John R Couchman

Summary

Publications

  1. doi request reprint Transmembrane signaling proteoglycans
    John R Couchman
    Department of Biomedical Sciences, University of Copenhagen, Copenhagen, 2200 Denmark
    Annu Rev Cell Dev Biol 26:89-114. 2010
  2. pmc Heparan sulfate chain valency controls syndecan-4 function in cell adhesion
    Sandeep Gopal
    Department of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark
    J Biol Chem 285:14247-58. 2010
  3. pmc Serine 34 phosphorylation of rho guanine dissociation inhibitor (RhoGDIalpha) links signaling from conventional protein kinase C to RhoGTPase in cell adhesion
    Athanassios Dovas
    Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, Exhibition Road, London SW7 2AZ, United Kingdom
    J Biol Chem 285:23296-308. 2010
  4. pmc An introduction to proteoglycans and their localization
    John R Couchman
    Department of Biomedical Sciences, University of Copenhagen, Copenhagen N, Denmark
    J Histochem Cytochem 60:885-97. 2012
  5. ncbi request reprint Hierarchy of ADAM12 binding to integrins in tumor cells
    Charles Kumar Thodeti
    Institute of Molecular Pathology, University of Copenhagen, Frederik V s vej 11, DK 2100 Copenhagen, Denmark
    Exp Cell Res 309:438-50. 2005
  6. pmc Phosphorylation and mRNA splicing of collapsin response mediator protein-2 determine inhibition of rho-associated protein kinase (ROCK) II function in carcinoma cell migration and invasion
    Marie Morgan-Fisher
    From the Department of Biomedical Sciences, Faculty of Health and Medical Sciences, and Biotech Research and Innovation Centre BRIC, University of Copenhagen, Ole Maaløes Vej 5, Copenhagen N, 2200, Denmark and
    J Biol Chem 288:31229-40. 2013
  7. ncbi request reprint Syndecans promote integrin-mediated adhesion of mesenchymal cells in two distinct pathways
    James R Whiteford
    National Heart and Lung Institute, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, Exhibition Road, London SW7 2AZ, UK
    Exp Cell Res 313:3902-13. 2007
  8. ncbi request reprint PKCbeta-dependent activation of RhoA by syndecan-4 during focal adhesion formation
    Athanassios Dovas
    Division of Biomedical Sciences, Imperial College London, London, SW7 2AZ, UK
    J Cell Sci 119:2837-46. 2006
  9. pmc Fibronectin matrix assembly requires distinct contributions from Rho kinases I and -II
    Atsuko Yoneda
    Division of Biomedical Sciences, Imperial College London, London SW7 2AZ, United Kingdom
    Mol Biol Cell 18:66-75. 2007
  10. doi request reprint Alpha9beta1 integrin in melanoma cells can signal different adhesion states for migration and anchorage
    Magnus C Lydolph
    Department of Biomedical Sciences, The Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark
    Exp Cell Res 315:3312-24. 2009

Collaborators

Detail Information

Publications35

  1. doi request reprint Transmembrane signaling proteoglycans
    John R Couchman
    Department of Biomedical Sciences, University of Copenhagen, Copenhagen, 2200 Denmark
    Annu Rev Cell Dev Biol 26:89-114. 2010
    ..Here some of the latest developments in the field are examined in hopes of stimulating further interest in this fascinating group of molecules...
  2. pmc Heparan sulfate chain valency controls syndecan-4 function in cell adhesion
    Sandeep Gopal
    Department of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark
    J Biol Chem 285:14247-58. 2010
    ....
  3. pmc Serine 34 phosphorylation of rho guanine dissociation inhibitor (RhoGDIalpha) links signaling from conventional protein kinase C to RhoGTPase in cell adhesion
    Athanassios Dovas
    Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, Exhibition Road, London SW7 2AZ, United Kingdom
    J Biol Chem 285:23296-308. 2010
    ..Together with fluorescence resonance energy transfer microscopy sensing GTP-RhoA levels, the data reveal a common pathway in cell adhesion linking two essential mediators, conventional PKC and RhoA...
  4. pmc An introduction to proteoglycans and their localization
    John R Couchman
    Department of Biomedical Sciences, University of Copenhagen, Copenhagen N, Denmark
    J Histochem Cytochem 60:885-97. 2012
    ..Included are some remarks on proteoglycan and glycosaminoglycan localization techniques, with reference to the special physicochemical properties of these complex molecules...
  5. ncbi request reprint Hierarchy of ADAM12 binding to integrins in tumor cells
    Charles Kumar Thodeti
    Institute of Molecular Pathology, University of Copenhagen, Frederik V s vej 11, DK 2100 Copenhagen, Denmark
    Exp Cell Res 309:438-50. 2005
    ..We demonstrated that phosphoinositide-3-kinase appears to be central in regulating alpha9beta1 integrin cell spreading activity in response to ADAM12...
  6. pmc Phosphorylation and mRNA splicing of collapsin response mediator protein-2 determine inhibition of rho-associated protein kinase (ROCK) II function in carcinoma cell migration and invasion
    Marie Morgan-Fisher
    From the Department of Biomedical Sciences, Faculty of Health and Medical Sciences, and Biotech Research and Innovation Centre BRIC, University of Copenhagen, Ole Maaløes Vej 5, Copenhagen N, 2200, Denmark and
    J Biol Chem 288:31229-40. 2013
    ....
  7. ncbi request reprint Syndecans promote integrin-mediated adhesion of mesenchymal cells in two distinct pathways
    James R Whiteford
    National Heart and Lung Institute, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, Exhibition Road, London SW7 2AZ, UK
    Exp Cell Res 313:3902-13. 2007
    ..Evidence for an indirect interaction of beta1 integrin with both syndecan ectodomains was obtained, all of which suggests a distinct mechanism of integrin-mediated adhesion...
  8. ncbi request reprint PKCbeta-dependent activation of RhoA by syndecan-4 during focal adhesion formation
    Athanassios Dovas
    Division of Biomedical Sciences, Imperial College London, London, SW7 2AZ, UK
    J Cell Sci 119:2837-46. 2006
    ..All these data point to a mechanism whereby syndecan-4 signals to RhoA in a PKCalpha-dependent manner and PKCalpha directly influences RhoA activity...
  9. pmc Fibronectin matrix assembly requires distinct contributions from Rho kinases I and -II
    Atsuko Yoneda
    Division of Biomedical Sciences, Imperial College London, London SW7 2AZ, United Kingdom
    Mol Biol Cell 18:66-75. 2007
    ..Together, two rho kinases contribute to fibronectin matrix assembly in a different manner and cortical myosin II-driven contractility, but not stress fibers, may be critical in this activity...
  10. doi request reprint Alpha9beta1 integrin in melanoma cells can signal different adhesion states for migration and anchorage
    Magnus C Lydolph
    Department of Biomedical Sciences, The Faculty of Health Sciences, University of Copenhagen, Copenhagen N, Denmark
    Exp Cell Res 315:3312-24. 2009
    ..Our results demonstrated that although alpha9beta1 integrin can induce and localise to focal adhesions in a high activation state, its intermediate activity state normally supports cell adhesion consistent with migration...
  11. ncbi request reprint A conserved NXIP motif is required for cell adhesion properties of the syndecan-4 ectodomain
    James R Whiteford
    Biomedical Sciences Division, Faculty of Natural Sciences, Imperial College London, Sir Alexander Fleming Building, Exhibition Road, London SW7 2AZ, United Kingdom
    J Biol Chem 281:32156-63. 2006
    ..We have shown that cell adhesion to the syndecan-4 ectodomain involves beta1 integrins in several cell types...
  12. pmc Structural and cell adhesion properties of zebrafish syndecan-4 are shared with higher vertebrates
    James R Whiteford
    Biomedicine Institute, University of Copenhagen, Biocenter, Ole Maaløes Vej 5, 2200 Copenhagen N, Denmark
    J Biol Chem 283:29322-30. 2008
    ..Therefore, the cell adhesion properties of syndecan-4 are consistent across the vertebrate spectrum and reflect an early acquisition of specialization after syndecan gene duplication events at the invertebrate/early chordate boundary...
  13. pmc The Rho kinases I and II regulate different aspects of myosin II activity
    Atsuko Yoneda
    Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, London SW7 2AZ, England, UK
    J Cell Biol 170:443-53. 2005
    ..ROCK II bound phosphatidylinositol 3,4,5P(3) and was sensitive to its levels, properties not shared by ROCK I. Therefore, endogenous ROCKs are distinctly regulated and in turn are involved with different myosin compartments...
  14. doi request reprint Mapping of matrix metalloproteinase cleavage sites on syndecan-1 and syndecan-4 ectodomains
    Tina Manon-Jensen
    Department of Biomedical Sciences, University of Copenhagen, Denmark
    FEBS J 280:2320-31. 2013
    ..The MT1-MMP cleavage sites in syndecan-1 and syndecan-4 were confirmed by site-directed mutagenesis. These findings provide insights into the characteristics of syndecan shedding...
  15. doi request reprint Heparan sulfate regulates ADAM12 through a molecular switch mechanism
    Hans Peter Sørensen
    Department of Biomedicine and Biotech Research and Innovation Centre, University of Copenhagen, Ole Maaløes Vej 5, DK 2200 Copenhagen N, Denmark
    J Biol Chem 283:31920-32. 2008
    ..These data present a novel concept that might allow targeting of ADAM12 and suggest that other ADAMs may have specific regulatory activity embedded in their prodomain and catalytic domain structures...
  16. doi request reprint The newcomer in the integrin family: integrin α9 in biology and cancer
    Anette M Høye
    Department of Biomedical Sciences, The Faculty of Health and Medical Sciences, and Biotech Research and Innovation Centre BRIC, University of Copenhagen, Copenhagen Biocenter, Ole Maaløes Vej 5, Copenhagen N 2200, Denmark
    Adv Biol Regul 52:326-39. 2012
    ..This review gives an overview of the recent progress in α9β1-mediated biological and pathological processes, and discusses its potential as a target for cancer diagnosis and therapy...
  17. ncbi request reprint Cellular adhesion responses to the heparin-binding (HepII) domain of fibronectin require heparan sulfate with specific properties
    Yashithra Mahalingam
    Division of Biomedical Sciences, Imperial College London, London SW7 2AZ, United Kingdom
    J Biol Chem 282:3221-30. 2007
    ..Several separate, specific properties of cell surface HS are therefore required in cell adhesion responses to the fibronectin HepII domain...
  18. pmc A collapsin response mediator protein 2 isoform controls myosin II-mediated cell migration and matrix assembly by trapping ROCK II
    Atsuko Yoneda
    Department of Biomedical Sciences, Faculty of Health Sciences, and Biotech Research and Innovation Centre, University of Copenhagen, Copenhagen, Denmark
    Mol Cell Biol 32:1788-804. 2012
    ..This study provides a new insight into CRMP-2 as a controller of myosin II-mediated cellular functions through the inhibition of ROCK II in nonneuronal cells...
  19. ncbi request reprint Cellular roles of ADAM12 in health and disease
    Marie Kveiborg
    Department of Biomedical Sciences and Biotech Research and Innovation Centre, The Faculty of Health Sciences, Copenhagen University, Copenhagen Biocenter, Ole Maaløesvej 5, 2200 Copenhagen N, Denmark
    Int J Biochem Cell Biol 40:1685-702. 2008
    ....
  20. doi request reprint Proteoglycans in health and disease: the multiple roles of syndecan shedding
    Tina Manon-Jensen
    Deparment of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark
    FEBS J 277:3876-89. 2010
    ..Here, recent research into the shedding of syndecans and its physiological relevance are assessed...
  21. pmc Protein kinase C, focal adhesions and the regulation of cell migration
    Betina S Fogh
    Department of Biomedical Sciences, University of Copenhagen, Denmark
    J Histochem Cytochem 62:172-84. 2014
    ..Here the status of protein kinase C in focal adhesions and cell migration is reviewed, together with discussion of its roles and potential substrates. ..
  22. pmc RhoGDI: multiple functions in the regulation of Rho family GTPase activities
    Athanassios Dovas
    Division of Biomedical Sciences, Imperial College London, Exhibition Road, London SW7 2AZ, UK
    Biochem J 390:1-9. 2005
    ..All these results point to a wider role for RhoGDI than initially perceived, making it a binding partner that can tightly control Rho GTPases, but which also allows them to reach their full spectrum of activities...
  23. ncbi request reprint Syndecans-2 and -4; close cousins, but not identical twins
    Eok Soo Oh
    Division of Biomedical Sciences, Imperial College London, Exhibition Road, London SW7 2AZ, United Kingdom
    Mol Cells 17:181-7. 2004
    ....
  24. ncbi request reprint Regulation of cytoskeletal organization by syndecan transmembrane proteoglycans
    Atsuko Yoneda
    Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, Exhibition Road, UK
    Matrix Biol 22:25-33. 2003
    ..Here we will review current understanding of signaling through syndecans in cytoskeletal organization...
  25. pmc Syndecan-1 and syndecan-4 are independent indicators in breast carcinoma
    Maria E Lendorf
    Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark
    J Histochem Cytochem 59:615-29. 2011
    ..These syndecans may, therefore, have distinct roles in regulating breast carcinoma cell behavior...
  26. ncbi request reprint Syndecans: proteoglycan regulators of cell-surface microdomains?
    John R Couchman
    Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, Exhibition Road, London SW7 2AZ, UK
    Nat Rev Mol Cell Biol 4:926-37. 2003
  27. doi request reprint Syndecans as receptors and organizers of the extracellular matrix
    Xiaojie Xian
    Department of Biomedical Sciences, University of Copenhagen, Biocenter, Denmark
    Cell Tissue Res 339:31-46. 2010
    ..Developmental deficits in lower vertebrates in which syndecans are eliminated are also informative and suggest that, in mammals, redundancy is a key issue...
  28. pmc Breast and ovarian cancers: a survey and possible roles for the cell surface heparan sulfate proteoglycans
    Atsuko Yoneda
    Department of Biomedical Sciences, University of Copenhagen, Denmark
    J Histochem Cytochem 60:9-21. 2012
    ..Both families of proteoglycans have been implicated in cellular responses to growth factors and morphogens, including many now associated with tumor progression...
  29. ncbi request reprint ADAM12/syndecan-4 signaling promotes beta 1 integrin-dependent cell spreading through protein kinase Calpha and RhoA
    Charles Kumar Thodeti
    Institute of Molecular Pathology, University of Copenhagen, Frederik V s vej 11, DK 2100, Copenhagen, Denmark
    J Biol Chem 278:9576-84. 2003
    ..In summary, these data provide novel insights into syndecan-4 signaling. Syndecan-4 can promote cell spreading in a beta(1) integrin-dependent fashion through PKCalpha and RhoA, and PKCalpha and RhoA likely function in separate pathways...
  30. ncbi request reprint Syndecans in wound healing, inflammation and vascular biology
    Annika N Alexopoulou
    Division of Biomedical Sciences, Imperial College London, Sir Alexander Fleming Building, Exhibition Road, London SW7 2AZ, UK
    Int J Biochem Cell Biol 39:505-28. 2007
    ....
  31. doi request reprint An epidermal microRNA regulates neuronal migration through control of the cellular glycosylation state
    Mikael Egebjerg Pedersen
    Biotech Research and Innovation Centre, University of Copenhagen, Ole Maaløes Vej 5, Copenhagen, Denmark
    Science 341:1404-8. 2013
    ..Our results identify a regulatory axis controlled by a conserved microRNA that maintains proteoglycan homeostasis in cells. ..
  32. pmc Two distinct sites in sonic Hedgehog combine for heparan sulfate interactions and cell signaling functions
    Shu Chun Chang
    Molecular Medicine Section, National Heart and Lung Institute, Imperial College London, South Kensington, London SW7 2AZ, United Kingdom
    J Biol Chem 286:44391-402. 2011
    ..These studies provide a new insight into the functional roles of hShh interactions with HSPGs, which may allow targeting this aspect of hShh biology in, for example, pancreatic ductal adenocarcinoma...
  33. pmc Attenuation of hedgehog acyltransferase-catalyzed sonic Hedgehog palmitoylation causes reduced signaling, proliferation and invasiveness of human carcinoma cells
    Antonios D Konitsiotis
    Molecular Medicine Section, National Heart and Lung Institute, Imperial College London, London, United Kingdom
    PLoS ONE 9:e89899. 2014
    ..Our data identify Hhat as a key player in Hh-dependent signaling and tumour cell transformed behaviour. ..
  34. pmc Heparan sulfate biosynthesis: methods for investigation of the heparanosome
    Hinke A B Multhaupt
    Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark
    J Histochem Cytochem 60:908-15. 2012
    ....
  35. pmc Commercial antibodies: the good, bad, and really ugly
    John R Couchman
    Biomedicine Institute, University of Copenhagen, Biocenter, Ole Maaloes Vej 5, 2200 Copenhagen N, Denmark
    J Histochem Cytochem 57:7-8. 2009
    ..Additionally, companies should provide the necessary information about the antigen and antibody to investigators, including references, so that the appropriate controls can be included...