Ronald C Conaway

Summary

Publications

  1. pmc The Mediator complex and transcription elongation
    Ronald C Conaway
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Biochim Biophys Acta 1829:69-75. 2013
  2. pmc Origins and activity of the Mediator complex
    Ronald C Conaway
    Stowers Institute for Medical Research, 1000 E 50th Street, Kansas City, MO 64110, USA
    Semin Cell Dev Biol 22:729-34. 2011
  3. pmc Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling
    Lu Chen
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 286:11283-9. 2011
  4. ncbi request reprint The mammalian YL1 protein is a shared subunit of the TRRAP/TIP60 histone acetyltransferase and SRCAP complexes
    Yong Cai
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 280:13665-70. 2005
  5. pmc Distinct modes of regulation of the Uch37 deubiquitinating enzyme in the proteasome and in the Ino80 chromatin-remodeling complex
    Tingting Yao
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Mol Cell 31:909-17. 2008
  6. ncbi request reprint A mammalian chromatin remodeling complex with similarities to the yeast INO80 complex
    Jingji Jin
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 280:41207-12. 2005
  7. ncbi request reprint A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2
    Chieri Tomomori-Sato
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 279:5846-51. 2004
  8. ncbi request reprint YY1 functions with INO80 to activate transcription
    Yong Cai
    Stowers Institute for Medical Research, 1000 E 50th Street, Kansas City, Missouri 64110, USA
    Nat Struct Mol Biol 14:872-4. 2007
  9. ncbi request reprint A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology
    Shigeo Sato
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Mol Cell 14:685-91. 2004
  10. ncbi request reprint A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex
    Shigeo Sato
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 278:49671-4. 2003

Collaborators

Detail Information

Publications42

  1. pmc The Mediator complex and transcription elongation
    Ronald C Conaway
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Biochim Biophys Acta 1829:69-75. 2013
    ..Although Mediator was initially found to play a critical role in the regulation of the initiation of Pol II transcription, recent studies have brought to light an expanded role for Mediator at post-initiation stages of transcription...
  2. pmc Origins and activity of the Mediator complex
    Ronald C Conaway
    Stowers Institute for Medical Research, 1000 E 50th Street, Kansas City, MO 64110, USA
    Semin Cell Dev Biol 22:729-34. 2011
    ..Here we provide a brief overview of the evolutionary origins of Mediator, its subunit composition, and its remarkably diverse collection of activities in Pol II transcription...
  3. pmc Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling
    Lu Chen
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 286:11283-9. 2011
    ..Taken together, our findings shed new light on the structure and function of the INO80 chromatin-remodeling complex...
  4. ncbi request reprint The mammalian YL1 protein is a shared subunit of the TRRAP/TIP60 histone acetyltransferase and SRCAP complexes
    Yong Cai
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 280:13665-70. 2005
    ..Taken together, our findings identify the YL1 protein as a new subunit of the TRRAP/TIP60 HAT complex, and they suggest that YL1 plays multiple roles in chromatin modification and remodeling in cells...
  5. pmc Distinct modes of regulation of the Uch37 deubiquitinating enzyme in the proteasome and in the Ino80 chromatin-remodeling complex
    Tingting Yao
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Mol Cell 31:909-17. 2008
    ..In addition, our findings suggest that the proteasome and the hINO80 chromatin-remodeling complex may cooperate to regulate transcription or DNA repair, processes in which both complexes have been implicated...
  6. ncbi request reprint A mammalian chromatin remodeling complex with similarities to the yeast INO80 complex
    Jingji Jin
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 280:41207-12. 2005
    ..Finally, we demonstrate that, similar to the yeast INO80 complex, the hINO80 complex exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding...
  7. ncbi request reprint A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2
    Chieri Tomomori-Sato
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 279:5846-51. 2004
    ..Taken together, our findings identify a novel mammalian Mediator subunit and shed new light on the architecture of the mammalian Mediator complex...
  8. ncbi request reprint YY1 functions with INO80 to activate transcription
    Yong Cai
    Stowers Institute for Medical Research, 1000 E 50th Street, Kansas City, Missouri 64110, USA
    Nat Struct Mol Biol 14:872-4. 2007
    ..Binding of YY1 to its DNA sites in target genes requires INO80, suggesting that YY1 uses the INO80 complex not only to activate transcription but also to gain access to target promoters...
  9. ncbi request reprint A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology
    Shigeo Sato
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Mol Cell 14:685-91. 2004
    ....
  10. ncbi request reprint A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex
    Shigeo Sato
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 278:49671-4. 2003
    ..Taken together, our findings identify a new subunit of the mammalian Mediator and shed new light on the architecture of the mammalian Mediator complex...
  11. pmc Poly(ADP-ribosyl)ation directs recruitment and activation of an ATP-dependent chromatin remodeler
    Aaron J Gottschalk
    The Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Proc Natl Acad Sci U S A 106:13770-4. 2009
    ..We propose that poly(ADP-ribosyl)ation of chromatin-associated Parp1 serves as a mechanism for targeting a SNF2 family remodeler to chromatin...
  12. ncbi request reprint Identification of Elongin C and Skp1 sequences that determine Cullin selection
    Qin Yan
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 279:43019-26. 2004
    ....
  13. doi request reprint Characterization of Cullin-box sequences that direct recruitment of Cul2-Rbx1 and Cul5-Rbx2 modules to Elongin BC-based ubiquitin ligases
    Nawel Mahrour
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 283:8005-13. 2008
    ..Taken together, our findings shed new light on the mechanisms by which BC-box proteins direct recruitment of Cullin-Rbx modules during reconstitution of ubiquitin ligases...
  14. pmc Probabilistic assembly of human protein interaction networks from label-free quantitative proteomics
    Mihaela E Sardiu
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Proc Natl Acad Sci U S A 105:1454-9. 2008
    ..Finally, we constructed a probabilistic human interaction network composed of 557 protein pairs...
  15. pmc ELL-associated factors 1 and 2 are positive regulators of RNA polymerase II elongation factor ELL
    Stephanie E Kong
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Proc Natl Acad Sci U S A 102:10094-8. 2005
    ..Our findings provide insights into the structure and function of ELL family transcription factors, and they bring to light direct roles for the EAF proteins in regulation of RNA polymerase II transcription...
  16. ncbi request reprint The mammalian Mediator complex
    Joan Weliky Conaway
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    FEBS Lett 579:904-8. 2005
    ..Here we discuss efforts to characterize the subunit composition and associated activities of the mammalian Med complex...
  17. ncbi request reprint Proteasome recruitment and activation of the Uch37 deubiquitinating enzyme by Adrm1
    Tingting Yao
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Nat Cell Biol 8:994-1002. 2006
    ..However, neither Uch37 alone nor the Uch37-Adrm1 or Uch37-Adrm1-S1 complexes can hydrolyse di-ubiquitin efficiently; rather, incorporation into the 19S complex is required to enable processing of polyubiquitin chains...
  18. pmc Human mediator subunit MED26 functions as a docking site for transcription elongation factors
    Hidehisa Takahashi
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Cell 146:92-104. 2011
    ....
  19. ncbi request reprint Identification and Characterization of a Schizosaccharomyces pombe RNA Polymerase II Elongation Factor with Similarity to the Metazoan Transcription Factor ELL
    Charles A S Banks
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 282:5761-9. 2007
    ..pombe sensitive to the drug 6-azauracil. Finally, phylogenetic analyses suggest that the SpELL and SpEAF proteins are evolutionarily conserved in many fungi but not in Saccharomyces cerevisiae...
  20. pmc AFF4, a component of the ELL/P-TEFb elongation complex and a shared subunit of MLL chimeras, can link transcription elongation to leukemia
    Chengqi Lin
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Mol Cell 37:429-37. 2010
    ..Knockdown of AFF4 in leukemic cells shows reduction in MLL chimera target gene expression, suggesting that AFF4/SEC could be a key regulator in the pathogenesis of leukemia through many of the MLL partners...
  21. ncbi request reprint Identification of new subunits of the multiprotein mammalian TRRAP/TIP60-containing histone acetyltransferase complex
    Yong Cai
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 278:42733-6. 2003
    ..Taken together, our findings shed new light on the structure and function of the mammalian TRRAP/TIP60 histone acetyltransferase complex...
  22. pmc Purification and assay of the human INO80 and SRCAP chromatin remodeling complexes
    Yong Cai
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Methods 40:312-7. 2006
    ..Z into nucleosomes. Here we describe methods for purification and assay of the INO80 and SRCAP chromatin remodeling complexes...
  23. ncbi request reprint Preparation and assay of RNA polymerase II elongation factors elongin and ELL
    Stephanie E Kong
    Stowers Institute Medical Research, 1000 E 50th Street, Kansas City, Missouri 64110, USA
    Methods Enzymol 371:276-83. 2003
  24. ncbi request reprint The mammalian Mediator complex and its role in transcriptional regulation
    Ronald C Conaway
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Trends Biochem Sci 30:250-5. 2005
    ..Recent studies have defined the subunit composition and associated activities of mammalian Mediator, and revealed a striking evolutionary conservation of Mediator structure and function from yeast to man...
  25. ncbi request reprint Neuralized-like 1 (Neurl1) targeted to the plasma membrane by N-myristoylation regulates the Notch ligand Jagged1
    Evangelia Koutelou
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 283:3846-53. 2008
    ..Taken together, our results argue that Neurl1 at the plasma membrane can affect the signaling activity of Jagged1 by directly enhancing its ubiquitination and subsequent turnover...
  26. ncbi request reprint Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3
    Shigeo Sato
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 278:15123-7. 2003
    ..Taken together, our findings identify a collection of novel mammalian Mediator subunits and shed new light on the underlying architecture of the mammalian Mediator complex...
  27. pmc Subunit composition and substrate specificity of a MOF-containing histone acetyltransferase distinct from the male-specific lethal (MSL) complex
    Yong Cai
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 285:4268-72. 2010
    ..Although MSL-associated MOF acetylates nucleosomal histone H4 almost exclusively on lysine 16, NSL-associated MOF exhibits a relaxed specificity and also acetylates nucleosomal histone H4 on lysines 5 and 8...
  28. pmc Proteomics reveals a physical and functional link between hepatocyte nuclear factor 4alpha and transcription factor IID
    Hidehisa Takahashi
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 284:32405-12. 2009
    ..These findings identify an unexpected role for the HNF4alpha DNA-binding domain in mediating key regulatory interactions and provide new insights into the roles of HNF4alpha and TFIID in RNA polymerase II transcription...
  29. ncbi request reprint In and out: histone variant exchange in chromatin
    Jingji Jin
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Trends Biochem Sci 30:680-7. 2005
    ..The incorporation of histone variants most probably has epigenetic consequences and contributes to architectural changes in chromosomes...
  30. pmc Quantitative proteomic analysis of distinct mammalian Mediator complexes using normalized spectral abundance factors
    Andrew C Paoletti
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Proc Natl Acad Sci U S A 103:18928-33. 2006
    ..This method of protein complex visualization has important implications for the analysis of multiprotein complexes and assembly of protein interaction networks...
  31. pmc Multiple modes of regulation of the human Ino80 SNF2 ATPase by subunits of the INO80 chromatin-remodeling complex
    Lu Chen
    Stowers Institute for Medical Research, Kansas City, MO 64110
    Proc Natl Acad Sci U S A 110:20497-502. 2013
    ..These findings support the idea that both substrate recognition and the intrinsic catalytic activities of SNF2 ATPases have evolved as important sites for their regulation. ..
  32. pmc Activation of the SNF2 family ATPase ALC1 by poly(ADP-ribose) in a stable ALC1·PARP1·nucleosome intermediate
    Aaron J Gottschalk
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 287:43527-32. 2012
    ..Taken together, our findings are consistent with the model that PAR present on PARylated PARP1 acts as an allosteric effector of ALC1 nucleosome remodeling activity...
  33. ncbi request reprint The von Hippel-Lindau tumor suppressor complex and regulation of hypoxia-inducible transcription
    Ronald C Conaway
    The Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Adv Cancer Res 85:1-12. 2002
  34. pmc Role for human mediator subunit MED25 in recruitment of mediator to promoters by endoplasmic reticulum stress-responsive transcription factor ATF6α
    Dotan Sela
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 288:26179-87. 2013
    ..We present findings arguing that Mediator subunit MED25 plays a critical role in this process and identify a MED25 domain that serves as a docking site on Mediator for the ATF6α transcription activation domain. ..
  35. pmc Endoplasmic reticulum stress-responsive transcription factor ATF6α directs recruitment of the Mediator of RNA polymerase II transcription and multiple histone acetyltransferase complexes
    Dotan Sela
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 287:23035-45. 2012
    ....
  36. doi request reprint The INO80 chromatin remodeling complex in transcription, replication and repair
    Ronald C Conaway
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA Department of Biochemistry and Molecular Biology, Kansas University Medical Center, Kansas City, KS 66160, USA
    Trends Biochem Sci 34:71-7. 2009
    ..Recent studies have shown that the INO80 complex contributes to a wide variety of chromatin-dependent nuclear transactions, including transcription, DNA repair and DNA replication...
  37. pmc Immunoaffinity purification of protein complexes from Mammalian cells
    Chieri Tomomori-Sato
    Stowers Institute for Medical Research, Kansas City, MO, USA
    Methods Mol Biol 977:273-87. 2013
    ..As an example we describe isolation of the mammalian Mediator complex from HeLa S3 cells...
  38. ncbi request reprint TFIIS and GreB: two like-minded transcription elongation factors with sticky fingers
    Ronald C Conaway
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Cell 114:272-4. 2003
  39. pmc Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase
    Christopher S Brower
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Proc Natl Acad Sci U S A 99:10353-8. 2002
    ..Taken together, our findings are consistent with the model that MED8 could function to recruit ubiquitin ligase activity directly to the RNA polymerase II transcriptional machinery...
  40. pmc Function and regulation of the Mediator complex
    Ronald C Conaway
    Stowers Institute for Medical Research, 1000 E 50th Street, Kansas City, MO 64110, USA
    Curr Opin Genet Dev 21:225-30. 2011
    ....
  41. ncbi request reprint Emerging roles of ubiquitin in transcription regulation
    Ronald C Conaway
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Science 296:1254-8. 2002
    ....
  42. ncbi request reprint Mediator comes out from the shadows
    Ronald C Conaway
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA Department of Biochemistry and Molecular Biology, Kansas University Medical Center, Kansas City, KS 66160, USA
    Structure 17:485-6. 2009
    ..The improved Mediator structural model highlights the remarkable conformational flexibility of Mediator and reveals previously unrecognized features of Mediator modules...