    ..028) demonstrating the existence of concordance between the genetic relatedness and the presence of TVV in T. vaginalis isolates. This result could point to a predisposition of T. vaginalis for the viral enters and/or survival...
  6. ncbi request reprint Double-stranded RNA viral infection in Cuban Trichomonas vaginalis isolates
    Jorge Fraga
    Department of Parasitology, Institute of Tropical Medicine, Havana, Cuba
    Braz J Infect Dis 9:521-4. 2005
    ..vaginalis isolates. This gives an estimate of the infection rate of Cuban T. vaginalis isolates by the dsRNA virus. Future research should focus on the association between trichomonosis symptoms and the presence of TVV...
  7. ncbi request reprint Optimization of random amplified polymorphic DNA techniques for use in genetic studies of Cuban Triatominae
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Ciudad de La Habana, Cuba
    Rev Inst Med Trop Sao Paulo 47:295-300. 2005
    ..T. flavida present low interpopulation variability that could result in greater susceptibility to pesticides in control programs. The RAPD protocol and the selected primers are useful for molecular characterization of Cuban Triatominae...
  8. ncbi request reprint Genetic variability of Triatoma flavida and Triatoma bruneri (Hemiptera: Reduviidae) by RAPD-PCR technique
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodia km 61 2, Apartado Postal 601, Marianao 13, Ciudad de La Habana, Cuba
    Rev Inst Med Trop Sao Paulo 53:19-24. 2011
    ..Our results confirm the genetic variability among T. flavida and T. bruneri species...
  9. doi request reprint Genetic characterization of three Cuban Trichomonas vaginalis virus. Phylogeny of Totiviridae family
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodia km 61 2, Apartado Postal 601, Marianao 13, Ciudad de La Habana, Cuba
    Infect Genet Evol 12:113-20. 2012
    ....
  10. doi request reprint Detection of Toxoplasma gondii in cerebrospinal fluid from AIDS patients by nested PCR and rapid identification of type I allele at B1 gene by RFLP analysis
    Yenisey Alfonso
    Parasitology Department, Molecular Biology Laboratory, Institute of Tropical Medicine Pedro Kouri, Marianao 13, Ciudad de La Habana, Cuba
    Exp Parasitol 122:203-7. 2009
    ..This is the first report of evaluation of S1-AS1/S2-AS2 set of primers in more than 100 clinical samples as well as the first genotyping study of T. gondii in Cuba...
  11. doi request reprint Conventional polymerase chain reaction for the diagnosis of neurotoxoplasmosis: comparison of three sets of primers for the B1 gene using CSF samples
    Yenisey Alfonso
    Parasitology Department, Institute of Tropical Medicine Pedro Kouri, Havana, Cuba
    Diagn Microbiol Infect Dis 75:150-4. 2013
    ....
  12. ncbi request reprint Genetic variability between Trichomonas vaginalis isolates and correlation with clinical presentation
    Lazara Rojas
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kourí Autopista Novia del Mediodía Km 6 1 2, Apartado Postal 601, Marianao 13, Ciudad de La Habana, Cuba
    Infect Genet Evol 4:53-8. 2004
    ..This is the first description of a possible virulence marker for T. vaginalis. Further studies will be necessary to ascertain the importance and function of this genetic marker in clinical infection...
  13. doi request reprint Phylogeny of Leishmania species based on the heat-shock protein 70 gene
    Jorge Fraga
    Parasitology Department, Institute of Tropical Medicine Pedro Kouri, La Havana, Cuba
    Infect Genet Evol 10:238-45. 2010
    ..L.) amazonensis did not form monophyletic clusters. These findings are discussed in relation to results from other genes and proteins, which have to be integrated in order to build a genetically supported taxonomy for the entire genus...
  14. ncbi request reprint Comparison of four DNA extraction methods from cerebrospinal fluid for the detection of Toxoplasma gondii by polymerase chain reaction in AIDS patients
    Yenisey Alfonso
    Department of Parasitology, Institute of Tropical Medicine Pedro Kouri, Ciudad de La Habana, Cuba
    Med Sci Monit 14:MT1-6. 2008
    ..The aim of the present study was to compare four DNA extraction methods for the detection of T. gondii on cerebrospinal fluid (CSF) using the PCR technology...
  15. ncbi request reprint Use of in vitro cytoadherence assays in the comparative study of the virulence of isolates of Trichomonas vaginalis
    Lazara Rojas
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Autopista Novia del Mediodia km 61 2, Apartado Postal 601, Marianao 13 Ciudad de La Habana, Cuba
    Parasitol Res 93:332-7. 2004
    ..A correlation coefficient of 0.97 was found between the pattern curves obtained by both labeling procedures. The level of adhesion to HeLa cells was directly proportional to the severity of the clinical manifestations (P < 0.05)...
  16. ncbi request reprint Detection and genetic distance of resistant populations of Pseudosuccinea columella (Mollusca: Lymnaeidae) to Fasciola hepatica (Trematoda: Digenea) using RAPD markers
    Aymé Fernandez Calienes
    Laboratorio de Malacologia, Instituto Pedro Kouri, IPK Apartado 601, Marianao 13, Ciudad Habana, Cuba
    Acta Trop 92:83-7. 2004
    ..hepatica. However, clear differences in RAPD banding patterns and genetic distance were observed between resistant and susceptible isolates...
  17. doi request reprint Differentiation between Trypanosoma cruzi and Trypanosoma rangeli using heat-shock protein 70 polymorphisms
    Jorge Fraga
    Parasitology Department, Institute of Tropical Medicine Pedro Kouri, La Havana, Cuba
    Trop Med Int Health 19:195-206. 2014
    ..We developed and validated a generic PCR discriminating the species by restriction fragment length polymorphism (RFLP) analysis and a duplex PCR specifically amplifying a differently sized fragment of both species...
  18. doi request reprint Accurate and rapid species typing from cutaneous and mucocutaneous leishmaniasis lesions of the New World
    Jorge Fraga
    Institute of Tropical Medicine Pedro Kouri, 13600 Havana, Cuba
    Diagn Microbiol Infect Dis 74:142-50. 2012
    ..This study is a paradigm for the implementation of a globally applicable upgraded tool for the identification of Leishmania directly on human specimens from cutaneous and mucocutaneous lesions in the New World...
  19. ncbi request reprint [Leishmaniasis diagnosis: going from microscopic observation of parasite to DNA detection]
    Ana M Montalvo
    Instituto de Medicina Tropical Pedro Kouri, La Habana, Cuba
    Rev Cubana Med Trop 64:108-31. 2012
    ..All the aforementioned has an impact on the diagnostic method that should be used...
  20. doi request reprint HindII and SduI digests of heat-shock protein 70 PCR for Leishmania typing
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Havana, Cuba
    Diagn Microbiol Infect Dis 77:245-7. 2013
    ..braziliensis group, which was previously not possible. ..
  21. ncbi request reprint [Amplification at random of the DNA from 5 Cuban populations of larviphage fishes of the Rivulus genus]
    Aymé Fernández-Calienes
    Instituto de Medicina Tropical Pedro Kouri, Ciudad de La Habana, Cuba
    Rev Cubana Med Trop 55:203-7. 2003
    ..These results support the use of RAPD as an efficient tool for the genetic characterization of Cuban fishes of the Rivulus genus...
  22. doi request reprint Evolution and species discrimination according to the Leishmania heat-shock protein 20 gene
    Jorge Fraga
    Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kouri, Habana, Cuba
    Infect Genet Evol 18:229-37. 2013
    ..In addition, this study represents a solid contribution to the objective of establishing a more reliable taxonomy for the genus Leishmania. ..
  23. ncbi request reprint [PCR-RFLP and RAPD for typing neotropical Leishmania]
    Ana Margarita Montalvo
    Departamento de Parasitologia, Instituto Pedro Kouri, La Habana, Cuba
    Biomedica 28:597-606. 2008
    ..The analysis of the PCR-restriction fragment length polymorphism and random amplified polymorphic DNA have been useful tools for Leishmania identification...
  24. ncbi request reprint [Microbiological characterization of non-O1 Vibrio cholerae isolated in Cuba]
    Laura Bravo Fariñas
    Instituto de Medicina Tropical Pedro Kouri, Ciudad de La Habana, Cuba
    Rev Cubana Med Trop 59:227-33. 2007
    ..The results of the molecular and epidemiological studies allowed giving a speedy and accurate response that explained the etiology of the first food-borne disease outbreak...
  25. ncbi request reprint In vitro antimicrobial assessment of Cuban propolis extracts
    Lianet Monzote
    Department of Parasitology, Institute of Tropical Medicine Pedro Kouri, Havana, Cuba
    Mem Inst Oswaldo Cruz 107:978-84. 2012
    ..The observed chemical composition-activity relationship of propolis can contribute to the identification of the active principles and standardisation of this bee product...
  26. ncbi request reprint Genetic characterization by random amplified polymorphic DNA analysis (RAPD) of 18 isolates of Giardia lamblia obtained from day care children
    Liliana Pelayo
    Department of Parasitology, Tropical Medicine Institute Pedro Kouri, Autopista Novia del Mediodía Km 6 e Autopista Nacional y Carretera, Central, Apartado Postal 601, CP 11300, Ciudad de La Habana, Cuba
    Exp Parasitol 104:162-6. 2003
  27. ncbi request reprint Fasciola hepatica: identification of molecular markers for resistant and susceptible Pseudosuccinea columella snail hosts
    Alfredo Gutierrez
    Laboratorio de Malacologia, Instituto Pedro Kouri, IPK Apartado 601, Marianao 13, Ciudad Habana, Cuba
    Exp Parasitol 105:211-8. 2003
    ..This is the first time that a genetic variation using RAPD markers is demonstrated between susceptible and resistant lymnaeid snails vis-a-vis of F. hepatica infection in absence of experimental selection...