Alessandro Costa



  1. Goswami P, Abid Ali F, Douglas M, Locke J, Purkiss A, Janska A, et al. Structure of DNA-CMG-Pol epsilon elucidates the roles of the non-catalytic polymerase modules in the eukaryotic replisome. Nat Commun. 2018;9:5061 pubmed publisher
    ..At least some of these contacts are preserved during Pol epsilon-dependent CMG assembly on path to origin firing, as observed with DNA replication reconstituted in vitro. ..
  2. Goswami P, Locke J, Costa A. Preparing Frozen-Hydrated Protein-Nucleic Acid Assemblies for High-Resolution Cryo-EM Imaging. Methods Mol Biol. 2018;1814:287-296 pubmed publisher
    ..In this book chapter we describe our protocols for negative-stain grid and cryo-grid preparation, which we apply to studying protein-nucleic acid complexes. ..
  3. Pellegrini L, Costa A. New Insights into the Mechanism of DNA Duplication by the Eukaryotic Replisome. Trends Biochem Sci. 2016;41:859-871 pubmed publisher
  4. Abid Ali F, Costa A. The MCM Helicase Motor of the Eukaryotic Replisome. J Mol Biol. 2016;428:1822-32 pubmed publisher
    ..Despite the exciting progress, how the MCM translocates on DNA remains a matter of debate. ..
  5. Swuec P, Costa A. DNA replication and inter-strand crosslink repair: Symmetric activation of dimeric nanomachines?. Biophys Chem. 2017;225:15-19 pubmed publisher
  6. Eickhoff P, Costa A. Escorting Client Proteins to the Hsp90 Molecular Chaperone. Structure. 2017;25:964-965 pubmed publisher
    ..In this issue of Structure, Rivera-Calzada, Pal et al. (2017) describe the architecture and catalytic properties of R2TP, providing new insights into the interplay between Hsp90 and its co-chaperones. ..
  7. Abid Ali F, Douglas M, Locke J, Pye V, NANS A, Diffley J, et al. Cryo-EM structure of a licensed DNA replication origin. Nat Commun. 2017;8:2241 pubmed publisher
    ..By comparing our new MCM-DNA structure with the structure of CMG-DNA, we suggest how the conformational transition from the loaded, post-catalytic MCM to CMG might promote DNA untwisting and melting at the onset of replication. ..
  8. Swuec P, Renault L, Borg A, Shah F, Murphy V, van Twest S, et al. The FA Core Complex Contains a Homo-dimeric Catalytic Module for the Symmetric Mono-ubiquitination of FANCI-FANCD2. Cell Rep. 2017;18:611-623 pubmed publisher
    ..A transient interaction with FANCC-FANCE-FANCF alters the FANCI-FANCD2 configuration, stabilizing the dimerization interface. Our data provide a model to explain how equivalent mono-ubiquitination of FANCI and FANCD2 occurs. ..
  9. Zhou J, Janska A, Goswami P, Renault L, Abid Ali F, Kotecha A, et al. CMG-Pol epsilon dynamics suggests a mechanism for the establishment of leading-strand synthesis in the eukaryotic replisome. Proc Natl Acad Sci U S A. 2017;114:4141-4146 pubmed publisher
    ..We observe two alternate configurations of the DNA synthesis domain in the CMG-bound Pol epsilon. We propose that this conformational switch might control DNA template engagement and release, modulating replisome progression. ..


More Information


  1. Miller T, Costa A. The architecture and function of the chromatin replication machinery. Curr Opin Struct Biol. 2017;47:9-16 pubmed publisher
    ..The emerging picture indicates that the core eukaryotic DNA replication machinery has evolved elements that handle nucleosomes to facilitate chromatin duplication. ..